1. Highly Attenuated Infection With a Vpr-Deleted Molecular Clone of Human Immunodeficiency Virus-1
- Author
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Ali, Ayub, Ng, Hwee L, Blankson, Joel N, Burton, Dennis R, Buckheit, Robert W, Moldt, Brian, Fulcher, Jennifer A, Ibarrondo, F Javier, Anton, Peter A, and Yang, Otto O
- Subjects
Medical Microbiology ,Biomedical and Clinical Sciences ,Immunology ,Biotechnology ,Vaccine Related ,HIV/AIDS ,Prevention ,Sexually Transmitted Infections ,Vaccine Related (AIDS) ,Infectious Diseases ,Immunization ,2.1 Biological and endogenous factors ,Infection ,Good Health and Well Being ,AIDS Vaccines ,B-Lymphocytes ,CD8-Positive T-Lymphocytes ,Cloning ,Molecular ,Female ,Gene Products ,vpr ,HIV Infections ,HIV-1 ,Humans ,Middle Aged ,Vaccination ,Vaccines ,Attenuated ,elite control ,vpr ,Biological Sciences ,Medical and Health Sciences ,Microbiology ,Biological sciences ,Biomedical and clinical sciences ,Health sciences - Abstract
A 48-year-old woman was infected with a vpr-defective human immunodeficiency virus (HIV)-1 molecular clone. Seroconversion was markedly delayed, and without treatment she had durably suppressed viremia and normal T-cell levels. Neutralizing antibody and CD8+ T-cell immune responses against HIV-1 were unremarkable. Viral sequences confirmed the source but evolved defective nef, suggesting an unknown mechanistic link to vpr. There were subtle qualitative defects in T and B cells. To our knowledge, this is the only case of human infection with a characterized defective HIV-1 molecular clone, which furthermore recapitulated live-attenuated vaccination in macaque models of HIV-1 vaccine research.
- Published
- 2018