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Your search keyword '"Bu, Yingcui"' showing total 34 results
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34 results on '"Bu, Yingcui"'

8. Simultaneous two-color visualization of lipid droplets and lysosomes for cell homeostasis monitoring using a single fluorescent probe.

Author

Liu, Mengxiao, Bu, Yingcui, Wang, Dongxiao, Tang, Lihua, Hu, Didi, Li, Longchun, and Gan, Xiaoping

Subjects
*INTRAMOLECULAR charge transfer, *FLUORESCENT probes, *STOKES shift, *LYSOSOMES, *ORGANELLES
Abstract

Lipid droplets (LDs) and lysosomes are vital organelles that play crucial roles in various physiological and pathological processes. However, simultaneous two-color visualization of these two organelles using a single probe for cell homeostasis monitoring remains a challenge due to the lack of rational design strategies. To address this issue, we have developed an aggregation-induced emission (AIE) fluorescent probe named TPE-NDI-Mor with an electron donor (D)–acceptor (A) structure, which can stain both LDs and lysosomes with high selectivity through green and red fluorescence imaging, respectively. A detailed mechanism study revealed that TPE-NDI-Mor, with a twisted intramolecular charge transfer (TICT) effect, shows a high affinity for a polar microenvironment. Additionally, the probe also demonstrates good stability, high anti-interference performance and a large Stokes shift, making it suitable for visualizing cell homeostasis and further disease diagnosis by tracking the dynamic changes of LDs and lysosomes. [ABSTRACT FROM AUTHOR]

Published
2024
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29. Photo-Activated Ratiometric Fluorescent Indicator for Real-Time and Visual Detection of Plasma Membrane Homeostasis

Author

Bu, Yingcui, Wang, Haoran, Deng, Yu, Zhong, Feng, Yu, Zhi-peng, Zhu, Xiaojiao, and Zhou, Hongping

Abstract

Development of an activated ratiometric indicator that is specific to plasma membrane (PM) viscosity exhibits great application prospects in disease diagnosis and treatment but remains a great challenge. Herein, a photo-activated fluorescent probe (CQ-IC) was designed and prepared tactfully, which could analyze and real-time monitor the microenvironmental homeostasis of the PM based on a two-channel ratiometric imaging model. Interestingly, upon light irradiation, CQ-ICgenerates reactive oxygen species and thus increases the cellular viscosity, which increases two emission peaks at 480 and 610 nm. This work would propose a new strategy to sensor PM homeostasis and effectively guide the treatment of viscosity-related diseases among various physiological and pathological processes.

Published
2024
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31. Bifunctional Single-Molecular Fluorescent Probe: Visual Detection of Mitochondrial SO2and Membrane Potential

Author

Ai, Wenming, Bu, Yingcui, Huang, Houshi, Wang, Junjun, Ren, Mengjuan, Deng, Yu, Zhu, Yicai, Wang, Sen, Yu, Zhi-Peng, and Zhou, Hongping

Abstract

Mitochondrial membrane potential (MMP) and sulfur dioxide (SO2) significantly affect the mitochondrial state. In this work, TC-2and TC-8were constructed through side-chain engineering, in which TC-2bearing the poorer hydrophobicity could localize on mitochondria better. Interestingly, short-wave emission was captured due to the sensitive response of TC-2to SO2(LOD = 13.8 nM). Meanwhile, the probe could bind with DNA, presenting enhanced long-wave emission. Encouragingly, TC-2could migrate from mitochondria to the nucleus when MMP was decreased, accompanied by the increase of fluorescence lifetime (9-fold). Hence, TC-2could be used for dual-channel monitoring of mitochondrial SO2and MMP, which showed a completely different pathway from the commercial MMP detectors JC-1/JC-10. The cellular experiments showed that MMP was gradually decreased due to reactive oxygen species-triggered oxidative stress, and the SO2level was up-regulated simultaneously. Overall, this work proposed a new method to investigate and diagnose the mitochondrial-related diseases.

Published
2023
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32. Solvatochromic Two-Photon Fluorescent Probe Enables In SituLipid Droplet Multidynamics Tracking for Nonalcoholic Fatty Liver and Inflammation Diagnoses

Author

Huang, Houshi, Bu, Yingcui, Yu, Zhi-Peng, Rong, Mengtao, Li, Rui, Wang, Zhijie, Zhang, Jie, Zhu, Xiaojiao, Wang, Lianke, and Zhou, Hongping

Abstract

Intracellular lipid storage and regulation occur in lipid droplets, which are of great significance to the physiological activities of cells. Herein, a lipid droplet-specific fluorescence probe (lip-YB) with a high quantum yield (QYlip-YB= 73.28%), excellent photostability, and quickly polarity sensitivity was constructed successfully. Interestingly, lip-YBexhibited remarkable two-photon (TP) characteristics, which first realized real-time monitoring of the lipid droplet multidynamics process, diagnosing nonalcoholic fatty liver disease (NAFLD) and inflammation in living mice via TP fluorescence imaging. It is found that the as-prepared lip-YBprovides a new avenue to design lipid droplet-specific imaging probes, clarifies its roles and mechanisms in cell metabolism, and can timely intervene in lipid droplet-related diseases during various physiological and pathological processes.

Published
2022
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33. Enhanced pH-Responsive Chemo/Chemodynamic Synergistic Cancer Therapy Based on In Situ Cu 2+ Di-Chelation.

Author

Zhou M, Tian B, Bu Y, Wu Z, Yu J, Wang S, Sun X, Zhu X, and Zhou H

Subjects
Animals, Mice, Mitoxantrone, Tumor Microenvironment, Hydrogen-Ion Concentration, Copper, Neoplasms drug therapy
Abstract

Considering the chemodynamic therapy and chemotherapy independent of external stimulus witnessing great advantage in the clinical translation, developing a smart nanoplatform that can realize enhanced chemo/chemodynamic synergistic therapy in the tumor microenvironment (TME) is of great significance. Herein, we highlight the enhanced pH-responsive chemo/chemodynamic synergistic cancer therapy based on in situ Cu 2+ di-chelation. The alcohol-withdrawal drug disulfiram (DSF) and chemotherapeutic drug mitoxantrone (MTO) were embedded into PEGylated mesoporous CuO (denoted as PEG-CuO@DSF@MTO NPs). The acidic TME triggered the collapse of CuO and the concurrent release of Cu 2+ , DSF, and MTO. Then, the in situ complexation between Cu 2+ and DSF, as well as the coordination between Cu 2+ and MTO not only prominently enhanced the chemotherapeutic performance but also triggered the chemodynamic therapy. In vivo mouse model experiments demonstrated that the synergistic therapy can remarkably eliminate tumors. This study provides an interesting strategy to design intelligent nanosystems, which could proceed to clinical translations.

Published
2023
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34. Bifunctional Single-Molecular Fluorescent Probe: Visual Detection of Mitochondrial SO 2 and Membrane Potential.

Author

Ai W, Bu Y, Huang H, Wang J, Ren M, Deng Y, Zhu Y, Wang S, Yu ZP, and Zhou H

Subjects
Humans, Membrane Potentials, Sulfur Dioxide metabolism, HeLa Cells, Membrane Potential, Mitochondrial, Fluorescent Dyes metabolism, Mitochondria metabolism
Abstract

Mitochondrial membrane potential (MMP) and sulfur dioxide (SO 2 ) significantly affect the mitochondrial state. In this work, TC-2 and TC-8 were constructed through side-chain engineering, in which TC-2 bearing the poorer hydrophobicity could localize on mitochondria better. Interestingly, short-wave emission was captured due to the sensitive response of TC-2 to SO 2 (LOD = 13.8 nM). Meanwhile, the probe could bind with DNA, presenting enhanced long-wave emission. Encouragingly, TC-2 could migrate from mitochondria to the nucleus when MMP was decreased, accompanied by the increase of fluorescence lifetime (9-fold). Hence, TC-2 could be used for dual-channel monitoring of mitochondrial SO 2 and MMP, which showed a completely different pathway from the commercial MMP detectors JC-1/JC-10. The cellular experiments showed that MMP was gradually decreased due to reactive oxygen species-triggered oxidative stress, and the SO 2 level was up-regulated simultaneously. Overall, this work proposed a new method to investigate and diagnose the mitochondrial-related diseases.

Published
2023
Full Text
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