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The bioinsecticidal effect of mutant Bacillus thuringiensis H-14 var israelensis (Bti) on Aedes aegypti III larvae was evaluated by exposing them to three concentrations of the mutant Bti (16 560, 33 120 and 66 240 esp/mL), each treatment had 25 larvae in stage III of A. aegypti, which were exposed to different concentrations of mutant Bti and a control group. Bioassays between A. aegypti and mutant Bti were performed under laboratory conditions, evaluated at 24, 48, and 72 hours after exposure. The results showed that the higher the concentration of mutant Bti, the greater the larval mortality that occurred, with the concentration of 66 240 esp/mL being the most effective at 72 hours. It is concluded that mutant Bacillus thuringiensis H-14 var. israelensis has a high larvicidal activity against Aedes aegypti under laboratory conditions. [ABSTRACT FROM AUTHOR]