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130 results on '"Brzozka, Z."'

18. A549 and MRC-5 cell aggregation in a microfluidic Lab-on-a-chip system.

Author

Zuchowska, A., Jastrzebska, E., Zukowski, K., Chudy, M., Dybko, A., and Brzozka, Z.

Subjects
CELL aggregation, MICROFLUIDICS, CELL-mediated cytotoxicity, CELL culture, LABS on a chip, DRUG toxicity
Abstract

In this paper, we present a culture of A549 and MRC-5 spheroids in a microfluidic system. The aim of our work was to develop a good lung cancer model for the evaluation of drug cytotoxicity. Our research was focused on determining the progress of cell aggregation depending on such factors as the depth of culture microwells in the microdevices, a different flow rate of the introduced cell suspensions, and the addition of collagen to cell suspensions. We showed that these factors had a significant influence on spheroid formation. It was found that both MRC-5 and A549 cells exhibited higher aggregation in 500 µm microwells. We also noticed that collagen needs to be added to A549 cells to form the spheroids. Optimizing the mentioned parameters allowed us to form 3D lung tissue models in the microfluidic system during the 10-day culture. This study indicates how important an appropriate selection of the specified parameters is (e.g., geometry of the microwells in the microsystem) to obtain the spheroids characterized by high viability in the microfluidic system. [ABSTRACT FROM AUTHOR]

Published
2017
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25. CHEMICALLY MODIFIED FIELD-EFFECT TRANSISTORS - POTENTIOMETRIC AG+ SELECTIVITY OF PVC MEMBRANES BASED ON MACROCYCLIC THIOETHERS

Author

BRZOZKA, Z, COBBEN, PLHM, REINHOUDT, DN, EDEMA, JJH, KELLOGG, RM, and Chemical Biology 2

Subjects
ION-SELECTIVE ELECTRODES, SILVER, THIA-CROWN ETHERS, COMPLEXATION, POTENTIOMETRY, CHEMFETS, ISFETS, PVC MEMBRANES, MACROCYCLIC THIOETHERS
Abstract

A chemically modified field-effect transistor (CHEMFET) with satisfactory Ag+ selectivity is described. The potentiometric Ag+ selectivities of CHEMFETs with plasticized PVC membranes based on macrocyclic thioethers have been determined. All the macrocyclic thioethers tested showed silver response and a selectivity towards Ag+ and Hg2+ versus other interfering cations. The highest Ag+ selectivity was obtained for a 14-membered cyclic tetrathioether with an exocyclic methylene group.

Published
1993

35. Ion selective transistor modelling for behavioural simulations.

Author

Daniel, M., Janicki, M., Wroblewski, W., Dybko, A., Brzozka, Z., and Napieralski, A.

Subjects
DETECTORS, HYDROGEN ions, FIELD-effect transistors, ENVIRONMENTAL monitoring, WATER quality management, HYDROLOGIC models
Abstract

Computer aided design and simulation of complex silicon microsystems oriented for environment monitoring requires efficient and accurate models of ion selective sensors, compatible with the existing behavioural simulators. This paper concerns sensors based on the back-side contact Ion Sensitive Field Effect Transistors (ISFETs). The ISFETs with silicon nitride gate are sensitive to hydrogen ion concentration. When the transistor gate is additionally covered with a special ion selective membrane, selectivity to other than hydrogen ions can be achieved. Such sensors are especially suitable for flow analysis of solutions containing various ions. The problem of ion selective sensor modelling is illustrated here on a practical example of an ammonium sensitive membrane. The membrane is investigated in the presence of some interfering ions and appropriate selectivity coefficients are determined. Then, the model of the whole sensor is created and used in subsequent electrical simulations. Providing that appropriate selectivity coefficients are known, the proposed model is applicable for any membrane, and can be straightforwardly implemented for behavioural simulation of water monitoring microsystems. The model has been already applied in a real on-line water pollution monitoring system for detection of various contaminants. [ABSTRACT FROM AUTHOR]

Published
1994

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