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3. The challenge of measuring sweet taste in food ingredients and products for regulatory compliance: A scientific opinion

Author

Starkey, Dustin E, primary, Wang, Zhuzhu, additional, Brunt, Kommer, additional, Dreyfuss, Lise, additional, Haselberger, Philip A, additional, Holroyd, Stephen E, additional, Janakiraman, Kaushik, additional, Kasturi, Prabhakar, additional, Konings, Erik J M, additional, Labbe, David, additional, Latulippe, Marie E, additional, Lavigne, Xavier, additional, McCleary, Barry V, additional, Parisi, Salvatore, additional, Shao, Tony, additional, Sullivan, Darryl, additional, Torres, Marina, additional, Yadlapalli, Sudhakar, additional, and Vrasidas, Ioannis, additional

Published
2022
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4. Dual-Laboratory Validation of a Method for the Determination of Fructans in Infant Formula and Adult Nutritionals: First Action 2016.14

Author

Brunt, Kommer, Sanders, Peter, Spichtig, Véronique, Ernste-Nota, Veronica, Sawicka, Paulina, Iwanoff, Kimberley, Van Soest, Jeroen, Lin, Paul Kong Thoo, Austin, Sean, Brunt, Kommer, Sanders, Peter, Spichtig, Véronique, Ernste-Nota, Veronica, Sawicka, Paulina, Iwanoff, Kimberley, Van Soest, Jeroen, Lin, Paul Kong Thoo, and Austin, Sean

Published
2021

11. Results Multi-Laboratory Trial ISO/CD 22184--IDF/WD 244: Milk and Milk Products--Determination of the Sugar Contents--High-Performance Anion Exchange Chromatography Method with Pulsed Amperometric Detection.

Author

Brunt, Kommer, Sanders, Peter, Ernste-Nota, Veronica, and van Soest, J.

Subjects
*DAIRY products, *LACTOSE, *INFANT formulas, *CHROMATOGRAPHIC analysis, *SUGAR, *MALTOSE, *GALACTOSE, *FRUCTOSE
Abstract

Background: In this international Multi-Laboratory Trial (MLT) the precision data and the accuracy of the ISO/CD 22184 IDF/WD 244 analytical method for the quantification of 6 different mono- and disaccharides (galactose, glucose, fructose, sucrose, lactose, and maltose) in milk and milk products with high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) are established. Objective: Thirteen different laboratories participated (located in New Zealand, the United States, and Europe). The study was divided into two parts. First, the learning/familiarization part in which the participants got experienced with the method. After successfully completing this part, the participants were allowed to continue with the second part of this trial: the analyses of the seven blind MLT duplicate samples. Method: The selected seven MLT samples comprise five MUVA reference dairy samples, one NIST-certified infant formula and one industrial sample. In the Tables S-1 to S-6 the results of this MLT study have been summarized per sugar. There is a very good agreement between the MUVA and NIST-certified sugar contents in the MLT samples and in the MLT established sugar contents in those MUVA and NIST samples. Results: This demonstrates that the accuracy of the newly developed HPAEC-PAD method is very good. Conclusions: Certificates of analysis were available for both the MUVA samples and the NIST sample. There is a very good agreement between the by MUVA- and NIS-certified sugar contents in the MLT samples and the corresponding in the MLT established sugar contents. This demonstrates that the accuracy of the HPAEC-PAD method is very good. In the IDF/ISO Analytical Week in June 2019 in Prague the report on the MLT study were discussed and adopted. [ABSTRACT FROM AUTHOR]

Published
2021
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23. Design and optimization of electrochemical detectors for HPLC and flow analysis systems

Author

Brunt, Kommer, Brunt, Kommer, Brunt, Kommer, and Brunt, Kommer

Abstract

In dit proefschrift worden twee nieuwe electrochemische detectoren beschreven. De differentiële amperometrische detector is speciaal ontworpen voor de hoge druk vloeistof chromatografie, terwijl de amperometrische detector die berust op het principe van een roterende " wall - jet " electrode ook zeer goed bruikbaar is in combinatie met continuous flow analysis " en "flow injection analysis" . ... Zie: Samenvatting

Published
1980

24. Dual-Laboratory Validation of a Method for the Determination of Fructans in Infant Formula and Adult Nutritionals: First Action 2016.14

Author

Brunt, Kommer, Sanders, Peter, Spichtig, Véronique, Ernste-Nota, Veronica, Sawicka, Paulina, Iwanoff, Kimberley, Van Soest, Jeroen, Lin, Paul Kong Thoo, Austin, Sean, Brunt, Kommer, Sanders, Peter, Spichtig, Véronique, Ernste-Nota, Veronica, Sawicka, Paulina, Iwanoff, Kimberley, Van Soest, Jeroen, Lin, Paul Kong Thoo, and Austin, Sean

Abstract

Until recently, only two AOAC Official MethodsSM have been available for the analysis of fructans: Method 997.08 and Method 999.03. Both are based on the analysis of the fructan component monosaccharides (glucose and fructose) after hydrolysis. The two methods have some limitations due to the strategies used for removing background interferences (such as from sucrose, α-glucooligosaccharides, and free sugars). The method described in this paper has been developed to overcome those limitations. The method is largely based on Method 999.03 and uses combined enzymatic and SPE steps to remove the interfering components without impacting the final analytical result. The method has been validated in two laboratories on infant formula and adult nutritionals. Recoveries were in the range of 86-119%, with most being in the range of 91-104%. RSDr values were in the range of 0.7-2.6%, with one exception when the fructan concentration was close to the LOQ, resulting in an RSDr of 8.9%. The performance is generallywithin the requirements outlined in the AOAC StandardMethod Performance Requirements (SMPR® 2014.002), which specifies recoveries in the range of 90-110% and RSDr values below 6%.

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