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13 results on '"Bruinsma, Lyon"'

9. Global transcriptional response of aspergillus niger to blocked active citrate export through deletion of the exporter gene

Author

Laothanachareon, Thanaporm, Bruinsma, Lyon, Nijsse, Bart, Schonewille, Tom, Suarez Diez, Maria, Tamayo Ramos, Juan Antonio, Santos, Vitor AP Martins dos, Schaap, Peter J., Laothanachareon, Thanaporm, Bruinsma, Lyon, Nijsse, Bart, Schonewille, Tom, Suarez Diez, Maria, Tamayo Ramos, Juan Antonio, Santos, Vitor AP Martins dos, and Schaap, Peter J.

Abstract

Aspergillus niger is the major industrial citrate producer worldwide. Export as well as uptake of citric acid are believed to occur by active, proton-dependent, symport systems. Both are major bottlenecks for industrial citrate production. Therefore, we assessed the consequences of deleting the citT gene encoding the A. niger citrate exporter, effectively blocking active citrate export. We followed the consumption of glucose and citrate as carbon sources, monitored the secretion of organic acids and carried out a thorough transcriptome pathway enrichment analysis. Under controlled cultivation conditions that normally promote citrate secretion, the knock-out strain secreted negligible amounts of citrate. Blocking active citrate export in this way led to a reduced glucose uptake and a reduced expression of high-affinity glucose transporter genes, mstG and mstH. The glyoxylate shunt was strongly activated and an increased expression of the OAH gene was observed, resulting in a more than two-fold higher concentration of oxalate in the medium. Deletion of citT did not affect citrate uptake suggesting that citrate export and citrate uptake are uncoupled from the system.

Published
2021

10. Global transcriptional response of aspergillus niger to blocked active citrate export through deletion of the exporter gene

Author

Laothanachareon, Thanaporn, Bruinsma, Lyon, Nijsse, Bart, Schonewille, Tom, Suarez-Diez, Maria, Tamayo-Ramos, Juan Antonio, Martins dos Santos, Vitor A.P., Schaap, Peter J., Laothanachareon, Thanaporn, Bruinsma, Lyon, Nijsse, Bart, Schonewille, Tom, Suarez-Diez, Maria, Tamayo-Ramos, Juan Antonio, Martins dos Santos, Vitor A.P., and Schaap, Peter J.

Abstract

Aspergillus niger is the major industrial citrate producer worldwide. Export as well as uptake of citric acid are believed to occur by active, proton-dependent, symport systems. Both are major bottlenecks for industrial citrate production. Therefore, we assessed the consequences of deleting the citT gene encoding the A. niger citrate exporter, effectively blocking active citrate export. We followed the consumption of glucose and citrate as carbon sources, monitored the secretion of organic acids and carried out a thorough transcriptome pathway enrichment analysis. Under controlled cultivation conditions that normally promote citrate secretion, the knock-out strain secreted negligible amounts of citrate. Blocking active citrate export in this way led to a reduced glucose uptake and a reduced expression of high-affinity glucose transporter genes, mstG and mstH. The glyoxylate shunt was strongly activated and an increased expression of the OAH gene was observed, resulting in a more than two-fold higher concentration of oxalate in the medium. Deletion of citT did not affect citrate uptake suggesting that citrate export and citrate uptake are uncoupled from the system.

Published
2021

11. Expression of the Alternative Oxidase Influences JNK Signaling and Cell Migration

Author

Andjelković, Ana, Mordas, Amelia, Bruinsma, Lyon, Ketola, Annika, Cannino, Giuseppe, Giordano, Luca, Dhandapani, Praveen K, Szibor, Marten, Dufour, Eric, Jacobs, Howard T, Lääketieteen ja biotieteiden tiedekunta - Faculty of Medicine and Life Sciences, and University of Tampere

Subjects
Biokemia, solu- ja molekyylibiologia - Biochemistry, cell and molecular biology
Published
2018

12. Expression of the alternative oxidase influences jun n-terminal kinase signaling and cell migration

Author

Andjelkovic, Ana, Mordas, Amelia, Bruinsma, Lyon, Ketola, Annika, Cannino, Giuseppe, Giordano, Luca, Dhandapani, Praveen K., Szibor, Marten, Dufour, Eric, Jacobs, Howard T., and Institute of Biotechnology

Subjects
Systeem en Synthetische Biologie, HUMAN-PAPILLOMAVIRUS TYPE-16, C-JUN, Alternative oxidase, DISC EVERSION, Wound healing, JNK PATHWAY, AP-1, DROSOPHILA, Jun N-terminal kinase, 1182 Biochemistry, cell and molecular biology, Systems and Synthetic Biology, TRANSCRIPTION FACTOR, THORAX CLOSURE, Transcription, GENE-EXPRESSION, LIFE-SPAN
Abstract

Downregulation of Jun N-terminal kinase (JNK) signaling inhibits cell migration in diverse model systems. In Drosophila pupal development, attenuated JNK signaling in the thoracic dorsal epithelium leads to defective midline closure, resulting in cleft thorax. Here we report that concomitant expression of the Ciona intestinalis alternative oxidase (AOX) was able to compensate for JNK pathway downregulation, substantially correcting the cleft thorax phenotype. AOX expression also promoted wound-healing behavior and single-cell migration in immortalized mouse embryonic fibroblasts (iMEFs), counteracting the effect of JNK pathway inhibition. However, AOX was not able to rescue developmental phenotypes resulting from knockdown of the AP-1 transcription factor, the canonical target of JNK, nor its targets and had no effect on AP-1-dependent transcription. The migration of AOX-expressing iMEFs in the wound-healing assay was differentially stimulated by antimycin A, which redirects respiratory electron flow through AOX, altering the balance between mitochondrial ATP and heat production. Since other treatments affecting mitochondrial ATP did not stimulate wound healing, we propose increased mitochondrial heat production as the most likely primary mechanism of action of AOX in promoting cell migration in these various contexts.

Published
2018
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