Search

BackgroundSkin-penetrating nematodes of the genus Strongyloides infect over 600 million people, posing a major global health burden. Their life cycle includes both a parasitic and free-living generation. During the parasitic generation, infective third-stage larvae (iL3s) actively engage in host seeking. During the free-living generation, the nematodes develop and reproduce on host feces. At different points during their life cycle, Strongyloides species encounter a wide variety of host-associated and environmental bacteria. However, the microbiome associated with Strongyloides species, and the behavioral and physiological interactions between Strongyloides species and bacteria, remain unclear.ResultsWe first investigated the microbiome of the human parasite Strongyloides stercoralis using 16S-based amplicon sequencing. We found that S. stercoralis free-living adults have an associated microbiome consisting of specific fecal bacteria. We then investigated the behavioral responses of S. stercoralis and the closely related rat parasite Strongyloides ratti to an ecologically diverse panel of bacteria. We found that S. stercoralis and S. ratti showed similar responses to bacteria. The responses of both nematodes to bacteria varied dramatically across life stages: free-living adults were strongly attracted to most of the bacteria tested, while iL3s were attracted specifically to a narrow range of environmental bacteria. The behavioral responses to bacteria were dynamic, consisting of distinct short- and long-term behaviors. Finally, a comparison of the growth and reproduction of S. stercoralis free-living adults on different bacteria revealed that the bacterium Proteus mirabilis inhibits S. stercoralis egg hatching, and thereby greatly decreases parasite viability.ConclusionsSkin-penetrating nematodes encounter bacteria from various ecological niches throughout their life cycle. Our results demonstrate that bacteria function as key chemosensory cues for directing parasite movement in a life-stage-specific manner. Some bacterial genera may form essential associations with the nematodes, while others are detrimental and serve as a potential source of novel nematicides.

Approximately 800 million people worldwide are infected with one or more species of skin-penetrating nematodes. These parasites persist in the environment as developmentally arrested third-stage infective larvae (iL3s) that navigate toward host-emitted cues, contact host skin, and penetrate the skin. iL3s then reinitiate development inside the host in response to sensory cues, a process called activation. Here, we investigate how chemosensation drives host seeking and activation in skin-penetrating nematodes. We show that the olfactory preferences of iL3s are categorically different from those of free-living adults, which may restrict host seeking to iL3s. The human-parasitic threadworm Strongyloides stercoralis and hookworm Ancylostoma ceylanicum have highly dissimilar olfactory preferences, suggesting that these two species may use distinct strategies to target humans. CRISPR/Cas9-mediated mutagenesis of the S. stercoralis tax-4 gene abolishes iL3 attraction to a host-emitted odorant and prevents activation. Our results suggest an important role for chemosensation in iL3 host seeking and infectivity and provide insight into the molecular mechanisms that underlie these processes.

Bird species that undertake irruptive migrations are good candidates for assessing density-dependent effects on stopover ecology because the number of birds using stopover sites varies considerably from year to year. Using morphometric data from a 9-year banding program and radiotelemetry (n  = 25 females), we found that increases in the annual density of Northern Saw-whet Owls (NSWO; Aegolius acadicus) encountered at a fall stopover site in Nova Scotia, Canada, were not linked to female body condition or minimum stopover duration. Rather, most NSWO spent no more than 1 or 2 full days at the stopover site following radio-tagging and during their return visits to the site. Body condition indices were highest for NSWO captured near the end of the migration monitoring season, possibly reflecting birds that had recently established wintering ranges and were elevating energy stores. We used breeding abundance indices derived from Birds Canada's Atlantic Nocturnal Owl Survey to help test the hypothesis that irruptive fall migrations in NSWO were driven by elevated breeding productivity and dispersal of immature birds (breeding success hypothesis). Indeed, irruptive fall migrations were characterized by elevated densities of immatures, but not adults, and mean breeding abundance indices for the Maritime provinces during years with irruptive migrations were higher than those for non-irruptive years. We hypothesize that prey abundance during years with irruptive migrations was sufficient to enable high breeding densities and minimize effects of elevated fall densities of NSWO on rates of mass gain and stopover duration. [ABSTRACT FROM AUTHOR]