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The ability of cells to maintain their genomic integrity and accurately repair any DNA damage is crucial to their continued survival and functioning. One such type of damage, double-stranded breaks, is primarily addressed in Escherichia coli (E. coli) by RecA-mediated homologous recombination, a highly conserved pathway in which the damaged region is matched to a homology, which is then used as a template to bridge the gap. This strand exchange event forms a secondary structure known as a Holliday junction, which must be resolved before the gap can be fully repaired. Although the broad outline of this pathway is well understood, there are still many details that require further investigation. In this study, we examined two proteins, RadA and RadD, which have both been broadly implicated in double-stranded break repair. Previous results have shown shared functions and shared mutant phenotypes, but have not conclusively shown that they physically interact. To that end, we used a yeast two-hybrid assay, which showed an interaction, followed by further yeast two-hybrids in order to examine whether specific RadA mutants could disrupt this interaction.

Are you in the process of writing a good assignment or dissertation that will help you get good marks or applause? Well, the best tip that you need today is to not write a regular bibliography and instead go for the annotated bibliography. If you have been someone who always asked friends and parents questions like to help me with essays andwrite my essay, you may be in a messy situation right now. But, in this article, we will provide you with tips to write a perfect annotated bibliography. Thus, instead of asking your friends, “help me with essays,” you can now give them advice about how to write a perfect annotated bibliography. Follow these tips For Perfect Annotated Bibliography: Summarize the source The first towards writing an annotated bibliography is to summarize the sources you have used while completing the assignment. You can summarize your sources easily by taking down notes or by pointing out major highlights of the sources. Citations Citations are a necessity when someone asks you to “write my essay” or bibliography. It basically means references that are taken from books and other scholarly sources. These are the references that will help you to support your argument. Annotated bibliography The best part about an annotated bibliography is that it is easy to read, and it also helps the readers to know what sources they are going to check for different references. Thus, if you want to make the task easier for the reader, writing annotated bibliography can be a good idea to follow. List of the central theme It is also important to make a list of central themes that focuses on your writing. The list of central themes will give a brief overview of the reader about your writing and the goal and idea behind the writing piece you submitted. In addition to this, you have to explain to your reader why you have written certain points and why you have used a particular reference. Thus, the list of central themes will help your reader to understand the topic in a better way. Evaluate sources It is very important to make a critical evaluation of each source that you have used in your writing. If there are any tests and devices used, you need to include the list in your writing. Let the reader know if you have added value to the research work or not. In addition to this, also add the chronology of the book used. It is important to write if the book you have used is current or outdated. Conclusion An annotated bibliography will help you to become a better writer and researcher. Thus, the next time when you have to complete research and writing on time, you want to ask your friends and family members questions like “Help me with an assignment.” you will know all details, descriptions, and tips needed to write a good quality essay. Finding good sources and writing a research paper takes time. But, if you follow the annotated bibliography, you will be able to convince the reader about your research work. Original Source:https://www.valdezlink.com/perfect-annotated-bibliography