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24 results on '"Briolat, Valérie"'

5. RNase R is associated in a functional complex with the RhpA DEAD-box RNA helicase inHelicobacter pylori

Author

Tejada-Arranz, Alejandro, primary, Matos, Rute G, additional, Quentin, Yves, additional, Bouilloux-Lafont, Maxime, additional, Galtier, Eloïse, additional, Briolat, Valérie, additional, Kornobis, Etienne, additional, Douché, Thibaut, additional, Matondo, Mariette, additional, Arraiano, Cecilia M, additional, Raynal, Bertrand, additional, and De Reuse, Hilde, additional

Published
2021
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7. Mining zebrafish microbiota reveals key community-level resistance against fish pathogen infection

Author

Stressmann, Franziska A., primary, Bernal-Bayard, Joaquín, additional, Perez-Pascual, David, additional, Audrain, Bianca, additional, Rendueles, Olaya, additional, Briolat, Valérie, additional, Bruchmann, Sebastian, additional, Volant, Stevenn, additional, Ghozlane, Amine, additional, Häussler, Susanne, additional, Duchaud, Eric, additional, Levraud, Jean-Pierre, additional, and Ghigo, Jean-Marc, additional

Published
2020
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11. A large new subset of TRIM genes highly diversified by duplication and positive selection in teleost fish

Author

Herbomel Philippe, Lauret Emilie, Briolat Valérie, Yahmi Malika, Levraud Jean-Pierre, van der Aa Lieke M, Benmansour Abdenour, and Boudinot Pierre

Subjects
Biology (General), QH301-705.5
Abstract

Abstract Background In mammals, the members of the tripartite motif (TRIM) protein family are involved in various cellular processes including innate immunity against viral infection. Viruses exert strong selective pressures on the defense system. Accordingly, antiviral TRIMs have diversified highly through gene expansion, positive selection and alternative splicing. Characterizing immune TRIMs in other vertebrates may enlighten their complex evolution. Results We describe here a large new subfamily of TRIMs in teleosts, called finTRIMs, identified in rainbow trout as virus-induced transcripts. FinTRIMs are formed of nearly identical RING/B-box regions and C-termini of variable length; the long variants include a B30.2 domain. The zebrafish genome harbors a striking diversity of finTRIMs, with 84 genes distributed in clusters on different chromosomes. A phylogenetic analysis revealed different subsets suggesting lineage-specific diversification events. Accordingly, the number of fintrim genes varies greatly among fish species. Conserved syntenies were observed only for the oldest fintrims. The closest mammalian relatives are trim16 and trim25, but they are not true orthologs. The B30.2 domain of zebrafish finTRIMs evolved under strong positive selection. The positions under positive selection are remarkably congruent in finTRIMs and in mammalian antiviral TRIM5α, concentrated within a viral recognition motif in mammals. The B30.2 domains most closely related to finTRIM are found among NOD-like receptors (NLR), indicating that the evolution of TRIMs and NLRs was intertwined by exon shuffling. Conclusion The diversity, evolution, and features of finTRIMs suggest an important role in fish innate immunity; this would make them the first TRIMs involved in immunity identified outside mammals.

Published
2009
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12. Imaging of viral neuroinvasion in the zebrafish reveals that Sindbis and chikungunya viruses favour different entry routes

Author

Passoni, Gabriella, Langevin, Christelle, Palha, Nuno, Mounce, Bryan C., Briolat, Valérie, Affaticati, Pierre, De Job, Elodie, Joly, Jean-Stéphane, Vignuzzi, Marco, Saleh, Maria-Carla, Herbomel, Philippe, Boudinot, Pierre, Levraud, Jean-Pierre, Unité de recherche Virologie et Immunologie Moléculaires (VIM), Institut National de la Recherche Agronomique (INRA), Macrophages et Développement de l’Immunité, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Populations virales et Pathogenèse, Institut des Neurosciences de Paris-Saclay (Neuro-PSI), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Virus et Interférence ARN, Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], This work was supported by funding from the European Community's Seventh Framework Programme [FP7-PEOPLE-2011-ITN] under grant agreement no. PITN-GA-2011-289209 for the Marie Curie Initial Training Network FishForPharma, the Agence Nationale de la Recherche (Zebraflam grant ANR-10-MDI-009), a Domaine d'Interet Majeur Maladies Infectieuses (DIM-Malinf) grant from Région Ile-de-France, institutional support was from Institut National de la Recherche Agronomique (INRA), the Institut Pasteur and Centre National de la Recherche Scientifique. This work also benefited from the facilities and expertise of TEFOR – Investissement d'avenir – ANR-II-INBS-0014., ANR-10-MIDI-0009,ZebraFlam,Signaux et cellules de la réponse inflammatoire: suivi en temps réel chez un vertébré entier, le danio zébré(2010), European Project: 289209,EC:FP7:PEOPLE,FP7-PEOPLE-2011-ITN,FISHFORPHARMA(2012), Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)), Populations virales et Pathogenèse - Viral Populations and Pathogenesis, Institut des Neurosciences Paris-Saclay (NeuroPSI), Virus et Interférence ARN - Viruses and RNA Interference, and Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)

Subjects
lcsh:Medicine, poisson zèbre, virus, Alphavirus, Virus Replication, Axonal Transport, Nervous System, Tropism, Imaging, Three-Dimensional, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, lcsh:Pathology, Animals, Zebrafish, Live imaging, Alphavirus Infections, Macrophages, lcsh:R, Endothelial Cells, virus diseases, Virus Internalization, Viral encephalitis, Zebra, Blood-Brain Barrier, Central nervous system, Larva, invasion cellulaire, Microvessels, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Chikungunya Fever, Chikungunya, Sindbis Virus, Chikungunya virus, Research Article, lcsh:RB1-214
Abstract

Alphaviruses, such as chikungunya virus (CHIKV) and Sindbis virus (SINV), are vector-borne pathogens that cause acute illnesses in humans and are sometimes associated with neuropathies, especially in infants and elderly patients. Little is known about their mechanism of entry into the central nervous system (CNS), even for SINV, which has been used extensively as a model for viral encephalopathies. We previously established a CHIKV infection model in the optically transparent zebrafish larva; here we describe a new SINV infection model in this host. We imaged in vivo the onset and progression of the infection caused by intravenous SINV inoculation. Similar to that described for CHIKV, infection in the periphery was detected early and was transient, whereas CNS infection started at later time points and was persistent or progressive. We then tested the possible mechanisms of neuroinvasion by CHIKV and SINV. Neither virus relied on macrophage-mediated transport to access the CNS. CHIKV, but not SINV, always infects endothelial cells of the brain vasculature. By contrast, axonal transport was much more efficient with SINV than CHIKV, both from the periphery to the CNS and between neural tissues. Thus, the preferred mechanisms of neuroinvasion by these two related viruses are distinct, providing a powerful imaging-friendly system to compare mechanisms and prevention methods of encephalopathies., Summary: Imaging of neuroinvasion in zebrafish shows that chikungunya virus first infects the blood-brain barrier, whereas Sindbis virus relies on axonal transport.

Published
2017

14. Mécanismes d’inhibition de la réplication de rhabdovirus de poissons par la protéine ubiquitin like ISG15 de poisson zèbre

Author

Langevin, Christelle, Van der Aa, Lieke, Houel, Armel, Torhy, Corinne, Briolat, Valérie, Lunazzi, Aurelie, Harmache, Abdallah, Bremont-Deletang, Michel, Levraud, Jean-Pierre, Boudinot, Pierre, Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)), Institut National de la Recherche Agronomique (INRA), Institut Pasteur [Paris], Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), and Institut National de la Recherche Agronomique (INRA)-Université de Tours

Subjects
[SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2012

17. Real-Time Whole-Body Visualization of Chikungunya Virus Infection and Host Interferon Response in Zebrafish

Author

Palha, Nuno, primary, Guivel-Benhassine, Florence, additional, Briolat, Valérie, additional, Lutfalla, Georges, additional, Sourisseau, Marion, additional, Ellett, Felix, additional, Wang, Chieh-Huei, additional, Lieschke, Graham J., additional, Herbomel, Philippe, additional, Schwartz, Olivier, additional, and Levraud, Jean-Pierre, additional

Published
2013
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23. Characterization of samhd1 Morphant Zebrafish Recapitulates Features of the Human Type I Interferonopathy Aicardi-Goutières Syndrome

Author

Yanick J. Crow, Catherine Morrissey, Leo A. H. Zeef, Emma M. Jenkinson, Gillian I. Rice, Valérie Briolat, Jean-Pierre Levraud, David Gent, Paul R. Kasher, Manchester Centre for Genomic Medicine (MCGM), Manchester Academic Health Science Centre (MAHSC), University of Manchester [Manchester]-University of Manchester [Manchester]-Faculty of Biology, Medicine and Health [Manchester, UK], University of Manchester [Manchester]-Manchester University NHS Foundation Trust (MFT)-St Mary's Hospital Manchester, Macrophages et Développement de l'Immunité, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Faculty of Life Sciences [Manchester], University of Manchester [Manchester], Laboratory of neurogenetics and neuroinflammation (Equipe Inserm U1163), Imagine - Institut des maladies génétiques (IHU) (Imagine - U1163), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP), This work was supported by the Newlife Foundation, the Natalie Kate Moss Trust, the European Research Council (GA 309449: fellowship to Y.J.C.), and state subsidies managed by the National Research Agency (France) under the 'Investments for the Future' program bearing the reference ANR-10-IAHU-01 and the 'Zebraflam' program bearing the reference ANR-10-MIDI-009., We thank Dr. Adam Hurlstone and Dr. Shane Herbert for sharing zebrafish strains and equipment and Mike Jackson at the University of Manchester Flow Cytometry Facility for technical support., ANR-10-MIDI-0009,ZebraFlam,Signaux et cellules de la réponse inflammatoire: suivi en temps réel chez un vertébré entier, le danio zébré(2010), Manchester Centre for Genomic Medicine [Manchester, UK] (MCGM), St Mary's Hospital Manchester-Manchester Academic Health Science Centre (MAHSC), University of Manchester [Manchester]-University of Manchester [Manchester]-Manchester University NHS Foundation Trust (MFT)-Faculty of Biology, Medicine and Health [Manchester, UK], Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Briolat, Valérie, and MECANISMES INTEGRES DE L'INFLAMMATION - Signaux et cellules de la réponse inflammatoire: suivi en temps réel chez un vertébré entier, le danio zébré - - ZebraFlam2010 - ANR-10-MIDI-0009 - MI2 - VALID

Subjects
MESH: Interferon Type I, Adenosine Deaminase, MESH: Sequence Homology, Amino Acid, [SDV]Life Sciences [q-bio], ved/biology.organism_classification_rank.species, MESH: Microscopy, Fluorescence, MESH: Amino Acid Sequence, MESH: Acid Anhydride Hydrolases, [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity, Cerebral Ventricles, Animals, Genetically Modified, MESH: Reverse Transcriptase Polymerase Chain Reaction, MESH: Gene Expression Regulation, Developmental, Immunology and Allergy, MESH: Animals, MESH: Interferons, Zebrafish, MESH: Adenosine Deaminase, Gene knockdown, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Regulation, Developmental, MESH: Rhombencephalon, MESH: SAM Domain and HD Domain-Containing Protein 1, Acid Anhydride Hydrolases, 3. Good health, Cell biology, [SDV] Life Sciences [q-bio], Gene Knockdown Techniques, Interferon Type I, [SDV.IMM]Life Sciences [q-bio]/Immunology, MESH: Immunity, Innate, Intracranial Hemorrhages, MESH: Intracranial Hemorrhages, [SDV.IMM] Life Sciences [q-bio]/Immunology, Blotting, Western, Molecular Sequence Data, Immunology, MESH: Zebrafish Proteins, Biology, Nervous System Malformations, MESH: Nervous System Malformations, SAM Domain and HD Domain-Containing Protein 1, MESH: Animals, Genetically Modified, Autoimmune Diseases of the Nervous System, Immune system, medicine, Animals, Humans, MESH: Blotting, Western, Amino Acid Sequence, Model organism, MESH: Zebrafish, [SDV.IMM.II] Life Sciences [q-bio]/Immunology/Innate immunity, Loss function, Innate immune system, MESH: Humans, MESH: Molecular Sequence Data, Sequence Homology, Amino Acid, ved/biology, Morphant, Zebrafish Proteins, medicine.disease, biology.organism_classification, MESH: Gene Knockdown Techniques, Immunity, Innate, MESH: Autoimmune Diseases of the Nervous System, Rhombencephalon, Disease Models, Animal, Microscopy, Fluorescence, Aicardi–Goutières syndrome, MESH: Cerebral Ventricles, Interferons, MESH: Disease Models, Animal
Abstract

In humans, loss of function mutations in the SAMHD1 (AGS5) gene cause a severe form of Aicardi-Goutières syndrome (AGS), an inherited inflammatory-mediated encephalopathy characterized by increased type I IFN activity and upregulation of IFN-stimulated genes (ISGs). In particular, SAMHD1-related AGS is associated with a distinctive cerebrovascular pathology that commonly leads to stroke. Although inflammatory responses are observed in immune cells cultured from Samhd1 null mouse models, these mice are physically healthy, specifically lacking a brain phenotype. We have investigated the use of zebrafish as an alternative system for generating a clinically relevant model of SAMHD1-related AGS. Using temporal gene knockdown of zebrafish samhd1, we observe hindbrain ventricular swelling and brain hemorrhage. Furthermore, loss of samhd1 or of another AGS-associated gene, adar, leads to a significant upregulation of innate immune-related genes and an increase in the number of cells expressing the zebrafish type I IFN ifnphi1. To our knowledge, this is the first example of an in vivo model of AGS that recapitulates features of both the innate immune and neurological characteristics of the disease. The phenotypes associated with loss of samhd1 and adar suggest a function of these genes in controlling innate immune processes conserved to zebrafish, thereby also contributing to our understanding of antiviral signaling in this model organism.

Published
2015

24. Imaging of viral neuroinvasion in the zebrafish reveals that Sindbis and chikungunya viruses favour different entry routes.

Author

Passoni G, Langevin C, Palha N, Mounce BC, Briolat V, Affaticati P, De Job E, Joly JS, Vignuzzi M, Saleh MC, Herbomel P, Boudinot P, and Levraud JP

Subjects
Alphavirus Infections pathology, Alphavirus Infections virology, Animals, Axonal Transport, Blood-Brain Barrier pathology, Blood-Brain Barrier virology, Chikungunya Fever pathology, Chikungunya Fever virology, Endothelial Cells pathology, Endothelial Cells virology, Larva virology, Macrophages metabolism, Microvessels pathology, Nervous System pathology, Tropism physiology, Virus Replication physiology, Zebrafish, Chikungunya virus physiology, Imaging, Three-Dimensional, Nervous System virology, Sindbis Virus physiology, Virus Internalization
Abstract

Alphaviruses, such as chikungunya virus (CHIKV) and Sindbis virus (SINV), are vector-borne pathogens that cause acute illnesses in humans and are sometimes associated with neuropathies, especially in infants and elderly patients. Little is known about their mechanism of entry into the central nervous system (CNS), even for SINV, which has been used extensively as a model for viral encephalopathies. We previously established a CHIKV infection model in the optically transparent zebrafish larva; here we describe a new SINV infection model in this host. We imaged in vivo the onset and progression of the infection caused by intravenous SINV inoculation. Similar to that described for CHIKV, infection in the periphery was detected early and was transient, whereas CNS infection started at later time points and was persistent or progressive. We then tested the possible mechanisms of neuroinvasion by CHIKV and SINV. Neither virus relied on macrophage-mediated transport to access the CNS. CHIKV, but not SINV, always infects endothelial cells of the brain vasculature. By contrast, axonal transport was much more efficient with SINV than CHIKV, both from the periphery to the CNS and between neural tissues. Thus, the preferred mechanisms of neuroinvasion by these two related viruses are distinct, providing a powerful imaging-friendly system to compare mechanisms and prevention methods of encephalopathies., Competing Interests: Competing interestsThe authors declare no competing or financial interests., (© 2017. Published by The Company of Biologists Ltd.)

Published
2017
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