Search

Your search keyword '"Brett, Paul J"' showing total 238 results
Start Over Author "Brett, Paul J"
238 results on '"Brett, Paul J"'

3. Identification of Burkholderia cepacia strains that express a Burkholderia pseudomallei -like capsular polysaccharide

Author

Burtnick, Mary N., primary, Dance, David A. B., additional, Vongsouvath, Manivanh, additional, Newton, Paul N., additional, Dittrich, Sabine, additional, Sendouangphachanh, Amphone, additional, Woods, Kate, additional, Davong, Viengmon, additional, Kenna, Dervla T. D., additional, Saiprom, Natnaree, additional, Sengyee, Sineenart, additional, Hantrakun, Viriya, additional, Wuthiekanun, Vanaporn, additional, Limmathurotsakul, Direk, additional, Chantratita, Narisara, additional, and Brett, Paul J., additional

Published
2024
Full Text
View/download PDF

4. Identification of Circulating Bacterial Antigens by In Vivo Microbial Antigen Discovery

Author

Nuti, Dana E, Crump, Reva B, Handayani, Farida Dwi, Chantratita, Narisara, Peacock, Sharon J, Bowen, Richard, Felgner, Philip L, Davies, D. Huw, Wu, Terry, Lyons, C. Rick, Brett, Paul J, Burtnick, Mary N, Kozel, Thomas R, and AuCoin, David P

Subjects
burkholderia pseudomonas pseudomallei, francisella-tularensis, subtractive hybridization, capsular polysaccharide, invasive aspergillosis, monoclonal-antibodies, virulence determinant, structural-analysis, o-antigen, melioidosis
Abstract

Detection of microbial antigens in clinical samples can lead to rapid diagnosis of an infection and administration of appropriate therapeutics. A major barrier in diagnostics development is determining which of the potentially hundreds or thousands of antigens produced by a microbe are actually present in patient samples in detectable amounts against a background of innumerable host proteins. In this report, we describe a strategy, termed in vivo microbial antigen discovery (InMAD), that we used to identify circulating bacterial antigens. This technique starts with "InMAD serum," which is filtered serum that has been harvested from BALB/c mice infected with a bacterial pathogen. The InMAD serum, which is free of whole bacterial cells, is used to immunize syngeneic BALB/c mice. The resulting " InMAD immune serum" contains antibodies specific for the soluble microbial antigens present in sera from the infected mice. The InMAD immune serum is then used to probe blots of bacterial lysates or bacterial proteome arrays. Bacterial antigens that are reactive with the InMAD immune serum are precisely the antigens to target in an antigen immunoassay. By employing InMAD, we identified multiple circulating antigens that are secreted or shed during infection using Burkholderia pseudomallei and Francisella tularensis as model organisms. Potential diagnostic targets identified by the InMAD approach included bacterial proteins, capsular polysaccharide, and lipopolysaccharide. The InMAD technique makes no assumptions other than immunogenicity and has the potential to be a broad discovery platform to identify diagnostic targets from microbial pathogens. IMPORTANCE Effective treatment of microbial infection is critically dependent on early diagnosis and identification of the etiological agent. One means for rapid diagnosis is immunoassay for antigens that are shed into body fluids during infection. Immunoassays can be inexpensive, rapid, and adaptable to a point-of-care format. A major impediment to immunoassay for diagnosis of infectious disease is identification of appropriate antigen targets. This report describes a strategy that can be used for identification of microbial antigens that are shed into serum during infection by the biothreats Burkholderia pseudomallei and Francisella tularensis. Termed InMAD (in vivo microbial antigen discovery), the strategy has the potential for application to a broad spectrum of microbial pathogens.

Published
2011

5. Prospective Analysis of Antibody Diagnostic Tests and TTS1 Real-Time PCR for Diagnosis of Melioidosis in Areas Where It Is Endemic

Author

Noparatvarakorn, Chawitar, primary, Sengyee, Sineenart, additional, Yarasai, Atchara, additional, Phunpang, Rungnapa, additional, Dulsuk, Adul, additional, Ottiwet, Orawan, additional, Janon, Rachan, additional, Morakot, Chumpol, additional, Burtnick, Mary N., additional, Brett, Paul J., additional, West, T. Eoin, additional, and Chantratita, Narisara, additional

Published
2023
Full Text
View/download PDF

9. Layered and integrated medical countermeasures against Burkholderia pseudomallei infections in C57BL/6 mice

Author

Klimko, Christopher P., primary, Shoe, Jennifer L., additional, Rill, Nathaniel O., additional, Hunter, Melissa, additional, Dankmeyer, Jennifer L., additional, Talyansky, Yuli, additional, Schmidt, Lindsey K., additional, Orne, Caitlyn E., additional, Fetterer, David P., additional, Biryukov, Sergei S., additional, Burtnick, Mary N., additional, Brett, Paul J., additional, DeShazer, David, additional, and Cote, Christopher K., additional

Published
2022
Full Text
View/download PDF

10. Evaluation of two different vaccine platforms for immunization against melioidosis and glanders

Author

Biryukov, Sergei S., primary, Cote, Christopher K., additional, Klimko, Christopher P., additional, Dankmeyer, Jennifer L., additional, Rill, Nathaniel O., additional, Shoe, Jennifer L., additional, Hunter, Melissa, additional, Shamsuddin, Zain, additional, Velez, Ivan, additional, Hedrick, Zander M., additional, Rosario-Acevedo, Raysa, additional, Talyansky, Yuli, additional, Schmidt, Lindsey K., additional, Orne, Caitlyn E., additional, Fetterer, David P., additional, Burtnick, Mary N., additional, Brett, Paul J., additional, Welkos, Susan L., additional, and DeShazer, David, additional

Published
2022
Full Text
View/download PDF

11. Evaluation of antigen-detecting and antibody-detecting diagnostic test combinations for diagnosing melioidosis

Author

Amornchai, Premjit, primary, Hantrakun, Viriya, additional, Wongsuvan, Gumphol, additional, Wuthiekanun, Vanaporn, additional, Wongratanacheewin, Surasakdi, additional, Teparrakkul, Prapit, additional, West, T. Eoin, additional, AuCoin, David P., additional, Day, Nicholas P. J., additional, Brett, Paul J., additional, Burtnick, Mary N., additional, Chantratitra, Narisara, additional, and Limmathurotsakul, Direk, additional

Published
2021
Full Text
View/download PDF

15. Molecular and physical characterization of Burkholderia mallei O antigens

Author

Burtnick, Mary N., Brett, Paul J., and Woods, Donald E.

Subjects
Endotoxins -- Genetic aspects, Gram-negative bacteria -- Genetic aspects, Gene expression -- Analysis, Biological sciences
Abstract

Research points out that the O polysaccharide moiety of Burkholderia mallei lipopolysaccharide mediates the resistance to the bactericidal action of serum. The gene cluster of O polysaccharide biosynthesis and its structure are discussed.

Published
2002

16. Molecular characterization of genetic loci required for secretion of exoproducts in Burkholderia pseudomallei

Author

DeShazer, David, Brett, Paul J., Burtnick, Mary N., and Woods, Donald E.

Subjects
Gram-negative bacteria -- Genetic aspects, Microbial enzymes -- Genetic aspects, Biological sciences
Abstract

Previous investigations have shown that Burkholderia pseudomallei secretes protease, lipase and phospholipase C into the extracellular milieu, but their mechanisms of secretion and roles in pathogenesis were not clear. B. pseduomallei 1026b transposon mutants deficient in secretion of protease, lipase and phospholipase C have been isolated and characterized. The relative virulence of 1026b and DD213, a representative secretion mutant, in the Syrian hamster model of melioidosis, were compared. Findings showed that exoproducts secreted by the type II pathway may serve a minor role in B. pseudomallei pathogenesis.

Published
1999

20. Mutagenesis of Burkholderia pseudomallei with Tn5-OT182: isolation of motility mutants and molecular characterization of the flagellin structural gene

Author

DeShazer, David, Brett, Paul J., Carlyon, Robert, and Woods, Donald E.

Subjects
Virulence (Microbiology) -- Genetic aspects, Gram-negative bacteria -- Genetic aspects, Microorganisms -- Motility, Flagella (Microbiology) -- Genetic aspects, Biological sciences
Abstract

The mutagenesis of Burkholderia pseudomallei with Tn5-OT182 was analyzed to identify the multiple genes that are involved in motility including the flagellin structural gene, fliC. Genetic analysis of the gram-negative bacillus via transposon mutagenesis with Tn5-OT182 indicated the presence of unique motility mutants that contained Tn5-OT182 integrations in a variety of genes encoding chemotaxis and flagellar proteins. However, there were no significant differences in the virulence of fliC-mutants and wild-type Burkholderia pseudomallei.

Published
1997

21. Caprine humoral response to Burkholderia pseudomallei antigens during acute melioidosis from aerosol exposure

Author

Yi, Jinhee, primary, Simpanya, Mukoma F., additional, Settles, Erik W., additional, Shannon, Austin B., additional, Hernandez, Karen, additional, Pristo, Lauren, additional, Keener, Mitchell E., additional, Hornstra, Heidie, additional, Busch, Joseph D., additional, Soffler, Carl, additional, Brett, Paul J., additional, Currie, Bart J., additional, Bowen, Richard A., additional, Tuanyok, Apichai, additional, and Keim, Paul, additional

Published
2019
Full Text
View/download PDF

22. Melioidosis patient serum-reactive synthetic tetrasaccharides bearing the predominant epitopes ofBurkholderia pseudomalleiandBurkholderia malleiO-antigens

Author

Cloutier, Maude, primary, Delar, Emmanilo, additional, Muru, Kevin, additional, Ndong, Seynabou, additional, Hoyeck, Robert R., additional, Kaewarpai, Taniya, additional, Chantratita, Narisara, additional, Burtnick, Mary N., additional, Brett, Paul J., additional, and Gauthier, Charles, additional

Published
2019
Full Text
View/download PDF

25. Caprine humoral response to Burkholderia pseudomallei antigens during acute melioidosis from aerosol exposure

Author

Yi, Jinhee, primary, Simpanya, Mukoma F., additional, Settles, Erik W., additional, Shannon, Austin B., additional, Hernandez, Karen, additional, Pristo, Lauren, additional, Keener, Mitchell E., additional, Hornstra, Heidie, additional, Busch, Joseph D., additional, Soffler, Carl, additional, Brett, Paul J., additional, Currie, Bart J., additional, Bowen, Richard A., additional, Tuanyok, Apichai, additional, and Keim, Paul, additional

Published
2018
Full Text
View/download PDF

26. A Rapid Immunochromatography Test Based on Hcp1 Is a Potential Point-of-Care Test for Serological Diagnosis of Melioidosis

Author

Phokrai, Phornpun, primary, Karoonboonyanan, Wisansanee, additional, Thanapattarapairoj, Nida, additional, Promkong, Chidchanok, additional, Dulsuk, Adul, additional, Koosakulnirand, Sirikamon, additional, Canovali, Sasha, additional, Indrawattana, Nitaya, additional, Jutrakul, Yaowaruk, additional, Wuthiekanun, Vanaporn, additional, Limmathurotsakul, Direk, additional, Brett, Paul J., additional, Burtnick, Mary N., additional, Lertmemongkolchai, Ganjana, additional, and Chantratita, Narisara, additional

Published
2018
Full Text
View/download PDF

27. Immune response to recombinant Burkholderia pseudomallei FliC

Author

Koosakulnirand, Sirikamon, primary, Phokrai, Phornpun, additional, Jenjaroen, Kemajittra, additional, Roberts, Rosemary A., additional, Utaisincharoen, Pongsak, additional, Dunachie, Susanna J., additional, Brett, Paul J., additional, Burtnick, Mary N., additional, and Chantratita, Narisara, additional

Published
2018
Full Text
View/download PDF

29. Retrospective Analysis of Fever and Sepsis Patients from Cambodia Reveals Serological Evidence of Melioidosis

Author

Suttisunhakul, Vichaya, primary, Hip, Phireak, additional, Ouch, Pidor, additional, Clark, Danielle V., additional, Ly, Piseth, additional, Lawler, James V., additional, Supaprom, Chonthida, additional, Chantratita, Narisara, additional, Rachmat, Agus, additional, Burtnick, Mary N., additional, Kheng, Sim, additional, Prouty, Michael, additional, Brett, Paul J., additional, Vaughn, Andrew, additional, Schully, Kevin L., additional, and Eltayeb, Ahreej, additional

Published
2018
Full Text
View/download PDF

31. Melioidosis diagnostic workshop, 2013

Author

Hoffmaster, Alex R, AuCoin, David, Baccam, Prasith, Baggett, Henry C, Baird, Rob, Bhengsri, Saithip, Blaney, David D, Brett, Paul J, Brooks, Timothy JG, Brown, Katherine A, Chantratita, Narisara, Cheng, Allen C, Dance, David AB, Decuypere, Saskia, Defenbaugh, Dawn, Gee, Jay E, Houghton, Raymond, Jorakate, Possawat, Lertmemongkolchai, Ganjana, Limmathurotsakul, Direk, Merlin, Toby L, Mukhopadhyay, Chiranjay, Norton, Robert, Peacock, Sharon J, Rolim, Dionne B, Simpson, Andrew J, Steinmetz, Ivo, Stoddard, Robyn A, Stokes, Martha M, Sue, David, Tuanyok, Apichai, Whistler, Toni, Wuthiekanun, Vanaporn, and Walke, Henry T

Abstract

Melioidosis is a severe disease that can be difficult to diagnose because of its diverse clinical manifestations and a lack of adequate diagnostic capabilities for suspected cases. There is broad interest in improving detection and diagnosis of this disease not only in melioidosis-endemic regions but also outside these regions because melioidosis may be underreported and poses a potential bioterrorism challenge for public health authorities. Therefore, a workshop of academic, government, and private sector personnel from around the world was convened to discuss the current state of melioidosis diagnostics, diagnostic needs, and future directions.

Published
2016

33. Deciphering minimal antigenic epitopes associated with Burkholderia pseudomallei and Burkholderia mallei lipopolysaccharide O-antigens

Author

Tamigney Kenfack, Marielle, primary, Mazur, Marcelina, additional, Nualnoi, Teerapat, additional, Shaffer, Teresa L., additional, Ngassimou, Abba, additional, Blériot, Yves, additional, Marrot, Jérôme, additional, Marchetti, Roberta, additional, Sintiprungrat, Kitisak, additional, Chantratita, Narisara, additional, Silipo, Alba, additional, Molinaro, Antonio, additional, AuCoin, David P., additional, Burtnick, Mary N., additional, Brett, Paul J., additional, and Gauthier, Charles, additional

Published
2017
Full Text
View/download PDF

34. Genome-scale analysis of the genes that contribute to Burkholderia pseudomallei biofilm formation identifies a crucial exopolysaccharide biosynthesis gene cluster

Author

Borlee, Grace I., primary, Plumley, Brooke A., additional, Martin, Kevin H., additional, Somprasong, Nawarat, additional, Mangalea, Mihnea R., additional, Islam, M. Nurul, additional, Burtnick, Mary N., additional, Brett, Paul J., additional, Steinmetz, Ivo, additional, AuCoin, David P., additional, Belisle, John T., additional, Crick, Dean C., additional, Schweizer, Herbert P., additional, and Borlee, Bradley R., additional

Published
2017
Full Text
View/download PDF

35. A nonsense mutation in TLR5 is associated with survival and reduced IL-10 and TNF-α levels in human melioidosis

Author

Chaichana, Panjaporn, primary, Chantratita, Narisara, additional, Brod, Florian, additional, Koosakulnirand, Sirikamon, additional, Jenjaroen, Kemajittra, additional, Chumseng, Suchintana, additional, Sumonwiriya, Manutsanun, additional, Burtnick, Mary N., additional, Brett, Paul J., additional, Teparrukkul, Prapit, additional, Limmathurotsakul, Direk, additional, Day, Nicholas P. J., additional, Dunachie, Susanna J., additional, and West, T. Eoin, additional

Published
2017
Full Text
View/download PDF

41. Consensus on the development of vaccines against naturally acquired melioidosis

Author

Limmathurotsakul, Direk, Funnell, Simon GP, Torres, Alfredo G, Morici, Lisa A, Brett, Paul J, Dunachie, Susanna, Atkins, Timothy, Altmann, Daniel M, Bancroft, Gregory, Peacock, Sharon J, and Steering Group on Melioidosis Vaccine Development

Abstract

Several candidates for a vaccine against Burkholderia pseudomallei, the causal bacterium of melioidosis, have been developed, and a rational approach is now needed to select and advance candidates for testing in relevant nonhuman primate models and in human clinical trials. Development of such a vaccine was the topic of a meeting in the United Kingdom in March 2014 attended by international candidate vaccine developers, researchers, and government health officials. The focus of the meeting was advancement of vaccines for prevention of natural infection, rather than for protection from the organism's known potential for use as a biological weapon. A direct comparison of candidate vaccines in well-characterized mouse models was proposed. Knowledge gaps requiring further research were identified. Recommendations were made to accelerate the development of an effective vaccine against melioidosis.

Published
2015

Catalog

Books, media, physical & digital resources
See catalog results