1. Aqueous humor endothelin-1 and total retinal blood flow in patients with non-proliferative diabetic retinopathy
- Author
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Khuu, L-A, Tayyari, F, Sivak, JM, Flanagan, JG, Singer, S, Brent, MH, Huang, D, Tan, O, and Hudson, C
- Subjects
Biomedical and Clinical Sciences ,Ophthalmology and Optometry ,Diabetes ,Eye Disease and Disorders of Vision ,Clinical Research ,Eye ,Metabolic and endocrine ,Aged ,Aqueous Humor ,Biomarkers ,Diabetes Mellitus ,Type 2 ,Diabetic Retinopathy ,Endothelin-1 ,Female ,Humans ,Male ,Regional Blood Flow ,Retinal Vessels ,Tomography ,Optical Coherence ,Clinical Sciences ,Immunology ,Opthalmology and Optometry ,Ophthalmology & Optometry ,Ophthalmology and optometry - Abstract
PurposeThe purpose of this study was to determine the association between aqueous ET-1 levels and total retinal blood flow (TRBF) in patients with non-insulin-dependent type 2 diabetes mellitus (T2DM) and early non-proliferative diabetic retinopathy (NPDR).Patients and methodsA total of 15 age-matched controls and 15 T2DM patients with NPDR were recruited into the study. Aqueous humor (~80-120 μl) was collected before cataract surgery to measure the levels of ET-1 using suspension multiplex array technology. Four weeks post surgery, six images were acquired to assess TRBF using the prototype RTVue Doppler FD-OCT (Optovue, Inc., Fremont, CA, USA) with a double circular scan protocol. At the same visit, forearm blood was collected to determine plasma glycosylated hemoglobin (A1c) levels.ResultsAqueous ET-1 was significantly elevated in the NPDR group compared with the control group (3.5±1.8 vs 2.2±0.8, P=0.02). TRBF was found to be significantly reduced in the NPDR group compared with the control group (34.5±9.1 vs 44.1±4.6 μl/min, P=0.002). TRBF and aqueous ET-1 were not correlated within the NPDR group (r=-0.24, P=0.22). In a multivariate analysis, high A1c was associated with reduced TRBF and aqueous ET-1 levels across control and NPDR groups (P
- Published
- 2017