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A study to evaluate the addition of water-soluble polyphenolics extracted from rosemary and thyme, concentrated and purified by reverse phase chromatography is illustrated. The results indicated that extraction and purification regime not only improved the stability but also increased the initial color intensity and antioxidant capacity of the juices.

Botrytis cinerea (grey mould) is the causal agent of grey rot in strawberries. Worldwide, it causes substantial pre- and postharvest losses (40 - 60%), attacking over 1,400 crops. To combat this phytopathogenic fungus, synthetic preservatives including potassium sorbate (PS) are used, and other barrier technologies have been investigated. Avocado seed acetogenins (ASAs) have been studied for their antibacterial activity but not for their antifungal effect. The effect of ASAs against mycelial growth and conidia of B. cinerea in vitro is unknown. Therefore, the aim of the present work was to investigate the antifungal effect of a crude avocado seed extract enriched with acetogenins (Avosafe®) against mycelial growth and conidial germination of B. cinerea in vitro. Twelve Avosafe® treatments were tested using an agar extract dilution method. The minimum inhibitory concentration (MIC) and the antifungal effect of Avosafe® on mycelial growth and conidial germination were tested after 96 and 5 h of incubation at 18 ± 1°C, respectively. An adjusted kinetic Gompertz model was used to determine the growth phase in which Avosafe® affected mycelial growth and conidial germination. Avosafe® at a concentration of 7,500 mg/L directly affected the stationary phase of the developing fungus with no significant differences (p > 0.05) with typical usage level of PS (1,000 mg/L). The mean fungistatic concentration of mycelial growth (661 mg/L) was associated with a 78% reduction in the percentage of conidial germination, whereas its IC50 at this stage was 75.42 mg/L. Therefore, Avosafe® could be a possible alternative as natural fungistatic agent against B. cinerea in processed food products such as strawberry purée. [ABSTRACT FROM AUTHOR]

In traditional yogurt manufacturing, the yogurt is not agitated during fermentation. However, stirring could be beneficial, particularly for improving heat and mass transport across the fermentation tank. In this contribution, we studied the effect of low-speed agitation during fermentation on process time, acidity profile, and microbial dynamics during yogurt fermentation in 2 laboratory-scale fermenters (3 and 5 L) with different heat-transfer characteristics. Lactobacillus bulgaricus and Streptococcus thermophilus were used as fermenting bacteria. Curves of pH, lactic acid concentration, lactose concentration, and bacterial population profiles during fermentation are presented for static and low-agitation conditions during fermentation. At low-inoculum conditions, agitation reduced the processing time by shortening the lag phase. However, mixing did not modify the duration or the shape of the pH profiles during the exponential phase. In fermentors with poor heat-transfer characteristics, important differences in microbial dynamics were observed between the agitated and nonagitated fermentation experiments; that is, agitation significantly increased the observable specific growth rate and the final microbial count of L. bulgaricus. [ABSTRACT FROM AUTHOR]

Fatty acid (FA), tocopherol, and phytosterol profiles are used in avocado oil purity standards. However, blends with other oils can mimic the profile of pure avocado oil, resulting in similar ranges for these molecules. Therefore, fatty alcohol esters (FAEs) uniquely of Persea spp. were assessed as complementary markers in the chemical profile of pure avocado oil. Utilizing HPLC-TOF-MS and UPLC-MS/MS for identification and HPLC-DAD-ELSD for quantification, major FAEs were analyzed in nine cultivars. Major FAEs included Persin (20-48%), AcO-avocadyne (11-44%), Persenone A (12-30%), and AcO-avocadene (3-24%). Metabolic balance between the main FAs (C18:1n-9, C16:0, C16:1n-9, and C18:2n-6), vaccenic (C18:1n-7), two C17-FAs, and low levels of C18:3n-3 and stearic acid marked the pattern. Relationships between avocado FAs and FAEs were assessed, reinforcing the relevance of both metabolite groups to the fingerprinting of avocado oil and as novel composition biomarkers.

This study investigated the potential health benefits of spearmint, orange peel, and baby sage oleoresins extracted using supercritical CO 2 and subsequently emulsified. The oleoresins were incorporated into dark chocolate, and their impact on physicochemical properties was evaluated. Characterization revealed rich sources of phenolic compounds, carotenoids, and volatile compounds in these oleoresins. In vitro studies demonstrated anti-obesogenic, antioxidant, anti-inflammatory, and neuroprotective properties of the emulsified oleoresins. However, only physicochemical properties were determined for the formulations of dark chocolate with these emulsified oleoresins. Chocolate formulations fortified with these emulsions displayed a softer texture, lower water activity, and solid-like behavior. The findings suggest that these oleoresins could serve as nutraceutical agents for mitigating metabolic syndrome and associated pathologies. Incorporating them into chocolate matrices offers a practical approach to formulating functional foods. Further research is warranted to explore the preventive and therapeutic efficacy in an in vivo model., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Fraud in alcoholic beverages through counterfeiting and adulteration is rising, significantly impacting companies economically. This study aimed to develop a method using near-infrared (NIR) spectroscopy (1596-2396 nm) through the bottle, along with machine learning (ML) modeling for beer authentication, quality traits, and control assessment. For this study, 25 commercial beers from different brands, styles, and three types of fermentation were used. To obtain the ground-truth data, a quantitative descriptive analysis was conducted with 11 trained panelists to evaluate the intensity of 16 sensory descriptors, and volatile aromatic compounds were analyzed using gas chromatography-mass spectroscopy (GC-MS). The ML models were developed using artificial neural networks with NIR absorbance values as inputs to predict (i) type of fermentation (Model 1), (ii) intensity of 16 sensory descriptors (Model 2), and (iii) peak area of volatile aromatic compounds (Model 3). All models resulted in high overall accuracy (Model 1: 99%; Model 2: R = 0.92; Model 3: R = 0.94), and model deployment for new beer samples showed high performance (Model 1: 95%; Model 2: R = 0.83). This method enables brewers and retailers to analyze beers without opening bottles, preventing quality assurance issues, fraud, and provenance concerns. Further model training with new targets could assess additional quality traits like physicochemical parameters and origin. PRACTICAL APPLICATION: Near-infrared spectroscopy coupled with ML modeling is a novel method for assessing beer quality and authentication through the bottle. It serves as a rapid, accurate tool for predicting sensory and aroma profiles without opening the bottle. Additionally, it monitors quality traits during transport and storage., (© 2025 The Author(s). Journal of Food Science published by Wiley Periodicals LLC on behalf of Institute of Food Technologists.)

Consumer acceptability of beers is influenced by product formulation and processing conditions, which impart unique sensory profiles. This study used multivariate techniques to evaluate at-home consumer sensory acceptability of six commercial beers considering their style, fermentation type, and chemical composition. Samples included top-fermented beers (American India Pale Ale and Stout) and bottom-fermented beers (Pilsner, zero-alcohol Pilsner, Vienna Lager, and Munich Dunkel). Beer consumers ( n  = 50) conducted sensory hedonic, check-all-that-apply (CATA) and just-about-right (JAR) tests. Chemometric variables included iso-alpha-acids, hordenine, and volatile aromatic compounds, quantified by chromatographic methods, whereas bitterness units (IBU) were determined spectrophotometrically. Lager beers had higher acceptability than top-fermented beer ( p  < .05) for all attributes. Light-colored beers and medium-height foams had the highest liking scores for visual sensory attributes. Higher concentrations of bitter-tasting molecules, hordenine, and acidity decreased the liking scores of top-fermented (Ale) beers, as a sensory penalty analysis suggested. In contrast, the most favored beers (Pilsners and Munich Dunkel) contained higher fusel alcohol esters linked to fruity aromatic notes. Although a low conversion rate of fatty acids into fruity esters was noted in nonalcoholic Pilsner, its overall liking score was not statistically different from the alcoholic version. However, consumers perceived the nonalcoholic Pilsner as less bitter than its alcoholic counterpart even when IBUs were nonsignificantly different. This study emphasized the significance of understanding beer chemometrics to comprehend consumer acceptability, highlighting the crucial role of bitter molecules. Hence, hordenine, acidity, and volatile contents provided additional and valuable insights into consumer preferences., Competing Interests: The authors declare no conflicts of interest., (© 2024 The Authors. Food Science & Nutrition published by Wiley Periodicals LLC.)

Objectives: The study aimed to compare the trueness and precision of five intraoral scanners (Emerald S, iTero Element 5D, Medit i700, Primescan, and Trios 4) and two indirect digitization techniques for both teeth and soft tissues on fresh mandibular and maxillary cadaver jaws., Methods: The maxilla and mandible of a fully dentate cadaver were scanned by the ATOS industrial scanner to create a master model. Then, the specimens were scanned eight times by each intraoral scanner (IOS). In addition, 8 polyvinylsiloxane (PVS) impressions were made and digitized with a Medit T710 desktop scanner. Stone models were then poured and again scanned with the desktop scanner. All IOS, PVS, and stone models were compared to the master model to calculate the mean absolute surface deviation for mandibular teeth, maxillary teeth, and palate., Results: For mandibular teeth, the PVS trueness was only significantly better than the Medit i700 (p < 0.001) and Primescan (p < 0.05). In maxillary teeth, the PVS trueness was significantly better than all IOSs (p < 0.05-0.001); the stone trueness was significantly better than Emerald S (p < 0.01), Medit i700 (p < 0.001) and Primescan (p < 0.01). In the palate, PVS and stone trueness were significantly lower than the iTero Element 5D (p < 0.01) and Trios 4 (p < p < 0.01). Stone trueness was significantly lower than the Medit i700 (p < 0.05). The precision in the palate was significantly lower for PVS and stone than for Emerald S (p < 0.01, p < 0.05), iTero Element 5D (p < 0.01, p < 0.01), Primescan (p < 0.001, p < 0.001), and Trios 4 (p < 0.001, p < 0.01). Significant differences in trueness between the IOSs were observed only in the mandibular teeth. The Medit i700 performed worse than Emerald S (p < 0.01) and iTero Element 5D (p < 0.01). For mandibular teeth, the Medit i700 was significantly more precise than Primescan (p < 0.01) and the Emerald S (p < 0.05). The Trios 4 was significantly less precise than Emerald S (p < 0.05). The precision of Medit i700 was significantly worse than iTero Element 5D (p < 0.01) for maxillary teeth, as well as the Primescan (p < 0.01) and Trios 4 (p < 0.05) for the palate., Conclusions: In general, indirectly digitized models from PVS impressions had higher trueness than IOS for maxillary teeth; precision between the two methods was similar. IOS was more accurate for palatal tissues. The differences in trueness and precision for mandibular teeth between the various techniques were negligible., Clinical Significance: All investigated IOSs and indirect digitization could be used for complete arch scanning in mandibular and maxillary dentate arches. However, direct optical digitization is preferable for the palate due to the low accuracy of physical impression techniques for soft tissues., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have influenced the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Micronutrient deficiencies are widespread and growing global concerns. Nanoscale nutrients present higher absorption rates and improved nutrient availability and nutrient use efficiency. Co-application of nanofertilizers (NFs) with biological agents or organic compounds increases NF biocompatibility, stability, and efficacy. This study aimed to develop and evaluate zinc and iron bio-nanofertilizers formulated with plant growth-promoting rhizobacteria (PGPR) and microalgae. Nanoparticles (NPs) were synthesized with the co-precipitation method and functionalized with Pseudomonas species and Spirulina platensis preparation. NPs were characterized and evaluated on seed germination, soil microbial growth, and early plant response under seedbed conditions. NPs corresponded to zinc oxide (ZnO; 77 nm) and maghemite (γ-Fe 2 O 3 ; 68 nm). Functionalized nanoparticles showed larger sizes, around 145-233 nm. The seedling vigor index of tomato and maize was significantly increased (32.9-46.1%) by bacteria-functionalized ZnO- and γ-Fe 2 O 3 -NPs at 75 ppm. NFs at 250 and 75 ppm significantly increased bacterial growth. NFs also improved early plant growth by increasing plant height (14-44%), leaf diameter (22-47%), and fresh weight (46-119%) in broccoli and radish, which were mainly influenced by bacteria capped ZnO- and γ-Fe 2 O 3 -NPs at 250 ppm. Beneficial effects on plant growth can be attributed to the synergistic interaction of the biological components and the zinc and iron NPs in the bio-nanofertilizers.

Objectives: The objectives of this study were to quantify the number and type of prosthetic complications associated with 3D-printed implant-supported fixed prostheses (3DISFP) and to evaluate patient satisfaction and oral health-related quality of life over a four-month period., Methods:  Fifteen edentulous patients who underwent implant therapy were included in the study. Each patient received a 3D-printed prosthesis using OnX dental resin. Prosthetic complications were documented, and data from the 14-item Oral Health Impact Profile (OHIP) questionnaire were collected at two time points: at enrollment and during a four-month recall., Results: During the four-month evaluation period, a total of nine complications were recorded, with three classified as catastrophic failures. Statistical analysis revealed statistically significant differences in OHIP scores between the preoperative and postoperative assessments (p<0.001)., Conclusion: Within the limitations of this study, it can be concluded that utilizing 3D-printed prostheses with OnX resin represents a viable alternative for long-term implant-supported temporaries. The patients experienced a significant improvement in their oral health-related quality of life. These results suggest that 3D printing technology, combined with the use of OnX resin, holds promise in providing satisfactory clinical outcomes and enhanced patient satisfaction. However, it is important to acknowledge the limitations of this study, and further research is warranted to validate these findings and explore the long-term performance and durability of 3D-printed implant-supported fixed prostheses. This study contributes to the growing body of evidence supporting the effectiveness of 3D printing technology in implant dentistry. The results highlight the potential of 3DISFP with OnX resin to improve oral health-related quality of life in edentulous patients. Continued advancements in 3D printing materials and techniques will likely expand the utilization of these prostheses, ultimately benefiting patients in need of implant-supported restorations., Competing Interests: Dr. Brenes is a Key Opinion Leader for Sprintray and has provided webinars and lectures in the past for the company., (Copyright © 2023, Brenes et al.)

Gut metagenome in pediatric subjects with metabolic syndrome (MetS) and type-2 diabetes mellitus (T2DM) has been poorly studied, despite an alarming worldwide increase in the prevalence and incidence of obesity and MetS within this population. The objective of this study was to characterize the gut microbiome taxonomic composition of Mexican pediatric subjects with MetS and T2DM using shotgun metagenomics and analyze the potential relationship with metabolic changes and proinflammatory effects. Paired-end reads of fecal DNA samples were obtained through the Illumina HiSeq X Platform. Statistical analyses and correlational studies were conducted using gut microbiome data and metadata from all individuals. Gut microbial dysbiosis was observed in MetS and T2DM children compared to healthy subjects, which was characterized by an increase in facultative anaerobes (i.e., enteric and lactic acid bacteria) and a decrease in strict anaerobes (i.e., Erysipelatoclostridium , Shaalia , and Actinomyces genera). This may cause a loss of gut hypoxic environment, increased gut microbial nitrogen metabolism, and higher production of pathogen-associated molecular patterns. These metabolic changes may trigger the activation of proinflammatory activity and impair the host's intermediate metabolism, leading to a possible progression of the characteristic risk factors of MetS and T2DM, such as insulin resistance, dyslipidemia, and an increased abdominal circumference. Furthermore, specific viruses ( Jiaodavirus genus and Inoviridae family) showed positive correlations with proinflammatory cytokines involved in these metabolic diseases. This study provides novel evidence for the characterization of MetS and T2DM pediatric subjects in which the whole gut microbial composition has been characterized. Additionally, it describes specific gut microorganisms with functional changes that may influence the onset of relevant health risk factors., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (© 2023 Carrizales-Sánchez, Tamez-Rivera, García-Gamboa, García-Cayuela, Rodríguez-Gutiérrez, Elizondo-Montemayor, García-Rivas, Pacheco, Hernández-Brenes, and Senés-Guerrero.)

This study compared the accuracy of implant scan bodies printed using stereolithography (SLA) and digital light processing (DLP) technologies to the control (manufacturer's scan body) Scan bodies were printed using SLA (n = 10) and DLP (n = 10) methods. Ten manufacturer's scan bodies were used as control. The scan body was placed onto a simulated 3D printed cast with a single implant placed. An implant fixture mount was used as standard. The implant positions were scanned using a laboratory scanner with the fixture mounts, manufacturer's scan bodies, and the printed scan bodies. The scans of each scan body was then superimposed onto the referenced fixture mount. The 3D angulation and linear deviations were measured. The angulation and linear deviations were 1.24±0.22° and 0.20±0.05 mm; 2.63±0.82° and 0.34±0.11 mm; 1.79±0.19° and 0.32±0.03 mm; for the control, SLA, and DLP, respectively. There were statistical differences (ANOVA) among the three groups in the angular (p<0.01) or linear deviations (p<0.01). Box plotting, 95% confidence interval and F-test suggested the higher variations of precision in the SLA group compared to DLP and control groups. Scan bodies printed in-office have lower accuracy compared to the manufacturer's scan bodies. The current technology for 3D printing of implant scan bodies needs trueness and precision improvements., Competing Interests: Author Sompop Bencharit is a paid consultant and speaker for Formlabs and ZimVie, outside the submitted work. Author Christian Brenes is also a paid consultant and speaker for Sprinkray, outside the submitted work. The manufacturers did not provide any support to this project and did not play any role in the experimental design, result interpretation, manuscript preparation, or the submission of this work. There are no patents, products in development or marketed products associated with this research to declare. This does not alter our adherence to PLOS ONE policies on sharing data and materials., (Copyright: © 2023 Hopfensperger et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

The obesity pandemic has been strongly associated with the Western diet, characterized by the consumption of ultra-processed foods. The Western lifestyle causes gut dysbiosis leading to impaired fatty acid metabolism. Therefore, this study aimed to evaluate shifts in gut microbiota and correlate these with serum fatty acid profiles in male Wistar rats fed a cafeteria diet. Ten male rats were fed with standard diet (CTL, n = 5) and cafeteria diet (CAF, n = 5) for fifteen weeks. Body weight and food intake were recorded once and three times per week, respectively. At the end of the study, fresh fecal samples were collected, tissues were removed, and serum samples were obtained for further analyses. Gut microbiota was analyzed by sequencing the V3-V4 region of 16S rRNA gene. Serum fatty acid profiles were fractioned and quantified via gas chromatography. The CAF diet induced an obese phenotype accompanied by impaired serum fatty acids, finding significantly higher proportions of total saturated fatty acids (SFAs) and C20:3 n -6, and lower C18:1 n -7 and C18:3 n -3 in the phospholipid (PL) fraction. Furthermore, circulating C10:0, total n -3 and n -7 decreased and total monounsaturated fatty acids (MUFAs), including oleic acid C18:1 n -9, increased in the cholesterol ester (CE) fraction. The obesity metabotype may be mediated by gut dysbiosis caused by a cafeteria diet rich in C16:0, C18:0, C18:1 n -9 and C18:2 n -6 fatty acids resulting in a 34:1 omega-6/omega-3 ratio. Therefore, circulating C10:0 was associated with several genera bacteria such as Prevotella (positive) and Anaerotruncus (negative). Two classes of Firmicutes , Bacilli and Erysipelotrichi , were positively correlated with PL- C20:3 n -6 and CE- 18:1 n -9, respectively. TM7 and Bacteroidetes were inversely correlated with PL-SFAs and CE- 18:2 n -6, respectively.

Background: Cognition and brain function is critical through childhood and should be improved with balanced diets. Incorporating bioactive ingredients such as omega-3 polyunsaturated fatty acids (ω3 PUFAs) and probiotics into food formulations could be used as an approach to improve cognitive function. This study evaluated the effects on cognitive capacity of complementing rodent diets with chocolate, by itself and in combination with ω3 PUFAs from fish oil and probiotics., Methods: Spatial learning and memory in the rats were determined by the Barnes maze test in short- and long-term memory. Samples from the cecum were obtained to assess microbial counts ( Lactobacillus, Bifidobacterium, Enterobacteriaceae , and total bacteria), and brains were recovered to analyze the neural morphology of the tissues. Also, glucose, brain weights, and epididymal tissue were analyzed., Results: The combination of chocolate with fish oil and probiotics improved the memory of rats compared to the result of each bioactive compound when evaluated separately. Treatments did not affect sugar level, epididymal adipose tissue, or brain weight. On the other hand, consuming probiotics alone or in combination with chocolate decreased Enterobacteria counts, while Lactobacillus and Bifidobacteria counts were not affected. Neural morphological analysis showed that combining chocolate with probiotics and ω3 PUFAs increased the number of neurons in the hippocampal CA1 and CA3 regions., Conclusion: Chocolate added with probiotics and ω3 PUFAs improved spatial memory and learning in the studied model., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Faccinetto-Beltrán, Aguirre-López, Bañuelos-Pineda, Reza-Zaldívar, Santacruz, Hernández-Brenes, Pérez-Carrillo and Jacobo-Velázquez.)

The social isolation settings derived from the COVID-19 pandemic affected the standard sensory evaluation techniques used in the food and beverage industry. This situation forced companies and researchers to assess other options to continue conducting these tests in remote contactless locations. This study aimed to evaluate two sets of samples (i) six images from Geneva affective picture database (GAPED) and (ii) six videos of beer pouring using traditional self-reported sensory data and emotional response from consumers biometrics. Specifically, four research questions (RQ) arouse from this study: RQ1: are there significant differences between GAPED images and beers in unconscious and self-reported responses from consumers?, RQ2: are there any correlations between subconscious and self-reported responses from consumers when assessing beer?, RQ3: can consumers differentiate positive, neutral and negative images based on subconscious and self-reported responses?, RQ4: are there any relationships between subconscious and self-reported responses when assessing GAPED images and beers, and how are samples associated with variables? A total of 113 Mexican beer consumers participated in the virtual sensory session using an online videoconference software to record videos of participants during the session. Results showed there were significant differences (p < 0.05) between samples, especially for self-reported responses (RQ1), and several correlations between variables, such as positive correlations between the perceived quality of beers and happy emoji (r = 0.84), and negative correlation with confused emoji (r = -0.97; RQ2). Besides, using the proposed methods, consumers were able to correctly differentiate through elicited emotions the positive, neutral and negative GAPED images (RQ3). Regarding RQ4, several relationships were found between variables in both GAPED images and beers; however, it was found that different emotions were elicited depending of the stimuli used. The proposed method showed to be a reliable and practical option to conduct visual and potentially tasting sensory tests in isolation and recruit participants from different countries without travelling to collect their responses., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Lipids in avocados have been widely studied due to their nutritional value and several reported bioactivities. Aliphatic acetogenins are a relevant component of the avocado lipidome and have been tested for several potential food and pharma industries applications. This work followed the evolution of avocado fatty acids (FAs) and aliphatic acetogenins during seed germination and leaf growth. Oil extracts of embryonic axes, cotyledons, and leaves from seedlings and trees were divided to analyze free acetylated acetogenins (AcO-acetogenins), and free FAs. Embryonic axes from germinating seeds contained the highest amount of AcO-acetogenins and FAs; this tissue also accumulated the most diverse FA profile with up to 22 detected moieties. Leaves presented the highest variations in AcO-acetogenin profiles during development, although leaves from seedlings accumulated the simplest FA profile with only 10 different FAs. Remarkably, AcO-acetogenins represented half of the carbons allocated to lipids in grown leaves, while embryonic axes and cotyledons always contained more carbons within FAs during germination. Thus, we hypothesized the use of the AcO-acetogenin acyl chain for energy production toward β-oxidation. Also, α-linolenic and docosahexaenoic acids (DHAs) were proposed as close AcO-acetogenin intermediaries based on a correlation network generated using all these data. Another part of the oil extract was fractionated into different lipid classes before transesterification to profile FAs and acetogenins bound to lipids. Acetogenin backbones were identified for the first time in triglycerides from cotyledons and mainly in polar lipids (which include phospholipids) in all developing avocado tissues analyzed. Seed tissues accumulated preferentially polar lipids during germination, while triglycerides were consumed in cotyledons. Seedling leaves contained minute amounts of triglycerides, and polar lipids increased as they developed. Results from this work suggest acetogenins might be part of the energy and signaling metabolisms, and possibly of membrane structures, underlining the yet to establish role(s) of these unusual lipids in the avocado plant physiology., Competing Interests: CH-B, DR-S, and RD are inventors for several acetogenins in the food, pharmaceutical, and personal care industries, which are under patent applications PCT/IB2011/053535 and PCT/IB2015/002021 and US2018013671A1 and MX 3355202 B. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Colin-Oviedo, Garza-Aguilar, Marín-Obispo, Rodríguez-Sánchez, Trevino, Hernández-Brenes and Díaz de la Garza.)

Chocolate is an adequate matrix to deliver bioactive ingredients. However, it contains high sugar levels, one of the leading causes of chronic degenerative diseases. This work aimed to evaluate the effects of milk chocolate reformulation with alternative sugar sweeteners (Sw; isomalt + stevia), probiotics (Prob), and ω-3 polyunsaturated fatty acids (PUFAs) on its physicochemical properties and consumers' acceptability. Lactobacillus plantarum 299v (L. p299v) and Lactobacillus acidophilus La3 (DSMZ 17742) were added as Prob strains, and fish oil (FO) was added as the source of ω-3 PUFAs. Prob addition resulted in chocolates with >2 × 10 7 colony forming unit (CFU) per serving size (12 g). Except for Prob, a w values of all treatments were <0.46. Sw and Sw + Prob presented the nearest values to the control in hardness, whereas Sw without FO increased fracturability. FO, Sw + FO, and Sw + Prob + FO contained 107.4 ± 12.84, 142.9 ± 17.9, and 133.78 ± 8.76 mg of ω-3 PUFAs per chocolate, respectively. Prob + FO increased the resistance of chocolate to shear stress, while Sw + FO showed a similar flow behavior to the control. The consumers' acceptability of Sw + Prob chocolate was adequate, while Sw + Prob + FO had higher acceptability than Prob + FO. Health benefits of reformulated milk chocolates requires further assessment by in vitro, in vivo and clinical studies.

In this study, a milk chocolate formulation was developed to serve as vehicle of Omega-3 (ω3) polyunsaturated fatty acids (PUFAs) and probiotics ( L. plantarum 299v and L. rhamnosus GG). Fish oil (FO) was incorporated in chocolate as a source of ω3 PUFAs. Probiotics (Prob) and FO were added during tempering, obtaining chocolates with 76.0 ± 5.2 mg (FO1) or 195.8 ± 6.5 mg (FO2) of ω3 PUFAs, and >1 × 10 6 CFU of Prob per chocolate portion (12 g). The physicochemical properties (rheological analysis, texture, surface instrumental color, aw, and fatty acid profile), and sensory acceptability of the formulations were determined. Prob and FO generated a decrease in L * and white index (WI) values. Except for Prob + FO2, all treatments showed a decrease in aw. Rheological parameters of FO1 and Prob + FO1 presented the most similar behavior as compared with the control. Prob or FO1 addition did not affect the overall consumer's acceptability of chocolate; and when both nutraceuticals were combined (Prob + FO1) the product showed adequate overall acceptability. FO2 formulations were not considered adequate to maintain physicochemical properties and sensory acceptability of chocolate. Results indicated that milk chocolate is a suitable vehicle for delivering ω3 PUFAs and Prob, which are essential to enhance cognitive development in children.

Trends in new food products focus on low-carbohydrate ingredients rich in healthy fats, proteins, and micronutrients; thus, avocado has gained worldwide attention. This study aimed to use predictive modeling to identify the potential sensory drivers of liking for avocado pulp by evaluating acceptability scores and sensory descriptive profiles of two commercial and five non-commercial cultivars. Macronutrient composition, instrumental texture, and color were also characterized. Trained panelists performed a descriptive profile of nineteen sensory attributes. Affective data from frequent avocado adult consumers ( n = 116) were collected for predictive modeling of an external preference map ( R 2 = 0.98), which provided insight into sensory descriptors that drove preference for particular avocado pulps. The descriptive map explained 67.6% of the variance in sensory profiles. Most accepted pulps were from Hass and Colin V-33; the latter had sweet and green flavor notes. Descriptive flavor attributes related to liking were global impact, oily, and creamy. Sensory drivers of texture liking included creamy/oily, lipid residue, firmness, and cohesiveness. Instrumental stickiness was disliked and inversely correlated to dry-matter and lipids ( r = -0.87 and -0.79, respectively). Color differences (∆E ab *) also contributed to dislike. Sensory-guided selection of avocado fruits and ingredients can develop products with high acceptability in breeding and industrialization strategies.

Childhood obesity and T2DM have shown a recent alarming increase due to important changes in global lifestyle and dietary habits, highlighting the need for urgent and novel solutions to improve global public health. Gut microbiota has been shown to be relevant in human health and its dysbiosis has been associated with MetS, a health condition linked to the onset of relevant diseases including T2DM. Even though there have been recent improvements in the understanding of gut microbiota-host interactions, pediatric gut microbiota has been poorly studied compared to adults. This review provides an overview of MetS and its relevance in school-age children, discusses gut microbiota and its possible association with this metabolic condition including relevant emerging gut microbiome-based interventions for its prevention and treatment, and outlines future challenges and perspectives in preventing microbiota dysbiosis from the early stages of life.

Additive manufacturing processes are increasingly being used in dentistry. The underlying process is the polymerization or fusion of material layer by layer to create layer lines on the final printed surface. How print orientation affects these layer lines is unclear. The primary objective of this research was to measure and compare the surface waviness and surface roughness of maxillary models fabricated using a variety of 3D printers and resin types, and to evaluate the effect of different print angulations. The same STL file was used to manufacture 48 models using a variety of resins and printers at 0 degrees, 30 degrees (with base supports), and 70 degrees ("vertical" without supports) to the build plate. Six replications with each angulation were printed. All samples were optically scanned with a laser profilometer and compared. The results indicated that print angulation can significantly affect the surface roughness of 3D-printed objects, but the results seemed to be specific to the resin/printer products and angles. The authors concluded that surface waviness values should be considered whenever surface smoothness is evaluated.

Hypertension, central obesity, hyperglycemia, and dyslipidemia are key risk factors for cardiovascular disease. However, the specific factors contributing to the development of unfavorable cardiometabolic characteristics in children with obesity are unknown. In this study, we investigated the cross-sectional relationships between cytokines, irisin, and fatty acid (FA) composition in plasma in school-age children with metabolically healthy and unhealthy obesity (MHO and MUO, respectively) of the same age and body mass index and waist circumference percentiles. We compared the data with that of children with normal weight (NW). We found that inflammatory cytokines and low irisin plasma concentrations are associated with obesity but not with cardiometabolic risk (CMR). Lipid profiles showed that children with MUO have a distinctive FA profile compared with children with MHO and NW, whereas children with MHO shared 88% of the FA profile with the NW group. Among all FAs, concentration of myristic acid (14 : 0), arachidic acid (20 : 0), and n-3 polyunsaturated FAs (PUFAs) was higher in children with MHO, whereas n-6 PUFAs such as arachidonic acid (20 : 4n6) had a significant contribution in defining MUO. These data suggest that the plasma FA profile is not only a central link to obesity but also may act as an indicator of CMR presence., Competing Interests: The authors declare no conflict of interest., (Copyright © 2020 Elena Cristina Castillo et al.)

Some chemical compounds, especially alcohol, sugars, and alkaloids such as hordenine, have been reported as elicitors of different emotional responses. This preliminary study was based on six commercial beers selected according to their fermentation type, with two beers of each type (spontaneous, bottom, and top). Chemometry and sensory analysis were performed for all samples to determine relationships and patterns between chemical composition and emotional responses from consumers. The results showed that sweeter samples were associated with higher perceived liking by consumers and positive emotions, which corresponded to spontaneous fermentation beers. There was high correlation ( R = 0.91; R 2 = 0.83) between hordenine and alcohol content. Beers presenting higher concentrations of both, and higher bitterness, were related to negative emotions. Further studies should be conducted, giving more time for emotional response analysis between beer samples, and comparing alcoholic and non-alcoholic beers with similar styles, to separate the effects of alcohol and hordenine. This preliminary study was a first attempt to associate beer compounds with the emotional responses of consumers using non-invasive biometrics.

Purpose: This study was aimed to determine the impact of hydroxytyrosol (HT), a minor compound found in olive oil, on breast cancer stem cells (BCSCs) and the migration capacity of triple-negative breast cancer (TNBC) cell lines through the alteration of epithelial-to-mesenchymal transition (EMT) and embryonic signaling pathways., Methods: BCSCs self-renewal was determined by the mammosphere-forming efficiency in SUM159PT, BT549, MDA-MB-231 and Hs578T TNBC cell lines. Flow cytometric analysis of CD44 + /CD24 -/low and aldehyde dehydrogenase positive (ALDH + ) subpopulations, migration by the "wound healing assay", invasion and Western blot of EMT markers and TGFβ signaling were investigated in SUM159PT, BT549 and MDA-MB-231 cell lines. Wnt/β-catenin signaling was assessed by Western blot in BT549 cells expressing WNT1 and MDA-MB-231 cells. Changes in TGFβ activity was determined by SMAD Binding Element (SBE) reporter assay., Results: HT reduced BCSCs self-renewal, ALDH + (aldehyde dehydrogenase) and CD44 + /CD24 -/low subpopulations, tumor cell migration and invasion. Consistently, HT suppressed Wnt/β-catenin signaling by decreasing p-LRP6, LRP6, β-catenin and cyclin D1 protein expression and the EMT markers SLUG, ZEB1, SNAIL and VIMENTIN. Finally, HT inhibited p-SMAD2/3 and SMAD2/3 in SUM159PT, BT549 and MDA-MB-231 cells, what was correlated with a less TGFβ activity., Conclusion: In conclusion, we report for the first time the inhibitory role of HT on BCSCs and tumor cell migration by targeting EMT, Wnt/β-catenin and TGFβ signaling pathways. Our findings highlight the importance of the chemopreventive compound HT as a novel candidate to be investigated as an alternative targeted therapy for TNBC.

Purpose: To evaluate the marginal fit of CAD/CAM all ceramic crowns made from lithium disilicate and zirconia using two different fabrication protocols (model and model-less)., Materials and Methods: Forty anterior all ceramic restorations (20 lithium disilicate, 20 zirconia) were fabricated from digital impressions using a CEREC Bluecam scanner. Two different digital workflows were used: a fully digital model-less approach and a printed model digital approach. The crowns were cemented on the respective prepared typodont teeth and marginal gap was evaluated using Micro-CT. Each specimen was analyzed in sagittal and trans-axial orientations, allowing evaluation of the marginal fit (vertical and horizontal) on each surface. Logarithmic transformation was used with a significance of 0.05. After that a reliability analysis was performed by re-measuring four randomized selected images for each specimen and performing intraclass correlations to determine any systematic bias in the measurements., Results: Vertical measurements in the lingual, distal and mesial views had an estimated marginal gap ranging from 101.9 to 133.9 µm for lithium disilicate crowns and 126.4 to 165.4 µm for zirconia. No significant differences were found between model and model-less techniques., Conclusions: Both workflows are valid protocols for the fabrication of monolithic ceramic restorations. The use of a printed model did not improve the marginal fit of lithium disilicate or zirconia crowns. Both materials are also clinically acceptable, no matter which workflow was used to obtain the restoration., (© 2019 by the American College of Prosthodontists.)

Acetogenins are bioactive fatty acid derivatives found in avocado tissues. Their efficacy as antimicrobials has been documented and initiated interest to use them as replacements of synthetic food additives. The present work focused on evaluation of multiple analytical methodologies for detection and quantification of organic solids present in a food-grade acetogenin-enriched extract (Avosafe ® ), and on its safety evaluations using bacterial reverse mutation (AMES) tests and acute oral toxicity to rat assays. Results confirmed chemical structures of two acetogenins as present in Avosafe ® (AcO-avocadyne-( 0 ) and AcO-avocadiene B-( 3 )), and together with seven other previously known compounds, quantified 94.74 ± 5.77% w / w of its solids as acetogenins. Safety evaluations indicated that Avosafe ® was non-mutagenic and had an acute median lethal oral dose (LD 50 ) to rats higher than the maximum concentration tested (>2000 mg·kg -1 ), with no signs of macroscopic abnormalities in organs. Mean body weight and hematological and biochemical parameters were normal after 14 days of a single oral dose of 2000 mg·kg -1 . The results advance scientific information on the safety of avocado seed acetogenins and also generate new knowledge on profiles and concentrations of individual acetogenins found in avocado tissues (seed, pulp, and leaves) and in Avosafe ® .

Porous tantalum trabecular metal (PTTM) has long been used in orthopedics to enhance neovascularization, wound healing, and osteogenesis; recently, it has been incorporated into titanium alloy dental implants. However, little is known about the biological responses to PTTM in the human oral cavity. We have hypothesized that, compared with conventional titanium alloy, PTTM has a greater expression of genes specific to neovascularization, wound healing, and osteogenesis during the initial healing period. Twelve subjects requiring at least 4 implants in the mandible were enrolled. Four 3 × 5mm devices, including 2 titanium alloy tapered screws and 2 PTTM cylinders, were placed in the edentulous mandibular areas using a split-mouth design. One device in each group was trephined for analysis at 2 and 4 weeks after placement. RNA microarray analysis and ingenuity pathway analysis were used to analyze osteogenesis gene expression and relevant signaling pathways. Compared to titanium alloy, PTTM samples exhibited significantly higher expressions of genes specific to cell neovascularization, wound healing, and osteogenesis. Several genes-including bone morphogenic proteins, collagens, and growth factors-were upregulated in the PTTM group compared to the titanium alloy control. PTTM materials may enhance the initial healing of dental implants by modifying gene expression profiles.