Search

Objectives The goal of this quality initiative was to develop consensus standards for glycemic management of patients with diabetes who undergo surgical procedures in Canada. Methods A modified Delphi method was used to gather broad stakeholder input and arrive at a consensus for perioperative/periprocedure diabetes management. Results Glycemic management standards were developed for the following categories: Organization of Care; Preoperative Assessment; Immediate Preoperative and Intraoperative; Postanesthesia Care Unit or Recovery Room; Postoperative Period; and Transition to Outpatient Care. Conclusions It is anticipated these standards will serve as a basis to develop clinical tools to support the recommendations.

Aims and objectives. The author will draw on relevant research and her personal experience as someone who lives with a chronic illness to identify the challenges that are inherent in research interviews regarding sexuality in chronic illness. Background. Although sexuality in chronic illness has become a significant body of research in the field of chronic illness, particularly in the field of cancer, there are few guidelines available to assist researchers in interviewing people about such an intimate and sensitive topic. Conclusions. The PLISSIT model used in clinical counselling could be adapted to be used by researchers in interviews about sexuality. With this model a researcher can cover in-depth interview on this individual’s sexuality and sexual health. Also, with the use of the PLISSIT model as a research tool, many of the past myths concerning sexuality and sexual health can be exposed and changed. Relevance to clinical practice. The adaptation of the PLISSIT counselling model to an interviewing model can contribute to researchers feeling more confident with participants when interviewing them concerning their sexuality and sexual health. It may illicit more appropriate responses from individuals concerning their sexuality and sexual health.

SUMO is a ubiquitin-like protein that is post-translationally attached to one or more lysine residues on target proteins. Despite having only 18% sequence identity with ubiquitin, SUMO contains the conserved betabetaalphabetabetaalphabeta fold present in ubiquitin. However, SUMO differs from ubiquitin in having an extended N-terminus. In S. pombe the N-terminus of SUMO/Pmt3 is significantly longer than those of SUMO in S. cerevisiae, human and Drosophila. Here we investigate the role of this N-terminal region. We have used two dimensional gel electrophoresis to demonstrate that S. pombe SUMO/Pmt3 is phosphorylated, and that this occurs on serine residues at the extreme N-terminus of the protein. Mutation of these residues (in pmt3-1) results in a dramatic reduction in both the levels of high Mr SUMO-containing species and of total SUMO/Pmt3, indicating that phosphorylation of SUMO/Pmt3 is required for its stability. Despite the significant reduction in high Mr SUMO-containing species, pmt3-1 cells do not display an aberrant cell morphology or sensitivity to genotoxins or stress. Additionally, we demonstrate that two lysine residues in the N-terminus of S. pombe SUMO/Pmt3 (K14 and K30) can act as acceptor sites for SUMO chain formation in vitro. Inability to form SUMO chains results in aberrant cell and nuclear morphologies, including stretched and fragmented chromatin. SUMO chain mutants are sensitive to the DNA synthesis inhibitor, hydroxyurea (HU), but not to other genotoxins, such as UV, MMS or CPT. This implies a role for SUMO chains in the response to replication arrest in S. pombe., published_or_final_version