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1. Independent human mesenchymal stromal cell–derived extracellular vesicle preparations differentially attenuate symptoms in an advanced murine graft-versus-host disease model

Author

Madel, Rabea J., Börger, Verena, Dittrich, Robin, Bremer, Michel, Tertel, Tobias, Phuong, Nhi Ngo Thi, Baba, Hideo A., Kordelas, Lambros, Staubach, Simon, Stein, Frank, Haberkant, Per, Hackl, Matthias, Grillari, Regina, Grillari, Johannes, Buer, Jan, Horn, Peter A., Westendorf, Astrid M., Brandau, Sven, Kirschning, Carsten J., and Giebel, Bernd

Published
2023
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3. A compendium of single extracellular vesicle flow cytometry

Author

Welsh, Joshua A, Arkesteijn, Ger J A, Bremer, Michel, Cimorelli, Michael, Dignat-George, Françoise, Giebel, Bernd, Görgens, André, Hendrix, An, Kuiper, Martine, Lacroix, Romaric, Lannigan, Joanne, van Leeuwen, Ton G, Lozano-Andrés, Estefanía, Rao, Shoaib, Robert, Stéphane, de Rond, Leonie, Tang, Vera A, Tertel, Tobias, Yan, Xiaomei, Wauben, Marca H M, Nolan, John P, Jones, Jennifer C, Nieuwland, Rienk, van der Pol, Edwin, Welsh, Joshua A, Arkesteijn, Ger J A, Bremer, Michel, Cimorelli, Michael, Dignat-George, Françoise, Giebel, Bernd, Görgens, André, Hendrix, An, Kuiper, Martine, Lacroix, Romaric, Lannigan, Joanne, van Leeuwen, Ton G, Lozano-Andrés, Estefanía, Rao, Shoaib, Robert, Stéphane, de Rond, Leonie, Tang, Vera A, Tertel, Tobias, Yan, Xiaomei, Wauben, Marca H M, Nolan, John P, Jones, Jennifer C, Nieuwland, Rienk, and van der Pol, Edwin

Abstract

Flow cytometry (FCM) offers a multiparametric technology capable of characterizing single extracellular vesicles (EVs). However, most flow cytometers are designed to detect cells, which are larger than EVs. Whereas cells exceed the background noise, signals originating from EVs partly overlap with the background noise, thereby making EVs more difficult to detect than cells. This technical mismatch together with complexity of EV-containing fluids causes limitations and challenges with conducting, interpreting and reproducing EV FCM experiments. To address and overcome these challenges, researchers from the International Society for Extracellular Vesicles (ISEV), International Society for Advancement of Cytometry (ISAC), and the International Society on Thrombosis and Haemostasis (ISTH) joined forces and initiated the EV FCM working group. To improve the interpretation, reporting, and reproducibility of future EV FCM data, the EV FCM working group published an ISEV position manuscript outlining a framework of minimum information that should be reported about an FCM experiment on single EVs (MIFlowCyt-EV). However, the framework contains limited background information. Therefore, the goal of this compendium is to provide the background information necessary to design and conduct reproducible EV FCM experiments. This compendium contains background information on EVs, the interaction between light and EVs, FCM hardware, experimental design and preanalytical procedures, sample preparation, assay controls, instrument data acquisition and calibration, EV characterization, and data reporting. Although this compendium focuses on EVs, many concepts and explanations could also be applied to FCM detection of other particles within the EV size range, such as bacteria, lipoprotein particles, milk fat globules, and viruses.

Published
2023

4. A compendium of single extracellular vesicle flow cytometry

Author

IOV Facs, Celbiologie, Welsh, Joshua A, Arkesteijn, Ger J A, Bremer, Michel, Cimorelli, Michael, Dignat-George, Françoise, Giebel, Bernd, Görgens, André, Hendrix, An, Kuiper, Martine, Lacroix, Romaric, Lannigan, Joanne, van Leeuwen, Ton G, Lozano-Andrés, Estefanía, Rao, Shoaib, Robert, Stéphane, de Rond, Leonie, Tang, Vera A, Tertel, Tobias, Yan, Xiaomei, Wauben, Marca H M, Nolan, John P, Jones, Jennifer C, Nieuwland, Rienk, van der Pol, Edwin, IOV Facs, Celbiologie, Welsh, Joshua A, Arkesteijn, Ger J A, Bremer, Michel, Cimorelli, Michael, Dignat-George, Françoise, Giebel, Bernd, Görgens, André, Hendrix, An, Kuiper, Martine, Lacroix, Romaric, Lannigan, Joanne, van Leeuwen, Ton G, Lozano-Andrés, Estefanía, Rao, Shoaib, Robert, Stéphane, de Rond, Leonie, Tang, Vera A, Tertel, Tobias, Yan, Xiaomei, Wauben, Marca H M, Nolan, John P, Jones, Jennifer C, Nieuwland, Rienk, and van der Pol, Edwin

Published
2023

5. A compendium of single extracellular vesicle flow cytometry

Author

Welsh, Joshua A., primary, Arkesteijn, Ger J. A., additional, Bremer, Michel, additional, Cimorelli, Michael, additional, Dignat‐George, Françoise, additional, Giebel, Bernd, additional, Görgens, André, additional, Hendrix, An, additional, Kuiper, Martine, additional, Lacroix, Romaric, additional, Lannigan, Joanne, additional, van Leeuwen, Ton G., additional, Lozano‐Andrés, Estefanía, additional, Rao, Shoaib, additional, Robert, Stéphane, additional, de Rond, Leonie, additional, Tang, Vera A., additional, Tertel, Tobias, additional, Yan, Xiaomei, additional, Wauben, Marca H. M., additional, Nolan, John P., additional, Jones, Jennifer C., additional, Nieuwland, Rienk, additional, and van der Pol, Edwin, additional

Published
2023
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6. A compendium of single extracellular vesicle flow cytometry

Author

Welsh, Joshua A., Arkesteijn, Ger J. A., Bremer, Michel, Cimorelli, Michael, Dignat-George, Françoise, Giebel, Bernd, Görgens, André, Hendrix, An, Kuiper, Martine, Lacroix, Romaric, Lannigan, Joanne, van Leeuwen, Ton G., Lozano-Andrés, Estefanía, Rao, Shoaib, Robert, Stéphane, de Rond, Leonie, Tang, Vera A., Tertel, Tobias, Yan, Xiaomei, Wauben, Marca H. M., Nolan, John P., Jones, Jennifer C., Nieuwland, Rienk, and van der Pol, Edwin

Subjects
Medizin
Abstract

Flow cytometry (FCM) offers a multiparametric technology capable of characterizing single extracellular vesicles (EVs). However, most flow cytometers are designed to detect cells, which are larger than EVs. Whereas cells exceed the background noise, signals originating from EVs partly overlap with the background noise, thereby making EVs more difficult to detect than cells. This technical mismatch together with complexity of EV-containing fluids causes limitations and challenges with conducting, interpreting and reproducing EV FCM experiments. To address and overcome these challenges, researchers from the International Society for Extracellular Vesicles (ISEV), International Society for Advancement of Cytometry (ISAC), and the International Society on Thrombosis and Haemostasis (ISTH) joined forces and initiated the EV FCM working group. To improve the interpretation, reporting, and reproducibility of future EV FCM data, the EV FCM working group published an ISEV position manuscript outlining a framework of minimum information that should be reported about an FCM experiment on single EVs (MIFlowCyt-EV). However, the framework contains limited background information. Therefore, the goal of this compendium is to provide the background information necessary to design and conduct reproducible EV FCM experiments. This compendium contains background information on EVs, the interaction between light and EVs, FCM hardware, experimental design and preanalytical procedures, sample preparation, assay controls, instrument data acquisition and calibration, EV characterization, and data reporting. Although this compendium focuses on EVs, many concepts and explanations could also be applied to FCM detection of other particles within the EV size range, such as bacteria, lipoprotein particles, milk fat globules, and viruses. CA extern

Published
2023

7. MIFlowCyt-EV: a framework for standardized reporting of extracellular vesicle flow cytometry experiments

Author

Welsh, Joshua A, Van Der Pol, Edwin, Arkesteijn, Ger J A, Bremer, Michel, Brisson, Alain, Coumans, Frank, Dignat-George, Françoise, Duggan, Erika, Ghiran, Ionita, Giebel, Bernd, Görgens, André, Hendrix, An, Lacroix, Romaric, Lannigan, Joanne, Libregts, Sten F W M, Lozano-Andrés, Estefanía, Morales-Kastresana, Aizea, Robert, Stephane, De Rond, Leonie, Tertel, Tobias, Tigges, John, De Wever, Olivier, Yan, Xiaomei, Nieuwland, Rienk, Wauben, Marca H M, Nolan, John P, Jones, Jennifer C, Welsh, Joshua A, Van Der Pol, Edwin, Arkesteijn, Ger J A, Bremer, Michel, Brisson, Alain, Coumans, Frank, Dignat-George, Françoise, Duggan, Erika, Ghiran, Ionita, Giebel, Bernd, Görgens, André, Hendrix, An, Lacroix, Romaric, Lannigan, Joanne, Libregts, Sten F W M, Lozano-Andrés, Estefanía, Morales-Kastresana, Aizea, Robert, Stephane, De Rond, Leonie, Tertel, Tobias, Tigges, John, De Wever, Olivier, Yan, Xiaomei, Nieuwland, Rienk, Wauben, Marca H M, Nolan, John P, and Jones, Jennifer C

Abstract

Extracellular vesicles (EVs) are small, heterogeneous and difficult to measure. Flow cytometry (FC) is a key technology for the measurement of individual particles, but its application to the analysis of EVs and other submicron particles has presented many challenges and has produced a number of controversial results, in part due to limitations of instrument detection, lack of robust methods and ambiguities in how data should be interpreted. These complications are exacerbated by the field's lack of a robust reporting framework, and many EV-FC manuscripts include incomplete descriptions of methods and results, contain artefacts stemming from an insufficient instrument sensitivity and inappropriate experimental design and lack appropriate calibration and standardization. To address these issues, a working group (WG) of EV-FC researchers from ISEV, ISAC and ISTH, worked together as an EV-FC WG and developed a consensus framework for the minimum information that should be provided regarding EV-FC. This framework incorporates the existing Minimum Information for Studies of EVs (MISEV) guidelines and Minimum Information about a FC experiment (MIFlowCyt) standard in an EV-FC-specific reporting framework (MIFlowCyt-EV) that supports reporting of critical information related to sample staining, EV detection and measurement and experimental design in manuscripts that report EV-FC data. MIFlowCyt-EV provides a structure for sharing EV-FC results, but it does not prescribe specific protocols, as there will continue to be rapid evolution of instruments and methods for the foreseeable future. MIFlowCyt-EV accommodates this evolution, while providing information needed to evaluate and compare different approaches. Because MIFlowCyt-EV will ensure consistency in the manner of reporting of EV-FC studies, over time we expect that adoption of MIFlowCyt-EV as a standard for reporting EV- FC studies will improve the ability to quantitatively compare results from different laboratori

Published
2020

8. MIFlowCyt-EV: a framework for standardized reporting of extracellular vesicle flow cytometry experiments

Author

LS Celbiologie-Algemeen, Celbiologie, dB&C I&I, Welsh, Joshua A, Van Der Pol, Edwin, Arkesteijn, Ger J A, Bremer, Michel, Brisson, Alain, Coumans, Frank, Dignat-George, Françoise, Duggan, Erika, Ghiran, Ionita, Giebel, Bernd, Görgens, André, Hendrix, An, Lacroix, Romaric, Lannigan, Joanne, Libregts, Sten F W M, Lozano-Andrés, Estefanía, Morales-Kastresana, Aizea, Robert, Stephane, De Rond, Leonie, Tertel, Tobias, Tigges, John, De Wever, Olivier, Yan, Xiaomei, Nieuwland, Rienk, Wauben, Marca H M, Nolan, John P, Jones, Jennifer C, LS Celbiologie-Algemeen, Celbiologie, dB&C I&I, Welsh, Joshua A, Van Der Pol, Edwin, Arkesteijn, Ger J A, Bremer, Michel, Brisson, Alain, Coumans, Frank, Dignat-George, Françoise, Duggan, Erika, Ghiran, Ionita, Giebel, Bernd, Görgens, André, Hendrix, An, Lacroix, Romaric, Lannigan, Joanne, Libregts, Sten F W M, Lozano-Andrés, Estefanía, Morales-Kastresana, Aizea, Robert, Stephane, De Rond, Leonie, Tertel, Tobias, Tigges, John, De Wever, Olivier, Yan, Xiaomei, Nieuwland, Rienk, Wauben, Marca H M, Nolan, John P, and Jones, Jennifer C

Published
2020

9. Independent human mesenchymal stromal cell-derived extracellular vesicle preparations differentially affect symptoms in an advanced murine Graft-versus-Host-Disease model

Author

Madel, Rabea Julia, primary, B&oumlrger, Verena, additional, Dittrich, Robin, additional, Bremer, Michel, additional, Tertel, Tobias, additional, Ngo Thi Phuong, Nhi, additional, Babo, Hideo A., additional, Kordelas, Lambros, additional, Buer, Jan, additional, Horn, Peter A., additional, Westendorf, Astrid M., additional, Brandau, Sven, additional, Kirschning, Carsten J., additional, and Giebel, Bernd, additional

Published
2020
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10. MIFlowCyt‐EV: a framework for standardized reporting of extracellular vesicle flow cytometry experiments

Author

Welsh, Joshua A., primary, Van Der Pol, Edwin, additional, Arkesteijn, Ger J.A., additional, Bremer, Michel, additional, Brisson, Alain, additional, Coumans, Frank, additional, Dignat‐George, Françoise, additional, Duggan, Erika, additional, Ghiran, Ionita, additional, Giebel, Bernd, additional, Görgens, André, additional, Hendrix, An, additional, Lacroix, Romaric, additional, Lannigan, Joanne, additional, Libregts, Sten F.W.M., additional, Lozano‐Andrés, Estefanía, additional, Morales‐Kastresana, Aizea, additional, Robert, Stephane, additional, De Rond, Leonie, additional, Tertel, Tobias, additional, Tigges, John, additional, De Wever, Olivier, additional, Yan, Xiaomei, additional, Nieuwland, Rienk, additional, Wauben, Marca H.M., additional, Nolan, John P., additional, and Jones, Jennifer C., additional

Published
2020
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11. Optimisation of imaging flow cytometry for the analysis of single extracellular vesicles by using fluorescence-tagged vesicles as biological reference material

Author

Görgens, André, Bremer, Michel, Ferrer-Tur, Rita, Murke, Florian, Tertel, Tobias, Horn, Peter A., Thalmann, Sebastian, Welsh, Joshua A., Probst, Christine, Guerin, Coralié, Boulanger, Chantal M., Jones, Jennifer C., Hanenberg, Helmut, Erdbrügger, Uta, Lannigan, Joanne, Ricklefs, Franz L., El-Andaloussi, Samir, and Giebel, Bernd

Subjects
viruses, Medizin, standardisation, Medizinische Fakultät » Universitätsklinikum Essen » Institut für Transfusionsmedizin, exosomes, Exosomes, Submicron Particle Analysis, Imaging Flow Cytometry, Extracellular Vesicles, ddc:610, Flow cytometry, lcsh:QH573-671, reference material, lcsh:Cytology, Medizinische Fakultät » Universitätsklinikum Essen » Zentrum für Kinder- und Jugendmedizin » Klinik für Kinderheilkunde III, flow cytometry, cd63, submicron particle analysis, respiratory system, imaging flow cytometry, Cd63, Reference material, Standardisation, extracellular vesicles, microvesicles, Microvesicles, Research Article
Abstract

Extracellular vesicles (EVs) mediate targeted cellular interactions in normal and pathophysiological conditions and are increasingly recognised as potential biomarkers, therapeutic agents and drug delivery vehicles. Based on their size and biogenesis, EVs are classified as exosomes, microvesicles and apoptotic bodies. Due to overlapping size ranges and the lack of specific markers, these classes cannot yet be distinguished experimentally. Currently, it is a major challenge in the field to define robust and sensitive technological platforms being suitable to resolve EV heterogeneity, especially for small EVs (sEVs) with diameters below 200 nm, i.e. smaller microvesicles and exosomes. Most conventional flow cytometers are not suitable for the detection of particles being smaller than 300 nm, and the poor availability of defined reference materials hampers the validation of sEV analysis protocols. Following initial reports that imaging flow cytometry (IFCM) can be used for the characterisation of larger EVs, we aimed to investigate its usability for the characterisation of sEVs. This study set out to identify optimal sample preparation and instrument settings that would demonstrate the utility of this technology for the detection of single sEVs. By using CD63eGFP-labelled sEVs as a biological reference material, we were able to define and optimise IFCM acquisition and analysis parameters on an Amnis ImageStreamX MkII instrument for the detection of single sEVs. In addition, using antibody-labelling approaches, we show that IFCM facilitates robust detection of different EV and sEV subpopulations in isolated EVs, as well as unprocessed EV-containing samples. Our results indicate that fluorescently labelled sEVs as biological reference material are highly useful for the optimisation of fluorescence-based methods for sEV analysis. Finally, we propose that IFCM will help to significantly increase our ability to assess EV heterogeneity in a rigorous and reproducible manner, and facilitate the identification of specific subsets of sEVs as useful biomarkers in various diseases. OA Förderung 2019

Published
2019

12. Precipitation with polyethylene glycol followed by washing and pelleting by ultracentrifugation enriches extracellular vesicles from tissue culture supernatants in small and large scales

Author

Ludwig, Anna-Kristin, De Miroschedji, Kyra, Doeppner, Thorsten R., Boerger, Verena, Ruesing, Johannes, Rebmann, Vera, Durst, Stephan, Jansen, Soeren, Bremer, Michel, Behrmann, Elmar, Singer, Bernhard B., Jastrow, Holger, Kuhlmann, Jan Dominik, El Magraoui, Fouzi, Meyer, Helmut E., Hermann, Dirk M., Opalka, Bertram, Raunser, Stefan, Epple, Matthias, Horn, Peter A., Giebel, Bernd, Ludwig, Anna-Kristin, De Miroschedji, Kyra, Doeppner, Thorsten R., Boerger, Verena, Ruesing, Johannes, Rebmann, Vera, Durst, Stephan, Jansen, Soeren, Bremer, Michel, Behrmann, Elmar, Singer, Bernhard B., Jastrow, Holger, Kuhlmann, Jan Dominik, El Magraoui, Fouzi, Meyer, Helmut E., Hermann, Dirk M., Opalka, Bertram, Raunser, Stefan, Epple, Matthias, Horn, Peter A., and Giebel, Bernd

Abstract

Extracellular vesicles (EVs) provide a complex means of intercellular signalling between cells at local and distant sites, both within and between different organs. According to their cell-type specific signatures, EVs can function as a novel class of biomarkers for a variety of diseases, and can be used as drug-delivery vehicles. Furthermore, EVs from certain cell types exert beneficial effects in regenerative medicine and for immune modulation. Several techniques are available to harvest EVs from various body fluids or cell culture supernatants. Classically, differential centrifugation, density gradient centrifugation, size-exclusion chromatography and immunocapturing-based methods are used to harvest EVs from EV-containing liquids. Owing to limitations in the scalability of any of these methods, we designed and optimised a polyethylene glycol (PEG)based precipitation method to enrich EVs from cell culture supernatants. We demonstrate the reproducibility and scalability of this method and compared its efficacy with more classical EV-harvesting methods. We show that washing of the PEG pellet and the re-precipitation by ultracentrifugation remove a huge proportion of PEG co-precipitated molecules such as bovine serum albumine (BSA). However, supported by the results of the size exclusion chromatography, which revealed a higher purity in terms of particles per milligram protein of the obtained EV samples, PEG-prepared EV samples most likely still contain a certain percentage of other non-EV associated molecules. Since PEG-enriched EVs revealed the same therapeutic activity in an ischemic stroke model than corresponding cells, it is unlikely that such co-purified molecules negatively affect the functional properties of obtained EV samples. In summary, maybe not being the purification method of choice if molecular profiling of pure EV samples is intended, the optimised PEG protocol is a scalable and reproducible method, which can easily be adopted by laboratories equip

Published
2018

13. EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research

Author

Van Deun, Jan, Mestdagh, Pieter, Agostinis, Patrizia, Akay, Özden, Anand, Sushma, Anckaert, Jasper, Martinez, Zoraida Andreu, Baetens, Tine, Beghein, Els, Bertier, Laurence, Berx, Geert, Boere, Janneke, Boukouris, Stephanie, Bremer, Michel, Buschmann, Dominik, Byrd, James B, Casert, Clara, Cheng, Lesley, Cmoch, Anna, Daveloose, Delphine, De Smedt, Eva, Demirsoy, Seyma, Depoorter, Victoria, Dhondt, Bert, Driedonks, Tom A P, Dudek, Aleksandra, Elsharawy, Abdou, Floris, Ilaria, Foers, Andrew D, Gärtner, Kathrin, Garg, Abhishek D, Geeurickx, Edward, Gettemans, Jan, Ghazavi, Farzaneh, Giebel, Bernd, Kormelink, Tom Groot, Hancock, Grace, Helsmoortel, Hetty, Hill, Andrew F, Hyenne, Vincent, Kalra, Hina, Kim, David, Kowal, Joanna, Kraemer, Sandra, Leidinger, Petra, Leonelli, Carina, van der Grein, Susanne, van Herwijnen, Martijn J, Wauben, Marca, Zonneveld, Marijke I, EV-TRACK Consortium, t Hoen, E.N.M., dES RMSC, dB&C I&I, and LS Celbiologie-Algemeen

Abstract

We argue that the field of extracellular vesicle (EV) biology needs more transparent reporting to facilitate interpretation and replication of experiments. To achieve this, we describe EV-TRACK, a crowdsourcing knowledgebase (http://evtrack.org) that centralizes EV biology and methodology with the goal of stimulating authors, reviewers, editors and funders to put experimental guidelines into practice.

Published
2017

14. Mesenchymal stem/stromal cell-derived extracellular vesicles and their potential as novel Mesenchymal Stem/Stromal Cell-Derived Extracellular Vesicles and Their Potential as Novel Immunomodulatory Therapeutic Agents

Author

Börger, Verena, Bremer, Michel, Ferrer-Tur, Rita, Gockeln, Lena, Stambouli, Oumaima, Becic, Amina, and Giebel, Bernd

Subjects
Medizin
Abstract

Extracellular vesicles (EVs), such as exosomes and microvesicles, have been identified as mediators of a newly-discovered intercellular communication system. They are essential signaling mediators in various physiological and pathophysiological processes. Depending on their origin, they fulfill different functions. EVs of mesenchymal stem/stromal cells (MSCs) have been found to promote comparable therapeutic activities as MSCs themselves. In a variety of in vivo models, it has been observed that they suppress pro-inflammatory processes and reduce oxidative stress and fibrosis. By switching pro-inflammatory into tolerogenic immune responses, MSC-EVs very likely promote tissue regeneration by creating a pro-regenerative environment allowing endogenous stem and progenitor cells to successfully repair affected tissues. Accordingly, MSC-EVs provide a novel, very promising therapeutic agent, which has already been successfully applied to humans. However, the MSC-EV production process has not been standardized, yet. Indeed, a collection of different protocols has been used for the MSC-EV production, characterization and application. By focusing on kidney, heart, liver and brain injuries, we have reviewed the major outcomes of published MSC-EV in vivo studies. OA gold - CA Börger

Published
2017

15. High‐Resolution Imaging Flow Cytometry Reveals Impact of Incubation Temperature on Labeling of Extracellular Vesicles with Antibodies.

Author

Tertel, Tobias, Bremer, Michel, Maire, Cecile, Lamszus, Katrin, Peine, Sven, Jawad, Rim, Andaloussi, Samir E.L., Giebel, Bernd, Ricklefs, Franz L., and Görgens, André

Abstract

Extracellular vesicles (EVs) are released from basically all cells. Over the last decade, small EVs (sEVs; 50–150 nm) have gained enormous attention in diagnostics and therapy. However, methodological limitations coupled to the lack of EV standards leave many questions in this quickly evolving field unresolved. Recently, by using enhanced green fluorescent protein (eGFP)‐labeled sEVs as biological reference material, we systematically optimized imaging flow cytometry for single sEV analysis. Furthermore, we showed that sEVs stained with different fluorescent antibodies can be analyzed in a multiparametric manner. However, many parameters potentially affecting the sEV staining procedure still require further evaluation and optimization. Here, we present a concise, systematic evaluation of the impact of the incubation temperature (4°C, room temperature and 37°C) during sEV antibody staining on the outcome of experiments involving the staining of EVs with fluorescence‐conjugated antibodies. We provide evidence that both the staining intensity and the sample recovery can vary depending on the incubation temperature applied, and that observed differences are less pronounced following prolonged incubation times. In addition, this study can serve as an application‐specific example of parameter evaluation in EV flow cytometry. © 2020 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry. [ABSTRACT FROM AUTHOR]

Published
2020
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16. Precipitation with polyethylene glycol followed by washing and pelleting by ultracentrifugation enriches extracellular vesicles from tissue culture supernatants in small and large scales

Author

Ludwig, Anna-Kristin, primary, De Miroschedji, Kyra, additional, Doeppner, Thorsten R., additional, Börger, Verena, additional, Ruesing, Johannes, additional, Rebmann, Vera, additional, Durst, Stephan, additional, Jansen, Sören, additional, Bremer, Michel, additional, Behrmann, Elmar, additional, Singer, Bernhard B., additional, Jastrow, Holger, additional, Kuhlmann, Jan Dominik, additional, El Magraoui, Fouzi, additional, Meyer, Helmut E., additional, Hermann, Dirk M., additional, Opalka, Bertram, additional, Raunser, Stefan, additional, Epple, Matthias, additional, Horn, Peter A., additional, and Giebel, Bernd, additional

Published
2018
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17. Systematic Methodological Evaluation of a Multiplex Bead-Based Flow Cytometry Assay for Detection of Extracellular Vesicle Surface Signatures

Author

Wiklander, Oscar P. B., primary, Bostancioglu, R. Beklem, additional, Welsh, Joshua A., additional, Zickler, Antje M., additional, Murke, Florian, additional, Corso, Giulia, additional, Felldin, Ulrika, additional, Hagey, Daniel W., additional, Evertsson, Björn, additional, Liang, Xiu-Ming, additional, Gustafsson, Manuela O., additional, Mohammad, Dara K., additional, Wiek, Constanze, additional, Hanenberg, Helmut, additional, Bremer, Michel, additional, Gupta, Dhanu, additional, Björnstedt, Mikael, additional, Giebel, Bernd, additional, Nordin, Joel Z., additional, Jones, Jennifer C., additional, EL Andaloussi, Samir, additional, and Görgens, André, additional

Published
2018
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18. EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research

Author

dES RMSC, dB&C I&I, LS Celbiologie-Algemeen, Van Deun, Jan, Mestdagh, Pieter, Agostinis, Patrizia, Akay, Özden, Anand, Sushma, Anckaert, Jasper, Martinez, Zoraida Andreu, Baetens, Tine, Beghein, Els, Bertier, Laurence, Berx, Geert, Boere, Janneke, Boukouris, Stephanie, Bremer, Michel, Buschmann, Dominik, Byrd, James B, Casert, Clara, Cheng, Lesley, Cmoch, Anna, Daveloose, Delphine, De Smedt, Eva, Demirsoy, Seyma, Depoorter, Victoria, Dhondt, Bert, Driedonks, Tom A P, Dudek, Aleksandra, Elsharawy, Abdou, Floris, Ilaria, Foers, Andrew D, Gärtner, Kathrin, Garg, Abhishek D, Geeurickx, Edward, Gettemans, Jan, Ghazavi, Farzaneh, Giebel, Bernd, Kormelink, Tom Groot, Hancock, Grace, Helsmoortel, Hetty, Hill, Andrew F, Hyenne, Vincent, Kalra, Hina, Kim, David, Kowal, Joanna, Kraemer, Sandra, Leidinger, Petra, Leonelli, Carina, van der Grein, Susanne, van Herwijnen, Martijn J, Wauben, Marca, Zonneveld, Marijke I, EV-TRACK Consortium, t Hoen, E.N.M., dES RMSC, dB&C I&I, LS Celbiologie-Algemeen, Van Deun, Jan, Mestdagh, Pieter, Agostinis, Patrizia, Akay, Özden, Anand, Sushma, Anckaert, Jasper, Martinez, Zoraida Andreu, Baetens, Tine, Beghein, Els, Bertier, Laurence, Berx, Geert, Boere, Janneke, Boukouris, Stephanie, Bremer, Michel, Buschmann, Dominik, Byrd, James B, Casert, Clara, Cheng, Lesley, Cmoch, Anna, Daveloose, Delphine, De Smedt, Eva, Demirsoy, Seyma, Depoorter, Victoria, Dhondt, Bert, Driedonks, Tom A P, Dudek, Aleksandra, Elsharawy, Abdou, Floris, Ilaria, Foers, Andrew D, Gärtner, Kathrin, Garg, Abhishek D, Geeurickx, Edward, Gettemans, Jan, Ghazavi, Farzaneh, Giebel, Bernd, Kormelink, Tom Groot, Hancock, Grace, Helsmoortel, Hetty, Hill, Andrew F, Hyenne, Vincent, Kalra, Hina, Kim, David, Kowal, Joanna, Kraemer, Sandra, Leidinger, Petra, Leonelli, Carina, van der Grein, Susanne, van Herwijnen, Martijn J, Wauben, Marca, Zonneveld, Marijke I, EV-TRACK Consortium, and t Hoen, E.N.M.

Published
2017

20. EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research.

Author

Van Deun J, Mestdagh P, Agostinis P, Akay Ö, Anand S, Anckaert J, Martinez ZA, Baetens T, Beghein E, Bertier L, Berx G, Boere J, Boukouris S, Bremer M, Buschmann D, Byrd JB, Casert C, Cheng L, Cmoch A, Daveloose D, De Smedt E, Demirsoy S, Depoorter V, Dhondt B, Driedonks TA, Dudek A, Elsharawy A, Floris I, Foers AD, Gärtner K, Garg AD, Geeurickx E, Gettemans J, Ghazavi F, Giebel B, Kormelink TG, Hancock G, Helsmoortel H, Hill AF, Hyenne V, Kalra H, Kim D, Kowal J, Kraemer S, Leidinger P, Leonelli C, Liang Y, Lippens L, Liu S, Lo Cicero A, Martin S, Mathivanan S, Mathiyalagan P, Matusek T, Milani G, Monguió-Tortajada M, Mus LM, Muth DC, Németh A, Nolte-'t Hoen EN, O'Driscoll L, Palmulli R, Pfaffl MW, Primdal-Bengtson B, Romano E, Rousseau Q, Sahoo S, Sampaio N, Samuel M, Scicluna B, Soen B, Steels A, Swinnen JV, Takatalo M, Thaminy S, Théry C, Tulkens J, Van Audenhove I, van der Grein S, Van Goethem A, van Herwijnen MJ, Van Niel G, Van Roy N, Van Vliet AR, Vandamme N, Vanhauwaert S, Vergauwen G, Verweij F, Wallaert A, Wauben M, Witwer KW, Zonneveld MI, De Wever O, Vandesompele J, and Hendrix A

Subjects
Biomedical Research, Databases, Bibliographic, Extracellular Vesicles physiology, Internationality
Abstract

We argue that the field of extracellular vesicle (EV) biology needs more transparent reporting to facilitate interpretation and replication of experiments. To achieve this, we describe EV-TRACK, a crowdsourcing knowledgebase (http://evtrack.org) that centralizes EV biology and methodology with the goal of stimulating authors, reviewers, editors and funders to put experimental guidelines into practice.

Published
2017
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