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11 results on '"Breiksa, Austra"'

2. Digital spatial profiling of RNA in pancreatic neuroendocrine tumors highlights the distinct profile of α-SMA positive stroma and its impact on surrounding tumor cells

Author

Niedra, Helvijs, primary, Peculis, Raitis, additional, Saksis, Rihards, additional, Vilisova, Sofija, additional, Breiksa, Austra, additional, Gerina-Berzina, Aija, additional, Earl, Julie, additional, Ruz-Caracuel, Ignacio, additional, Rosas, Marta G., additional, and Rovite, Vita, additional

Published
2023
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4. Genome wide analysis of circulating miRNAs in growth hormone secreting pituitary neuroendocrine tumor patients’ plasma

Author

Niedra, Helvijs, Peculis, Raitis, Litvina, Helena Daiga, Megnis, Kaspars, Mandrika, Ilona, Balcere, Inga, Romanovs, Mihails, Steina, Liva, Stukens, Janis, Breiksa, Austra, Nazarovs, Jurijs, Sokolovska, Jelizaveta, Liutkeviciene, Rasa, Vilkevicute, Alvita, Konrade, Ilze, and Rovite, Vita

Subjects
Cancer Research, Oncology
Abstract

BackgroundCirculating plasma miRNAs have been increasingly studied in the field of pituitary neuroendocrine tumor (PitNET) research. Our aim was to discover circulating plasma miRNAs species associated with growth hormone (GH) secreting PitNETs versus assess how the plasma levels of discovered miRNA candidates are impacted by SSA therapy and whether there is a difference in their levels between GH secreting PitNETs versus other PitNET types and healthy individuals.DesignWe compared plasma miRNA content and levels before and after surgery focusing on GH secreting PitNET patients. Selected miRNA candidates from our data and literature were then tested in a longitudinal manner in somatostatin analogues (SSA) treatment group. Additionally, we validated selected targets in an independent GH secreting PitNET group.MethodsmiRNA candidates were discovered using the whole miRNA sequencing approach and differential expression analysis. Selected miRNAs were then analyzed using real-time polymerase chain reaction (qPCR).ResultsWhole miRNA sequencing discovered a total of 16 differentially expressed miRNAs (DEMs) in GH secreting PitNET patients’ plasma 24 hours after surgery and 19 DEMs between GH secreting PitNET patients’ plasma and non-functioning (NF) PitNET patients’ plasma. Seven miRNAs were selected for further testing of which miR-625-5p, miR-503-5p miR-181a-2-3p and miR-130b-3p showed a significant downregulation in plasma after 1 month of SSA treatment. mir-625-5p was found to be significantly downregulated in plasma of GH secreting PitNET patients vs. NF PitNET patients. miR-625-5p alongside miR-130b-3p were also found to be downregulated in GH PitNETs compared to healthy individuals.ConclusionsOur study suggests that expression of plasma miRNAs miR-625-5p, miR-503-5p miR-181a-2-3p and miR-130b-3p in GH secreting PitNETs is affected by SSA treatment. Additionally, miR-625-5p can distinguish GH secreting PitNETs from other PitNET types and healthy controls warranting further research on these miRNAs for treatment efficacy.

Published
2022

6. Medication for Acromegaly Reduces Expression of MUC16, MACC1 and GRHL2 in Pituitary Neuroendocrine Tumour Tissue

Author

Saksis, Rihards, primary, Silamikelis, Ivars, additional, Laksa, Pola, additional, Megnis, Kaspars, additional, Peculis, Raitis, additional, Mandrika, Ilona, additional, Rogoza, Olesja, additional, Petrovska, Ramona, additional, Balcere, Inga, additional, Konrade, Ilze, additional, Steina, Liva, additional, Stukens, Janis, additional, Breiksa, Austra, additional, Nazarovs, Jurijs, additional, Sokolovska, Jelizaveta, additional, Pirags, Valdis, additional, Klovins, Janis, additional, and Rovite, Vita, additional

Published
2021
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7. Additional file 1 of Case report: recurrent pituitary adenoma has increased load of somatic variants

Author

Peculis, Raitis, Balcere, Inga, Radovica-Spalvina, Ilze, Konrade, Ilze, Caune, Olivija, Megnis, Kaspars, Rovite, Vita, Stukens, Janis, Nazarovs, Jurijs, Breiksa, Austra, Kiecis, Aigars, Silamikelis, Ivars, Pirags, Valdis, and Klovins, Janis

Abstract

Additional file 1. Timeline following the CARE guidelines. Additional clinical data. A detailed description of Materials and Methods used in the study.

Published
2020
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8. Additional file 3 of Case report: recurrent pituitary adenoma has increased load of somatic variants

Author

Peculis, Raitis, Balcere, Inga, Radovica-Spalvina, Ilze, Konrade, Ilze, Caune, Olivija, Megnis, Kaspars, Rovite, Vita, Stukens, Janis, Nazarovs, Jurijs, Breiksa, Austra, Kiecis, Aigars, Silamikelis, Ivars, Pirags, Valdis, and Klovins, Janis

Subjects
Data_FILES
Abstract

Additional file 3. Document file (30 pages) describing alignment quality metrics for each sample (10 pages per sample).

Published
2020
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9. Evaluation of the Possibility to Detect Circulating Tumor DNA From Pituitary Adenoma

Author

Megnis, Kaspars, primary, Peculis, Raitis, additional, Rovite, Vita, additional, Laksa, Pola, additional, Niedra, Helvijs, additional, Balcere, Inga, additional, Caune, Olivija, additional, Breiksa, Austra, additional, Nazarovs, Jurijs, additional, Stukens, Janis, additional, Konrade, Ilze, additional, Pirags, Valdis, additional, and Klovins, Janis, additional

Published
2019
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10. Exome analysis of pituitary adenoma tissue derived cell cultures

Author

Rovite, Vita, primary, Peculis, Raitis, additional, Mandrika, Ilona, additional, Petrovska, Ramona, additional, Megnis, Kaspars, additional, Balcere, Inga, additional, Konrade, Ilze, additional, Stukens, Janis, additional, Breiksa, Austra, additional, Nazarovs, Jurijs, additional, Pirags, Valdis, additional, and Klovins, Janis, additional

Published
2019
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11. Tumor and α‐SMA‐expressing stromal cells in pancreatic neuroendocrine tumors have a distinct RNA profile depending on tumor grade.

Author

Niedra, Helvijs, Peculis, Raitis, Saksis, Rihards, Mandrika, Ilona, Vilisova, Sofija, Nazarovs, Jurijs, Breiksa, Austra, Gerina, Aija, Earl, Julie, Ruz‐Caracuel, Ignacio, Rosas, Marta Gabriela, Pukitis, Aldis, Senterjakova, Natalja, and Rovite, Vita

Subjects
*GENE expression profiling, *STROMAL cells, *GENE expression, *PANCREATIC tumors, *NEUROENDOCRINE tumors
Abstract

Alpha‐smooth muscle actin (α‐SMA) expression in the stroma is linked to the presence of cancer‐associated fibroblasts and is known to correlate with worse outcomes in various tumors. In this study, using a GeoMx digital spatial profiling approach, we characterized the gene expression of the tumor and α‐SMA‐expressing stromal cell compartments in pancreatic neuroendocrine tumors (PanNETs). The profiling was performed on tissues from eight retrospective cases (three grade 1, four grade 2, and one grade 3). Selected regions of interest were segmented geometrically based on tissue morphology and fluorescent signals from synaptophysin and α‐SMA markers. The α‐SMA‐expressing stromal‐cell‐associated genes were involved in pathways of extracellular matrix modification, whereas, in tumor cells, the gene expression profiles were associated with pathways involved in cell proliferation. The comparison of gene expression profiles across all three PanNET grades revealed that the differences between grades are not only present at the level of the tumor but also in the α‐SMA‐expressing stromal cells. Furthermore, the tumor cells from regions with a rich presence of adjacent α‐SMA‐expressing stromal cells revealed an upregulation of matrix metalloproteinase‐9 (MMP9) expression in grade 3 tumors. This study provides an in‐depth characterization of gene expression profiles in α‐SMA‐expressing stromal and tumor cells, and outlines potential crosstalk mechanisms. [ABSTRACT FROM AUTHOR]

Published
2024
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