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Dryland crops rely on precipitation as their exclusive water source, but storm water runoff decreases the amount available for biomass and grain production. Management practices that minimize runoff often increase the opportunity time for infiltration and improve precipitation storage as soil water. Our objective was to quantify storm water runoff from contour farmed terraces in relation to precipitation and field characteristics and then relate runoff to soil water at planting and yields of wheat (Triticum aestivum L.) and sorghum [Sorghum bicolor (L.) Moench] grown in a 3‐yr wheat–sorghum–fallow (WSF) rotation. Comparisons of long‐term (1984–2010) runoff observations indicated mean annual runoff for both uncropped periods of the WSF rotation were significantly greater with no‐tillage (NT) than stubble‐mulch (SM) tillage. By contrast, soil loss was greater for SM than NT because residue cover of undisturbed NT soil was less subject to entrainment and loss. Increasing field slope from 1.2 to 1.8% produced greater fallow runoff, which is water lost from dryland production. Nevertheless, soil water was significantly greater with NT, which reduced evaporative losses during fallow compared with SM. Long‐term wheat yields exhibited no difference due to tillage, but NT grain sorghum yields increased significantly over SM tillage. The data show that while runoff with NT was typically greater than SM, profile soil water at planting was also greater for NT and increased grain sorghum yields significantly. We conclude that decreasing evaporative water losses with NT residues is the primary factor affecting soil water conservation. [ABSTRACT FROM AUTHOR]

Dryland crops rely on precipitation as their exclusive water source, but storm water runoff decreases the amount available for biomass and grain production. Management practices that minimize runoff often increase the opportunity time for infiltration and improve precipitation storage as soil water. Our objective was to quantify storm water runoff from contour farmed terraces in relation to precipitation and field characteristics and then relate runoff to soil water at planting and yields of wheat (Triticum aestivum L.) and sorghum [Sorghum bicolor (L.) Moench] grown in a 3‐yr wheat–sorghum–fallow (WSF) rotation. Comparisons of long‐term (1984–2010) runoff observations indicated mean annual runoff for both uncropped periods of the WSF rotation were significantly greater with no‐tillage (NT) than stubble‐mulch (SM) tillage. By contrast, soil loss was greater for SM than NT because residue cover of undisturbed NT soil was less subject to entrainment and loss. Increasing field slope from 1.2 to 1.8% produced greater fallow runoff, which is water lost from dryland production. Nevertheless, soil water was significantly greater with NT, which reduced evaporative losses during fallow compared with SM. Long‐term wheat yields exhibited no difference due to tillage, but NT grain sorghum yields increased significantly over SM tillage. The data show that while runoff with NT was typically greater than SM, profile soil water at planting was also greater for NT and increased grain sorghum yields significantly. We conclude that decreasing evaporative water losses with NT residues is the primary factor affecting soil water conservation. [ABSTRACT FROM AUTHOR]

In 1987-1989, the first irrigated crops were grown on the four large, precision weighing lysimeters at the USDA-ARS Conservation and Production Research Laboratory at Bushland, Texas, on the Southern High Plains (SHP). Thus began >25-years of full- and deficit-irrigated crop growth, energy and water balance, evapotranspiration (ET), yield, and water use efficiency (WUE) studies of major SHP crops, including alfalfa, corn and sorghum for both grain and forage, cotton, soybean, sunflower, and winter wheat. Alfalfa studies supported development of the ASCE Standardized Reference ET methodology. The lysimeter effort, led by Terry Howell, Sr., co-designed with Lynne Ebling and Thomas Marek and constructed by Arland Schneider, eventually grew to include a separate lysimeter to study short grass ET, again for the ASCE standard, and a 48-lysimeter facility to study soil type effects on crop water uptake, ET, and WUE using monoliths of four soils typical of SHP irrigated soils. The large lysimeters were managed to be representative of sprinkler-irrigated fields so as to develop crop coefficients used for irrigation scheduling by clients of ET networks developed by Texas A&M AgriLife in collaboration with the USDA-ARS. In addition, the lysimeters were used to test and further develop several technologies important to irrigation science, including soil water sensors, eddy covariance and Bowen ratio systems, scintillometers, thermal remote sensing based ET models, and hydrologic and crop simulation models. With the installation of subsurface drip irrigation systems on two of the lysimeter fields in 2013, the Bushland lysimeters are entering a new phase of advanced irrigation method and management studies. [ABSTRACT FROM AUTHOR]

Nutrient management on US dairy farms must balance an array of priorities, some of which conflict. To illustrate nutrient management challenges and opportunities across the US dairy industry, the USDA Agricultural Research Service Dairy Agroecosystems Working Group (DAWG) modeled 8 confinement and 2 grazing operations in the 7 largest US dairy-producing states using the Integrated Farm System Model (IFSM). Opportunities existed across all of the dairies studied to increase on-farm feed production and lower purchased feed bills, most notably on large dairies (>1,000 cows) with the highest herd densities. Purchased feed accounted for 18 to 44% of large dairies' total operating costs compared with 7 to 14% on small dairies (<300 milk cows) due to lower stocking rates. For dairies with larger land bases, in addition to a reduction in environmental impact, financial incentives exist to promote prudent nutrient management practices by substituting manure nutrients or legume nutrients for purchased fertilizers. Environmental priorities varied regionally and were principally tied to facility management for dry-lot dairies of the semi-arid western United States (ammonia-N emissions), to manure handling and application for humid midwestern and eastern US dairies (nitrate-N leaching and P runoff), and pasture management for dairies with significant grazing components (nitrous oxide emissions). Many of the nutrient management challenges identified by DAWG are beyond slight modifications in management and require coordinated solutions to ensure an environmentally and economically sustainable US dairy industry., (The Authors. Published by FASS Inc. and Elsevier Inc. on behalf of the American Dairy Science Association®. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).)

The objective of the experiment was to determine the effectiveness of copper oxide wire particles (COWP) in pregnant ewes and safety to lambs. COWP have been used recently as an anthelmintic in small ruminants to overcome problems associated with nematode resistance to chemical dewormers. Doses of COWP (

Several laboratories have determined the surface charge density of membranes utilizing methods based on vesicle-induced quenching of the fluorescence of 9-aminoacridine and its relief by other cations. However, the computational methods by which surface charge density were calculated have not been verified in a model system. In this study, the quenching of 9-aminoacridine fluorescence by liposomes made from varying amounts of digalactosyldiacylglyceride and phosphatidic acid and relief of quenching by salts was examined. Quenching of 9-aminoacridine fluorescence increased with increasing amounts of phosphatidic acid added, independent of the composition of the added liposomes. In certain instances, the computational methods did not yield the surface charge density of the liposomes expected from their composition. However, when the effects of background ionic strength on surface potential were considered, there was a positive correlation between expected and calculated values. Therefore, the data support the contention that changes in the fluorescence of 9-aminoacridine can be used to calculate surface charge density of membranes.

A general model for the sorption of trivalent cations to wheat-root (Triticum aestivum L cv. Scout 66) plasma membranes (PM) has been developed and includes the first published coefficients for La3+ and Al3+ binding to a biological membrane. Both ions are rhizotoxic, and the latter ion is the principal contributor to the toxicity of acidic soils around the world. The model takes into account both the electrostatic attraction and the binding of cations to the negatively charged PM surface. Ion binding is modeled as the reaction P- + IZ <==> 'PIZ-1 in which P- represents a negatively charged PM ligand, located in an estimated area of 540 A2, and IZ represents an ion of charge Z. Binding constants for the reaction were assigned for K+ (1 M-1) and Ca2+ (30 M-1) and evaluated experimentally for La3+ (2200 M-1) and H+ (21,500 M-1). Al sorption is complicated by Al3+ hydrolysis that yields hydroxoaluminum species that are also sorbed. Binding constants of 30 and 1 M-1 were assigned for AlOH2+ and Al(OH)+2, respectively, then a constant for Al3+ (20,000 m-1) was evaluated experimentally using the previously obtained values for K+, Ca2+ and H+ binding. Electrostatic attraction was modeled according to Gouy-Chapman theory. Evaluation of parameters was based upon the sorption of ions to PM vesicles suspended in solutions containing variable concentrations of H+, Ca2+ and La3+ or Al3+. Use of small volumes, and improved assay techniques, allowed the measurement of concentration depletions caused by sorption to vesicles. Some independent confirmation of our model is provided by substantial agreement between our computations and two published reports of La3+ effects upon zeta potentials of plant protoplasts. The single published report concerning the electrostatic effects of Al on cell membranes is in essential agreement with the model.

The biochemical events utilized by transport proteins to convert the chemical energy from the hydrolysis of ATP into an electro-chemical gradient are poorly understood. The inhibition of the plasma membrane ATPase from corn (Zea mays L.) roots by N-(ethoxycarbonyl)-2-ethoxy-1,2-dihydroquinoline (EEDQ) was compared to that of ATPase solubilized with N-tetradecyl-N,N-dimethyl-3-ammonio-1-propane-sulfonate (3-14) to provide insight into the minimal functional unit. The chromatographic behavior of the 3-14-solubilized ATPase activity during size exclusion chromatography and glycerol gradient centrifugation indicated that the solubilized enzyme was in a monomeric form. Both plasma membrane-bound and solubilized ATPase were inhibited by EEDQ in a time- and concentration-dependent manner consistent with a first-order reaction. When the log of the reciprocal of the half-time for inhibition was plotted as a function of the log of the EEDQ concentration, straight lines were obtained with slopes of approximately 0.5 and 1.0 for membrane-bound and 3-14-solubilized ATPase, respectively, indicating a change in the number of polypeptides per functional ATPase complex induced by solubilization with 3-14.