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11. Utilización de MALDI-TOF en el diagnóstico rápido de la sepsis

Author

Rodriguez J, Ángel Bratos M, Merino E, and Ezpeleta C

Subjects
Blood culture, Diagnóstico rápido, Espectrometría de masas, Hemocultivo, MALDI-TOF, Mass spectrometry, Rapid diagnosis, Sepsis
Abstract

The introduction of mass spectrometry through MALDI-TOF (matrix-assisted laser desorption ionization time-of-flight) in the diagnosis of bacteraemia and fungaemia has represented a revolution due to the rapidity and reliability of the results that it can offer to microbiology services and laboratories through analysis of the mass spectrum of the bacterial protein directly from positive blood culture bottles. These data are more useful if they are used in conjunction with other techniques able to identify the antibiotic resistance pattern of the microorganism. There is a need for a process of standardising sample processing protocols and for perfecting the identification of the agents causing bacteraemia, especially in some species of Gram-positive cocci and in polymicrobial processes. The introduction of this methodology provides rapid information that is highly important for the clinical management of bacteraemia. The availability of a multidisciplinary working group that applies all this information quickly and correctly in hospitals will improve the quality of care, reduce antibiotic expenditure and hospital stay and help to control the serious problem of antibiotic resistance.

Published
2016

17. Staphylococcus spp. in Spain: Present situation and evolution of antimicrobial resistance (1986-2006) | Staphylococcus spp. en España: Situación actual y evolución de la resistencia a antimicrobianos (1986-2006)

Author

Cuevas, Ó, Cercenado, E., Goyanes, M. J., Vindel, A., Trincado, P., Boqueteb, T., Marín, M., Bouza, E., Sicilia, A., Reyes, A., Herruzo, J. G., Marín, P., Ruiz, I., Calbo, L., Ramírez, J. L. D. F., Arrimadas, E., Porto, A. S., Casal, M., Del Río, M. C., Plata, C., Tejero, R., La Rosa, M., Bautista, Ma F., Maroto, C., Escobar, T., Pascual, L., Saavedra, J., Cuesta, I., Carazo, I., Molina, J. H., Pinedo, A., García, Ma V., Gallardo, M., Pascual, Á, Cueto, M., Perea, E., Aznar, J., Crespo, M. D. T., Mazuelos, E. M., López, J. L. G., Ferrero, M., Chocarro, P., Navarro, C., Torres, L., Miñana, C., Revillo, Ma J., Rezusta, A., Rubio, C., Durán, E., Vázquez, F., Aranaz, C., Santos, Ma J., Fleites, A., Lantero, M., Folgueras, I. S., Hidalgo, E., Otero, L., Miguel, Ma D., Prendes, P., Galarraga, Ma C., Sierra, G., La Iglesia, P., Ordás, J. F., Barreiro, L., Pérez, J. L., Borrell, N., Oliver, A., Gómez, J. S., Hurtado, A., Batista, N., González, I. G., Sierra, A., Gómez, Ma A. M., Martín, A. M., Hernández, A., Martínez, L. M., Campo, A. B., La Fuente, C. G., Mellado, P., Crespo, Ma D., Escribano, E., Palomar, J. J., Romero, Ma D., Solís, S., Carranza, R., Medina, C. M., Seseña, G., Bisquert, J., Tena, D., Pierna, L. D., Castañar, Ma V. M., Leturia, A. Ma, Ibáñez, R., Arroyo, R. S., Pozas, I., Ojeda, E., Mejías, G., Lizondo, C., Badía, Ma D., Gimeno, C., Junquera, S., Cachón, F., Natal, Ma I. F., Fuster, C., Alonso, E. Á, Castro, Ma A. G., Sánchez, J. E. G., Rodríguez, J. Á G., Vázquez, M. F., Carbajosa, S. G., Carrero, P., Real, S. H., Campos, Á, Betrán, A., Aldea, C., Pascual, P. P., Alberte, A., Torres, A. R., Bratos, M. Á, Lorenzo, B., Brezmes, Ma F., Urrutia, L. L., Prats, G., Anta, Ma T. J., Marco, F., Coll, P., Mirelis, B., Vilamala, A., San José, C., Martín, R., Tubau, F., Salvadó, M., González, A., Fontanals, D. D., Lite, J., Corcoy, F., Angrill, R., Morta, M., Estivill, D., Urcuola, Ma L., Surroca, J. D. B., Motje, M., Nogues, A., García, M., Santamaría, J. Ma, Gómez, F., Tapiol, J., Barba, J. L., Blanco, J., Teno, P., Viñuelas, J., Villanueva, R., González, A. F., Jove, M. R., Agulla, A., Mayo, M. R., Regueiro, B., Pardo, F., Alonso, P., Coira, A., Esteban, G., Pérez, B. F., García, P. Á, Del Blanco, T. G., Otero, I., Torres, J., Vasallo, F. J., Sevillano, P. J., Conde, I. R., Borque, L., Olarte, I., Ugalde, E., Gutiérrez, A., Perea, A. G., Otero, J. R., Chaves, F., Gadea, I., Roblas, R. F., Hervás, F., Buezas, Ma V., Santana, P. S., Picazo, J. J., Merino, P., Baquero, F., Morosini, Ma I., Pazos, C., Baquero, M., Enríquez, A. Ma, Dámaso, D., Romero, I. S., Brea, M. L., Alarcón, T., Cortés, R., Portús, Ma V., Urmeneta, A., Beltrán, M., González, R., Garcés, J. L. G., Vargas, L., Burillo, A., Aznar, E., Sánchez, A., Rico, A., Wilhelmi, I., Criado, C. G., Delgado, A., Valverde, J., Alós, J. I., Concheiro, M. S., Galán, Ma I., Sánchez, J. A. T., Ramírez, C., Guerrero, C., Segovia, M., Yagüe, G., Menasalvas, A., Artola, V. M., Beristain, X., Pina, C., Fontaneda, A., Dorronsoro, I., Irure, J. J. G., José Leiva, Hernáez, S., Trallero, E. P., Arenzana, J. Ma G., Alfaro, J. A. J., Redondo, B., Arteche, L., Iturzaeta, A., Aguinaga, A., Cisterna, R., Ibarra, K., Calvo, F., Corral, I., Elorduy, L., Rienda, I. M., Goikoetxea, Ma J. L., Michaus, L., Perales, A., Labora, A., Canut, A., Plazas, J., López, M. A., La Tabla, V. O., Zorraquino, A., Gonzalo, N., Sánchez, V., Fuentes, E., Royo, G., López, P., Moreno, R., Del Busto, A. G., Yagüe, A., Gobernado, M., Hontangas, J. L. L., Lomas, J. G., Tormo, N., Lozano, T. G., Maiquez, J., Nogueira, J. M., Canós, M., Llucián, Ma R., Lloret, A. Ma, Bosque, M., Guerrero, A., Colomina, J., Aguayo, J. Ma G., Miguel, L. A., Jiménez, Ma C. A., Ferreruela, R. Ma, Tintorer, J. L. H., Vírseda, I., Fornells, J. P., Escoms, R., and Giner, S.

18. Evolution of the antimicrobial resistance of Pseudomonas aeruginosa in Spain: Second National Study (2003)

Author

Sánchez-Romero, I., Cercenado, E., Cuevas, O., García-Escribano, N., García-Martínez, J., Bouza, E., Rodríguez-Jove, M., Álvarez, A., Agulla, J. A., Rodríguez-Mayo, M., Regueiro, B., Pardo, F., Alonso, P., Coira, A., Tinajas, A., Pulian, V., García-Campello, M., González-Blanco, T., Otero, I., Torres, J., Vasallo, F. J., Sevillano, J., Rodríguez-Conde, I., Vázquez, F., Aranaz, C., Méndez, F., Lantero, M., Hidalgo, E., Viejo, G., Miguel, M. D., Prendes, P., Rodríguez-Álvarez, J., Cimadevilla, R., Torreblanca, A., Martinez, L., Calvo, J., Colomo, L. F., Mellado, P., Cisterna, R., Ibarra, K., Calvo, F., Marzana, I., Martín-Saco, G., Alkorta, M., Barrón, J., López-Goikoetxea, M. J., Michaus, L., Pablos, M., Labora, A., Canut, A., Pérez-Trallero, E., García-Arenzana, J. M., Jiménez-Alfaro, J. A., Jauregui, A., Torroba, L., Pina, C., Fontaneda, A., Dorronsoro, I., García-Irure, J. J., Díaz-García, R., Leiva, J., Gastañares, M. J., Olarte, I., Jiménez-Anta, M. T., Marco, F., Pere Coll, Mirelis, B., Martín, R., Tubau, F., Prats, G., Larrosa, N., Salvadó, M., Fontanals, D., Mariscal, D., Lite, J., Ausina, V., Matas, L., Corcoy, F., Angrill, R., Urcula, M. L., Batlle, J., Motje, M., García-Busto, A., Moreno, R., Canós, M., Vila, B., Gobernado, M., López-Hontangas, J. L., García-Lomas, J., Navarro, D., Lloret, A., Bosque, M., Maiquez, J., Aznar, E., Nogueira, J. M., Morales, A., Llucián, M. R., García-Aguayo, J. M., Alonso, M. C., Andreu, L., González-Granda, D., Hernández, J. L., Prat, J., Escoms, R., Giner, S., Yagüe, A., Gonzalo, N., Royo, G., Cebrian, L., Altuna, A., Segovia, M., Menasalvas, A., Piqueras, J., Martínez, L., Sicilia, J. M., Ruiz, J., Vilar, V., Pérez, J. L., Borrell, N., Oliver, A., Sánchez-Gómez, J., Gutiérrez, A., García-Perea, A., Rodríguez-Otero, J. J., Chaves, F., Menéndez-Rivas, M., Hervas, F., Sánchez, P., Picazo, J. J., San Pedro, A., Baquero, F., Cantón, R., Blanco, M. A., Pazos, C., Dámaso, D., López-Brea, M., Alarcón, T., Cortés, R., Portús, V., Urmeneta, A., Beltrán, M., Gómez, P., Gómez, J. L., Tamayo, J., Wilhelmi-Cal, I., Sánchez, M., Cacho, J., Rollán, E., Pérez, T., Miñiana, C., Revillo, M. J., Rubio, C., Escartín, R., Ferrero, M., Chocarro, P., Navarro, C., Nebreda, T., Díaz, L., Brea, S., Leturia, A. M., González-Rodríguez, J. C., Barba, I., Romero, M. D., Mora, F., Bisquert, J., Pérez, M. T., Crespo, D., Escribano, E., Robles, P., Cachón, F., Fuster, C., Brezmes, M. F., López-Urrutia, L., García-Rodríguez, J. A., García-Sánchez, J. E., García-Carbajosa, S., Carrero, P., Pozas, I., Ojeda, E., Mejías, Badía, M. D., Lizondo, C., Gimeno, C., Ibáñez, R., Gómez, A., Campos, A., Merino, F., García-Castro, M. A., Álvarez, E., Rodríguez-Torres, A., Bratos, M. A., Iñiguez, R., Teno, P., Blanco, J., Garduño, E., García-Herruzo, J., Saldarreaga, A., Martín, M., Ruiz, I., Calbo, L., Francisco, J. L., Sánchez-Porto, A., Casal, M., Ibarra, A., Rosa-Fraile, M., Miranda, C., Manchado, P., Porras, J., Cuesta, I., Carazo, C., Saavedra, J. M., Pascual, L., García-Iglesias, C., Chavez, M., Perea, E., Ramírez, E., Guerrero, Y., Aznar, J., Merino, L., Martín, E., López-Barba, J., Díaz, J., Galán, M., Tur, J. A., Batista, N., Moreno, A., Sierra, A., Cuervo, M., Gallardo, R. M., Martín, A. M., Bolaños, M., Fleites, A., Santos, M. J., Esteban, G., Fernández, B., Guerrero, A., Cuenca, M., and Ramos, P.

21. ABSCESO PERIAMIGDALINO CAUSADO POR NEISSERIA MENINGITIDIS.

Author

March, G. A., Tavárez, J. J., Benito, J. I., Bratos, M. A., and Eiros, J. M.

Subjects
*PERITONSILLAR abscess, *NEISSERIA meningitidis, *MICROORGANISMS, *MENINGITIS, *SEPSIS, *RESPIRATORY infections
Abstract

Neisseria meningitidis is a higly virulent microorganism that can cause meningitis and sepsis. This microorganism can be cultivated from the throats of asymptomatic carriers and it likely enters the circulation through the upper respiratory tract but it is infrequent that N. meningitidis causes simple infections of the upper respiratory tract. Here we present a case report of peritonsillar abscess caused by N. meningitidis. [ABSTRACT FROM AUTHOR]

Published
2012

22. Role of calcifying nanoparticle in the development of hyperplasia and vascular calcification in an animal model.

Author

Cenizo Revuelta N, Gonzalez-Fajardo JA, Bratos MA, Alvarez-Gago T, Aguirre B, and Vaquero C

Subjects
Angioplasty, Balloon, Animals, Atorvastatin, Calcifying Nanoparticles administration & dosage, Carotid Arteries metabolism, Carotid Arteries pathology, Carotid Artery Injuries blood, Carotid Artery Injuries etiology, Carotid Artery Injuries pathology, Carotid Intima-Media Thickness, Cholesterol blood, Disease Models, Animal, Heptanoic Acids pharmacology, Humans, Hydroxymethylglutaryl-CoA Reductase Inhibitors pharmacology, Hyperplasia, Male, Muscle, Smooth, Vascular drug effects, Muscle, Smooth, Vascular injuries, Muscle, Smooth, Vascular pathology, Neointima, Pyrroles pharmacology, Rabbits, Time Factors, Triglycerides blood, Vascular Calcification blood, Vascular Calcification etiology, Vascular Calcification pathology, Calcifying Nanoparticles metabolism, Carotid Artery Injuries metabolism, Muscle, Smooth, Vascular metabolism, Vascular Calcification metabolism
Abstract

Objective: Calcifying nanoparticles (NPs) have been detected recently in calcified human arterial specimens and are involved in the process of calcification. This study was designed to test the hypothesis that human-derived NPs could worsen the response to arterial endothelial injury and induce vascular calcification., Methods: The right carotid artery of 24 New Zealand rabbits was injured with an angioplasty balloon. Animals were perfused intravenously with saline (100 mL) during the experiment and divided into three groups: group-A, control; group-B, exposed to NPs (2 mL) obtained from calcified aortic valves; and group-C, exposed to NPs (2 mL) and treated postoperatively with atorvastatin (2.5 mg/kg/24 h). At 30 days, both carotid arteries were removed and examined histologically. Blood measurements were monitored during the study., Results: The intimal hyperplasia area was significantly larger in the injured right carotid artery compared with the left unoperated carotid artery in all groups. There was no significant variation in medial area between groups. Morphometrically, the intima/media ratio (IMR) was significantly higher in damaged carotids compared with controls. A significant increase of IMR was found in group-B (1.81 ± 0.41) compared with group-A (0.38 ± 0.59; p = .004) or group-C (0.89 ± 0.79; p = .035). Differences between groups C and A were not significant (p = .064). Calcifications were observed in six animals, all of which had been exposed to NPs (4 in group-B, 2 in group-C, p = .027). Plasma levels of cholesterol and triglycerides remained stable., Conclusions: This research confirms the ability of systemic inoculation of human-derived NPs to accelerate hyperplasia and stimulate calcification in localized areas of arteries previously submitted to endothelial damage, while it was harmless in healthy arteries. Atorvastatin was demonstrated to slow down this process., (Copyright © 2014 European Society for Vascular Surgery. Published by Elsevier Ltd. All rights reserved.)

Published
2014
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23. Evaluation of an immunocapture-agglutination test (Brucellacapt) for serodiagnosis of human brucellosis.

Author

Orduña A, Almaraz A, Prado A, Gutierrez MP, Garcia-Pascual A, Dueñas A, Cuervo M, Abad R, Hernández B, Lorenzo B, Bratos MA, and Torres AR

Subjects
Adolescent, Adult, Aged, Antibodies, Bacterial blood, Brucella immunology, Brucellosis microbiology, Child, Coombs Test methods, Female, Humans, Male, Middle Aged, Reproducibility of Results, Sensitivity and Specificity, Agglutination Tests methods, Brucellosis diagnosis
Abstract

We evaluated the validity and the usefulness of a new test for the diagnosis of human brucellosis based on an immunocapture-agglutination technique. A total of 315 sera from 82 patients with a diagnosis of brucellosis, 157 sera from patients in whom brucellosis was suspected but not confirmed, and 412 sera from people living in rural areas with endemic brucellosis were studied. The seroagglutination test (SAT), Coombs anti-Brucella test, and Brucellacapt test were evaluated. All the initial sera from the 82 patients proved to be positive in Brucellacapt and Coombs tests, while only 75 (91.4%) were positive in the SAT. If a >/=1/160 diagnostic threshold titer was defined for the Brucellacapt test, Coombs test, and SAT, the sensitivities were 95.1, 91.5, and 65.8%, respectively. Taking the same diagnostic threshold titer for the 157 sera from the unconfirmed but suspected patients, the specificities of the Brucellacapt, Coombs, and SAT were 81.5, 96.2, and 100%, respectively; for the 412 control sera, the specificities were 99.0, 99.8, and 100%. The diagnostic efficiency (area below the receiver operating characteristic curve) of Brucellacapt was 0.987852 (95% confidence interval [CI], 0.95109 to 0.99286), very similar to the diagnostic efficiency of the Coombs test (0.97611; 95% CI, 0.94781 to 0.99146) and higher than that of SAT (0.91013; 95% CI, 0.86649 to 0.94317). The results of the Brucellacapt test were compared with those of the Coombs test (correlation coefficient, 0.956; P = 0.000) and SAT (correlation coefficient, 0.866; P = 0.000). The study shows very good correlation between the Brucellacapt and Coombs tests, with a high concordance between titers obtained in the two tests. Nevertheless, lower correlation and concordance were found between the Brucellacapt and Coombs tests when the results for titers of >/=1/160 were compared (0.692; P = 0.000). In acute brucellosis, the Brucellacapt and Coombs tests render positive titers of >/=1/160. When the titers are lower, they increase significantly in the following 30 days, despite the evolution of SAT titers. In contrast, Brucellacapt and Coombs titers are always high (>/=1/640) in brucellosis with long evolution, whether SAT titers are higher or lower than 1/160.

Published
2000
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24. [Laboratory diagnosis and serologic course in patients with tularemia].

Author

Dueñas AI, Ortega M, Garrote I, de Frutos M, Gutiérrez P, García-Pascual A, Cuervo M, Hernández Novoa B, Bratos MA, Almaraz A, Orduña A, and Rodríguez Torres A

Subjects
Adult, Aged, Aged, 80 and over, Antibodies, Bacterial blood, Female, Humans, Male, Middle Aged, Serologic Tests, Tularemia blood, Tularemia immunology, Tularemia microbiology, Tularemia diagnosis
Abstract

Background: Tularemia was practically unknown in Spain until the end of 1997, when an epidemic outbreak was declared. This paper presents the data on microbiological diagnosis of 55 patients who suffered from tularemia., Patients and Methods: Thirty-two samples from 19 patients and 151 serum samples from 55 patients were obtained for culture. Serologic diagnosis was performed by tube sero-agglutination and microagglutination. Three types of tests were performed on all sera: Wright sero-agglutination (WSA), Coombs test against Brucella spp. and sero-agglutination against Yersinia enterocolitica O:3, Yersinia enterocolitica O:3, and Proteus OX 19., Results: F. tularensis was found in two samples (6.25%) of the 32 received. Titers > or = 1/160 were obtained in 78.2% and 74.5% of the initial sera by tube sero-agglutination and microagglutination, respectively. Correlation between the two tests was 0.80 (p < 0.001). Prozone phenomenon was observed in 59.9% of the sera, while crossed reactivity to Brucella spp. and Proteus spp. was found in 9.3% and 22.8%, respectively. No crossed reactivity was observed with Yersinia spp., Conclusions: Culture of F. tularensis has low sensitivity. The correlation obtained between tube sero-agglutination and microagglutination is good. Both techniques are useful in routine diagnosis of tularemia, although microagglutination has some advantages over tube agglutination.

Published
2000

25. Evaluation of six serological tests in diagnosis and postoperative control of pulmonary hydatid disease patients.

Author

Zarzosa MP, Orduña Domingo A, Gutiérrez P, Alonso P, Cuervo M, Prado A, Bratos MA, García-Yuste M, Ramos G, and Rodríguez Torres A

Subjects
Antibodies, Helminth blood, Echinococcosis, Pulmonary surgery, Enzyme-Linked Immunosorbent Assay, False Positive Reactions, Hemagglutination Tests, Humans, Immunoelectrophoresis, Latex Fixation Tests, Serologic Tests, Echinococcosis, Pulmonary diagnosis
Abstract

Latex agglutination (LA), passive hemagglutination (PHA), immunoelectrophoresis (IEP) and specific IgE, IgM, IgG enzyme-linked immunosorbent assay (ELISA) tests for diagnosis and postoperative follow-up of 79 patients with surgically confirmed pulmonary hydatidosis were evaluated. Specific IgG ELISA was the most sensitive test (83.5%) and the least sensitive tests were specific IgE ELISA (44.3%) and IEP (50.6%). The specificity obtained for all the serologic test was above 97% in all cases. The greatest number of false positives in all tests (except IEP) occurred in patients with Taenia saginata and Taenia solium cysticerci infestations and in patients with lymphoma and leukemia. Specific IgG ELISA demonstrated the highest negative predictive value (93.8%). No statistically significant differences (p > 0.050) were found in the sensitivity of the tests when patients with only one cyst and patients with various cysts were compared. Considering only the patients without relapse, the percentage of seropositive patients increased in all tests at 1 and 3 months after surgery. After that time the percentage of seropositive patients decreased. At 48 months after surgery all patients without relapse became negative in IEP, specific IgE ELISA, and specific IgM ELISA. The antibody titers in all seropositive patients increased during the 3 months after surgery. From these 3 months onward, antibody levels decreased in all serologic tests studied in the group of patients without relapse. The patients who had relapses during the first year after surgery presented persistently elevated antibody titers in all postoperative sera. The antibody titers of the patients who relapsed between the third and fourth years after surgery decreased progressively the third month after surgery, and increased in the serum obtained at the moment of relapse diagnosis. Our results show that persistence of elevated antibody titers in patients with pulmonary hydatidosis in the year after surgery or titer increase after a progressive decrease are indicative of relapse or reinfection.

Published
1999
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26. [Variants of hepatitis C virus in different risk groups. Comparative study of a method for genotyping and another for serotyping].

Author

Alonso P, Orduña A, San Miguel A, Gutiérrez MP, Lorenzo B, Eiros JM, Bratos MA, Cuervo M, and Rodríguez Torres A

Subjects
Blood Transfusion, Comorbidity, Enzyme-Linked Immunosorbent Assay, Genotype, Hepacivirus genetics, Hepacivirus immunology, Hepacivirus isolation & purification, Hepatitis C epidemiology, Humans, Prevalence, Prospective Studies, Renal Dialysis, Risk Factors, Seroepidemiologic Studies, Spain epidemiology, Substance Abuse, Intravenous epidemiology, Hepacivirus classification, Hepatitis C virology, Hepatitis C Antibodies blood, Polymerase Chain Reaction, RNA, Viral genetics, Serotyping methods
Abstract

Aims: The aim of this study was to know the prevalence of the different variants of HCV in the Health Care area of Monforte de Lemos (Lugo, Spain) and its distribution according to risk factors and to compare the results obtained with one genotyping and one serotyping technique., Patients and Methods: Eighty-four patients with hepatitis C were studied, 25 of whom were IVDA, 14 had received blood transfusions, 4 hemodialysis and the risk factor was unknown in 41. The antibodies against HCV were studied by second generation EIA and confirmed by an immunoblot technique. Serotyping was carried out by an ELISA test. Genotyping was undertaken with a reverse hybridation test of the amplification obtained by polymerase chain reaction prior to reverse transcription (RT-PCR)., Results and Conclusions: The genotypes most frequently observed were 1b (47.6%), 1a (20.2%) and 3 (14.3%). In the IVDA patients the genotypes 1a (40%) and 3 (24%) predominated. The 1b genotype was the most prevalent in the patients of unknown risk (68.3%) and patients with a history of blood transfusion (50%). The prevalence of the different serotypes was similar to that of the corresponding genotypes, with nearly 100% agreement. The number of untypable cases was greater in the serotyping technique (20.2%) than in the genotyping (2.4%). A greater number of mixed infections was detected with serotyping (7 cases, 8.3%) than with genotyping (1 case, 1.2%). Lesser sensitivity of the serotyping test was observed in the patients lacking anti-NS4 antibodies.

Published
1998

28. [A e year study of the electrophoretic RNA patterns of rotavirus in Valladolid].

Author

Ignacio Reguera J, Ortiz de Lejarazu R, Eirós JM, Bratos MA, Gonzalo MP, González Z, Pérez-Pascual P, and Rodríguez-Torres A

Subjects
Child, Child, Preschool, Electrophoresis, Feces microbiology, Humans, Infant, Prevalence, RNA, Viral analysis, Seasons, Spain, RNA, Viral classification, Rotavirus genetics
Abstract

Background: To study the electrophoretic RNA patterns of human rotavirus (RV) during three years in Valladolid (Spain)., Material and Methods: From october 1986 to october 1989 we have studied the electrophoretic RNA patterns of RV in 104 fecal samples. Stools were first screened by a latex agglutination test (LA) and reactive samples were confirmed by a commercial enzymeimmunoassay (EIA) and electron microscopy (EM) (negative stain). Electropherotyping was made according to conventional methods., Results: 96.2% of the samples studied yield a defined electropherotype. We have found 8 different types of patterns of genomic RV-RNA circulating in these three years. Two of them belonged to short pattern (C and E) and six to long pattern (A, B, D, F, G and H)., Conclusions: 98.0% of the genomic RNA founded were of long pattern, from which pattern A was the most prevalent (72%) during the study, without a seasonal distribution. Pattern B was found in 20% of cases, while the rest were found only occasionally.

Published
1993

29. Inhibition of the degranulation and myeloperoxidase activity of human polymorphonuclear neutrophils by Brucella melitensis.

Author

Orduña A, Orduña C, Eiros JM, Bratos MA, Gutiérrez P, Alonso P, and Rodríguez Torres A

Subjects
Brucella immunology, Brucella pathogenicity, Cytoplasmic Granules drug effects, Glucuronidase analysis, Humans, Neutrophils drug effects, Neutrophils immunology, Opsonin Proteins, Peroxidase drug effects, Phagocytosis physiology, Staphylococcus aureus immunology, Staphylococcus aureus metabolism, Brucella metabolism, Cytoplasmic Granules metabolism, Neutrophils metabolism, Peroxidase metabolism
Abstract

The degranulation and myeloperoxidase-H2O2-halide activities of human polymorphonuclear leukocytes from healthy donors were tested after co-incubation with either Brucella melitensis 16M, Staphylococcus aureus or Staphylococcus aureus in presence of lipopolysaccharide, protein fraction, native hapten and soluble fractions released at 65 degrees C from smooth strain of Brucella melitensis 16M. The degranulation and myeloperoxidase activities of polymorphonuclear leukocytes were significantly higher when co-incubated with Staphylococcus aureus than with Brucella melitensis. The presence of lipopolysaccharide, protein fraction, and native hapten did not cause significant modification of either degranulation or myeloperoxidase activities of polymorphonuclear leukocytes against Staphylococcus aureus. Soluble fraction released at 65 degrees C produced a significant reduction in the myeloperoxidase activity but did not alter the degranulation of polymorphonuclear leukocytes triggered by Staphylococcus aureus.

Published
1991

30. [Antibodies against Borrelia burgdorferi in a population group from Valladolid].

Author

Tamayo L, García-Moncó JC, Bratos MA, Orduña A, Ortiz de Lejarazu R, and Rodríguez Torres A

Subjects
Adolescent, Adult, Cross-Sectional Studies, Female, Humans, Male, Middle Aged, Spain epidemiology, Antibodies, Bacterial analysis, Borrelia burgdorferi Group immunology, Lyme Disease epidemiology
Published
1990

31. Influence of doxycycline, streptomycin, cotrimoxazole and rifamycin on the functions of normal human neutrophils against Brucella melitensis.

Author

Orduña A, Orduña C, Zarzosa P, Bratos MA, Ortiz de Lejarazu R, and Rodríguez-Torres A

Subjects
Anti-Bacterial Agents pharmacology, Blood Bactericidal Activity drug effects, Brucella melitensis drug effects, Humans, Neutrophils drug effects, Neutrophils microbiology, Brucella melitensis physiology, Doxycycline pharmacology, Neutrophils physiology, Rifamycins pharmacology, Streptomycin pharmacology, Trimethoprim, Sulfamethoxazole Drug Combination pharmacology
Published
1989

33. [Erythema chronicum migrans caused by Borrelia burgdorferi].

Author

Rodríguez Torres A, Miranda A, Quiñones PA, Pérez Oliva N, Bratos MA, Orduña A, and Crespo Pinilla J

Subjects
Adult, Female, Humans, Penicillin G Benzathine therapeutic use, Lyme Disease diagnosis, Lyme Disease drug therapy
Published
1988

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