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39 results on '"Branska, Barbora"'

26. MOESM1 of Effective continuous acetoneâ butanolâ ethanol production with full utilization of cassava by immobilized symbiotic TSH06

Author

Zhangnan Lin, Hongjuan Liu, Wu, Jing, Patakova, Petra, Branska, Barbora, and Jianan Zhang

Abstract

Additional file 1: Figure S1. Schematic diagram for three-stage continuous fermentation. Figure S2. Starch testing of cassava peel with iodine solution. Figure S3. Scanning electron microscope images of C. acetobutylicum (TSH1), B. cereus (TSH2) and TSH06.

Published
2019
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27. Additional file 4: of Transcription profiling of butanol producer Clostridium beijerinckii NRRL B-598 using RNA-Seq

Author

Sedlar, Karel, Koscova, Pavlina, Vasylkivska, Maryna, Branska, Barbora, Kolek, Jan, Kristyna Kupkova, Patakova, Petra, and Provaznik, Ivo

Subjects
fungi
Abstract

Circular plots showing average coverage of the genome by RNA-Seq reads in all six time points. The outermost and the second outermost circles represent positions of genes on the forward (red) and reverse (blue) strands respectively. The third circle (green) stands for pseudogenes. The yellow peak and shading area represents transcription greater than the average and violet lower than average. Floating window of 10,000 bp with step of 200 bp was used to render the shading area. (PDF 701 kb)

Published
2018
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29. Additional file 8: of Transcription profiling of butanol producer Clostridium beijerinckii NRRL B-598 using RNA-Seq

Author

Sedlar, Karel, Koscova, Pavlina, Vasylkivska, Maryna, Branska, Barbora, Kolek, Jan, Kristyna Kupkova, Patakova, Petra, and Provaznik, Ivo

Subjects
mental disorders, fungi, behavioral disciplines and activities, human activities
Abstract

COG functional categories of differential expressed genes. Barplots showing the number of COG categories associated with differentially expressed genes between adjacent time points. (PDF 226 kb)

Published
2018
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31. Additional file 5: of Transcription profiling of butanol producer Clostridium beijerinckii NRRL B-598 using RNA-Seq

Author

Sedlar, Karel, Koscova, Pavlina, Vasylkivska, Maryna, Branska, Barbora, Kolek, Jan, Kristyna Kupkova, Patakova, Petra, and Provaznik, Ivo

Abstract

Differential analysis of adjacent time points using MA plots. MA plots showing statistically differentially expressed genes in color. Color coding respect the color coding used in Venn diagrams in Fig. 4. (PDF 315 kb)

Published
2018
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39. Flow cytometry analysis of <italic>Clostridium beijerinckii</italic> NRRL B-598 populations exhibiting different phenotypes induced by changes in cultivation conditions.

Author

Branska, Barbora, Pechacova, Zora, Kolek, Jan, Vasylkivska, Maryna, and Patakova, Petra

Subjects
BIOMASS energy, FERMENTATION, PHENOTYPES, BIOTECHNOLOGY, FLOW cytometry, CLOSTRIDIUM acetobutylicum
Abstract

Background: Biobutanol production by clostridia via the acetone–butanol–ethanol (ABE) pathway is a promising future technology in bioenergetics , but identifying key regulatory mechanisms for this pathway is essential in order to construct industrially relevant strains with high tolerance and productivity. We have applied flow cytometric analysis to C. beijerinckii NRRL B-598 and carried out comparative screening of physiological changes in terms of viability under different cultivation conditions to determine its dependence on particular stages of the life cycle and the concentration of butanol. Results: Dual staining by propidium iodide (PI) and carboxyfluorescein diacetate (CFDA) provided separation of cells into four subpopulations with different abilities to take up PI and cleave CFDA, reflecting different physiological states. The development of a staining pattern during ABE fermentation showed an apparent decline in viability, starting at the pH shift and onset of solventogenesis, although an appreciable proportion of cells continued to proliferate. This was observed for sporulating as well as non-sporulating phenotypes at low solvent concentrations, suggesting that the increase in percentage of inactive cells was not a result of solvent toxicity or a transition from vegetative to sporulating stages. Additionally, the sporulating phenotype was challenged with butanol and cultivation with a lower starting pH was performed; in both these experiments similar trends were obtained—viability declined after the pH breakpoint, independent of the actual butanol concentration in the medium. Production characteristics of both sporulating and non-sporulating phenotypes were comparable, showing that in C. beijerinckii NRRL B-598, solventogenesis was not conditional on sporulation. Conclusion: We have shown that the decline in C. beijerinckii NRRL B-598 culture viability during ABE fermentation was not only the result of accumulated toxic metabolites, but might also be associated with a special survival strategy triggered by pH change. [ABSTRACT FROM AUTHOR]

Published
2018
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