1. Electroacupuncture reduces inflammatory damage following cerebral ischemia-reperfusion by enhancing ABCA1-mediated efferocytosis in M2 microglia.
- Author
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Liao YS, Zhang TC, Tang YQ, Yu P, Liu YN, Yuan J, and Zhao L
- Subjects
- Animals, Male, Brain Ischemia pathology, Brain Ischemia metabolism, Brain Ischemia therapy, Mice, Neurons metabolism, Neurons pathology, Disease Models, Animal, Efferocytosis, Microglia metabolism, Microglia pathology, Electroacupuncture methods, ATP Binding Cassette Transporter 1 metabolism, Reperfusion Injury pathology, Reperfusion Injury therapy, Reperfusion Injury metabolism, Phagocytosis, Inflammation pathology, Mice, Inbred C57BL
- Abstract
Ischemic stroke (IS) is a severe cerebrovascular disease with high disability and mortality rates, where the inflammatory response is crucial to its progression and prognosis. Efferocytosis, the prompt removal of dead cells, can reduce excessive inflammation after IS injury. While electroacupuncture (EA) has been shown to decrease inflammation post-ischemia/reperfusion (I/R), its link to efferocytosis is unclear. Our research identified ATP-binding cassette transporter A1 (Abca1) as a key regulator of the engulfment process of efferocytosis after IS by analyzing public datasets and validating findings in a mouse model, revealing its close ties to IS progression. We demonstrated that EA can reduce neuronal cell death and excessive inflammation caused by I/R. Furthermore, EA treatment increased Abca1 expression, prevented microglia activation, promoted M2 microglia polarization, and enhanced their ability to phagocytose injured neurons in I/R mice. This suggests that EA's modulation of efferocytosis could be a potential mechanism for reducing cerebral I/R injury, making regulators of efferocytosis steps a promising therapeutic target for EA benefits., (© 2024. The Author(s).)
- Published
- 2024
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