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The potential of C. tropicalis to colonize and infect rat tongue mucosa was demonstrated. Thirty female Sprague-Dawley rats were infected orally with three different strains of C. tropicalis. The animals were killed one and three weeks following the inoculation and sections of their tongue were stained with hematoxylin-eosin and Grocott stain. Histological changes were observed in the group of animals killed one week after inoculation and infected with C. tropicalis strain isolated from the crural ulcer of a diabetic patient. The most important finding was acute purulent myositis with the formation of abscesses. The myositis was local without signs of spreading to the surrounding tissue. Epithelium-penetrating hyphae observed in the Grocott-stained sections were relatively fewer and more sparsely distributed. [ABSTRACT FROM AUTHOR]

Cryptococcus neoformans isolated from various clinical materials in 14 cases, was identified by ( 1) cultivation on Sabouraud glucose agar and CHROMagar Candida, ( 2) microscopic examination of Indian-ink-stained preparations and ( 3) determination of biochemical properties (assimilation and fermentation of saccharides, assimilation of KNO3, production of urease and phenol monooxygenase). C. neoformans was determined in five specimens from pædiatric patients in the intensive care unit and in nine specimens from adult patients, most frequently from liquor at meningitis ( n=3). [ABSTRACT FROM AUTHOR]