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Your search keyword '"Brégeon, Damien"' showing total 19 results
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19 results on '"Brégeon, Damien"'

1. Functional redundancy in tRNA dihydrouridylation

Author

Sudol, Claudia, primary, Kilz, Lea-Marie, additional, Marchand, Virginie, additional, Thullier, Quentin, additional, Guérineau, Vincent, additional, Goyenvalle, Catherine, additional, Faivre, Bruno, additional, Toubdji, Sabrine, additional, Lombard, Murielle, additional, Jean-Jean, Olivier, additional, de Crécy-Lagard, Valérie, additional, Helm, Mark, additional, Motorin, Yuri, additional, Brégeon, Damien, additional, and Hamdane, Djemel, additional

Published
2024
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5. Dihydrouridine synthesis in tRNAs is under reductive evolution in Mollicutes

Author

Faivre, Bruno, primary, Lombard, Murielle, additional, Fakroun, Soufyan, additional, Vo, Chau-Duy-Tam, additional, Goyenvalle, Catherine, additional, Guérineau, Vincent, additional, Pecqueur, Ludovic, additional, Fontecave, Marc, additional, De Crécy-Lagard, Valérie, additional, Brégeon, Damien, additional, and Hamdane, Djemel, additional

Published
2021
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19. Exploring a unique class of flavoenzymes: Identification and biochemical characterization of ribosomal RNA dihydrouridine synthase.

Author

Toubdji S, Thullier Q, Kilz LM, Marchand V, Yuan Y, Sudol C, Goyenvalle C, Jean-Jean O, Rose S, Douthwaite S, Hardy L, Baharoglu Z, de Crécy-Lagard V, Helm M, Motorin Y, Hamdane D, and Brégeon D

Subjects
RNA, Ribosomal, 23S metabolism, RNA, Ribosomal, 23S genetics, RNA, Ribosomal, 23S chemistry, Uridine analogs & derivatives, Uridine metabolism, Uridine chemistry, Escherichia coli Proteins metabolism, Escherichia coli Proteins genetics, Escherichia coli Proteins chemistry, RNA, Bacterial metabolism, RNA, Bacterial genetics, RNA, Bacterial chemistry, Escherichia coli genetics, Escherichia coli metabolism
Abstract

Dihydrouridine (D), a prevalent and evolutionarily conserved base in the transcriptome, primarily resides in tRNAs and, to a lesser extent, in mRNAs. Notably, this modification is found at position 2449 in the Escherichia coli 23S rRNA, strategically positioned near the ribosome's peptidyl transferase site. Despite the prior identification, in E. coli genome, of three dihydrouridine synthases (DUS), a set of NADPH and FMN-dependent enzymes known for introducing D in tRNAs and mRNAs, characterization of the enzyme responsible for D2449 deposition has remained elusive. This study introduces a rapid method for detecting D in rRNA, involving reverse transcriptase-blockage at the rhodamine-labeled D2449 site, followed by PCR amplification (RhoRT-PCR). Through analysis of rRNA from diverse E. coli strains, harboring chromosomal or single-gene deletions, we pinpoint the yhiN gene as the ribosomal dihydrouridine synthase, now designated as RdsA. Biochemical characterizations uncovered RdsA as a unique class of flavoenzymes, dependent on FAD and NADH, with a complex structural topology. In vitro assays demonstrated that RdsA dihydrouridylates a short rRNA transcript mimicking the local structure of the peptidyl transferase site. This suggests an early introduction of this modification before ribosome assembly. Phylogenetic studies unveiled the widespread distribution of the yhiN gene in the bacterial kingdom, emphasizing the conservation of rRNA dihydrouridylation. In a broader context, these findings underscore nature's preference for utilizing reduced flavin in the reduction of uridines and their derivatives., Competing Interests: Competing interests statement:The authors declare no competing interest.

Published
2024
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