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Natural fossil endocasts are a direct source of information on palaeoneurology of extinct species and, in recent years, there has been a dramatic revival of interest in palaeoneurological investigations. Endocasts of bovines (family Bovidae), however, remain hitherto undescribed. Here we describe two well preserved natural endocasts of Pleistocene bovines from the Upper Siwalik deposits exposed near Panchkula (Haryana), in the vicinity of Chandigarh city, northern India. The two endocasts, referred here to cf. Bubalus platyceros and Bovini indet., are similar in gross morphology but differ significantly in several respects. Comparison with modern bovines reveals important similarities between the Siwalik endocasts and the brains of Bos taurus and Bubalus bubalis, especially in size of their cerebral hemispheres and placement of fissures. However, the Siwalik endocasts differ significantly in being less replete with gyri and sulci, suggesting that bovines have undergone an increase in complexity of their neuroanatomy during the later part of the Pleistocene. This study also supports the recently proposed hypothesis that mammalian taxa with relatively large brain size were more likely to survive the Pleistocene megafaunal extinctions. [ABSTRACT FROM AUTHOR]

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The present study was undertaken on 164 bovine milk samples (cow = 113 and Buffalo = 51) collected from various dairy farms in and around Rewa (Madhya Pradesh, India) and only 24 samples (6.83%) were found to be positive for mastitis when screened through California Mastitis Test (CMT). The prevalence of subclinical mastitis in cattle was 21%. All 17 isolates were phenotypically characterized by mannitol fermentation, biochemical tests (acetoin production by Voges-Proskauer test), beta-galactosidase test susceptibility to novobiocin (5 µ µµg disk), resistance to polymyxin B (300 µg disk). We also observed the biofilm formation ability of all the Staphylococcus aureus strains (n=17) by Congo Red Agar (CRA), Microtiter plate (using crystal violet), and light microscopy method, 90% sensitivity was seen through microtiter plate method. Antibiofilm activity of garlic oil was undertaken on positive isolates (3%, 2%, and 1% concentration) along with positive and negative control in every microtiter plate assay. Maximum inhibition was observed at 3% concentration with O.D values ranging from 0.021±0.007 to 0.291 ± 0.005 in all the samples with percent inhibition (50 to 60%). Multi-drug resistance profiles of biofilm-producing isolates were also undertaken against polymyxin, novobiocin, tetracyclines, cotrimoxazole, clindamycin, cefoxitin, and cefoperazone. Eighty-two (82) percent of isolates showed resistance against polymyxin, 52% against novobiocin and erythromycin, and all the isolates showed 100% sensitivity against tetracyclines and cefoxitin.

The present study was undertaken on 164 bovine milk samples (cow = 113 and Buffalo = 51) collected from various dairy farms in and around Rewa (Madhya Pradesh, India) and only 24 samples (6.83%) were found to be positive for mastitis when screened through California Mastitis Test (CMT). The prevalence of subclinical mastitis in cattle was 21%. All 17 isolates were phenotypically characterized by mannitol fermentation, biochemical tests (acetoin production by Voges-Proskauer test), beta-galactosidase test susceptibility to novobiocin (5 µg disk), resistance to polymyxin B (300 µg disk). We also observed the biofilm formation ability of all the Staphylococcus aureus strains (n=17) by Congo Red Agar (CRA), Microtiter plate (using crystal violet), and light microscopy method, 90% sensitivity was seen through microtiter plate method. Antibiofilm activity of garlic oil was undertaken on positive isolates (3%, 2%, and 1% concentration) along with positive and negative control in every microtiter plate assay. Maximum inhibition was observed at 3% concentration with O.D values ranging from 0.021±0.007 to 0.291 ± 0.005 in all the samples with percent inhibition (50 to 60%). Multi-drug resistance profiles of biofilm-producing isolates were also undertaken against polymyxin, novobiocin, tetracyclines, cotrimoxazole, clindamycin, cefoxitin, and cefoperazone. Eighty-two (82) percent of isolates showed resistance against polymyxin, 52% against novobiocin and erythromycin, and all the isolates showed 100% sensitivity against tetracyclines and cefoxitin. [ABSTRACT FROM AUTHOR]

Several pathotypes of enteric E. coli have been identified. The group represented by Shiga toxin-producing E. coli (STEC) is of particular interest. Raw milk and raw milk products are significant sources of STEC infection in humans; therefore, identifying pathogens at the herd level is crucial for public health. Most national surveillance programs focus solely on raw milk and raw milk cheeses that are ready for retail sale, neglecting the possibility of evaluating the source of contamination directly at the beginning of the dairy chain. To assess the viability of the application of new molecular methodologies to STEC identification in raw milk filters and in calf feces, we analyzed 290 samples from 18 different dairy herds, including 88 bulk tank milk (BTM), 104 raw milk filters (RMF), and 98 calf feces samples. In total 3.4% of BTM, 41.4% of RMF, and 73.4% of calves' feces were positive for stx, supporting our hypothesis that BTM is not a suitable matrix to assess the presence of STEC at herd level, underestimating it. Our conclusion is that the surveillance program needs critical and extensive improvements such as RMF and calves' feces analysis implementation to be more efficient in detecting and preventing STEC infections. The epidemiology of these infections and the characteristics of the pathogen clearly show how a One Health approach will be pivotal in improving our capabilities to control the spread of these infections. [ABSTRACT FROM AUTHOR]

Background: Paramphistomosis, a parasitic condition caused by Calicophoron microbothrioides in domestic ruminants, has garnered limited attention among cattle breeders in the Cajamarca Valley, Peru. Despite its status as an endemic affliction with considerable risk, scant investigations have been conducted on this parasite within this region. Objective: This study aimed to assess the correlation between the population of adult parasites (referred to as "number of adult parasites" or NAP) in rumen and reticulum of naturally infected cows and the number of eggs per gram of feces (EPG). Methods: A sample of twenty-two cattle harboring adult parasites in their rumen and reticulum was selected for analysis. Fecal samples were collected from these animals to establish a correlation between NAP and EPG. Data analysis included linear regression, the Shapiro-Wilk normality test, and the nonparametric Mann-Whitney test. Results: The study revealed a remarkably strong and directly proportional association between the number of adult parasites in rumen and reticulum and the coproparazitologic EPG (r = 0.971). Conclusion: According to the linear model (NAP = 60.381 ± 3.37; EPG - 22.979) increasing EPG by one lead to an increase by 60 NAP in rumen and reticulum (p<0.01). [ABSTRACT FROM AUTHOR]

Bioactive ingredients are secondary metabolites produced in plants and are usually extracted and used for different purposes such as rumen fermentation and product quality improvement in animal nutrition. One of the bioactive ingredients is tannin from quebracho trees which could be used in livestock nutrition. This study was performed to evaluate the effect of supplementing a finishing diet with 0.3% (w/w DM) of quebracho tannin extract on lipid oxidation in meat derived from bullocks. Sixteen crossbred bullocks (Bostaurus x Bosindicus, with 422.71 ± 6.70 kg initial weight) were randomly assigned to receive one of the two treatments during the last 40 days in the feedlot and steers were fed on the finishing diet (CTRL) or same diet added with 0.3% (DM basis) of tannin extract (TE). The bullocks were slaughtered, and after 24 h chilling (4 °C), the left half of the carcass was cross sectioned between 12 and 13th ribs, and carcass traits were measured. The samples of longissimus thoracis muscle were taken and chemical composition and fatty acid profile were measured while TBARS was evaluated during 12 display days. The results were analysed as completely randomized design and each bullock was used as an experimental unit. Feedlot performance and carcass characteristics of bullocks were not affected by tannins addition (P > 0.05). Saturated fatty acids, monounsaturated fatty acids, n-6, and n-3 polyunsaturated fatty acids were also not affected by the tannin inclusion. Tannins decreased lipid oxidation at day 12 (P < 0.01) and did not affect (P > 0.05) other meat shelf life and chemical composition variables. Product of quebracho tannin extract had no effect on the feedlot performance, carcass characteristics, fatty acid profile of beef, pH, and colour of beef of intensively fattened bullocks. Notwithstanding, the results suggest that supplementing produced tannin extract at 0.3% of dietary DM could improve bovine meat shelf life by decreasing the longissimus thoracis thiobarbituric acid reactive substance kept during 12-day storage. [ABSTRACT FROM AUTHOR]

Abstract Rhipicephalus microplus one of the ectoparasites with the most significant impact on livestock health and the economy in Ecuador and globally. Controlling ticks through chemical means has been demonstrated to be ineffective, resulting in acaricide resistance and increasing costs and health problems in tropical and subtropical livestock. Several tick control alternatives have been investigated to date, showing promising results under laboratory conditions but not in field applications. The use of multiple control alternatives in a comprehensive management plan for tick control has proven to be effective in reducing tick infestation. The objective of this study was to determine the effectiveness of immunization with the GavacⓇ vaccine and its effect on the rational use of acaricides. The study aimed to establish the impact of immunization on the biotic potential of Rhipicephalus microplus, the level of infestation, and the frequency of acaricidal baths. In a pilot farm, 176 bovines were vaccinated, and 30 animals were observed every 15 days for eight months, resulting in 18 observations. Observationally, a significant reduction in tick load on animals was identified, as well as a reduction in the use of acaricides by less than 10% (1 bath) compared to the farm's estimated use. On average, acaricidal baths were administered 23 days after vaccination. The study found a statistically significant decrease in the number of ticks on the animals, the weight of the eggs, and their hatching percentage after the inclusion of the vaccination program on the farm. However, no statistically significant effect on the weight of engorged ticks was observed. In conclusion, the GavacⓇ vaccine has a positive observational effect on controlling the presence of ticks in animals and a negative effect on their reproductive aspects.

The objective of the current study was to determine the performance of creole cows of the Chino Santandereano breed in the ultrasound-guided follicular aspiration technique (OPU) and the quality of oocytes recovered. A total of 15 multiparous cows were selected from a herd located in the department of Santander with tropical climate. The cows were submitted to 5 sessions of follicular aspiration with an interval of 45 days. In each aspiration session, 7.9 ± 0.7 oocytes were recovered per cow, with a recovery rate of 64.9% ± 0.5 and a rate of viable and non-viable oocytes of 64.3% ± 0.6 and 35.7% ± 0.7, respectively. A difference (P < 0.05) in oocyte quality was presented for grades I, II and III (7.7 ± 0.4, 19.8 ± 0.5 and 36.8 ± 0.5, respectively). The results suggest that Chino Santandereano cows submitted to the OPU technique have a higher percentage of grade III oocytes recovered. [ABSTRACT FROM AUTHOR]

Background: The left coronary artery (LCA) in the bovines is more developed than the right. Aim: The objective of the study is to describe the bovine coronary system from a morphological point of view, including the morphometry and its distribution. Methods: Arciform suture with 2.0 silk was applied around the sinus orifice and coronary ostium and a number 14 catheter was installed, to perfuse semi-synthetic polyester resin, consisting of a mixture of 85% GP40L palatal with 15% styrene with red color mineral. Results: The average weight of 28 bovine hearts used in our study was 1.534.1 kg. The right coronary artery had a proximal caliber of 5 +/- 0.9 mm. The LCA caliber and length were 9.4 +/- 1.2 and 18.3 +/- 4.8 mm, respectively. This artery was divided into two branches in 85.7% of the cases and trifurcated in 14.3%. The paraconal interventricular branch (PIB) ended more frequently in the apex (46.4%), and its proximal caliber was 6.4 +/- 1.4 mm. The left circumflex branch ended in 82.1% in the subsinusal interventricular sulcus, and its proximal caliber was 5.9 +/- 1.2 mm. The proximal calibers of the PIB and the left circumflex branch did not present statistically significant differences (p = 0.137). The sinoatrial branch presented a dual origin (right and LCA) in 46.4% of the cases and a single origin from the LCA in 53.6% of the samples. In the evaluated hearts, left coronary dominance was observed in most cases (96.4%). Their presence of anastomosis between the branches of the coronary arteries was observed in 57.1% of cases. Conclusion: The presence of a myocardial bridge was found in six hearts (21.4%). In bovines, a high percentage of anastomosis was found, a protective factor in obstruction of the coronary arterial branches. [ABSTRACT FROM AUTHOR]

The current cross-sectional study aimed to determine the seroprevalence of Leptospira infection in bovine dairy farms in the Telangana state of India, as well as the associated risk factors, in order to implement effective preventive measures for disease control. A total of 469 blood samples were collected from 67 herds/farms in different areas, covering 20 administrative districts in the state. These samples consisted of 253 from cattle and 216 from buffaloes. Questionnaires were used to collect data on host and epidemiological factors. The collected sera were tested using the gold standard serological test, the Microscopic Agglutination Test (MAT), which employed a panel of 18 reference serovars for Leptospira exposure. The statistical analysis of epidemiological data was carried out to identify the risk factors associated with Leptospira exposure. The overall observed seroprevalence at the animal and farm levels was 41.4% and 77.6%, respectively. The most prevalent anti-leptospiral antibodies were observed against the serogroups Icterohaemorrhagiae (32.4%), Pomona (22.2%), Javanica (19.1%), Australis (17.0%), Bataviae (15.5%), Autumnalis (12.9%), Hebdomadis (12.9%), and others, in the total reacting samples. At the animal level, the significant risk factors associated with exposure to Leptospira species were breed (p = 0.03) and health status (p = 0.03). Furthermore, the multivariate statistical analysis of farm factors revealed that farm size (p = 0.05), presence of dogs (p = 0.04) and rodents (p = 0.01) on the farm, use of fodder from wet soils (p = 0.04), and proximity to water bodies (p = 0.04) were significantly associated with exposure to Leptospira in the studied region. This study provides the first report from India highlighting the important risk factors at the herd/farm and animal level associated with Leptospira infections in cattle and buffaloes. The findings contribute to strengthening the one-health strategy by facilitating the design and planning of appropriate control measures to alleviate the burden of leptospirosis in bovines. [ABSTRACT FROM AUTHOR]

The post-partum reproductive performance of cows is identified as a pivotal factor influencing the success or failure of the dairy industry. Achieving timely uterine involution, complete endometrial regeneration, and the resumption of ovarian cyclic activity are crucial for conception within the desired timeframe. However, uterine contamination with pathogens during parturition remains an unavoidable challenge, leading to suboptimal productivity and fertility. Metritis and clinical endometritis are recognized post-partum conditions, while sub-clinical endometritis has emerged as a significant threat to achieving desired fertility in dairy cows. This review summarizes a comprehensive assessment of factors contributing to delayed clearance and persistence of uterine infections in dairy cows, particularly sub-clinical endometritis (SCE). Diagnostic techniques, including bacterial culture, the Whiteside test, endometrial cytology, and trans-rectal ultrasonography (TRUS), are discussed, each with advantages and limitations. The paper explores the association between SCE and various risk factors, such as negative energy balance (NEB), metabolic indicators, and hormonal changes during the post-partum period. The significance of parameters like body condition score (BCS), backfat thickness (BFT), and metabolic indicators (leptin, NEFA, BHBA) in assessing energy status and predicting reproductive efficiency is highlighted. In addition, the underexplored area of fungal infections in dairy cows' uteri, emphasizing fungi's opportunistic nature and their potential to cause reproductive failure has also been reviewed. The immune system's role in uterine defense against fungal infections and the diagnostic challenges, including mycological cultures and cytological examinations, the therapeutic challenges, and the poor prognosis associated with fungal endometritis have also been discussed. [ABSTRACT FROM AUTHOR]

Background: The left coronary artery in the bovines is more developed than the right. Aim: The objective of the study is to describe the bovine coronary system from a morphological point of view, including the morphometry and its distribution. Methods: Arciform suture with 2.0 silk was applied around the sinus orifice and coronary ostium and a number 14 catheter was installed, to perfuse semi-synthetic polyester resin, consisting of a mixture of 85% GP40L palatal with 15% styrene with red color mineral. Results: The average weight of 28 bovine hearts used in our study was 1.534.1 kg. The right coronary artery had a proximal caliber of 5 +/- 0.9 mm. The left coronary artery caliber and length were 9.4 +/- 1.2 mm and 18.3 +/- 4.8 mm, respectively. This artery was divided into two branches in 85.7% of the cases and trifurcated in 14.3%. The paraconal interventricular branch ended more frequently in the apex (46.4%), and its proximal caliber was 6.4 +/- 1.4 mm. The left circumflex branch ended in 82.1% in the subsinusal interventricular sulcus, and its proximal caliber was 5.9 +/- 1.2 mm. The proximal calibers of the paraconal interventricular branch and the left circumflex branch did not present statistically significant differences (p=0.137). The sinoatrial branch presented a dual origin (right and left coronary arteries) in 46.4% of the cases and a single origin from the left coronary artery in 53.6% of the samples. In the evaluated hearts, left coronary dominance was observed in most cases (96.4%). There presence of anastomosis between the branches of the coronary arteries was observed in 57.1% of cases. Conclusion: The presence of a myocardial bridge was found in six hearts (21.4%). In bovines, a high percentage of anastomosis was found, a protective factor in obstruction of the coronary arterial branches. [Open Vet J 2023; 13(8.000): 955-964]

Bovines are important reservoir hosts of schistosomiasis, placing humans and animals in rice fields areas at risk of infection. This study reported the prevailing infection of zoonotic parasites from bovine feces in the rice fields adjacent to Lake Mainit, Philippines. Formalin Ethyl Acetate Sedimentation was performed on 124 bovine fecal samples from rice fields and documented eggs and cysts from seven parasites: Schistosoma japonicum, Fasciola gigantica, Ascaris sp., Strongyloides sp., Balantidium coli, coccidian oocyst and a hookworm species. Among these parasites, F. gigantica harboured the highest infection with a 100% prevalence rate, followed by hookworms (51.61%), B. coli (30.64%) and S. japonicum (12.09%), respectively. The intensity of infection of S. japonicum eggs per gram (MPEG = 4.19) among bovines is categorized as ‘light.’ Bovine contamination index (BCI) calculations revealed that, on average, infected bovines in rice fields excrete 104 750 S. japonicum eggs daily. However, across all ricefield stations, bovines were heavily infected with fascioliasis with BCI at 162 700 F. gigantica eggs per day. The study reports that apart from the persistent cases of schistosomiasis in the area, bovines in these rice fields are also heavily infected with fascioliasis. The study confirms the critical role of bovines as a reservoir host for continued infection of schistosomiasis, fascioliasis and other diseases in the rice fields of Lake Mainit. Immediate intervention to manage the spread of these diseases in bovines is recommended.

Background and Aim: Subclinical mastitis (SCM) caused by erythromycin-resistant Staphylococcus aureus is a significant disease in lactating animals. Therefore, it is crucial to understand the genetic factors contributing to erythromycin resistance in S. aureus. This study aimed to estimate the prevalence of S. aureus in milk from subclinical mastitic cattle and buffaloes and tank milk samples as identified by probe-based real-time polymerase chain reaction (PCR) and the genotypic assessment of macrolide and erythromycin resistance profiles, as well as to analyze the phylogenetic relatedness of our local isolates of S. aureus. Materials and Methods: In total, 285 milk samples were analyzed using the California mastitis test to detect SCM. Milk samples were cultured on different specific Staphylococcus media. The presence of S. aureus was confirmed by Gram staining, the catalase and coagulase tests, the detection of hemolytic activity, DNase agar testing, and biofilm activity in Congo red medium. The genotypic identification of S. aureus (nuc) was performed. The determinants of erythromycin (ermA, ermB, ermC, and ermT) and macrolide resistance (msrA) were screened in all isolates. DNA sequencing of our local isolates of S. aureus was used to analyze their phylogenetic relatedness. Moreover, histopathological examination of tissue specimens of mammary gland was performed. Results: The S. aureus positivity rates were 36.4%, 48.8%, and 63.6% in cattle, buffalo, and bulk tank milk, respectively. Probe-based real-time PCR molecularly confirmed all 62 S. aureus isolates. Thirty-one isolates were subjected to PCR to create profiles of their genotypic erythromycin resistance. ermA, ermB, ermC, and ermT were present in 5 (8%), 26 (41.9%), 18 (29%), and 15 (24.1%) S. aureus isolates, respectively. Moreover, msrA was found in three (4.8%) strains. Eight PCR products were produced using standard PCR for DNA sequencing. Multiple sequence alignment, phylogenetic tree construction, and analysis of nuc in S. aureus revealed a high degree of homology (100%) with S. aureus strains isolated from milk in cases of bovine mastitis in India and Kenya. Histological analysis of udder tissues revealed extensive aggregation of mononuclear inflammatory cells in the interstitial connective tissue, primarily lymphocytes, and macrophages. Conclusion: This study showed a high prevalence of erythromycin resistance in S. aureus isolates. This information is vital for controlling mastitis and the spread of resistance genes between bacterial strains and hosts. Moreover, the probe-based real-time PCR approach is helpful for the rapid screening of S. aureus isolates and the consequent efficient treatment and control of S. aureus mastitis.

ABSTRACT Bovine tritrichomonosis is a venereal disease that causes economic losses around the world. Over the last 100 years, the life cycle of the protozoan Tritrichomonas foetus in bovines has only considered venereal transmission, almost exclusively through natural mating from an infected animal to a healthy animal. Here, we provide the first direct evidence that T. foetus can survive the passage through the gastrointestinal tract in bovines. We demonstrated that the parasite can be discharged by feces in orally infected animals and contaminate the cow’s reproductive tract. We showed that the parasite is capable of surviving at pH conditions usually found along the bovine gastrointestinal tract, and our findings suggest that the parasites could survive in bovine feces and water for several days, probably through the formation of resistant pseudocysts or cyst-like structures. Taken together, our results demonstrate that T. foetus is more resistant to adverse external conditions than previously considered, which could be relevant for the life cycle of this parasite in bovines. IMPORTANCE Nowadays, the routine herd diagnosis is usually performed exclusively on bulls, as they remain permanently infected, and prevention and control of Tritrichomonas foetus transmission are based on identifying infected animals and culling practices. The existence of other forms of transmission and the possible role of pseudocysts or cyst-like structures as resistant forms requires rethinking the current management and control of this parasitic disease in the future in some livestock regions of the world.

Photography, also an innovative technology at the time, engaged with two interconnected bovine technologies in late nineteenth and early twentieth century India: cattle as the animal power behind other agrarian technologies and cattle breeding. The Ongole cattle of Nellore district in south India, ancestors of the internationally renowned zebu cattle, were famed for their beauty, their strength as haulage and tillage animals, and their milk yields. Along with other Indian breeds, they were systematically photographed, beginning with the 1865 Addanki cattle show. This article examines the role of bovine photography as an agent of technological change in relation to the transition from a local cattle-raising culture through provincial "improvement" schemes to a pantropical market for Ongoles. It reflects on the racial discourse of animal breeds and its intersection with colonial-era ideas of bovine "degeneration" and "improvement," while highlighting the importance of cattle fairs and experimental farms. [ABSTRACT FROM AUTHOR]

The main class of nutritional interest for lipids are fatty acids (FA), which correspond to 90% of triglycerides, the main form of lipid storage in both plants and animals. FAs serve as a source of energy in the diet of cattle; however, they also have an important non-caloric effect on animal organisms as they are important components of the physical and functional structures of cells and participate in the composition of steroid hormones. As such, research has studied the improvement of semen quality through the provision of polyunsaturated FAs in bull diets, as well as the use of FAs in semen extenders in order to reduce damage to sperm cells, which can alter lipid composition and the quality of frozen sperm. Therefore, the objective of this work was to review the effectiveness of lipids on reproductive efficiency, based on their effects on semen quality and hormonal production. Supplementation with polyunsaturated FAs positively alters semen composition and in vitro fertility; however, results vary according to the type of FA used, the method of administration, and its quality. Fish oil and linseed oil showed better results in qualitative parameters in fresh and thawed semen. The use of cyclodextrins to incorporate or extract cholesterol from plasma membranes can also improve the viability of cryopreserved semen. [ABSTRACT FROM AUTHOR]

Vancomycin‐resistant Staphylococcus aureus (VRSA) is a zoonotic life‐threatening pathogen. Vancomycin exhibits anti‐bacterial activity by inhibiting peptidoglycan synthesis by binding to the D‐ala‐D‐ala terminus of the peptidoglycan. But in VRSA, D‐ala‐D‐ala is replaced by D‐ala‐D‐lactate due to the presence of vanA, vanB or vanD genes. This study was intended to identify the molecular prevalence of VRSA in 768 bovine milk samples, risk factor association, antibiogram profile and bioinformatics analysis of VRSA by targeting vanB gene. Out of a total of 248 S. aureus isolates from mastitic milk samples, the phenotypic and genotypic prevalence of VRSA was estimated to be 17.74% and 10.89%, respectively. Farm‐level risk factors including use of improper milking technique, lack of milker's care during milking, unhygienic conditions during milking and no dry cow therapy were found to be significantly associated (p < 0.05). Anti‐microbial susceptibility testing of VRSA isolates exhibited the highest resistance to oxytetracycline, followed by oxacillin and Trimethoprim+sulfamethoxazole. The current study sequences showed more resemblance with reported sequences from Iraq (MN747834) and Egypt (MK095504, MK087830), which belong to vanB gene from S. aureus as compared to sequences from other countries, which belong to vanB gene from the genus Enterococcus. The Genetic Algorithm for Recombination Detection (GARD) found 234 potential breakpoints, translating into a search room of 123,883,305 models with up to 4 breakpoints. The phylogenetic motif profiling method discovered evolutionarily conserved residues across target genes' homologous protein sequences. These residues were discovered to be conserved in drug‐resistant target proteins over the evolutionary process and may play a key role in their function. The current study revealed a molecular prevalence of VRSA in dairy animals, along with molecular analysis of vancomycin resistance in S. aureus by targeting the vanB gene using standard bioinformatics tools. The occurrence of VRSA in animals requires serious attention because this pathogen has zoonotic potential, so it can become a greater risk to consumer health. [ABSTRACT FROM AUTHOR]

Objective: Schistosomiasis is a neglected tropical parasitic disease caused by blood flukes of the genus Schistosoma. Schistosoma japonicum is zoonotic in China, the Philippines, and Indonesia, with bovines acting as major reservoirs of human infection. The primary objective of the trial was to examine the impact of a combination of human mass chemotherapy, snail control through mollusciciding, and SjCTPI bovine vaccination on the rate of human infection. Methods: A 5-year phase IIIa cluster randomized control trial was conducted among 18 schistosomiasis-endemic villages comprising 18,221 residents in Northern Samar, The Philippines. Results: Overall, bovine vaccination resulted in a statistically significant decrease in human infection (relative risk [RR] = 0.75; 95% confidence interval [CI] = 0.69 to 0.82) across all trial follow-ups. The best outcome of the trial was when bovine vaccination was combined with snail mollusciciding. This combination resulted in a 31% reduction (RR = 0.69; 95% CI = 0.61 to 0.78) in human infection. Conclusion: This is the first trial to demonstrate the effectiveness of a bovine vaccine for schistosomiasis in reducing human schistosome infection. The trial is registered with Australian New Zealand Clinical Trials Registry (ACTRN12619001048178).

Simple Summary: Animal welfare and stress response assessment need valid animal-based indicators. Saliva has gained relevance as a non-invasive biological fluid that can be used to assess cortisol levels and different parameters of oxidative stress as potential sensitive indicators for evaluating animal welfare and health. The main objective of this work was to verify whether saliva and eye infrared thermography are accurate and efficient methods that can be used to detect transport-induced stress in calves. Our results show that transport increases salivary cortisol, oxidative status parameters, and eye temperatures. Furthermore, this study proves that the saliva of calves, as well as ocular temperature, suffers significant changes in its composition during the transport process, supporting saliva and infrared thermography as effective non-invasive methodologies to accurately assess cows' stress status. Animal transport is currently a stressful procedure. Therefore, animal-based indicators are needed for reliable and non-invasive welfare assessment. Saliva is a biospecimen with potential validity for the determination of cortisol and oxidative stress, although its use to assess calf welfare during transport has never been tested. Similarly, the applicability and reliability of infrared thermography to assess temperature change during calves' transport have never been evaluated. These objectives were outlined following the known and growing need to identify non-invasive methodologies for stress assessment in bovines. This study was conducted on 20 calves of the Arouquesa autochthone breed, at about nine months of age, during their transport to slaughter. For each animal, saliva samples and thermographic images of the eye were collected at three time points: before transport, after transport, and at slaughter. The saliva was then processed to measure cortisol levels and oxidative stress parameters (reactive oxygen species, thiobarbituric acid reactive substance, carbonyls, and advanced oxidation protein products), and the images were analyzed using FLIR Tools+ software. There was an increase in cortisol concentration and oxidative stress parameters (reactive oxygen species, thiobarbituric acid reactive substance, carbonyls, and advanced oxidation protein products) in saliva after transport. An increase in eye temperature triggered by transport was also observed. The cortisol and eye temperature results at slaughter were returned to values similar to those before transport; however, the values of oxidative stress remained increased (mainly TBARS values). These non-invasive techniques seem to be reliable indicators of stress in bovine transport, and oxidative stress parameters in saliva may be a persistent marker for welfare assessment. [ABSTRACT FROM AUTHOR]

This review aimed to provide a critical and systematic evaluation of the scientific literature about the effect of tree arrangements of the silvopasture system on the thermal environment, behaviour, and performance of cattle. We conducted a search using Web of Science and Google Scholar to identify the key literature of the theme. Peer-reviewed published articles written in English, comparing one or more tree arrangements of SPS to treeless pasture or comparing shaded and sunny areas within the SPS, were selected. The resulting manuscripts (n=191) underwent a four-step PRISMA appraisal process. This process resulted in a final sample of 37 articles, which were used for recording metadata, bibliometric analysis, and assessment of the results, using the software R. Of the 37 articles, 20 studies evaluated dairy cattle (behaviour: 14; performance: 7) and 17 evaluated beef cattle (behaviour: 6; performance: 12). The main behaviours evaluated were grazing (95%) and ruminating (90%), while weight gain (89%) was the main performance variable evaluated. The cooccurrence network analysis highlighted that the studies related the animals' response to improving the thermal environment promoted by silvopasture systems. The main difference among tree arrangements evaluated by the studies was tree density, which ranged from 5 to 800 trees/ha; most studies covered SPS with row of trees (single row, n=16 and multiple rows, n=19). Most studies (n=32) were carried out in Brazil. The evidence of this review showed that regardless of tree arrangement, the silvopasture system improves the thermal environment for cattle, although some effects on behaviour and performance are inconclusive. [ABSTRACT FROM AUTHOR]

Background and Aim: Subclinical mastitis (SCM) caused by erythromycin-resistant Staphylococcus aureus is a significant disease in lactating animals. Therefore, it is crucial to understand the genetic factors contributing to erythromycin resistance in S. aureus. This study aimed to estimate the prevalence of S. aureus in milk from subclinical mastitic cattle and buffaloes and tank milk samples as identified by probe-based real-time polymerase chain reaction (PCR) and the genotypic assessment of macrolide and erythromycin resistance profiles, as well as to analyze the phylogenetic relatedness of our local isolates of S. aureus. Materials and Methods: In total, 285 milk samples were analyzed using the California mastitis test to detect SCM. Milk samples were cultured on different specific Staphylococcus media. The presence of S. aureus was confirmed by Gram staining, the catalase and coagulase tests, the detection of hemolytic activity, DNase agar testing, and biofilm activity in Congo red medium. The genotypic identification of S. aureus (nuc) was performed. The determinants of erythromycin (ermA, ermB, ermC, and ermT) and macrolide resistance (msrA) were screened in all isolates. DNA sequencing of our local isolates of S. aureus was used to analyze their phylogenetic relatedness. Moreover, histopathological examination of tissue specimens of mammary gland was performed. Results: The S. aureus positivity rates were 36.4%, 48.8%, and 63.6% in cattle, buffalo, and bulk tank milk, respectively. Probe-based real-time PCR molecularly confirmed all 62 S. aureus isolates. Thirty-one isolates were subjected to PCR to create profiles of their genotypic erythromycin resistance. ermA, ermB, ermC, and ermT were present in 5 (8%), 26 (41.9%), 18 (29%), and 15 (24.1%) S. aureus isolates, respectively. Moreover, msrA was found in three (4.8%) strains. Eight PCR products were produced using standard PCR for DNA sequencing. Multiple sequence alignment, phylogenetic tree construction, and analysis of nuc in S. aureus revealed a high degree of homology (100%) with S. aureus strains isolated from milk in cases of bovine mastitis in India and Kenya. Histological analysis of udder tissues revealed extensive aggregation of mononuclear inflammatory cells in the interstitial connective tissue, primarily lymphocytes, and macrophages. Conclusion: This study showed a high prevalence of erythromycin resistance in S. aureus isolates. This information is vital for controlling mastitis and the spread of resistance genes between bacterial strains and hosts. Moreover, the probe-based real-time PCR approach is helpful for the rapid screening of S. aureus isolates and the consequent efficient treatment and control of S. aureus mastitis. [ABSTRACT FROM AUTHOR]

The current work aims to analyze the existing situation in Romania compared to other EU countries, regarding bovines herds, given that this species is one of the ones that causes the highest level of greenhouse gases, with a direct effect on the environment and global warming. This is the reason why the subject has become a priority not only at the community level, but also worldwide. Through the Common Agricultural Policy, important funds have been allocated at the level of the European Union, which will continue to be allocated, not only for the reduction of climate change, but also for the adaptation of the states of the world to these existing climate changes at the global level. Given the path in which animal husbandry influences sustainable development, through the study we have followed both the effective changes, but also the obtained productions, with a direct impact on consumption. It was found that both at the community level, as well as at the national level, there was a decrease in bovines herds in in the period 2016-2020, the reasons being primarily of an economic nature, but equally important being the social and environmental ones. The analysis was carried out starting from the data published by the National Institute of Statistics and Eurostat, data that were then processed and analyzed with the help of statistical methods. Based on these, the conclusions were formulated that show us that in Romania the number of bovines herds decreased by 11% in the period 2016-2021. At the European Union level, Romania ranks 10th in terms of bovines herds and 20th in terms of density/100 ha. Although there are advances in terms of applied technologies or more productive breeds, at the global level the objectives pursued by the reduction of greenhouse gas emissions have not been influenced by these advances, which makes the activity of raising bovines to be considered one with effects negative effects on the environment, but also high consumption of energy and water, which in turn have a negative impact on climate change. [ABSTRACT FROM AUTHOR]

Copyright of Compendium of Veterinary Sciences / Compendio de Ciencias Veterinarias is the property of Facultad de Ciencias Veterinarias de la Universidad Nacional de Asuncion and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Revista Brasileira de Reprodução Animal is the property of Revista Brasileira de Reproducao Animal and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Simple Summary: This systematic review addresses animal welfare-related aspects associated with the transport and slaughter of cattle. Before transport, the husbandry system and health status of cattle can have a major impact on animal welfare. At the abattoir, personnel may inflict stress on the animals when moving them to the lairage pens and to the stunning box. Constructional conditions and the resulting environmental effects have a major influence on stress induction as well. Stress can be assessed by both behavioural observations and measurement of physiological parameters. Rapid and effective stunning is an important welfare-related criterion. Some easily verifiable and detectable indicators of unconsciousness, such as immediate collapse after stunning, loss of rhythmic breathing, and loss of the corneal reflex are routinely monitored at the abattoir. Other aspects, such as measuring stress hormones in the blood or using an electrocardiogram during stunning, provide scientific information but are neither practically nor financially achievable in routine procedures. Expertise and training of drivers and abattoir personnel are an important contribution to stress reduction during handling of cattle and, therefore, to animal welfare during transport and slaughter and, finally, to meat quality. Literature related to European transport and slaughter processes were included in this systematic review. The publication period is limited to the past twelve years since the European Animal Welfare Transport Regulation was enacted in 2009. Three different databases were used. The final screening resulted in the inclusion of 19 articles in this review. When handling cattle during transport and slaughter, personnel have an important impact and may inflict stress on the animals. Other factors, such as the group composition and health status prior to transport, can have a strong negative effect on animal welfare. At the abattoir, constructional conditions and the resulting environmental influences can have a negative impact on welfare as well. These include increased noise levels due to the lack of noise dampening and changing light conditions. Stress in cattle can be assessed, e.g., by measuring stress hormones or heart rate. Effective stunning is an important welfare-relevant step in the slaughtering process. Some signs of unconsciousness, such as immediate body collapse or absence of the corneal reflex, can be easily assessed. Expertise and continuous training of all personnel involved are important measures in stress reduction. [ABSTRACT FROM AUTHOR]

This article analyzes the inter-body perception between humans and cattle, which were raised and commercialized as commodity. It takes as an empirical reference a family of puesteros who lives in the Alberdi department, located in the Argentine province of Santiago del Estero. The text contributes to the understanding of the characteristics assumed by the perceptual, gestural and affective links between humans and bovine animals of a socio-technical network of extensive forest breeding. It is argued that this interaction between species results in a particular form of affective, sentient and individuated inter-bodily connection with bovine alterities, and, at the same time, in an asymmetric technical and commercial bond. Thus, in the experience of the cows coexist the duality of being objects-commodities and living/sentient beings, two forms of perception that at first could seem contradictory. [ABSTRACT FROM AUTHOR]

Staphylococcus aureus (S. aureus), the bacteria most frequently associated with mastitis in cattle and buffalo, has a large number of genes connected to antibiotic resistance. The objective of the current research was to determine the erythromycin and macrolide resistance of S. aureus isolated from mastitic milk of cattle and buffalo, particularly resistance-related genes (ermA, ermB, ermC, ermT, and msrA). Therefore, 150 dairy cattle and 135 dairy buffalo bred by small farmers in various governorates of Egypt (Cairo, Giza, Kalyobia, Fayoum, and Kafr El-Sheikh) provided a total of 285 milk samples. Inspection revealed that a total of 34 (22.7%) and 36 (26.7%) milk samples from cattle and buffalo, respectively, had clinical mastitis. With a total recovery of 31 (44.3%) S. aureus isolates. Bacterial isolation and identification of S. aureus verified the isolation and identification of 14 (41.2%) and 17 (47.2%) S. aureus isolates from cattle and buffalo, respectively. Utilizing a TaqMan probe-based real-time PCR method that targets the nuc gene, all S. aureus isolates were verified. In instances of bovine mastitis in India and Kenya, conventional PCR targeting the nuc gene, followed by DNA sequencing and phylogenetic analysis, revealed a high homology (100%) with that of S. aureus strains isolated from milk. For the tested genes, the prevalence of resistant strains was 9.6% (ermA), 64.5% (ermB), 70.9% (ermC), 19.3% (ermT), and 9.6%. (msrA). Therefore, effective control measures should be adopted to stop the spread of drug-resistant S. aureus to humans. [ABSTRACT FROM AUTHOR]

• Schistosomiasis is a neglected tropical parasitic disease caused by the blood fluke Schistosoma. • Praziquantel is the mainstay of treatment and community-based control, but it is failing in some regions. • Schistosomiasis control in Asia is complicated by the zoonotic nature of the disease. • Vaccination of bovines has been proposed as a tool to assist in long-term control. • We report the results of a cluster randomized control trial using a SjCTPI-Hsp70 bovine vaccine. Schistosomiasis is a neglected tropical parasitic disease caused by blood flukes of the genus Schistosoma. Schistosoma japonicum is zoonotic in China, the Philippines, and Indonesia, with bovines acting as major reservoirs of human infection. The primary objective of the trial was to examine the impact of a combination of human mass chemotherapy, snail control through mollusciciding, and SjCTPI bovine vaccination on the rate of human infection. A 5-year phase IIIa cluster randomized control trial was conducted among 18 schistosomiasis-endemic villages comprising 18,221 residents in Northern Samar, The Philippines. Overall, bovine vaccination resulted in a statistically significant decrease in human infection (relative risk [RR] = 0.75; 95% confidence interval [CI] = 0.69 to 0.82) across all trial follow-ups. The best outcome of the trial was when bovine vaccination was combined with snail mollusciciding. This combination resulted in a 31% reduction (RR = 0.69; 95% CI = 0.61 to 0.78) in human infection. This is the first trial to demonstrate the effectiveness of a bovine vaccine for schistosomiasis in reducing human schistosome infection. The trial is registered with Australian New Zealand Clinical Trials Registry (ACTRN12619001048178). [ABSTRACT FROM AUTHOR]

The aim of this study was to isolate and characterize bovine-vaginal probiotics genotypically and phenotypically using in silico and evaluate their in vivo performance in buffaloes with endometritis. For the in vitro isolation and characterization, vaginal swabs were collected from 34 cows and 17 buffaloes, and 709 primary bacterial isolates with probiotic activity were obtained using MRS agar media. Two isolates Lactiplantibacillus plantarum KUGBRC (LPKUGBRC) and Pediococcus pentosaceus GBRCKU (PPGBRCKU) demonstrated optimum in vitro probiotic activities as compared to Lacticaseibacillus rhamnosus GG including, acid production, secretion of fatty acids and exopolysaccharide, cell surface hydrophobicity, self-aggregating and co-aggregating capacity with pathogens, anti-microbial activity and bacteriocin-like compounds against pathogens Escherichia coli and Staphylococcus aureus in cell-free supernatant and absence of hemolytic activity. Their phenotypic capacity was confirmed by analyzing the whole genome sequencing data and identifying genes and pathways associated with probiotic properties. These probiotic isolates have shown no virulence genes were discovered in their genomic study. In vivo study of 92 buffaloes suffering from clinical endometritis with purulent cervico-vaginal mucus (CVM) were randomly allocated 40 × 108 CFU/ml LPKUGBRC and PPGBRCKU and 40 ml Normal saline. The LPKUGBRC reduced the duration between administration of probiotic to induction of healthy estrus significantly. However, no effect was observed on pregnancy rate. These results suggest that LPKUGBRC and PPGBRCKU probiotic bacteria demonstrate probiotic efficiency and adaptability. Further sourced from the same niche as the targeted infection, they offer a distinct advantage in targeting the specific microbial population associated with endometritis. The findings of this study highlight the potential of LPKUGBRC and PPGBRCKU probiotics in treating endometritis and suggest further exploration of their clinical applications.

Abstract Staphylococcus aureus is a common mastitis pathogen in dairy cows, and methicillin-resistant S. aureus (MRSA) has been found in dairy farms all over the world. The study carried out on bovines from three governorates in Egypt, with the goal of determining the prevalence of MRSA in positive milk samples of subclinical mastitis, performing an antibiotic susceptibility test against MRSA isolates and determining the risk factors associated with MRSA. A total of 350 quarter milk samples (n = 200 mixed breed cow; n = 150 water buffalo) were collected and examined for subclinical mastitis using the California mastitis test (CMT) before being exposed to standard microbiological procedures for S. aureus isolation. The disc diffusion method was used to phenotypically analyse the positive S. aureus isolates for MRSA, which was verified by a PCR assay targeting the mecA gene. According to the findings of the study, 41.4% (145/350) milk samples were positive based on CMT, while 35.7% (125/350) of positive samples identified as MRSA based on PCR assay. However, the obtained results revealed non-significant disparity between cattle and buffalo and all predicted risk factors were strongly associated with prevalence of subclinical mastitis. The in-vitro antibiotic susceptibility test revealed that cefoxitin was completely resistant, whereas linezolid, ciprofloxacin, and trimethoprim + sulphamethoxazole were sensitive against the MRSA isolates. The relevance of S. aureus to public health, as well as the development of resistance to antibiotics like methicillin, needs ongoing testing of antimicrobial medications against MRSA isolates.

The present study aimed to evaluate the antimicrobial usage (AMU) pattern in dairy herds of Punjab, India. The on-farm quantification of AMU in adult bovine animals by the manual collection of empty drug containers (“bin method”) along with the records of the treatment was carried out in 38 dairy farms involving 1010 adult bovines for 1 year from July 2020 to June 2021. The farm owners were asked to record the antibiotic treatments as well as to deposit empty antibiotic packaging/vials into the provided bins placed at the farms. A total of 14 different antibiotic agents in 265 commercial antibiotic products were administered to the dairy herds during the study. A total of 179 (67.55%) administered products contained antimicrobials of “critical importance” as per the World Health Organization (WHO). Mastitis (54.72%), followed by the treatment of fever (19.62%), reproductive problems (15.47%), and diarrhea (3.40%) accounted for the majority of drugs administered in the herds during the study period. The most commonly used antibiotics were enrofloxacin (89.47% herds; 21.51% products), followed by ceftriaxone (50% herds; 12.83% products), amoxicillin (50% herds; 12.83% products), oxytetracycline (55.26% herds; 11.70% products), and procaine penicillin (47.37% herds; 12.83% products). The highest quantity of AMU [in terms of antimicrobial drug use rate (ADUR)] was observed for ceftiofur, followed by ceftriaxone, procaine benzyl penicillin ceftizoxime, enrofloxacin, cefoperazone, amoxicillin and ampicillin. A total of 125 (47.17%) products contained “highest priority critically important antimicrobials” (HPCIA) and 54 (20.37%) products contained “high priority critically important antimicrobials”. In terms of overall number of animal daily doses (nADD), the highest priority critically important antimicrobials (HPCIA) of the WHO such as third-generation cephalosporins and quinolones, respectively accounted for 44.64 and 22.35% of the total antibiotic use in the herds. The bin method offers an alternative to monitoring AMU as a more accessible approach for recording the actual consumption of antimicrobials. The present study, to the best of our knowledge, is the first of its kind to provide an overview of the qualitative and quantitative estimate of AMU among adult bovines from India.

In order to address the immediate impact of COVID-19 on dairy farming in the province of Santo Domingo de los Tsáchilas, Ecuador, qualitative longitudinal research was carried out, supported by the structured interview of 86 producers belonging to the Association of Cattlemen of the province. The qualitative variables (health management and impact of COVID- 19) were analyzed through statistical frequencies (absolute and relative), and their distribution was compared through chi-square tests and likelihood ratio, before and during the COVID-19 pandemic. It was found that the variables of antiparasitic product for adult bovines (p ˂ 0.05) and frequency of deworming of young bovines (p ˂ 0.01) were affected by the condition derived from the COVID-19 pandemic. 46% of the producers were infected by COVID-19, and more than 70% of them have had at least one family member and one worker infected. The COVID-19 pandemic forced producers to modify their work plan in the sanitary management of dairy cattle, particularly immunization plans and deworming strategies. Therefore, the resilience shown by the producers was essential for the continuity of the livestock activity. [ABSTRACT FROM AUTHOR]

There is an increasing interest in the genetic quantification of bovine DNA. Analysis of genetic diversity using DNA extracted from the blood is affected by the quality and the quantity of the DNA extracts, which are critical factors that limit the accuracy and sensitivity of molecular studies. The purpose of this paper is to show the quantity and purity of DNA isolated from a number of 24 blood samples from Pinzgau cattle, using two basic techniques, the automatic DNA extraction technique, using Maxwell™ 16 and 16 MDx instruments and the spectrophotometric quantification technique, using Nanodrop ASP-3700 spectrophotometer. The obtained results are considered satisfactory in terms of the purity of the obtained DNA extracts, 13 samples having the DNA isolate contaminated with proteins based on the absorbance ratio A260/A280 lower than 1.7, the solution in this situation being the repetition of the protein precipitation process. [ABSTRACT FROM AUTHOR]

Copyright of ESPOCH Congresses: The Ecuadorian Journal of S.T.E.A.M. is the property of Knowledge E DMCC and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

This paper describes an outbreak of nephrosis in cattle associated with the consumption of Ludwigia peploides in Tucuman province, northwestern Argentina. Affected cows exhibited submandibular and chest edema, lethargy and ataxia, and eventually succumbed to these conditions. These animals were grazing in a floodable area severely invaded by this plant. The disease affected 7 out of a herd of 67 cows. Horses and goats grazing in the same location were not affected. The main gross and histological lesion corresponded to a severe nephrosis. The disease is similar to the poisoning by Ludwigia peruviana reported in Colombia. [Display omitted] • Consumption of Ludwigia peploides causes nephropathy in cattle. • To our knowledge, this is the first report of Ludwigia peploides in Argentina and South America. • Intoxication by this species occurs in floodplain fields during periods of food scarcity. • According to bibliographic records, intoxication occurs after prolonged consumption. [ABSTRACT FROM AUTHOR]

Background: The Gyr breed is widely used in Colombian low tropic dairy production systems. During the last 10 years, the Asociación Colombiana de Criadores de Ganado Cebú† - ASOCEBU, has been leading a dairy milk control program which led to the creation of a dataset that permits to carry out the first analysis of milk yield in Gyr cattle in the country using records from several herds. Objectives: To study milk production dynamics of Gyr cattle in the Colombian low tropic through the estimation of lactation curves and four derived production parameters: total milk yield between 5 and 305 days (TMY305), peak milk yield (PMY), days at peak (DP) and persistency (P). Methods: 13,798 daily milk yield records from 1,510 cows performing in 103 herds were used; the total number of lactations was 2,480. Four models were considered: Wood, Wiltmink, Papajcsik & Bordero, and a second-degree polynomial. Mean square error, mean absolute error, mean square error of prediction, Akaike and Bayesian information criteria were used to select the model better describing each lactation using the majority rule, that is, the model selected by most criteria was the chosen one. The shape of each fitted lactation curve was checked using basic results from calculus which permitted the classification of the estimated curves into two groups: typical and atypical; only typical functions were used to compute the four aforementioned production parameters. Results: The second-order polynomial was the model most frequently selected, while the Papajcsik & Bordero model had the lowest frequency. Average TMY305, PMY, DP and P were 3,489.86 kg, 17.28 kg, 57.17 days, and 0.83, respectively, with coefficients of variation: 0.27, 0.21, 0.41, and 0.16. Conclusions: This study permitted to identify individuals with outstanding phenotypic performance. To the best of our knowledge, this is the first study of this kind involving thousands of lactations from Gyr cows performing in several regions of Colombian low tropic.

Objectives: Aimless use of anti-microbials for the treatment of uterine diseases has driven the rise of safe strains. Subsequently, within the current consider, the viability of post calving tonic (PCT) was assessed in vivo for the treatment of post calving complications in dairy cattle. Materials and Methods: The placentas of 10 chosen post-calved dairy animals with a history of postpartum complications primarily held placentas were drenched with PCT 250 ml twice a day for 2 successive days. The evaluation parameters, viz., time taken for removal of lochia, placenta, involution of the uterus and estrus come back time, body condition score, and milk yield have been analyzed to assess the efficacy of PCT in post calved dairy bovines. Results: The results depicted that following the administration of PCT, the mean time taken for expulsion of uterine discharge (lochia), placenta, time taken for the involution of the uterus, and estrus come back time was 86 and 10 h, 30 and 36 days, respectively. Additionally, the administration of PCT 250 ml twice daily for 2 consecutive days to the post-calved dairy cows caused a significant increase (p < 0.05) in milk yield. Conclusion: It was apparent that PCT encourages the unconstrained removal of held placenta through its ecbolic movement and advances uterine discharge exercises. In addition, PCT supplementation caused augmentation of milk yield in post calved dairy cows. [J Adv Vet Anim Res 2021; 8(4.000): 650-655]

Gastrointestinal parasites in production cattle are one of the most important health problems worldwide. These nematodes cause gastroenteric problems that are clinically characterized by diarrhoea, weakness, bleeding and dehydration. Within the gastrointestinal parasites caused by nematodes in cattle, the following families stand out: Trichuridae, Trichostrongylidae, Ancylostomidae, Ascaridae and Strongyloididae. The purpose of this research was to determine the efficacy of the different qualitative and quantitative coproparasitological techniques for the detection of gastrointestinal nematodes in cattle. For this study, 250 samples of beef breed cows were used, divided into different groups, for the application of qualitative and quantitative coproparasitological techniques, which were specifically chosen taking into account inclusion and exclusion criteria for each animal. The results delivered by this study indicated that the highest percentage in the identification of parasitic infective states was through qualitative coproparasitological techniques, focused mainly on zinc and magnesium sulfate flotation techniques with 50%. [ABSTRACT FROM AUTHOR]

Coxiella burnetii causes query (Q) fever in bovines. Its role has never been elucidated in bovines from Pakistan. The current study was designed to determine the incidence of coxiellosis in bovines, evaluate the association of various risk factors in the occurrence of Coxiella burnetii, determine phylogeny and genetic variability of various isolates identified during the study, and report hematology of the affected bovines. The incidence of coxiellosis was estimated as 32.12% and 12.5% in cows and buffaloes, respectively. The association of selected clinical biomarkers was also ascertained and all of these were found significantly (p<0.05) associated with the incidence of coxiellosis. The results of animal-related risk factors indicated that with increasing age and parity number, poor BCS, or a history of disease, the incidence of coxiellosis became significantly (p<0.05) higher. Various farm-related risk factors were also found significantly (p<0.05) associated with Coxiellosis. Moreover, phylogeny of isolated bacteria showed genetic variability (47%-99%). Lastly, the hematology profile of Coxiella burnetii positive buffaloes showed significant (p<0.05) lymphocytopenia (45.95 ± 13.4), neutrophilia (32.80 ± 11.75), leukocytosis (11.25 ± 3.62), eosinopenia (4.95 ± 2.14), thrombocytopenia (232.50 ± 211.74), and decreased hemoglobin (7.005 ± 0.90) and PCV levels (21.29 ± 2.28). Whereas in affected cows only low hemoglobin level (7.25 ± 1.18), decreased PCV (22.29 ± 3.34), eosinopenia (9.6 ± 5.49), and thrombocytopenia (365.25 ± 227.32) were statistically significant (p<0.05). As far as we know, this study provides the first molecular evidence of coxiellosis in bovines from Pakistan. [ABSTRACT FROM AUTHOR]