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10 results on '"Bouyoucef, Mouloud"'

4. Enhanced chondrogenesis of bone marrow-derived stem cells by using a combinatory cell therapy strategy with BMP-2/TGF-β1, hypoxia, and COL1A1/HtrA1 siRNAs

Author

Legendre, Florence, Ollitrault, David, Gómez-Leduc, Tangni, Bouyoucef, Mouloud, Hervieu, Magalie, Gruchy, Nicolas, Mallein-Gerin, Frédéric, Leclercq, Sylvain, Demoor, Magali, Galéra, Philippe, Biologie, génétique et thérapies ostéoarticulaires et respiratoires (BIOTARGEN), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), Service de Génétique [CHU Caen], CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Université de Caen Normandie (UNICAEN), Normandie Université (NU), Institut de biologie et chimie des protéines [Lyon] (IBCP), Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)

Subjects
Male, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, Science, Cell- and Tissue-Based Therapy, Bone Morphogenetic Protein 2, Mice, Nude, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Mesenchymal Stem Cell Transplantation, Collagen Type I, Article, Transforming Growth Factor beta1, Mice, Chondrocytes, Bone Marrow, Osteoarthritis, chondrogenesis, Animals, Humans, RNA, Small Interfering, Cells, Cultured, Aged, Aged, 80 and over, Tissue Engineering, hypoxia, Cell Differentiation, Mesenchymal Stem Cells, High-Temperature Requirement A Serine Peptidase 1, Middle Aged, BM-MSC, Collagen Type I, alpha 1 Chain, siRNA, Medicine, Female
Abstract

International audience; Mesenchymal stem cells (MSCs) hold promise for cartilage engineering. Here, we aimed to determine the best culture conditions to induce chondrogenesis of MSCs isolated from bone marrow (BM) of aged osteoarthritis (OA) patients. We showed that these BM-MSCs proliferate slowly, are not uniformly positive for stem cell markers, and maintain their multilineage potential throughout multiple passages. The chondrogenic lineage of BM-MSCs was induced in collagen scaffolds, under normoxia or hypoxia, by BMP-2 and/or TGF-β1. The best chondrogenic induction, with the least hypertrophic induction, was obtained with the combination of BMP-2 and TGF-β1 under hypoxia. Differentiated BM-MSCs were then transfected with siRNAs targeting two markers overexpressed in OA chondrocytes, type I collagen and/or HtrA1 protease. siRNAs significantly decreased mRNA and protein levels of type I collagen and HtrA1, resulting in a more typical chondrocyte phenotype, but with frequent calcification of the subcutaneously implanted constructs in a nude mouse model. Our 3D culture model with BMP-2/TGF-β1 and COL1A1/HtrA1 siRNAs was not effective in producing a cartilage-like matrix in vivo. Further optimization is needed to stabilize the chondrocyte phenotype of differentiated BM-MSCs. Nevertheless, this study offers the opportunity to develop a combinatory cellular therapy strategy for cartilage tissue engineering.

Published
2017
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5. Enhanced chondrogenesis of bone marrow-derived stem cells by using a combinatory cell therapy strategy with BMP-2/TGF-β1, hypoxia, and COL1A1/HtrA1 siRNAs

Author

Branly, Thomas, Contentin, Romain, Desancé, Mélanie, Jacquel, Thibaud, Bertoni, Lelia, JACQUET, Sandrine, Denoix, Jean-Marie, Audigié, Fabrice, Legendre, Florence, Ollitrault, David, Gómez-Leduc, Tangni, Bouyoucef, Mouloud, Hervieu, Magalie, Gruchy, Nicolas, Mallein-Gerin, Frédéric, Leclercq, Sylvain, Demoor, Magali, Galéra, Philippe, Microenvironnement cellulaire et pathologie (MILPAT), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), Biomécanique et Pathologie Locomotrice du Cheval (BPLC), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), Institut universitaire de formation des maîtres - Champagne-Ardenne (IUFM Champagne-Ardenne), Université de Reims Champagne-Ardenne (URCA), Unité de Recherche sur les Maladies Cardiovasculaires, du Métabolisme et de la Nutrition = Institute of cardiometabolism and nutrition (ICAN), Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [APHP]-Sorbonne Université (SU), Normandie Université (NU), Matrice extracellulaire normale et pathologique, Laboratoire de cytogénétique prénatale [CHU Caen], Normandie Université (NU)-Normandie Université (NU)-CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN), Institut de biologie et chimie des protéines [Lyon] (IBCP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), EDF R&D (EDF R&D), EDF (EDF), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], and Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)

Subjects
[SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2017

6. Hypoxia inducible factor 1 alpha down-regulates type i collagen through Sp3 transcription factor in human chondrocytes

Author

Duval, Elise, Bouyoucef, Mouloud, Leclercq, Sylvain, Baugé, Catherine, Boumediene, Karim, Normandie Université (NU), Microenvironnement et Pathologies du Cartilage (BioConnecT), Microenvironnement cellulaire et pathologie (MILPAT), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), and Federation Hospitalo-Universitaire SURFACE

Subjects
musculoskeletal diseases, hypoxia, [SDV]Life Sciences [q-bio], collagen type I, chondrocytes, Gene Expression Regulation, Developmental, Cell Differentiation, Genetic Therapy, Hypoxia-Inducible Factor 1, alpha Subunit, hypoxia-inducible factor 1, Collagen Type I, alpha 1 Chain, Sp3 Transcription Factor, gene expression, Humans, Promoter Regions, Genetic, cartilage, Cartilage Diseases, Sp transcription factors
Abstract

Cartilage engineering is one challenging issue in regenerative medicine. Low oxygen tension or hypoxia inducible factor-1 (HIF-1α) gene therapy are promising strategies in the field of cartilage repair. Previously, we showed that hypoxia and its mediator HIF-1 regulate matrix genes expression (collagens and aggrecan). Here, we investigated the molecular mechanism involved in the regulation of type I collagen (COL1A1) by HIF-1 in human articular chondrocytes. We show that HIF-1α reduces COL1A1 transcription, through a distal promoter (-2300 to -1816 bp upstream transcription initiation site), containing two GC boxes that bind Sp transcription factors (Sp1/Sp3). Sp1 acts as a positive regulator but is not induced by HIF-1. COL1A1 inhibition caused by HIF-1 implies only Sp3, which accumulates and competes Sp1 binding on COL1A1 promoter. Additionally, Sp3 ectopic expression inhibits COL1A1, while Sp3 knockdown counteracts the downregulation of COL1A1 induced by HIF-1. In conclusion, we established a new regulatory model of COL1A1 regulation by HIF-1, and bring out its relationship with Sp3 transcription factor. In a fundamental level, these findings give insights in the mechanisms controlling COL1A1 gene expression. This may be helpful to improve strategies to impair type I collagen expression during chondrocyte differentiation for cartilage engineering. © 2016 IUBMB Life, 68(9):756-763, 2016.

Published
2016
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9. Shell Extracts from the Marine Bivalve Pecten maximus Regulate the Synthesis of Extracellular Matrix in Primary Cultured Human Skin Fibroblasts

Author

Latire, Thomas, primary, Legendre, Florence, additional, Bigot, Nicolas, additional, Carduner, Ludovic, additional, Kellouche, Sabrina, additional, Bouyoucef, Mouloud, additional, Carreiras, Franck, additional, Marin, Frédéric, additional, Lebel, Jean-Marc, additional, Galéra, Philippe, additional, and Serpentini, Antoine, additional

Published
2014
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10. Shell Extracts from the Marine Bivalve Pecten maximus Regulate the Synthesis of Extracellular Matrix in Primary Cultured Human Skin Fibroblasts.

Author

Latire, Thomas, Legendre, Florence, Bigot, Nicolas, Carduner, Ludovic, Kellouche, Sabrina, Bouyoucef, Mouloud, Carreiras, Franck, Marin, Frédéric, Lebel, Jean-Marc, Galéra, Philippe, and Serpentini, Antoine

Subjects
PECTEN maximus, EXTRACELLULAR matrix, SKIN physiology, FIBROBLASTS, CYTOCHEMISTRY, CELLULAR signal transduction
Abstract

Mollusc shells are composed of more than 95% calcium carbonate and less than 5% of an organic matrix consisting mostly of proteins, glycoproteins and polysaccharides. Previous studies have elucidated the biological activities of the shell matrices from bivalve molluscs on skin, especially on the expression of the extracellular matrix components of fibroblasts. In this work, we have investigated the potential biological activities of shell matrix components extracted from the shell of the scallop Pecten maximus on human fibroblasts in primary culture. Firstly, we demonstrated that shell matrix components had different effects on general cellular activities. Secondly, we have shown that the shell matrix components stimulate the synthesis of type I and III collagens, as well as that of sulphated GAGs. The increased expression of type I collagen is likely mediated by the recruitment of transactivating factors (Sp1, Sp3 and human c-Krox) in the −112/−61 bp COL1A1 promoter region. Finally, contrarily to what was obtained in previous works, we demonstrated that the scallop shell extracts have only a small effect on cell migration during in vitro wound tests and have no effect on cell proliferation. Thus, our research emphasizes the potential use of shell matrix of Pecten maximus for dermo-cosmetic applications. [ABSTRACT FROM AUTHOR]

Published
2014
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