5 results on '"Bouwmeester, Manon C."'
Search Results
2. Bioprinting of Human Liver-derived Epithelial Organoids for Toxicity Studies
- Author
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Bouwmeester, Manon C, Bernal, Paulina Nunez, Oosterhoff, Loes A, van Wolferen, Monique, Lehmann, Vivian, Vermaas, Monique, Buchholz, Maj-Britt, Peiffer, Quentin, Malda, Jos, van der Laan, Luc J W, Kramer, Nynke I, Schneeberger, Kerstin, Levato, Riccardo, Spee, Bart, Interne geneeskunde GD, dCSCA RMSC-1, Equine Musculoskeletal Biology, dES RMSC, dIRAS RA-1, CS_STEM, Interne geneeskunde GD, dCSCA RMSC-1, Equine Musculoskeletal Biology, dES RMSC, dIRAS RA-1, CS_STEM, and Surgery
- Subjects
Polymers and Plastics ,Cell ,Cell Culture Techniques ,in vitro modeling ,Bioengineering ,02 engineering and technology ,Toxicology ,drug induced liver injury ,Biomaterials ,03 medical and health sciences ,SDG 3 - Good Health and Well-being ,medicine ,Organoid ,Materials Chemistry ,Humans ,Viability assay ,Cells, Cultured ,Toxicologie ,organoids ,030304 developmental biology ,Liver injury ,0303 health sciences ,Human liver ,Tissue Engineering ,Chemistry ,Bioprinting ,021001 nanoscience & nanotechnology ,medicine.disease ,In vitro ,Cell biology ,medicine.anatomical_structure ,Liver ,Toxicity ,Printing, Three-Dimensional ,extrusion-based bioprinting ,0210 nano-technology ,Biofabrication ,Biotechnology - Abstract
There is a need for long-lived hepatic in vitro models to better predict drug induced liver injury (DILI). Human liver-derived epithelial organoids are a promising cell source for advanced in vitro models. Here, organoid technology is combined with biofabrication techniques, which holds great potential for the design of in vitro models with complex and customizable architectures. Here, porous constructs with human hepatocyte-like cells derived from organoids are generated using extrusion-based printing technology. Cell viability of bioprinted organoids remains stable for up to ten days (88–107% cell viability compared to the day of printing). The expression of hepatic markers, transporters, and phase I enzymes increased compared to undifferentiated controls, and is comparable to non-printed controls. Exposure to acetaminophen, a well-known hepatotoxic compound, decreases cell viability of bioprinted liver organoids to 21–51% (p < 0.05) compared to the start of exposure, and elevated levels of damage marker miR-122 are observed in the culture medium, indicating the potential use of the bioprinted constructs for toxicity testing. In conclusion, human liver-derived epithelial organoids can be combined with a biofabrication approach, thereby paving the way to create perfusable, complex constructs which can be used as toxicology- and disease-models.
- Published
- 2021
3. Zebrafish embryos as a screen for DNA methylation modifications after compound exposure
- Author
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Bouwmeester, Manon C., Ruiter, Sander, Lommelaars, Tobias, Sippel, Josefine, Hodemaekers, Hennie M., van den Brandhof, Evert-Jan, Pennings, Jeroen L.A., Kamstra, Jorke H., Jelinek, Jaroslav, Issa, Jean-Pierre J., Legler, Juliette, and van der Ven, Leo T.M.
- Published
- 2016
- Full Text
- View/download PDF
4. Programmed Effects in Neurobehavior and Antioxidative Physiology in Zebrafish Embryonically Exposed to Cadmium: Observations and Hypothesized Adverse Outcome Pathway Framework.
- Author
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Ruiter, Sander, Sippel, Josefine, Bouwmeester, Manon C., Lommelaars, Tobias, Beekhof, Piet, Hodemaekers, Hennie M., Bakker, Frank, van den Brandhof, Evert-Jan, Pennings, Jeroen L. A., and van der Ven, Leo T. M.
- Subjects
NON-communicable diseases ,PHYSIOLOGICAL effects of cadmium ,ZEBRA danio ,PHYSIOLOGICAL effects of antioxidants ,ANTIOXIDANTS & health ,PHYSIOLOGY - Abstract
Non-communicable diseases (NCDs) are a major cause of premature mortality. Recent studies show that predispositions for NCDs may arise from early-life exposure to low concentrations of environmental contaminants. This developmental origins of health and disease (DOHaD) paradigm suggests that programming of an embryo can be disrupted, changing the homeostatic set point of biological functions. Epigenetic alterations are a possible underlying mechanism. Here, we investigated the DOHaD paradigm by exposing zebrafish to subtoxic concentrations of the ubiquitous contaminant cadmium during embryogenesis, followed by growth under normal conditions. Prolonged behavioral responses to physical stress and altered antioxidative physiology were observed approximately ten weeks after termination of embryonal exposure, at concentrations that were 50-3200-fold below the direct embryotoxic concentration, and interpreted as altered developmental programming. Literature was explored for possible mechanistic pathways that link embryonic subtoxic cadmium to the observed apical phenotypes, more specifically, the probability of molecular mechanisms induced by cadmium exposure leading to altered DNA methylation and subsequently to the observed apical phenotypes. This was done using the adverse outcome pathway model framework, and assessing key event relationship plausibility by tailored Bradford-Hill analysis. Thus, cadmium interaction with thiols appeared to be the major contributor to late-life effects. Cadmium-thiol interactions may lead to depletion of the methyl donor S-adenosyl-methionine, resulting in methylome alterations, and may, additionally, result in oxidative stress, which may lead to DNA oxidation, and subsequently altered DNA methyltransferase activity. In this way, DNA methylation may be affected at a critical developmental stage, causing the observed apical phenotypes. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
5. Bioprinting of Human Liver-Derived Epithelial Organoids for Toxicity Studies.
- Author
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Bouwmeester MC, Bernal PN, Oosterhoff LA, van Wolferen ME, Lehmann V, Vermaas M, Buchholz MB, Peiffer QC, Malda J, van der Laan LJW, Kramer NI, Schneeberger K, Levato R, and Spee B
- Subjects
- Cell Culture Techniques, Cells, Cultured, Humans, Bioprinting, Liver cytology, Liver metabolism, Organoids cytology, Organoids metabolism, Printing, Three-Dimensional, Tissue Engineering
- Abstract
There is a need for long-lived hepatic in vitro models to better predict drug induced liver injury (DILI). Human liver-derived epithelial organoids are a promising cell source for advanced in vitro models. Here, organoid technology is combined with biofabrication techniques, which holds great potential for the design of in vitro models with complex and customizable architectures. Here, porous constructs with human hepatocyte-like cells derived from organoids are generated using extrusion-based printing technology. Cell viability of bioprinted organoids remains stable for up to ten days (88-107% cell viability compared to the day of printing). The expression of hepatic markers, transporters, and phase I enzymes increased compared to undifferentiated controls, and is comparable to non-printed controls. Exposure to acetaminophen, a well-known hepatotoxic compound, decreases cell viability of bioprinted liver organoids to 21-51% (p < 0.05) compared to the start of exposure, and elevated levels of damage marker miR-122 are observed in the culture medium, indicating the potential use of the bioprinted constructs for toxicity testing. In conclusion, human liver-derived epithelial organoids can be combined with a biofabrication approach, thereby paving the way to create perfusable, complex constructs which can be used as toxicology- and disease-models., (© 2021 The Authors. Macromolecular Bioscience published by Wiley-VCH GmbH.)
- Published
- 2021
- Full Text
- View/download PDF
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