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7. Cahier thématique : La mer et le littoral de Provence-Alpes-Côte d’Azur face au changement climatique

Author

Babène, A, Bidet, Y, Boudouresque, F, Claeys, Cécilia, David, R, Dhenain, S, Doze, E, Feral, P, Francour, Patrice, Gattuso, Jean-Pierre, Gianni, F, Giuliano, J, Hatt, E, Hilmi, Nathalie, Hizmaj, E, Irisson, Jean-Olivier, Lagneau, C, Lambert, L, Lemee, Rodolphe, Lepetit, A, Mangialajo, Luisa, Marchesseaux, G, Marçot, N, Michalak, S, Mousseau, Laure, Rey, V, Ruitton, S, Rossello, P, Sabatier, F, Safa, A, Sous, D, Stépanian, A, Taupier-Letage, Isabelle, Thibault-Botha, D, Thibaut, M, Thibaut, Thierry, Touboul, J, LABORATOIRE DE CANCEROLOGIE INSERM EMI 0359, Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire Population-Environnement-Développement (LPED), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU), Centre Mémoire de Ressources et de Recherche [Nice] (CMRR Nice), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre Hospitalier Universitaire de Nice (CHU Nice)-Université Côte d'Azur (UCA), Cognition Behaviour Technology (CobTek), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre Hospitalier Universitaire de Nice (CHU Nice)-Institut Claude Pompidou [Nice] (ICP - Nice)-Université Côte d'Azur (UCA), Ecosystèmes Côtiers Marins et Réponses aux Stress (ECOMERS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA), Institut du Développement Durable et des Relations Internationales (IDDRI), Institut d'Études Politiques [IEP] - Paris, Centre Scientifique de Monaco (CSM), Environment Laboratories (IAEA), International Atomic Energy Agency [Vienna] (IAEA), Laboratoire d'océanographie de Villefranche (LOV), Observatoire océanologique de Villefranche-sur-mer (OOVM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de la Mer de Villefranche (IMEV), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche en Génie Civil et Mécanique (GeM), Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-École Centrale de Nantes (ECN)-Centre National de la Recherche Scientifique (CNRS), Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research (C2VN), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre d'Investigation Clinique [Hôpital de la Conception - APHM] (CIC), Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hôpital de la Conception [CHU - APHM] (LA CONCEPTION), Institut méditerranéen d'océanologie (MIO), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS), GREC-PACA, and Les cahiers du GREC-PACA, Association pour l’innovation et la recherche au service du climat (AIR)

Subjects
[SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology
Abstract

International audience

Published
2017

8. Cahier thématique : La mer et le littoral de Provence-Alpes-Côte d’Azur face au changement climatique

Author

Babène, A., Bidet, Y., Boudouresque, F., Cécilia Claeys, Romain DAVID, Dhenain, S., Doze, E., Feral, P., Patrice Francour, Jean-Pierre Gattuso, Gianni, F., Giuliano, J., Hatt, E., Nathalie Hilmi, Hizmaj, E., Jean-Olivier Irisson, Lagneau, C., Lambert, L., Rodolphe Lemee, Lepetit, A., Luisa Mangialajo, Marchesseaux, G., Marçot, N., Michalak, S., Laure Mousseau, Rey, V., Ruitton, S., Rossello, P., Sabatier, F., Safa, A., Sous, D., Stépanian, A., Isabelle Taupier-Letage, Thibault-Botha, D., Thibaut, M., Thierry Thibaut, Touboul, J., LABORATOIRE DE CANCEROLOGIE INSERM EMI 0359, Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire Population-Environnement-Développement (LPED), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU), Centre Mémoire de Ressources et de Recherche [Nice] (CMRR Nice), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre Hospitalier Universitaire de Nice (CHU Nice)-Université Côte d'Azur (UCA), Cognition Behaviour Technology (CobTek), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre Hospitalier Universitaire de Nice (CHU Nice)-Institut Claude Pompidou [Nice] (ICP - Nice)-Université Côte d'Azur (UCA), Ecosystèmes Côtiers Marins et Réponses aux Stress (ECOMERS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA), Institut du Développement Durable et des Relations Internationales (IDDRI), Institut d'Études Politiques [IEP] - Paris, Centre Scientifique de Monaco (CSM), Environment Laboratories (IAEA), International Atomic Energy Agency [Vienna] (IAEA), Laboratoire d'océanographie de Villefranche (LOV), Observatoire océanologique de Villefranche-sur-mer (OOVM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche en Génie Civil et Mécanique (GeM), Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-École Centrale de Nantes (ECN)-Centre National de la Recherche Scientifique (CNRS), Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research (C2VN), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre d'Investigation Clinique [Hôpital de la Conception - APHM] (CIC), Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hôpital de la Conception [CHU - APHM] (LA CONCEPTION), Institut méditerranéen d'océanologie (MIO), and Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology
Abstract

International audience

Published
2017

9. Identification of secondary structure in the 5'-untranslated region of the human adrenomedullin mRNA with implications for the regulation of mRNA translation

Author

L'Houcine Ouafik, Olivier Chinot, Fabienne Brenet, C. Delfino, Boudouresque F, Pierre-Marie Martin, Nadège Dussault, Ouafik, L'Houcine, Rôle et mécanismes d'action de l'adrenomedulline dans la croissance tumorale : Application au modèle des gliomes, Université de la Méditerranée - Aix-Marseille 2-IFR11-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Recherches en Oncologie biologique et Oncopharmacologie (CRO2), Institut National de la Santé et de la Recherche Médicale (INSERM)- Hôpital de la Timone [CHU - APHM] (TIMONE)-Aix Marseille Université (AMU), and Aix Marseille Université (AMU)- Hôpital de la Timone [CHU - APHM] (TIMONE)-Institut National de la Santé et de la Recherche Médicale (INSERM)

Subjects
Untranslated region, Cancer Research, DNA, Complementary, Five prime untranslated region, Molecular Sequence Data, RNA-binding protein, [SDV.CAN]Life Sciences [q-bio]/Cancer, Biology, MESH: Base Sequence, Adrenomedullin, [SDV.CAN] Life Sciences [q-bio]/Cancer, Genes, Reporter, Genetics, Humans, Luciferase, RNA, Messenger, Molecular Biology, Cells, Cultured, MESH: RNA, Messenger, Messenger RNA, MESH: Humans, MESH: Molecular Sequence Data, Base Sequence, MESH: Peptides, MESH: Genes, Reporter, RNA-Binding Proteins, RNA, Translation (biology), MESH: DNA, Complementary, Stem-loop, Molecular biology, MESH: Adrenomedullin, MESH: Sequence Alignm, MESH: RNA-Binding Proteins, MESH: Nucleic Acid Conformation, Protein Biosynthesis, MESH: Protein Biosynthesis, Nucleic Acid Conformation, MESH: 5' Untranslated Regions, 5' Untranslated Regions, Peptides, Sequence Alignment, MESH: Cells, Cultured
Abstract

Adrenomedullin (AM) is a multifunctional regulatory peptide with important angiogenic and mitogenic properties. Here we identify a region of stable secondary structure in the 5'-untranslated region (5' UTR) of human AM mRNA. Reverse transcriptase-polymerase chain reaction of the 5' UTR consistently resulted, in addition to the product with the expected size of 155 base pair (bp), in a second product with an approximately 65-bp deletion from the central region of the 5' UTR, suggesting the presence of a secondary structure. The presence of a stem-loop structure was confirmed by probing the 5' UTR with RNases with selectivity for single- or double-stranded RNA. We investigated the role of this stem-loop structure in expression of luciferase reporter gene in cultured cell lines. Reporter assays using a chimeric mRNA that combined luciferase and the 5' UTR of AM mRNA demonstrated a dramatic decrease of the reporter activity owing to a decreased translation, whereas the deletion of the stem-loop structure localized between nt +31 and +95 from the cap site led to the recovery of activity. Gel migration shift assays using cytosolic extracts from mammalian cell lines demonstrate a specific binding of a cytosolic protein to riboprobes containing the 5' UTR of AM but not to riboprobes either corresponding to other areas of the message or containing the 5' UTR but lacking the region of secondary structure. Although we conclude that the 5' UTR of the human AM mRNA can modulate the translation of AM mRNA in vivo, and that the predicted stem-loop structure is necessary for this inhibition, the functional consequences of the cis element-binding activity remain to be determined.

Published
2006

12. Expression of adrenomedullin and peptide amidation activity in human prostate cancer and in human prostate cancer cell lines

Author

Rocchi, P., Boudouresque, F., Zamora, A. J., Muracciole, X., Lechevallier, E., Martin, P. -M, L’Houcine Ouafik, LABORATOIRE DE CANCEROLOGIE EXPERIMENTALE EA2671, Université de la Méditerranée - Aix-Marseille 2, Laboratoire de Transfert d'Oncologie Biologique [Hôpital Nord - APHM], Hôpital Nord [CHU - APHM]-Assistance Publique - Hôpitaux de Marseille (APHM)-Aix Marseille Université (AMU), Interactions cellulaires neuroendocriniennes (ICN), Université de la Méditerranée - Aix-Marseille 2-Centre National de la Recherche Scientifique (CNRS), Service de Radiothérapie, APHM, Hôpital de la Timone, Marseille, Hôpital de la Timone [CHU - APHM] (TIMONE), SERVICE D'UROLOGIE, Hôpital Salvator [CHU - APHM] (Hôpitaux Sud), Ouafik, L'Houcine, and Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)- Hôpital Nord [CHU - APHM]

Subjects
Male, MESH: Neoplasm Proteins, Neoplasms, Hormone-Dependent, Transplantation, Heterologous, Prostatic Hyperplasia, Mice, Nude, [SDV.CAN]Life Sciences [q-bio]/Cancer, Adenocarcinoma, urologic and male genital diseases, MESH: Multienzyme Complexes, MESH: Phosphopyruvate Hydratase, Mixed Function Oxygenases, Adrenomedullin, Mice, MESH: Prostatic Hyperplasia, MESH: In Situ Hybridization, [SDV.CAN] Life Sciences [q-bio]/Cancer, Multienzyme Complexes, MESH: Reverse Transcriptase Polymerase Chain Reaction, Tumor Cells, Cultured, MESH: Mice, Nude, Animals, Humans, MESH: Animals, RNA, Messenger, MESH: Tumor Cells, Cultured, MESH: Neoplasms, Hormone-Dependent, MESH: Transplantation, Heterologous, MESH: Mice, In Situ Hybridization, MESH: RNA, Messenger, MESH: Humans, Reverse Transcriptase Polymerase Chain Reaction, MESH: Peptides, MESH: Adenocarcinoma, Prostatic Neoplasms, MESH: Immunohistochemistry, MESH: Mixed Function Oxygenases, Immunohistochemistry, MESH: Adrenomedullin, MESH: Male, Neoplasm Proteins, Phosphopyruvate Hydratase, MESH: Prostatic Neoplasms, MESH: Cell Division, Peptides, Cell Division, Neoplasm Transplantation, MESH: Neoplasm Transplantation
Abstract

International audience; After therapeutic hormone deprivation, prostate cancer (CaP) cells often develop androgen-independent growth through not-well-defined mechanisms. The presence of neuroendocrine (NE) cells is often greater in prostate carcinoma than in normal prostate, and the frequency of NE cells correlates with tumor malignancy, loss of androgen sensitivity, increase of autocrine-paracrine activity, and poor prognosis. In some CaPs, neuropeptides have been previously implicated as growth factors. Peptidylglycine alpha-amidating monooxygenase (PAM) is the enzyme producing alpha-amidated bioactive peptides from their inactive glycine-extended precursors. In the present work, we demonstrate that androgen-independent PC-3 and DU145 cell lines, derived from human CaP, express PAM in vitro and in xenografts implanted in athymic nude mice, indicating that they are able to produce alpha-amidated peptides. Contrarily, barely detectable levels of PAM were found in the androgen-sensitive LNCaP cell line. We also show that whereas PC-3 and DU145 cells produce and secrete adrenomedullin (AM), a multifunctional amidated peptide, no expression was found in LNCaP cells. We further demonstrate that AM acts as a growth factor for DU145 cells, which suggests the existence of an autocrine loop mechanism that could potentially drive neoplastic growth. PAM mRNA levels were found to be 3-fold higher in prostate adenocarcinomas compared with that of human benign prostate hyperplasia (BPH) as demonstrated by real-time quantitative reverse transcription-PCR. The analysis of AM message expression in BPH and CaP (Gleason's score, 6-9) shows a clear distinction between benign and CaP. The expression was detected only in adenocarcinomas tissues with a marked increase in samples with a high Gleason's score. Immunocytochemically, AM was localized in the carcinomatous epithelial compartment. NE phenotype, assessed after the immunocytochemical localization of neuron-specific enolase (NSE), was found in both the epithelial and the stromal compartments of cancers; in BPH, only some spare basal cells were NSE-labeled. Cancer progression could be accelerated by peptides secreted by a population of cells capable of inducing androgen-independent tumoral growth via autocrine-paracrine mechanisms.

Published
2001

21. Excitatory amino acids and adenopituitary hormone secretion in mammals, with special reference to development

Author

Oliver, C., Boudouresque, F., Chautard, T., Jezová, D., Denis Becquet, Guillaume, V., Héry, F., Giraud, P., Grino, M., Becquet, Denis, Service d'endocrinologie, diabète et maladies métaboliques, Université de la Méditerranée - Aix-Marseille 2-Assistance Publique - Hôpitaux de Marseille (APHM)- Hôpital de la Timone [CHU - APHM] (TIMONE), Interactions cellulaires neuroendocriniennes (ICN), Université de la Méditerranée - Aix-Marseille 2-Centre National de la Recherche Scientifique (CNRS), Institut de biologie et chimie des protéines [Lyon] (IBCP), Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)

Subjects
Embryonic and Fetal Development, Neurotransmitter Agents, Pituitary Hormones, Sheep, Adrenal Cortex Hormones, [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, [SDV.NEU.NB] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, Animals, Humans, Amino Acids, Macaca mulatta, ComputingMilieux_MISCELLANEOUS, Rats
Abstract

International audience; no abstract

Published
1991

23. Insulin-induced hypoglycemia stimulates corticotropin-releasing factor and arginine vasopressin secretion into hydrophysical portal blood of conscious, unrestrained rams

Author

Caraty, Alain, Grino, Michel, Locatelli, Aude, Guillaume, V., Boudouresque, F., Conte-Devolx, B., Oliver, Charlotte, ProdInra, Migration, Unité de recherche Physiologie de la reproduction des mammifères domestiques, Nouzilly, and Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV] Life Sciences [q-bio], ADRENOCORTICOTROPINE, [SDV]Life Sciences [q-bio], CORTISOL, [INFO]Computer Science [cs], CRF, [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
1990

24. CRF and AVP secretion into rat and sheep hypophysial portal blood

Author

Oliver, Charlotte, Guillaume, V., Grino, Michel, Caraty, Alain, Conte-Devolx, B., Boudouresque, F., Unité de recherche Physiologie de la reproduction des mammifères domestiques, Nouzilly, Institut National de la Recherche Agronomique (INRA), D.K. Ludecke (Editeur), G.P. Chouros (Editeur), G. Tolis (Editeur), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], CRH, [SDV]Life Sciences [q-bio], RAT, [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
1990

35. Enkephalins in human pheochromocytoma biosynthesis and secretion.

Author

Giraud, P., Castanas, E., Oliver, C., Conte-Devolx, B., Boudouresque, F., Taquet, H., Orlando, M., Jaquet, P., Eiden, L., Cesselin, F., Gunz, G., and Trigano, M.

Abstract

Adrenal medulla has recently been shown to contain high concentrations of enkephalin immunoreactive peptides. In the present study, we report the levels of Methionine-Enkephalin (M-ENK) and Leucine-Enkephalin (L-ENK) in extracts from six cases of human pheochromocytoma. The molecular forms of M-ENK have been characterized by gel filtration chromatography. PRO-M-ENK mRNA extracted from one tumour has been estimated to represent 1% of total mRNA after in vitro translation. M-ENK immunoreactive peptides are secreted in the culture medium of dispersed cultured cells of human pheochromocytoma. This secretion is stimulated when nicotine (10−5M) is added to the medium. Nicotinic stimulation is inhibited by fentanyl (10−5M) or clonidine (10−4M). However, the level of plasma M-ENK in pheochromocytoma patients is not significantly different from normal patients. Data from Faden and Holaday(1) have established that naloxone (an opiate antagonist) a beneficial role in shock, but the origin and meaning of plasma M-ENK remain to be established. [ABSTRACT FROM PUBLISHER]

Published
1982
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37. Adrenal medullary opiate receptors. Pharmacological characterization in bovine adrenal medulla and a human pheochromocytoma.

Author

Castanas, E, Giraud, P, Audigier, Y, Drissi, R, Boudouresque, F, Conte-Devolx, B, and Oliver, C

Abstract

We have characterized the opiate binding sites on the membranes of bovine adrenal medulla and human pheochromocytoma, using 3H-labeled D-Ala2-D-Leu5-enkephalin ( [3H]DADLE), [3H]etorphine, and [3H]ethylketocyclazocine ( [3H]EKC). Binding was stereoselective in both membrane preparations. Association and dissociation kinetics showed that steady state was achieved after 20-25 min of incubation at 37 degrees. Saturation experiments were performed in the absence or in the presence of morphiceptin (1 microM), which masks the mu sites, D-Ser2-Leu-enkephalin-Thr6 (100 nM), which masks delta sites, or DADLE (5 microM), which was found to mask the delta, mu, and benzomorphan receptor. Taking into consideration the affinities of the three radioligands used (DADLE identifying the delta and mu sites when used in the nanomolar range; etorphine identifying the delta, mu, and benzomorphan sites; EKC identifying the delta, mu, kappa, and benzomorphan receptors) we have characterized pharmacologically the opiate sites present on bovine and human membranes. Human pheochromocytoma membranes contained (a) mu binding sites (15 fmoles/mg of protein, KD [3H]etorphine 1.0 nM, [3H]EKC 5.4 nM, [3H]DADLE 5.6 nM); (b) kappa sites (41 fmoles/mg of protein, KD [3H]EKC 1.0 nM); (c) benzomorphan sites (115 fmoles/mg of protein, KD [3H]etorphine and [3H]EKC 1.0 nM). On bovine membranes we have detected (a) delta binding sites (10 fmoles/mg of protein, KD [3H]DADLE 0.7 nM); (b) mu sites (24 fmoles/mg of protein, KD [3H]DADLE 2.9 nM, [3H]etorphine 0.2 nM, [3H]EKC 3.4 nM); (c) kappa sites (12 fmoles/mg of protein, KD [3H]EKC 0.4 nM); (d) benzomorphan sites (80 fmoles/mg of protein, KD [3H]etorphine 0.2 nM, [3H]EKC 1.3 nM); (e) a residual high-affinity (20 fmoles/mg of protein, KD 0.2 nM) site identified by [3H]etorphine in the presence of 5 microM DADLE. The relative proportions of benzomorphan sites were equal in both tissues (65% of the high-affinity sites) whereas kappa receptors were more abundant on human membranes (25%) than on bovine membranes (9% of the high-affinity sites).

Published
1984

38. Evidence for high peptide alpha-amidating activity in the pancrease from neonatal rats.

Author

Ouafik, L, Giraud, P, Salers, P, Dutour, A, Castanas, E, Boudouresque, F, and Oliver, C

Abstract

A high peptidylglycine alpha-amidating mono-oxygenase (PAMase) activity has been measured in the pancreas of neonatal rats. A significant fraction of this activity is contained in the beta cells of the islets of Langerhans and is colocalized with thyrotropin-releasing hormone (TRH) and its precursor in secretory granules. The ontogenetic variation of PAMase activity in the pancrease parallels that of TRH concentrations, suggesting that this enzymatic activity is directly related to TRH biosynthesis. In addition, PAMase activity is able to generate TRH when incubated with less than Glu-His-Pro-Gly, a tetrapeptide present as a repetitive sequence in the TRH precursor. The perinatal evolution of the TRH precursor levels in the pancreas is similar to that of PAMase activity (unpublished results). Thus, the neonatal rat pancreas offers an endocrine model in which the levels of a neuropeptide precursor and an enzyme activity, involved in the posttranslational modification of this precursor, are similarly regulated. Our results suggest also that a fraction of PAMase activity may be produced outside of the beta cells and related to the biosynthesis of COOH-terminally amidated peptide(s) other than TRH. The ontogenetic changes in PAMase activity imply that the synthesis of this peptide(s) is high during the neonatal period, decreasing thereafter.

Published
1987
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39. Corticotrophin-releasing factor immunoreactivity in the hypothalamus of the rat during the perinatal period

Author

Chatelain, A., Boudouresque, F., Chautard, T., Dupouyt, J. P., and Oliver, C.

Abstract

Corticotrophin-releasing factor-41 (CRF-41) immunoreactivity has been measured in hypothalamic extracts of fetal (on days 17, 19 and 21 of gestation), neonatal (1, 2, 3 and 4 weeks of age) and adult rats with a specific radioimmunoassay developed for synthetic rat CRF-41. The hypothalamic content (fmol) and concentration (fmol/mg protein) of immunoreactive CRF-41 gradually increased with age. Chromatography of hypothalamic extracts on Sephadex G-50 Fine showed one single peak of immunoreactive CRF-41 which co-eluted with synthetic rat CRF-41. The retention time of hypothalamic CRF-41 during high-performance liquid chromatography was identical to that of synthetic rat CRF-41 at all stages investigated. These results are consistent with the development of neurones containing CRF-41-like molecules in both the hypothalamus and the median eminence of the fetus, as well as with the hypothalamic control of the cortico-stimulating function of the pituitary gland as early as day 19 of gestation.J. Endocr. (1988) 119,59–64

Published
1988
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40. [Demonstration of a TRH precursor in the pancreas of the newborn rat]

Author

Ouafik, L, Dutour, A., Giraud, P., Boudouresque, F., Castanas, C, Oliver, C., Ouafik, L'Houcine, Laboratoire de Neuroendocrinologie Expérimentale, and Institut National de la Santé et de la Recherche Médicale (INSERM)

Subjects
MESH: Protein Precursors, endocrine system, MESH: Rats, endocrine system diseases, MESH: Molecular Weight, [SDV.CAN]Life Sciences [q-bio]/Cancer, MESH: Pancreas, MESH: Pyrrolidonecarboxylic Acid, MESH: Animals, Newborn, Pyrrolidonecarboxylic Acid, Rats, Molecular Weight, Animals, Newborn, [SDV.CAN] Life Sciences [q-bio]/Cancer, MESH: Thyrotropin-Releasing Hormone, Animals, MESH: Animals, Protein Precursors, Pancreas, Thyrotropin-Releasing Hormone, hormones, hormone substitutes, and hormone antagonists
Abstract

International audience; This study allows the indirect demonstration of a precursor for TRH in pancreatic extracts of 2-days old rats. The sequential treatment of these extracts with trypsin and carboxypeptidase A is followed by a large increase in Pyroglutamyl Histidine Proline (TRH-OH). The molecular weight of the protein that gives rise to TRH-OH after enzymatic treatment ranges between 30,000 and 40,000 daltons. During ontogenesis. TRH levels decrease earlier and more rapidly than that of TRH-precursor levels. These data suggest that changes in the processing of TRH-precursor play a role in the diminution of TRH concentrations that is observed during the first two weeks of life.

Published
1986

41. Developmental aspects of the hypothalamic‐pituitary‐adrenal axis in the rat

Author

Grino, M., Boudouresque, F., Chautard, T., Denis Becquet, Guillaume, V., Strbák, V., Oliver, C., Interactions cellulaires neuroendocriniennes (ICN), Université de la Méditerranée - Aix-Marseille 2-Centre National de la Recherche Scientifique (CNRS), Institut de biologie et chimie des protéines [Lyon] (IBCP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Service d'endocrinologie, diabète et maladies métaboliques, Université de la Méditerranée - Aix-Marseille 2-Assistance Publique - Hôpitaux de Marseille (APHM)- Hôpital de la Timone [CHU - APHM] (TIMONE), and Becquet, Denis

Subjects
Hypothalamo-Hypophyseal System, Fetus, Pregnancy, [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, [SDV.NEU.NB] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, Animals, Pituitary-Adrenal System, Female, ComputingMilieux_MISCELLANEOUS, Rats
Abstract

International audience; no abstract

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