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Biochar has been recognized for its potential to improve the fertility soils by reducing the reliance on chemical fertilizers, mitigating carbon emissions, and fostering soil microbial growth. This study aimed to evaluate the impact of biochar addition on the physicochemical properties of arid and semi-arid soils containing microplastics, while also assessing its effect on Barley (Hordeum vulgare) yield under drought stress. The experiment was conducted in a glass greenhouse. Plastic pots containing 3 kg of soil were each planted with 6 barley grains. Biochar was applied at three doses (B0 = 0 g biochar/kg soil, B1 = 6 g biochar/kg soil, B2 = 10 g biochar/kg soil), while microplastics were added at three levels (M0 = Control without microplastics, M1 = 0.5 g/kg soil, and M2 = 1 g/kg soil) on the same sowing date. Water stress was induced when the plants reached the four-leaf stage. ANOVAs and Tukey post-hoc tests were employed for multiple mean comparisons of soil and plant parameters. Drought stress and microplastics negatively influenced soil parameters namely soil moisture, organic carbon, and nitrates, while also affecting electrical conductivity and pH. Biochar exhibited minimal effect on soil properties but significantly altered pH, nitrates, and total CaCO 3 . Plant chlorophyll levels decreased under stress, particularly with microplastic dose M1. However, biochar and microplastics enhanced chlorophyll a content, except for dose B1 of biochar, which leads to a decrease in chlorophyll b (0.91 ± 0.138 μg/g FM). Microplastics, at dose M2, improved chlorophyll b content (1.11 ± 0.090 μg/g FM). Aboveground biomass, leaf area, and yield were generally unaffected by tested stresses. Nonetheless, barley grain yield decreased in biochar and microplastic dose M1 (0.47 ± 0.108 g/plant), while it improved with microplastic dose M2 (0.65 ± 0.168 g/plant). Leaf relative water content increased under water stress and microplastics but not with biochar alone. Interaction between microplastics and biochar enhanced plant water content. Drought stress and microplastics diminished soil parameters, whereas biochar lowered nitrates and pH without significantly affecting soil organic carbon. Plant productivity parameters generally exhibited no significant change under water stress, microplastics, or biochar, except for yield and chlorophyll pigments., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

The use of biochar has recently garnered significant attention as an agricultural management technique highly endorsed by the scientific community. Biochar, owing to its high carbon content, contributes to increased organic matter storage in the soil, consequently enhancing crop growth. This study aimed to elucidate changes in physicochemical soil fertility and durum wheat ( Triticum durum ) var. Vitron production under the influence of three biochar doses (0 g/kg, 5 g/kg, and 15 g/kg of soil) in combination with varying levels of drought stress (100 %, 80 %, 40 %, and 20 % of field capacity 'FC'). Notably, we observed a substantial increase in all physicochemical soil parameters, except for active calcium carbonate equivalent (ACCE), which displayed lower values (8.78 ± 1.43 %) in soils treated with biochar compared to control soil (15.69 ± 4.03 %). The biochar dose of 5 g/kg yielded the highest moisture content (8.81 %) and pH value (7.83). However, the highest organic matter content (4.89 ± 0.17 %) and total calcium carbonate equivalent 'TCCE' (3.67 ± 0.48 %) were observed with the dose 15 g/kg. Nevertheless, regarding plant growth, no improvements were observed in terms of height and above-ground biomass (AGB). Conversely, leaf surface area exhibited significant changes with biochar application, along with an increase in chlorophyll pigment content. On the other hand, drought stress significantly hindered plant height, AGB, and leaf water reserves, resulting in values of 13.48 ± 1.60 cm, 1.57 ± 0.31g/plant, and 41.79 ± 1.67 %, respectively. The interaction between biochar and water stress appeared to mitigate and limit the impact of stress. Notably, an enhancement in organic matter storage and soil water reserves was observed. For example, the moisture content in the control soil was 6.95 %, while it increased to 12.76 % for 15g biochar/kg and 80 % FC. A similar trend was observed for organic matter, TCCE, and electrical conductivity. This effect positively influenced chlorophyll a and b content, as well as leaf water content. However, when stress was combined with biochar amendment, plant height and AGB decreased. The addition of biochar improved soil fertility and physiological parameters of wheat plants. Nevertheless, when combined with water stress, especially in cases of reduced water reserves, productivity did not witness any significant improvements., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2023 The Authors.)

With soil fertility loss reached a critical state in arid and semi-arid regions, farmers are constrained to use mineral fertilizers, which are costly, non-eco-friendly and less effective in improving soil fertility than organic fertilizers such as dewatered sewage sludge (SS) and poultry manure (PM). In this regard, the current study aimed to highlight through experiments the positive effect of SS and PM applications on soil fertility and durum wheat growth. It targeted to demonstrate the safe and wise use of organic fertilization while assessing heavy metals in both soil and plant. The experiment was carried out in two batches of thirty-two pots, one for each treatment (SS and PM), in addition to the control with no fertilization. SS and PM were applied separately in three doses (D1 = 50 g, D2 = 100 g, and D3 = 200 g DM fertilizer/pot). The applications of both SS and PM induced a significant increase in plant-available phosphorus, organic matter, nitrates, moisture and electrical conductivity in soil, where these improvements were higher in PM compared to SS treatment. A significant accumulation of proline associated with an increase in biomass that were both proportional with fertilizer dose levels. Our findings revealed a loss in relative water content and leaf area of the plant. Correlations showed several significant relationships between soil parameters studied. The dose D2 of each fertilizer was the most efficient to improving both soil properties and plant components. Plant zinc concentration increased significantly with increase in soil zinc in PM amendments, however it decreased in SS. These relationships were not significant in copper for the two fertilizers. Both SS and PM improved soil fertility and plant growth compared to the control, thus this practice is a promising solution to tackle soil fertility loss and low production in drylands., Competing Interests: The authors declare no conflict of interest., (© 2023 The Author(s).)

The fertilization using sewage sludge (SS) and/or SS-derived products have been extensively studied and known to increase crop yield as soil nutrients and plant growth are improved. This study aimed to evaluate two SS application methods (i.e. mulching and mixing with the soil) on soil fertility parameters and the productivity of cereal crops. It compared the effect of SS fertilization methods on changes in soil physicochemical parameters in order to highlight the application mode which gives the best agronomic values and sustains soil productivity. Foliar surface, grain starch content and grain yield of durum wheat (Triticum durum) were determined in plants grown in plastic planters for different fertilization treatments (SS-mulched, SS-mixed, urea, and unfertilized). Each SS treatment was applied in three levels (SS1 = 1% w/w ratio, SS2 = 4%, SS3 = 8.3%). The application of SS improved all soil properties compared to the control and urea, with the SS mulching treatment was the best. The significant improvement of soil fertility was confirmed by soil C:P ratio which indicated a good soil mineralization status, in particular under the screen formed by mulching that helped to conserve high soil moisture for optimizing plant growth. Soil calcium accumulated in greater amount in biosolid-soil mixtures than in SS-mulched soils. Regardless of SS doses, the highest crop grain yields were obtained with the SS mulch treatments. Mulching SS, compared to SS-mixed soils, brings better results in terms of improving soil fertility and yielding high productions. The applicable of this method is also easy in the field and/or large-scale cultures., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

In arid and semi-arid areas, low soil fertility and water deficit considerably limit crop production. The use of sewage sludge as an organic amendment could contribute to the improvement of soil fertility and hence the agronomic production. The study aims to highlight the behaviour of durum wheat to the application of sewage sludge associated with water stress. The assessment focused on morphophysiological parameters of the wheat plant and yield. Under greenhouse conditions, the variety Mohamed Ben Bachir was treated by four water stress levels (100 %, 80 %, 50 % and 30 %). Each stress level comprised five fertilizer treatments: 20, 50 and 100 t/ha of dry sludge, 35 kg/ha of urea, and a control with no fertilization. Results revealed a significant loss in water content and chlorophyll a in leaves. Water stress negatively affected the development of wheat plants by reducing significantly seed yield, leaf area and biomass produced. Plant's responses to water stress manifested by an accumulation of proline and a decrease in total phosphorus. However, the increasing doses of sewage sludge limited the effect of water stress. Our findings showed an increase in the amount of chlorophyll pigments, leaf area, total phosphorus, biomass and yield. In addition, excessive accumulation of proline (1.11 ± 1.03 µg/g DM) was recorded as a result of the high concentration of sludge (100 t/ha DM). The application of sewage sludge is beneficial for the wheat crop, but the high accumulation of proline in plants treated with high dose of sludge suggests to properly consider this fact. The application of sludge should be used with caution in soils where water is limited. Because the combined effect of these two factors could result in a fatal osmotic stress to crop development.

The structures of the two predominant metabolites (M4 and M5) of RVX-208, observed both in in vitro human and animal liver microsomal incubations, as well as in plasma from animal in vivo studies, were determined. A panel of biocatalytic systems was tested to identify biocatalysts suitable for milligram scale production of metabolite M4 from RVX-208. Rabbit liver S9 fraction was selected as the most suitable system, primarily based on pragmatic metrics such as catalyst cost and estimated yield of M4 (∼55%). Glucuronidation of RVX-208 catalyzed by rabbit liver S9 fraction was optimized to produce M4 in amounts sufficient for structural characterization. Structural studies using LC/MS/MS analysis and (1)H NMR spectroscopy showed the formation of a glycosidic bond between the primary hydroxyl group of RVX-208 and glucuronic acid. NMR results suggested that the glycosidic bond has the β-anomeric configuration. A synthetic sample of M4 confirmed the proposed structure. Metabolite M5, hypothesized to be the carboxylate of RVX-208, was prepared using human liver microsomes, purified by HPLC, and characterized by LC/MS/MS and (1)H NMR. The structure was confirmed by comparison to a synthetic sample. Both samples confirmed M5 as a product of oxidation of primary hydroxyl group of RVX-208 to carboxylic acid., (Copyright © 2013 Elsevier Masson SAS. All rights reserved.)