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6 results on '"Bouchet, Pierre-Etienne"'

2. Blue-light induced accumulation of reactive oxygen species is a consequence of the Drosophila cryptochrome photocycle

Author

El-Esawi, Mohamed, d'Harlingue, Alain, Bouchet, Pierre-Etienne, Witczak, Jacques, Ritz, Thorsten, Klarsfeld, Andre, Birman, Serge, Usselman, Robert J., Hoecker, Ute, Martino, Carlos F., Ahmad, Margaret, Arthaut, Louis-David, Jourdan, Nathalie, Mteyrek, Ali, Procopio, Maria, Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Adaptation Biologique et Vieillissement = Biological Adaptation and Ageing (B2A), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Plasticité du Cerveau Brain Plasticity (UMR 8249) (PdC), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Johns Hopkins University (JHU), Photobiologie (PHOTO), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Paris Seine (IBPS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), University of California [Irvine] (UC Irvine), University of California (UC), Cluster of Excellence on Plant Sciences (CEPLAS), Botanical Institute, University of Cologne, Florida Institute of Technology [Melbourne], Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Air Force Office of Sponsored Research [FA9550-14-0-0409], Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), and Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Luminescence, Light, Arthropoda, Photoperiod, [SDV]Life Sciences [q-bio], lcsh:Medicine, Spodoptera, Research and Analysis Methods, Biochemistry, Fluorescence, Cell Line, Redox Signaling, Model Organisms, Cell Signaling, Electrochemistry, Animals, Humans, Post-Translational Modification, lcsh:Science, Flavin, Physics, Electromagnetic Radiation, Drosophila Melanogaster, lcsh:R, Chemical Reactions, Organisms, Biology and Life Sciences, Proteins, Cell Biology, Animal Models, Cell Cultures, Invertebrates, Cryptochromes, Insects, Kinetics, Chemistry, Experimental Organism Systems, Physical Sciences, Quantum Theory, Drosophila, lcsh:Q, Biological Cultures, Reactive Oxygen Species, Research Article, Signal Transduction, Oxidation-Reduction Reactions
Abstract

International audience; Cryptochromes are evolutionarily conserved blue-light absorbing flavoproteins which participate in many important cellular processes including in entrainment of the circadian clock in plants, Drosophila and humans. Drosophila melanogaster cryptochrome (DmCry) absorbs light through a flavin (FAD) cofactor that undergoes photoreduction to the anionic radical (FAD(center dot-)) redox state both in vitro and in vivo. However, recent efforts to link this photoconversion to the initiation of a biological response have remained controversial. Here, we show by kinetic modeling of the DmCry photocycle that the fluence dependence, quantum yield, and half-life of flavin redox state interconversion are consistent with the anionic radical (FAD(center dot-)) as the signaling state in vivo. We show by fluorescence detection techniques that illumination of purified DmCry results in enzymatic conversion of molecular oxygen (O-2) to reactive oxygen species (ROS). We extend these observations in living cells to demonstrate transient formation of superoxide (O-2(center dot-)), and accumulation of hydrogen peroxide (H2O2) in the nucleus of insect cell cultures upon DmCry illumination. These results define the kinetic parameters of the Drosophila cryptochrome photocycle and support light-driven electron transfer to the flavin in DmCry signaling. They furthermore raise the intriguing possibility that light-dependent formation of ROS as a byproduct of the cryptochrome photocycle may contribute to its signaling role.

Published
2017
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3. Blue-light induced accumulation of reactive oxygen species is a consequence of the Drosophila cryptochrome photocycle

Author

Arthaut, Louis-David, primary, Jourdan, Nathalie, additional, Mteyrek, Ali, additional, Procopio, Maria, additional, El-Esawi, Mohamed, additional, d’Harlingue, Alain, additional, Bouchet, Pierre-Etienne, additional, Witczak, Jacques, additional, Ritz, Thorsten, additional, Klarsfeld, André, additional, Birman, Serge, additional, Usselman, Robert J., additional, Hoecker, Ute, additional, Martino, Carlos F., additional, and Ahmad, Margaret, additional

Published
2017
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4. Blue-light induced accumulation of reactive oxygen species is a consequence of the Drosophila cryptochrome photocycle

Author

Arthaut, Louis-David, Jourdan, Nathalie, Mteyrek, Ali, Procopio, Maria, El-Esawi, Mohamed, d'Harlingue, Alain, Bouchet, Pierre-Etienne, Witczak, Jacques, Ritz, Thorsten, Klarsfeld, Andre, Birman, Serge, Usselman, Robert J., Hoecker, Ute, Martino, Carlos F., Ahmad, Margaret, Arthaut, Louis-David, Jourdan, Nathalie, Mteyrek, Ali, Procopio, Maria, El-Esawi, Mohamed, d'Harlingue, Alain, Bouchet, Pierre-Etienne, Witczak, Jacques, Ritz, Thorsten, Klarsfeld, Andre, Birman, Serge, Usselman, Robert J., Hoecker, Ute, Martino, Carlos F., and Ahmad, Margaret

Abstract

Cryptochromes are evolutionarily conserved blue-light absorbing flavoproteins which participate in many important cellular processes including in entrainment of the circadian clock in plants, Drosophila and humans. Drosophila melanogaster cryptochrome (DmCry) absorbs light through a flavin (FAD) cofactor that undergoes photoreduction to the anionic radical (FAD(center dot-)) redox state both in vitro and in vivo. However, recent efforts to link this photoconversion to the initiation of a biological response have remained controversial. Here, we show by kinetic modeling of the DmCry photocycle that the fluence dependence, quantum yield, and half-life of flavin redox state interconversion are consistent with the anionic radical (FAD(center dot-)) as the signaling state in vivo. We show by fluorescence detection techniques that illumination of purified DmCry results in enzymatic conversion of molecular oxygen (O-2) to reactive oxygen species (ROS). We extend these observations in living cells to demonstrate transient formation of superoxide (O-2(center dot-)), and accumulation of hydrogen peroxide (H2O2) in the nucleus of insect cell cultures upon DmCry illumination. These results define the kinetic parameters of the Drosophila cryptochrome photocycle and support light-driven electron transfer to the flavin in DmCry signaling. They furthermore raise the intriguing possibility that light-dependent formation of ROS as a byproduct of the cryptochrome photocycle may contribute to its signaling role.

Published
2017

6. Blue‐light dependent reactive oxygen species formation by Arabidopsis cryptochrome may define a novel evolutionarily conserved signaling mechanism

Author

Consentino, Laurent, primary, Lambert, Stefan, additional, Martino, Carlos, additional, Jourdan, Nathalie, additional, Bouchet, Pierre‐Etienne, additional, Witczak, Jacques, additional, Castello, Pablo, additional, El‐Esawi, Mohamed, additional, Corbineau, Francoise, additional, d'Harlingue, Alain, additional, and Ahmad, Margaret, additional

Published
2015
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