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1. Advances and Challenges in Cell Biology for Cultured Meat

Author

Sub Cell Biology, Cell Biology, Neurobiology and Biophysics, Martins, Beatriz, Bister, Arthur, Dohmen, Richard G.J., Gouveia, Maria Ana, Hueber, Rui, Melzener, Lea, Messmer, Tobias, Papadopoulos, Joanna, Pimenta, Joana, Raina, Dhruv, Schaeken, Lieke, Shirley, Sara, Bouchet, Benjamin P., Flack, Joshua E., Sub Cell Biology, Cell Biology, Neurobiology and Biophysics, Martins, Beatriz, Bister, Arthur, Dohmen, Richard G.J., Gouveia, Maria Ana, Hueber, Rui, Melzener, Lea, Messmer, Tobias, Papadopoulos, Joanna, Pimenta, Joana, Raina, Dhruv, Schaeken, Lieke, Shirley, Sara, Bouchet, Benjamin P., and Flack, Joshua E.

Published
2024

2. Advances and Challenges in Cell Biology for Cultured Meat

Author

Martins, Beatriz, Bister, Arthur, Dohmen, Richard G J, Gouveia, Maria Ana, Hueber, Rui, Melzener, Lea, Messmer, Tobias, Papadopoulos, Joanna, Pimenta, Joana, Raina, Dhruv, Schaeken, Lieke, Shirley, Sara, Bouchet, Benjamin P, Flack, Joshua E, Martins, Beatriz, Bister, Arthur, Dohmen, Richard G J, Gouveia, Maria Ana, Hueber, Rui, Melzener, Lea, Messmer, Tobias, Papadopoulos, Joanna, Pimenta, Joana, Raina, Dhruv, Schaeken, Lieke, Shirley, Sara, Bouchet, Benjamin P, and Flack, Joshua E

Abstract

Cultured meat is an emerging biotechnology that aims to produce meat from animal cell culture, rather than from the raising and slaughtering of livestock, on environmental and animal welfare grounds. The detailed understanding and accurate manipulation of cell biology are critical to the design of cultured meat bioprocesses. Recent years have seen significant interest in this field, with numerous scientific and commercial breakthroughs. Nevertheless, these technologies remain at a nascent stage, and myriad challenges remain, spanning the entire bioprocess. From a cell biological perspective, these include the identification of suitable starting cell types, tuning of proliferation and differentiation conditions, and optimization of cell-biomaterial interactions to create nutritious, enticing foods. Here, we discuss the key advances and outstanding challenges in cultured meat, with a particular focus on cell biology, and argue that solving the remaining bottlenecks in a cost-effective, scalable fashion will require coordinated, concerted scientific efforts. Success will also require solutions to nonscientific challenges, including regulatory approval, consumer acceptance, and market feasibility. However, if these can be overcome, cultured meat technologies can revolutionize our approach to food. Expected final online publication date for the , Volume 12 is February 2024. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

Published
2024

3. Advances and Challenges in Cell Biology for Cultured Meat

Author

Martins, Beatriz, primary, Bister, Arthur, additional, Dohmen, Richard G.J., additional, Gouveia, Maria Ana, additional, Hueber, Rui, additional, Melzener, Lea, additional, Messmer, Tobias, additional, Papadopoulos, Joanna, additional, Pimenta, Joana, additional, Raina, Dhruv, additional, Schaeken, Lieke, additional, Shirley, Sara, additional, Bouchet, Benjamin P., additional, and Flack, Joshua E., additional

Published
2023
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8. Microtubules in 3D cell motility

Author

Bouchet, Benjamin P, Akhmanova, Anna, Sub Cell Biology, Celbiologie, Sub Cell Biology, and Celbiologie

Subjects
0301 basic medicine, Morphogenesis, Regulator, Motility, Review, Biology, Research Support, Microtubules, Imaging, Extracellular matrix, 03 medical and health sciences, 3D cell culture, Imaging, Three-Dimensional, Microtubule, Cell Movement, Journal Article, Animals, Humans, Cytoskeleton, Non-U.S. Gov't, Research Support, Non-U.S. Gov't, Cell migration, Cell Biology, Cell biology, Extracellular Matrix, 030104 developmental biology, Cellular Microenvironment, Three-Dimensional
Abstract

Three-dimensional (3D) cell motility underlies essential processes, such as embryonic development, tissue repair and immune surveillance, and is involved in cancer progression. Although the cytoskeleton is a well-studied regulator of cell migration, most of what we know about its functions originates from studies conducted in two-dimensional (2D) cultures. This research established that the microtubule network mediates polarized trafficking and signaling that are crucial for cell shape and movement in 2D. In parallel, developments in light microscopy and 3D cell culture systems progressively allowed to investigate cytoskeletal functions in more physiologically relevant settings. Interestingly, several studies have demonstrated that microtubule involvement in cell morphogenesis and motility can differ in 2D and 3D environments. In this Commentary, we discuss these differences and their relevance for the understanding the role of microtubules in cell migration in vivo. We also provide an overview of microtubule functions that were shown to control cell shape and motility in 3D matrices and discuss how they can be investigated further by using physiologically relevant models.

Published
2017

9. Microtubules in 3D cell motility

Author

Sub Cell Biology, Celbiologie, Bouchet, Benjamin P, Akhmanova, Anna, Sub Cell Biology, Celbiologie, Bouchet, Benjamin P, and Akhmanova, Anna

Published
2017

11. Molecular Pathway of Microtubule Organization at the Golgi Apparatus

Author

Wu, Jingchao, primary, de Heus, Cecilia, additional, Liu, Qingyang, additional, Bouchet, Benjamin P., additional, Noordstra, Ivar, additional, Jiang, Kai, additional, Hua, Shasha, additional, Martin, Maud, additional, Yang, Chao, additional, Grigoriev, Ilya, additional, Katrukha, Eugene A., additional, Altelaar, A.F. Maarten, additional, Hoogenraad, Casper C., additional, Qi, Robert Z., additional, Klumperman, Judith, additional, and Akhmanova, Anna, additional

Published
2016
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12. Rb and FZR1/Cdh1 determine CDK4/6-cyclin D requirement in C. elegans and human cancer cells

Author

The, Inge, Ruijtenberg, Suzan, Bouchet, Benjamin P, Cristobal Gonzalez de Durana, Alba, Prinsen, Martine B W, van Mourik, Tim, Koreth, John, Xu, Huihong, Heck, Albert J R, Akhmanova, Anna, Cuppen, Edwin, Boxem, Mike, Munoz Murillo, Ara, van den Heuvel, Sander, Developmental Biology, Celbiologie, Biomolecular Mass Spectrometry and Proteomics, Sub Developmental Biology, Sub Cell Biology, Sub Biomol.Mass Spectrometry & Proteom., Sub Biomol.Mass Spect. and Proteomics, Sub Inorganic Chemistry and Catalysis, Hubrecht Institute for Developmental Biology and Stem Cell Research, NIH Office of Research Infrastructure Programs, Unión Europea, Netherlands Organization for Scientific Research, Developmental Biology, Celbiologie, Biomolecular Mass Spectrometry and Proteomics, Sub Developmental Biology, Sub Cell Biology, Sub Biomol.Mass Spectrometry & Proteom., Sub Biomol.Mass Spect. and Proteomics, and Sub Inorganic Chemistry and Catalysis

Subjects
Cell division, Chemistry(all), Cyclin D, General Physics and Astronomy, medicine.disease_cause, Retinoblastoma Protein, environment and public health, Biochemistry, Cdh1 Proteins, Mass Spectrometry, 0302 clinical medicine, Non-U.S. Gov't, Cyclin, 0303 health sciences, Mutation, Multidisciplinary, integumentary system, Kinase, Research Support, Non-U.S. Gov't, Cell Cycle, Cell cycle, 3. Good health, 030220 oncology & carcinogenesis, Gene Knockdown Techniques, Phosphorylation, biological phenomena, cell phenomena, and immunity, Molecular Sequence Data, Biology, Physics and Astronomy(all), Research Support, Article, General Biochemistry, Genetics and Molecular Biology, N.I.H, 03 medical and health sciences, Research Support, N.I.H., Extramural, Cell Line, Tumor, medicine, Journal Article, Animals, Humans, Immunoprecipitation, Caenorhabditis elegans, Caenorhabditis elegans Proteins, 030304 developmental biology, Base Sequence, Biochemistry, Genetics and Molecular Biology(all), Cyclin-Dependent Kinase 4, Extramural, General Chemistry, Cyclin-Dependent Kinase 6, Sequence Analysis, DNA, Molecular biology, Repressor Proteins, enzymes and coenzymes (carbohydrates), HEK293 Cells, Microscopy, Fluorescence, Multiprotein Complexes, Cancer cell, biology.protein, Genetics and Molecular Biology(all)
Abstract

Cyclin-dependent kinases 4 and 6 (CDK4/6) in complex with D-type cyclins promote cell cycle entry. Most human cancers contain overactive CDK4/6-cyclin D, and CDK4/6-specific inhibitors are promising anti-cancer therapeutics. Here, we investigate the critical functions of CDK4/6-cyclin D kinases, starting from an unbiased screen in the nematode Caenorhabditis elegans. We found that simultaneous mutation of lin-35, a retinoblastoma (Rb)-related gene, and fzr-1, an orthologue to the APC/C co-activator Cdh1, completely eliminates the essential requirement of CDK4/6-cyclin D (CDK-4/CYD-1) in C. elegans. CDK-4/CYD-1 phosphorylates specific residues in the LIN-35 Rb spacer domain and FZR-1 amino terminus, resembling inactivating phosphorylations of the human proteins. In human breast cancer cells, simultaneous knockdown of Rb and FZR1 synergistically bypasses cell division arrest induced by the CDK4/6-specific inhibitor PD-0332991. Our data identify FZR1 as a candidate CDK4/6-cyclin D substrate and point to an APC/CFZR1 activity as an important determinant in response to CDK4/6-inhibitors., In most human tumours, the cell cycle regulators Cdk4/6-cyclinD are overactive. Here the authors use C. elegans as a model system to identify downstream regulators that are critical in the response of tumour cells to Cdk4/6 inhibitors.

Published
2015

13. Mesenchymal Cell Invasion Requires Cooperative Regulation of Persistent Microtubule Growth by SLAIN2 and CLASP1

Author

Bouchet, Benjamin P, Noordstra, Ivar, van Amersfoort, Miranda, Katrukha, Eugene A, Ammon, York-Christoph, Ter Hoeve, Natalie D, Hodgson, Louis, Dogterom, Marileen, Derksen, Patrick W B, Akhmanova, Anna, Bouchet, Benjamin P, Noordstra, Ivar, van Amersfoort, Miranda, Katrukha, Eugene A, Ammon, York-Christoph, Ter Hoeve, Natalie D, Hodgson, Louis, Dogterom, Marileen, Derksen, Patrick W B, and Akhmanova, Anna

Published
2016

14. Molecular Pathway of Microtubule Organization at the Golgi Apparatus

Author

Wu, Jingchao, de Heus, Cecilia, Liu, Qingyang, Bouchet, Benjamin P, Noordstra, Ivar, Jiang, Kai, Hua, Shasha, Martin, Maud, Yang, Chao, Grigoriev, Ilya, Katrukha, Eugene A, Altelaar, A F Maarten, Hoogenraad, Casper C, Qi, Robert Z, Klumperman, Judith, Akhmanova, Anna, Wu, Jingchao, de Heus, Cecilia, Liu, Qingyang, Bouchet, Benjamin P, Noordstra, Ivar, Jiang, Kai, Hua, Shasha, Martin, Maud, Yang, Chao, Grigoriev, Ilya, Katrukha, Eugene A, Altelaar, A F Maarten, Hoogenraad, Casper C, Qi, Robert Z, Klumperman, Judith, and Akhmanova, Anna

Published
2016

15. Molecular Pathway of Microtubule Organization at the Golgi Apparatus

Author

Sub Cell Biology, Sub Biomol.Mass Spect. and Proteomics, Celbiologie, Biomolecular Mass Spectrometry and Proteomics, Wu, Jingchao, de Heus, Cecilia, Liu, Qingyang, Bouchet, Benjamin P, Noordstra, Ivar, Jiang, Kai, Hua, Shasha, Martin, Maud, Yang, Chao, Grigoriev, Ilya, Katrukha, Eugene A, Altelaar, A F Maarten, Hoogenraad, Casper C, Qi, Robert Z, Klumperman, Judith, Akhmanova, Anna, Sub Cell Biology, Sub Biomol.Mass Spect. and Proteomics, Celbiologie, Biomolecular Mass Spectrometry and Proteomics, Wu, Jingchao, de Heus, Cecilia, Liu, Qingyang, Bouchet, Benjamin P, Noordstra, Ivar, Jiang, Kai, Hua, Shasha, Martin, Maud, Yang, Chao, Grigoriev, Ilya, Katrukha, Eugene A, Altelaar, A F Maarten, Hoogenraad, Casper C, Qi, Robert Z, Klumperman, Judith, and Akhmanova, Anna

Published
2016

16. Mesenchymal Cell Invasion Requires Cooperative Regulation of Persistent Microtubule Growth by SLAIN2 and CLASP1

Author

Celbiologie, Sub Cell Biology, Bouchet, Benjamin P, Noordstra, Ivar, van Amersfoort, Miranda, Katrukha, Eugene A, Ammon, York-Christoph, Ter Hoeve, Natalie D, Hodgson, Louis, Dogterom, Marileen, Derksen, Patrick W B, Akhmanova, Anna, Celbiologie, Sub Cell Biology, Bouchet, Benjamin P, Noordstra, Ivar, van Amersfoort, Miranda, Katrukha, Eugene A, Ammon, York-Christoph, Ter Hoeve, Natalie D, Hodgson, Louis, Dogterom, Marileen, Derksen, Patrick W B, and Akhmanova, Anna

Published
2016

17. Molecular Pathway of Microtubule Organization at the Golgi Apparatus

Author

Celbiologie, Biomolecular Mass Spectrometry and Proteomics, Sub Cell Biology, Sub Biomol.Mass Spect. and Proteomics, Wu, Jingchao, de Heus, Cecilia, Liu, Qingyang, Bouchet, Benjamin P, Noordstra, Ivar, Jiang, Kai, Hua, Shasha, Martin, Maud, Yang, Chao, Grigoriev, Ilya, Katrukha, Eugene A, Altelaar, A F Maarten, Hoogenraad, Casper C, Qi, Robert Z, Klumperman, Judith, Akhmanova, Anna, Celbiologie, Biomolecular Mass Spectrometry and Proteomics, Sub Cell Biology, Sub Biomol.Mass Spect. and Proteomics, Wu, Jingchao, de Heus, Cecilia, Liu, Qingyang, Bouchet, Benjamin P, Noordstra, Ivar, Jiang, Kai, Hua, Shasha, Martin, Maud, Yang, Chao, Grigoriev, Ilya, Katrukha, Eugene A, Altelaar, A F Maarten, Hoogenraad, Casper C, Qi, Robert Z, Klumperman, Judith, and Akhmanova, Anna

Published
2016

18. Talin-KANK1 interaction controls the recruitment of cortical microtubule stabilizing complexes to focal adhesions

Author

Biomolecular Mass Spectrometry and Proteomics, Sub Cell Biology, Sub Biomol.Mass Spect. and Proteomics, Sub Biomol.Mass Spectrometry & Proteom., Cell Biology, Neurobiology and Biophysics, Bouchet, Benjamin P, Gough, Rosemarie E, Ammon, York-Christoph, van de Willige, Dieudonnée, Post, Harm, Jacquemet, Guillaume, Altelaar, Maarten, Heck, Albert Jr, Goult, Ben T, Akhmanova, Anna, Biomolecular Mass Spectrometry and Proteomics, Sub Cell Biology, Sub Biomol.Mass Spect. and Proteomics, Sub Biomol.Mass Spectrometry & Proteom., Cell Biology, Neurobiology and Biophysics, Bouchet, Benjamin P, Gough, Rosemarie E, Ammon, York-Christoph, van de Willige, Dieudonnée, Post, Harm, Jacquemet, Guillaume, Altelaar, Maarten, Heck, Albert Jr, Goult, Ben T, and Akhmanova, Anna

Published
2016

19. Mesenchymal Cell Invasion Requires Cooperative Regulation of Persistent Microtubule Growth by SLAIN2 and CLASP1

Author

Pathologie Groep Derksen, Cancer, Bouchet, Benjamin P, Noordstra, Ivar, van Amersfoort, Miranda, Katrukha, Eugene A, Ammon, York-Christoph, Ter Hoeve, Natalie D, Hodgson, Louis, Dogterom, Marileen, Derksen, Patrick W B, Akhmanova, Anna, Pathologie Groep Derksen, Cancer, Bouchet, Benjamin P, Noordstra, Ivar, van Amersfoort, Miranda, Katrukha, Eugene A, Ammon, York-Christoph, Ter Hoeve, Natalie D, Hodgson, Louis, Dogterom, Marileen, Derksen, Patrick W B, and Akhmanova, Anna

Published
2016

20. Molecular Pathway of Microtubule Organization at the Golgi Apparatus

Author

CMM Groep Klumperman, TN groep Joëls, CMM Sectie Celbiologie, Cancer, Regenerative Medicine and Stem Cells, Wu, Jingchao, de Heus, Cecilia, Liu, Qingyang, Bouchet, Benjamin P, Noordstra, Ivar, Jiang, Kai, Hua, Shasha, Martin, Maud, Yang, Chao, Grigoriev, Ilya, Katrukha, Eugene A, Altelaar, A F Maarten, Hoogenraad, Casper C, Qi, Robert Z, Klumperman, Judith, Akhmanova, Anna, CMM Groep Klumperman, TN groep Joëls, CMM Sectie Celbiologie, Cancer, Regenerative Medicine and Stem Cells, Wu, Jingchao, de Heus, Cecilia, Liu, Qingyang, Bouchet, Benjamin P, Noordstra, Ivar, Jiang, Kai, Hua, Shasha, Martin, Maud, Yang, Chao, Grigoriev, Ilya, Katrukha, Eugene A, Altelaar, A F Maarten, Hoogenraad, Casper C, Qi, Robert Z, Klumperman, Judith, and Akhmanova, Anna

Published
2016

21. Molecular Pathway of Microtubule Organization at the Golgi Apparatus

Author

Cell Biology, Neurobiology and Biophysics, Biomolecular Mass Spectrometry and Proteomics, Sub Cell Biology, Sub Biomol.Mass Spect. and Proteomics, Wu, Jingchao, de Heus, Cecilia, Liu, Qingyang, Bouchet, Benjamin P, Noordstra, Ivar, Jiang, Kai, Hua, Shasha, Martin, Maud, Yang, Chao, Grigoriev, Ilya, Katrukha, Eugene A, Altelaar, A F Maarten, Hoogenraad, Casper C, Qi, Robert Z, Klumperman, Judith, Akhmanova, Anna, Cell Biology, Neurobiology and Biophysics, Biomolecular Mass Spectrometry and Proteomics, Sub Cell Biology, Sub Biomol.Mass Spect. and Proteomics, Wu, Jingchao, de Heus, Cecilia, Liu, Qingyang, Bouchet, Benjamin P, Noordstra, Ivar, Jiang, Kai, Hua, Shasha, Martin, Maud, Yang, Chao, Grigoriev, Ilya, Katrukha, Eugene A, Altelaar, A F Maarten, Hoogenraad, Casper C, Qi, Robert Z, Klumperman, Judith, and Akhmanova, Anna

Published
2016

22. Talin-KANK1 interaction controls the recruitment of cortical microtubule stabilizing complexes to focal adhesions

Author

Bouchet, Benjamin P., Gough, Rosemarie E., Ammon, York-Christoph, van de Willige, Dieudonnee, Post, Harm, Jacquemet, Guillaume, Altelaar, A.F. Maarten, Heck, Albert J.R., Goult, Benjamin T., Akhmanova, Anna, Bouchet, Benjamin P., Gough, Rosemarie E., Ammon, York-Christoph, van de Willige, Dieudonnee, Post, Harm, Jacquemet, Guillaume, Altelaar, A.F. Maarten, Heck, Albert J.R., Goult, Benjamin T., and Akhmanova, Anna

Abstract

The cross-talk between dynamic microtubules and integrin-based adhesions to the extracellular matrix plays a crucial role in cell polarity and migration. Microtubules regulate the turnover of adhesion sites, and, in turn, focal adhesions promote cortical microtubule capture and stabilization in their vicinity, but the underlying mechanism is unknown. Here, we show that cortical microtubule stabilization sites containing CLASPs, KIF21A, LL5? and liprins are recruited to focal adhesions by the adaptor protein KANK1, which directly interacts with the major adhesion component, talin. Structural studies showed that the conserved KN domain in KANK1 binds to the talin rod domain R7. Perturbation of this interaction, including a single point mutation in talin, which disrupts KANK1 binding but not the talin function in adhesion, abrogates the association of microtubule-stabilizing complexes with focal adhesions. We propose that the talin-KANK1 interaction links the two macromolecular assemblies that control cortical attachment of actin fibers and microtubules.

Published
2016

24. Author response: Talin-KANK1 interaction controls the recruitment of cortical microtubule stabilizing complexes to focal adhesions

Author

Bouchet, Benjamin P, primary, Gough, Rosemarie E, additional, Ammon, York-Christoph, additional, van de Willige, Dieudonnée, additional, Post, Harm, additional, Jacquemet, Guillaume, additional, Altelaar, AF Maarten, additional, Heck, Albert JR, additional, Goult, Benjamin T, additional, and Akhmanova, Anna, additional

Published
2016
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26. SCARECROW-LIKE23 and SCARECROW jointly specify endodermal cell fate but distinctly control SHORT-ROOT movement

Author

Dep Biologie, Sub Cell Biology, Celbiologie, Long, Yuchen, Goedhart, Joachim, Schneijderberg, Martinus, Terpestra, Inez, Shimotohno, Akie, Bouchet, Benjamin P, Akhmanova, Anna, Gadella, Theodorus W J, Heidstra, Renze, Scheres, Ben, Blilou, Ikram, Dep Biologie, Sub Cell Biology, Celbiologie, Long, Yuchen, Goedhart, Joachim, Schneijderberg, Martinus, Terpestra, Inez, Shimotohno, Akie, Bouchet, Benjamin P, Akhmanova, Anna, Gadella, Theodorus W J, Heidstra, Renze, Scheres, Ben, and Blilou, Ikram

Published
2015

27. Arabidopsis BIRD Zinc Finger Proteins Jointly Stabilize Tissue Boundaries by Confining the Cell Fate Regulator SHORT-ROOT and Contributing to Fate Specification

Author

Dep Biologie, Celbiologie, Sub Cell Biology, Long, Yuchen, Smet, Wouter, Cruz-Ramírez, Alfredo, Castelijns, Bas, de Jonge, Wim, Mähönen, Ari Pekka, Bouchet, Benjamin P, Perez, Gabino Sanchez, Akhmanova, Anna, Scheres, Ben, Blilou, Ikram, Dep Biologie, Celbiologie, Sub Cell Biology, Long, Yuchen, Smet, Wouter, Cruz-Ramírez, Alfredo, Castelijns, Bas, de Jonge, Wim, Mähönen, Ari Pekka, Bouchet, Benjamin P, Perez, Gabino Sanchez, Akhmanova, Anna, Scheres, Ben, and Blilou, Ikram

Published
2015

28. Rb and FZR1/Cdh1 determine CDK4/6-cyclin D requirement in C. elegans and human cancer cells

Author

Developmental Biology, Celbiologie, Biomolecular Mass Spectrometry and Proteomics, Sub Developmental Biology, Sub Cell Biology, Sub Biomol.Mass Spectrometry & Proteom., Sub Biomol.Mass Spect. and Proteomics, Sub Inorganic Chemistry and Catalysis, The, Inge, Ruijtenberg, Suzan, Bouchet, Benjamin P, Cristobal Gonzalez de Durana, Alba, Prinsen, Martine B W, van Mourik, Tim, Koreth, John, Xu, Huihong, Heck, Albert J R, Akhmanova, Anna, Cuppen, Edwin, Boxem, Mike, Munoz Murillo, Ara, van den Heuvel, Sander, Developmental Biology, Celbiologie, Biomolecular Mass Spectrometry and Proteomics, Sub Developmental Biology, Sub Cell Biology, Sub Biomol.Mass Spectrometry & Proteom., Sub Biomol.Mass Spect. and Proteomics, Sub Inorganic Chemistry and Catalysis, The, Inge, Ruijtenberg, Suzan, Bouchet, Benjamin P, Cristobal Gonzalez de Durana, Alba, Prinsen, Martine B W, van Mourik, Tim, Koreth, John, Xu, Huihong, Heck, Albert J R, Akhmanova, Anna, Cuppen, Edwin, Boxem, Mike, Munoz Murillo, Ara, and van den Heuvel, Sander

Published
2015

29. Rb and FZR1/Cdh1 determine CDK4/6-cyclin D requirement in C. elegans and human cancer cells

Author

Hubrecht Institute with UMC, CMM Sectie Genomics and Bioinformatics, Cancer, Child Health, The, Inge, Ruijtenberg, Suzan, Bouchet, Benjamin P., Cristobal, Alba, Prinsen, Martine B W, Van Mourik, Tim, Koreth, John, Xu, Huihong, Heck, Albert J R, Akhmanova, Anna, Cuppen, Edwin, Boxem, Mike, Muñoz, Javier, Van Den Heuvel, Sander, Hubrecht Institute with UMC, CMM Sectie Genomics and Bioinformatics, Cancer, Child Health, The, Inge, Ruijtenberg, Suzan, Bouchet, Benjamin P., Cristobal, Alba, Prinsen, Martine B W, Van Mourik, Tim, Koreth, John, Xu, Huihong, Heck, Albert J R, Akhmanova, Anna, Cuppen, Edwin, Boxem, Mike, Muñoz, Javier, and Van Den Heuvel, Sander

Published
2015

30. CFEOM1-Associated Kinesin KIF21A Is a Cortical Microtubule Growth Inhibitor

Author

van der Vaart, Babet, primary, van Riel, Wilhelmina E., additional, Doodhi, Harinath, additional, Kevenaar, Josta T., additional, Katrukha, Eugene A., additional, Gumy, Laura, additional, Bouchet, Benjamin P., additional, Grigoriev, Ilya, additional, Spangler, Samantha A., additional, Yu, Ka Lou, additional, Wulf, Phebe S., additional, Wu, Jingchao, additional, Lansbergen, Gideon, additional, van Battum, Eljo Y., additional, Pasterkamp, R. Jeroen, additional, Mimori-Kiyosue, Yuko, additional, Demmers, Jeroen, additional, Olieric, Natacha, additional, Maly, Ivan V., additional, Hoogenraad, Casper C., additional, and Akhmanova, Anna, additional

Published
2013
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32. Microtubule Plus-End Tracking Proteins SLAIN 1/2 and ch-TOG Promote Axonal Development.

Author

van der Vaart, Babet, Franker, Mariella A. M., Kuijper, Marijn, Shasha Hua, Bouchet, Benjamin P., Kai Jiang, Grigoriev, Ilya, Hoogenraad, Casper C., and Akhmanova, Anna

Subjects
MICROTUBULES, NEUROPLASTICITY, AXONS, NEURON development, GENE expression, PROMOTERS (Genetics), PROTEIN-protein interactions
Abstract

Development, polarization, structural integrity, and plasticity of neuronal cells critically depend on the microtubule network and its dynamic properties. SLAIN1 and SLAIN2 are microtubule plus-end tracking proteins that have been recently identified as regulators of microtubule dynamics. SLAINs are targeted to microtubule tips through an interaction with the core components of microtubule plus-end tracking protein network, End Binding family members. SLAINs promote persistent microtubule growth by recruiting the microtubule polymerase ch-TOG to microtubule plus-ends. Here, we show that SLAIN1/2 and ch-TOG-proteins are highly enriched in brain and are expressed throughout mouse brain development. Disruption of the SLAIN-ch-TOG complex in cultured primary rat hippocampal neurons by RNA interference-mediated knockdown and a dominant-negative approach perturbs microtubule growth by increasing catastrophe frequency and inhibits axon extension during neuronal development. Our study shows that proper control of microtubule dynamics is important for axon elongation in developing neurons. [ABSTRACT FROM AUTHOR]

Published
2012
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33. Mesenchymal Cell Invasion Requires Cooperative Regulation of Persistent Microtubule Growth by SLAIN2 and CLASP1

Author

Bouchet, Benjamin P, Noordstra, Ivar, van Amersfoort, Miranda, Katrukha, Eugene A, Ammon, York-Christoph, Ter Hoeve, Natalie D, Hodgson, Louis, Dogterom, Marileen, Derksen, Patrick W B, Akhmanova, Anna, Celbiologie, Sub Cell Biology, Celbiologie, and Sub Cell Biology

Subjects
0301 basic medicine, rho GTP-Binding Proteins, cell migration, Microtubules, Polymerization, Mesoderm, Mice, CLIP-170, Rab6, Pseudopodia, 3D matrix, Rho GTPase, Cell migration, Cell biology, EB1, Female, Collagen, Microtubule-Associated Proteins, Microtubule-associated protein, Biology, Models, Biological, General Biochemistry, Genetics and Molecular Biology, Exocytosis, Article, Focal adhesion, 03 medical and health sciences, CLASP1, Microtubule, Cell Line, Tumor, Cell Adhesion, Journal Article, Animals, Humans, Neoplasm Invasiveness, ch-TOG, Cell adhesion, Molecular Biology, Interphase, Focal Adhesions, Cell morphogenesis, Cell Membrane, +TIPs, modeling, Cell Biology, tumor invasion, 030104 developmental biology, HEK293 Cells, Developmental Biology
Abstract

Microtubules regulate signaling, trafficking, and cell mechanics, but the respective contribution of these functions to cell morphogenesis and migration in 3D matrices is unclear. Here, we report that the microtubule plus-end tracking protein (+TIP) SLAIN2, which suppresses catastrophes, is not required for 2D cell migration but is essential for mesenchymal cell invasion in 3D culture and in a mouse cancer model. We show that SLAIN2 inactivation does not affect Rho GTPase activity, trafficking, and focal adhesion formation. However, SLAIN2-dependent catastrophe inhibition determines microtubule resistance to compression and pseudopod elongation. Another +TIP, CLASP1, is also needed to form invasive pseudopods because it prevents catastrophes specifically at their tips. When microtubule growth persistence is reduced, inhibition of depolymerization is sufficient for pseudopod maintenance but not remodeling. We propose that catastrophe inhibition by SLAIN2 and CLASP1 supports mesenchymal cell shape in soft 3D matrices by enabling microtubules to perform a load-bearing function.

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34. Induction of EMT by Twist Proteins as a Collateral Effect of Tumor-Promoting Inactivation of Premature Senescence

Author

Frédérique Fauvet, Agnès Doreau, Thibault Voeltzel, Abdelkader Selmi, Claude Caron de Fromentel, Benjamin Pierre Bouchet, Stéphane Ansieau, Alain Puisieux, Roberta Maestro, Claudio Doglioni, Clémence Thomas, Isabelle Puisieux, Sara Piccinin, Jérémy Bastid, Anne Pierre Morel, Sandrine Valsesia-Wittmann, Ansieau, Stephane, Bastid, Jeremy, Doreau, Agne, Morel Anne, Pierre, Bouchet Benjamin, P., Thomas, Clemence, Fauvet, Frederique, Puisieux, Isabelle, Doglioni, Claudio, Piccinin, Sara, Maestro, Roberta, Voeltzel, Thibault, Selmi, Abdelkader, Valsesia Wittmann, Sandrine, de Fromentel Claude, Caron, and Puisieux, Alain

Subjects
Cancer Research, animal structures, Transplantation, Heterologous, HUMDISEASE, Mice, Nude, Mice, Transgenic, Biology, Transfection, Retinoblastoma Protein, Cell Line, Mice, Twist transcription factor, Dogs, Downregulation and upregulation, Neoplasms, Animals, Humans, Neoplasm Invasiveness, Mammary Glands, Human, Cellular Senescence, Regulation of gene expression, Twist-Related Protein 1, Nuclear Proteins, Epithelial Cells, Cell Biology, Fibroblasts, Embryonic stem cell, Up-Regulation, Cell biology, Enzyme Activation, Gene Expression Regulation, Neoplastic, Mice, Inbred C57BL, Repressor Proteins, Transplantation, Cell Transformation, Neoplastic, Oncology, Mice, Inbred DBA, SIGNALING, Cell culture, Cell Transdifferentiation, Cancer cell, Immunology, ras Proteins, RNA Interference, CELLBIO, Tumor Suppressor Protein p53
Abstract

SummaryTwist1 and Twist2 are major regulators of embryogenesis. Twist1 has been shown to favor the metastatic dissemination of cancer cells through its ability to induce an epithelial-mesenchymal transition (EMT). Here, we show that a large fraction of human cancers overexpress Twist1 and/or Twist2. Both proteins override oncogene-induced premature senescence by abrogating key regulators of the p53- and Rb-dependent pathways. Twist1 and Twist2 cooperate with Ras to transform mouse embryonic fibroblasts. Interestingly, in epithelial cells, the oncogenic cooperation between Twist proteins and activated mitogenic oncoproteins, such as Ras or ErbB2, leads to complete EMT. These findings suggest an unanticipated direct link between early escape from failsafe programs and the acquisition of invasive features by cancer cells.

Published
2008

35. Advances and Challenges in Cell Biology for Cultured Meat.

Author

Martins B, Bister A, Dohmen RGJ, Gouveia MA, Hueber R, Melzener L, Messmer T, Papadopoulos J, Pimenta J, Raina D, Schaeken L, Shirley S, Bouchet BP, and Flack JE

Subjects
Animals, Animal Welfare, Cell Culture Techniques veterinary, Cell Differentiation, In Vitro Meat, Meat
Abstract

Cultured meat is an emerging biotechnology that aims to produce meat from animal cell culture, rather than from the raising and slaughtering of livestock, on environmental and animal welfare grounds. The detailed understanding and accurate manipulation of cell biology are critical to the design of cultured meat bioprocesses. Recent years have seen significant interest in this field, with numerous scientific and commercial breakthroughs. Nevertheless, these technologies remain at a nascent stage, and myriad challenges remain, spanning the entire bioprocess. From a cell biological perspective, these include the identification of suitable starting cell types, tuning of proliferation and differentiation conditions, and optimization of cell-biomaterial interactions to create nutritious, enticing foods. Here, we discuss the key advances and outstanding challenges in cultured meat, with a particular focus on cell biology, and argue that solving the remaining bottlenecks in a cost-effective, scalable fashion will require coordinated, concerted scientific efforts. Success will also require solutions to nonscientific challenges, including regulatory approval, consumer acceptance, and market feasibility. However, if these can be overcome, cultured meat technologies can revolutionize our approach to food.

Published
2024
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36. Microtubules in 3D cell motility.

Author

Bouchet BP and Akhmanova A

Subjects
Animals, Cellular Microenvironment, Extracellular Matrix metabolism, Humans, Morphogenesis, Cell Movement, Imaging, Three-Dimensional, Microtubules metabolism
Abstract

Three-dimensional (3D) cell motility underlies essential processes, such as embryonic development, tissue repair and immune surveillance, and is involved in cancer progression. Although the cytoskeleton is a well-studied regulator of cell migration, most of what we know about its functions originates from studies conducted in two-dimensional (2D) cultures. This research established that the microtubule network mediates polarized trafficking and signaling that are crucial for cell shape and movement in 2D. In parallel, developments in light microscopy and 3D cell culture systems progressively allowed to investigate cytoskeletal functions in more physiologically relevant settings. Interestingly, several studies have demonstrated that microtubule involvement in cell morphogenesis and motility can differ in 2D and 3D environments. In this Commentary, we discuss these differences and their relevance for the understanding the role of microtubules in cell migration in vivo We also provide an overview of microtubule functions that were shown to control cell shape and motility in 3D matrices and discuss how they can be investigated further by using physiologically relevant models., (© 2017. Published by The Company of Biologists Ltd.)

Published
2017
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37. Microtubule plus-end tracking proteins SLAIN1/2 and ch-TOG promote axonal development.

Author

van der Vaart B, Franker MA, Kuijpers M, Hua S, Bouchet BP, Jiang K, Grigoriev I, Hoogenraad CC, and Akhmanova A

Subjects
Amino Acid Sequence, Animals, Animals, Newborn, Cells, Cultured, Female, Hippocampus embryology, Humans, Male, Mice, Molecular Sequence Data, Rats, Axons physiology, Hippocampus physiology, Microtubule-Associated Proteins physiology, Proteins physiology
Abstract

Development, polarization, structural integrity, and plasticity of neuronal cells critically depend on the microtubule network and its dynamic properties. SLAIN1 and SLAIN2 are microtubule plus-end tracking proteins that have been recently identified as regulators of microtubule dynamics. SLAINs are targeted to microtubule tips through an interaction with the core components of microtubule plus-end tracking protein network, End Binding family members. SLAINs promote persistent microtubule growth by recruiting the microtubule polymerase ch-TOG to microtubule plus-ends. Here, we show that SLAIN1/2 and ch-TOG-proteins are highly enriched in brain and are expressed throughout mouse brain development. Disruption of the SLAIN-ch-TOG complex in cultured primary rat hippocampal neurons by RNA interference-mediated knockdown and a dominant-negative approach perturbs microtubule growth by increasing catastrophe frequency and inhibits axon extension during neuronal development. Our study shows that proper control of microtubule dynamics is important for axon elongation in developing neurons.

Published
2012
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