88 results on '"Bouaoud J"'
Search Results
2. Integra® dermal regeneration template for full thickness carcinologic scalp defects: Our 6 years’ experience retrospective cohort and literature review
- Author
-
Romano, G., Bouaoud, J., Moya-Plana, A., Benmoussa, N., Honart, J.-F., and Leymarie, N.
- Published
- 2021
- Full Text
- View/download PDF
3. Use of injectables in rhinoplasty retouching: Towards an evolution of surgical strategy? Literature review
- Author
-
Bouaoud, J. and Belloc, J.-B.
- Published
- 2020
- Full Text
- View/download PDF
4. Reduction of morbidity of the revascularization surgery in the management of mandibular osteoradionecrosis by basilar edge preservation
- Author
-
Bettoni, J., Bouaoud, J., Duisit, J., Dakpé, S., Olivetto, M., and Devauchelle, B.
- Published
- 2020
- Full Text
- View/download PDF
5. Ferite cervicali penetranti
- Author
-
Caruhel, J.-B., Bouaoud, J., Tabchouri, N., Schouman, T., and Goudot, P.
- Published
- 2020
- Full Text
- View/download PDF
6. The 4-NQO mouse model: An update on a well-established in vivo model of oral carcinogenesis
- Author
-
Bouaoud, J., primary, De Souza, G., additional, Darido, C., additional, Tortereau, A., additional, Elkabets, M., additional, Bertolus, C., additional, and Saintigny, P., additional
- Published
- 2021
- Full Text
- View/download PDF
7. Severe macroglossia after posterior fossa and craniofacial surgery in children
- Author
-
Bouaoud, J., Joly, A., Picard, A., Thierry, B., Arnaud, E., James, S., Hennessy, I., McGarvey, B., Cairet, P., Vecchione, A., Vergnaud, E., Duracher, C., and Khonsari, R.H.
- Published
- 2018
- Full Text
- View/download PDF
8. Caudal vertebra rat free flap in the groin: A preliminary study of a new combined osteocutaneous flap model
- Author
-
Sounthakith, V., Neiva-Vaz, C., Picard, A., Vignes, J-L., Bouaoud, J., and Fitoussi, F.
- Published
- 2024
- Full Text
- View/download PDF
9. Protuberant fibro-osseous lesion of the temporal bone: report of four cases and review of the literature
- Author
-
Bouaoud, J., primary, Larousserie, F., additional, Galmiche-Rolland, L., additional, Bouvier, C., additional, Picard, A., additional, and Khonsari, R.H., additional
- Published
- 2021
- Full Text
- View/download PDF
10. Challenges in lower face soft tissue reconstruction: The value of the historical bipedicled scalp flap procedure
- Author
-
Olivetto, M., Bettoni, J., Duisit, J., Dakpé, S., Testelin, S., Bouaoud, J., and Devauchelle, B.
- Published
- 2021
- Full Text
- View/download PDF
11. Virtual peer role-play during COVID-19 pandemic for teaching medical students how to break bad news
- Author
-
Bouaoud J and Saintigny P
- Subjects
Response rate (survey) ,Medical education ,Coronavirus disease 2019 (COVID-19) ,business.industry ,media_common.quotation_subject ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Order (business) ,Pandemic ,Health care ,ComputingMilieux_COMPUTERSANDEDUCATION ,Quality (business) ,Session (computer science) ,Psychology ,business ,media_common - Abstract
In order to cope with the SARS-CoV-2 pandemic and meet with the educational needs of medical students, we have evaluated the virtual peer role-plays (VPRP), an innovative approach to teach breaking bad news communication skills to medical students. Three scenarios of relational simulation were successively proposed to 237 medical students divided in 10 groups, each supervised by two teachers. Pre- and post-VPRP questionnaires were submitted to evaluate students’ satisfaction. The response rate of the pre- and post-VPRP questionnaires were 89% and 52% respectively. Two-thirds of the students had never participated in a peer role-play session. Most students had low level of confidence in breaking bad news communication and were motivated to participate to the VPRP session. Students’ satisfaction on VPRP session regarding quality (realism, organization), interest, perceived benefits was very positive. In conclusion, VPRP are feasible, of low cost (no material is required), applicable to other healthcare students and is relevant to the growth of teleconsultation.
- Published
- 2021
12. Authors responses on the comments regarding our article “Use of injectables in rhinoplasty retouching: towards an evolution of surgical strategy? Literature review”
- Author
-
Bouaoud, J. and Belloc, J.-B.
- Published
- 2020
- Full Text
- View/download PDF
13. How to manage calcified vessels for head and neck microsurgical reconstruction
- Author
-
Bouaoud, J., Honart, J.-F., Bennis, Y., and Leymarie, N.
- Published
- 2020
- Full Text
- View/download PDF
14. Locally advanced nasal pyramid squamous cell carcinoma: our 15 years’ experience in a series of 35 total rhinectomies
- Author
-
Antoine Moya-Plana, Hennocq Q, Classe M, Nicolas Leymarie, Philippe Gorphe, F Kolb, Bouaoud J, Pierre Blanchard, Ingrid Breuskin, Stéphane Temam, N. Benmoussa, François Janot, Jean-François Honart, and Odile Casiraghi
- Subjects
Series (stratigraphy) ,medicine.medical_specialty ,business.industry ,Locally advanced ,General Medicine ,Nose ,medicine.disease ,Otorhinolaryngology ,Pyramid ,Carcinoma, Squamous Cell ,Carcinoma ,medicine ,Humans ,Basal cell ,Radiology ,business - Published
- 2020
15. Reduction of morbidity of the revascularization surgery in the management of mandibular osteoradionecrosis by basilar edge preservation.
- Author
-
UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - (SLuc) Service de chirurgie plastique, Bettoni, J, Bouaoud, J, Duisit, Jérôme, Dakpé, S, Olivetto, M, Devauchelle, B, UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - (SLuc) Service de chirurgie plastique, Bettoni, J, Bouaoud, J, Duisit, Jérôme, Dakpé, S, Olivetto, M, and Devauchelle, B
- Abstract
The chronic complications of bone free-flap revascularization surgery are mainly characterized by skin fistulization of the osteosynthesis material, which sometimes leads to reactivation of the osteoradionecrotic phenomenon. The objective of the study is to evaluate the benefit of mandibular basilar edge preservation in bone reconstructive surgery in irradiated areas performed for the treatment of advanced mandibular osteoradionecrosis. A retrospective monocentric study conducted between 2003 and 2018 including all patients undergoing revascularization surgery for the treatment of advanced osteoradionecrotic lesion with respect to the basilar margin was conducted. Eight patients (7 males and 1 female, aged 50 to 63 years) who had a marginal mandibulectomy with reconstruction by bone free flap or composite free flap were included. The stability of the reconstruction (junction native mandible/bone free flap) was achieved on average by using 1.75 [range 0-4] mini plates (Medartis® Modus 2.0, Medartis AG, Basel, Switzerland). During the follow-up (30±13 months) no chronic complication related to a dissociation of the osteosynthesis material or a reactivation of the osteoradionecrotic phenomenon were identified. Despite the limited number of patients, it seems that the increase in the friction surface between the free flap and the native mandibular bone, linked to the preservation of the basilar edge, improves the primary stability of the reconstruction. This reduction in mechanical stress on osteosynthesis materials limits its use and reduces the rate of chronic complications of bone flap revascularization surgery in irradiated areas.
- Published
- 2020
16. Technical note on the harvest of periosteal forearm composite free flaps in the treatment of early mandibular osteoradionecrotic injury
- Author
-
Bettoni, J., Olivetto, M., Bouaoud, J., and Devauchelle, B.
- Published
- 2019
- Full Text
- View/download PDF
17. Muscle entrapment in orbito-zygomatico-maxillary complex fracture
- Author
-
Bouaoud, J., Olivetto, M., Bettoni, J., and Devauchelle, B.
- Published
- 2019
- Full Text
- View/download PDF
18. Locally advanced nasal pyramid squamous cell carcinoma: our 15 years’ experience in a series of 35 total rhinectomies
- Author
-
Bouaoud, J., primary, Benmoussa, N., additional, Hennocq, Q., additional, Honart, J.-F., additional, Breuskin, I., additional, Gorphe, P., additional, Casiraghi, O., additional, Classe, M., additional, Blanchard, P., additional, Janot, F., additional, Kolb, F., additional, Leymarie, L., additional, Temam, S., additional, and Moya-Plana, A., additional
- Published
- 2020
- Full Text
- View/download PDF
19. Fraser syndrome: review of the literature illustrated by a historical adult case
- Author
-
Bouaoud, J., primary, Olivetto, M., additional, Testelin, S., additional, Dakpe, S., additional, Bettoni, J., additional, and Devauchelle, B., additional
- Published
- 2020
- Full Text
- View/download PDF
20. An asymptomatic palatal tumor
- Author
-
Olivetto, M., primary, Bouaoud, J., additional, and Bettoni, J., additional
- Published
- 2020
- Full Text
- View/download PDF
21. S14-02 SESSION 14
- Author
-
Bouaoud, J., primary, Khonsari, R. H., additional, James, S., additional, Paternoster, G., additional, and Arnaud, E., additional
- Published
- 2019
- Full Text
- View/download PDF
22. Familial lumps of the lower jaw
- Author
-
Bouaoud, J., Picard, A., Joly, A., and Khonsari, R.H.
- Published
- 2017
- Full Text
- View/download PDF
23. Minimum follow-up for closed rhinoseptoplasties.
- Author
-
Belloc JB, Mure C, Mathieu O, and Bouaoud J
- Subjects
- Humans, Female, Male, Follow-Up Studies, Adult, Prospective Studies, Middle Aged, Treatment Outcome, Esthetics, Rhinoplasty methods, Nasal Septum surgery, Nasal Septum pathology, Nasal Obstruction surgery, Nasal Obstruction diagnosis
- Abstract
Background: There is no established consensus on the postoperative follow-up from which the aesthetic and functional outcomes of rhinoseptoplasty are considered as stable., Objectives: To contribute to defining the postoperative follow-up from which rhinoseptoplasty outcomes cease to evolve., Methods: Postoperative assessments of Nasal Obstruction Symptom Evaluation (NOSE) and Rhinoplasty Outcome Evaluation (ROE) scores from 357 closed structural rhinoseptoplasty procedures were prospectively gathered from January 2019 to December 2023. These measurements encompassed the postoperative period from 1 to 12 months. All procedures were performed utilizing closed technique., Results: No statistically significant difference was detected between the scores at 1, 2, and 6 months versus 12 months postoperatively (ROE: p = 0.388; 0.268; 0.162; NOSE: p = 0.265; 0.192; 0.975, Mann-Whitney test). Similarly, no follow-up impact was revealed between the scores at 1, 2, 6, and 12 months postoperatively (ROE: p = 0.548; NOSE: p = 0.280, Kruskal-Wallis test). No significant correlation was established between follow-up (in months) and ROE and NOSE scores (ROE: p = 0.397; NOSE: p = 0.632, Spearman)., Conclusion: Follow-up duration does not influence NOSE and ROE scores over the 1- to 12-month timeframe. The 1-month postoperative outcome can be regarded as a reliable indicator of the 12-month outcome. These conclusions apply to NOSE and ROE scores of rhinoseptoplasty conducted using closed technique for the 1- to 12-month period. Further research is needed for open techniques, preservation rhinoplasty, other patient-reported outcomes measures (PROMs) as well as for the follow-up beyond 12 months postoperatively., Competing Interests: Declaration of competing interest The authors have no conflict of interest to declare in relation to the content of this article., (Copyright © 2024 Elsevier Masson SAS. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
24. Extensive head and neck skin cancers: Carcinologic surgery as a cornerstone of treatment.
- Author
-
Bondi T, Chaine A, Foy JP, Benassarou M, Bertolus C, and Bouaoud J
- Subjects
- Humans, Male, Female, Aged, Retrospective Studies, Aged, 80 and over, Middle Aged, Adult, Plastic Surgery Procedures methods, Plastic Surgery Procedures statistics & numerical data, Skin Neoplasms surgery, Skin Neoplasms pathology, Skin Neoplasms epidemiology, Skin Neoplasms therapy, Skin Neoplasms diagnosis, Head and Neck Neoplasms surgery, Head and Neck Neoplasms pathology, Head and Neck Neoplasms therapy, Head and Neck Neoplasms epidemiology, Head and Neck Neoplasms diagnosis, Carcinoma, Squamous Cell surgery, Carcinoma, Squamous Cell pathology, Carcinoma, Squamous Cell therapy, Carcinoma, Squamous Cell diagnosis, Carcinoma, Squamous Cell epidemiology, Carcinoma, Basal Cell surgery, Carcinoma, Basal Cell pathology, Carcinoma, Basal Cell epidemiology
- Abstract
Background and Objective: The prevalence of extensive skin cancers increases with the aging of the population. Surgical management is the gold standard of curative treatment while morbidity is not negligible. There are few data in the literature concerning extensive head and neck cutaneous cancers. The aim of this article is to report our experience of curative management of head and neck extensive skin cancers., Method: In this single-center retrospective observational study, we report a series of 17 patients with extensive skin facial cancers treated by surgery between 2013 and 2022 in the maxillofacial surgery department of the Pitié-Salpêtrière Hospital. We collected clinical, therapeutic, histological, and carcinologic data., Results: The median age of the patients was 66 years [35-94]. There were 9 male and 8 women. Scalp (39 %) and cheek (22 %) locations were the most frequent ones. The most frequent histological types were squamous cell carcinoma (61 %) and basal cell carcinoma (17 %). Three patients received neoadjuvant treatment. The surgical treatment consisted mainly of carcinological resection followed by one-stage reconstruction by free flap for 5 (30 %) patients and without reconstruction for primary for 12 (70 %) patients, of whom 8 benefited from secondary reconstruction. Five patients received adjuvant radiotherapy or radio-chemotherapy. With a median follow-up of 40 months (2-72), the median overall survival was 40 months (12-72)., Conclusion: We know that extensive skin cancers of the face have a good prognosis on condition that the carcinological and reconstructive requirements are respected. Surgery remains the cornerstone of treatment while the improvement of adjuvant therapies, in particular the rise of immunotherapies or other targeted therapies, may allow to limit recurrences., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Masson SAS. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
25. Epidemiology of facial skin cancers managed in a French ambulatory surgical center.
- Author
-
Mathieu O, Chaine A, Benassarou M, Combes F, Debelmas A, Lanciaux S, Bertolus C, and Bouaoud J
- Subjects
- Humans, Male, Female, Aged, Retrospective Studies, Middle Aged, France epidemiology, Aged, 80 and over, Adult, Carcinoma, Squamous Cell epidemiology, Carcinoma, Squamous Cell pathology, Carcinoma, Squamous Cell surgery, Carcinoma, Squamous Cell diagnosis, Ambulatory Surgical Procedures statistics & numerical data, Tertiary Care Centers statistics & numerical data, Skin Neoplasms epidemiology, Skin Neoplasms surgery, Skin Neoplasms pathology, Skin Neoplasms diagnosis, Facial Neoplasms epidemiology, Facial Neoplasms surgery, Facial Neoplasms pathology, Facial Neoplasms diagnosis, Carcinoma, Basal Cell epidemiology, Carcinoma, Basal Cell surgery, Carcinoma, Basal Cell pathology, Carcinoma, Basal Cell diagnosis
- Abstract
Introduction: Approximately 80,000 cases of skin cancer are diagnosed annually in France. The management of these cancers can occur in both university hospital centers and ambulatory surgery centers. Limited data exist regarding the epidemiology of cutaneous cancers treated through ambulatory surgery centers. The objective of our study is to describe the epidemiological characteristics of cutaneous cancers managed in a tertiary ambulatory surgery center., Methods: This is a retrospective, single-center observational study. The included patients were those who underwent surgical excision of one or more skin cancers within the maxillofacial department of a tertiary ambulatory surgery center. Clinical, therapeutic, histopathological, and follow-up data (additional surgery if margins were not clear, progression, recurrence, second cancer…) were collected., Results: Among the n = 1931 patients operated for a head and neck skin tumor from September 2018 to July 2022, n = 426 (22 %) were diagnosed with cancer upon histological analysis. The median age was 76 years (31-100), with a male-to-female ratio of 1/1. The most frequent locations were the nose (23 %) and cheek (20 %). Ten percent of patients had dual-site skin cancer at initial diagnosis. The most common histological types were basal cell carcinoma (77 %) and squamous cell carcinoma (18 %). Surgical treatment primarily consisted of "excision-reconstruction with local flap" (51 %) or "excision-suture" (34 %). Resection margins were mostly clear (65 %), and only six patients (2 %) experienced local recurrence or progression during follow-up., Conclusions: Skin cancers are prevalent in ambulatory practice. Surgical treatment allows for effective control of the cancer. Photoprotection, particularly in immunocompromised patients, remains crucial for prevention., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this article., (Copyright © 2023 Elsevier Masson SAS. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
26. From precise surgery to precision surgery: The multiple dimensions of therapeutic precision for head and neck cancer.
- Author
-
Galmiche A, Saidak Z, Babin E, Brenet E, Davrou J, Fournier I, Devauchelle B, Testelin S, Dakpe S, Pellet A, Thariat J, Bastit V, Clatot F, Saintigny P, Bouaoud J, and Foy JP
- Subjects
- Humans, Head and Neck Neoplasms diagnosis, Head and Neck Neoplasms surgery, Carcinoma, Squamous Cell
- Published
- 2023
- Full Text
- View/download PDF
27. Biomarkers of radioresistance in head and neck squamous cell carcinomas.
- Author
-
Avril D, Foy JP, Bouaoud J, Grégoire V, and Saintigny P
- Subjects
- Humans, Biomarkers, Squamous Cell Carcinoma of Head and Neck radiotherapy, Carcinoma, Squamous Cell radiotherapy, Head and Neck Neoplasms radiotherapy, Papillomavirus Infections complications
- Abstract
Purpose: Head and neck squamous cell carcinoma (HNSCC) is a major cause of morbidity and mortality. Although HNSCC is mainly caused by tobacco and alcohol consumption, infection by Human Papilloma Virus (HPV) has been also associated with the increasing incidence of oropharyngeal squamous cell carcinomas (OPSCC) during the past decades. HPV-positive HNSCC is characterized by a higher radiosensitivity compared to HPV-negative tumor. While several clinical trials are evaluating de-escaladed radiation doses strategies in HPV-positive HNSCC, molecular mechanisms associated with relative radioresistance in HPV-negative HNSCC are still broadly unknown. Our goal was to review recently proposed biomarkers of radioresistance in this setting, which may be useful for stratifying tumor's patient according to predicted level of radioresistance., Conclusions: most of biomarkers of radioresistance in HPV-negative HNSCC are identified using a hypothesis-driven approach, based on molecular mechanisms known to play a key role during carcinogenesis, compared to an unsupervised data-driven approach regardless the biological rational. DNA repair and hypoxia are the two most widely investigated biological and targetable pathways related to radioresistance in HNSCC. The better understanding of molecular mechanisms and biomarkers of radioresistance in HPV-negative HNSCC could help for the development of radiosensitization strategies, based on targetable biomarkers, in radioresistant tumors as well as de-escalation radiation dose strategies, based on biological level of radioresistance, in radiosensitive tumors.
- Published
- 2023
- Full Text
- View/download PDF
28. Immunologically active phenotype by gene expression profiling is associated with clinical benefit from PD-1/PD-L1 inhibitors in real-world head and neck and lung cancer patients.
- Author
-
Foy JP, Karabajakian A, Ortiz-Cuaran S, Boussageon M, Michon L, Bouaoud J, Fekiri D, Robert M, Baffert KA, Hervé G, Quilhot P, Attignon V, Girod A, Chaine A, Benassarou M, Zrounba P, Caux C, Ghiringhelli F, Lantuejoul S, Crozes C, Brochériou I, Pérol M, Fayette J, Bertolus C, and Saintigny P
- Subjects
- B7-H1 Antigen, Cetuximab therapeutic use, Gene Expression Profiling, Humans, Immune Checkpoint Inhibitors, Phenotype, Programmed Cell Death 1 Receptor therapeutic use, Squamous Cell Carcinoma of Head and Neck drug therapy, Squamous Cell Carcinoma of Head and Neck genetics, Carcinoma, Non-Small-Cell Lung drug therapy, Carcinoma, Non-Small-Cell Lung genetics, Carcinoma, Non-Small-Cell Lung pathology, Head and Neck Neoplasms drug therapy, Head and Neck Neoplasms genetics, Lung Neoplasms drug therapy, Lung Neoplasms genetics, Lung Neoplasms pathology
- Abstract
Introduction: Identification of tumours harbouring an overall active immune phenotype may help for selecting patients with advanced head and neck squamous cell carcinomas (HNSCC) and non-small cell lung cancer (NSCLC) who may benefit from immunotherapies. Our objective was to develop a reliable and stable scoring system to identify those immunologically active tumours., Methods: Using gene expression profiles of 421 HNSCC, we developed a score to identify immunologically active tumours. Validation of the 'HOT' score was done in 40 HNSCC and 992 NSCLC. Stability of the 'HOT' score was tested in paired HNSCC samples from diagnostic biopsies versus surgically resected specimens, untreated versus recurrent samples, and pre-versus post-cetuximab samples in a total of 76 patients. The association between the 'HOT' score with overall survival (OS) and progression-free survival (PFS) was tested in 184 patients with HNSCC or NSCLC treated with PD-1/PD-L1 inhibitors., Results: A 27-gene expression based 'HOT' score was correlated with: (i) PD-L1 and IDO1 expression, (ii) TCD8 infiltrate and (iii) activation of the IFN-γ pathway. The HOT score concordance when comparing diagnostic biopsies and surgically resected specimens was higher than in untreated samples versus recurrent or pre-versus post-cetuximab samples. In 102 and 82 patients with HNSCC or NSCLC treated with PD-1/PD-L1 inhibitors, the HOT score was associated with an improved OS and PFS in multivariate analysis., Conclusion: The 'HOT' score is a simple and robust approach to identify real-world patients with HNSCC and NSCLC immunologically active tumours who may benefit from PD-1/PD-L1 inhibitors., (Copyright © 2022 Elsevier Ltd. All rights reserved.)
- Published
- 2022
- Full Text
- View/download PDF
29. Vestibuloplasty for immediate dental implantation in fibular free flap for oral cancer patients undergoing mandibulectomy reconstruction: A technical note.
- Author
-
Bouaoud J, Descols P, De Kerangal QLG, Honart JF, Moya-Plana A, Temam S, Brau JJ, D'andrea G, and Benmoussa N
- Subjects
- Dental Implantation, Humans, Mandibular Osteotomy, Quality of Life, Vestibuloplasty, Dental Implants, Free Tissue Flaps, Mouth Neoplasms surgery, Plastic Surgery Procedures
- Abstract
Vestibuloplasty is fundamental to restore an oral vestibule for immediate dental implantation in fibular free flap (FFF) for oral cancer patients undergoing mandibulectomy reconstruction. Double surgical team including reconstructive head and neck surgeon and a dental surgeon is fundamental. The first step of the vestibuloplasty is to identify the skin perforator. The second step is to thin the FFF skin island as much as necessary to facilitate: i-the reinset into the gingivobuccal sulcus while creating enough space in the oral vestibule for the future dental prosthesis and ii-the exposition of dental implants. The third step is to create a percutaneous access to the implants through the FFF skin paddle using a dermatologic punch while preserving a large oral vestibule. The fourth step is the skin reinsertion into the gingivobuccal sulcus and closure. Realizing vestibuloplasty before radiotherapy allows prevention of soft tissue contraction and osteoradionecrosis while reducing the necessary time for a complete dental rehabilitation and improving patient quality of life., (Copyright © 2021 Elsevier Masson SAS. All rights reserved.)
- Published
- 2022
- Full Text
- View/download PDF
30. Datasets for gene expression profiles of head and neck squamous cell carcinoma and lung cancer treated or not by PD1/PD-L1 inhibitors.
- Author
-
Foy JP, Karabajakian A, Ortiz-Cuaran S, Boussageon M, Michon L, Bouaoud J, Fekiri D, Robert M, Baffert KA, Hervé G, Quilhot P, Attignon V, Girod A, Chaine A, Benassarou M, Zrounba P, Caux C, Ghiringhelli F, Lantuejoul S, Crozes C, Brochériou I, Pérol M, Fayette J, Bertolus C, and Saintigny P
- Abstract
Identification of tumors harboring an overall active immune phenotype may help for selecting patients with advanced head and neck squamous cell carcinomas (HNSCC) and non-small cell lung cancer (NSCLC) who may benefit from immunotherapies. In this context, we generated targeted gene expression profiles in three and two independent cohorts of patients with HNSCC or NSCLC respectively, treated or not by PD-1/PD-L1 inhibitors. Notably, we generated two datasets including 102 and 82 patients with HNSCC or NSCLC treated with PD-1/PD-L1 inhibitors. Clinical information, including detailed survival raw data, is available for each patient, allowing to test association between gene expression data and patient survival (overall and progression-free survival). Moreover, we also generated gene expression datasets of 27 paired HNSCC samples from diagnostic biopsies and versus surgically resected specimens as well as 33 paired HNSCC samples at initial diagnosis (untreated) and at recurrence. Those datasets may allow to test the stability of a given biomarker across paired samples., Competing Interests: The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: PS is a member of HTG Diagnostics Scientific Advisory Board and receives research grants from HTG Diagnostics, Inivata, ArcherDx, Bristol-Myers Squibb, Roche Molecular Diagnostics, Roche, AstraZeneca, Novartis, Bristol-Myers Squibb Foundation and Illumina; JF reports grants, personal fees and non-financial support from Bristol-Myers Squibb, personal fees and non-financial support from MSD, personal fees from Merck, personal fees and non-financial support from Astrazeneca, personal fees from Rakuten, personal fees from Biogen and personal fees from Innate Pharma; SL received advisory board honoraria from AbbVie, AstraZeneca, Bayer, Bristol-Myers Squibb, Merck Sharp and Dohme, Takeda, Roche/Genentech; FG reports personal grants consultancy for Roche, AstraZeneca, and payments for development of educational presentations for Roche, Servier, Amgen, Merck. All remaining authors have declared no conflicts of interest., (© 2022 The Authors.)
- Published
- 2022
- Full Text
- View/download PDF
31. Teaching how to break bad news in Oncology: In-class vs. virtual peer role-plays.
- Author
-
Bouaoud J, Michon L, and Saintigny P
- Subjects
- Clinical Competence, Communication, Humans, Peer Group, SARS-CoV-2, Truth Disclosure, COVID-19, Students, Medical
- Abstract
Background: We report two different peer role-play training courses for breaking bad news (BBN) in Oncology, the classic "in-class" model and the "virtual" peer role-play (VPRP) model developed during the SARS-CoV-2 pandemic., Methods: Each session included 20-25 4th year medical students supervised by two practitioners experienced in oncology. After an ice breaking activity to exchange with students on means to promote hope to patients when BBN, peer role-plays started. Pre-and post-session questionnaires were submitted to evaluate students' satisfaction, attitudes, and perceptions. Pre-and post-session knowledge test were realized. Each student has participated to only one peer-role play either "in-class" (2018) or VPRP (2020)., Results: In 2018, a total of 222 students received the "in-class" training. In 2020, a total 431 students received the VPRP training. For almost all students it was the first peer role-play training session. Before training, reported level of confidence in BBN was low. After training, students of the VPRP group were highly satisfied regarding quality (realism, organization). Students also reported great interest and perceived benefits. Students who underwent "in-class" training course showed a significantly higher improvement (+1.9 points) of their knowledge scores compared to those who underwent the VPRP training course (+0.7 points) (P-value=2e-16)., Conclusion: The two methods seem beneficial to improve knowledge skills in BBN although "in-class" training class seem to be more efficient. To our knowledge, this is the first comparison between virtual and in-class peer-role play training for BBN in oncology., (Copyright © 2022 Société Française du Cancer. Published by Elsevier Masson SAS. All rights reserved.)
- Published
- 2022
- Full Text
- View/download PDF
32. Head and neck Ewing sarcoma: French surgical practice analysis pleads for surgery centralization.
- Author
-
Bouaoud J, Temam S, Galmiche L, Cozic N, Bolle S, Belhous K, Kolb F, Qassemyar Q, Bidault F, Couloigner V, Picard A, Le Deley MC, Mahier-Ait Oukhatar C, Gaspar N, and Kadlub N
- Subjects
- Combined Modality Therapy, Esthetics, Dental, Humans, Margins of Excision, Neoplasm Recurrence, Local, Retrospective Studies, Head and Neck Neoplasms surgery, Neoplasms, Second Primary, Sarcoma, Ewing surgery
- Abstract
This study aimed to analyze surgical procedures for head and neck Ewing sarcoma (HNES) with regard to oncological, functional, and esthetic outcomes. A blinded multidisciplinary retrospective chart review of operated French HNES patients (Euro-EWING 99 trial, 1999-2014) was performed to assess patient/tumor characteristics, treatment details, and outcomes. Primary surgery without reconstruction was undertaken in 13 patients (emergency context/misdiagnosis). However, because of contaminated surgical margins, all patients had to undergo systematic postoperative radiotherapy. Twenty-six patients underwent multidisciplinary evaluation and were scheduled to undergo postchemotherapy surgery, with 19 patients scheduled for immediate reconstruction. All cases showed R0 margins after postchemotherapy surgery of the initial tumor bed by multidisciplinary surgical teams, while n = 3/4 of local relapses (very poor prognosis) had R1a margins after surgery of the residual tumor volume following chemotherapy. Only three surgical expertise centers operated on ≥ 4 patients over the 15-year period. Thirty patients developed long-term sequelae, with increased complications following radiotherapy. Referring patients to surgical expertise centers following a suspected diagnosis, with planned postchemotherapy surgery of the initial tumor bed at these centers, might limit the need for intralesional resections, allowing radical R0 resections and thus reducing long-term sequelae as well as the risk of secondary radio-induced malignancy by limiting the need for postoperative radiotherapy., (Copyright © 2021 European Association for Cranio-Maxillo-Facial Surgery. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2022
- Full Text
- View/download PDF
33. Unmet Needs and Perspectives in Oral Cancer Prevention.
- Author
-
Bouaoud J, Bossi P, Elkabets M, Schmitz S, van Kempen LC, Martinez P, Jagadeeshan S, Breuskin I, Puppels GJ, Hoffmann C, Hunter KD, Simon C, Machiels JP, Grégoire V, Bertolus C, Brakenhoff RH, Koljenović S, and Saintigny P
- Abstract
Oral potentially malignant disorders (OPMD) may precede oral squamous cell carcinoma (OSCC). Reported rates of malignant transformation of OPMD range from 3 to 50%. While some clinical, histological, and molecular factors have been associated with a high-risk OPMD, they are, to date, insufficiently accurate for treatment decision-making. Moreover, this range highlights differences in the clinical definition of OPMD, variation in follow-up periods, and molecular and biological heterogeneity of OPMD. Finally, while treatment of OPMD may improve outcome, standard therapy has been shown to be ineffective to prevent OSCC development in patients with OPMD. In this perspective paper, several experts discuss the main challenges in oral cancer prevention, in particular the need to (i) to define an OPMD classification system by integrating new pathological and molecular characteristics, aiming (ii) to better identify OPMD at high risk of malignant transformation, and (iii) to develop treatment strategies to eradicate OPMD or prevent malignant transformation.
- Published
- 2022
- Full Text
- View/download PDF
34. Delto-acromial artery perforator flap combined with a radial forearm free flap as a "vascular bridge" for nasal reconstruction.
- Author
-
Bouaoud J, Benmoussa N, Honart JF, Temam S, Moya-Plana A, Leymarie N, and Kolb F
- Subjects
- Arteries surgery, Forearm blood supply, Forearm surgery, Humans, Free Tissue Flaps transplantation, Perforator Flap blood supply, Perforator Flap surgery, Plastic Surgery Procedures methods
- Abstract
Nasal reconstruction for total rhinectomy is challenging, especially if locoregional flaps are unavailable. Herein, we report the case of a nasal reconstruction combining a forearm free flap as "vascular bridge" and a Delto-Acromial Artery Perforator (DAAP) flap in its free form. The forearm free flap was used to restore missing elements of the nasal lining while the distal part of the radial pedicle has served as a donor vessel for the DAAP free flap which restores the nasal covering. A chondrocostal graft was used as a nasal framework. The nasal aspect at 24 months postop support the patient's satisfaction. The main advantages of the DAAP Flap are the pliability, relative hairless nature, skin thinness and its geographical proximity with the nose avoiding major dyschromia. Moreover, the anatomy consistency makes it easier to harvest, the underlying muscles are respected, and it allows for tension free primary closure without shoulder movement limitation., (Copyright © 2021 Elsevier Masson SAS. All rights reserved.)
- Published
- 2022
- Full Text
- View/download PDF
35. MEK1/2 inhibition transiently alters the tumor immune microenvironment to enhance immunotherapy efficacy against head and neck cancer.
- Author
-
Prasad M, Zorea J, Jagadeeshan S, Shnerb AB, Mathukkada S, Bouaoud J, Michon L, Novoplansky O, Badarni M, Cohen L, Yegodayev KM, Tzadok S, Rotblat B, Brezina L, Mock A, Karabajakian A, Fayette J, Cohen I, Cooks T, Allon I, Dimitstein O, Joshua B, Kong D, Voronov E, Scaltriti M, Carmi Y, Conde-Lopez C, Hess J, Kurth I, Morris LGT, Saintigny P, and Elkabets M
- Subjects
- Animals, CD8-Positive T-Lymphocytes, Cell Line, Tumor, Epithelial-Mesenchymal Transition, Humans, Immunotherapy, Mice, Head and Neck Neoplasms drug therapy, Tumor Microenvironment
- Abstract
Background: Although the mitogen-activated protein kinases (MAPK) pathway is hyperactive in head and neck cancer (HNC), inhibition of MEK1/2 in HNC patients has not shown clinically meaningful activity. Therefore, we aimed to characterize the effect of MEK1/2 inhibition on the tumor microenvironment (TME) of MAPK-driven HNC, elucidate tumor-host interaction mechanisms facilitating immune escape on treatment, and apply rationale-based therapy combination immunotherapy and MEK1/2 inhibitor to induce tumor clearance., Methods: Mouse syngeneic tumors and xenografts experiments were used to analyze tumor growth in vivo. Single-cell cytometry by time of flight, flow cytometry, and tissue stainings were used to profile the TME in response to trametinib (MEK1/2 inhibitor). Co-culture of myeloid-derived suppressor cells (MDSC) with CD8
+ T cells was used to measure immune suppression. Overexpression of colony-stimulating factor-1 (CSF-1) in tumor cells was used to show the effect of tumor-derived CSF-1 on sensitivity to trametinib and anti-programmed death- 1 (αPD-1) in mice. In HNC patients, the ratio between CSF-1 and CD8A was measured to test the association with clinical benefit to αPD-1 and αPD-L1 treatment., Results: Using preclinical HNC models, we demonstrated that treatment with trametinib delays HNC initiation and progression by reducing tumor cell proliferation and enhancing the antitumor immunity of CD8+ T cells. Activation of CD8+ T cells by supplementation with αPD-1 antibody eliminated tumors and induced an immune memory in the cured mice. Mechanistically, an early response to trametinib treatment sensitized tumors to αPD-1-supplementation by attenuating the expression of tumor-derived CSF-1, which reduced the abundance of two CSF-1R+ CD11c+ MDSC populations in the TME. In contrast, prolonged treatment with trametinib abolished the antitumor activity of αPD-1, because tumor cells undergoing the epithelial to mesenchymal transition in response to trametinib restored CSF-1 expression and recreated an immune-suppressive TME., Conclusion: Our findings provide the rationale for testing the trametinib/αPD-1 combination in HNC and highlight the importance of sensitizing tumors to αPD-1 by using MEK1/2 to interfere with the tumor-host interaction. Moreover, we describe the concept that treatment of cancer with a targeted therapy transiently induces an immune-active microenvironment, and supplementation of immunotherapy during this time further activates the antitumor machinery to cause tumor elimination., Competing Interests: Competing interests: JH was paid consultant for Bristol-Myers Squibb and MSD Sharpe & Dohme and has received other commercial research support from CureVac A G and PROGEN Biotechnik, Research funding from AstraZeneca, outside the scope of this work (to LGTM). LGTM is an inventor on a patent held by Memorial Sloan Kettering related to tumor mutational burden and immunotherapy. MS is an employee and stockholder of Astra Zeneca. JF received Honoraria from Astra Zeneca, Bristol-Myers Squibb, Merk Sharp & Dohme, Merck Serono, Innate pharma, Roche, serve as an advisor in, Astra Zeneca, Bristol-Myers Squibb, Merk Sharp & Dohme, Merck Serono, Innate pharma, Roche, has a research fund by Bristol-Myers Squibb, and had travel grants from Astra Zeneca, Bristol-Myers Squibb, Merk Sharp & Dohme., (© Author(s) (or their employer(s)) 2022. Re-use permitted under CC BY. Published by BMJ.)- Published
- 2022
- Full Text
- View/download PDF
36. Comment to: "Head and neck osteosarcomas: An analysis of treatment trends and survival outcomes in the United States (2004-2016)".
- Author
-
Bouaoud J, Schouman T, and Bertolus C
- Subjects
- Head, Humans, Neck, Retrospective Studies, Survival Analysis, United States epidemiology, Head and Neck Neoplasms epidemiology, Head and Neck Neoplasms therapy, Osteosarcoma epidemiology, Osteosarcoma therapy
- Published
- 2021
- Full Text
- View/download PDF
37. Humanitarian Maxillofacial Mission's Success Requires Experienced Surgeons, Careful Planning, and Meeting With the Local's Care Needs.
- Author
-
Bouaoud J, Ndiaye MM, Benassarou M, Toure S, Schouman T, and Bertolus C
- Subjects
- Humans, Retrospective Studies, Medical Missions, Plastic Surgery Procedures, Surgeons, Surgery, Oral
- Abstract
Purpose: Meeting with local needs of low- and middle-income countries during maxillofacial humanitarian mission is not easy. This article aimed to report on 5 years of experience in humanitarian maxillofacial surgery missions. In addition, several key points for best practices and meeting the medical needs of local populations are discussed., Methods: In this retrospective case series, all medical charts of patients managed during humanitarian maxillofacial surgery missions organized within the department of maxillofacial surgery of Le Dantec Hospital (Senegal) were analyzed. Disease characteristics, treatments modality, and outcomes were reviewed. Moreover, missions planning and costs were studied., Results: Between 2015 and 2018, 5 humanitarian missions were organized totalizing 177 patients, one-third of which were treated surgically. Tumors (35%) and sequelae from previous surgeries, cancrum oris or trauma (24%) were the most frequently treated disorders. Most patients were treated with free flap reconstructions (35%). Postoperative complications were observed for only 3 patients (5%). With a median follow-up of 13 months, no sequelae requiring specific treatment were observed. The estimated total cost for each mission was $39,000., Conclusion: In order to benefit both the locals and the volunteers, humanitarian maxillofacial missions should be carefully planned and volunteers appropriately prepared. Other keys to the success of such missions are setting up training and support programs, reflecting upon ethical considerations, understanding local cultural customs and ensuring mutual respect with the locals. Frequent self-evaluation and long-term mission sustainability are critical. Finally, mission costs should be evaluated., (Copyright © 2021 The American Association of Oral and Maxillofacial Surgeons. Published by Elsevier Inc. All rights reserved.)
- Published
- 2021
- Full Text
- View/download PDF
38. In-house 3D printing: Why, when, and how? Overview of the national French good practice guidelines for in-house 3D-printing in maxillo-facial surgery, stomatology, and oral surgery.
- Author
-
Khonsari RH, Adam J, Benassarou M, Bertin H, Billotet B, Bouaoud J, Bouletreau P, Garmi R, Gellée T, Haen P, Ketoff S, Lescaille G, Louvrier A, Lutz JC, Makaremi M, Nicot R, Pham-Dang N, Praud M, Saint-Pierre F, Schouman T, Sicard L, Simon F, Wojcik T, and Meyer C
- Subjects
- France, Humans, Printing, Three-Dimensional, Oral Medicine, Oral Surgical Procedures, Surgery, Oral
- Abstract
3D-printing is part of the daily practice of maxillo-facial surgeons, stomatologists and oral surgeons. To date, no French health center is producing in-house medical devices according to the new European standards. Based on all the evidence-based data available, a group of experts from the French Society of Stomatology, Maxillo-Facial Surgery and Oral Surgery (Société Française de Chirurgie Maxillofaciale, Stomatologie et Chirurgie Orale, SFSCMFCO), provide good practice guidelines for in-house 3D-printing in maxillo-facial surgery, stomatology, and oral surgery. Briefly, technical considerations related to printers and CAD software, which were the main challenges in the last ten years, are now nearly trivial questions. The central current issues when planning the implementation of an in-house 3D-printing platform are economic and regulatory. Successful in-house 3D platforms rely on close collaborations between health professionals and engineers, backed by regulatory and logistic specialists. Several large-scale academic projects across France will soon provide definitive answers to governance and economical questions related to the use of in-house 3D printing., (Copyright © 2021 Elsevier Masson SAS. All rights reserved.)
- Published
- 2021
- Full Text
- View/download PDF
39. DIVA, a 3D virtual reality platform, improves undergraduate craniofacial trauma education.
- Author
-
Bouaoud J, El Beheiry M, Jablon E, Schouman T, Bertolus C, Picard A, Masson JB, and Khonsari RH
- Subjects
- Humans, Imaging, Three-Dimensional, Software, Tomography, X-Ray Computed, Virtual Reality
- Abstract
Craniofacial fractures management is challenging to teach due to the complex anatomy of the head, even when using three-dimensional CT-scan images. DIVA is a software allowing the straightforward visualization of CT-scans in a user-friendly three-dimensional virtual reality environment. Here, we assess DIVA as an educational tool for craniofacial trauma for undergraduate medical students. Three craniofacial trauma cases (jaw fracture, naso-orbital-ethmoid complex fracture and Le Fort 3 fracture) were submitted to 50 undergraduate medical students, who had to provide diagnoses and treatment plans. Each student then filled an 8-item questionnaire assessing satisfaction, potential benefit, ease of use and tolerance. Additionally, 4 postgraduate students were requested to explore these cases and to place 6 anatomical landmarks on both virtual reality renderings and usual slice-based three-dimensional CT-scan visualizations. High degrees of satisfaction (98%) without specific tolerance issues (86%) were reported. The potential benefit in a better understanding of craniofacial trauma using virtual reality was reported by almost all students (98%). Virtual reality allowed a reliable localization of key anatomical landmarks when compared with standard three-dimensional CT-scan visualization. Virtual reality interfaces such DIVA are beneficial to medical students for a better understanding of craniofacial trauma and allow a reliable rendering of craniofacial anatomy., (Copyright © 2020 Elsevier Masson SAS. All rights reserved.)
- Published
- 2021
- Full Text
- View/download PDF
40. Longitudinal assessment of PD-L1 expression and gene expression profiles in patients with head and neck cancer reveals temporal heterogeneity.
- Author
-
Karabajakian A, Bouaoud J, Michon L, Kamal M, Crozes C, Zrounba P, Auclair-Perossier J, Gadot N, Attignon V, Le Tourneau C, Benzerdjeb N, Fayette J, and Saintigny P
- Subjects
- Humans, Neoplasm Recurrence, Local genetics, Transcriptome, B7-H1 Antigen genetics, Head and Neck Neoplasms genetics, Squamous Cell Carcinoma of Head and Neck genetics
- Abstract
Background: Programmed death-ligand 1 (PD-L1) is the most validated predictive biomarker used for the treatment of head and neck squamous cell carcinoma (HNSCC) with immune checkpoint inhibitors (ICI). Several gene expression-based signatures surrogate of the activation of IFN-gamma pathway and of the presence of tertiary lymphoid structures (TLS) have also been proposed as potential biomarkers. While they may have a potential therapeutic implication, the longitudinal changes of either PD-L1 or gene expression profiles between the initial and recurrent HNSCC lesions is unknown., Methods: PD-L1 immunohistochemistry (IHC) and targeted RNA-sequencing of 2,549 transcripts were analyzed on paired specimens from the initial diagnosis and recurrent HNSCC. PD-L1 status was defined using the combined positive score (CPS). PD-L1 mRNA levels were compared with protein expression levels by IHC. Enrichment scores of surrogate signatures for TLS and IFN-gamma (IFN-γ) pathway activation were computed using the single sample gene set enrichment analysis (ssGSEA)., Results: PD-L1 status was 64% (21/33) concordant between the initial and recurrent lesions using a CPS 1 threshold and 67% (22/33) concordant using a CPS 20 threshold. CPS score was associated with PD-L1 gene expression levels. There was a 43% (15/35) and 66% (23/35) concordance for the IFN-γ and TLS signature scores, respectively., Conclusion: Our study reveals temporal heterogeneity of PD-L1 status and TLS/IFN-γ gene expression surrogates in HNSCC that need to be considered when interpreting biomarker studies., (Copyright © 2021 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
- Published
- 2021
- Full Text
- View/download PDF
41. Early changes in the immune microenvironment of oral potentially malignant disorders reveal an unexpected association of M2 macrophages with oral cancer free survival.
- Author
-
Bouaoud J, Foy JP, Tortereau A, Michon L, Lavergne V, Gadot N, Boyault S, Valantin J, De Souza G, Zrounba P, Bertolus C, Bendriss-Vermare N, and Saintigny P
- Subjects
- Animals, Humans, Macrophages, Mice, Tumor Microenvironment, Carcinoma, Squamous Cell genetics, Head and Neck Neoplasms, Mouth Neoplasms genetics
- Abstract
Understanding the dynamics of the immune microenvironment is critical to the development of immuno-based strategies for the prevention of oral potentially malignant disorders transformation to oral squamous cell carcinoma (OSCC). We used laser capture microdissection and RNA-sequencing to profile the expression of 13 matched pairs of epithelial versus stromal compartments from normal mucosa, hyperplasia, dysplasia, and invasive tumors in the 4-nitroquinolein (4-NQO) murine model of oral carcinogenesis. Genes differentially expressed at each step of transformation were defined. Immune cell deconvolution and enrichment scores of various biological processes including immune-related ones were computed. Immunohistochemistry was also performed to characterize the immune infiltrates by T-cells (T-cells CD3+, helper CD4+, cytotoxic CD8+, regulatory FoxP3+), B-cells (B220+), and macrophages (M1 iNOS+, M2 CD163+) at each histological step. Enrichment of three independent M2 macrophages signatures were computed in 86 oral leukoplakia with available clinical outcome. Most gene expression changes were observed in the stromal compartment and related to immune biological processes. Immune cell deconvolution identified infiltration by the macrophage population as the most important quantitatively especially at the stage of dysplasia. In 86 patients with oral leukoplakia, three M2 macrophages signatures were independently associated with improved oral cancer-free survival. This study provides a better understanding of the dynamics of the immune microenvironment during oral carcinogenesis and highlights an unexpected association of M2 macrophages gene expression signatures with oral cancer free survival in patients with oral leukoplakia., (© 2021 The Author(s). Published with license by Taylor & Francis Group, LLC.)
- Published
- 2021
- Full Text
- View/download PDF
42. Bilateral bifid condyles: A rare etiology of temporomandibular joint disorders.
- Author
-
Bettoni J, Olivetto M, Bouaoud J, Duisit J, and Dakpé S
- Subjects
- Adult, Humans, Mandibular Condyle diagnostic imaging, Radiography, Panoramic, Temporomandibular Joint, Tomography, X-Ray Computed, Young Adult, Temporomandibular Joint Disorders diagnostic imaging, Temporomandibular Joint Disorders etiology, Tooth Ankylosis
- Abstract
Background : Bifid mandibular condyle (BMC) is a rare etiology of temporomandibular joint (TMJ) disorders characterized by a duplication of the head of the mandibular condyle. Case report : The authors report the case of a 20-year-old patient complaining of a painful and clicking TMJ and mandibular hypomobility, which had been progressing for several months. Radiological investigations (dental panoramic radiograph and X-ray CT scan) revealed right and left abnormalities of the TMJ due to bilateral BMC requiring surgical management. Conclusion : Despite a prevalence of 0.31% to 1.82% and the controversies surrounding its pathophysiology, maxillofacial surgeons should be aware of BMC to avoid misdiagnosis related to the clinical presentation (pain, clicking, hypomobility, or ankylosis) and provide adequate management.
- Published
- 2021
- Full Text
- View/download PDF
43. Precision Medicine Approaches to Overcome Resistance to Therapy in Head and Neck Cancers.
- Author
-
Ortiz-Cuaran S, Bouaoud J, Karabajakian A, Fayette J, and Saintigny P
- Abstract
Head and neck squamous cell carcinoma (HNSCC) is the sixth most incident cancer worldwide. More than half of HNSCC patients experience locoregional or distant relapse to treatment despite aggressive multimodal therapeutic approaches that include surgical resection, radiation therapy, and adjuvant chemotherapy. Before the arrival of immunotherapy, systemic chemotherapy was previously employed as the standard first-line protocol with an association of cisplatin or carboplatin plus 5-fluorouracil plus cetuximab (anti-EFGR antibody). Unfortunately, acquisition of therapy resistance is common in patients with HNSCC and often results in local and distant failure. Despite our better understanding of HNSCC biology, no other molecular-targeted agent has been approved for HNSCC. In this review, we outline the mechanisms of resistance to the therapeutic strategies currently used in HNSCC, discuss combination treatment strategies to overcome them, and summarize the therapeutic regimens that are presently being evaluated in early- and late-phase clinical trials., Competing Interests: PS receives research grants from HTG Molecular Diagnostics, Inivata, Bristol-Myers Squibb, Roche Molecular Diagnostics, Roche, AstraZeneca, Novartis, Bristol-Myers Squibb Foundation, and Illumina. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Ortiz-Cuaran, Bouaoud, Karabajakian, Fayette and Saintigny.)
- Published
- 2021
- Full Text
- View/download PDF
44. The 4-NQO mouse model: An update on a well-established in vivo model of oral carcinogenesis.
- Author
-
Bouaoud J, De Souza G, Darido C, Tortereau A, Elkabets M, Bertolus C, and Saintigny P
- Subjects
- 4-Nitroquinoline-1-oxide toxicity, Animals, Carcinogenesis, Carcinogens toxicity, Mice, Tumor Microenvironment, Carcinoma, Squamous Cell chemically induced, Carcinoma, Squamous Cell genetics, Mouth Neoplasms chemically induced
- Abstract
The early detection and management of oral premalignant lesions (OPMDs) improve their outcomes. Animal models that mimic histological and biological processes of human oral carcinogenesis may help to improve the identification of OPMD at-risk of progression into oral squamous cell carcinoma and to develop preventive strategies for the entire field of cancerization. No animal model is perfectly applicable for investigating human oral carcinogenesis. However, the 4-nitroquinoline 1-oxide (4-NQO) mouse model is well established and mimics several morphological, histological, genomic and molecular features of human oral carcinogenesis. Some of the reasons for the success of this model include its reproducible experimental conditions with limited variation, the possibility of realizing longitudinal studies with invasive intervention or gene manipulation, and sample availability for all stages of oral carcinogenesis, especially premalignant lesions. Moreover, the role of histological and molecular alterations in the field of cancerization (i.e., macroscopically healthy mucosa exposed to a carcinogen) during oral carcinogenesis can be easily explored using this model. In this review, we discuss the advantages and drawbacks of this model for studying human oral carcinogenesis. In summary, the 4-NQO-induced murine oral cancer model is relevant for investigating human oral carcinogenesis, including the immune microenvironment, and for evaluating therapeutic and chemoprevention agents., (Copyright © 2021 Elsevier Inc. All rights reserved.)
- Published
- 2021
- Full Text
- View/download PDF
45. Use of an occlusal splint and intraoperative imaging with an intraoral approach in the management of mandibular subcondylar fractures.
- Author
-
Olivetto M, Bettoni J, Bouaoud J, Testelin S, Dakpé S, Lefranc M, and Devauchelle B
- Subjects
- Fracture Fixation, Internal, Humans, Mandibular Condyle, Occlusal Splints, Open Fracture Reduction, Mandibular Fractures
- Abstract
Introduction: To evaluate the feasibility of safely managing subcondylar fractures using an original surgical procedure combining an intraoral approach, the use of a custom-made occlusal overlay splint, and intraoperative imaging., Materials and Methods: Condylar fragment was freed from surrounding soft tissues, was laterally exposed to the ramus, and a miniplate was fixed in place for osteosynthesis. An overlay splint maintaining the dental occlusion was used to facilitate reduction and stabilization during fixation. Intraoperative monitoring by cone-beam computed tomography (CBCT) was performed before completing the fixation., Results: Between November 2018 and June 2019, 10 patients were treated using this procedure. The median length of the proximal condylar fragment was 29 mm (range 24-39 min). Five patients had an associated mandibular fracture. The median duration of the condylar fracture surgery was 54.5 min (range 38-79 min). All patients had satisfactory reduction and osteosynthesis with no complications., Conclusion: It is feasible to safely manage subcondylar fractures with this surgical procedure that could facilitate open reduction using intraoral approaches. Occlusal splints maintain downward pressure on the rami bilaterally and symmetrically, helping to anatomically reposition condylar process fractures. Intraoperative imaging is used to monitor this step., (Copyright © 2020 European Association for Cranio-Maxillo-Facial Surgery. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2020
- Full Text
- View/download PDF
46. Genomics and precision surgery for head and neck squamous cell carcinoma.
- Author
-
Galmiche A, Saidak Z, Bouaoud J, Mirghani H, Page C, Dakpé S, and Clatot F
- Subjects
- Genomics methods, Humans, Molecular Targeted Therapy methods, Precision Medicine methods, Carcinoma, Squamous Cell genetics, Carcinoma, Squamous Cell surgery, Head and Neck Neoplasms genetics, Head and Neck Neoplasms surgery, Squamous Cell Carcinoma of Head and Neck genetics, Squamous Cell Carcinoma of Head and Neck surgery
- Abstract
The identification of the biological determinants that shape the response of tumors to medical therapies offers perspectives for better patient stratification and therapeutic targeting. Here, we discuss how genomics could help to improve the surgical treatment of head and neck squamous cell carcinoma (HNSCC). We examine the potential use of genomic analyses for: i) refining and standardizing the indications for surgery, ii) the choice of surgical procedure, and iii) the follow-up of patients with resected tumors. We highlight the studies that used genomics to explore the contribution of tumor biology to the outcome of surgery. We discuss the important developments that are challenging current surgical practice in HNSCC, such as neoadjuvant immunotherapy and the analysis of circulating DNA. Genomic analyses provide practical tools that could help improve the pathological diagnosis and staging of HNSCC, and increase the appreciation of the importance of tumor biology in the outcome of surgery. Identification of biomarkers will likely contribute to a move toward precision surgery of HNSCC, i.e. the personalization of surgical practice based on tumor biology., Competing Interests: Declaration of competing interest The authors have no conflicts of interest to report., (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Published
- 2020
- Full Text
- View/download PDF
47. CDYL2 Epigenetically Regulates MIR124 to Control NF-κB/STAT3-Dependent Breast Cancer Cell Plasticity.
- Author
-
Siouda M, Dujardin AD, Barbollat-Boutrand L, Mendoza-Parra MA, Gibert B, Ouzounova M, Bouaoud J, Tonon L, Robert M, Foy JP, Lavergne V, Manie SN, Viari A, Puisieux A, Ichim G, Gronemeyer H, Saintigny P, and Mulligan P
- Abstract
Epigenetic deregulation of gene transcription is central to cancer cell plasticity and malignant progression but remains poorly understood. We found that the uncharacterized epigenetic factor chromodomain on Y-like 2 (CDYL2) is commonly over-expressed in breast cancer, and that high CDYL2 levels correlate with poor prognosis. Supporting a functional role for CDYL2 in malignancy, it positively regulated breast cancer cell migration, invasion, stem-like phenotypes, and epithelial-to-mesenchymal transition. CDYL2 regulation of these plasticity-associated processes depended on signaling via p65/NF-κB and STAT3. This, in turn, was downstream of CDYL2 regulation of MIR124 gene transcription. CDYL2 co-immunoprecipitated with G9a/EHMT2 and GLP/EHMT1 and regulated the chromatin enrichment of G9a and EZH2 at MIR124 genes. We propose that CDYL2 contributes to poor prognosis in breast cancer by recruiting G9a and EZH2 to epigenetically repress MIR124 genes, thereby promoting NF-κB and STAT3 signaling, as well as downstream cancer cell plasticity and malignant progression., Competing Interests: Declaration of Interests The authors have no conflicts of interest to declare., (Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2020
- Full Text
- View/download PDF
48. Excessive ossification of the bandeau in Crouzon and Apert syndromes.
- Author
-
Bouaoud J, Hennocq Q, Paternoster G, James S, Arnaud E, and Khonsari RH
- Subjects
- Child, Frontal Bone, Humans, Osteogenesis, Syndrome, Acrocephalosyndactylia, Craniofacial Dysostosis
- Abstract
Background: The study aimed at assessing the variations in thickness of the supra-orbital bar in Crouzon (CS) and Apert syndromes (AS) before and after fronto-facial monobloc advancement (FFMBA) using CT-scan data., Methods: All CS or AS patients who underwent FFMBA between 2008 and 2018 with available clinical and CT-scan data were included. Frontal bone thickness was assessed at five locations of the supra-orbital bar using the Half-Maximum-Height protocol and plotted over the bone surface., Results: We included 210 CT-scans from 25 CS to 10 AS patients and 25 controls. Before FFMBA, CS children only had a significantly thicker frontal bone (+0.772 mm ± 0.312, p = 0.017). After FFMBA, a significant increase in frontal bone thickness was reported for CS at 3 months (+0.637 mm ± 0.141, p < 0.001), 6 months (+0.910 mm ± 0.120, p < 0.001) and 12 months (+1.099 mm ± 0.124, p < 0.001) post-operatively as well as in AS at 1 month (+2.069 mm ± 0.441, p < 0.001), 6 months (+1.247 mm ± 0.406, p = 0.003) and 12 months (+2.360 mm ± 0.284, p < 0.001) post-operatively. For both syndromes, age at FFMBA and specific FGFR2 mutations significantly influenced post-operative frontal bone thickness., Conclusions: Craniofacial surgeons should be aware of the potential need for secondary surgery of the supra-orbital after FFMBA in CS and AS. Furthermore, a thicker supra-orbital bar is part of the CS phenotype, illustrating the role of FGFR2 in craniofacial growth., (Copyright © 2020 European Association for Cranio-Maxillo-Facial Surgery. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2020
- Full Text
- View/download PDF
49. Endosteal blood supply of the mandible: anatomical study of nutrient vessels in the condylar neck accessory foramina.
- Author
-
Olivetto M, Bettoni J, Duisit J, Chenin L, Bouaoud J, Dakpé S, Devauchelle B, and Lengelé B
- Subjects
- Cadaver, Dissection, Female, Fixatives, Humans, Latex, Male, Mandible anatomy & histology, Mandible blood supply, Mandible surgery, Mandibular Condyle anatomy & histology, Mandibular Condyle surgery, Maxillary Artery surgery, Tissue Fixation methods, Mandibular Condyle blood supply, Maxillary Artery anatomy & histology
- Abstract
Purpose: In the mandible, the condylar neck vascularization is commonly described as mainly periosteal; while the endosteal contribution is still debated, with very limited anatomical studies. Previous works have shown the contribution of nutrient vessels through accessory foramina and their contribution in the blood supply of other parts of the mandible. Our aim was to study the condylar neck's blood supply from nutrient foramina., Methods: Six latex-injected heads were dissected and two hundred mandibular condyles were observed on dry mandibles searching for accessory bone foramina., Results: Latex-injected dissections showed a direct condylar medular arterial supply through foramina. On dry mandibles, these foramina were most frequently observed in the pterygoid fovea in 91% of cases. However, two other accessory foramina areas were identified on the lateral and medial sides of the mandibular condylar process, confirming the vascular contribution of transverse facial and maxillary arteries., Conclusions: The maxillary artery indeed provided both endosteal and periosteal blood supply to the condylar neck, with three different branches: an intramedullary ascending artery (arising from the inferior alveolar artery), a direct nutrient branch and some pterygoid osteomuscular branches.
- Published
- 2020
- Full Text
- View/download PDF
50. Lack of efficacy of neoadjuvant chemotherapy in adult patients with maxillo-facial high-grade osteosarcomas: A French experience in two reference centers.
- Author
-
Bouaoud J, Beinse G, Epaillard N, Amor-Sehlil M, Bidault F, Brocheriou I, Hervé G, Spano JP, Janot F, Boudou-Rouquette P, Benassarou M, Schouman T, Goudot P, Malouf G, Goldwasser F, and Bertolus C
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Cancer Care Facilities statistics & numerical data, Chemotherapy, Adjuvant adverse effects, Chemotherapy, Adjuvant methods, Disease-Free Survival, Female, Follow-Up Studies, France epidemiology, Humans, Kaplan-Meier Estimate, Male, Margins of Excision, Maxilla diagnostic imaging, Maxilla drug effects, Maxilla pathology, Maxilla surgery, Maxillary Neoplasms diagnosis, Maxillary Neoplasms mortality, Maxillary Neoplasms pathology, Middle Aged, Neoadjuvant Therapy methods, Neoplasm Recurrence, Local pathology, Osteosarcoma diagnosis, Osteosarcoma mortality, Osteosarcoma pathology, Retrospective Studies, Tertiary Care Centers statistics & numerical data, Tumor Burden, Young Adult, Maxillary Neoplasms therapy, Neoadjuvant Therapy adverse effects, Neoplasm Recurrence, Local epidemiology, Osteosarcoma therapy
- Abstract
Introduction: Neoadjuvant chemotherapy (neo-CT) for osteosarcomas is the standard of care. Management of maxillo-facial osteosarcomas (MFOS) is challenging. In this rare disease, we collected a large cohort of patients with the aim to report the histological and radiological local response rates to neo-CT., Patients and Methods: All consecutive adult patients treated between 2001 and 2016 in two French sarcoma referral centers (Pitié-Salpêtrière Hospital, APHP, RESAP France and Gustave Roussy Institute France), for a histologically proved MFOS were included. Clinical, histological and radiological data were independently reviewed. Tumor response to neo-CT was assessed clinically, radiologically with independent review using RECIST v1.1 criterion and pathologically (percentage of necrosis). Multivariate analysis was done for outcomes, tumor response and disease-free survival (DFS)., Results: A total of 35 high grade MFOS were collected. The clinical tumor response was 4% (1/24 receiving neo-CT), the radiological response was 0% (0/18 with available data) and the pathological response was 5% (1/20 with available data). Three patients (12.5%) initially resectable became unresectable due to clinical and radiological progression during neo-CT. Tumor size and R0 (clear margins) surgical resections were significantly associated with DFS., Conclusion: MFOS is a rare disease. This large retrospective cohort of MFOS indicates the lack of benefit and potentially deleterious effects of neo-CT. We suggest privileging primary surgery in initially localized resectable MFOS. The benefit of adjuvant chemotherapy should be prospectively studied., (Copyright © 2019 Elsevier Ltd. All rights reserved.)
- Published
- 2019
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.