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465 results on '"Bottino, Marco C."'

19. Biodegradable electrospun poly(L‐lactide‐co‐ε‐caprolactone)/polyethylene glycol/bioactive glass composite scaffold for bone tissue engineering

Author

de Souza, Joyce R., primary, Cardoso, Lais M., additional, de Toledo, Priscila T. A., additional, Rahimnejad, Maedeh, additional, Kito, Letícia T., additional, Thim, Gilmar P., additional, Campos, Tiago M. B., additional, Borges, Alexandre L. S., additional, and Bottino, Marco C., additional

Published
2024
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21. Functionalization of PCL-Based Fiber Scaffolds with Different Sources of Calcium and Phosphate and Odontogenic Potential on Human Dental Pulp Cells

Author

Anselmi, Caroline, primary, Mendes Soares, Igor Paulino, additional, Mota, Rafaella Lara Maia, additional, Leite, Maria Luísa, additional, Ribeiro, Rafael Antonio de Oliveira, additional, Fernandes, Lídia de Oliveira, additional, Bottino, Marco C., additional, de Souza Costa, Carlos Alberto, additional, and Hebling, Josimeri, additional

Published
2024
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23. Development of Cerium Oxide-Laden GelMA/PCL Scaffolds for Periodontal Tissue Engineering.

Author

Aminmansour, Sahar, Cardoso, Lais M., Anselmi, Caroline, de Carvalho, Ana Beatriz Gomes, Rahimnejad, Maedeh, and Bottino, Marco C.

Subjects
FOURIER transform infrared spectroscopy, MESENCHYMAL stem cells, YOUNG'S modulus, TISSUE engineering, ANALYTICAL chemistry
Abstract

This study investigated gelatin methacryloyl (GelMA) and polycaprolactone (PCL) blend scaffolds incorporating cerium oxide (CeO) nanoparticles at concentrations of 0%, 5%, and 10% w/w via electrospinning for periodontal tissue engineering. The impact of photocrosslinking on these scaffolds was evaluated by comparing crosslinked (C) and non-crosslinked (NC) versions. Methods included Fourier transform infrared spectroscopy (FTIR) for chemical analysis, scanning electron microscopy (SEM) for fiber morphology/diameters, and assessments of swelling capacity, degradation profile, and biomechanical properties. Biological evaluations with alveolar bone-derived mesenchymal stem cells (aBMSCs) and human gingival fibroblasts (HGFs) encompassed tests for cell viability, mineralized nodule deposition (MND), and collagen production (CP). Statistical analysis was performed using Kruskal–Wallis or ANOVA/post-hoc tests (α = 5%). Results indicate that C scaffolds had larger fiber diameters (~250 nm) compared with NC scaffolds (~150 nm). NC scaffolds exhibited higher swelling capacities than C scaffolds, while both types demonstrated significant mass loss (~50%) after 60 days (p < 0.05). C scaffolds containing CeO showed increased Young's modulus and tensile strength than NC scaffolds. Cells cultured on C scaffolds with 10% CeO exhibited significantly higher metabolic activity (>400%, p < 0.05) after 7 days among all groups. Furthermore, CeO-containing scaffolds promoted enhanced MND by aBMSCs (>120%, p < 0.05) and increased CP in 5% CeO scaffolds for both variants (>180%, p < 0.05). These findings underscore the promising biomechanical properties, biodegradability, cytocompatibility, and enhanced tissue regenerative potential of CeO-loaded GelMA/PCL scaffolds for periodontal applications. [ABSTRACT FROM AUTHOR]

Published
2024
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37. Nanoscale β-TCP-Laden GelMA/PCL Composite Membrane for Guided Bone Regeneration

Author

Mahmoud, Abdel H, Han, Yuanyuan, Dal-Fabbro, Renan, Daghrery, Arwa, Xu, Jinping, Kaigler, Darnell, Bhaduri, Sarit B, Malda, Jos, Bottino, Marco C, CS_Locomotion, and Equine Musculoskeletal Biology

Subjects
Electrospinning, Gelatin, Regeneration, Tissue engineering, Extracellular matrix, Bone
Abstract

Major advances in the field of periodontal tissue engineering have favored the fabrication of biodegradable membranes with tunable physical and biological properties for guided bone regeneration (GBR). Herein, we engineered innovative nanoscale beta-tricalcium phosphate (β-TCP)-laden gelatin methacryloyl/polycaprolactone (GelMA/PCL-TCP) photocrosslinkable composite fibrous membranes via electrospinning. Chemo-morphological findings showed that the composite microfibers had a uniform porous network and β-TCP particles successfully integrated within the fibers. Compared with pure PCL and GelMA/PCL, GelMA/PCL-TCP membranes led to increased cell attachment, proliferation, mineralization, and osteogenic gene expression in alveolar bone-derived mesenchymal stem cells (aBMSCs). Moreover, our GelMA/PCL-TCP membrane was able to promote robust bone regeneration in rat calvarial critical-size defects, showing remarkable osteogenesis compared to PCL and GelMA/PCL groups. Altogether, the GelMA/PCL-TCP composite fibrous membrane promoted osteogenic differentiation of aBMSCs in vitro and pronounced bone formation in vivo. Our data confirmed that the electrospun GelMA/PCL-TCP composite has a strong potential as a promising membrane for guided bone regeneration.

Published
2023

41. Tissue-specific melt electrowritten polymeric scaffolds for coordinated regeneration of soft and hard periodontal tissues

Author

Equine Musculoskeletal Biology, dES RMSC, Daghrery, Arwa, Ferreira, Jessica A, Xu, Jinping, Golafshan, Nasim, Kaigler, Darnell, Bhaduri, Sarit B, Malda, Jos, Castilho, Miguel, Bottino, Marco C, Equine Musculoskeletal Biology, dES RMSC, Daghrery, Arwa, Ferreira, Jessica A, Xu, Jinping, Golafshan, Nasim, Kaigler, Darnell, Bhaduri, Sarit B, Malda, Jos, Castilho, Miguel, and Bottino, Marco C

Published
2023

42. Composite Graded Melt Electrowritten Scaffolds for Regeneration of the Periodontal Ligament-to-Bone Interface

Author

CS_Locomotion, Equine Musculoskeletal Biology, Locomotion - Equine Sciences, Golafshan, Nasim, Castilho, Miguel, Daghrery, Arwa, Alehosseini, Morteza, van de Kemp, Tom, Krikonis, Konstantinos, de Ruijter, Mylene, Dal-Fabbro, Renan, Dolatshahi-Pirouz, Alireza, Bhaduri, Sarit B, Bottino, Marco C, Malda, Jos, CS_Locomotion, Equine Musculoskeletal Biology, Locomotion - Equine Sciences, Golafshan, Nasim, Castilho, Miguel, Daghrery, Arwa, Alehosseini, Morteza, van de Kemp, Tom, Krikonis, Konstantinos, de Ruijter, Mylene, Dal-Fabbro, Renan, Dolatshahi-Pirouz, Alireza, Bhaduri, Sarit B, Bottino, Marco C, and Malda, Jos

Published
2023

43. Unveiling the Potential of Melt Electrowriting in Regenerative Dental Medicine

Author

Equine Musculoskeletal Biology, dES RMSC, Locomotion - Equine Sciences, Daghrery, Arwa, Araújo, Isaac J. de Souza, Castilho, Miguel, Malda, Jos, Bottino, Marco C., Equine Musculoskeletal Biology, dES RMSC, Locomotion - Equine Sciences, Daghrery, Arwa, Araújo, Isaac J. de Souza, Castilho, Miguel, Malda, Jos, and Bottino, Marco C.

Published
2023

44. Composite Graded Melt Electrowritten Scaffolds for Regeneration of the Periodontal Ligament-to-Bone Interface

Author

Golafshan, Nasim, Castilho, Miguel, Daghrery, Arwa, Alehosseini, Morteza, van de Kemp, Tom, Krikonis, Konstantinos, de Ruijter, Mylene, Dal-Fabbro, Renan, Dolatshahi-Pirouz, Alireza, Bhaduri, Sarit B., Bottino, Marco C., Malda, Jos, Golafshan, Nasim, Castilho, Miguel, Daghrery, Arwa, Alehosseini, Morteza, van de Kemp, Tom, Krikonis, Konstantinos, de Ruijter, Mylene, Dal-Fabbro, Renan, Dolatshahi-Pirouz, Alireza, Bhaduri, Sarit B., Bottino, Marco C., and Malda, Jos

Abstract

Periodontitis is a ubiquitous chronic inflammatory, bacteria-triggered oral disease affecting the adult population. If left untreated, periodontitis can lead to severe tissue destruction, eventually resulting in tooth loss. Despite previous efforts in clinically managing the disease, therapeutic strategies are still lacking. Herein, melt electrowriting (MEW) is utilized to develop a compositionally and structurally tailored graded scaffold for regeneration of the periodontal ligament-to-bone interface. The composite scaffolds, consisting of fibers of polycaprolactone (PCL) and fibers of PCL-containing magnesium phosphate (MgP) were fabricated using MEW. To maximize the bond between bone (MgP) and ligament (PCL) regions, we evaluated two different fiber architectures in the interface area. These were a crosshatch pattern at a 0/90° angle and a random pattern. MgP fibrous scaffolds were able to promote in vitro bone formation even in culture media devoid of osteogenic supplements. Mechanical properties after MgP incorporation resulted in an increase of the elastic modulus and yield stress of the scaffolds, and fiber orientation in the interfacial zone affected the interfacial toughness. Composite graded MEW scaffolds enhanced bone fill when they were implanted in an in vivo periodontal fenestration defect model in rats. The presence of an interfacial zone allows coordinated regeneration of multitissues, as indicated by higher expression of bone, ligament, and cementoblastic markers compared to empty defects. Collectively, MEW-fabricated scaffolds having compositionally and structurally tailored zones exhibit a good mimicry of the periodontal complex, with excellent regenerative capacity and great potential as a defect-specific treatment strategy.

Published
2023

45. Composite Graded Melt Electrowritten Scaffolds for Regeneration of the Periodontal Ligament-to-Bone Interface

Author

Orthopaedie Onderzoek, Other research (not in main researchprogram), Regenerative Medicine and Stem Cells, Golafshan, Nasim, Castilho, Miguel, Daghrery, Arwa, Alehosseini, Morteza, van de Kemp, Tom, Krikonis, Konstantinos, de Ruijter, Mylene, Dal-Fabbro, Renan, Dolatshahi-Pirouz, Alireza, Bhaduri, Sarit B., Bottino, Marco C., Malda, Jos, Orthopaedie Onderzoek, Other research (not in main researchprogram), Regenerative Medicine and Stem Cells, Golafshan, Nasim, Castilho, Miguel, Daghrery, Arwa, Alehosseini, Morteza, van de Kemp, Tom, Krikonis, Konstantinos, de Ruijter, Mylene, Dal-Fabbro, Renan, Dolatshahi-Pirouz, Alireza, Bhaduri, Sarit B., Bottino, Marco C., and Malda, Jos

Published
2023

46. Tissue-specific melt electrowritten polymeric scaffolds for coordinated regeneration of soft and hard periodontal tissues

Author

Orthopaedie Onderzoek, Regenerative Medicine and Stem Cells, Other research (not in main researchprogram), Daghrery, Arwa, Ferreira, Jessica A., Xu, Jinping, Golafshan, Nasim, Kaigler, Darnell, Bhaduri, Sarit B., Malda, Jos, Castilho, Miguel, Bottino, Marco C., Orthopaedie Onderzoek, Regenerative Medicine and Stem Cells, Other research (not in main researchprogram), Daghrery, Arwa, Ferreira, Jessica A., Xu, Jinping, Golafshan, Nasim, Kaigler, Darnell, Bhaduri, Sarit B., Malda, Jos, Castilho, Miguel, and Bottino, Marco C.

Published
2023

47. Unveiling the Potential of Melt Electrowriting in Regenerative Dental Medicine

Author

Orthopaedie Onderzoek, Other research (not in main researchprogram), Regenerative Medicine and Stem Cells, Daghrery, Arwa, de Souza Araújo, Isaac J, Castilho, Miguel, Malda, Jos, Bottino, Marco C, Orthopaedie Onderzoek, Other research (not in main researchprogram), Regenerative Medicine and Stem Cells, Daghrery, Arwa, de Souza Araújo, Isaac J, Castilho, Miguel, Malda, Jos, and Bottino, Marco C

Published
2023

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