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The Nankai Trough Seismogenic Zone Experiment (NanTroSEIZE) is a multidisciplinary investigation of fault mechanics and seismogenesis along subduction megathrusts and includes reflection and refraction seismic imaging, direct sampling by drilling, in situ measurements, and long-term monitoring in conjunction with laboratory and numerical modeling studies. The fundamental objectives of NanTroSEIZE are to characterize the nature of fault slip and strain accumulation, fault and wall rock composition, fault architecture, and state variables throughout an active plate boundary system. As part of the NanTroSEIZE program, operations during Integrated Ocean Drilling Program (IODP) Expedition 348 were planned to extend and case riser Hole C0002F, begun during IODP Expedition 326 in 2010 and continued during Expedition 338 in 2012, from 860 to 3600 meters below the seafloor (mbsf). Riser operations during Expedition 348 were carried out and deepened the hole to 3058 mbsf, a new maximum depth record in scientific ocean drilling. Operations included installation and cementing of 13⅜ inch casing to 2008.9 mbsf and an 11¾ inch liner to 2922.5 mbsf, stabilizing the borehole for future deepening. Reaching this depth required two sidetracking operations from the original Hole C0002F, resulting in the designation of Holes C0002N and C0002P for the successively deeper sidetracks. During drilling, a suite of logging-while-drilling (LWD) and measurement-while-drilling (MWD), mud-gas, and cuttings data were collected over the interval from 2162.5 to 3058.5 mbsf in Hole C0002P, and a partial suite was collected in Hole C0002N. The interval from 2163 to 2218 mbsf was cored with the rotary core barrel (RCB). Planned future riser drilling operations will deepen the hole to penetrate the plate boundary fault at ~4600–5200 mbsf.

The deeper, inner parts of active accretionary prisms have been poorly studied due the lack of drilling data, low seismic image quality and typically thick overlying sediments. Our project focuses on the interior of the Nankai Trough inner accretionary prism using deep scientific drilling and a 3D seismic cube. International Ocean Discovery Program (IODP) Expedition 348 extended the existing riser hole to more than 3000 meters below seafloor (mbsf) at Site C0002. Logging while drilling (LWD) data included gamma ray, resistivity, resistivity image, and sonic logs. LWD analysis of the lower section revealed on the borehole images intense deformation characterized by steep bedding, faults and fractures. Bedding plane orientations were measured throughout, with minor gaps at heavily deformed zones disrupting the quality of the resistivity images. Bedding trends are predominantly steeply dipping (60-90°) to the NW. Interpretation of fractures and faults in the image log revealed the existence of different sets of fractures and faults and variable fracture density, remarkably high at fault zones. Gamma ray, resistivity and sonic logs indicated generally homogenous lithology interpretation along this section, consistent with the ¿silty-claystone¿ predominant lithologies described on cutting samples. Drops in sonic velocity were observed at the fault zones defined on borehole images. Seismic reflection interpretation of the deep faults in the inner prism is exceedingly difficult due to a strong seafloor multiple, high-angle bedding dips, and low frequency of the data. Structural reconstructions were employed to test whether folding of seismic horizons in the overlying forearc basin could be from an interpreted paleothrust within the inner prism. We used a trishear-based restoration to estimate fault slip on folded horizons landward of C0002. We estimate ~500 m of slip from a steeply dipping deep thrust within the last ~0.9 Ma. Folding is not found in the Kumano sediments near C0002, where normal faults and tilting dominate the modern basin deformation. Both logging and seismic are consistent in characterizing a heavily deformed inner prism. Most of this deformation must have occurred during or before formation of the overlying modern Kumano forearc basin sediments.

The International Ocean Discovery Program (IODP) Expedition 348 extended the borehole of Site C0002, Nankai Trough, down to over 3000 meters below sea floor (mbsf) collecting core samples and in-situ geophysical data from the inner accretionary prism. In order to investigate the in-situ stress state within the prism, we characterized the occurrence and shapes of wellbore failures as observed by resistivity image logs and sonic caliper logs collected in the vertical well of Hole C0002P. Most wellbore failures were observed in the top 70 meters of Hole C0002P (2150 to 2218.5 mbsf), where resistivity images were acquired several days after the borehole was initially exposed by previous drilling and coring runs. Wide breakouts spanning up to 140 degrees are observed in the NW/SE to NNW/SSE direction suggesting that the maximum horizontal principal stress in the cored interval is in the direction generally consistent with those observed in the shallower sections of Site C0002 from earlier expeditions. In the remaining section of the borehole below, wellbore failures are much sparse and subtle in the resistivity image possibly due to the short exposure time between drilling and image acquisition (about 2 hours). Preliminary examination suggest that these features are breakouts aligned in the direction consistent with the upper section, but examination in combination with the sonic caliper data is required for further confirmation. These observations suggest that the occurrence of breakouts exhibit significant time-dependence due to processes such as pore pressure diffusion or time-dependent rock deformation. Moreover, sonic caliper data suggests that shapes and width of the wellbore failures in the studied dataset is influenced by the formation strength anisotropy (in the horizontal direction) enhanced by the steeply dipping bedding planes (60-90 degrees). Thus, constraints on in-situ stress magnitudes will be provided through comparison of wellbore failures, borehole cross section, bedding dips, downhole mud pressure records, and formation mechanical properties

Molecular cloning experiments have led to the identification and characterization of a family of five receptors for the melanocortin (melanotropic and adrenocorticotropic) peptides. The first two members of the family cloned were the well-characterized melanocyte-stimulating hormone receptor (MSH-R) and adrenocorticotropin receptor (ACTH-R). The three new melanocortin receptors have been termed the MC3-R, MC4-R, and MC5-R, according to the order of their discovery, and little is known at this point concerning their function. Agouti and extension are two genetic loci known to control the amounts of eumelanin (brown-black) and phaeomelanin (yellow-red) pigments. Chromosomal mapping demonstrated that the MSH-R, now termed MCI-R, mapped to extension. Extension was shown to encode the MCI-R, and mutations in the MCI-R are responsible for the different pigmentation phenotypes caused by this locus. Functional variants of the MCI-R, originally characterized in the mouse, have now also been identified in the guinea pig and cow. Dominant constitutive mutants of the MCI-R are responsible for causing dark black coat colors while recessive alleles result in yellow or red coat colors. Agouti, a secreted 108 amino acid peptide produced within the hair follicle, acts on follicular melanocytes to inhibit alpha-MSH-induced eumelanin production. Experiments demonstrate that agouti is a high-affinity antagonist, acting at the MCI-R to block alpha-MSH stimulation of adenylyl cyclase, the effector through which alpha-MSH induces eumelanin synthesis. The MCI-R is thus a unique bifunctionally controlled receptor, activated by alpha-MSH and antagonized by agouti, both contributing to the variability seen in mammalian coat colors. The variable tan and black coat color patterns seen in the German Shepherd, for example, can now be understood on the molecular level as the interaction of a number of extension and agouti alleles encoding variably functioning receptors and a differentially expressed antagonist of the receptor, respectively.