152 results on '"Bostik, P."'
Search Results
2. Multicentric evaluation of sensitivity of eight commercial anti-SARS-CoV-2 antibody assays and their correlation to virus neutralization titers in seropositive subjects
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Miroslav Fajfr, Petr Pajer, Daniel Ruzek, Radek Sleha, Sylva Janovska, Milos Bohonek, Hana Kabickova, Pavla Kubicková, Michal Stefanik, Petra Strakova, and Pavel Bostik
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Medicine ,Science - Abstract
Abstract Diagnosis of SARS-CoV-2 virus is mainly based on direct detection. Determination of specific antibodies has been used mostly for epidemiological reasons. However, select immunoassays showed good correlation to plaque reduction virus neutralization test (PRNT) in smaller patient cohorts, which suggests their potential as predictors of virus neutralization titer. A total of 3,699 samples from Covid-19 patients were included in the multicentric study performed in the Czech Republic. Anti-SARS-CoV-2 antibody levels were evaluated by 8 commercial antibody assays. Simultaneously, PRNT evaluations were performed with the SARS-CoV-2 B.1.258 variant. All immunoassays showed an overall high true positive diagnostic value ranging from 79.17 to 98.04%. Several commercial EIA methods showed highly positive correlation between the assay results and PRNT levels, e.g., Liaison CoV-2 TrimericS IgG DiaSorin (Spearman r = 0.8833; Architect SASRS-CoV-2 IgG Abbott (r = 0.7298); NovaLisa SARS-CoV-2 IgG NovaTec (r = 0.7103) and Anti-SARS-CoV-2 ELISA IgG Euroimmun (r = 0.7094). While this correlation was less positive for other assays, those, conversely, presented higher true positive values. For most immunoassays, the positive percent agreement of the results was ≥ 95% in sera exhibiting PRNT levels of 1:80 and higher. The assays tested have shown variable correlation to PRNT. Those possessing high positive predictive values serve well as qualitative tests, while others can be utilised as quantitative tests highly predictive of neutralization antibody levels.
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- 2024
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3. Visual diagnosis of the Varroa destructor parasitic mite in honeybees using object detector techniques
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Bilik, Simon, Kratochvila, Lukas, Ligocki, Adam, Bostik, Ondrej, Zemcik, Tomas, Hybl, Matous, Horak, Karel, and Zalud, Ludek
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Computer Science - Computer Vision and Pattern Recognition ,Computer Science - Artificial Intelligence ,Computer Science - Machine Learning - Abstract
The Varroa destructor mite is one of the most dangerous Honey Bee (Apis mellifera) parasites worldwide and the bee colonies have to be regularly monitored in order to control its spread. Here we present an object detector based method for health state monitoring of bee colonies. This method has the potential for online measurement and processing. In our experiment, we compare the YOLO and SSD object detectors along with the Deep SVDD anomaly detector. Based on the custom dataset with 600 ground-truth images of healthy and infected bees in various scenes, the detectors reached a high F1 score up to 0.874 in the infected bee detection and up to 0.727 in the detection of the Varroa Destructor mite itself. The results demonstrate the potential of this approach, which will be later used in the real-time computer vision based honey bee inspection system. To the best of our knowledge, this study is the first one using object detectors for this purpose. We expect that performance of those object detectors will enable us to inspect the health status of the honey bee colonies.
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- 2021
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4. Czech Honeydew Honeys—A Potential Source of Local Medical Honey with Strong Antimicrobial Activity
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Ludovit Pudelka, Radek Sleha, Sylva Janovska, Vera Radochova, and Pavel Bostik
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honeydew ,antibacterial effect ,hydrogen peroxide ,polyphenolic compounds ,Medicine ,Pharmacy and materia medica ,RS1-441 - Abstract
An increasing resistance of microbes to antibiotics, the emergence of multidrug-resistant and extremely resistant strains, and the long time needed to develop new antibiotics are driving the search for additional sources of antibacterial agents. The aim of the study was to compare the efficacy of Czech honeys with already available pharmaceutical agents containing medicinal honey, and to perform basic biochemical analysis of Czech samples, including detection of undesirable chemical substances. The results showed strong antibacterial activity of Czech honeydew honeys compared to the control group, especially against G+ pathogens, with an average MIC of 9.44% compared to 17.54%, and comparable activity against G− of 16.48% versus 16.66%. In addition to the strong antibacterial activity, this study confirmed the safety and quality of Czech honeys and helped to select the character of a possible source for in vivo testing and subsequent clinical trials.
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- 2024
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5. Modulation of Gut Microbiome and Autism Symptoms of ASD Children Supplemented with Biological Response Modifier: A Randomized, Double-Blinded, Placebo-Controlled Pilot Study
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Jela Hrnciarova, Klara Kubelkova, Vanda Bostik, Ivan Rychlik, Daniela Karasova, Vladimir Babak, Magdalena Datkova, Katerina Simackova, and Ales Macela
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autism ,microbiome ,biological response modifier ,psychobiotics ,Nutrition. Foods and food supply ,TX341-641 - Abstract
The etiology and mechanisms of autism and autism spectrum disorder (ASD) are not yet fully understood. There is currently no treatment for ASD for providing significant improvement in core symptoms. Recent studies suggest, however, that ASD is associated with gut dysbiosis, indicating that modulation of gut microbiota in children with ASD may thus reduce the manifestation of ASD symptoms. The aim of this pilot study (prospective randomized, double-blinded, placebo-controlled) was to evaluate efficacy of the biological response modifier Juvenil in modulating the microbiome of children with ASD and, in particular, whether Juvenil is able to alleviate the symptoms of ASD. In total, 20 children with ASD and 12 neurotypical children were included in our study. Supplementation of ASD children lasted for three months. To confirm Juvenil’s impact on the gut microbiome, stool samples were collected from all children and the microbiome’s composition was analyzed. This pilot study demonstrated that the gut microbiome of ASD children differed significantly from that of healthy controls and was converted by Juvenil supplementation toward a more neurotypical microbiome that positively modulated children’s autism symptoms.
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- 2024
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6. The sensitivity and specificity of Abbott Panbio™ COVID 19 Ag Rapid test in the context of four SARS-CoV-2 variants
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Miroslav Fajfr, Laith Moolla, Joudi Barout, Saaz Sahani, Rudolf Kukla, Eva Cermakova, Radek Sleha, and Pavel Bostik
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SARS CoV-2 variant ,Rapid antigen test ,PCR ct value ,Sensitivity ,Specificity ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Rapid antigen tests for the detection of SARS-CoV-2 are commonly used for the diagnosis of Covid-19. Previously published data showed a wide range of sensitivity and specificity of RATs, but these studies were performed on relatively small numbers of samples and using only limited numbers of virus variants. The aim of the study was to evaluate the main parameters of a commonly used RAT for 4 different virus variants in comparison with PCR. Material and methods: A set of 2874 samples obtained from Covid-19 patients were examined both by PCR and RAT. Two commercial PCR kits (Generi Biotech, Diana Biotechnologies) and one RAT – Abbott Panbio™ COVID 19 Ag Rapid – were compared for their sensitivity and specificity in samples positive for one of the four different SARS-CoV-2 variants – B.1.258 (n = 496), Alpha (n = 645), Delta/Delta+ (n = 687), and Omicron (n = 1046). Results: The sensitivity of Panbio™ COVID19 Ag Rapid test varied from 80.0 % in Omicron to 88.92 % in Alpha variants. The specificities of the RAT for all variants reached above 93 %. Statistically significant differences were found between the results from RAT assay in select virus variants. In addition, significantly higher sensitivity (p
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- 2024
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7. Long-Term Immunological Alertness and Response to COVID-19 Vaccination—Conditions for Prevention in Early Palliative Oncological Care Patients
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Peter Priester, Miroslav Fajfr, Veronika Molnarova, Radek Sleha, Sylva Janovska, Pavel Bostik, and Stanislav Filip
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palliative oncological care ,COVID-19 vaccine ,anti-S IgG ,VNT ,inpatient care ,Medicine - Abstract
Aside from the general population, the COVID-19 pandemic has also affected a group of patients in palliative oncology care. In this study, long-term immune responses against SARS-CoV-2 after vaccination were monitored in a cohort of patients in palliative oncology care. This non-randomized, prospective, and open-label pilot study recruited patients from the Palliative Oncology Program and included 147 patients, of which 80 were females (54.4%) and 67 males (45.6%). The overall evaluation included current health status, SARS-CoV-2 anti-S IgG titer, and neutralizing antibodies using the SARS-CoV-2 virus neutralization test (VNT). Anti-S IgG antibody analysis revealed high (H) antibody levels in 35.7% (n = 10) and very high (VH) levels in 39.3% (n = 11) of patients after the second vaccination dose. Similarly, after the third dose, H was found in 29.6% (n = 32) and VH in 55.5% (n = 60) of patients. High and very high anti-S IgG antibody levels were consistent with high VNT titers (>2560) and H antibody levels in 17.1% (n = 12) or VH in 82.9% (n = 58) of patients. Patients with two or more doses showed H and VH antibody levels at a median of 451 and 342 days after vaccination, respectively. In this clinical trial, patients showed high and very high levels of anti-S IgG antibodies over a longer period of time. These patients did not show reduced immunological responses to the COVID-19 vaccine challenge. We can assume that prevention through vaccination can reduce the risk of complications or death from COVID-19 in patients in early palliative oncology care.
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- 2024
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8. Clinical characteristics of colonization of the amniotic cavity in women with preterm prelabor rupture of membranes, a retrospective study
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Kacerovsky, Marian, Stranik, Jaroslav, Matulova, Jana, Chalupska, Martina, Mls, Jan, Faist, Tomáš, Hornychova, Helena, Kukla, Rudolf, Bolehovska, Radka, Bostik, Pavel, Jacobsson, Bo, and Musilova, Ivana
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- 2022
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9. Clinical characteristics of colonization of the amniotic cavity in women with preterm prelabor rupture of membranes, a retrospective study
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Marian Kacerovsky, Jaroslav Stranik, Jana Matulova, Martina Chalupska, Jan Mls, Tomáš Faist, Helena Hornychova, Rudolf Kukla, Radka Bolehovska, Pavel Bostik, Bo Jacobsson, and Ivana Musilova
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Medicine ,Science - Abstract
Abstract To determine the main clinical characteristics of preterm prelabor rupture of membranes (PPROM) complicated by colonization of the amniotic cavity (microbial invasion of the amniotic cavity without intra-amniotic inflammation). A total of 302 women with PPROM were included. Transabdominal amniocentesis was performed and amniotic fluid was assessed. Based of microbial invasion of the amniotic cavity and intra-amniotic inflammation (interleukin-6 ≥ 3000 pg/mL), the women were divided into following groups: intra-amniotic infection, sterile intra-amniotic inflammation, colonization of the amniotic cavity, and negative amniotic fluid. Colonization was found in 11% (32/302) of the women. The most common bacteria identified in the amniotic fluid were Ureaplasma spp. with a lower burden than those with intra-amniotic infection (p = 0.03). The intensity of intra-amniotic inflammatory response measured by interleukin-6 was higher in women with colonization than in those with negative amniotic fluid (medians: 961 pg/mL vs. 616 pg/mL; p = 0.04). Women with colonization had higher rates of acute inflammatory placental lesions than those with negative amniotic fluid. In PPROM, colonization, caused mainly by microorganisms from the lower genital tract, might represent an early stage of microbial invasion of the amniotic cavity with a weak intra-amniotic inflammatory response.
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- 2022
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10. Intra-amniotic inflammation and birth weight in pregnancies with preterm labor with intact membranes: A retrospective cohort study
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Jana Matulova, Marian Kacerovsky, Radka Bolehovska, Rudolf Kukla, Pavel Bostik, Klara Kolarova, Simona Frydrychová, Bo Jacobsson, and Ivana Musilova
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amniocentesis ,amniotic fluid ,estimated fetal weight ,fetal growth ,intergrowth ,intra-amniotic inflammation ,Pediatrics ,RJ1-570 - Abstract
ObjectiveTo assess the association between newborn birth weight and the presence of intra-amniotic infection, presence of sterile intra-amniotic inflammation, and absence of intra-amniotic inflammation in pregnancies with preterm labor with intact membranes.MethodsA total of 69 pregnancies with preterm labor with intact membranes between gestational ages 22 + 0 and 34 + 6 weeks who delivered within seven days of admission were included in this retrospective cohort study. Transabdominal amniocentesis to determine the presence of microorganisms and/or their nucleic acids in amniotic fluid (through culturing and molecular biology methods) and intra-amniotic inflammation (according to amniotic fluid interleukin-6 concentrations) were performed as part of standard clinical management. The participants were further divided into three subgroups: intra-amniotic infection (presence of microorganisms and/or nucleic acids along with intra-amniotic inflammation), sterile intra-amniotic inflammation (intra-amniotic inflammation alone), and without intra-amniotic inflammation. Birth weights of newborns were expressed as percentiles derived from the INTERGROWTH-21st standards for (i) estimated fetal weight and (ii) newborn birth weight.ResultsNo difference in birth weights, expressed as percentiles derived from the standard for estimated fetal weight, was found among the women with intra-amniotic infection, with sterile intra-amniotic inflammation, and without intra-amniotic inflammation (with infection, median 29; with sterile inflammation, median 54; without inflammation, median 53; p = 0.06). Differences among the subgroups were identified in the birth weight rates, expressed as percentiles derived from the standard for estimated fetal weight, which were less than the 10th percentile (with infection: 20%, with inflammation: 13%, without inflammation: 0%; p = 0.04) and 25th percentile (with infection: 47%, with inflammation: 31%, without inflammation: 9%; p = 0.01). No differences among the subgroups were observed when percentiles of birth weight were derived from the birth weight standard.ConclusionsThe presence of intra-amniotic inflammatory complications in pregnancies with preterm labor with intact membranes prior to the gestational age of 35 weeks was associated with a higher rate of newborns with birth weight less than the 10th and 25th percentile, when percentiles of birth weight were derived from the standard for estimated fetal weight.
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- 2022
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11. Semi-supervised deep learning approach to break common CAPTCHAs
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Bostik, Ondrej, Horak, Karel, Kratochvila, Lukas, Zemcik, Tomas, and Bilik, Simon
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- 2021
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12. Heterogeneity of post-COVID impairment: interim analysis of a prospective study from Czechia
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Mikulas Skala, Michal Svoboda, Michal Kopecky, Eva Kocova, Martin Hyrsl, Michal Homolac, Viktor Chrobok, Pavel Bostik, Miroslav Fajfr, Petr Prasil, Stanislav Plisek, Radek Sleha, and Vladimir Koblizek
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Post-COVID ,Symptom ,Lung function ,6-min Walk Test ,Computer tomography ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract We stratified post-COVID patients into four newly established clinical groups based on the presence or absence of at least one subjective respiratory symptom and at least one objective sign of pulmonary involvement. Nearly half of outpatients and one third of hospitalized post-COVID patients had objective signs of pulmonary involvement without accompanying subjective respiratory symptoms three months after diagnosis.
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- 2021
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13. IgGFc-binding protein in pregnancies complicated by spontaneous preterm delivery: a retrospective cohort study
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Jaroslav Stranik, Marian Kacerovsky, Ondrej Soucek, Martina Kolackova, Ivana Musilova, Lenka Pliskova, Radka Bolehovska, Pavel Bostik, Jana Matulova, Bo Jacobsson, and Ctirad Andrys
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Medicine ,Science - Abstract
Abstract To determine the IgGFc-binding protein (FcgammaBP) concentration in amniotic and cervical fluids in preterm prelabor rupture of membranes (PPROM) and preterm labor with intact membranes (PTL) and to assess the diagnostic indices of FcgammaBP to predict intra-amniotic infection (the presence of both microbial invasion of the amniotic cavity and intra-amniotic inflammation). In this study, we included 170 and 79 women with PPROM and PTL, respectively. Paired cervical and amniotic fluid samples were obtained using a Dacron polyester swab and transabdominal amniocentesis, respectively. The FcgammaBP concentrations in the samples were assessed using an enzyme-linked immunosorbent assay. The presence of intra-amniotic infection was associated with elevated FcgammaBP concentrations in pregnancies with PPROM and PTL [PPROM—presence: 86 ng/mL vs. absence: 13 ng/mL, p
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- 2021
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14. Innate Immune Recognition, Integrated Stress Response, Infection, and Tumorigenesis
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Klara Kubelkova, Vanda Bostik, Lokesh Joshi, and Ales Macela
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innate immunity ,infection ,tumorigenesis ,Biology (General) ,QH301-705.5 - Abstract
Engagement of PRRs in recognition of PAMPs or DAMPs is one of the processes that initiates cellular stress. These sensors are involved in signaling pathways leading to induction of innate immune processes. Signaling initiated by PRRs is associated with the activation of MyD88-dependent signaling pathways and myddosome formation. MyD88 downstream signaling depends upon the context of signaling initiation, the cell (sub)type and the microenvironment of signal initiation. Recognition of PAMPs or DAMPs through PRRs activates the cellular autonomous defence mechanism, which orchestrates the cell responses to resolve specific insults at the single cell level. In general, stressed endoplasmic reticulum is directly linked with the induction of autophagy and initiation of mitochondrial stress. These processes are regulated by the release of Ca2+ from ER stores accepted by mitochondria, which respond through membrane depolarization and the production of reactive oxygen species generating signals leading to inflammasome activation. In parallel, signaling from PRRs initiates the accumulation of misfolded or inappropriately post-translationally modified proteins in the ER and triggers a group of conserved emergency rescue pathways known as unfolded protein response. The cell-autonomous effector mechanisms have evolutionarily ancient roots and were gradually specialized for the defence of specific cell (sub)types. All of these processes are common to the innate immune recognition of microbial pathogens and tumorigenesis as well. PRRs are active in both cases. Downstream are activated signaling pathways initiated by myddosomes, translated by the cellular autonomous defence mechanism, and finalized by inflammasomes.
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- 2023
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15. Prevalence and Load of Cervical Ureaplasma Species With Respect to Intra-amniotic Complications in Women With Preterm Prelabor Rupture of Membranes Before 34 weeks
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Marian Kacerovsky, Rudolf Kukla, Radka Bolehovska, Pavel Bostik, Jana Matulova, Jan Mls, Jaroslav Stranik, Bo Jacobsson, and Ivana Musilova
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microbial invasion of the amniotic cavity ,genital mycoplasma ,intra-amniotic inflammation ,non-invasive sample ,preterm delivery ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Objectives: To determine the prevalence and load of Ureaplasma spp. DNA in the cervical fluid of women with singleton pregnancies complicated by preterm prelabor rupture of membranes (PPROM) with respect to intra-amniotic infection, sterile intra-amniotic inflammation, and colonization of the amniotic fluid.Methods: A total of 217 women with PPROM between gestational ages 24 + 0 and 33 + 6 weeks were included in this study. Paired amniotic and cervical fluid samples were collected at the time of admission via transabdominal amniocentesis and using a Dacron polyester swab, respectively. Microbial invasion of the amniotic cavity was diagnosed using a combination of culture and molecular biology methods. Intra-amniotic inflammation was determined based on the concentration of interleukin-6 in the amniotic fluid. Based on the presence or absence of these conditions, the women were stratified into the following subgroups: intra-amniotic infection (with both), sterile intra-amniotic inflammation (with inflammation only), colonization (with microorganisms only), and negative amniotic fluid (without either). The Ureaplasma spp. DNA load in the cervical fluid was assessed using PCR.Results:Ureaplasma spp. DNA in the cervical fluid was found in 61% (133/217) of the women. Women with negative amniotic had similar prevalence of Ureaplasma spp. DNA in cervical fluid (55%) to those with sterile intra-amniotic inflammation (54%) but lower than those with intra-amniotic infection (73%) and colonization (86%; p < 0.0001). Women with negative amniotic fluid had a lower load of Ureaplasma spp. DNA in their cervical fluid (median: 4.7 × 103 copies of DNA/ml) than those with intra-amniotic infection (median: 2.8 × 105 copies DNA/ml), sterile intra-amniotic inflammation (median: 5.3 × 104 copies DNA/ml), and colonization (median: 1.2 × 105 copies DNA/mL; p < 0.0001).Conclusion: In conclusion, in PPROM at
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- 2022
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16. A rare case of purulent meningitis caused by Capnocytophaga canimorsus in the Czech Republic – case report and review of the literature
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Petr Prasil, Lenka Ryskova, Stanislav Plisek, and Pavel Bostik
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Capnocytophaga ,Purulent meningitis ,Invasive infection ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Invasive infections caused by Capnocytophaga canimorsus are rare. Immunocompromised patients, who report being bitten by or having a close contact with an animal, represent a high-risk group for this infection. There are only few dozens of infections by this bacteria manifesting as purulent meningitis reported worldwide. The reported case is a first reported case of purulent meningitis caused by by Capnocytophaga canimorsus in Czech Republic with only a limited risk factor history. Case presentation The patient, a 74 years old man, was referred to the infectious diseases department of a teaching hospital with clear signs of developing purulent meningitis. His anamnestic data did not show any unusual findings. He was treated for compensated diabetes mellitus type II. The blood cultures were negative and the etiological agent did not grow from the cerebrospinal fluid (CSF) on common media. Eventually, it was identified by detecting pan-bacterial DNA and DNA sequencing. Subsequently, the pathogen was confirmed by anaerobic cultivation from CSF. Only after then the patient recalled being bitten by his German shepherd puppy during play. The patient was successfully treated intravenously by ceftriaxone. Conclusions Purulent meningitis caused by Capnocytophaga spp. is a rare disease, but it needs to be considered in patients at risk with pre-existing conditions, who report close contact with or being bitten by an animal. It is important to test for this microbe in cases with negative microbiological results for the more common agents.
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- 2020
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17. A Rare Case of Osteomyelitis of an Ankle Caused by Mycobacterium chelonae
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Lenka Ryskova, Rudolf Kukla, Radka Bolehovska, Libor Prokes, Milan Vajda, Tomas Kucera, Ivo Pavlik, Pavel Bostik, and Pavel Ryska
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nontuberculous potentially pathogenic mycobacteria ,rapidly growing mycobacteria ,osteomyelitis ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Mycobacterium chelonae, a rapidly growing nontuberculous mycobacterium, is usually described as a causative agent of soft tissue infections (postsurgical, posttraumatic, posttransplantation, postinjection, catheter infection, etc.), but only rarely as a cause of osteomyelitis. The authors describe a case report of a 72-year-old man with osteomyelitis of the talus. Initially, the infection was assessed as a soft tissue infection, without any osteolytic changes on the X-ray. After cultivation with subsequent targeted molecular typing of the rpoB gene, M. chelonae was identified from the affected tissue. The bone involvement was subsequently detected on MRI and confirmed histologically with findings of the granulomatous tissue and acid-fast bacilli. The patient was initially treated intravenously with a combination of tigecycline, amikacin, and moxifloxacin for 4 weeks, after which the oral combination of doxycycline and moxifloxacin continued. Identification of the infecting pathogen using molecular typing thus helped to establish the correct diagnosis and represents a rarely described case of osteomyelitis caused by M. chelonae.
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- 2023
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18. IgGFc-binding protein in pregnancies complicated by spontaneous preterm delivery: a retrospective cohort study
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Stranik, Jaroslav, Kacerovsky, Marian, Soucek, Ondrej, Kolackova, Martina, Musilova, Ivana, Pliskova, Lenka, Bolehovska, Radka, Bostik, Pavel, Matulova, Jana, Jacobsson, Bo, and Andrys, Ctirad
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- 2021
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19. Heterogeneity of post-COVID impairment: interim analysis of a prospective study from Czechia
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Skala, Mikulas, Svoboda, Michal, Kopecky, Michal, Kocova, Eva, Hyrsl, Martin, Homolac, Michal, Chrobok, Viktor, Bostik, Pavel, Fajfr, Miroslav, Prasil, Petr, Plisek, Stanislav, Sleha, Radek, and Koblizek, Vladimir
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- 2021
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20. Dual Infection of an Open Fracture Caused by Mycobacterium setense and Clostridium celerecrescens
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Lenka Ryskova, Jan Zahradnicek, Rudolf Kukla, Radka Bolehovska, Milan Vajda, Ivo Pavlik, Pavel Bostik, and Pavel Ryska
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nontuberculous mycobacteria ,rapidly growing mycobacteria ,post-traumatic osteomyelitis ,fracture-related infection ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Infections caused by Mycobacterium setense or Clostridium celerecrescens are extremely rare. In this report, for the first time a dual infection with these two pathogens is described. An 18-year-old female suffered multiple injuries, including an open comminuted fracture of the right humeral diaphysis after falling from a fifth-floor balcony in January 2019. Five months after the accident, a fistula appeared in the scar, reaching the bone tissue. M. setense and C. celerecrescens were cultured from sinus swabs and subsequently from perioperative samples. The patient was initially treated with a combination of intravenous antibiotics (ATBs): imipenem, amikacin, and ciprofloxacin. One month after the fracture fixation with a titanium nail, C. celerecrescens was again detected; therefore, metronidazole was added to the therapy. A triple combination of oral (PO) ATBs (trimethoprim–sulfamethoxazole, moxifloxacin, and metronidazole) followed, 8 weeks after the initial intravenous therapy. C. celerecrescens was cultured again two times, most recently in November 2019, when surgical debridement was supplemented by the topical administration of cancellous bone impregnated with vancomycin. Signs of bone healing were found at follow-ups and ATB treatment was finished in March 2020 after a total of 9 months of therapy. To this day, there have been no signs of reinfection. This case thus illustrates the need for a combination of systemic and individualized local therapy in the treatment of complicated cases of dual infections with rare pathogens.
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- 2022
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21. Mycobacterioses Induced by Mycobacterium abscessus: Case Studies Indicating the Importance of Molecular Analysis for the Identification of Antibiotic Resistance
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Lenka Ryskova, Radka Bolehovska, Rudolf Kukla, Michal Svarc, Alzbeta Zavrelova, Hubert Vanicek, Ivo Pavlik, and Pavel Bostik
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nontuberculous mycobacteria ,rapidly growing mycobacteria ,macrolide resistance ,erm(41) gene ,multiresistance ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Mycobacterioses are less frequently occurring but serious diseases. In recent years, at a global level, the incidence of mycobacterioses induced by the rapidly growing species Mycobacterium abscessus (M. a.), which is considered to be the most resistant to antibiotics and most difficult to treat, has been on the rise. Correct identification to the level of the subspecies (M. a. abscessus, M. a. massiliense, and M. a. bolletii) and determination of its sensitivity to macrolides, which are the basis of combination therapy, are of principal importance for the management of the disease. We describe five cases of mycobacterioses caused by M. a., where the sequencing of select genes was performed to identify the individual subspecies and antibiotic resistance. The analysis of the rpoB gene showed two isolates each of M. a. abscessus and M. a. massiliense and one isolate of M. a. bolletii. The complete (full length) erm(41) gene responsible for the development of inducible resistance to macrolides was demonstrated in both M. a. abscessus and M. a. bolletii isolates. A partially deleted and non-functional erm(41) gene was demonstrated in M. a. massiliense isolates. The subsequent sequencing of the full length erm(41) gene products showed, however, the mutation (T28→C) in both isolates of M. a. abscessus, causing a loss of the function and preserved sensitivity to macrolides. The antibiotic sensitivity testing confirmed that both the isolates of M. a. abscessus and M. a. massiliense were sensitive to clarithromycin even after prolonged 14-day incubation. The inducible resistance to clarithromycin was maintained only in M. a. bolletii. Thus, the sequence analysis of the erm(41) gene can reliably identify the preservation of sensitivity to macrolides and serve as an important tool in the establishment of therapeutic regimens in cases of infections with M. abscessus.
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- 2022
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22. Inhibitory effect of hop fractions against Gram-positive multi-resistant bacteria. A pilot study
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Katerina Bogdanova, Milan Kolar, Katerina Langova, Martin Dusek, Alexandr Mikyska, Vanda Bostikova, Pavel Bostik, and Jana Olsovska
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humulone ,lupulone ,xanthohumol ,humulus lupulus l. ,antimicrobial properties ,multi-resistant bacteria ,Medicine - Abstract
Aim: Our research focused on the antimicrobial effects of purified hop (Humulus lupulus L.) fractions including α-bitter acids (humulones), β-bitter acids (lupulones) and xanthohumol, and a commercial CO2 hop extract of bitter acids against reference and multi-resistant strains of Gram-positive and Gram-negative bacteria and against selected yeast strains. Methods: In vitro testing of antimicrobial activity was performed according to standard testing protocols (EUCAST). The effects of hop extracts on bacterial/yeast strains at concentrations below MICs were also determined and the antimicrobial potential of hop extracts was compared with selected antibiotics using optical density measurement. Results: The fractions were effective not only against reference strains of Gram-positive bacteria but, more importantly, against their methicillin- and vancomycin-resistant variants. No antimicrobial effect was detected against Gram-negative bacterial strains. Among the tested substances, xanthohumol was identified as the hop fraction with the most potent antimicrobial properties. It was also found that hop substances exerted their antimicrobial effects at concentrations considerably lower than the determined MICs, with the strongest effect in case of α-bitter acids in enterococci. Conclusion: The search for and research of new compounds with antimicrobial properties represents a possible solution to the current global problem of bacterial resistance. Our data suggest a desirable activity of hop fractions against some multi-resistant bacterial strains. Thus, hops might find use as a source of potential antimicrobial agents applicable in both human and veterinary medicine.
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- 2018
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23. Long-Term Antibody Response and Vaccination Efficacy in Patients with COVID-19: A Single Center One-Year Prospective Study from the Czech Republic
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Miroslav Fajfr, Radek Sleha, Sylva Janovska, Vladimir Koblizek, Mikulas Skala, Stanislav Plisek, Petr Prasil, Petr Smahel, and Pavel Bostik
- Subjects
serology ,SARS-CoV-2 ,virus neutralization test ,ELISA ,dynamics ,vaccination ,Microbiology ,QR1-502 - Abstract
Background: The diagnosis of SARS-CoV-2 is almost exclusively performed by PCR or antigen detection. The detection of specific antibodies has not yet been considered in official diagnostic guidelines as major laboratory evidence for a case definition. The aim the present study is to analyze antibody responses in outpatient and inpatient cohorts of COVID-19 patients in the Czech Republic over a 12-month period, and assess the potential of antibodies as a diagnostic tool. Methods: A total of 644 patients was enrolled in the prospective study. IgA, IgM and IgG antibody levels, as well as virus neutralization titers, were analyzed over a 12-month period. Results: Our study showed low antibody positivity levels at the admission. However, at 2 weeks after infection, 98.75% and 95.00% of hospitalized patients were IgA and IgG positive, respectively. Even in the outpatient cohort characterized by milder disease courses, the IgG antibody response was still sustained at 9 and 12 months. The data show a high correlation between the IgG levels and virus neutralization titers (VNTs). Samples from later time-points showed positive antibody responses after vaccination in both cohorts characterized by high IgG levels and VNT over 1:640. The samples from unvaccinated persons indicated a relatively high level of reinfection at 6.87%. Conclusions: Our results show that the detection of antibodies against the SARS-CoV-2 shows an increasing sensitivity from week 2 after infection and remains highly positive over the 12-month period. The levels of IgG antibodies correlate significantly with the VNTs. This suggests that the serological data may be a valuable tool in the diagnosis of SARS-CoV-2 infection.
- Published
- 2022
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24. A rare case of purulent meningitis caused by Capnocytophaga canimorsus in the Czech Republic – case report and review of the literature
- Author
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Prasil, Petr, Ryskova, Lenka, Plisek, Stanislav, and Bostik, Pavel
- Published
- 2020
- Full Text
- View/download PDF
25. Strong Antimicrobial and Healing Effects of Beta-Acids from Hops in Methicillin-Resistant Staphylococcus aureus-Infected External Wounds In Vivo
- Author
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Radek Sleha, Vera Radochova, Jiri Malis, Alexander Mikyska, Milan Houska, Karel Krofta, Katerina Bogdanova, Sylva Janovska, Jaroslav Pejchal, Milan Kolar, Pavel Cermak, and Pavel Bostik
- Subjects
hops ,methicillin-resistant ,Staphylococcus aureus ,infection ,porcine model ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Staphylococcus (S.) aureus is an important causative agent of wound infections with increasing incidence in the past decades. Specifically, the emergence of methicillin-resistant S. aureus (MRSA) causes serious problems, especially in nosocomial infections. Therefore, there is an urgent need to develop of alternative or supportive antimicrobial therapeutic modalities to meet these challenges. Purified compounds from hops have previously shown promising antimicrobial effects against MRSA isolates in vitro. In this study, purified beta-acids from hops were tested for their potential antimicrobial and healing properties using a porcine model of wounds infected by MRSA. The results show highly significant antimicrobial effects of the active substance in both the powder and Ambiderman-based application forms compared to both no-treatment control and treatment with Framycoin. Moreover, the macroscopic evaluation of the wounds during the treatment using the standardized Wound Healing Continuum indicated positive effects of the beta-acids on the overall wound healing. This is further supported by the microscopic data, which showed a clear improvement of the inflammatory parameters in the wounds treated by beta-acids. Thus, using the porcine model, we demonstrate significant therapeutic effects of hops compounds in the management of wounds infected by MRSA. Beta-acids from hops, therefore, represent a suitable candidate for the treatment of non-responsive nosocomial tissue infections by MRSA.
- Published
- 2021
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- View/download PDF
26. Segmentation Method Overview for Thermal Images in Matlab Computational Environment
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Ondrej Bostik, Sobeslav Valach, Karel Horak, and Jan Klecka
- Subjects
MATLAB ,segmentation ,thermal images ,dataset ,Otsu's segmentation ,adaptive thresholding ,Electronic computers. Computer science ,QA75.5-76.95 - Abstract
This paper presents an overview of methods usable for segmentation of thermal images in MATLAB computational environment. The goal of this work is the demonstration usage of available methods and evaluate their performance. Part of the work is to present the datasets we create for testing. This paper is part of our ongoing work focused on segmentation of thermal images from the process of traverse wedge rolling.
- Published
- 2019
- Full Text
- View/download PDF
27. Strong Antimicrobial Effects of Xanthohumol and Beta-Acids from Hops against Clostridioides difficile Infection In Vivo
- Author
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Radek Sleha, Vera Radochova, Alexander Mikyska, Milan Houska, Radka Bolehovska, Sylva Janovska, Jaroslav Pejchal, Lubica Muckova, Pavel Cermak, and Pavel Bostik
- Subjects
hops ,C. difficile ,infection ,rat model ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Clostridioides (C.) difficile is an important causative pathogen of nosocomial gastrointestinal infections in humans with an increasing incidence, morbidity, and mortality. The available treatment options against this pathogen are limited. The standard antibiotics are expensive, can promote emerging resistance, and the recurrence rate of the infection is high. Therefore, there is an urgent need for new approaches to meet these challenges. One of the possible treatment alternatives is to use compounds available in commonly used plants. In this study, purified extracts isolated from hops—alpha and beta acids and xanthohumol—were tested in vivo for their inhibitory effect against C. difficile. A rat model of the peroral intestinal infection by C. difficile has been developed. The results show that both xanthohumol and beta acids from hops exert a notable antimicrobial effect in the C. difficile infection. The xanthohumol application showed the most pronounced antimicrobial effect together with an improvement of local inflammatory signs in the large intestine. Thus, the hops compounds represent promising antimicrobial agents for the treatment of intestinal infections caused by C. difficile.
- Published
- 2021
- Full Text
- View/download PDF
28. Visual Diagnosis of the Varroa Destructor Parasitic Mite in Honeybees Using Object Detector Techniques
- Author
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Simon Bilik, Lukas Kratochvila, Adam Ligocki, Ondrej Bostik, Tomas Zemcik, Matous Hybl, Karel Horak, and Ludek Zalud
- Subjects
Varroa destructor ,Apis mellifera ,western honey bee ,bee health monitoring ,object detection ,YOLO ,Chemical technology ,TP1-1185 - Abstract
The Varroa destructor mite is one of the most dangerous Honey Bee (Apis mellifera) parasites worldwide and the bee colonies have to be regularly monitored in order to control its spread. In this paper we present an object detector based method for health state monitoring of bee colonies. This method has the potential for online measurement and processing. In our experiment, we compare the YOLO and SSD object detectors along with the Deep SVDD anomaly detector. Based on the custom dataset with 600 ground-truth images of healthy and infected bees in various scenes, the detectors reached the highest F1 score up to 0.874 in the infected bee detection and up to 0.714 in the detection of the Varroa destructor mite itself. The results demonstrate the potential of this approach, which will be later used in the real-time computer vision based honey bee inspection system. To the best of our knowledge, this study is the first one using object detectors for the Varroa destructor mite detection on a honey bee. We expect that performance of those object detectors will enable us to inspect the health status of the honey bee colonies in real time.
- Published
- 2021
- Full Text
- View/download PDF
29. Effective Treatment for Uncomplicated Urinary Tract Infections with Oral Fosfomycin, Single Center Four Year Retrospective Study
- Author
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Miroslav Fajfr, Michal Balik, Eva Cermakova, and Pavel Bostik
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fosfomycin ,urinary infection ,resistance ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Fosfomycin represents a relatively old antibiotic, but it is experiencing a comeback in recent years. According to some studies, the increasing therapeutic use of this drug led to a rapid increase in the levels of resistance in bacteria causing urinary tract infection. In the presented study, levels of resistance to fosfomycin in more than 3500 bacterial isolates before and after fosfomycin introduction into therapeutic use in the Czech Republic and the clinical efficacy of treatment in 300 patients using this drug were assessed. The results show that the resistance levels to fosfomycin in Escherichia coli isolates before and after the drug registration were not significantly different (3.4% and 4.4%, respectively). In some other Gram-negative rods, such as otherwise susceptible Enterobacter, resistance to fosfomycin increased significantly from 45.6% to 76.6%. Fosfomycin treatment of urinary tract infections showed an excellent seven-day clinical efficacy (79.7%). However, when used to treat recurrent or complicated urinary tract infections, fosfomycin treatment was associated with high levels of infection relapse, leading to relapse in a total of 20.4% of patients during the first two months. This indicates that fosfomycin exhibits good efficacy only for the treatment of uncomplicated urinary tract infections
- Published
- 2020
- Full Text
- View/download PDF
30. Antibiotic Resistance in Nosocomial Bacteria Isolated from Infected Wounds of Hospitalized Patients in Czech Republic
- Author
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Milan Kolar, Pavel Cermak, Lenka Hobzova, Katerina Bogdanova, Katerina Neradova, Patrik Mlynarcik, and Pavel Bostik
- Subjects
nosocomial ,wound infection ,resistance ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Hospitalized patients with wounds face an increased risk of infection with multi-drug-resistant nosocomial bacteria. In this study, samples from almost 10,000 patients from big hospitals in Czech Republic with infected wounds were analyzed for the presence of bacterial pathogens. In 7693 patients (78.8%), bacterial etiological agents were identified. Members of the Enterobacterales (37.1%) and Staphyloccus aureus (21.1%) were the most prevalent pathogens. Staphyloccus aureus showed methicillin resistance in 8.6%. Almost half of the Klebsiella pneumoniae isolates were ESBL-positive and 25.6% of the Enterobacter spp. isolates were AmpC-positive. The third most prevalent Pseudomonas aeruginosa showed resistance to 19–32% of the antipseudomonal antibiotics tested. Based on the results, amoxicillin/clavulanic acid, ampicillin/sulbactam or piperacillin/tazobactam combined with gentamicin can be recommended for antibiotic treatment of infected wounds. Once the etiological agent is identified, the therapy should be adjusted according to the species and its resistance.
- Published
- 2020
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- View/download PDF
31. Usability Evaluation of Randomly Generated Fonts for Bubble Captcha
- Author
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Ondrej Bostik, Karel Horak, and Jan Klecka
- Subjects
OCR ,CAPTCHA ,Neural Networks ,k-NN ,Decision trees ,SVM ,Electronic computers. Computer science ,QA75.5-76.95 - Abstract
A Completely Automated Public Turing test to tell Computers and Humans Apart (CAPTCHA), is the wide-spread concept of systems suited to secure the web services from automated SPAM scripts. The most common CAPTCHA systems benefit from imperfections of Optical Character Recognition algorithms. This paper presents our ongoing work focused on the development of a new CAPTCHA scheme based on a human perception. The goal of this work is to evaluate the usability of randomly generated fonts used in Bubble Captcha scheme with both humans and OCR classifiers.
- Published
- 2018
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32. Bubble Captcha - A Start of the New Direction of Text Captcha Scheme Development
- Author
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Ondrej Bostik, Karel Horak, and Jan Klecka
- Subjects
Electronic computers. Computer science ,QA75.5-76.95 - Abstract
CAPTCHA, A Completely Automated Public Turing test to tell Computers and Humans Apart, is well-known system widely used in all sorts of internet services around the world designated to secure the web from an automatic malicious activity. For almost two decades almost every system utilize a simple approach to this problem containing a transcription of distorted letters from image to a text eld. The ground idea is to use imperfection of Optical Character Recognition algorithms against the computers. The development of Optical Character recognition algorithms leads only to state, where the CAPTCHA schemes become more complex and human users have a great di culty with the transcription. This paper aims to present a new way of development of CAPTCHA schemes based more a human perception. The goal of this work is to implement new Captcha scheme and assess human capability to read unusual fonts newer seen before.
- Published
- 2017
- Full Text
- View/download PDF
33. Healing from Suicide: Adolescent Perceptions of Attachment Relationships
- Author
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Bostik, Katherine E. and Everall, Robin D.
- Abstract
Relatively little is known about how adolescents overcome being suicidal. The purpose of this study was to develop an understanding of adolescents' perceptions of the role of attachment relationships in the process of overcoming suicidality. Forty-one female and nine male adolescents, previously suicidal between the ages of 13 and 19, were interviewed. Qualitative data were analysed using a grounded theory approach. The study identified three main categories: (a) attachment relationships; (b) experiences of attachment; and (c) changing self-perceptions. Participants consistently reported the creation of a secure attachment as being pivotal to healing from suicidal feelings. Implications for counselling practice are discussed. (Contains 1 table and 1 note.)
- Published
- 2007
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34. Being in the Safety Zone: Emotional Experiences of Suicidal Adolescents and Emerging Adults
- Author
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Everall, Robin D., Bostik, Katherine E., and Paulson, Barbara L.
- Abstract
This study examined how previously suicidal adolescents and emerging adults perceived their emotional experiences while being suicidal. Forty-one females and 9 males previously suicidal between the ages of 15 and 24 were interviewed. Qualitative data were analyzed using a grounded theory approach. Three major themes were identified in participants' perceptions of their emotional experiences: (a) overwhelming despair, (b) shame and self-loathing, and (c) alienation and personal isolation. These emotional states were viewed within the multidimensional process of responding to emotions. Participants' inability to deal with intense negative emotions appeared to be related to their suicidal state.
- Published
- 2006
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- View/download PDF
35. I'm Sick of Being Me: Developmental Themes in a Suicidal Adolescent
- Author
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Everall, Robin D., Bostik, Katherine E., and Paulson, Barbara L.
- Abstract
Adolescence is a developmental transition period during which there are profound transformations in emotional, cognitive, and behavioral systems. Despite being a time of rapid development and increasing rates of suicidality, limited research has examined possible interrelationships. Through the use of a case study, this paper illustrates the role of developmental factors in a teenage girl's experience becoming and overcoming being suicidal. The processes of cognitive development, identity formation, and autonomy-seeking are discussed within the context of her story.
- Published
- 2005
36. Antenna Arrays for Tactical Communication Systems: A Comparative Study
- Author
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L. Slama, J. Vorek, D. Wolansky, P. Kovacs, T. Mikulasek, J. Puskely, Z. Raida, J. Bartyzal, and T. Bostik
- Subjects
Planar antenna array ,tactical communication systems ,aperture-fed antenna ,stacked-patch antenna ,E-shaped patch antenna ,U-slot patch antenna ,feeding network ,Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 - Abstract
In this paper, we give a comparative study of several planar antenna concepts for reliable long range links in a tactical environment. The antenna elements are studied in terms of their electrical properties (bandwidth, reflection coefficient and radiation characteristics) and construction (robustness and material consumption). First, we model single antenna elements to investigate if they meet the requirements. Second, we arrange the elements with the best features into 2x2 arrays. Computer simulations of the arrays are verified by measurements. Finally, we formulate recommendations for large array (8x8 or 16x16 elements) synthesis to achieve the required properties.
- Published
- 2011
37. Reactivation of Human Acetylcholinesterase and Butyrylcholinesterase Inhibited by Leptophos-Oxon with Different Oxime Reactivators in Vitro
- Author
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Pavel Bostik, Kamil Kuca, Young-Sik Jung, Miroslav Pohanka, Lucie Musilova, and Daniel Jun
- Subjects
acetylcholinesterase ,butyrylcholinesterase ,nerve agent ,pesticide ,reactivator ,oxime ,scavenger ,leptophos-oxon ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
We have evaluated in vitro the potency of 23 oximes to reactivate human erythrocyte acetylcholinesterase (AChE) and plasma butyrylcholinesterase (BChE) inhibited by racemic leptophos-oxon (O-[4-bromo-2,5-dichlorophenyl]-O-methyl phenyl-phosphonate), a toxic metabolite of the pesticide leptophos. Compounds were assayed in concentrations of 10 and 100 µM. In case of leptophos-oxon inhibited AChE, the best reactivation potency was achieved with methoxime, trimedoxime, obidoxime and oxime K027. The most potent reactivators of inhibited BChE were K033, obidoxime, K117, bis-3-PA, K075, K074 and K127. The reactivation efficacy of tested oximes was lower in case of leptophos-oxon inhibited BChE.
- Published
- 2010
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- View/download PDF
38. The Development of Mouse APECED Models Provides New Insight into the Role of AIRE in Immune Regulation
- Author
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Aftab A. Ansari, Pavel Bostik, and Lara E. Pereira
- Subjects
Immunologic diseases. Allergy ,RC581-607 - Published
- 2005
- Full Text
- View/download PDF
39. Genetic Analysis of Cytokine Promoters in Nonhuman Primates: Implications for Th1/Th2 Profile Characteristics and SIV Disease Pathogenesis
- Author
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Pavel Bostik, Melanie Watkins, Francois Villinger, and Aftab A. Ansari
- Subjects
Immunologic diseases. Allergy ,RC581-607 - Abstract
The shift from a predominant synthesis of prototype Th1 cytokines to Th2 or Th0 type of cytokines by antigen activated PBMC's from HIV infected humans and SIV infected disease susceptible rhesus macaques (RM) has been shown to be associated with disease progression. Paradoxically, antigen activated PBMC's from sooty mangabeys (SM), which are naturally infected with SIV and are disease resistant despite high viral loads, maintain a predominant Th2 cytokine profile. It has been reasoned that the resistance to perturbations of cytokine synthesis by slow and/or nonprogressor HIV infected patients and SIV infected disease susceptible RM is secondary to inherited polymorphisms within the promoter regions for cytokines. Similar promoter polymorphisms could also contribute to the cytokine profile of PBMC's from SM. To address this issue promoter regions for the major Th1/Th2 cytokines from RM and SM were cloned and sequenced. Sequence analysis of promoter fragments of IL-4, IL-10, IL-12 p40, IFN-gamma and TNF-alpha from the two monkey species showed varying degree of homology ranging from high degree of homology detected for IFN-gamma promoter (>99%) to relatively high degree of polymorphism detected for TNF-alpha promoter (94% homology). In addition, several variable regions within the promoters of IL-12 p40, IL-10 and TNF-alpha in the two species contain polymorphisms in sequences that constitute binding sites of known transcription factors (TF). Such differences are likely to differentially bind TF and thus either qualitatively and/or quantitatively affect the regulation of cytokine synthesis in these two species and potentially contribute to disease progression and/or resistance.
- Published
- 2004
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- View/download PDF
40. Sin Nombre Virus (SNV) Ig Isotype Antibody Response during Acute and Convalescent Phases of Hantavirus Pulmonary Syndrome
- Author
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Pavel Bostik, Jorn Winter, Thomas G. Ksiazek, Pierre E. Rollin, Francois Villinger, Sherif R. Zaki, C.J. Peters, and Aftab A. Ansari
- Subjects
hantavirus pulmonary syndrome ,hantaviruses ,HPS ,Sin Nombre virus ,SNV ,United States ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Abstract
Serum samples from 22 hantavirus pulmonary syndrome (HPS) patients were tested for Sin Nombre virus (SNV)-reactive antibodies. In the acute phase of HPS, 100% and 67% of the samples tested positive for SNV-specific immunoglobulin (Ig) M and IgA, respectively. Among the virus-specific IgG antibodies, the most prevalent were IgG3 (in 97% of samples), followed by IgG1 (70%), IgG2 (30%), and IgG4 (3%).
- Published
- 2000
- Full Text
- View/download PDF
41. Simple Method for Selective Amplification of cDNA from a Defined Promoter
- Author
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Pavel Bostik, Lubomir P. Turek, and Thomas H. Haugen
- Subjects
Biology (General) ,QH301-705.5 - Abstract
A simplified technique for the detection of transcripts from a defined promoter is described. After reverse transcription, a PCR target sequence is selectively added to the 3′ end of cDNA strands by DNA polymerase extension directed by an oligonucleotide template. Those cDNA molecules that do not have ends within a few nucleotides of the promoter start site are not extended and thus are excluded from subsequent amplification. Even when amplified products are visualized by ethidium bromide staining of agarose gels, this method requires only 1% of the RNA usually needed for detection of mRNA by standard RNase protection utilizing radiolabeled probes. In contrast to direct detection of cDNA by PCR, this procedure restricts amplification to a narrow subset of transcripts even when other overlapping colinear transcripts are present. We call this detection procedure specific amplification of cDNA ends (SPACE).
- Published
- 1997
- Full Text
- View/download PDF
42. In My Mind I Was Alone: Suicidal Adolescents' Perceptions of Attachment Relationships
- Author
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Bostik, Katherine E. and Everall, Robin D.
- Published
- 2006
- Full Text
- View/download PDF
43. Cloning, sequencing, and homology analysis of nonhuman primate Fas/Fas-ligand and co-stimulatory molecules
- Author
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Villinger, Francois, Bostik, Pavel, Mayne, Ann, King, Christopher L., Genain, Claude P., Weiss, Walter R., and Ansari, Aftab A.
- Published
- 2001
- Full Text
- View/download PDF
44. IL-15 is superior to IL-2 in the generation of long-lived antigen specific memory CD4 and CD8 T cells in rhesus macaques
- Author
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Villinger, F., Miller, R., Mori, K., Mayne, A.E., Bostik, P., Sundstrom, J.B., Sugimoto, C., and Ansari, A.A.
- Published
- 2004
- Full Text
- View/download PDF
45. Distinct host cell proteins incorporated by SIV replicating in CD4+ T Cells from natural disease resistant versus non-natural disease susceptible hosts
- Author
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Rehulka Pavel, Bhimani Samrath, Rajan Devi, Song Byeongwoon, Bostik Pavel, Stephenson Susan T, Mayne Ann E, and Ansari Aftab A
- Subjects
Immunologic diseases. Allergy ,RC581-607 - Abstract
Abstract Background Enveloped viruses including the simian immunodeficiency virus (SIV) replicating within host cells acquire host proteins upon egress from the host cells. A number of studies have catalogued such host proteins, and a few have documented the potential positive and negative biological functions of such host proteins. The studies conducted herein utilized proteomic analysis to identify differences in the spectrum of host proteins acquired by a single source of SIV replicating within CD4+ T cells from disease resistant sooty mangabeys and disease susceptible rhesus macaques. Results While a total of 202 host derived proteins were present in viral preparations from CD4+ T cells from both species, there were 4 host-derived proteins that consistently and uniquely associated with SIV replicating within CD4+ T cells from rhesus macaques but not sooty mangabeys; and, similarly, 28 host-derived proteins that uniquely associated with SIV replicating within CD4+ T cells from sooty mangabeys, but not rhesus macaques. Of interest was the finding that of the 4 proteins uniquely present in SIV preparations from rhesus macaques was a 26 S protease subunit 7 (MSS1) that was shown to enhance HIV-1 'tat" mediated transactivation. Among the 28 proteins found in SIV preparations from sooty mangabeys included several molecules associated with immune function such as CD2, CD3ε, TLR4, TLR9 and TNFR and a bioactive form of IL-13. Conclusions The finding of 4 host proteins that are uniquely associated with SIV replicating within CD4+ T cells from disease susceptible rhesus macaques and 28 host proteins that are uniquely associated with SIV replicating within CD4+ T cells from disease resistant sooty mangabeys provide the foundation for determining the potential role of each of these unique host-derived proteins in contributing to the polarized clinical outcome in these 2 species of nonhuman primates.
- Published
- 2010
- Full Text
- View/download PDF
46. Inhibitory effect of hop fractions against Gram-positive multi-resistant bacteria. A pilot study.
- Author
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Bogdanova, Katerina, Kolar, Milan, Langova, Katerina, Dusek, Martin, Mikyska, Alexandr, Bostikova, Vanda, Bostik, Pavel, and Olsovska, Jana
- Abstract
Aim. Our research focused on the antimicrobial effects of purified hop (Humulus lupulus L.) fractions including α-bitter acids (humulones), β-bitter acids (lupulones) and xanthohumol, and a commercial CO2 hop extract of bitter acids against reference and multi-resistant strains of Gram-positive and Gram-negative bacteria and against selected yeast strains. Methods. In vitro testing of antimicrobial activity was performed according to standard testing protocols (EUCAST). The effects of hop extracts on bacterial/yeast strains at concentrations below MICs were also determined and the antimicrobial potential of hop extracts was compared with selected antibiotics using optical density measurement. Results. The fractions were effective not only against reference strains of Gram-positive bacteria but, more importantly, against their methicillin- and vancomycin-resistant variants. No antimicrobial effect was detected against Gram-negative bacterial strains. Among the tested substances, xanthohumol was identified as the hop fraction with the most potent antimicrobial properties. It was also found that hop substances exerted their antimicrobial effects at concentrations considerably lower than the determined MICs, with the strongest effect in case of α-bitter acids in enterococci. Conclusion. The search for and research of new compounds with antimicrobial properties represents a possible solution to the current global problem of bacterial resistance. Our data suggest a desirable activity of hop fractions against some multi-resistant bacterial strains. Thus, hops might find use as a source of potential antimicrobial agents applicable in both human and veterinary medicine. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
47. A repeated syphilis infection imported from Thailand in an HIV positive couple of men-who-have-sex-with-men in Czech Republic.
- Author
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Salavec, Miloslav, Bostik, Vanda, Kapla, Jaroslav, Plisek, Stanislav, Prasil, Petr, Prymula, Roman, and Bostik, Pavel
- Abstract
Coinfection by HIV and syphilis has become a growing problem due to the reappearance of unsafe sexual practices in the era of potent anti-retroviral drugs. We describe a repeated import of syphilis by a couple of men-who-have-sex-with-men from Thailand to Czech Republic likely due to non-adherence of the patients to physician recommendations. Such cases can become foci for dissemination of once locally rare infections and present a danger for the community. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
48. Recognition of CAPTCHA Characters by Supervised Machine Learning Algorithms
- Author
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Bostik, Ondrej and Klecka, Jan
- Abstract
The focus of this paper is to compare several common machine learning classification algorithms for Optical Character Recognition of CAPTCHA codes. The main part of a research focuses on the comparative study of Neural Networks, k-Nearest Neighbour, Support Vector Machines and Decision Trees implemented in MATLAB Computing environment. Achieved success rates of all analyzed algorithms overcome 89%. The main difference in results of used algorithms is within the learning times. Based on the data found, it is possible to choose the right algorithm for the particular task.
- Published
- 2018
- Full Text
- View/download PDF
49. Ascorbic Acid: An Old Player with a Broad Impact on Body Physiology Including Oxidative Stress Suppression and Immunomodulation: A Review
- Author
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Pohanka, M., primary, Pejchal, J., additional, Snopkova, S., additional, Havlickova, K., additional, Z. Karasova, J., additional, Bostik, P., additional, and Pikula, J., additional
- Published
- 2012
- Full Text
- View/download PDF
50. Macrophage-Assisted Inflammation and Pharmacological Regulation of the Cholinergic Anti-Inflammatory Pathway
- Author
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Pohanka, M., primary, Snopkova, S., additional, Havlickova, K., additional, Bostik, P., additional, Sinkorova, Z., additional, Fusek, J., additional, Kuca, K., additional, and Pikula, Jiri, additional
- Published
- 2011
- Full Text
- View/download PDF
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