45 results on '"Bosisio, C"'
Search Results
2. P-745 Telemedicine during the COVID -19 pandemic: let us give the floor to the patients
- Author
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Arnoldi, M, primary, Candeloro, I, additional, Lombardo, G, additional, Cappellari, M, additional, Bosisio, C, additional, Galbignani, E, additional, Cappato, M, additional, and Fusi, F.M, additional
- Published
- 2022
- Full Text
- View/download PDF
3. Long-Term Mental and Physical Quality of Life after Radical and Conservative Surgeries and in Relation to Childbirth in Patients with Severe Endometriosis
- Author
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Betto, E, Zarbo, C, Verrengia, A, Malandrino, C, Secomandi, R, Trezzi, G, Bosisio, C, Rabboni, M, Compare, A, Frigerio, L, Betto, Enrico, Zarbo, Cristina, Verrengia, Apollonia., Malandrino, Chiara, Secomandi, Rita, Trezzi, Gaetano, Bosisio, Chiara, Rabboni, Massimo, Compare, Angelo, Frigerio, Luigi, Betto, E, Zarbo, C, Verrengia, A, Malandrino, C, Secomandi, R, Trezzi, G, Bosisio, C, Rabboni, M, Compare, A, Frigerio, L, Betto, Enrico, Zarbo, Cristina, Verrengia, Apollonia., Malandrino, Chiara, Secomandi, Rita, Trezzi, Gaetano, Bosisio, Chiara, Rabboni, Massimo, Compare, Angelo, and Frigerio, Luigi
- Abstract
Aim: This study aimed to investigate which surgery (conservative, radical) is the most effective in improving quality of life and if childbirth after conservative one affects outcomes. Method: 79 women who underwent radical or conservative surgery for severe endometriosis were assessed by means of a semistructured interview and completed the SF-36. Results: Our findings showed that in the whole model of Hierarchical multiple linear regression analyses, surgery group explained significantly 10.7% of variance for bodily pain, 7.2% for general health, and 7.9% for vitality, independently of age and months spent since surgery. Groups of childbirth differed significantly for vitality (p .018, Cohen’s d .939) and role emotional (p .034, Cohen’s d .786). Discussion: Findings suggested that women with endometriosis who underwent radical surgery showed better quality of life in long term, independently of age and time spent since treatment. Moreover, women who gave birth to a child had a better mental quality of life, especially concerning vitality and role limitations due to emotional problems.
- Published
- 2017
4. Protonation and conformational dynamics of GFP mutants by two-photons excitation fluorescence correlation spectroscopy
- Author
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Bosisio, C., Quercioli, V., Collini, M., D'Alfonso, L., Baldini, G., Bettani, S., Campanini, B., Raboni, S., and Chirico, G.
- Subjects
Fluorescence -- Analysis ,Gene mutations -- Analysis ,Chemicals, plastics and rubber industries - Abstract
The role of histidine 148 and glutamate 222 is analyzed by studying the fluorescence dynamics of green fluorescence protein mutant (GFPmut) and its H148G and E222Q mutants. The power dependent but pH-independent relaxation mode is due to an excited-state process that is related to conformational rearrangements of the chromophore after the photoexcitation, more than to the chromophore excited-state proton transfer.
- Published
- 2008
5. Retorica del notiziario nell'Italia contemporanea
- Author
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Bosisio, C, Cambiaghi, B, Piemontese, E, Santulli, F, Bosisio, C ( C ), Cambiaghi, B ( B ), Piemontese, E ( E ), Santulli, F ( F ), Loporcaro, M, Bosisio, C, Cambiaghi, B, Piemontese, E, Santulli, F, Bosisio, C ( C ), Cambiaghi, B ( B ), Piemontese, E ( E ), Santulli, F ( F ), and Loporcaro, M
- Published
- 2008
6. Dictionnaire de didactique du français Langue étrangère et seconde
- Author
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Galazzi E, Bosisio C, Molinari C, SPIEZIA, Raffaele, Galazzi, E, Bosisio, C, Molinari, C, and Spiezia, Raffaele
- Subjects
Langue seconde ,Didactique du françai ,Langue étrangère - Abstract
209-033972-1
- Published
- 2003
7. introduzione
- Author
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Cavagnoli, Stefania and Bosisio, C.
- Published
- 2013
8. Precision electroweak measurements on the Z resonance
- Author
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Schael, S, Barate, R, Bruneliere, R, Buskulic, D, Bonis, De, Decamp, I, Ghez, D, Goy, P, Jezequel, C, Lees, S, Lucotte, Jp, Martin, A, Merle, F, Minard, E, Nief, Mn, Odier, Jy, Pietrzyk, P, Trocme, B, Bravo, B, Casado, S, Chmeissani, Mp, Comas, M, Crespo, P, Fernandez, Jm, E, Fernandez, Bosman, Garrido, M, Grauges, L, Juste, E, Martinez, A, Merino, M, Miquel, G, Mir, R, Orteu, Lm, Pacheco, S, Park, A, Perlas, Ic, Riu, J, Ruiz, I, Sanchez, H, Colaleo, F, Creanza, A, D, Filippis, De, N, Palma, De, Iaselli, M, Maggi, G, Nuzzo, M, Ranieri, S, Raso, A, Ruggieri, G, Selvaggi, F, Silvestris, G, Tempesta, L, Tricomi, P, Zito, A, Huang, G, Lin, X, Ouyang, J, Wang, Q, Xie, T, Xu, Y, Xue, R, Zhang, S, Zhang, J, Zhao, L, Abbaneo, W, Bazarko, D, Becker, A, Boix, U, Bird, G, Blucher, F, Bonvicini, E, B, Bright, Thomas, Barklow, P, Buchmuller, T, Cattaneo, O, Cerutti, M, Ciulli, F, Clerbaux, V, Drevermann, B, Forty, H, Frank, Rw, Greening, M, Hagelberg, Tc, Halley, R, Gianotti, Aw, Girone, F, Hansen, M, Harvey, Jb, Jacobsen, J, Hutchcroft, R, Janot, De, Jost, R, Knobloch, B, Kado, J, Lehraus, M, Lazeyras, I, Maley, P, Mato, R, May, P, Moutussi, J, A, Pepe, Altarelli, Ranjard, M, Rolandi, F, Schlatter, L, Schmitt, D, Schneider, B, Tejessy, O, Teubert, W, Tomalin, F, Tournefier, Ir, Veenhof, E, Valassi, R, Wiedenmann, A, Wright, W, Ajaltouni, Ae, Badaud, Z, Chazelle, F, Deschamps, G, Dessagne, O, Falvard, S, Ferdi, A, Fayolle, C, Gay, D, Guicheney, P, Henrard, C, Jousset, P, Michel, J, Monteil, B, Montret, S, Pallin, Jc, Pascolo, D, Perret, Jm, Podlyski, P, Bertelsen, F, Fernley, H, Hansen, T, Hansen, Jd, Hansen, Jr, Kraan, Ph, Lindahl, Ac, Mollerud, A, Nilsson, R, Rensch, Bs, Waananen, B, Daskalakis, A, Kyriakis, G, Markou, A, Simopoulou, C, Siotis, E, Vayaki, I, Zachariadou, A, Blondel, K, Bonneaud, A, Brient, G, Machefert, Jc, Rouge, E, Rumpf, A, Swynghedauw, M, Tanaka, M, Verderi, R, Videau, M, Focardi, H, Parrini, E, Corden, G, Georgiopoulos, M, Antonelli, C, Antonelli, A, Bencivenni, M, Bologna, G, Bossi, G, Campana, F, Capon, P, Chiarella, G, Felici, V, Laurelli, G, Mannocchi, P, Murtas, G, Passalacqua, Gp, Picchi, L, Colrain, P, P, Ten, Have, Hughes, I, Kennedy, Is, Knowles, J, Lynch, Ig, Morton, Jg, Negus, Wt, Oshea, P., Raine, V, Reeves, C, Scarr, P, Smith, Jm, Thompson, K., Turnbull, As, Wasserbaech, Rm, Cavanaugh, S, Dhamotharan, R, Geweniger, S, Hanke, C, Hansper, P, Hepp, G, Kluge, V, Putzer, Ee, Sommer, A, Stenzel, J, Tittel, H, Werner, K, Wunsch, W, Beuselinck, M, Binnie, R, Cameron, Dm, Davies, W, Dornan, G, Goodsir, Pj, Marinelli, S, Martin, N, Nash, Eb, Nowell, J, Rutherford, J, Sedgbeer, Sa, Thompson, Jk, White, Jc, Williams, R, Ghete, Md, Girtler, Vm, Kneringer, P, Kuhn, E, Rudolph, D, G, Bouhova, Thacker, Bowdery, E, Buck, Ck, Clarke, Pg, Ellis, Dp, Finch, G, Foster, Aj, Hughes, F, Jones, G, Rwl, Keemer, Pearson, Nr, Robertson, Mr, Sloan, Na, Smizanska, T, Snow, M, Williams, Sw, van der Aa, Delaere, O, Leibenguth, C, Lemaitre, G, Bauerdick, V, Lat, Blumenschein, U, Van, Gemmeren, Giehl, P, Holldorfer, I, Jakobs, F, Kasemann, K, Kayser, M, Kleinknecht, F, Muller, K, Quast, As, Renk, G, Rohne, B, Sander, E, Schmeling, Hg, Wachsmuth, S, Wanke, H, Zeitnitz, R, Ziegler, C, Aubert, T, Benchouk, Jj, Bonissent, C, Carr, A, Coyle, J, Curtil, P, Ealet, C, Etienne, A, Fouchez, F, Motsch, D, Payre, F, Rousseau, P, Tilquin, D, Talby, A, Thulasides, M, Aleppo, M, Ragusa, M, Buscher, F, David, V, Dietl, A, Ganis, H, Huttmann, G, Lutjens, K, Mannert, G, Manner, C, Moser, W, Settles, Hg, Seywerd, R, Villegas, H, Wolf, M, Azzurri, G, Boucrot, P, Callot, J, Chen, O, Cordier, S, Davier, A, Duflot, M, Grivaz, L, Heusse, Jf, Jacholkowska, P, Diberder, Le, Lefrancois, F, Mutz, J, Schune, Am, Serin, Mh, Veillet, L, Videau, Jj, Zerwas, I, Bagliesi, D, Bettarini, G, Boccali, S, Bozzi, T, Calderini, C, Dellorso, G, Fantechi, R, Ferrante, R, Fidecaro, I, Foa, F, Giammanco, L, Giassi, A, Gregorio, A, Ligabue, A, Lusiani, F, Marrocchesi, PIER SIMONE, Messineo, Ps, Palla, A, Rizzo, F, Sanguinetti, G, Sciaba, G, Sguazzoni, A, Spagnolo, G, Steinberger, P, Tenchini, J, Venturi, R, Vannini, A, Verdini, C, Awunor, Pg, Blair, O, Cowan, Ga, Garcia, Bellido, Green, A, Medcalf, Mg, Misiejuk, T, Strong, A, Teixeira, Dias, Botterill, P, Clifft, Dr, Edgecock, Rw, Edwards, Tr, Haywood, M, Norton, Sj, Ward, Pr, Bloch, Devaux, Boumediene, B, Colas, D, Emery, P, Fabbro, S, Kozanecki, B, Lancon, W, Lemaire, E, Locci, Mc, Perez, E, Rander, P, Renardy, J, Roussarie, Jf, Schuller, A, Schwindling, Jp, Tuchming, J, Vallage, B, Black, B, Dann, Sn, Kim, Jh, Konstantinidis, Hy, Litke, N, Mcneil, Am, Taylor, Ma, Booth, G, Cartwright, Cn, Combley, S, Hodgson, F, Lehto, Pn, Thompson, M, Affholderbach, Lf, Barberio, K, Bohrer, E, Brandt, A, Burkhardt, S, Feigl, H, Grupen, E, Hess, C, Lutters, J, Meinhard, G, H, Minguet, Rodriguez, Mirabito, J, Neugebauer, L, Ngac, E, Prange, A, Rivera, G, Saraiva, F, Schafer, P, Sieler, U, Smolik, U, Stephan, L, Trier, F, Apollonio, H, Borean, M, Bosisio, C, L, Della, Marina, Giannini, R, Gobbo, G, Musolino, B, Pitis, G, He, L, Kim, H, Putz, H, Rothberg, J, Armstrong, J, Bellantoni, Sr, Berkelman, L, Cinabro, K, Conway, D, Cranmer, Js, Elmer, K, Feng, P, Ferguson, Z, Dps, Gao, Gonzalez, Y, Grahl, S, Harton, J, Hayes, Jl, Hu, Oj, Jin, H, Johnson, S, Kile, Rp, Mcnamara, J, Nielsen, Pa, Orejudos, J, Pan, W, Saadi, Y, Scott, Y, Sharma, Ij, Walsh, V, Walsh, Am, Wear, J, J, von Wimmersperg Toeller, Wu, Jh, Wu, J, Wu, Sl, Yamartino, X, Zobernig, Jm, Dissertori, G, Abdallah, G, Abreu, J, Adam, P, Adye, W, Adzic, T, Ajinenko, P, Albrecht, I, Alderweireld, T, Alekseev, T, Alemany, Fernandez, Allmendinger, R, Allport, T, Almehed, Pp, Amaldi, S, Amapane, U, Amato, N, Anashkin, S, Anassontzis, E, Andersson, Eg, Andreazza, P, Andringa, A, Anjos, S, Antilous, N, Apel, P, Arnoud, Wd, Ask, Y, Asman, S, Augustin, B, Augustinus, Je, Baillon, A, Ballestrero, P, Bambade, A, Barao, P, Barbiellini, F, Barbier, G, Bardin, R, Barker, D, Baroncelli, G, Battaglia, A, Baubillier, M, Becks, M, Begalli, Kh, Behrmann, M, Beilliere, A, Belokopytov, P, Belous, Y, K, Ben, Haim, Benekos, E, Benvenuti, N, Berat, A, Berggren, C, Berntzon, M, Bertini, L, Bertrand, D, Besancon, D, Besson, M, Bianchi, N, Bigi, F, Bilenky, M, Bizouard, Ms, Bloch, Ma, Blom, D, Bluj, M, Bonesini, M, Bonivento, M, Boonekamp, W, Booth, M, Psl, Borgland, Borisov, Aw, Bosio, G, Botner, C, Boudinov, O, Bouquet, E, Bourdarios, B, Bowcock, C, Tjv, Boyko, Bozovic, I, Bozzo, I, Bracko, M, Branchini, M, Brenke, P, Brenner, T, Brodet, R, Bruckman, E, Brunet, P, Bugge, Jm, Buran, L, Burgsmueller, T, Buschbeck, T, Buschmann, B, Cabrera, P, Caccia, S, Calvi, M, Rozas, M, Ajc, Camporesi, Canale, T, Canepa, V, Carena, M, Carroll, F, Caso, L, Gimenez, C, Mvc, Castro, Cattai, N, Cavallo, A, Cerruti, F, Chabaud, C, Chapkin, V, Charpentier, M, Chaussard, P, Checchia, L, Chelkov, P, Chen, Ga, Chierici, M, Chliapnikov, R, Chochula, R, Chorowicz, P, Chudoba, V, Chung, J, Cieslik, Su, Collins, K, Colomer, P, Contri, M, Cortina, R, Cosme, E, Cossuti, G, Costa, F, Cowell, Mj, Crawley, Jh, Crennell, Hb, Crepe, D, Crosetti, S, Cuevas, G, Czellar, J, Dhondt, S, Dalmagne, J, Dalmau, B, Damgaard, J, Davenport, G, M, Silva, Da, T, Deghorain, W, Della, Ricca, Delpierre, G, Demaria, P, Angelis, De, Boer, De, W, Brabandere, De, S, Clercq, De, C, Lotto, De, Maria, De, Min, De, Paula, De, Dijkstra, L, Ciaccio, Di, Diodato, Di, Simone, Di, Djannati, A, Dolbeau, A, Doroba, J, Dracos, K, Drees, M, Drees, J, Dris, Ka, Duperrin, M, Durand, A, Ehret, Jd, Eigen, R, Ekelof, G, Ekspong, T, Ellert, G, Elsing, M, Engel, M, Erzen, Jp, Santo, B, Mce, Falk, Fanourakis, E, Fassouliotis, G, Fayot, D, Feindt, J, Fenyuk, M, Fernandez, A, Ferrari, J, Ferrer, P, Ferrer, Ribas, Ferro, E, Fichet, F, Firestone, S, Fischer, A, Flagmeyer, Pa, Foeth, U, Fokitis, H, Fontanelli, E, Franek, F, Frodesen, B, Fruhwirth, Ag, R, Fulda, Quenzer, Fuster, F, Galloni, J, Gamba, A, Gamblin, D, Gandelman, S, Garcia, M, Garcia, C, Gaspar, J, Gaspar, C, Gasparini, M, Gavillet, U, Gazis, P, Gele, E, Gerber, D, Gerdyukov, Jp, Ghodbane, L, Gil, N, Glege, I, Gokieli, F, Golob, R, Gomez, Ceballos, Goncalves, G, Caballero, P, Gopal, Ig, Gorn, G, Gorski, L, Gouz, M, Gracco, Y, Graziani, V, Green, E, Grefrath, C, Grimm, A, Gris, Hj, Grosdidier, P, Grzelak, G, Gunther, K, Guy, M, Haag, J, Hahn, C, Hahn, F, Hallgren, S, Hamacher, A, Hamilton, K, Hansen, K, Harris, J, Haug, Fj, Hauler, S, Hedberg, F, Heising, V, Hennecke, S, Henriques, M, Hernandez, R, Herquet, Jj, Herr, P, Hessing, H, Heuser, Tl, Higon, Jm, Hoffman, E, Holmgren, J, Holt, So, Holthuizen, Pj, Hoorelbeke, D, Houlden, S, Hrubec, Ma, Huber, J, Huet, M, Hughes, K, Hultqvist, Gj, Jackson, K, Jacobsson, Jn, Jalocha, R, Janik, P, Jarlskog, R, Jarlskog, C, Jarry, G, Jean, Marie, Jeans, B, Johansson, D, Johansson, Ek, Jonsson, Pd, Joram, P, Juillot, C, Jungermann, P, Kapusta, L, Karafasoulis, F, Katsanevas, K, Katsoufis, S, Keranen, E, Kernel, R, Kersevan, G, Kerzel, Bp, Khomenko, U, Khovanski, Ba, Kiiskinen, Nn, King, A, Kinvig, Bt, Kjaer, A, Klapp, Nj, Klein, O, Kluit, H, Knoblauch, P, Kokkinias, D, Konopliannikov, P, Koratzinos, A, Kostioukhine, M, Kourkoumelis, V, Kouznetsov, C, Krammer, O, Kreuter, M, Kriznic, C, Krstic, E, Krumstein, J, Kubinec, Z, Kucewicz, P, Kucharczyk, W, Kurowska, M, Kurvinen, J, Lamsa, K, Lanceri, J, Lane, L, Langefeld, Dw, Lapin, P, Laugier, V, Lauhakangas, Jp, Leder, R, Ledroit, G, Lefebure, F, Leinonen, V, Leisos, L, Leitner, A, Lemonne, R, Lenzen, J, Lepeltier, G, Lesiak, V, Lethuillier, T, Libby, M, Liebig, J, Liko, W, Lipniacka, D, Lippi, A, Loerstad, I, Lokajicek, B, Loken, M, Lopes, Jg, Lopez, Jh, Lopez, Femandez, Loukas, R, Lutz, D, Lyons, P, Macnaughton, L, Mahon, J, Maio, Jr, Malek, A, Malmgren, A, Tgm, Maltezos, Malychev, S, Mandl, V, Marco, F, Marco, J, Marechal, R, Margoni, B, Marin, M, Mariotti, Jc, Markou, C, Martinez, Rivero, Martinez, Vidal, 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Thaler, Fe, Thom, Jj, Trandafir, J, Turk, Ai, Usher, Jd, Vavra, T, Vella, J, Venuti, E, Verdier, Jp, Wagner, R, Waite, Sr, Walston, Ap, Wang, S, Watts, J, Weidemann, Sj, Weiss, Aw, Whitaker, Er, White, Js, Wickens, Sl, Williams, Fj, Williams, Da, Williams, Dc, Willocq, Sh, Wilson, S, Wisniewski, Rj, Wittlin, Wj, Woods, Jl, Word, M, Wright, Gb, Wyss, Tr, Yamamoto, J, Yang, Rk, Yashima, Xq, Yellin, J, Young, Sj, Yuta, Cc, Zapalac, H, Zdarko, G, Zeitlin, Rw, Zhou, C, Blondel, Alain, Schael, S, Barate, R, Bruneliere, R, Spagnolo, Stefania Antonia, S., Schael, Aloisio, Alberto, Alviggi, Mariagrazia, Canale, Vincenzo, Chiefari, Giovanni, DELLA VOLPE, Domenico, Merola, Leonardo, Napolitano, Marco, Patricelli, Sergio, Sciacca, Crisostomo, Lista, Luca, Laboratoire d'Annecy de Physique des Particules (LAPP), Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physique Corpusculaire - Clermont-Ferrand (LPC), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physique Théorique et Astroparticules (LPTA), Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Leprince-Ringuet (LLR), Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-École polytechnique (X)-Centre National de la Recherche Scientifique (CNRS), Centre de Physique des Particules de Marseille (CPPM), Aix Marseille Université (AMU)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de l'Accélérateur Linéaire (LAL), Université Paris-Sud - Paris 11 (UP11)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physique Nucléaire et de Hautes Énergies (LPNHE), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physique Subatomique et de Cosmologie (LPSC), Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Institut Polytechnique de Grenoble - Grenoble Institute of Technology-Centre National de la Recherche Scientifique (CNRS), Institut de Physique Nucléaire de Lyon (IPNL), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherches Subatomiques (IReS), Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Cancéropôle du Grand Est-Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS), Physique Corpusculaire et Cosmologie - Collège de France (PCC), Collège de France (CdF (institution))-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), ALEPH, DELPHI, L3, OPAL, SLD, BARBIELLINI AMIDEI, Guido, Bosisio, Luciano, DELLA RICCA, Giuseppe, Giannini, Gianrossano, Gregorio, Anna, Lanceri, Livio, Poropat, Paolo, Vitale, Lorenzo, Buskulic, D, De Bonis, I, Decamp, D, Ghez, P, Goy, C, Jezequel, S, Lees, J, Lucotte, A, Martin, F, Merle, E, Minard, M, Nief, J, Odier, P, Pietrzyk, B, Trocme, B, Bravo, S, Casado, M, Chmeissani, M, Comas, P, Crespo, J, Fernandez, E, Fernandez Bosman, M, Garrido, L, Grauges, E, Juste, A, Martinez, M, Merino, G, Miquel, R, Mir, L, Orteu, S, Pacheco, A, Park, I, Perlas, J, Riu, I, Ruiz, H, Sanchez, F, Colaleo, A, Creanza, D, De Filippis, N, De Palma, M, Iaselli, G, Maggi, G, Maggi, M, Nuzzo, S, Ranieri, A, Raso, G, Ruggieri, F, Selvaggi, G, Silvestris, L, Tempesta, P, Tricomi, A, Zito, G, Huang, X, Lin, J, Ouyang, Q, Wang, T, Xie, Y, Xu, R, Xue, S, Zhang, J, Zhang, L, Zhao, W, Abbaneo, D, Bazarko, A, Becker, U, Boix, G, Bird, F, Blucher, E, Bonvicini, B, Bright Thomas, P, Barklow, T, Buchmuller, O, Cattaneo, M, Cerutti, F, Ciulli, V, Clerbaux, B, Drevermann, H, Forty, R, Frank, M, Greening, T, Hagelberg, R, Halley, A, Gianotti, F, Girone, M, Hansen, J, Harvey, J, Jacobsen, R, Hutchcroft, D, Janot, R, Jost, B, Knobloch, J, Kado, M, Lehraus, I, Lazeyras, P, Maley, R, Mato, P, May, J, Moutussi, A, Pepe Altarelli, M, Ranjard, F, Rolandi, L, Schlatter, D, Schmitt, B, Schneider, O, Tejessy, W, Teubert, F, Tomalin, I, Tournefier, E, Veenhof, R, Valassi, A, Wiedenmann, W, Wright, A, Ajaltouni, Z, Badaud, F, Chazelle, G, Deschamps, O, Dessagne, S, Falvard, A, Ferdi, C, Fayolle, D, Gay, P, Guicheney, C, Henrard, P, Jousset, J, Michel, B, Monteil, S, Montret, J, Pallin, D, Pascolo, J, Perret, P, Podlyski, F, Bertelsen, H, Fernley, T, Hansen, P, Kraan, A, Lindahl, A, Mollerud, R, Nilsson, B, Rensch, B, Waananen, A, Daskalakis, G, Kyriakis, A, Markou, C, Simopoulou, E, Siotis, I, Vayaki, A, Zachariadou, K, Blondel, A, Bonneaud, G, Brient, J, Machefert, E, Rouge, A, Rumpf, M, Swynghedauw, M, Tanaka, R, Verderi, M, Videau, H, Focardi, E, Parrini, G, Corden, M, Georgiopoulos, C, Antonelli, A, Antonelli, M, Bencivenni, G, Bologna, G, Bossi, F, Campana, P, Capon, G, Chiarella, V, Felici, G, Laurelli, P, Mannocchi, G, Murtas, G, Passalacqua, L, Picchi, P, Colrain, P, Ten Have, I, Hughes, I, Kennedy, J, Knowles, I, Lynch, J, Morton, W, Negus, P, O'Shea, V, Raine, C, Reeves, P, Scarr, J, Smith, K, Thompson, A, Turnbull, R, Wasserbaech, S, Cavanaugh, R, Dhamotharan, S, Geweniger, C, Hanke, P, Hansper, G, Hepp, V, Kluge, E, Putzer, A, Sommer, J, Stenzel, H, Tittel, K, Werner, W, Wunsch, M, Beuselinck, R, Binnie, D, Cameron, W, Davies, G, Dornan, P, Goodsir, S, Marinelli, N, Martin, E, Nash, J, Nowell, J, Rutherford, S, Sedgbeer, J, Thompson, J, White, R, Williams, M, Ghete, V, Girtler, P, Kneringer, E, Kuhn, D, Rudolph, G, Bouhova Thacker, E, Bowdery, C, Buck, P, Clarke, D, Ellis, G, Finch, A, Foster, F, Hughes, G, Jones, R, Keemer, N, Pearson, M, Robertson, N, Sloan, T, Smizanska, M, Snow, S, Van Der Aa, O, Delaere, C, Leibenguth, G, Lemaitre, V, Bauerdick, L, Blumenschein, U, Van Gemmeren, P, Giehl, I, Holldorfer, F, Jakobs, K, Kasemann, M, Kayser, F, Kleinknecht, K, Muller, A, Quast, G, Renk, B, Rohne, E, Sander, H, Schmeling, S, Wachsmuth, H, Wanke, R, Zeitnitz, C, Ziegler, T, Aubert, J, Benchouk, C, Bonissent, A, Carr, J, 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Edgecock, T, Edwards, M, Haywood, S, Norton, P, Ward, J, Bloch Devaux, B, Boumediene, D, Colas, P, Emery, S, Fabbro, B, Kozanecki, W, Lancon, E, Lemaire, M, Locci, E, Perez, P, Rander, J, Renardy, J, Roussarie, A, Schuller, J, Schwindling, J, Tuchming, B, Vallage, B, Black, S, Dann, J, Kim, H, Konstantinidis, N, Litke, A, Mcneil, M, Taylor, G, Booth, C, Cartwright, S, Combley, F, Hodgson, P, Lehto, M, Thompson, L, Affholderbach, K, Barberio, E, Bohrer, A, Brandt, S, Burkhardt, H, Feigl, E, Grupen, C, Hess, J, Lutters, G, Meinhard, H, Minguet Rodriguez, J, Mirabito, L, Neugebauer, E, Ngac, A, Prange, G, Rivera, F, Saraiva, P, Schafer, U, Sieler, U, Smolik, L, Stephan, F, Trier, H, Apollonio, M, Borean, C, Bosisio, L, Della Marina, R, Giannini, G, Gobbo, B, Musolino, G, Pitis, L, He, H, Putz, J, Rothberg, J, Armstrong, S, Bellantoni, L, Berkelman, K, Cinabro, D, Conway, J, Cranmer, K, Elmer, P, Feng, Z, Ferguson, D, Gao, Y, Gonzalez, S, Grahl, J, Harton, J, Hayes, O, Hu, H, Jin, S, 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MCGOWAN, A.K. MCKEMEY, B.T. MEADOWS, R. MESSNER, P.M. MOCKETT, K.C. MOFFEIT, T.B. MOORE, M. MORII, B. MOURS, D. MULLER, G. MUELLER, V. MURZIN, T. NAGAMINE, S. NARITA, U. NAUENBERG, H. NEAL, G. NESOM, M. NUSSBAUM, Y. OHNISHI, N. OISHI, D. ONOPRIENKO, L.S. OSBORNE, R.S. PANVINI, C.H.PARK, H. PARK, T.J. PAVEL, I. PERUZZI, L. PESCARA, M. PICCOLO, L. PIEMONTESE, E. PIERONI, K.T. PITTS, R.J. PLANO, R. PREPOST, C.Y. PRESCOTT, G. PUNKAR, J. QUIGLEY, B.N. RATCLIFF, K. REEVES, T.W. REEVES, J. REIDY, P.L. REINERTSEN, P.E. RENSING, L.S. ROCHESTER, J.E. ROTHBERG, P.C. ROWSON, J.J. RUSSELL, O.H. SAXTON, T. SCHALK, R.H. SCHINDLER, U. SCHNEEKLOTH, B.A. SCHUMM, J. SCHWIENING, A. SEIDEN, S. SEN, V.V. SERBO, L. SERVOLI, M.H. SHAEVITZ, J.T. SHANK, G. SHAPIRO, D.J. SHERDEN, K.D. SHMAKOV, C. SIMOPOULOS, N.B. SINEV, S.R. SMITH, M.B. SMY, J.A. SNYDER, M.D. SOKOLOFF, H. STAENGLE, A. STAHL, P. STAMER, H. STEINER, R. STEINER, M.G. STRAUSS, D. SU, F. SUEKANE, A. SUGIYAMA, A. SUZUKI, S. SUZUKI, M. SWARTZ, A. SZUMILO, T. TAKAHASHI, F.E. TAYLOR, J.J. THALER, J. THOM, E. TORRENCE, A.I. TRANDAFIR, J.D. TURK, T. USHER, J. VA VRA, C. VANNINI, E. VELLA, J.P. VENUTI, R. VERDIER, P.G. VERDINI, D.L. WAGNER, S.R. WAGNER, A.P. WAITE, S. WALSTON, J. WANG, S.J. WATTS, A.W. WEIDEMANN, E.R. WEISS, J.S. WHITAKER, S.L. WHITE, F.J. WICKENS, D.A. WILLIAMS, D.C. WILLIAMS, S.H. WILLIAMS, S. WILLOCQ, R.J. WILSON, W.J. WISNIEWSKI, J.L. WITTLIN, M. WOODS, G.B. WORD, T.R. WRIGHT, J. WYSS, R.K. YAMAMOTO, J.M. YAMARTINO, X.Q. YANG, J. YASHIMA, S.J. YELLIN, C.C.YOUNG, H.YUTA, G. ZAPALAC, R.W. ZDARKO, C. ZEITLIN, J. ZHOU, Centre National de la Recherche Scientifique (CNRS)-École polytechnique (X)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3), Centre National de la Recherche Scientifique (CNRS)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université Paris-Sud - Paris 11 (UP11), Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université Pierre et Marie Curie - Paris 6 (UPMC), Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut Polytechnique de Grenoble - Grenoble Institute of Technology-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3), Laboratoire d'Annecy de Physique des Particules (LAPP/Laboratoire d'Annecy-le-Vieux de Physique des Particules), Centre National de la Recherche Scientifique (CNRS)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3), Centre National de la Recherche Scientifique (CNRS)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Aix Marseille Université (AMU), Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Institut Polytechnique de Grenoble - Grenoble Institute of Technology-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Collège de France (CdF)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), GRUNEVALD M, NEWALD M, SCHAEL S, BARATE R, BRUNELIE, RE R, BUSKULIC D, DE BONIS I, DECAMP D, GHEZ P, GOY C, JE, ZE, QUEL S, LEES J, LUCOTTE A, MARTIN M, MERLE E, MINARD M, NIEF J, ODIER P, PIETRZYK B, TROCME, BRAVO S, CASADO MP, CHMEISSANI M, COMAS P, CRESPO JM, FERNANDEZ E, FERNANDEZ-BOSMAN M, GARRIDO L, GRAUGES E, JUSTE A, MARTINEZ M, MERINO G, MIQUEL R, MIR LM, ORTEU S, PACHECO A, PARK IC, PERLAS J, RIU I, RUIZ H, SANCHEZ F, COLALEO A, CREANZA D, DE FILIPPIS N, DE PALMA M, IASELLI G, MAGGI G, MAGGI M, NUZZO S, RANIERI, A, RASO, G, RUGGIERI F, SELVAGGI, G, SILVESTRIS L, TEMPESTA P, TRICOMI A, ZITO G, HUANG X, LIN J, OUYANG Q, WANG T, XIE Y, XU R, XUE S, ZHANG J, ZHANG L, ZHAO W, ABBANEO D, BAZARKO A, BECKER U, BOIX G, BIRD F, BLUCHER E, BONVICINI B, BRIGHT-THOMAS P, BARKLOW T, BUCHMU, LLER O, CATTANEO M, CERUTTI F, CIULLI V, CLERBAUX B, DREVERMANN H, FORTY RW, FRANK M, GREENING TC, HAGELBERG R, HALLEY AW, GIANOTTI F, GIRONE M, HANSEN JB, HARVEY J, JACOBSEN J, HUTCHCROFT DE, JANOT P, JOST B, KNOBLOCH J, KADO M, LEHRAUS I, LAZEYRAS P, and MALEY P
- Subjects
Top quark ,FORWARD-BACKWARD ASYMMETRY ,PARTICLE PHYSICS ,LARGE ELECTRON POSITRON COLLIDER ,ALEPH ,DELPHI ,L3 ,OPAL ,General Physics and Astronomy ,01 natural sciences ,7. Clean energy ,High Energy Physics - Experiment ,Settore FIS/04 - Fisica Nucleare e Subnucleare ,High Energy Physics - Experiment (hep-ex) ,High Energy Physics - Phenomenology (hep-ph) ,electron-positron physics ,[PHYS.HEXP]Physics [physics]/High Energy Physics - Experiment [hep-ex] ,Electroweak interaction ,Physics ,Quantum chromodynamics ,Electron–positron physics ,Electroweak interactions ,Decays of heavy intermediate gauge bosons ,Fermion–antifermion production ,Precision measurements at the Z resonance ,Tests of the Standard Model ,Radiative corrections ,Effective coupling constants ,Neutral weak current ,Z boson ,W boson ,Higgs boson ,Particle physics - Experiment ,Settore FIS/01 - Fisica Sperimentale ,FERMION-PAIR PRODUCTION ,HADRONIC-Z-DECAYS ,TOP-QUARK MASS ,ANGLE BHABHA SCATTERING ,W-BOSON MASS ,CROSS-SECTION ASYMMETRY ,Z-LINE-SHAPE ,SEMILEPTONIC BRANCHING RATIOS ,CARLO EVENT GENERATOR ,decays of heavy intermediate gauge bosons ,effective coupling constants ,electroweak interactions ,fermion-antifermion production ,higgs boson ,neutral weak current ,precision measurements at the z resonance ,radiative corrections ,tests of the standard model ,top quark ,w boson ,z boson ,Radiative correction ,High Energy Physics - Phenomenology ,FIS/01 - FISICA SPERIMENTALE ,Física nuclear ,Neutrino ,Particle physics ,FOS: Physical sciences ,ddc:500.2 ,Elementary particle physics ,LEP ,electroweak ,Decays of heavy intermediate gauge boson ,Effective coupling constant ,Partícules (Física nuclear) ,Standard Model ,electroweak theory, Z boson, DELPHI, ALEPH, OPAL, L3 ,0103 physical sciences ,010306 general physics ,Coupling constant ,010308 nuclear & particles physics ,High Energy Physics::Phenomenology ,Fermion ,FORWARD-BACKWARD ASYMMETRY, FERMION-PAIR PRODUCTION, HADRONIC-Z-DECAYS, TOP-QUARK MASS, ANGLE BHABHA SCATTERING, W-BOSON MASS, CROSS-SECTION ASYMMETRY, Z-LINE-SHAPE, SEMILEPTONIC BRANCHING RATIOS, CARLO EVENT GENERATOR ,[PHYS.HPHE]Physics [physics]/High Energy Physics - Phenomenology [hep-ph] ,Experimental High Energy Physics ,Electron–positron physic ,High Energy Physics::Experiment ,FIS/04 - FISICA NUCLEARE E SUBNUCLEARE - Abstract
We report on the final electroweak measurements performed with data taken at the Z resonance by the experiments operating at the electron-positron colliders SLC and LEP. The data consist of 17 million Z decays accumulated by the ALEPH, DELPHI, L3 and OPAL experiments at LEP, and 600 thousand Z decays by the SLD experiment using a polarised beam at SLC. The measurements include cross-sections, forward-backward asymmetries and polarised asymmetries. The mass and width of the Z boson, $\MZ$ and $\GZ$, and its couplings to fermions, for example the $\rho$ parameter and the effective electroweak mixing angle, are precisely measured. The number of light neutrino species is determined to be 2.9840+/-0.0082. The results are compared to the predictions of the Standard Model. Electroweak radiative corrections beyond the running of the QED and QCD coupling constants are observed with a significance of five standard deviations, and in agreement with the Standard Model. Of the many Z-pole measurements, the forward-backward asymmetry in b-quark production shows the largest difference with respect to its Standard Model expectation, at the level of 2.8 standard deviations. Through radiative corrections evaluated in the framework of the Standard Model, the masses of the top quark and the W Boson are predicted. These indirect constraints are compared to the direct measurements, providing a stringent test of the Standard Model. Using in addition the direct measurements of $\Mt$ and $\MW$, the mass of the as yet unobserved Standard Model Higgs boson is predicted., Comment: 302 pages, v2: minor corrections and updates of references. Accepted for publication by Physics Reports, v3: further small corrections and journal version
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- 2006
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9. Effect of the point mutation H148G on GFPmut2 unfolding kinetics by fluorescence spectroscopy
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Bosisio, C, Quercioli, V, Chirico, G, D'Alfonso, L, Bettati, S, Raboni, S, Campanini, B, Collini, M, BOSISIO, CHIARA, Bosisio, C, Quercioli, V, Chirico, G, D'Alfonso, L, Bettati, S, Raboni, S, Campanini, B, Collini, M, and BOSISIO, CHIARA
- Abstract
We have used fluorescence spectroscopy techniques such as fluorescence correlation spectroscopy and fluorescence anisotropy decay on a wide time range, from nanoseconds to seconds, to investigate the unfolding kinetics induced by guanidinium chloride of GFPMut2 and its point mutation H148G, which has proved to be relevant for GFP photochemistry and photophysics. The mutation affects the unfolding kinetics of GFP leading to a much faster process at alkaline pH values, where protonation dynamics is negligible, that can be ascribed to a twofold role of His148, either as a proton shutter towards the chromophore and as a conformation stabiliser. For both mutants a soft region located near beta-strand 3 is found that starts to gain flexibility in the ns range at denaturant concentrations far lower than those required to turn off the chromophore fluorescence, as derived from the anisotropy decay of an extrinsic probe covalently bound to the proteins. © 2011 Elsevier B.V. All rights reserved.
- Published
- 2011
10. Photo-induced Millisecond Switching Kinetics in the GFPMUT2 E222Q Mutant
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Quercioli, V, Bosisio, C, Daglio, S, Rocca, F, D'Alfonso, L, Collini, M, Baldini, G, Chirico, G, Bettati, S, Raboni, S, Campanini, B, DAGLIO, STEFANO CARLO, D'ALFONSO, LAURA, COLLINI, MADDALENA, BALDINI, GIANCARLO, CHIRICO, GIUSEPPE, Campanini, B., Quercioli, V, Bosisio, C, Daglio, S, Rocca, F, D'Alfonso, L, Collini, M, Baldini, G, Chirico, G, Bettati, S, Raboni, S, Campanini, B, DAGLIO, STEFANO CARLO, D'ALFONSO, LAURA, COLLINI, MADDALENA, BALDINI, GIANCARLO, CHIRICO, GIUSEPPE, and Campanini, B.
- Abstract
New probes for kinetic intracellular measurements in the millisecond range are desirable to monitor protein biochemical dynamics essential for catalysis, allosteric regulation and signalling. Good candidates to this aim are the photo-switchable mutants of the Green Fluorescent Protein, whose anionic fluorescence, primed by blue light, is markedly enhanced under an additional excitation at a shorter wavelength and relaxes within few milliseconds. The aim of this report is to study how the brightness enhancement kinetics depends on the physical-chemical and spectroscopic parameters and to provide proof-of-concepts experiments for the use of the fluorescence enhancement in conditions in which the protein diffusion is hindered and thereby photo-bleaching can be a limiting critical issue. Future, direct applications of photo-chromic mutants for modulated excitation imaging would in fact require such a detailed knowledge. We present here an extensive study of the photo-switching mechanism of the E222Q mutant of GFPMut2 (Mut2Q), pumped by visible 488 nm light and probed at 400-420 nm, as a function of pH, viscosity, temperature and light intensity. In solution, two characteristic photo-switching times are found by means of modulated double beam fluorescence correlation spectroscopy in the 1-30 ms range, depending on the solution pH. The photo-switching kinetics is solved in terms of the eigenvalues and the eigenvectors of a specific energy diagram and directly used to fit the data, suggesting that the observed photo-switching amplitudes and kinetics are related to a single three-levels transition loop. Finally we give in-vitro examples of the use of modulated excitation microscopy, based on fluorescence enhancement amplitude and kinetics detection, on Mut2Q protein samples immobilized in acrylamide gels.
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- 2010
11. Protonation and Conformational Dynamics of GFP Mutants by Two-Photon Excitation Fluorescence Correlation Spectroscopy
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Bosisio, C, Quercioli, V, Collini, M, D'Alfonso, L, Baldini, G, Bettati, S, Campanini, B, Raboni, S, Chirico, G, COLLINI, MADDALENA, D'ALFONSO, LAURA, BALDINI, GIANCARLO, CHIRICO, GIUSEPPE, Bosisio, C, Quercioli, V, Collini, M, D'Alfonso, L, Baldini, G, Bettati, S, Campanini, B, Raboni, S, Chirico, G, COLLINI, MADDALENA, D'ALFONSO, LAURA, BALDINI, GIANCARLO, and CHIRICO, GIUSEPPE
- Abstract
GFP, fluorescence, fluctuation spectroscopy, protonation dynamics
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- 2008
12. Triptorelin administration in the luteal phase improves the outcome of art cycles
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Fusi, F.M., primary, Arnoldi, M., additional, Bosisio, C., additional, Zanga, L., additional, and Frigerio, L., additional
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- 2013
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13. prevenzione delle infezioni negli operatori sanitari
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Privitera, GAETANO PIERPAOLO, Auxilia, F, and Bosisio, C.
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- 1997
14. Polymerization of butadiene, 4-methyl-1,3-pentadiene and styrene with catalyst systems based on bis-cyclopentadienyl derivatives of Titanium
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Ricci, G., Bosisio, C., and Porri, L.
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- 1996
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15. Application of clinical audit to the evaluation of prescription of interferon-alpha in a teaching hospital
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Privitera, GAETANO PIERPAOLO, Auxilia, F, Bosisio, C, Castaldi, S, and Pagano, A.
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- 1996
16. PCA1: PHARMACOECONOMIC EVALUATION OF FILGRASTIM (r-metHuG-CSF) TREATMENT IN AUTOLOGOUS BONE MARROW TRANSPLANTATION
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Privitera, G, primary, Della Volpe, A, additional, Tagliaferri, E, additional, Vegni, FE, additional, Bosisio, C, additional, Bottoni, A, additional, Pierfederici, P, additional, Auxilia, F, additional, and Lambertenghi Deliliers, G, additional
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- 1999
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17. Cerebral arterial spasm after subarachnoid hemorrhage. A case report with SPECT and iodine-123 HIPDM
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Di Piero, V, Caneschi, S, Bianchi Bosisio, C, Gerundini, P, Triulzi, F, Perani, D, Fazio, F, FAZIO, FERRUCCIO, Di Piero, V, Caneschi, S, Bianchi Bosisio, C, Gerundini, P, Triulzi, F, Perani, D, Fazio, F, and FAZIO, FERRUCCIO
- Abstract
Cerebral arterial spasm following subarachnoid hemorrhage can be responsible for secondary neurological deficits and can influence history, prognosis, and results of surgical treatment. The demonstration of vasospasm can be important for therapy. The use of I-123 HIPDM and SPECT in evaluating regional cerebral blood flow showed the effects on cerebral perfusion of diffuse arterial spasm in a case of subarachnoid hemorrhage and its recovery. There was a parallel with clinical neurological status
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- 1987
18. NEUROACTIVE DRUGS IN ENDOCRINE DISEASES - NOTES ON THEIR PHYSIOPATHOLOGICAL AND THERAPEUTIC ASPECTS
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Silvestrini, Gf, Gelli, D., Liuzzi, A., Chiodini, Pg, Cozzi, R., Loli, R., Verde, G., Beretta, A., Bonomo, M., Bosisio, C., Colombo, G., Cremoncini, M., Derisi, A., Favales, F., Frascatani, F., Goi, D., Maggioni, D., Mauri, R., Filippo Muratori, Perrino, L., Ravanelli, M., Riolo, A., Ronzoni, M., Spelta, B., Valente, C., Botalla, L., and Beltrame, S.
19. Application of clinical audit to the evaluation of prescription of interferon-alpha in a teaching hospital | Applicazione di una procedura di audit per la valutazione della prescrizione di interferone-alfa in un ospedale di insegnamento
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GAETANO PIERPAOLO PRIVITERA, Auxilia, F., Bosisio, C., Castaldi, S., and Pagano, A.
20. Evaluation of the results of the application of personnel assessment methods to the supply of nursing personnel at the hospital level. A case study carried out at th I.R.C.C.S.--Ospedale Maggiore di Milano (Greater Hospital of Milan) | Valutazione dei risultati della applicazione di metodologie di definizione delle dotazioni organiche di personale infermieristico in ambito ospedaliero. Caso di studio presso l'I.R.C.C.S.--Ospedale Maggiore di Milano
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Bosisio, C., Castaldi, S., Magistrelli, V., Regazzo, C., and francesco auxilia
21. Cerebral Arterial Spasm after Subarachnoid Hemorrhage A Case Report with SPECT and lodine-123 HIPDM
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PIERO, V D, primary, CANESCHI, S, additional, BOSISIO, C BIANCHI, additional, GERUNDINI, P, additional, TRIULZI, F, additional, PERANI, D, additional, and FAZIO, F, additional
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- 1987
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22. Long-Term Mental and Physical Quality of Life after Radical and Conservative Surgeries and in Relation to Childbirth in Patients with Severe Endometriosis
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Angelo Compare, Apollonia Verrengia, Gaetano Trezzi, Luigi Frigerio, Enrico Betto, Chiara Bosisio, Chiara Malandrino, Massimo Rabboni, Cristina Zarbo, Rita Secomandi, Betto, E, Zarbo, C, Verrengia, A, Malandrino, C, Secomandi, R, Trezzi, G, Bosisio, C, Rabboni, M, Compare, A, and Frigerio, L
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Quality of life ,medicine.medical_specialty ,Endometriosis ,Vitality ,03 medical and health sciences ,0302 clinical medicine ,Settore M-PSI/08 - Psicologia Clinica ,medicine ,Severe endometriosis ,Childbirth ,In patient ,030212 general & internal medicine ,Radical surgery ,Endometriosi ,030219 obstetrics & reproductive medicine ,business.industry ,medicine.disease ,Bodily pain ,Physical therapy ,Surgery ,Mental health ,business - Abstract
Aim: This study aimed to investigate which surgery (conservative, radical) is the most effective in improving quality of life and if childbirth after conservative one affects outcomes. Method: 79 women who underwent radical or conservative surgery for severe endometriosis were assessed by means of a semistructured interview and completed the SF-36. Results: Our findings showed that in the whole model of Hierarchical multiple linear regression analyses, surgery group explained significantly 10.7% of variance for bodily pain, 7.2% for general health, and 7.9% for vitality, independently of age and months spent since surgery. Groups of childbirth differed significantly for vitality (p .018, Cohen’s d .939) and role emotional (p .034, Cohen’s d .786). Discussion: Findings suggested that women with endometriosis who underwent radical surgery showed better quality of life in long term, independently of age and time spent since treatment. Moreover, women who gave birth to a child had a better mental quality of life, especially concerning vitality and role limitations due to emotional problems.
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- 2017
23. Cerebral arterial spasm after subarachnoid hemorrhage. A case report with SPECT and iodine-123 HIPDM
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V. Di Piero, S. Caneschi, Ferruccio Fazio, Paolo Gerundini, C. Bianchi Bosisio, Fabio Triulzi, Daniela Perani, Di Piero, V, Caneschi, S, Bianchi Bosisio, C, Gerundini, P, Triulzi, F, Perani, D, Fazio, F, Di Piero, V., Caneschi, S., Bianchi Bosisio, C., Gerundini, P., Triulzi, F., Perani, DANIELA FELICITA L., and Fazio, F.
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Subarachnoid hemorrhage ,Arterial spasm ,Iodine-123 ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,cardiovascular diseases ,Cerebral perfusion pressure ,Radionuclide Imaging ,business.industry ,Iodobenzenes ,Neurological status ,fungi ,food and beverages ,Vasospasm ,General Medicine ,Subarachnoid Hemorrhage ,Middle Aged ,medicine.disease ,nervous system diseases ,Cerebral blood flow ,Ischemic Attack, Transient ,Anesthesia ,Iodobenzene ,Female ,business ,Tomography, X-Ray Computed ,Cerebral arterial spasm ,Human - Abstract
Cerebral arterial spasm following subarachnoid hemorrhage can be responsible for secondary neurological deficits and can influence history, prognosis, and results of surgical treatment. The demonstration of vasospasm can be important for therapy. The use of I-123 HIPDM and SPECT in evaluating regional cerebral blood flow showed the effects on cerebral perfusion of diffuse arterial spasm in a case of subarachnoid hemorrhage and its recovery. There was a parallel with clinical neurological status.
- Published
- 1987
24. Semplificazione e redazione scientifica ad alta diffusione: un'analisi dei foglietti illustrativi delle specialità medicinali
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BERSANI BERSELLI, GABRIELE, BOSISIO C., CAVAGNOLI S., and BERSANI BERSELLI G.
- Subjects
Generi testuali ,SEMPLIFICAZIONE ,LINGUAGGI SPECIALISTICI - Abstract
While text simplification is a main strategy to improve readability, it seems severely constrained by the choice of text genre. In a discourse community, such those involving LSPs, but not only, there are expert members who establish the rules of style, content, addressees of texts. The point is here discussed with reference to the sub-genre of (consumer) medicine information leaflets, comparing a “standard” leaflet with a simplified one.
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- 2013
25. Effect of the point mutation H148G on GFPmut2 unfolding kinetics by fluorescence spectroscopy
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Valentina Quercioli, Stefano Bettati, Giuseppe Chirico, Barbara Campanini, Laura D'Alfonso, Maddalena Collini, Samanta Raboni, Chiara Bosisio, Bosisio, C, Quercioli, V, Chirico, G, D'Alfonso, L, Bettati, S, Raboni, S, Campanini, B, Collini, M, Dipartimento di Fisica, Università degli Studi di Milano-Bicocca [Milano] (UNIMIB), Dipartimento di Biochimica e Biologia Molecolare, University of Parma = Università degli studi di Parma [Parme, Italie], and Istituto Nazionale di Biostrutture e Biosistemi
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Guanidinium chloride ,Protein Denaturation ,Protein Conformation ,Glutamine ,Kinetics ,Green Fluorescent Proteins ,Biophysics ,GFPmut2) ,Fluorescence correlation spectroscopy ,fluorescence correlation spectroscopy ,Unfolding ,Photochemistry ,GFP ,Green Fluorescent Protein ,Biochemistry ,Fluorescence spectroscopy ,Green fluorescent protein ,03 medical and health sciences ,chemistry.chemical_compound ,Point Mutation ,Histidine ,Amino Acids ,030304 developmental biology ,Fluorescence correlation spectroscopy (H148G, GFPmut2) ,Protein Unfolding ,Kinetic ,0303 health sciences ,Protein Stability ,030302 biochemistry & molecular biology ,Organic Chemistry ,Chromophore ,Fluorescence ,Amino Acid ,Spectrometry, Fluorescence ,chemistry ,Biophysic ,[PHYS.PHYS.PHYS-CHEM-PH]Physics [physics]/Physics [physics]/Chemical Physics [physics.chem-ph] ,(H148G ,Fluorescence anisotropy - Abstract
We have used fluorescence spectroscopy techniques such as fluorescence correlation spectroscopy and fluorescence anisotropy decay on a wide time range, from nanoseconds to seconds, to investigate the unfolding kinetics induced by guanidinium chloride of GFPMut2 and its point mutation H148G, which has proved to be relevant for GFP photochemistry and photophysics. The mutation affects the unfolding kinetics of GFP leading to a much faster process at alkaline pH values, where protonation dynamics is negligible, that can be ascribed to a twofold role of His148, either as a proton shutter towards the chromophore and as a conformation stabiliser. For both mutants a soft region located near beta-strand 3 is found that starts to gain flexibility in the ns range at denaturant concentrations far lower than those required to turn off the chromophore fluorescence, as derived from the anisotropy decay of an extrinsic probe covalently bound to the proteins. © 2011 Elsevier B.V. All rights reserved.
- Published
- 2011
- Full Text
- View/download PDF
26. Photoinduced millisecond switching kinetics in the GFPMut2 E222Q mutant
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Maddalena Collini, Samanta Raboni, Barbara Campanini, Francesco Rocca, Stefano Carlo Daglio, Giancarlo Baldini, Stefano Bettati, Chiara Bosisio, Valentina Quercioli, Laura D'Alfonso, Giuseppe Chirico, Quercioli, V, Bosisio, C, Daglio, S, Rocca, F, D'Alfonso, L, Collini, M, Baldini, G, Chirico, G, Bettati, S, Raboni, S, and Campanini, B
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Time Factors ,Kinetics ,Green Fluorescent Proteins ,Analytical chemistry ,2-Color ,FIS/07 - FISICA APPLICATA (A BENI CULTURALI, AMBIENTALI, BIOLOGIA E MEDICINA) ,fluorescence correlation spectroscopy ,Fluorescence correlation spectroscopy ,02 engineering and technology ,GFP ,010402 general chemistry ,Green Fluorescent Protein ,01 natural sciences ,Catalysis ,Photochromism ,dual color excitation ,Microscopy ,Materials Chemistry ,Physical and Theoretical Chemistry ,Silica-Gel ,Excitation ,Millisecond ,Photobleaching ,Chemistry ,fluorescence enhancement ,Hydrogen-Ion Concentration ,021001 nanoscience & nanotechnology ,Fluorescence ,Single-Molecule Spectroscopy ,0104 chemical sciences ,Surfaces, Coatings and Films ,Light intensity ,GFP Mutant ,Spectrometry, Fluorescence ,Amino Acid Substitution ,Biophysics ,Mutagenesis, Site-Directed ,Autofluorescent Protein ,Conformational Dynamic ,Resolution ,0210 nano-technology - Abstract
New probes for kinetic intracellular measurements in the millisecond range are desirable to monitor protein biochemical dynamics essential for catalysis, allosteric regulation and signalling. Good candidates to this aim are the photo-switchable mutants of the Green Fluorescent Protein, whose anionic fluorescence, primed by blue light, is markedly enhanced under an additional excitation at a shorter wavelength and relaxes within few milliseconds. The aim of this report is to study how the brightness enhancement kinetics depends on the physical-chemical and spectroscopic parameters and to provide proof-of-concepts experiments for the use of the fluorescence enhancement in conditions in which the protein diffusion is hindered and thereby photo-bleaching can be a limiting critical issue. Future, direct applications of photo-chromic mutants for modulated excitation imaging would in fact require such a detailed knowledge. We present here an extensive study of the photo-switching mechanism of the E222Q mutant of GFPMut2 (Mut2Q), pumped by visible 488 nm light and probed at 400-420 nm, as a function of pH, viscosity, temperature and light intensity. In solution, two characteristic photo-switching times are found by means of modulated double beam fluorescence correlation spectroscopy in the 1-30 ms range, depending on the solution pH. The photo-switching kinetics is solved in terms of the eigenvalues and the eigenvectors of a specific energy diagram and directly used to fit the data, suggesting that the observed photo-switching amplitudes and kinetics are related to a single three-levels transition loop. Finally we give in-vitro examples of the use of modulated excitation microscopy, based on fluorescence enhancement amplitude and kinetics detection, on Mut2Q protein samples immobilized in acrylamide gels.
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- 2010
27. Il nonprofit come erogatore di servizi pubblici in Italia e negli Stati Uniti: bandi di finanziamento e requests for proposals a confronto
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FUSARI, SABRINA, Bosisio C, Cambiaghi B, Piemontese E, Santulli F, and FUSARI S
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NONPROFIT ,INTERCULTURAL COMMUNICATION ,CORPUS LINGUISTICS ,DISCOURSE ANALYSIS - Abstract
The language of nonprofit organizations has originated considerable interest among applied linguists in recent years, mainly with reference to persuasive discourse for fund raising purposes. These studies, which combine genre analysis with corpus linguistics, have led to the creation of two corpora, the ICIC Fundraising Corpus, developed at Indiana University-Purdue University at Indianapolis (USA), and the SITLEC Fundraising Corpus, developed at the University of Bologna at Forlì. This contribution aims to extend the focus of these studies beyond fundraising organizations, to include grantmaking foundations. The analysis is focused on two requests for proposals, concerning medical research, one by Fondazione CARIPLO, and one by the Bill & Melinda Gates Foundation, which are compared from the structural viewpoints.
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- 2008
28. Retorica del notiziario nell'Italia contemporanea
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MICHELE LOPORCARO, Cristina Bosisio, Bona Cambiaghi, Emanuela Piemontese, Francesca Santulli, Loporcaro, Michele, University of Zurich, Bosisio, C, Cambiaghi, B, Piemontese, E, Santulli, F, and Loporcaro, M
- Subjects
470 Latin & Italic languages ,460 Spanish & Portuguese languages ,410 Linguistics ,450 Italian, Romanian & related languages ,800 Literature, rhetoric & criticism ,Settore L-LIN/01 - Glottologia e Linguistica ,440 French & related languages ,10103 Institute of Romance Studies - Published
- 2008
29. Protonation and Conformational Dynamics of GFP Mutants by Two-Photon Excitation Fluorescence Correlation Spectroscopy
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Valentina Quercioli, Maddalena Collini, Samanta Raboni, Stefano Bettati, Chiara Bosisio, Barbara Campanini, Laura D'Alfonso, Giuseppe Chirico, Giancarlo Baldini, Bosisio, C, Quercioli, V, Collini, M, D'Alfonso, L, Baldini, G, Bettati, S, Campanini, B, Raboni, S, and Chirico, G
- Subjects
Threonine ,Protein Conformation ,Green Fluorescent Proteins ,Glutamic Acid ,Fluorescence correlation spectroscopy ,Protonation ,Photochemistry ,Green fluorescent protein ,Serine ,Protein structure ,Materials Chemistry ,GFP, protonation dynamics, fluorescence, fluctuation spectroscopy ,Computer Simulation ,Histidine ,Physical and Theoretical Chemistry ,Photons ,Luminescent Agents ,Chemistry ,Chromophore ,Hydrogen-Ion Concentration ,Fluorescence ,Surfaces, Coatings and Films ,Spectrometry, Fluorescence ,Mutation ,Protons - Abstract
GFP mutants are known to display fluorescence flickering, a process that occurs in a wide time range. Because serine 65, threonine 203, glutamate 222, and histidine 148 have been indicated as key residues in determining the GFP fluorescence photodynamics, we have focused here on the role of histidine 148 and glutamate 222 by studying the fluorescence dynamics of GFPmut2 (S65A, V68L, and S72A GFP) and its H148G (Mut2G) and E222Q (Mut2Q) mutants. Two relaxation components are found in the fluorescence autocorrelation functions of GFPmut2: a 10-100 micros pH-dependent component and a 100-500 micros laser-power-dependent component. The comparison of these three mutants shows that the mutation of histidine 148 to glycine induces a 3-fold increase in the protonation rate, thereby indicating that the protonation-deprotonation of the chromophore occurs via a proton exchange with the solution mediated by the histidine 148 residue. The power-dependent but pH-independent relaxation mode, which is not affected by the E222Q and H148G mutations, is due to an excited-state process that is probably related to conformational rearrangements of the chromophore after the photoexcitation, more than to the chromophore excited-state proton transfer.
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- 2008
30. SuperB: A High-Luminosity Asymmetric e+ e- Super Flavor Factory. Conceptual Design Report
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M. BONA, J. GARRA TIC´O, E. GRAUG´ES POUS, P. COLANGELO, F. DE FAZIO, A. PALANO, M. MANGHISONI, V. RE, G. TRAVERSI, M. BRUSCHI, B. GIACOBBE, R. SPIGHI, A. Y. BARNIAKOV, M. Y. BARNIAKOV, V. E. BLINOV, V. P. DRUZHININ, V. B. GOLUBEV, S. A. KONONOV, I. A. KOOP, E. A. KRAVCHENKO, E. B. LEVICHEV, S. A. NIKITIN, A. P. ONUCHIN, P. A. PIMINOV, S. I. SEREDNYAKOV, D. N. SHATILOV, Y. M. SHATUNOV, Y. I. SKOVPEN, E. P. SOLODOV, C. H. CHENG, B. ECHENARD, F. FANG, D. G. HITLIN, F. C. PORTER, R. FLEISCHER, G. F. GIUDICE, T. HURTH, M. MANGANO, G. MANCINELLI, B. T. MEADOWS, A. J. SCHWARTZ, M. D. SOKOLOFF, T. HAAS, R. MANKEL, P. BALL, M. PAPPAGALLO, M. R. PENNINGTON, W. GRADL, S. PLAYFER, A. ABADA, D. BECIREVIC, S. DESCOTES GENON, O. P`ENE, D. ANDREOTTI, M. ANDREOTTI, D. BETTONI, C. BOZZI, R. CALABRESE, A. CECCHI, G. CIBINETTO, P. FRANCHINI, E. LUPPI, M. NEGRINI, A. PETRELLA, L. PIEMONTESE, E. PRENCIPE, V. SANTORO, G. STANCARI, F. ANULLI, R. BALDINI FERROLI, M. E. BIAGINI, M. BOSCOLO, A. CALCATERRA, A. DRAGO, G. FINOCCHIARO, S. GUIDUCCI, G. ISIDORI, S. PACETTI, P. PATTERI, I. M. PERUZZI, M. PICCOLO, M. A. PREGER, P. RAIMONDI, M. RAMA, C. VACCAREZZA, A. ZALLO, M. ZOBOV, R. DE SANGRO, A. BUZZO, M. LO VETERE, M. MACR´, 305, M. R. MONGE, S. PASSAGGIO, C. PATRIGNANI, E. ROBUTTI, S. TOSI, A. LAZZARO, F. PALOMBO, G. BUCHALLA, G. D’AMBROSIO, G. RICCIARDI, I. BIGI, C. P. JESSOP, J. M. LOSECCO, N. ARNAUD, R. CHEHAB, Y. FEDALA, F. POLCI, P. ROUDEAU, V. SORDINI, V. SOSKOV, A. STOCCHI, A. VARIOLA, A. VIVOLI, G. WORMSER, F. ZOMER, A. BERTOLIN, R. BRUGNERA, N. GAGLIARDI, A. GAZ, M. MARGONI, M. MORANDIN, M. POSOCCO, M. ROTONDO, F. SIMONETTO, R. STROILI, G. CALDERINI, L. RATTI, V. SPEZIALI, M. BIASINI, R. COVARELLI, E. MANONI, L. SERVOLI, C. ANGELINI, G. BATIGNANI, S. BETTARINI, F. BOSI, M. CARPINELLI, R. CENCI, A. CERVELLI, M. DELL’ORSO, F. FORTI, P. GIANNETTI, M. GIORGI, A. LUSIANI, G. MARCHIORI, M. MASSA, M. A. MAZUR, F. MORSANI, N. NERI, E. PAOLONI, F. RAFFAELLI, G. RIZZO, J. WALSH, E. BARACCHINI, F. BELLINI, G. CAVOTO, A. D’ORAZIO, D. DEL RE, E. DI MARCO, R. FACCINI, F. FERRAROTTO, M. GASPERO, P. JACKSON, G. MARTINELLI, M. A. MAZZONI, S. MORGANTI, G. PIREDDA, F. RENGA, L. SILVESTRINI, C. VOENA, L. CATANI, A. DI CIACCIO, R. MESSI, E. SANTOVETTI, A. SATTA, M. CIUCHINI, V. LUBICZ, X. CHEN, H. LIU, W. PARK, M. PUROHIT, A. TRIVEDI, R. M. WHITE, J. R. WILSON, M. T. ALLEN, D. ASTON, R. BARTOLDUS, S. J. BRODSKY, Y. CAI, J. COLEMAN, M. R. CONVERY, S. DEBARGER, J. C. DINGFELDER, G. P. DUBOIS FELSMANN, S. ECKLUND, A. S. FISHER, G. HALLER, S. A. HEIFETS, J. KAMINSKI, M. H. KELSEY, M. L. KOCIAN, D. W. G. S. LEITH, N. LI, S. LUITZ, V. LUTH, D. MACFARLANE, R. MESSNER, D. R. MULLER, Y. NOSOCHKOV, A. NOVOKHATSKI, M. PIVI, B. N. RATCLIFF, A. ROODMAN, J. SCHWIENING, J. SEEMAN, A. SNYDER, M. SULLIVAN, J. VA’VRA, U. WIENANDS, W. WISNIEWSKI, F. BIANCHI, D. GAMBA, P. GAMBINO, F. MARCHETTO, E. MENICHETTI, R. MUSSA, M. PELLICCIONI, G. F. DALLA BETTA, M. BOMBEN, L. BOSISIO, C. CARTARO, L. LANCERI, L. VITALE, V. AZZOLINI, J. BERNABEU, N. LOPEZ MARCH, F. MARTINEZ VIDAL, D. A. MILANES, A. OYANGUREN, P. PARADISI, A. PICH, M. A. SANCHIS LOZANO, R. KOWALEWSKI, J. M. RONEY, J. BACK, T. J. GERSHON, P. F. HARRISON, T. E. LATHAM, G. B. MOHANTY, M. PIERINI, DE CASTRO, STEFANO, FACCIOLI, PIETRO, GABRIELLI, ALESSANDRO, SEMPRINI CESARI, NICOLA, VILLA, MAURO, ZOCCOLI, ANTONIO, M. BONA, J. GARRA TIC´O, E. GRAUG´ES POUS, P. COLANGELO, F. DE FAZIO, A. PALANO, M. MANGHISONI, V. RE, G. TRAVERSI, M. BRUSCHI, S. DE CASTRO, P. FACCIOLI, A. GABRIELLI, B. GIACOBBE, N. SEMPRINI CESARI, R. SPIGHI, M. VILLA, A. ZOCCOLI, A.Y. BARNIAKOV, M.Y. BARNIAKOV, V.E. BLINOV, V.P. DRUZHININ, V.B. GOLUBEV, S.A. KONONOV, I.A. KOOP, E.A. KRAVCHENKO, E.B. LEVICHEV, S.A. NIKITIN, A.P. ONUCHIN, P.A. PIMINOV, S.I. SEREDNYAKOV, D.N. SHATILOV, Y.M. SHATUNOV, Y.I. SKOVPEN, E.P. SOLODOV, C.-H. CHENG, B. ECHENARD, F. FANG, D.G. HITLIN, F.C. PORTER, R. FLEISCHER, G.F. GIUDICE, T. HURTH, M. MANGANO, G. MANCINELLI, B.T. MEADOWS, A.J. SCHWARTZ, M.D. SOKOLOFF, T. HAAS, R. MANKEL, P. BALL, M. PAPPAGALLO, M.R. PENNINGTON, W. GRADL, S. PLAYFER, A. ABADA, D. BECIREVIC, S. DESCOTES-GENON, O. P`ENE, D. ANDREOTTI, M. ANDREOTTI, D. BETTONI, C. BOZZI, R. CALABRESE, A. CECCHI, G. CIBINETTO, P. FRANCHINI, E. LUPPI, M. NEGRINI, A. PETRELLA, L. PIEMONTESE, E. PRENCIPE, V. SANTORO, G. STANCARI, F. ANULLI, R. BALDINI-FERROLI, M.E. BIAGINI, M. BOSCOLO, A. CALCATERRA, A. DRAGO, G. FINOCCHIARO, S. GUIDUCCI, G. ISIDORI, S. PACETTI, P. PATTERI, I.M. PERUZZI, M. PICCOLO, M.A. PREGER, P. RAIMONDI, M. RAMA, C. VACCAREZZA, A. ZALLO, M. ZOBOV, R. DE SANGRO, A. BUZZO, M. LO VETERE, M. MACR´ı, and M.R. MONGE, S. PASSAGGIO, C. PATRIGNANI, E. ROBUTTI, S. TOSI, A. LAZZARO, F. PALOMBO, G. BUCHALLA, G. D’AMBROSIO, G. RICCIARDI, I. BIGI, C.P. JESSOP, J.M. LOSECCO, N. ARNAUD, R. CHEHAB, Y. FEDALA, F. POLCI, P. ROUDEAU, V. SORDINI, V. SOSKOV, A. STOCCHI, A. VARIOLA, A. VIVOLI, G. WORMSER, F. ZOMER, A. BERTOLIN, R. BRUGNERA, N. GAGLIARDI, A. GAZ, M. MARGONI, M. MORANDIN, M. POSOCCO, M. ROTONDO, F. SIMONETTO, R. STROILI, G. CALDERINI, L. RATTI, V. SPEZIALI, M. BIASINI, R. COVARELLI, E. MANONI, L. SERVOLI, C. ANGELINI, G. BATIGNANI, S. BETTARINI, F. BOSI, M. CARPINELLI, R. CENCI, A. CERVELLI, M. DELL’ORSO, F. FORTI, P. GIANNETTI, M. GIORGI, A. LUSIANI, G. MARCHIORI, M. MASSA, M.A. MAZUR, F. MORSANI, N. NERI, E. PAOLONI, F. RAFFAELLI, G. RIZZO, J. WALSH, E. BARACCHINI, F. BELLINI, G. CAVOTO, A. D’ORAZIO, D. DEL RE, E. DI MARCO, R. FACCINI, F. FERRAROTTO, M. GASPERO, P. JACKSON, G. MARTINELLI, M.A. MAZZONI, S. MORGANTI, G. PIREDDA, F. RENGA, L. SILVESTRINI, C. VOENA, L. CATANI, A. DI CIACCIO, R. MESSI, E. SANTOVETTI, A. SATTA, M. CIUCHINI, V. LUBICZ, X. CHEN, H. LIU, W. PARK, M. PUROHIT, A. TRIVEDI, R.M. WHITE, J.R. WILSON, M.T. ALLEN, D. ASTON, R. BARTOLDUS, S.J. BRODSKY, Y. CAI, J. COLEMAN, M.R. CONVERY, S. DEBARGER, J.C. DINGFELDER, G.P. DUBOIS-FELSMANN, S. ECKLUND, A.S. FISHER, G. HALLER, S.A. HEIFETS, J. KAMINSKI, M.H. KELSEY, M.L. KOCIAN, D.W.G.S. LEITH, N. LI, S. LUITZ, V. LUTH, D. MACFARLANE, R. MESSNER, D.R. MULLER, Y. NOSOCHKOV, A. NOVOKHATSKI, M. PIVI, B.N. RATCLIFF, A. ROODMAN, J. SCHWIENING, J. SEEMAN, A. SNYDER, M. SULLIVAN, J. VA’VRA, U. WIENANDS, W. WISNIEWSKI, F. BIANCHI, D. GAMBA, P. GAMBINO, F. MARCHETTO, E. MENICHETTI, R. MUSSA, M. PELLICCIONI, G.F. DALLA BETTA, M. BOMBEN, L. BOSISIO, C. CARTARO, L. LANCERI, L. VITALE, V. AZZOLINI, J. BERNABEU, N. LOPEZ-MARCH, F. MARTINEZ-VIDAL, D.A. MILANES, A. OYANGUREN, P. PARADISI, A. PICH, M.A. SANCHIS-LOZANO, R. KOWALEWSKI, J.M. RONEY, J. BACK, T.J. GERSHON, P.F. HARRISON, T.E. LATHAM, G.B. MOHANTY, M. PIERINI
- Subjects
Physics::Instrumentation and Detectors ,Physics::Accelerator Physics ,High Energy Physics::Experiment - Abstract
The physics objectives of SuperB, an asymmetric electron-positron collider with a luminosity above 10^36/cm^2/s are described, together with the conceptual design of a novel low emittance design that achieves this performance with wallplug power comparable to that of the current B Factories, and an upgraded detector capable of doing the physics in the SuperB environment.
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- 2007
31. Letteratura comparata
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SANTANGELO, Giovanni Saverio, MADONIA, Francesco Paolo, RESTUCCIA, Laura, VELEZ, Antonino, Benelli, G, Bogliolo, G, Carile, P, Giorgi, G, Matucci, M, Papasogli, B, Raccanello, M, Colombo Timelli, M, Ferrari, B, Finoli, AM, Conconi, B, Majorano, M, Minerva, N, Bartoccioni, S, Jeronimidis, A, Scaiola, AM, Pompejaono, V, Ciancimino, A, Santangelo, GS, Arcoleo, R, Gagliano, MG, Madonia FP, Restuccia, L, Velez, A, Modenesi, M, Soncini Fratta, A, Gorris, R, Conenna, M, De Gioia, M, Cambiaghi, B, Bosisio, C, Kanceff, E, Santangelo, G., Madonia, F., Restuccia, L., and Velez, A.
- Subjects
Settore L-FIL-LET/14 - Critica Letteraria E Letterature Comparate ,Bibliografia, Letteratura comparata, Italia, Francia - Published
- 2001
32. GnRH agonists to sustain the luteal phase in antagonist IVF cycles: a randomized prospective trial.
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Fusi FM, Brigante CM, Zanga L, Mignini Renzini M, Bosisio C, and Fadini R
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- Adult, Embryo Implantation physiology, Female, Gonadotropin-Releasing Hormone antagonists & inhibitors, Gonadotropin-Releasing Hormone metabolism, Humans, Live Birth, Luteal Phase physiology, Ovarian Hyperstimulation Syndrome diagnosis, Pregnancy, Pregnancy Rate, Progesterone pharmacology, Progestins pharmacology, Prospective Studies, Risk Factors, Triptorelin Pamoate administration & dosage, Embryo Implantation drug effects, Fertilization in Vitro methods, Gonadotropin-Releasing Hormone agonists, Luteal Phase drug effects, Triptorelin Pamoate pharmacology
- Abstract
Background: The addition of a GnRH analogue to the luteal phase in in vitro fertilization programs has been seldom proposed due to the presence of GnRH receptors in the endometrium. The aim of the study was to evaluate the effect of triptorelin addition in short antagonist cycles, compared to cycles where the only supplementation was progesterone., Methods: The primary objective of this study was the study of the effect of Triptorelin addiction during the luteal phase on the live birth rate. Secondary objectives of efficacy were pregnancy rates and implantation rates, as well as safety in terms of OHSS risks. The study was a prospective, randomized, open study, performed in two independent Centers from July 2013 to October 2015. Patients were divided into three groups: a) Regular antagonist protocol, with only luteal progesterone; b) Antagonist protocol with luteal triptorelin as multiple injections, c) Antagonist protocol with luteal triptorelin as single bolus. Descriptive statistics were obtained for all the parameters. Mean and standard deviation were used for all quantitative parameters. Differences between percentages were studied using Chi-square test generalized to the comparison of several proportions., Results: A total number of 1344 patients completed the study, 786 under the age of 35 years, and 558 over 35 years. It was observed an increase of positive HCG results, Clinical pregnancy rates and Delivery rates when triptorelin was added in the luteal phase, irrespective whether as a single bolus or five injections. This increase was statistically significant both for pregnancy rates and delivery rates. The statistic difference between pregnancies and deliveries obtained with or without luteal triptorelin reached p < 0,01. No increase of OHSS risk was observed., Conclusions: From this large study it appears that the concept of luteal phase supplementation should be revisited. From our study it appears that triptorelin addition to the luteal phase of antagonist cycles, either as a single bolus or using multiple injections, is a good tool to optimize ART results., Trial Registration: The study was approved by the Ethics Committee of Provincia di Bergamo (n 1203/2013).
- Published
- 2019
- Full Text
- View/download PDF
33. Safety of ovarian tissue transplantation in patients with borderline ovarian tumors.
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Masciangelo R, Bosisio C, Donnez J, Amorim CA, and Dolmans MM
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- Adult, Animals, Cryopreservation, Female, Humans, Immunohistochemistry, Keratin-7 genetics, Keratin-7 metabolism, Mice, Mice, SCID, Mucin-1 genetics, Mucin-1 metabolism, Ovarian Neoplasms metabolism, Ovary metabolism, Prospective Studies, Reverse Transcriptase Polymerase Chain Reaction, Safety, Transplantation, Heterologous, Fertility Preservation adverse effects, Fertility Preservation methods, Ovarian Neoplasms pathology, Ovarian Neoplasms surgery, Ovary pathology, Ovary transplantation, Tissue Transplantation adverse effects, Tissue Transplantation methods
- Abstract
Study Question: Is transplantation of cryopreserved ovarian tissue from patients with borderline ovarian tumors (BOTs) a safe procedure?, Summary Answer: BOT cells were found in frozen-thawed and xenografted ovarian tissue in 1 of 11 BOT patients., What Is Known Already: The risk of reintroducing malignant cells upon ovarian tissue transplantation has been subject of debate for many years. Reimplantation of cryopreserved ovarian tissue from leukemia patients is unsafe, while results from studies of cryopreserved ovarian tissue from other forms of cancer, such as Hodgkin's lymphoma, are reassuring., Study Design, Size, Duration: Prospective experimental study conducted in an academic research unit using ovarian tissue from 11 patients undergoing cryopreservation for BOTs., Participants/materials, Setting, Methods: Histology, immunohistochemistry (IHC) for mucin 1 (MUC1) and cytokeratin 7 (CK7) and molecular analysis by reverse transcription quantitative polymerase chain reaction (RT-qPCR) for CK7 and MUC1 were performed on frozen-thawed ovarian tissue from 11 patients. Long-term (5 months) xenografting of ovarian tissue in immunodeficient mice was performed. The xenografts were analyzed by histology, IHC and RT-qPCR, furthermore IHC for CD10, a marker of endometriosis, was performed on a selected sample., Main Results and the Role of Chance: Analysis by histology, IHC and RT-qPCR indicated 10 of the ovarian tissue samples were negative. Analysis of the xenograft samples indicated nine were negative for malignant cells but in two xenografts glandular lesions were detected by histology. In these two xenografts, CK7 and MUC1 markers were demonstrated by IHC and CK7 expression also by RT-qPCR. A BOT was confirmed in the xenograft in which the original ovarian tissue was positive, while in the other case IHC demonstrated expression of endometriosis marker CD10., Limitations, Reasons for Caution: Cryopreserved ovarian fragments cannot be tested before transplantation, therefore the preimplantation analysis cannot guarantee that all cryopreserved fragments will be free of BOT cells., Wider Implications of the Findings: BOT cells can be found in cryopreserved ovarian tissue from BOT patients, therefore preimplantation analysis is an absolute prerequisite. Endometriosis can also be detected in cryopreserved ovarian tissue and caution should also be exercised in this scenario., Study Funding/competing Interest(s): This study was supported by grants from the Fonds National de la Recherche Scientifique de Belgique (FNRS-PDR Convention T.0077.14, Télévie Grant 7.4590.16 awarded to Rossella Masciangelo, and Grant 5/4/150/5 awarded to Marie-Madeleine Dolmans), the Fonds Speciaux de Recherche, and the Foundation Against Cancer. None of the authors have any conflicting interests to declare., (© The Author(s) 2017. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com)
- Published
- 2018
- Full Text
- View/download PDF
34. Ovulation induction and luteal support with GnRH agonist in patients at high risk for hyperstimulation syndrome.
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Fusi FM, Arnoldi M, Bosisio C, Lombardo G, Ferrario M, Zanga L, Galimberti A, and Capitanio E
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- Adult, Case-Control Studies, Chorionic Gonadotropin therapeutic use, Female, Fertilization in Vitro, Follicle Stimulating Hormone therapeutic use, Humans, Infertility, Female complications, Luteal Phase, Progesterone therapeutic use, Progestins therapeutic use, Risk, Triptorelin Pamoate therapeutic use, Gonadotropin-Releasing Hormone antagonists & inhibitors, Hormone Antagonists therapeutic use, Hormones therapeutic use, Infertility, Female drug therapy, Luteolytic Agents therapeutic use, Ovarian Hyperstimulation Syndrome epidemiology, Ovulation Induction methods, Polycystic Ovary Syndrome complications, Reproductive Control Agents therapeutic use
- Abstract
The aim of this study was to compare GnRHa trigger and luteal addition of triptorelin to hCG trigger for final oocyte maturation in women at high risk for OHSS undergoing IVF. A total of 423 patients were divided in two groups both stimulated using antagonist short protocol. Gonadotropins 75-150 UI/day were started on day 2-5, GnRH antagonist was added when the lead follicle was >14 mm and the final trigger was obtained with hCG 250 µg or triptorelin 0.2 mg. The luteal phase was supported with progesterone alone in the hCG group, with progesterone plus triptorelin 0.1 every other day from embryo transfer in the triptorelin group. In the triptorelin group we did neither have to suspend any embryo transfer, nor we have any early clinical OHSS. In the control group, 13 patients were suspended due to symptomatic high risk for OHSS and two patients developed a clinically significant OHSS. No statistically significant difference was observed in terms of clinical and ongoing pregnancy rates and implantation rates. Our results indicate that a protocol including GnRHa as trigger and an intensive luteal phase supported with GnRHa is safer than a standard antagonist protocol using hCG as trigger. It displays similar results, therefore it can be used as the first choice in patients at high risk for OHSS.
- Published
- 2015
- Full Text
- View/download PDF
35. DHEA supplementation positively affects spontaneous pregnancies in women with diminished ovarian function.
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Fusi FM, Ferrario M, Bosisio C, Arnoldi M, and Zanga L
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- Adult, Cohort Studies, Drug Administration Schedule, Female, Fertilization in Vitro, Humans, Infertility, Female epidemiology, Infertility, Female etiology, Infertility, Female therapy, Male, Pregnancy, Primary Ovarian Insufficiency complications, Primary Ovarian Insufficiency epidemiology, Dehydroepiandrosterone therapeutic use, Pregnancy Rate, Primary Ovarian Insufficiency therapy, Time-to-Pregnancy drug effects
- Abstract
The aim of this article is to describe unexpected spontaneous pregnancies in poor responder patients with long-term infertility, when treated with dehydroepiandrosterone (DHEA) supplementation prior to in vitro fertilization (IVF). Our evaluation was carried out in two groups of women. The first group included 39 young women with <40 years, all treated with DHEA because of a previous poor response. The second group included 38 women over 40 years who received DHEA supplementation. Controls for latter group were 24 comparable women who had not been treated with DHEA before the first IVF cycle to evaluate the spontaneous pregnancy rate during preparation to IVF. Three tablets daily of 25 mg micronized DHEA were administered for at least 12 weeks before starting a long stimulation protocol for IVF. Surprisingly, spontaneous pregnancy rate significantly increased after DHEA treatment, allowing to achieve 10 spontaneous pregnancies and 9 spontaneous ongoing pregnancies among young poor responders. Pregnancy rate and ongoing pregnancy rate obtained before starting the IVF cycle were also significantly higher in older women treated with DHEA than in the control group: 21.05% and 13.15% and 4.1% and 0, respectively. Our results show that DHEA supplementation improves the ovarian function in poor responders and in women over 40 years, suggesting that this molecule alone can raise fecundity and fertility treatment success in women with poor prognosis for pregnancy.
- Published
- 2013
- Full Text
- View/download PDF
36. Effect of the point mutation H148G on GFPmut2 unfolding kinetics by fluorescence spectroscopy.
- Author
-
Bosisio C, Quercioli V, Chirico G, D'Alfonso L, Bettati S, Raboni S, Campanini B, and Collini M
- Subjects
- Amino Acids, Glutamine metabolism, Green Fluorescent Proteins metabolism, Histidine metabolism, Kinetics, Protein Conformation, Protein Denaturation, Protein Stability, Spectrometry, Fluorescence, Glutamine genetics, Green Fluorescent Proteins chemistry, Green Fluorescent Proteins genetics, Histidine genetics, Point Mutation, Protein Unfolding
- Abstract
We have used fluorescence spectroscopy techniques such as fluorescence correlation spectroscopy and fluorescence anisotropy decay on a wide time range, from nanoseconds to seconds, to investigate the unfolding kinetics induced by guanidinium chloride of GFPMut2 and its point mutation H148G, which has proved to be relevant for GFP photochemistry and photophysics. The mutation affects the unfolding kinetics of GFP leading to a much faster process at alkaline pH values, where protonation dynamics is negligible, that can be ascribed to a twofold role of His148, either as a proton shutter towards the chromophore and as a conformation stabiliser. For both mutants a soft region located near beta-strand 3 is found that starts to gain flexibility in the ns range at denaturant concentrations far lower than those required to turn off the chromophore fluorescence, as derived from the anisotropy decay of an extrinsic probe covalently bound to the proteins., (Copyright © 2011 Elsevier B.V. All rights reserved.)
- Published
- 2011
- Full Text
- View/download PDF
37. Photoinduced millisecond switching kinetics in the GFPMut2 E222Q mutant.
- Author
-
Quercioli V, Bosisio C, Daglio SC, Rocca F, D'Alfonso L, Collini M, Baldini G, Chirico G, Bettati S, Raboni S, and Campanini B
- Subjects
- Amino Acid Substitution, Catalysis, Green Fluorescent Proteins genetics, Green Fluorescent Proteins metabolism, Hydrogen-Ion Concentration, Kinetics, Mutagenesis, Site-Directed, Photobleaching, Spectrometry, Fluorescence, Time Factors, Green Fluorescent Proteins chemistry
- Abstract
New probes for kinetic intracellular measurements in the millisecond range are desirable to monitor protein biochemical dynamics essential for catalysis, allosteric regulation, and signaling. Good candidates to this aim are the photoswitchable mutants of the green fluorescent protein, whose anionic fluorescence, primed by blue light, is markedly enhanced under an additional excitation at a shorter wavelength and relaxes within a few milliseconds. The aim of this report is to study how the brightness enhancement kinetics depends on the physical-chemical and spectroscopic parameters and to provide proof-of-concept experiments for the use of the fluorescence enhancement in conditions in which the protein diffusion is hindered and thereby photobleaching can be a limiting critical issue. Future, direct applications of photochromic mutants for modulated excitation imaging would in fact require such a detailed knowledge. We present here an extensive study of the photoswitching mechanism of the E222Q mutant of GFPMut2 (Mut2Q), pumped by visible 488 nm light and probed at 400-420 nm, as a function of pH, viscosity, temperature, and light intensity. In solution, two characteristic photoswitching times are found by means of modulated double beam fluorescence correlation spectroscopy in the 1-30 ms range, depending on the solution pH. The photoswitching kinetics is solved in terms of the eigenvalues and the eigenvectors of a specific energy diagram and used directly to fit the data, suggesting that the observed photoswitching amplitudes and kinetics are related to a single three-level transition loop. Finally, we give in vitro examples of the use of modulated excitation microscopy, based on fluorescence enhancement amplitude and kinetics detection, on Mut2Q protein samples immobilized in acrylamide gels.
- Published
- 2010
- Full Text
- View/download PDF
38. [Evaluation of the results of the application of personnel assessment methods to the supply of nursing personnel at the hospital level. A case study carried out at th I.R.C.C.S.--Ospedale Maggiore di Milano (Greater Hospital of Milan)].
- Author
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Bosisio C, Castaldi S, Magistrelli V, Regazzo C, and Auxilia F
- Subjects
- Diagnosis-Related Groups, Humans, Italy, Retrospective Studies, Workforce, Hospitalization statistics & numerical data, Hospitals, Teaching, Nursing Staff, Hospital supply & distribution, Personnel Staffing and Scheduling
- Published
- 2000
39. [Application of clinical audit to the evaluation of prescription of interferon-alpha in a teaching hospital].
- Author
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Privitera G, Auxilia F, Bosisio C, Castaldi S, and Pagano A
- Subjects
- Drug Utilization, Hospitals, Teaching, Humans, Italy, Interferon-alpha therapeutic use, Medical Audit
- Abstract
Aim of this study was to evaluate through an audit tool the appropriateness of interferon-alpha (IFN-alpha) prescribing in a teaching hospital. The records of all patients (n.665) treated with IFN-alpha since 1991 were reviewed and the data concerning diagnosis and treatment collected using a standardized from. The data were submitted for peer review to a panel which included the representatives of the different medical specialties which actually prescribed the drug, external experts and members of the health management board of the hospital. The following points were discussed:-adherence to accepted therapeutic indications:-accuracy of the diagnosis prior to treatment:-scheduled doses:-criteria defining non-responders;-length of treatment and criteria for discontinuation. At the end of the study a questionnaire for evaluation of the audit was submitted to all participants. Most patients were treated according to a diagnosis of chronic hepatitis (HCV, n.448; HBV, n.54; HDV, n.22); other indications were chronic myeloid leukemia, thrombocythemia, polycythemia vera, Kaposi's sarcoma, condylomata, melanoma and mycosis fungoides. All indications were approved by the panel: no patient was treated outside established indications (85.6%) or a clinical trial protocol (14.4%). Standard schedules were initially applied to each indication, and were adjusted if needed according to the clinical response. In conclusion, the audit showed that IFN-alpha was correctly prescribed in our teaching hospital; the procedure was well accepted and its application may be useful in modifying prescribing attitudes.
- Published
- 1996
40. Clinical-tomographic evolution of a giant intracranial aneurysm. Case report.
- Author
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Caneschi S, Bianchi Bosisio C, Cazzaniga P, and Mangiagalli E
- Subjects
- Cerebral Angiography, Humans, Intracranial Aneurysm pathology, Male, Middle Aged, Tomography, X-Ray Computed, Intracranial Aneurysm diagnostic imaging
- Abstract
The Authors report about the case of one patient with stroke picture of aphasia and right pyramidal deficiency hemisyndrome evidencing at the CT a picture of multiple bilateral malacic areas associated with a picture of bilateral megadolichosiphon. The later tomographic controls evidenced the occurrence of a right latero-supra-sellar expansive injury compatible with a picture of giant aneurysm of the homolateral carotidal siphon. The Authors analyze the clinical, neuroradiological and anatomopathologic findings in the light of the present publications in the recent literature.
- Published
- 1990
41. Neuroactive drugs in endocrine diseases: notes on their physiopathological and therapeutical aspects.
- Author
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Silvestrini GF, Gelli D, Liuzzi A, Chiodini PG, Cozzi R, Loli R, Verde G, Beretta A, Bonomo M, Bosisio C, Colombo G, Cremoncini M, De Risi A, Favales F, Frascatani F, Goi D, Maggioni D, Mauri R, Muratori F, Perrino L, Ravanelli M, Riolo A, Ronzoni M, Spelta B, and Valente C
- Subjects
- Adrenergic beta-Antagonists therapeutic use, Antipsychotic Agents therapeutic use, Hormones therapeutic use, Humans, Neurosecretion drug effects, Endocrine System Diseases drug therapy
- Published
- 1980
42. [Comparison of high-resolution arterial echography and angiography in the diagnosis of atheromatous lesions of the external carotid].
- Author
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Caneschi S, Bianchi Bosisio C, Frigeni G, Finzi F, Ballabio R, Farina D, and Bertolini A
- Subjects
- Carotid Artery, External diagnostic imaging, Evaluation Studies as Topic, Humans, Radiography, Arteriosclerosis diagnosis, Carotid Artery Diseases diagnosis, Carotid Artery, External pathology, Ultrasonography methods
- Abstract
Personal experience about a correlative study on ultrasonographic and angiographic methods executed on carotid artery is reported. Usual techniques are described and result discordances are considered. Stenosis level is subdivided in four different types: normal, less than 45, 45-75%, greater than 75%. Authors examine in which stenosis range one technique can be superimposed on the other one and show limits and possibilities. Moreover complementary and interdependence position of these two techniques are stressed with respect to atherosclerotic disease study.
- Published
- 1989
43. Cerebral arterial spasm after subarachnoid hemorrhage. A case report with SPECT and iodine-123 HIPDM.
- Author
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Di Piero V, Caneschi S, Bianchi Bosisio C, Gerundini P, Triulzi F, Perani D, and Fazio F
- Subjects
- Female, Humans, Ischemic Attack, Transient etiology, Middle Aged, Radionuclide Imaging, Tomography, X-Ray Computed, Iodobenzenes therapeutic use, Ischemic Attack, Transient diagnostic imaging, Subarachnoid Hemorrhage complications
- Abstract
Cerebral arterial spasm following subarachnoid hemorrhage can be responsible for secondary neurological deficits and can influence history, prognosis, and results of surgical treatment. The demonstration of vasospasm can be important for therapy. The use of I-123 HIPDM and SPECT in evaluating regional cerebral blood flow showed the effects on cerebral perfusion of diffuse arterial spasm in a case of subarachnoid hemorrhage and its recovery. There was a parallel with clinical neurological status.
- Published
- 1987
- Full Text
- View/download PDF
44. [Aspects of diabetes mellitus in our midst; study of 120 female patients].
- Author
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SOUTO MAIOR MC and BOSISIO C
- Subjects
- Female, Humans, Biometry, Diabetes Mellitus epidemiology, Diabetes Mellitus statistics & numerical data, Statistics as Topic
- Published
- 1947
45. [Myocardial fibrosis, arteriosclerosis of the aorta, coronary and cerebral vessels; softening of the bulge; clinico-pathologic session].
- Author
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BOSISIO C and VALLE P
- Subjects
- Humans, Aorta, Arteriosclerosis
- Published
- 1949
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