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1. A novel array of real-time RT-PCR assays for the rapid pathotyping of type I avian paramyxovirus (APMV-1)

Author

Fortin, Andrea, Laconi, Andrea, Monne, Isabella, Zohari, Siamak, Andersson, Kristofer, Grund, Christian, Cecchinato, Mattia, Crimaudo, Marika, Valastro, Viviana, D’Amico, Valeria, Bortolami, Alessio, Gastaldelli, Michele, Varotto, Maria, Abdelrahman, Amgad, Amarin, Nadim, Abubakar, Mustapha Bala, Belayneh, Redeat, Cyprien, Yapi Bokpè, Christodoulou, Vasiliki, Chvala, Ilya, Dodovski, Aleksandar, Ghafouri, Seyed Ali, Giasuddin, Mohammed, Hassan, Magdy, Kammon, Abdulwahab, Shittu, Ismaila, Snoeck, Chantal J., Steensel, Mieke, Suarez, David, Torchetti, Mia Kim, Tshipambe, Serge Mpiana, Ouermi Zerbo, Lamouni Habibata, Terregino, Calogero, and Panzarin, Valentina

Published
2023
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3. Vaccination of poultry against highly pathogenic avian influenza – Part 2. Surveillance and mitigation measures

Author

EFSA Panel on Animal Health and Animal Welfare, European Union Reference Laboratory for Avian Influenza, Nielsen, Søren Saxmose, Alvarez, Julio, Bicout, Dominique Joseph, Calistri, Paolo, Canali, Elisabetta, Drewe, Julian Ashley, Garin-Bastuji, Bruno, Gortázar, Christian, Herskin, Mette S., Michel, Virginie, Miranda Chueca, Miguel Ángel, Padalino, Barbara, Roberts, Helen Clare, Spoolder, Hans, Stahl, Karl, Velarde, Antonio, Viltrop, Arvo, Winckler, Christoph, Bortolami, Alessio, Guinat, Claire, Harder, Timm, Stegeman, Arjan, Terregino, Calogero, Lanfranchi, Barbara, Preite, Ludovica, Aznar, Inma, Broglia, Alessandro, Baldinelli, Francesca, Gonzales Rojas, Jose Luis, EFSA Panel on Animal Health and Animal Welfare, European Union Reference Laboratory for Avian Influenza, Nielsen, Søren Saxmose, Alvarez, Julio, Bicout, Dominique Joseph, Calistri, Paolo, Canali, Elisabetta, Drewe, Julian Ashley, Garin-Bastuji, Bruno, Gortázar, Christian, Herskin, Mette S., Michel, Virginie, Miranda Chueca, Miguel Ángel, Padalino, Barbara, Roberts, Helen Clare, Spoolder, Hans, Stahl, Karl, Velarde, Antonio, Viltrop, Arvo, Winckler, Christoph, Bortolami, Alessio, Guinat, Claire, Harder, Timm, Stegeman, Arjan, Terregino, Calogero, Lanfranchi, Barbara, Preite, Ludovica, Aznar, Inma, Broglia, Alessandro, Baldinelli, Francesca, and Gonzales Rojas, Jose Luis

Abstract

Selecting appropriate diagnostic methods that take account of the type of vaccine used is important when implementing a vaccination programme against highly pathogenic avian influenza (HPAI). If vaccination is effective, a decreased viral load is expected in the samples used for diagnosis, making molecular methods with high sensitivity the best choice. Although serological methods can be reasonably sensitive, they may produce results that are difficult to interpret. In addition to routine molecular monitoring, it is recommended to conduct viral isolation, genetic sequencing and phenotypic characterisation of any HPAI virus detected in vaccinated flocks to detect escape mutants early. Following emergency vaccination, various surveillance options based on virological testing of dead birds (?bucket sampling?) at defined intervals were assessed to be effective for early detection of HPAIV and prove disease freedom in vaccinated populations. For ducks, virological or serological testing of live birds was assessed as an effective strategy. This surveillance could be also applied in the peri-vaccination zone on vaccinated establishments, while maintaining passive surveillance in unvaccinated chicken layers and turkeys, and weekly bucket sampling in unvaccinated ducks. To demonstrate disease freedom with >?99% confidence and to detect HPAI virus sufficiently early following preventive vaccination, monthly virological testing of all dead birds up to 15 per flock, coupled with passive surveillance in both vaccinated and unvaccinated flocks, is recommended. Reducing the sampling intervals increases the sensitivity of early detection up to 100%. To enable the safe movement of vaccinated poultry during emergency vaccination, laboratory examinations in the 72?h prior to the movement can be considered as a risk mitigation measure, in addition to clinical inspection; sampling results from existing surveillance activities carried out in these 72?h could be used. In this Opinion

Published
2024

6. H7N6 highly pathogenic avian influenza in Mozambique, 2023

Author

Monjane, Iolanda Vieira Anahory, primary, Djedje, Hernâni, additional, Tamele, Esmeralda, additional, Nhabomba, Virgínia, additional, Tivane, Almiro Rogério, additional, Massicame, Zacarias Elias, additional, Arone, Dercília Mudanisse, additional, Pastori, Ambra, additional, Bortolami, Alessio, additional, Monne, Isabella, additional, Woma, Timothy, additional, Lamien, Charles E., additional, and Dundon, William G., additional

Published
2024
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8. H7N6 highly pathogenic avian influenza in Mozambique, 2023.

Author

Anahory Monjane, Iolanda Vieira, Djedje, Hernâni, Tamele, Esmeralda, Nhabomba, Virgínia, Tivane, Almiro Rogério, Massicame, Zacarias Elias, Mudanisse Arone, Dercília, Pastori, Ambra, Bortolami, Alessio, Monne, Isabella, Woma, Timothy, Lamien, Charles E., and Dundon, William G.

Published
2024
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9. Vaccination of poultry against highly pathogenic avian influenza – Part 2. Surveillance and mitigation measures.

Author

Nielsen, Søren Saxmose, Alvarez, Julio, Bicout, Dominique Joseph, Calistri, Paolo, Canali, Elisabetta, Drewe, Julian Ashley, Garin‐Bastuji, Bruno, Gortázar, Christian, Herskin, Mette S., Michel, Virginie, Miranda Chueca, Miguel Ángel, Padalino, Barbara, Roberts, Helen Clare, Spoolder, Hans, Stahl, Karl, Velarde, Antonio, Viltrop, Arvo, Winckler, Christoph, Bortolami, Alessio, and Guinat, Claire

Subjects
AVIAN influenza, VACCINATION, HAZARD mitigation, POULTRY, SERODIAGNOSIS, VACCINATION status, POULTRY products
Abstract

Selecting appropriate diagnostic methods that take account of the type of vaccine used is important when implementing a vaccination programme against highly pathogenic avian influenza (HPAI). If vaccination is effective, a decreased viral load is expected in the samples used for diagnosis, making molecular methods with high sensitivity the best choice. Although serological methods can be reasonably sensitive, they may produce results that are difficult to interpret. In addition to routine molecular monitoring, it is recommended to conduct viral isolation, genetic sequencing and phenotypic characterisation of any HPAI virus detected in vaccinated flocks to detect escape mutants early. Following emergency vaccination, various surveillance options based on virological testing of dead birds ('bucket sampling') at defined intervals were assessed to be effective for early detection of HPAIV and prove disease freedom in vaccinated populations. For ducks, virological or serological testing of live birds was assessed as an effective strategy. This surveillance could be also applied in the peri‐vaccination zone on vaccinated establishments, while maintaining passive surveillance in unvaccinated chicken layers and turkeys, and weekly bucket sampling in unvaccinated ducks. To demonstrate disease freedom with > 99% confidence and to detect HPAI virus sufficiently early following preventive vaccination, monthly virological testing of all dead birds up to 15 per flock, coupled with passive surveillance in both vaccinated and unvaccinated flocks, is recommended. Reducing the sampling intervals increases the sensitivity of early detection up to 100%. To enable the safe movement of vaccinated poultry during emergency vaccination, laboratory examinations in the 72 h prior to the movement can be considered as a risk mitigation measure, in addition to clinical inspection; sampling results from existing surveillance activities carried out in these 72 h could be used. In this Opinion, several schemes are recommended to enable the safe movement of vaccinated poultry following preventive vaccination. [ABSTRACT FROM AUTHOR]

Published
2024
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10. SARS-CoV-2 in Animal Companions: A Serosurvey in Three Regions of Southern Italy

Author

Bianco, Angelica, primary, Bortolami, Alessio, additional, Miccolupo, Angela, additional, Sottili, Roldano, additional, Ghergo, Paola, additional, Castellana, Stefano, additional, Del Sambro, Laura, additional, Capozzi, Loredana, additional, Pagliari, Matteo, additional, Bonfante, Francesco, additional, Ridolfi, Donato, additional, Bulzacchelli, Carmela, additional, Giannico, Anna, additional, and Parisi, Antonio, additional

Published
2023
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11. SARS-CoV-2 infection and replication in human gastric organoids

Author

Giobbe, Giovanni Giuseppe, Bonfante, Francesco, Jones, Brendan C., Gagliano, Onelia, Luni, Camilla, Zambaiti, Elisa, Perin, Silvia, Laterza, Cecilia, Busslinger, Georg, Stuart, Hannah, Pagliari, Matteo, Bortolami, Alessio, Mazzetto, Eva, Manfredi, Anna, Colantuono, Chiara, Di Filippo, Lucio, Pellegata, Alessandro Filippo, Panzarin, Valentina, Thapar, Nikhil, Li, Vivian Sze Wing, Eaton, Simon, Cacchiarelli, Davide, Clevers, Hans, Elvassore, Nicola, and De Coppi, Paolo

Published
2021
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12. Asymptomatic infection with clade 2.3.4.4b highly pathogenic avian influenza A(H5N1) in carnivore pets, Italy, April 2023

Author

Moreno, Ana, primary, Bonfante, Francesco, additional, Bortolami, Alessio, additional, Cassaniti, Irene, additional, Caruana, Anna, additional, Cottini, Vincenzo, additional, Cereda, Danilo, additional, Farioli, Marco, additional, Fusaro, Alice, additional, Lavazza, Antonio, additional, Lecchini, Pierdavide, additional, Lelli, Davide, additional, Maroni Ponti, Andrea, additional, Nassuato, Claudia, additional, Pastori, Ambra, additional, Rovida, Francesca, additional, Ruocco, Luigi, additional, Sordilli, Marco, additional, Baldanti, Fausto, additional, and Terregino, Calogero, additional

Published
2023
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14. Vaccination of poultry against highly pathogenic avian influenza – part 1. Available vaccines and vaccination strategies

Author

Nielsen, Søren Saxmose, Alvarez, Julio, Bicout, Dominique Joseph, Calistri, Paolo, Canali, Elisabetta, Drewe, Julian Ashley, Garin-Bastuji, Bruno, Gonzales Rojas, Jose Luis, Gortázar, Christian, Herskin, Mette, Michel, Virginie, Miranda Chueca, Miguel Ángel, Padalino, Barbara, Roberts, Helen Clare, Spoolder, Hans, Stahl, Karl, Velarde, Antonio, Winckler, Christoph, Bastino, Eleonora, Bortolami, Alessio, Guinat, Claire, Harder, Timm, Stegeman, Arjan, Terregino, Calogero, Aznar Asensio, Inmaculada, Mur, Lina, Broglia, Alessandro, Baldinelli, Francesca, Viltrop, Arvo, Nielsen, Søren Saxmose, Alvarez, Julio, Bicout, Dominique Joseph, Calistri, Paolo, Canali, Elisabetta, Drewe, Julian Ashley, Garin-Bastuji, Bruno, Gonzales Rojas, Jose Luis, Gortázar, Christian, Herskin, Mette, Michel, Virginie, Miranda Chueca, Miguel Ángel, Padalino, Barbara, Roberts, Helen Clare, Spoolder, Hans, Stahl, Karl, Velarde, Antonio, Winckler, Christoph, Bastino, Eleonora, Bortolami, Alessio, Guinat, Claire, Harder, Timm, Stegeman, Arjan, Terregino, Calogero, Aznar Asensio, Inmaculada, Mur, Lina, Broglia, Alessandro, Baldinelli, Francesca, and Viltrop, Arvo

Abstract

Several vaccines have been developed against highly pathogenic avian influenza (HPAI), mostly inactivated whole-virus vaccines for chickens. In the EU, one vaccine is authorised in chickens but is not fully efficacious to stop transmission, highlighting the need for vaccines tailored to diverse poultry species and production types. Off-label use of vaccines is possible, but effectiveness varies. Vaccines are usually injectable, a time-consuming process. Mass-application vaccines outside hatcheries remain rare. First vaccination varies from in-ovo to 6 weeks of age. Data about immunity onset and duration in the target species are often unavailable, despite being key for effective planning. Minimising antigenic distance between vaccines and field strains is essential, requiring rapid updates of vaccines to match circulating strains. Generating harmonised vaccine efficacy data showing vaccine ability to reduce transmission is crucial and this ability should be also assessed in field trials. Planning vaccination requires selecting the most adequate vaccine type and vaccination scheme. Emergency protective vaccination is limited to vaccines that are not restricted by species, age or pre-existing vector-immunity, while preventive vaccination should prioritise achieving the highest protection, especially for the most susceptible species in high-risk transmission areas. Model simulations in France, Italy and The Netherlands revealed that (i) duck and turkey farms are more infectious than chickens, (ii) depopulating infected farms only showed limitations in controlling disease spread, while 1-km ring-culling performed better than or similar to emergency preventive ring-vaccination scenarios, although with the highest number of depopulated farms, (iii) preventive vaccination of the most susceptible species in high-risk transmission areas was the best option to minimise the outbreaks' number and duration, (iv) during outbreaks in such areas, emergency protective vaccination

Published
2023

15. The Luciferase Immunoprecipitation System (LIPS) Targeting the Spike Protein of SARS-CoV-2 Is More Accurate than Nucleoprotein-Based LIPS and ELISAs for Mink Serology

Author

Auer, Agathe, primary, Bortolami, Alessio, additional, Berguido, Francisco J., additional, Bonfante, Francesco, additional, Terregino, Calogero, additional, Natale, Alda, additional, Fincato, Alice, additional, Colitti, Barbara, additional, Rosati, Sergio, additional, Lamien, Charles E., additional, and Cattoli, Giovanni, additional

Published
2023
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16. Host Response of Syrian Hamster to SARS-CoV-2 Infection including Differences with Humans and between Sexes

Author

Castellan, Martina, primary, Zamperin, Gianpiero, additional, Franzoni, Giulia, additional, Foiani, Greta, additional, Zorzan, Maira, additional, Drzewnioková, Petra, additional, Mancin, Marzia, additional, Brian, Irene, additional, Bortolami, Alessio, additional, Pagliari, Matteo, additional, Oggiano, Annalisa, additional, Vascellari, Marta, additional, Panzarin, Valentina, additional, Crovella, Sergio, additional, Monne, Isabella, additional, Terregino, Calogero, additional, De Benedictis, Paola, additional, and Leopardi, Stefania, additional

Published
2023
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17. One-Year Surveillance of SARS-CoV-2 Exposure in Stray Cats and Kennel Dogs from Northeastern Italy

Author

Bellinati, Laura, primary, Campalto, Mery, additional, Mazzotta, Elisa, additional, Ceglie, Letizia, additional, Cavicchio, Lara, additional, Mion, Monica, additional, Lucchese, Laura, additional, Salomoni, Angela, additional, Bortolami, Alessio, additional, Quaranta, Erika, additional, Magarotto, Jacopo, additional, Favarato, Mosè, additional, Squarzon, Laura, additional, and Natale, Alda, additional

Published
2022
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18. Host response of Syrian hamster to SARS-CoV-2 infection, including differences with humans and between sexes

Author

Castellan, Martina, primary, Zamperin, Gianpiero, additional, Franzoni, Giulia, additional, Foiani, Greta, additional, Zorzan, Maira, additional, Drzewnioková, Petra, additional, Mancin, Marzia, additional, Brian, Irene, additional, Bortolami, Alessio, additional, Pagliari, Matteo, additional, Oggiano, Annalisa, additional, Vascellari, Marta, additional, Panzarin, Valentina, additional, Crovella, Sergio, additional, Monne, Isabella, additional, Terregino, Calogero, additional, De Benedictis, Paola, additional, and Leopardi, Stefania, additional

Published
2022
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19. Rapid spread of a new West Nile virus lineage 1 associated with increased risk of neuroinvasive disease during a large outbreak in Italy in 2022

Author

Barzon, Luisa, primary, Pacenti, Monia, additional, Montarsi, Fabrizio, additional, Fornasiero, Diletta, additional, Gobbo, Federica, additional, Quaranta, Erika, additional, Monne, Isabella, additional, Fusaro, Alice, additional, Volpe, Andrea, additional, Sinigaglia, Alessandro, additional, Riccetti, Silvia, additional, Dal Molin, Emanuela, additional, Satto, Sorsha, additional, Lisi, Vittoria, additional, Gobbi, Federico, additional, Galante, Silvia, additional, Feltrin, Giuseppe, additional, Valeriano, Valerio, additional, Favero, Laura, additional, Russo, Francesca, additional, Mazzucato, Matteo, additional, Bortolami, Alessio, additional, Mulatti, Paolo, additional, Terregino, Calogero, additional, and Capelli, Gioia, additional

Published
2022
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20. Rapid spread of a new West Nile virus lineage 1 associated with increased risk of neuroinvasive disease during a large outbreak in northern Italy, 2022: One Health analysis

Author

Barzon, Luisa, Pacenti, Monia, Montarsi, Fabrizio, Fornasiero, Diletta, Gobbo, Federica, Quaranta, Erika, Monne, Isabella, Fusaro, Alice, Volpe, Andrea, Sinigaglia, Alessandro, Riccetti, Silvia, Molin, Emanuela Dal, Satto, Sorsha, Lisi, Vittoria, Gobbi, Federico, Galante, Silvia, Feltrin, Giuseppe, Valeriano, Valerio, Favero, Laura, Russo, Francesca, Mazzucato, Matteo, Bortolami, Alessio, Mulatti, Paolo, Terregino, Calogero, and Capelli, Gioia

Subjects
West Nile virus, bird, fever, lineage, mosquito, neuroinvasive disease
Abstract

A new strain of WNV lineage 1 (WNV - 1) emerged in the Veneto Region, northern Italy, in 2021, eight years after the last outbreak of WNV - 1 in Italy. The virus, which co-circulates with WNV-2, has become endemic in the Region, where, in 2022, most human cases of neuroinvasive disease (WNND) reported in Europe have occurred.Comparative analysis of the epidemiology and clinical presentation of WNV-1 and WNV-2 infection in humans, as well as the temporal and geographic distribution of WNV-1 and WNV-2 among wild birds and Culex pipiens mosquitoes in Veneto, from May 16th to August 21st, 2022, to determine if the high number of WNND cases was associated with WNV-1.As of August 21st, 2022, 222 human cases of WNV infection were confirmed by molecular testing, including 103 with fever (WNF) and 119 with WNND. WNV lineage was determined in 201 (90.5%) cases, comprising 138 WNV-1 and 63 WNV-2 infections. During the same period, 35 blood donors tested positive, including 30 in whom WNV lineage was determined (13 WNV-1 and 17 WNV-2). Comparative analysis of the distribution of WNV-1 and WNV-2 infections among WNND cases, WNF cases and WNV-positive blood donors showed that patients with WNND were more likely to have WNV-1 infection than blood donors (odds ratio 3.44; 95% CI 95% 1.54 to 8.24; p = 0.0043). As observed in humans, in wild birds WNV-1 had higher infectious rate (IR) and showed a more rapid expansion than WNV-2. At variance, the distribution of the two lineages was more even in mosquitoes, but with a trend of rapid increase of WNV-1 IR over WNV-2.Comparative analysis of WNV-1 vs WNV-2 infection in humans, wild birds, and mosquitos showed a rapid expansion of WNV-1 and suggested that WNV-1 infected patients might have an increased risk to develop severe disease.

Published
2022

21. Emergence of a Reassortant 2.3.4.4b Highly Pathogenic H5N1 Avian Influenza Virus Containing H9N2 PA Gene in Burkina Faso, West Africa, in 2021

Author

Ouoba, Lalidia Bruno, primary, Habibata-Zerbo, Lamouni, additional, Zecchin, Bianca, additional, Barbierato, Giacomo, additional, Hamidou-Ouandaogo, Sandaogo, additional, Palumbo, Elisa, additional, Giussani, Edoardo, additional, Bortolami, Alessio, additional, Niang, Mamadou, additional, Traore-Kam, Adele, additional, Terregino, Calogero, additional, Guitti-Kindo, Mariétou, additional, Angot, Angelique, additional, Guigma, Dominique, additional, Barro, Nicolas, additional, Fusaro, Alice, additional, and Monne, Isabella, additional

Published
2022
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22. Protective Efficacy of H9N2 Avian Influenza Vaccines Inactivated by Ionizing Radiation Methods Administered by the Parenteral or Mucosal Routes

Author

Bortolami, Alessio, primary, Mazzetto, Eva, additional, Kangethe, Richard Thiga, additional, Wijewardana, Viskam, additional, Barbato, Mario, additional, Porfiri, Luca, additional, Maniero, Silvia, additional, Mazzacan, Elisa, additional, Budai, Jane, additional, Marciano, Sabrina, additional, Panzarin, Valentina, additional, Terregino, Calogero, additional, Bonfante, Francesco, additional, and Cattoli, Giovanni, additional

Published
2022
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24. Protective Efficacy of H9N2 Avian Influenza Vaccines Inactivated by Ionizing Radiation Methods Administered by the Parenteral or Mucosal Routes

Author

Bortolami, Alessio, Mazzetto, Eva, Kangethe, Richard Thiga, Wijewardana, Viskam, Barbato, Mario, Porfiri, Luca, Maniero, Silvia, Mazzacan, Elisa, Budai, Jane, Marciano, Sabrina, Panzarin, Valentina, Terregino, Calogero, Bonfante, Francesco, Cattoli, Giovanni, Barbato, Mario (ORCID:0000-0002-7203-1549), Bortolami, Alessio, Mazzetto, Eva, Kangethe, Richard Thiga, Wijewardana, Viskam, Barbato, Mario, Porfiri, Luca, Maniero, Silvia, Mazzacan, Elisa, Budai, Jane, Marciano, Sabrina, Panzarin, Valentina, Terregino, Calogero, Bonfante, Francesco, Cattoli, Giovanni, and Barbato, Mario (ORCID:0000-0002-7203-1549)

Abstract

H9N2 viruses have become, over the last 20 years, one of the most diffused poultry pathogens and have reached a level of endemicity in several countries. Attempts to control the spread and reduce the circulation of H9N2 have relied mainly on vaccination in endemic countries. However, the high level of adaptation to poultry, testified by low minimum infectious doses, replication to high titers, and high transmissibility, has severely hampered the results of vaccination campaigns. Commercially available vaccines have demonstrated high efficacy in protecting against clinical disease, but variable results have also been observed in reducing the level of replication and viral shedding in domestic poultry species. Antigenic drift and increased chances of zoonotic infections are the results of incomplete protection offered by the currently available vaccines, of which the vast majority are based on formalin-inactivated whole virus antigens. In our work, we evaluated experimental vaccines based on an H9N2 virus, inactivated by irradiation treatment, in reducing viral shedding upon different challenge doses and compared their efficacy with formalin-inactivated vaccines. Moreover, we evaluated mucosal delivery of inactivated antigens as an alternative route to subcutaneous and intramuscular vaccination. The results showed complete protection and prevention of replication in subcutaneously vaccinated Specific Pathogen Free White Leghorn chickens at low-to-intermediate challenge doses but a limited reduction of shedding at a high challenge dose. Mucosally vaccinated chickens showed a more variable response to experimental infection at all tested challenge doses and the main effect of vaccination attained the reduction of infected birds in the early phase of infection. Concerning mucosal vaccination, the irradiated vaccine was the only one affording complete protection from infection at the lowest challenge dose. Vaccine formulations based on H9N2 inactivated by irradiation demo

Published
2022

25. Molecular Monitoring of SARS-CoV-2 in Different Sewage Plants in Venice and the Implications for Genetic Surveillance

Author

Brian, Irene, primary, Manuzzi, Alice, additional, Dalla Rovere, Giulia, additional, Giussani, Edoardo, additional, Palumbo, Elisa, additional, Fusaro, Alice, additional, Bonfante, Francesco, additional, Bortolami, Alessio, additional, Quaranta, Erika Giorgia, additional, Monne, Isabella, additional, Patarnello, Tomaso, additional, Bargelloni, Luca, additional, Terregino, Calogero, additional, Holmes, Edward C., additional, Todesco, Giovanna, additional, Sorrentino, Francesco, additional, Berton, Andrea, additional, Badetti, Christian, additional, Carrer, Claudio, additional, Ferrari, Giorgio, additional, Zincone, Cinzia, additional, Milan, Massimo, additional, and Panzarin, Valentina, additional

Published
2022
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26. One-Year Surveillance of SARS-CoV-2 Exposure in Stray Cats and Kennel Dogs from Northeastern Italy.

Author

Bellinati, Laura, Campalto, Mery, Mazzotta, Elisa, Ceglie, Letizia, Cavicchio, Lara, Mion, Monica, Lucchese, Laura, Salomoni, Angela, Bortolami, Alessio, Quaranta, Erika, Magarotto, Jacopo, Favarato, Mosè, Squarzon, Laura, and Natale, Alda

Subjects
FERAL dogs, CATS, SARS-CoV-2, FERAL cats, DOGS, VETERINARY public health, REVERSE transcriptase
Abstract

Dogs and cats are susceptible to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). During the pandemic, several studies have been performed on owned cats and dogs, whereas limited data are available on the exposure to stray animals. The objective of this study was to investigate the exposure to SARS-CoV-2 of feral cats and kennel dogs in northeastern Italy, through serological and molecular methods. From May 2021 to September 2022, public health veterinary services collected serum, oropharyngeal, and rectal swab samples from 257 free-roaming dogs newly introduced to shelters, and from 389 feral cats examined during the routinely trap–neutered–return programs. The swabs were analyzed for viral RNA through a real-time reverse transcriptase PCR (rRT-PCR), and sera were tested for the presence of the specific antibody against SARS-CoV-2 (enzyme-linked immunosorbent assay). Serology was positive in nine dogs (9/257) and three cats (3/389), while two asymptomatic cats tested positive to rRT-PCR. One cat turned out to be positive both for serology and molecular analysis. In addition, this study described the case of a possible human-to-animal SARS-CoV-2 transmission in a cat that travelled in close contact to a COVID-19-positive refugee from Ukraine. This study shows that SARS-CoV-2 can infect, in natural conditions, stray cats and kennel dogs in northeastern Italy, although with a low prevalence. [ABSTRACT FROM AUTHOR]

Published
2023
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27. Omicron Neutralizing and Anti-SARS-CoV-2 S-RBD Antibodies in Naïve and Convalescent Populations After Homologous and Heterologous Boosting With an mRNA Vaccine

Author

Pagliari, Matteo, primary, Mazzetto, Eva, additional, Gastaldelli, Michele, additional, Bortolami, Alessio, additional, Donà, Daniele, additional, Padoan, Andrea, additional, Di Chiara, Costanza, additional, Pezzani, Maria Diletta, additional, Cosma, Chiara, additional, Napolitan, Alessandra, additional, Quaranta, Erika Giorgia, additional, Giussani, Edoardo, additional, Fusaro, Alice, additional, Pascarella, Michela, additional, Aita, Ada, additional, Liberati, Cecilia, additional, Lorusso, Alessio, additional, Monne, Isabella, additional, De Rossi, Anita, additional, Basso, Daniela, additional, Porru, Stefano, additional, Ricci, Antonia, additional, Terregino, Calogero, additional, Plebani, Mario, additional, Tacconelli, Evelina, additional, Giaquinto, Carlo, additional, and Bonfante, Francesco, additional

Published
2022
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28. Neutralizing antibody titers six months after Comirnaty vaccination: kinetics and comparison with SARS-CoV-2 immunoassays

Author

Padoan, Andrea, primary, Cosma, Chiara, additional, Bonfante, Francesco, additional, della Rocca, Foscarina, additional, Barbaro, Francesco, additional, Santarossa, Claudia, additional, Dall’Olmo, Luigi, additional, Pagliari, Matteo, additional, Bortolami, Alessio, additional, Cattelan, Annamaria, additional, Cianci, Vito, additional, Basso, Daniela, additional, and Plebani, Mario, additional

Published
2021
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29. Development of a Novel Assay Based on Plant-Produced Infectious Bursal Disease Virus VP3 for the Differentiation of Infected From Vaccinated Animals

Author

Bortolami, Alessio, primary, Donini, Marcello, additional, Marusic, Carla, additional, Lico, Chiara, additional, Drissi Touzani, Charifa, additional, Gobbo, Federica, additional, Mazzacan, Elisa, additional, Fortin, Andrea, additional, Panzarin, Valentina Maria, additional, Bonfante, Francesco, additional, Baschieri, Selene, additional, and Terregino, Calogero, additional

Published
2021
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30. Heterogeneity of Early Host Response to Infection with Four Low-Pathogenic H7 Viruses with a Different Evolutionary History in the Field

Author

Zamperin, Gianpiero, primary, Bianco, Alice, additional, Smith, Jacqueline, additional, Bortolami, Alessio, additional, Vervelde, Lonneke, additional, Schivo, Alessia, additional, Fortin, Andrea, additional, Marciano, Sabrina, additional, Panzarin, Valentina, additional, Mazzetto, Eva, additional, Milani, Adelaide, additional, Berhane, Yohannes, additional, Digard, Paul, additional, Bonfante, Francesco, additional, and Monne, Isabella, additional

Published
2021
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31. Asymptomatic and Mild SARS-CoV-2 Infections Elicit Lower Immune Activation and Higher Specific Neutralizing Antibodies in Children Than in Adults

Author

Petrara, Maria Raffaella, primary, Bonfante, Francesco, additional, Costenaro, Paola, additional, Cantarutti, Anna, additional, Carmona, Francesco, additional, Ruffoni, Elena, additional, Di Chiara, Costanza, additional, Zanchetta, Marisa, additional, Barzon, Luisa, additional, Donà, Daniele, additional, Da Dalt, Liviana, additional, Bortolami, Alessio, additional, Pagliari, Matteo, additional, Plebani, Mario, additional, Rossi, Paolo, additional, Cotugno, Nicola, additional, Palma, Paolo, additional, Giaquinto, Carlo, additional, and De Rossi, Anita, additional

Published
2021
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32. Mild SARS-CoV-2 Infections and Neutralizing Antibody Titers

Author

Bonfante, Francesco, primary, Costenaro, Paola, additional, Cantarutti, Anna, additional, Di Chiara, Costanza, additional, Bortolami, Alessio, additional, Petrara, Maria Raffaella, additional, Carmona, Francesco, additional, Pagliari, Matteo, additional, Cosma, Chiara, additional, Cozzani, Sandra, additional, Mazzetto, Eva, additional, Di Salvo, Giovanni, additional, Da Dalt, Liviana, additional, Palma, Paolo, additional, Barzon, Luisa, additional, Corrao, Giovanni, additional, Terregino, Calogero, additional, Padoan, Andrea, additional, Plebani, Mario, additional, De Rossi, Anita, additional, Donà, Daniele, additional, and Giaquinto, Carlo, additional

Published
2021
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33. Serological Detection of SARS-CoV-2 Antibodies in Naturally-Infected Mink and Other Experimentally-Infected Animals

Author

Berguido, Francisco J., primary, Burbelo, Peter D., additional, Bortolami, Alessio, additional, Bonfante, Francesco, additional, Wernike, Kerstin, additional, Hoffmann, Donata, additional, Balkema-Buschmann, Anne, additional, Beer, Martin, additional, Dundon, William G., additional, Lamien, Charles E., additional, and Cattoli, Giovanni, additional

Published
2021
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35. SARS-CoV-2 infection and replication in human gastric organoids

Author

Hubrecht Institute with UMC, Giobbe, Giovanni Giuseppe, Bonfante, Francesco, Jones, Brendan C., Gagliano, Onelia, Luni, Camilla, Zambaiti, Elisa, Perin, Silvia, Laterza, Cecilia, Busslinger, Georg, Stuart, Hannah, Pagliari, Matteo, Bortolami, Alessio, Mazzetto, Eva, Manfredi, Anna, Colantuono, Chiara, Di Filippo, Lucio, Pellegata, Alessandro Filippo, Panzarin, Valentina, Thapar, Nikhil, Li, Vivian Sze Wing, Eaton, Simon, Cacchiarelli, Davide, Clevers, Hans, Elvassore, Nicola, De Coppi, Paolo, Hubrecht Institute with UMC, Giobbe, Giovanni Giuseppe, Bonfante, Francesco, Jones, Brendan C., Gagliano, Onelia, Luni, Camilla, Zambaiti, Elisa, Perin, Silvia, Laterza, Cecilia, Busslinger, Georg, Stuart, Hannah, Pagliari, Matteo, Bortolami, Alessio, Mazzetto, Eva, Manfredi, Anna, Colantuono, Chiara, Di Filippo, Lucio, Pellegata, Alessandro Filippo, Panzarin, Valentina, Thapar, Nikhil, Li, Vivian Sze Wing, Eaton, Simon, Cacchiarelli, Davide, Clevers, Hans, Elvassore, Nicola, and De Coppi, Paolo

Published
2021

36. SARS-Cov-2 Natural Infection in a Symptomatic Cat: Diagnostic, Clinical and Medical Management in a One Health Vision

Author

Natale, Alda, primary, Mazzotta, Elisa, additional, Mason, Nicoletta, additional, Ceglie, Letizia, additional, Mion, Monica, additional, Stefani, Annalisa, additional, Fincato, Alice, additional, Bonfante, Francesco, additional, Bortolami, Alessio, additional, Monne, Isabella, additional, Bellinati, Laura, additional, Guadagno, Carmine, additional, Quaranta, Erika, additional, Pastori, Ambra, additional, and Terregino, Calogero, additional

Published
2021
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37. Analytical and clinical performances of a SARS-CoV-2 S-RBD IgG assay: comparison with neutralization titers

Author

Padoan, Andrea, primary, Bonfante, Francesco, additional, Cosma, Chiara, additional, Di Chiara, Costanza, additional, Sciacovelli, Laura, additional, Pagliari, Matteo, additional, Bortolami, Alessio, additional, Costenaro, Paola, additional, Musso, Giulia, additional, Basso, Daniela, additional, Giaquinto, Carlo, additional, and Plebani, Mario, additional

Published
2021
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38. Virological and immunological features of SARS-CoV-2-infected children who develop neutralizing antibodies

Author

Cotugno, Nicola, primary, Ruggiero, Alessandra, additional, Bonfante, Francesco, additional, Petrara, Maria Raffaella, additional, Zicari, Sonia, additional, Pascucci, Giuseppe Rubens, additional, Zangari, Paola, additional, De Ioris, Maria Antonietta, additional, Santilli, Veronica, additional, Manno, E.C., additional, Amodio, Donato, additional, Bortolami, Alessio, additional, Pagliari, Matteo, additional, Concato, Carlo, additional, Linardos, Giulia, additional, Campana, Andrea, additional, Donà, Daniele, additional, Giaquinto, Carlo, additional, Brodin, Petter, additional, Rossi, Paolo, additional, De Rossi, Anita, additional, Palma, Paolo, additional, Bernardi, Stefania, additional, Romani, Lorenza, additional, Pansa, Paola, additional, Chiurchiú, Sara, additional, Finocchi, Andrea, additional, Cancrini, Caterina, additional, Lancella, Laura, additional, Cursi, Laura, additional, De Luca, Maia, additional, Cutrera, Renato, additional, Sessa, Libera, additional, Morrocchi, Elena, additional, Medri, Chiara, additional, Putignani, Lorenza, additional, Calò Carducci, F.I., additional, D’Argenio, Patrizia, additional, Ciofi degli Atti, Marta, additional, D’Amore, Carmen, additional, Piccioni, Livia, additional, Di Giuseppe, Martina, additional, Jenkner, Alessandro, additional, Giancotta, Carmela, additional, and Krzysztofiak, Andrzej, additional

Published
2021
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39. The expression in plants of an engineered VP2 protein of Infectious Bursal Disease Virus induces formation of structurally heterogeneous particles that protect from a very virulent viral strain

Author

Marusic, Carla, primary, Drissi Touzani, Charifa, additional, Bortolami, Alessio, additional, Donini, Marcello, additional, Zanardello, Claudia, additional, Lico, Chiara, additional, Rage, Emile, additional, Fellahi, Siham, additional, El Houadfi, Mohammed, additional, Terregino, Calogero, additional, and Baschieri, Selene, additional

Published
2021
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41. Neutralizing antibody titers six months after Comirnaty vaccination: kinetics and comparison with SARS-CoV-2 immunoassays.

Author

Padoan, Andrea, Cosma, Chiara, Bonfante, Francesco, della Rocca, Foscarina, Barbaro, Francesco, Santarossa, Claudia, Dall'Olmo, Luigi, Pagliari, Matteo, Bortolami, Alessio, Cattelan, Annamaria, Cianci, Vito, Basso, Daniela, and Plebani, Mario

Subjects
COVID-19, ANTIBODY titer, IMMUNOASSAY, SARS-CoV-2, MEDICAL personnel, VACCINATION
Abstract

Keywords: binding antibodies; BNT162b2; Comirnaty; COVID-19; immunoassays; immunological response; kinetics; neutralizing antibodies; SARS-CoV-2 vaccine; serology EN binding antibodies BNT162b2 Comirnaty COVID-19 immunoassays immunological response kinetics neutralizing antibodies SARS-CoV-2 vaccine serology 456 463 8 02/07/22 20220201 NES 220201 Introduction The coronavirus disease 2019 (COVID-19) pandemic caused by SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) continues to sustain a global public health crisis, despite the availability of vaccines that have dramatically reduced severe disease, hospitalization and mortality [[1]]. However, the decrease in serum antibodies against SARS-CoV-2 have raised questions concerning long-term immunity, since decreased antibody levels have been associated with breakthrough infections, prompting the consideration of additional vaccine booster doses [[4]]. The waning of serum antibodies and neutralizing antibodies against SARS-CoV-2 has been widely documented [[16]], although several studies have underlined that levels of vaccine-induced Ab persist also 6 months after the second dose [[18]]. [Extracted from the article]

Published
2022
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42. SARS-CoV-2 infection and replication in human fetal and pediatric gastric organoids

Author

Giobbe, Giovanni Giuseppe, primary, Bonfante, Francesco, additional, Zambaiti, Elisa, additional, Gagliano, Onelia, additional, Jones, Brendan C., additional, Luni, Camilla, additional, Laterza, Cecilia, additional, Perin, Silvia, additional, Stuart, Hannah T., additional, Pagliari, Matteo, additional, Bortolami, Alessio, additional, Mazzetto, Eva, additional, Manfredi, Anna, additional, Colantuono, Chiara, additional, Di Filippo, Lucio, additional, Pellegata, Alessandro, additional, Li, Vivian Sze Wing, additional, Eaton, Simon, additional, Thapar, Nikhil, additional, Cacchiarelli, Davide, additional, Elvassore, Nicola, additional, and De Coppi, Paolo, additional

Published
2020
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43. Replication of Influenza D Viruses of Bovine and Swine Origin in Ovine Respiratory Explants and Their Attachment to the Respiratory Tract of Bovine, Sheep, Goat, Horse, and Swine

Author

Mazzetto, Eva, primary, Bortolami, Alessio, additional, Fusaro, Alice, additional, Mazzacan, Elisa, additional, Maniero, Silvia, additional, Vascellari, Marta, additional, Beato, Maria Serena, additional, Schiavon, Eliana, additional, Chiapponi, Chiara, additional, Terregino, Calogero, additional, Monne, Isabella, additional, and Bonfante, Francesco, additional

Published
2020
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44. First detection of highly pathogenic H5N6 avian influenza virus on the African continent

Author

Shittu, Ismaila, primary, Bianco, Alice, additional, Gado, Dorcas, additional, Mkpuma, Nicodemus, additional, Sulaiman, Lanre, additional, Laleye, Agnes, additional, Gobbo, Federica, additional, Bortolami, Alessio, additional, Bonfante, Francesco, additional, Vakuru, Columba, additional, Meseko, Clement, additional, Fusaro, Alice, additional, Shamaki, David, additional, Alabi, Olaniran, additional, Terregino, Calogero, additional, and Joannis, Tony, additional

Published
2020
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45. Virological and Immunological Features of SARS-CoV-2 Infected Children Developing Specific and Neutralizing Antibodies

Author

Cotugno, Nicola, primary, Ruggiero, Alessandra, additional, Bonfante, Francesco, additional, Pertrara, Maria Raffella, additional, Zicari, Sonia, additional, Pascucci, Giuseppe Rubens, additional, Zangari, Paola, additional, De Ioris, Maria Antonietta, additional, Santilli, Veronica, additional, Manno, Emma, additional, Amodio, Donato, additional, Bortolami, Alessio, additional, Pagliari, Matteo, additional, Concato, Carlo, additional, Linardos, Giulia, additional, Campana, Andrea, additional, Donà, Daniele, additional, Giaquinto, Carlo, additional, Team, CACTUS Study, additional, Brodin, Petter, additional, Rossi, Paolo, additional, De Rossi, Anita, additional, and Palma, Paolo, additional

Published
2020
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46. Environmental surveillance identifies multiple introductions of MRSA CC398 in an Equine Veterinary Hospital in the UK, 2011–2016

Author

Bortolami, Alessio, Williams, Nicola J, McGowan, Catherine M, Kelly, Padraig G, Archer, Debra C, Corro, Michela, Pinchbeck, Gina, Saunders, Christine J, and Timofte, Dorina

Subjects
Science, Medicine
Abstract

Bacterial environmental and surgical site infection (SSI) surveillance was implemented from 2011–2016 in a UK Equine Referral Veterinary Hospital and identified 81 methicillin-resistant Staphylococcus aureus (MRSA) isolates. A cluster of MRSA SSIs occurred in early 2016 with the isolates confirmed as ST398 by multilocus sequence typing (MLST), which prompted retrospective analysis of all MRSA isolates obtained from the environment (n = 62), SSIs (n = 13) and hand plates (n = 6) in the past five years. Sixty five of these isolates were typed to CC398 and a selection of these (n = 38) were further characterised for resistance and virulence genes, SCCmec and spa typing. Overall, MRSA was identified in 62/540 (11.5%) of environmental samples, 6/81 of the hand-plates (7.4%) and 13/208 of the SSIs (6.3%). spa t011 was the most frequent (24/38) and Based Upon Repeat Pattern (BURP) analysis identified spa t011 as one of the two group founders of the main spa CC identified across the five years (spa CC011/3423). However, 3 singletons (t073, t786, t064) were also identified suggesting separate introductions into the hospital environment. This long-term MRSA surveillance study revealed multiple introductions of MRSA CC398 in a UK Equine Hospital, identifying an emerging zoonotic pathogen so far only sporadically recorded in the UK.

Published
2017

47. Replication kinetics and cellular tropism of emerging reoviruses in sheep and swine respiratory ex vivo organ cultures

Author

Di Teodoro, Giovanni, primary, Bortolami, Alessio, additional, Teodori, Liana, additional, Leone, Alessandra, additional, D’Alterio, Nicola, additional, Malatesta, Daniela, additional, Rosamilia, Alfonso, additional, Colaianni, Maria Loredana, additional, Petrini, Antonio, additional, Terregino, Calogero, additional, Savini, Giovanni, additional, Bonfante, Francesco, additional, and Lorusso, Alessio, additional

Published
2019
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