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32 results on '"Bordy, Thomas"'

3. Optical calibration protocol for an x-ray and optical multimodality tomography system dedicated to small-animal examination

Author

Da Silva, Anabela, Leabad, Mehdi, Driol, Clemence, Bordy, Thomas, Debourdeau, Mathieu, Dinten, Jean-Marc, Peltie, Philippe, and Rizo, Philippe

Subjects
Animal experimentation -- Methods, Calibration -- Methods, X-rays -- Usage, Optical tomography -- Usage, Optical tomography -- Methods, Astronomy, Physics
Abstract

A small-animal multimodality tomography system dedicated to the coregistration of fluorescence optical signal and x-ray measurements has been developed in our laboratory. The purpose of such a system is to offer the possibility of getting in vivo anatomical and functional information simultaneously. Moreover, anatomical measurements can be used as a regularization factor to achieve more accurate reconstructions of the biodistribution of fluorochromes and to speed up treatment. A dedicated acquisition protocol has been established, and the methodology of the reconstruction of the three-dimensional distribution of the biomarkers under cylindrical geometry consistent with classic computed tomography has been implemented. A phantom study was conducted to evaluate and to fix the parameters for the coregistration. These test experiments were reproduced by considering anesthetized mice that had thin glass tubes containing fluorochromes inserted into their esophagus. The instrument is also used for an in vivo biological study conducted on mice with lung tumors, tagged with near-infrared optical probes (targeting probes such as Transferin-AlexaFluor750). OCIS codes: 170.3880, 110.6955, 110.4190, 170.6280, 170.7440, 170.3660.

Published
2009

5. 3D lens-free time-lapse microscopy for 3D cell culture

Author

Berdeu, Anthony, Momey, Fabien, Laperrousaz, Bastien, Bordy, Thomas, Picollet-D'hahan, Nathalie, Allier, Cédric, Laboratoire Hubert Curien / Eris, Laboratoire Hubert Curien [Saint Etienne] (LHC), Institut d'Optique Graduate School (IOGS)-Université Jean Monnet [Saint-Étienne] (UJM)-Centre National de la Recherche Scientifique (CNRS)-Institut d'Optique Graduate School (IOGS)-Université Jean Monnet [Saint-Étienne] (UJM)-Centre National de la Recherche Scientifique (CNRS), Université Jean Monnet [Saint-Étienne] (UJM)-Centre National de la Recherche Scientifique (CNRS)-Institut d'Optique Graduate School (IOGS)-Université Jean Monnet [Saint-Étienne] (UJM)-Centre National de la Recherche Scientifique (CNRS)-Institut d'Optique Graduate School (IOGS), Institut d'Optique Graduate School (IOGS)-Université Jean Monnet [Saint-Étienne] (UJM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physique de l'ENS Lyon (Phys-ENS), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Electronique et des Technologies de l'Information (CEA-LETI), Université Grenoble Alpes (UGA)-Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Laboratoire de Biologie à Grande Échelle (BGE - UMR S1038), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes (UGA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA))

Subjects
Physics::Optics, [SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing, ComputingMilieux_MISCELLANEOUS
Abstract

International audience; We present a new 3D lens-free microscope for large 3D biological samples and a dedicated reconstruction algorithm based on the Fourier diffraction theorem as used in standard diffractive tomography [1, 2]. It uses a regularised inverse problem formalism directly applied on the whole dataset and the 3D object to retrieve. To our knowledge, this is the first time that such an inverse problem approach is implemented in the context of lens-free diffractive tomography to reconstruct large volumes of unstained biological samples.

Published
2017

13. 3D lens-free time-lapse microscopy for 3D cell culture

Author

Berdeu, Anthony, additional, Momey, Fabien, additional, Laperrousaz, Bastien, additional, Bordy, Thomas, additional, Gidrol, Xavier, additional, Dinten, Jean-Marc, additional, Picollet-D'hahan, Nathalie, additional, and Allier, Cédric, additional

Published
2017
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15. Dynamics of cell and tissue growth acquired by means of 25 mm² to 10 cm² lensfree imaging

Author

Momey, Fabien, Coutard, Jean-Guillaume, Bordy, Thomas, Navarro, Fabrice, Menneteau, Mathilde, Dinten, Jean-Marc, Allier, Cédric, Laboratoire Hubert Curien [Saint Etienne] (LHC), Université Jean Monnet [Saint-Étienne] (UJM)-Centre National de la Recherche Scientifique (CNRS)-Institut d'Optique Graduate School (IOGS), Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), SPIE, Laboratoire Hubert Curien (LHC), and Institut d'Optique Graduate School (IOGS)-Université Jean Monnet - Saint-Étienne (UJM)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[INFO.INFO-IM]Computer Science [cs]/Medical Imaging
Abstract

International audience; In this paper, we discuss a new methodology based on lens-free imaging to perform wound healing assay with unprecedented statistics. Our video lens-free microscopy setup is a simple optical system featuring only a CMOS sensor and a semi coherent illumination system. Yet it is a powerful means for the real-time monitoring of cultivated cells. It presents several key advantages, e.g., integration into standard incubator, compatibility with standard cell culture protocol, simplicity and ease of use. It can perform the follow-up in a large field of view (25 mm2) of several crucial parameters during the culture of cells i.e. their motility, their proliferation rate or their death. Consequently the setup can gather large statistics both in space and time. But in the case of tissue growth experiments, the field of view of 25 mm2 remains not sufficient and results can be biased depending on the position of the device with respect to the recipient of the cell culture. Hence, to conduct exhaustive wound healing assay, here we propose to enlarge the field of view up to 10 cm2 through two different approaches. The first method consists in performing a scan of the cell culture by moving the source/sensor couple and then stitch the stack of images. The second is to make an acquisition by scanning with a line scan camera. The two approaches are compared in term of resolution, complexity and acquisition time. Next we have performed acquisitions of wound healing assay (keratinocytes HaCaT) both in real-time (25 mm2) and in final point (10 cm2) to assess the combination of these two complementary modalities. In the future, we aim at combining directly super wide field of view acquisitions (>10 cm2) with real time ability inside the incubator.

Published
2015
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20. Wide-field lensfree imaging of tissue slides

Author

Morel, Sophie Nhu An, additional, Delon, Antoine, additional, Blandin, Pierre, additional, Bordy, Thomas, additional, Cioni, Olivier, additional, Hervé, Lionel, additional, Fromentin, Catherine, additional, Dinten, Jean-Marc, additional, and Allier, Cédric, additional

Published
2015
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30. Wide-field lensfree imaging of tissue slides

Author

Beaurepaire, Emmanuel, So, Peter T. C., Pavone, Francesco, Hillman, Elizabeth M., Morel, Sophie Nhu An, Delon, Antoine, Blandin, Pierre, Bordy, Thomas, Cioni, Olivier, Hervé, Lionel, Fromentin, Catherine, Dinten, Jean-Marc, and Allier, Cédric

Published
2015
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32. Coupling X-ray and optical tomography systems for in vivo examination of small animals.

Author

da Silva A, Bordy T, Debourdeau M, Dinten JM, Peltié P, and Rizo P

Subjects
Animals, Equipment Design, Equipment Failure Analysis, Mice, Microscopy, Fluorescence instrumentation, Microscopy, Fluorescence veterinary, Reproducibility of Results, Sensitivity and Specificity, Image Enhancement instrumentation, Tomography, Optical instrumentation, Tomography, Optical veterinary, Tomography, X-Ray Computed instrumentation, Tomography, X-Ray Computed veterinary, Whole Body Imaging instrumentation, Whole Body Imaging veterinary
Abstract

For the purpose of the co-registration of fluorescence optical signal and X-rays measurements, a multimodality tomographer has been developed in our laboratory. Such a system brings the possibility to get, on the same bench, in vivo both anatomical and functional information. Moreover, the information on the morphology of the animal can be used as a regularization factor in order to get the reconstructions of the biodistribution of fluorochromes more accurate and to reduce the computation time. A study on homogeneous phantoms was conducted to evaluate the feasibility, to test the linearity and the reproducibility, and to fix the parameters for the co-registration. More cumbersome phantoms (sacrificed mice) have then been considered and the test experiments were reproduced. Finally, results of a study conducted in vivo on mice bearing tumors in the lungs, tagged with different types of optical probes, are presented.

Published
2007
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