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Isolated boxwood endophytes have been demonstrated to effectively protect boxwood plants from infection by Calonectria pseudonaviculata (Cps). However, the roles of endophytes as communities in plant defense are not clear. Here, we demonstrated differential tolerance to Cps of English boxwood (Buxus sempervirens ‘Suffruticosa’), an iconic landscape plant and generally regarded as highly susceptible, and its link to endophyte complexity. Fifteen boxwood twig samples were collected in triplicates from three historic gardens—Colonial Williamsburg, George Washington’s Mount Vernon and River Farm, and Virginia Tech’s research farm in Virginia Beach in the summer and fall of 2019. A portion of individual samples was inoculated with Cps under controlled conditions. Significant differences in disease severity were observed among samples but not between the two seasons. Examining the endophyte cultures of the summer samples revealed that bacterial and fungal abundance was negatively and positively correlated with the disease severity. Nanopore metagenomics analysis on genomic DNA of the tolerant and susceptible group representatives confirmed the associations. Specifically, tolerant English boxwood plants had an endophyte community dominated by Bacilli and Betaproteobacteria, while susceptible ones had a distinct endophyte community dominated by Gammaproteobacteria, Actinobacteria, and diverse fungi. These findings may lead to boxwood health management innovations—devising and utilizing cultural practices to manipulate and increase the abundance and performance of beneficial endophytes for enhanced boxwood resistance to Cps.

Discusses farmers markets and the kinds of food crops sold at these markets. Notes a recent trend of more international specialty vegetables becoming available at these venues.

Provides a set of guidelines for home growers of landscape boxwood to avoid introduction of the boxwood blight pathogen into a landscape or, if the disease is already present in a landscape, to manage the disease in the most effective manner and avoid the spread of the disease to new locations.

Best management practices for boxwood blight (also called “box blight”) for greenery producers are practices recommended to avoid the introduction and spread of boxwood blight, caused by the fungus Calonectria pseudonaviculata. The recommendations in this document are designed to avoid spread of boxwood blight within a planting or to new locations when pruned tips are collected, sold and/or used for holiday greenery.

Provides a set of guidelines for home growers of landscape boxwood to avoid introduction of the boxwood blight pathogen into a landscape or, if the disease is already present in a landscape, to manage the disease in the most effective manner and avoid spread of the disease to new locations.