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359 results on '"Bonvicini, Francesca"'

5. Porous titanium scaffolds modified with Zeolitic Imidazolate Framework (ZIF-8) with enhanced osteogenic activity for the prevention of implant-associated infections.

Author

Di Matteo, Valentina, Di Filippo, Maria Francesca, Ballarin, Barbara, Bonvicini, Francesca, Iaquinta, Maria Rosa, Panzavolta, Silvia, Mazzoni, Elisa, Cassani, Maria Cristina, Liu, Jing, and Cabrera-Munguia, Denis A.

Subjects
BACTERIAL adhesion, INFECTION prevention, X-ray powder diffraction, TITANIUM, EMISSION spectroscopy, STAPHYLOCOCCUS epidermidis
Abstract

In this study, zeolitic imidazolate framework 8 (ZIF-8) was coated on porous Ti6Al4V scaffolds, either bare or previously modified using hydroxyapatite (HA) or HA and gelatin (HAgel), via a growing single-step method in aqueous media using two contact times at 6 h and 24 h. The coated scaffolds termed ZIF-8@Ti, ZIF-8@ HA/Ti, and ZIF-8@HAgel/Ti were characterized via scanning electron microscopy (SEM), powder X-ray diffraction (PXRD), attenuated total reflectance-Fourier transform infrared (ATR-FTIR), and molecular plasma-atomic emission spectroscopy (MP-AES). In order to assess the cell proliferation rate, the cytocompatibility of the scaffolds was evaluated in primary osteoblasts (hOBs) using alamarBlue assay, while the osteoconductivity was analyzed in hOBs using a real-time approach, evaluating the expression of secreted phosphoprotein 1 (SPP1). Osteopontin, which is the protein encoded by this gene, represents the major non-collagenous bone protein that binds tightly to HA. The scaffolds were shown to be non-cytotoxic based on hOB proliferation at all time points of analysis (24 h and 72 h). In hOB cultures, the scaffolds induced the upregulation of SPP1 with different fold changes. Some selected scaffolds were assayed in vitro for their antibacterial potential against Staphylococcus epidermidis; the scaffolds coated with ZIF-8 crystals, regardless of the presence of HA and gelatin, strongly inhibited bacterial adhesion to the materials and reduced bacterial proliferation in the culture medium, demonstrating the suitable release of ZIF-8 in a bioactive form. These experiments suggest that the innovative scaffolds, tested herein, provide a good microenvironment for hOB adhesion, viability, and osteoconduction with effective prevention of S. epidermidis adhesion. [ABSTRACT FROM AUTHOR]

Published
2024
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15. Cellulose acetate membranes loaded with combinations of tetraphenylporphyrin, graphene oxide and Pluronic F-127 as responsive materials with antibacterial photodynamic activity

Author

Morsi, Rania E., primary, Gentili, Denis, additional, Corticelli, Franco, additional, Morandi, Vittorio, additional, Figoli, Alberto, additional, Russo, Francesca, additional, Galiano, Francesco, additional, Gentilomi, Giovanna Angela, additional, Bonvicini, Francesca, additional, Manet, Ilse, additional, and Ventura, Barbara, additional

Published
2023
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16. Effectiveness of Snail Slime in the Green Synthesis of Silver Nanoparticles

Author

Di Filippo, Maria Francesca, primary, Di Matteo, Valentina, additional, Dolci, Luisa Stella, additional, Albertini, Beatrice, additional, Ballarin, Barbara, additional, Cassani, Maria Cristina, additional, Passerini, Nadia, additional, Gentilomi, Giovanna Angela, additional, Bonvicini, Francesca, additional, and Panzavolta, Silvia, additional

Published
2022
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28. Antibiotic resistance, virulence factors, phenotyping, and genotyping of non–escherichia coli enterobacterales from the gut microbiota of healthy subjects

Author

Amaretti, Alberto, Righini, Lucia, Candeliere, Francesco, Musmeci, Eliana, Bonvicini, Francesca, Gentilomi, Giovanna Angela, Rossi, Maddalena, Raimondi, Stefano, Amaretti A., Righini L., Candeliere F., Musmeci E., Bonvicini F., Gentilomi G.A., Rossi M., and Raimondi S.

Subjects
Virulence Factors, Antibiotic resistance, Enterobacter, Microbial Sensitivity Tests, Amoxicillin-Potassium Clavulanate Combination, Article, lcsh:Chemistry, Citrobacter, Enterobacteriaceae, Enterobacterale, Drug Resistance, Multiple, Bacterial, RNA, Ribosomal, 16S, Klebsiella, Humans, citrobacter, lcsh:QH301-705.5, Virulence, enterobacter, Biofilm, Healthy Volunteers, Anti-Bacterial Agents, Gastrointestinal Microbiome, Cronobacter, lcsh:Biology (General), lcsh:QD1-999, enterobacterales, klebsiella, Enterobacterales, beta-Lactamase Inhibitors
Abstract

Non-Escherichia coli Enterobacterales (NECE) can colonize the human gut and may present virulence determinants and phenotypes that represent severe heath concerns. Most information is available for virulent NECE strains, isolated from patients with an ongoing infection, while the commensal NECE population of healthy subjects is understudied. In this study, 32 NECE strains were isolated from the feces of 20 healthy adults. 16S rRNA gene sequencing and mass spectrometry attributed the isolates to Klebsiella pneumoniae, Klebsiella oxytoca, Enterobacter cloacae, Enterobacter aerogenes, Enterobacter kobei, Citrobacter freundii, Citrobacter amalonaticus, Cronobacter sp., and Hafnia alvei, Morganella morganii, and Serratia liquefaciens. Multiplex PCR revealed that K. pneumoniae harbored virulence genes for adhesins (mrkD, ycfM, and kpn) and enterobactin (entB) and, in one case, also for yersiniabactin (ybtS, irp1, irp2, and fyuA). Virulence genes were less numerous in the other NECE species. Biofilm formation was spread across all the species, while curli and cellulose were mainly produced by Citrobacter and Enterobacter. Among the most common antibiotics, amoxicillin-clavulanic acid was the sole against which resistance was observed, only Klebsiella strains being susceptible. The NECE inhabiting the intestine of healthy subjects have traits that may pose a health threat, taking into account the possibility of horizontal gene transfer.

Published
2020

30. Effect of Lactobacillus acidophilus Fermented Broths Enriched with Eruca sativa Seed Extracts on Intestinal Barrier and Inflammation in a Co-Culture System of an Enterohemorrhagic Escherichia coli and Human Intestinal Cells

Author

Bonvicini, Francesca, primary, Pagnotta, Eleonora, additional, Punzo, Angela, additional, Calabria, Donato, additional, Simoni, Patrizia, additional, Mirasoli, Mara, additional, Passerini, Nadia, additional, Bertoni, Serena, additional, Ugolini, Luisa, additional, Lazzeri, Luca, additional, Gentilomi, Giovanna Angela, additional, Caliceti, Cristiana, additional, and Roda, Aldo, additional

Published
2020
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32. A radiopaque calcium phosphate bone cement with long-lasting antibacterial effect: From paste to injectable formulation

Author

Di Filippo, Maria Francesca, primary, Dolci, Luisa Stella, additional, Albertini, Beatrice, additional, Passerini, Nadia, additional, Torricelli, Paola, additional, Parrilli, Annapaola, additional, Fini, Milena, additional, Bonvicini, Francesca, additional, Gentilomi, Giovanna Angela, additional, Panzavolta, Silvia, additional, and Bigi, Adriana, additional

Published
2020
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42. The relation between amyotrophic lateral sclerosis and inorganic selenium in drinking water: a population-based case-control study

Author

Rothman Kenneth J, Bonvicini Francesca, Vinceti Marco, Vescovi Luciano, and Wang Feiyue

Subjects
Industrial medicine. Industrial hygiene, RC963-969, Public aspects of medicine, RA1-1270
Abstract

Abstract Background A community in northern Italy was previously reported to have an excess incidence of amyotrophic lateral sclerosis among residents exposed to high levels of inorganic selenium in their drinking water. Methods To assess the extent to which such association persisted in the decade following its initial observation, we conducted a population-based case-control study encompassing forty-one newly-diagnosed cases of amyotrophic lateral sclerosis and eighty-two age- and sex-matched controls. We measured long-term intake of inorganic selenium along with other potentially neurotoxic trace elements. Results We found that consumption of drinking water containing ≥ 1 μg/l of inorganic selenium was associated with a relative risk for amyotrophic lateral sclerosis of 5.4 (95% confidence interval 1.1-26) after adjustment for confounding factors. Greater amounts of cumulative inorganic selenium intake were associated with progressively increasing effects, with a relative risk of 2.1 (95% confidence interval 0.5-9.1) for intermediate levels of cumulative intake and 6.4 (95% confidence interval 1.3-31) for high intake. Conclusion Based on these results, coupled with other epidemiologic data and with findings from animal studies that show specific toxicity of the trace element on motor neurons, we hypothesize that dietary intake of inorganic selenium through drinking water increases the risk for amyotrophic lateral sclerosis.

Published
2010
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44. Inhibition of Parvovirus B19 replication by Cidofovir in extendedly exposed erythroid progenitor cells

Author

Bonvicini, Francesca, Bua, Gloria, Conti, Ilaria, Manaresi, Elisabetta, Gallinella, Giorgio, Bonvicini, Francesca, Bua, Gloria, Conti, Ilaria, Manaresi, Elisabetta, and Gallinella, Giorgio

Subjects
viruses, virus diseases, Parvovirus B19, Antiviral activity, Cidofovir, NO
Abstract

Human parvovirus B19 (B19V) commonly induces self-limiting infections but can also cause severe clinical manifestations in patients with underlying hematological disorders or with immune system deficits. Currently, therapeutic options for B19V entirely rely on symptomatic and supportive treatments since a specific antiviral therapy is not yet available. Recently a first step in the research for active compounds inhibiting B19V replication has allowed identifying the acyclic nucleoside phosphonate cidofovir (CDV). Herein, the effect of CDV against B19V replication was characterized in human erythroid progenitor cells (EPCs) cultured and infected following different experimental approaches to replicate in vitro the infection of an expanding erythroid cell population in the bone marrow. B19V replication was selectively inhibited both in infected EPCs extendedly exposed to CDV 500 μM (viral inhibition 82%) and in serially infected EPCs cultures with passages of the viral progeny, constantly under drug pressure (viral inhibition >99%). In addition, a potent inhibitory effect against B19V (>92%) was assessed in a short-term infection of EPCs treated with CDV 500 μM prior to viral infection. This enhanced anti-B19V activity of CDV could be ascribed both to the increased intracellular drug concentration achieved by extended exposure, and to a progressive reduction in efficiency of the replicative process within treated EPCs population.

Published
2016

45. Inhibitory effect of Cidofovir on Parvovirus B19 replication

Author

BUA, GLORIA, BONVICINI, FRANCESCA, MANARESI, ELISABETTA, GALLINELLA, GIORGIO, Bua, Gloria, Bonvicini, Francesca, Manaresi, Elisabetta, and Gallinella, Giorgio

Subjects
viruses
Abstract

Parvovirus B19 (B19V) is a human ssDNA virus responsible for a wide range of clinical manifestations, still lacking for a specific antiviral therapy. The identification of compounds active against B19V may add therapeutic options to the treatment of B19V infections. To this purpose, focus was raised to cidofovir (CDV), an acyclic nucleoside phosphonate broadly active against dsDNA viruses. Two model systems were used to assess the inhibitory effect of CDV on B19V replication: 1) the UT7/EpoS1 megakaryoblastoid cell line, able to support B19V DNA replication; 2) the ex vivo expanded CD36+ erythroid progenitor cells (EPCs), a highly permissive system for B19V replication and expression. EPCs were generated from peripheral blood, via culture in a medium containing erythropoietic growth factors (EPO, SCF and Il-3), for up to 18 days. Expression of EPC differentiation markers ranged from a minimum of 10% at 3-day to a 80% at 15-day culturing. Infection at different stages indicated that the EPC system was fully permissive to B19V between days 6 and 15 of EPCs in vitro growth and differentiation. Experiments were carried out at different multiplicity of infection (100 – 104 genomes/cell) and CDV concentrations (0.1 – 500 μM). The effects of CDV were evaluated by its capacity to inhibit viral nucleic acid synthesis, as determined by means of q PCR assays for quantification of viral nucleic acids. CDV showed a dose-dependent inhibiting activity on B19V replication within infected UT7/ EpoS1, allowing for the determination of EC50 and EC90 values (7.45–41.27 μM, and 84.73–360.7 μM, respectively). In EPCs, a significant reduction on B19V DNA amounts was obtained only at 500 μM (68.2–92.8%). However, cell-culture supernatants from B19V-infected, CDV-treated EPCs were used for serial infection of EPCs in the presence of CDV, leading to a progressive inhibition of B19V replication compared to untreated controls. With regard to the host cells, the drug did not interfere with the overall cellular DNA replication and metabolic activity. The effect of CDV on B19V could be likely related to a specific inhibition on the viral replication process, indicating the possibility of developing an antiviral strategy against a relevant human pathogenic virus.

Published
2015

46. Widening the Therapeutic Perspectives of Clofazimine by Its Loading in Sulfobutylether β-Cyclodextrin Nanocarriers: Nanomolar IC50 Values against MDR S. epidermidis

Author

Wankar, Jitendra, primary, Bonvicini, Francesca, additional, Benkovics, Gabor, additional, Marassi, Valentina, additional, Malanga, Milo, additional, Fenyvesi, Eva, additional, Gentilomi, Giovanna Angela, additional, Reschiglian, Pierluigi, additional, Roda, Barbara, additional, and Manet, Ilse, additional

Published
2018
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