1. Vibrational microspectroscopy as a tool to unveil new chemotherapeutic strategies against osteosarcoma.
- Author
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Laginha RC, Silva JD, Cinque G, Batista de Carvalho LAE, and Batista de Carvalho ALM
- Subjects
- Humans, Cell Line, Tumor, Spectroscopy, Fourier Transform Infrared methods, Vibration, Spermine pharmacology, Spermine chemistry, Bone Neoplasms drug therapy, Bone Neoplasms pathology, Bone Neoplasms metabolism, Spermidine pharmacology, Spermidine chemistry, Principal Component Analysis, Cell Survival drug effects, Osteosarcoma drug therapy, Osteosarcoma pathology, Osteosarcoma metabolism, Spectrum Analysis, Raman methods, Antineoplastic Agents pharmacology, Antineoplastic Agents chemistry
- Abstract
Over the years, osteosarcoma therapy has had a significative improvement with the use of a multidrug regime strategy, increasing the survival rates from less than 20 % to circa 70 %. Different types of development of new antineoplastic agents are critical to achieve irreversible damage to cancer cells, while preserving the integrity of their healthy counterparts. In the present study, complexes with two and three Pd(II) centres linked by the biogenic polyamines: spermine (Pd
2 SpmCl4 ) and spermidine (Pd3 Spd2 Cl6 ) were tested against non-malignant (osteoblasts, HOb) and cancer (osteosarcoma, MG-63) human cell lines. Either alone or in combination according to the EURAMOS-1 protocol, they were used versus cisplatin as a drug reference. By evaluating the cytotoxic effects of both therapeutic approaches (single and drug combination) in HOb and MG-63 cell lines, the selective anti-tumoral potential is assessed. To understand the different treatments at a molecular level, Synchrotron Radiation Fourier Transform Infrared and Raman microspectroscopies were applied. Principal component analysis and hierarchical cluster analysis are applied to the vibrational data, revealing the major metabolic changes caused by each drug, which were found to rely on DNA, lipids, and proteins, acting as biomarkers of drug-to-cell impact. The main changes were observed for the B-DNA native conformation to either Z-DNA (higher in the presence of polynuclear complexes) or A-DNA (preferably after cisplatin exposure). Additionally, a higher effect upon variation in proteins content was detected in drug combination when compared to single drug administration proving the efficacy of the EURAMOS-1 protocol with the new drugs tested., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.)- Published
- 2024
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