Search

Your search keyword '"Bolotin-Fukuhara, Monique"' showing total 230 results
Start Over Author "Bolotin-Fukuhara, Monique"
230 results on '"Bolotin-Fukuhara, Monique"'

11. Functional networks of co-expressed genes to explore iron homeostasis processes in the pathogenic yeast Candida glabrata

Author

Denecker, Thomas, Zhou Li, Youfang, Fairhead, Cécile, Budin, Karine, Camadro, Jean-Michel, Bolotin-Fukuhara, Monique, Angoulvant, Adela, Lelandais, Gaëlle, Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Génétique Quantitative et Evolution - Le Moulon (Génétique Végétale) (GQE-Le Moulon), AgroParisTech-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut Jacques Monod (IJM (UMR_7592)), Université de Paris (UP)-Centre National de la Recherche Scientifique (CNRS), and Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)

Subjects
Genetics, 0303 health sciences, Candida glabrata, 030306 microbiology, [SDV]Life Sciences [q-bio], Standard Article, Biology, biology.organism_classification, 3. Good health, Ferrous, Transcriptome, Functional networks, 03 medical and health sciences, Adaptation, Pathogen, Gene, Homeostasis, 030304 developmental biology
Abstract

Candida glabrata is a cause of life-threatening invasive infections especially in elderly and immunocompromised patients. Part of human digestive and urogenital microbiota, C. glabrata faces varying iron availability, low during infection or high in digestive and urogenital tracts. To maintain its homeostasis, C. glabrata must get enough iron for essential cellular processes and resist toxic iron excess. The response of this pathogen to both depletion and lethal excess of iron at 30°C have been described in the literature using different strains and iron sources. However, adaptation to iron variations at 37°C, the human body temperature and to gentle overload, is poorly known. In this study, we performed transcriptomic experiments at 30°C and 37°C with low and high but sub-lethal ferrous concentrations. We identified iron responsive genes and clarified the potential effect of temperature on iron homeostasis. Our exploration of the datasets was facilitated by the inference of functional networks of co-expressed genes, which can be accessed through a web interface. Relying on stringent selection and independently of existing knowledge, we characterized a list of 214 genes as key elements of C. glabrata iron homeostasis and interesting candidates for medical applications.

Published
2020
Full Text
View/download PDF

12. Genome evolution in yeasts

Author

Dujon, Bernard, Sherman, David, Fischer, Gilles, Durrens, Pascal, Casaregola, Serge, Lafontaine, Ingrid, de Montigny, Jacky, Marck, Christian, Neuveglise, Cecile, Talla, Emmanuel, Goffard, Nicolas, Frangeul, Lionel, Aigle, Michel, Anthouard, Veronique, Babour, Anna, Barbe, Valerie, Barnay, Stephanie, Blanchin, Sylvie, Beckerich, Jean-Marie, Beyne, Emmanuelle, Bleykasten, Claudine, Boisrame, Anita, Boyer, Jeanne, Cattolico, Laurence, Confanioleri, Fabrice, de Daruvar, Antoine, Despons, Laurence, Fabre, Emmanuelle, Fairhead, Cecile, Ferry-Dumazet, Helene, Groppi, Alexis, Hantraye, Florence, Hennequin, Christophe, Jauniaux, Nicolas, Joyet, Philippe, Kachouri, Rym, Kerrest, Alix, Koszul, Romain, Lemaire, Marc, Lesur, Isabelle, Ma, Laurence, Muller, Heloise, Nicaud, Jean-Marc, Nikolski, Macha, Oztas, Sophie, Ozier-Kalogeropoulos, Odile, Pellenz, Stefan, Potier, Serge, Richard, Guy-Franck, Straub, Marie-Laure, Suleau, Audrey, Swennen, Dominique, Tekaia, Fredj, Wesolowski-Louvel, Micheline, Westhof, Eric, Wirth, Benedicte, Zeniou-Meyer, Maria, Zivanovic, Ivan, Bolotin-Fukuhara, Monique, Thierry, Agnes, Bouchier, Christiane, Caudron, Bernard, Scarpelli, Claude, Gaillardin, Claude, Weissenbach, Jean, Wincker, Patrick, and Souciet, Jean-Luc

Subjects
Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

Author(s): Bernard Dujon (corresponding author) [1]; David Sherman [5, 6]; Gilles Fischer [1]; Pascal Durrens [6, 7]; Serge Casaregola [8]; Ingrid Lafontaine [1]; Jacky de Montigny [9]; Christian Marck [10]; [...]

Published
2004
Full Text
View/download PDF

13. Linear versus circular mitochondrial genomes: intraspecies variability of mitochondrial genome architecture in Candida parapsilosis

Author

Rycovska, Adriana, Valach, Matus, Tomaska, Lubomir, Bolotin-Fukuhara, Monique, and Nosek, Jozef

Subjects
Genomes -- Research, Mitochondria -- Genetic aspects, Mitochondria -- Research, Candida -- Research, Candida -- Genetic aspects, Biological sciences
Abstract

The yeast species Candida parapsilosis, an opportunistic pathogen, exhibits genetic and genomic heterogeneity. To assess the polymorphism at the level of mitochondrial DNA (mtDNA), the organization of the mitochondrial genome in strains belonging to the three variant groups of this species was investigated. Although these analyses revealed a group-specific restriction fragment pattern of mtDNA, strains belonging to different groups appear to have similar genes in the same gene order. An extensive survey of C. parapsilosis isolates uncovered surprising alterations in the molecular architecture of their mitochondrial genome. A screening strategy for strains harbouring mtDNA with rearranged architecture showed that nearly all strains from groups I and III possess linear mtDNA molecules terminating with arrays of tandem repeat units, while most of the group II strains have a circular mitochondrial genome. In addition, it was found that linear genophores in mitochondria of strains from different groups differ in the sequence of the mitochondrial telomeric repeat unit. The occurrence of altered forms of mtDNA among C. parapsilosis strains opens up the unique possibility to address questions concerning the evolutionary origin and replication strategy of linear and circular genomes in mitochondria.

Published
2004

20. Glucose uptake in Kluyveromyces lactis: role of the HGT1 gene in glucose transport

Author

Billard, Patrick, Menart, Sandrine, Blaisonneau, Joel, Bolotin-Fukuhara, Monique, Fukuhara, Hiroshi, and Wesolowski-Louvel, Micheline

Subjects
Glucose metabolism -- Genetic aspects, Yeast -- Genetic aspects, Gene expression -- Research, Biological sciences
Abstract

Nucleotide sequence of the HGT1, a gene for high-affinity glucose transport in the lactose-assimilating yeast Kluyveromyces lactis, indicates that it encodes a typical transmembrane protein with 12 hydrophobic domains and with 26% to 31% amino acid identity with the Hxtp family of glucose transport elements in Saccharomyces cerevisiae. In contrast to RAG1, the major gene for low-affinity glucose uptake in K. lactis, expression is constitutive and controlled by a number of genes also known to influence expression of RAG1. A large reduction in glucose uptake at low concentrations was seen in disruption strains and growth was affected in low-glucose medium.

Published
1996

24. Iron homeostasisis in C. glabrata at the transcriptomic level

Author

Zhou-li, Youfang, Denecker, Thomas, Budin, Karine, Fairhead, Cécile, Bolotin Fukuhara, Monique, Boisnard, Stéphanie, Lelandais, Gaëlle, Angoulvant, Adéla, Génétique Quantitative et Evolution - Le Moulon (Génétique Végétale) (GQE-Le Moulon), Centre National de la Recherche Scientifique (CNRS)-AgroParisTech-Université Paris-Sud - Paris 11 (UP11)-Institut National de la Recherche Agronomique (INRA), Université Paris-Sud - Paris 11 (UP11), and ProdInra, Migration

Subjects
[SDV.MP.MYC] Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, ComputingMilieux_MISCELLANEOUS, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology
Abstract

International audience

Published
2018
Full Text
View/download PDF

26. Tipping the balance both ways: drug resistance and virulence in Candida glabrata

Author

Vale-Silva, Luis A., Sanglard, Dominique, and Bolotin-Fukuhara, Monique

Abstract

Among existing fungal pathogens, Candida glabrata is outstanding in its capacity to rapidly develop resistance to currently used antifungal agents. Resistance to the class of azoles, which are still widely used agents, varies in proportion (from 5 to 20%) depending on geographical area. Moreover, resistance to the class of echinocandins, which was introduced in the late 1990s, is rising in several institutions. The recent emergence of isolates with acquired resistance to both classes of agents is a major concern since alternative therapeutic options are scarce. Although considered less pathogenic than C. albicans, C. glabrata has still evolved specific virulence traits enabling its survival and propagation in colonized and infected hosts. Development of drug resistance is usually associated with fitness costs, and this notion is documented across several microbial species. Interestingly, azole resistance in C. glabrata has revealed the opposite. Experimental models of infection showed enhanced virulence of azole-resistant isolates. Moreover, azole resistance could be associated with specific changes in adherence properties to epithelial cells or innate immunity cells (macrophages), both of which contribute to virulence changes. Here we will summarize the current knowledge on C. glabrata drug resistance and also discuss the consequences of drug resistance acquisition on the balance between C. glabrata and its hosts

Published
2017

28. Genomic Exploration of the Hemiascomycetous Yeasts: 21. Comparative functional classification of genes

Author

Gaillardin, Claude, Duchateau-Nguyen, Guillemette, Tekaia, Fredj, Llorente, Bertrand, Casaregola, Serge, Toffano-Nioche, Claire, Aigle, Michel, Artiguenave, François, Blandin, Gaëlle, Bolotin-Fukuhara, Monique, Bon, Elisabeth, Brottier, Philippe, de Montigny, Jacky, Dujon, Bernard, Durrens, Pascal, Lépingle, Andrée, Malpertuy, Alain, Neuvéglise, Cécile, Ozier-Kalogéropoulos, Odile, Potier, Serge, Saurin, William, Termier, Michel, Wésolowski-Louvel, Micheline, Wincker, Patrick, Souciet, Jean-Luc, and Weissenbach, Jean

Published
2000
Full Text
View/download PDF

29. Genomic Exploration of the Hemiascomycetous Yeasts: 19. Ascomycetes-specific genes

Author

Malpertuy, Alain, Tekaia, Fredj, Casarégola, Serge, Aigle, Michel, Artiguenave, Francois, Blandin, Gaëlle, Bolotin-Fukuhara, Monique, Bon, Elisabeth, Brottier, Philippe, de Montigny, Jacky, Durrens, Pascal, Gaillardin, Claude, Lépingle, Andrée, Llorente, Bertrand, Neuvéglise, Cécile, Ozier-Kalogeropoulos, Odile, Potier, Serge, Saurin, William, Toffano-Nioche, Claire, Wésolowski-Louvel, Micheline, Wincker, Patrick, Weissenbach, Jean, Souciet, Jean-Luc, and Dujon, Bernard

Published
2000
Full Text
View/download PDF

30. Genomic Exploration of the Hemiascomycetous Yeasts: 20. Evolution of gene redundancy compared to Saccharomyces cerevisiae

Author

Llorente, Bertrand, Durrens, Pascal, Malpertuy, Alain, Aigle, Michel, Artiguenave, François, Blandin, Gaëlle, Bolotin-Fukuhara, Monique, Bon, Elisabeth, Brottier, Philippe, Casaregola, Serge, Dujon, Bernard, de Montigny, Jacky, Lépingle, Andrée, Neuvéglise, Cécile, Ozier-Kalogeropoulos, Odile, Potier, Serge, Saurin, William, Tekaia, Fredj, Toffano-Nioche, Claire, Wésolowski-Louvel, Micheline, Wincker, Patrick, Weissenbach, Jean, Souciet, Jean-Luc, and Gaillardin, Claude

Published
2000
Full Text
View/download PDF

31. Genomic Exploration of the Hemiascomycetous Yeasts: 18. Comparative analysis of chromosome maps and synteny with Saccharomyces cerevisiae

Author

Llorente, Bertrand, Malpertuy, Alain, Neuvéglise, Cécile, de Montigny, Jacky, Aigle, Michel, Artiguenave, François, Blandin, Gaëlle, Bolotin-Fukuhara, Monique, Bon, Elisabeth, Brottier, Philippe, Casaregola, Serge, Durrens, Pascal, Gaillardin, Claude, Lépingle, Andrée, Ozier-Kalogéropoulos, Odile, Potier, Serge, Saurin, William, Tekaia, Fredj, Toffano-Nioche, Claire, Wésolowski-Louvel, Micheline, Wincker, Patrick, Weissenbach, Jean, Souciet, Jean-Luc, and Dujon, Bernard

Published
2000
Full Text
View/download PDF

32. Genomic Exploration of the Hemiascomycetous Yeasts: 3. Methods and strategies used for sequence analysis and annotation

Author

Tekaia, Fredj, Blandin, Gaëlle, Malpertuy, Alain, Llorente, Bertrand, Durrens, Pascal, Toffano-Nioche, Claire, Ozier-Kalogeropoulos, Odile, Bon, Elisabeth, Gaillardin, Claude, Aigle, Michel, Bolotin-Fukuhara, Monique, Casarégola, Serge, de Montigny, Jacky, Lépingle, Andrée, Neuvéglise, Cécile, Potier, Serge, Souciet, Jean-Luc, Wésolowski-Louvel, Micheline, and Dujon, Bernard

Published
2000
Full Text
View/download PDF

33. Genomic Exploration of the Hemiascomycetous Yeasts: 1. A set of yeast species for molecular evolution studies

Author

Souciet, Jean-Luc, Aigle, Michel, Artiguenave, François, Blandin, Gaëlle, Bolotin-Fukuhara, Monique, Bon, Elisabeth, Brottier, Philippe, Casaregola, Serge, de Montigny, Jacky, Dujon, Bernard, Durrens, Pascal, Gaillardin, Claude, Lépingle, Andrée, Llorente, Bertrand, Malpertuy, Alain, Neuvéglise, Cécile, Ozier-Kalogéropoulos, Odile, Potier, Serge, Saurin, William, Tekaia, Fredj, Toffano-Nioche, Claire, Wésolowski-Louvel, Micheline, Wincker, Patrick, and Weissenbach, Jean

Published
2000
Full Text
View/download PDF

34. Genomic Exploration of the Hemiascomycetous Yeasts: 4. The genome of Saccharomyces cerevisiae revisited

Author

Blandin, Gaëlle, Durrens, Pascal, Tekaia, Fredj, Aigle, Michel, Bolotin-Fukuhara, Monique, Bon, Elisabeth, Casarégola, Serge, de Montigny, Jacky, Gaillardin, Claude, Lépingle, Andrée, Llorente, Bertrand, Malpertuy, Alain, Neuvéglise, Cécile, Ozier-Kalogeropoulos, Odile, Perrin, Arnaud, Potier, Serge, Souciet, Jean-Luc, Talla, Emmanuel, Toffano-Nioche, Claire, Wésolowski-Louvel, Micheline, Marck, Christian, and Dujon, Bernard

Published
2000
Full Text
View/download PDF

35. Dissection of quantitative traits by bulk segregant mapping in a protoploid yeast species

Author

Sigwalt, Anastasie, Caradec, Claudia, Brion, Christian, Hou, Jing, De Montigny, Jacky, Jung, Paul, Fischer, Gilles, Llorente, Bertrand, Friedrich, Anne, Schacherer, Joseph, Bolotin-Fukuhara, Monique, Génétique moléculaire, génomique, microbiologie (GMGM), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Sciences Pour l'Oenologie (SPO), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université Montpellier 1 (UM1)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA), Université de Strasbourg (UNISTRA), Biology of Genomes [LCQB] (LCQB-BiG), Biologie Computationnelle et Quantitative = Laboratory of Computational and Quantitative Biology (LCQB), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche en Cancérologie de Marseille (CRCM), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Aix Marseille Université (AMU), Laboratoire d'Hydrologie et de Géochimie de Strasbourg (LHyGeS), École Nationale du Génie de l'Eau et de l'Environnement de Strasbourg (ENGEES)-Université de Strasbourg (UNISTRA)-Institut national des sciences de l'Univers (INSU - CNRS)-Ecole et Observatoire des Sciences de la Terre (EOST), Université de Strasbourg (UNISTRA)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Génétique Quantitative et Evolution - Le Moulon (Génétique Végétale) (GQE-Le Moulon), Centre National de la Recherche Scientifique (CNRS)-AgroParisTech-Université Paris-Sud - Paris 11 (UP11)-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Ecole et Observatoire des Sciences de la Terre (EOST), Université de Strasbourg (UNISTRA)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École Nationale du Génie de l'Eau et de l'Environnement de Strasbourg (ENGEES)-Centre National de la Recherche Scientifique (CNRS), Université Montpellier 1 (UM1)-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Aix Marseille Université (AMU)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg (UNISTRA)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École Nationale du Génie de l'Eau et de l'Environnement de Strasbourg (ENGEES)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), École Nationale du Génie de l'Eau et de l'Environnement de Strasbourg (ENGEES)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Ecole et Observatoire des Sciences de la Terre (EOST), and Université de Strasbourg (UNISTRA)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0301 basic medicine, BSA-seq, Lachancea kluyveri, [SDV]Life Sciences [q-bio], Saccharomyces cerevisiae, Quantitative Trait Loci, trait mapping, halotolerance, Quantitative trait locus, quantitative traits, Applied Microbiology and Biotechnology, Microbiology, 03 medical and health sciences, 0302 clinical medicine, Gene mapping, Genes, Reporter, Saccharomycotina, ComputingMilieux_MISCELLANEOUS, Genetics, biology, Staining and Labeling, Chromosome Mapping, General Medicine, biology.organism_classification, Phenotype, Yeast, Genetic architecture, 030104 developmental biology, Saccharomycetales, 030217 neurology & neurosurgery
Abstract

Since more than a decade ago, Saccharomyces cerevisiae has been used as a model to dissect complex traits, revealing the genetic basis of a large number of traits in fine detail. However, to have a more global view of the genetic architecture of traits across species, the examination of the molecular basis of phenotypes within non-conventional species would undoubtedly be valuable. In this respect, the Saccharomycotina yeasts represent ideal and potential non-model organisms. Here we sought to assess the feasibility of genetic mapping by bulk segregant analysis in the protoploid Lachancea kluyveri (formerly S. kluyveri ) yeast species, a distantly related species to S. cerevisiae . For this purpose, we designed a fluorescent mating-type marker, compatible with any mating-competent strains representative of this species, to rapidly create a large population of haploid segregants (>105 cells). Quantitative trait loci can be mapped by selecting and sequencing an enriched pool of progeny with extreme phenotypic values. As a test bed, we applied this strategy and mapped the causal loci underlying halotolerance phenotypes in L. kluyveri . Overall, this study demonstrates that bulk segregant mapping is a powerful way for investigating the genetic basis of natural variations in non-model yeast organisms and more precisely in L. kluyveri .

Published
2016
Full Text
View/download PDF

36. Efficient mating-type switching in Candida glabrata induces cell death

Author

Boisnard, Stéphanie, Zhou Li, Youfang, Arnaise, Sylvie, Sequeira, Gregory, Raffoux, Xavier, Enache-Angoulvant, Adela, Bolotin-Fukuhara, Monique, Fairhead, Cécile, Génétique Quantitative et Evolution - Le Moulon (Génétique Végétale) (GQE-Le Moulon), Centre National de la Recherche Scientifique (CNRS)-AgroParisTech-Université Paris-Sud - Paris 11 (UP11)-Institut National de la Recherche Agronomique (INRA), Institut de génétique et microbiologie [Orsay] (IGM), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), and CNRS and University Paris-Sud

Subjects
Cell Death, [SDV]Life Sciences [q-bio], lcsh:R, lcsh:Medicine, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Candida glabrata, lcsh:Q, Genes, Mating Type, Fungal, lcsh:Science, Research Article
Abstract

International audience; Candida glabrata is an apparently asexual haploid yeast that is phylogenetically closer to Saccharomyces cerevisiae than to Candida albicans. Its genome contains three MAT-like cassettes, MAT, which encodes either MATa or MATalpha information in different strains, and the additional loci, HML and HMR. The genome also contains an HO gene homolog, but this yeast has never been shown to switch mating-types spontaneously, as S. cerevisiae does. We have recently sequenced the genomes of the five species that, together with C. glabrata, make up the Nakaseomyces clade. All contain MAT-like cassettes and an HO gene homolog. In this work, we express the HO gene of all Nakaseomyces and of S. cerevisiae in C. glabrata. All can induce mating-type switching, but, despite the larger phylogenetic distance, the most efficient endonuclease is the one from S. cerevisiae. Efficient matingtype switching in C. glabrata is accompanied by a high cell mortality, and sometimes results in conversion of the additional cassette HML. Mortality probably results from the cutting of the HO recognition sites that are present, in HML and possibly HMR, contrary to what happens naturally in S. cerevisiae. This has implications in the life-cycle of C. glabrata, as we show that efficient MAT switching is lethal for most cells, induces chromosomal rearrangements in survivors, and that the endogenous HO is probably rarely active indeed.

Published
2015
Full Text
View/download PDF

40. Comparative genomics of the Nakaseomyces clade: Candida glabrata and enew merging pathogens

Author

Fairhead, Cécile, Gabaldón, Toni, Martin, Tiphaine, Durrens, Pascal, Lespinet, Olivier, Hennequin, Christophe, Bolotin-Fukuhara, Monique, Wincker, Patrick, Institut de génétique et microbiologie [Orsay] (IGM), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Center for Genomic Regulation (CRG-UPF), CIBER de Epidemiología y Salud Pública (CIBERESP), Models and Algorithms for the Genome ( MAGNOME), Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB)-Inria Bordeaux - Sud-Ouest, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria), Modèles et algorithmes pour la Bioinformatique et la Visualisation d'informations, (MABIOVIS), Laboratoire Bordelais de Recherche en Informatique (LaBRI), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB), Service Parasitologie-Mycologie, Hôpital St. Antoine, Immunité et Infection, Université Pierre et Marie Curie - Paris 6 (UPMC)-IFR113-Institut National de la Santé et de la Recherche Médicale (INSERM), Genoscope - Centre national de séquençage [Evry] (GENOSCOPE), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR113-Université Pierre et Marie Curie - Paris 6 (UPMC), Inria Bordeaux - Sud-Ouest, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), Université de Bordeaux (UB)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Centre National de la Recherche Scientifique (CNRS), Université de Bordeaux (UB)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Centre National de la Recherche Scientifique (CNRS), CHU Saint-Antoine [AP-HP], and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)

Subjects
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
Abstract

All authors wish to acknowledge the work of colleagues and collaborators who are authors of the final paper in preparation; International audience; C. glabrata has become the second fungal pathogenic agent of human illness after C. albicans, and two new species, C. nivariensis and C. bracarensis, have been described as emerging pathogens from the clade of the Nakaseomyces. We have sequenced these genomes and that of the other three environmental yeasts from the clade. These species share with Saccharomyces the common ancestor which underwent a whole genome duplication. Nonetheless, chromosome number is highly variable in the clade with some low chromosome numbers. Centromeres show less conservation than expected, as do telomeric repeats. All species share genomic and physiological features that define their monophylogeny. These include genome size and gene number, the presence of insertions in non coding RNA genes, loss of genes from metabolic pathways such as GAL and BNA, and absence of active transposable elements. All contain homologs of genes involved in mating, although most species are described as asexual, and all contain a putative HO gene, enabling mating-type switching in Saccharomyces. Indeed, sexual species in the clade are homothallic and may share with S. cerevisiae the HO gene and three duplicated cassettes. Nonetheless, variations are observed in the chromosomal structure of the cassettes. Other features are common only to C. glabrata and closest species. These include duplicated genes encoding ribosomal proteins, for example. We will report on gene family expansions and contractions in species, on specific features of pathogens, and on specificities of C. glabrata, such as tandem amplification of genes involved in virulence.

Published
2011

42. Yeasts as a model for human diseases

Author

Bolotin-Fukuhara, Monique, Dumas, Bruno, Gaillardin, Claude, Institut de génétique et microbiologie [Orsay] (IGM), and Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Kluyveromyces, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Humans, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Disease, Saccharomyces cerevisiae, Models, Biological, ComputingMilieux_MISCELLANEOUS, Pichia
Abstract

International audience

Published
2010
Full Text
View/download PDF

46. Genome evolution:lessons from genolevures

Author

Casaregola, Serge, Bolotin-Fukuhara, Monique, Aigle, Michel, Microbiologie et Génétique Moléculaire (MGM), Institut National de la Recherche Agronomique (INRA)-Institut National Agronomique Paris-Grignon (INA P-G)-Centre National de la Recherche Scientifique (CNRS), Institut de génétique et microbiologie [Orsay] (IGM), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Unité de Microbiologie et génétique (UMG), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon

Subjects
phylogénétique, genomique, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, levures, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2006

47. The Kluyveromyces lactis repertoire of transcriptional regulators

Author

Bussereau, Françoise, Casaregola, Serge, Lafay, Jean-François, Bolotin-Fukuhara, Monique, Institut de génétique et microbiologie [Orsay] (IGM), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Beckerich, Jean-Marie, Microbiologie et Génétique Moléculaire (MGM), and Institut National de la Recherche Agronomique (INRA)-Institut National Agronomique Paris-Grignon (INA P-G)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], evolution, genomics, [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, regulation, [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology
Published
2006

48. Functional Study of the Hap4-Like Genes Suggests That the Key Regulators of Carbon Metabolism HAP4 and Oxidative Stress Response YAP1 in Yeast Diverged from a Common Ancestor

Author

Petryk, Nataliya, primary, Zhou, You-Fang, additional, Sybirna, Kateryna, additional, Mucchielli, Marie-Hélène, additional, Guiard, Bernard, additional, Bao, Wei-Guo, additional, Stasyk, Oleh V., additional, Stasyk, Olena G., additional, Krasovska, Olena S., additional, Budin, Karine, additional, Reymond, Nancie, additional, Imbeaud, Sandrine, additional, Coudouel, Sophie, additional, Delacroix, Hervé, additional, Sibirny, Andriy, additional, and Bolotin-Fukuhara, Monique, additional

Published
2014
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results