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2. Improved human islets’ viability and functionality with mesenchymal stem cells and arg-gly-asp tripeptides supplementation of alginate micro-encapsulated islets in vitro

Author

Laporte, Camille, primary, Tubbs, Emily, additional, Pierron, Maxime, additional, Gallego, Amanda, additional, Moisan, Anaïck, additional, Lamarche, Frédéric, additional, Lozano, Tamara, additional, Hernandez, Andrea, additional, Cottet-Rousselle, Cécile, additional, Gauchez, Anne-Sophie, additional, Persoons, Virginie, additional, Bottausci, Frédéric, additional, Fontelaye, Caroline, additional, Boizot, François, additional, Lablanche, Sandrine, additional, and Rivera, Florence, additional

Published
2020
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3. A stand-alone and portable microfluidic platform for quantitative immunoassays with an integrated calibration

Author

Parent, Charlotte, Verplanck, Nicolas, Achard, Jean-Luc, Cubizolles, Myriam, Nonglaton, Guillaume, Boizot, François, Alessio, Manuel, Charles, Raymond, Fouillet, Yves, Département Microtechnologies pour la Biologie et la Santé (DTBS), Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Laboratoire des Écoulements Géophysiques et Industriels [Grenoble] (LEGI ), Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), and ACHARD, Jean-Luc

Subjects
[SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SPI.MECA.MSMECA] Engineering Sciences [physics]/Mechanics [physics.med-ph]/Materials and structures in mechanics [physics.class-ph], [SPI.MECA.MEFL] Engineering Sciences [physics]/Mechanics [physics.med-ph]/Fluids mechanics [physics.class-ph], [SPI.MECA.MSMECA]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Materials and structures in mechanics [physics.class-ph], ComputingMilieux_MISCELLANEOUS, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, [SPI.MECA.MEFL]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Fluids mechanics [physics.class-ph]
Abstract

International audience

Published
2018

4. Separation of Biological Particles in a Modular Platform of Cascaded Deterministic Lateral Displacement Modules

Author

Vincent Agache, Aurélie Thuaire, Frederic Revol-Cavalier, Boizot François, Charlotte Parent, Yves Fouillet, Nicolas Verplanck, Eloise Pariset, European Commission, Bpifrance, Pariset, Eloise, Département Microtechnologies pour la Biologie et la Santé (DTBS), Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Service Bio-System on Chip (SBSC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), FET Proactive H2020 Viruscan project (No. 731868). BactiDIAG grant funded by BPI France under the FUI framework (FUI-AAP18)., and Pariset, Eloise [0000-0002-9375-2361]

Subjects
0301 basic medicine, Male, Materials science, Fabrication, Erythrocytes, lcsh:Medicine, 02 engineering and technology, Cell Separation, Article, 03 medical and health sciences, [SPI]Engineering Sciences [physics], Cell Line, Tumor, Escherichia coli, Humans, Sample preparation, Particle Size, lcsh:Science, Multidisciplinary, business.industry, Dynamic range, lcsh:R, Sorting, Prostatic Neoplasms, Modular design, Microfluidic Analytical Techniques, 021001 nanoscience & nanotechnology, Sample (graphics), 030104 developmental biology, PC-3 Cells, Erythrocyte Count, Particle, lcsh:Q, Particle size, 0210 nano-technology, business, Biological system
Abstract

Deterministic lateral displacement (DLD) has been extensively implemented in the last decade for size-based sample preparation, owing to its high separation performances for a wide range of particle dimensions. However, separating particles from 1 μm to 10 μm in one single DLD device is challenging because of the required diversity of pillar dimensions and inherent fabrication issues. This paper presents an alternative approach to achieve the extraction of E. coli bacteria from blood samples spiked with prostate cancer cells. Our approach consists in cascading individual DLD devices in a single automated platform, using flexible chambers that successively collect and inject the sample between each DLD stage without any external sample manipulation. Operating DLD separations independently enables to maximize the sorting efficiency at each step, without any disturbance from downstream stages. The proposed two-step automated protocol is applied to the separation of three types of components (bacteria, blood particles and cancer cells), with a depletion yield of 100% for cancer cells and 93% for red blood cells. This cascaded approach is presented for the first time with two DLD modules and is upscalable to improve the dynamic range of currently available DLD devices., This work was supported by a CFR CEA grant for Ph.D. funding, by the FET Proactive H2020 Viruscan project (No. 731868) and by the BactiDIAG grant funded by BPI France under the FUI framework (FUI-AAP18).

Published
2018

5. Integration of a dilution range using collapsible chambers in a X-Y architecture

Author

Parent, Charlotte, Boizot, François, Cubizolles, Myriam, Verplanck, Nicolas, Achard, Jean-Luc, Fouillet, Yves, Département Microtechnologies pour la Biologie et la Santé (DTBS), Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Service Bio-System on Chip (SBSC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), Laboratoire des Écoulements Géophysiques et Industriels [Grenoble] (LEGI ), Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), and Ghigliotti, Giovanni

Subjects
[PHYS.MECA.MEFL] Physics [physics]/Mechanics [physics]/Fluid mechanics [physics.class-ph], [PHYS.MECA.MEFL]Physics [physics]/Mechanics [physics]/Fluid mechanics [physics.class-ph], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2017

11. Purification of complex samples: Implementation of a modular and reconfigurable droplet-based microfluidic platform with cascaded deterministic lateral displacement separation modules

Author

Pariset, Eloise, Pudda, Catherine, Boizot, François, Verplanck, Nicolas, Revol-Cavalier, Frédéric, Berthier, Jean, Thuaire, Aurélie, Agache, Vincent, European Commission, Bpifrance, Pariset, Eloise, and Pariset, Eloise [0000-0002-9375-2361]

Subjects
Imaging Techniques, Materials by Structure, Microfluidics, Materials Science, lcsh:Medicine, Fluid Mechanics, Aqueous Solutions, Research and Analysis Methods, Biochemistry, Continuum Mechanics, Mathematical and Statistical Techniques, Lab-On-A-Chip Devices, Fluorescence Imaging, Nanotechnology, Statistical Methods, lcsh:Science, Flow Rate, Physics, lcsh:R, Biology and Life Sciences, Classical Mechanics, Fluid Dynamics, Microfluidic Analytical Techniques, Lipids, Solutions, Microscopy, Fluorescence, Mixtures, Physical Sciences, Engineering and Technology, Nanoparticles, lcsh:Q, Fluidics, Oils, Mathematics, Statistics (Mathematics), Research Article, Forecasting
Abstract

Particle separation in microfluidic devices is a common problematic for sample preparation in biology. Deterministic lateral displacement (DLD) is efficiently implemented as a sizebased fractionation technique to separate two populations of particles around a specific size. However, real biological samples contain components of many different sizes and a single DLD separation step is not sufficient to purify these complex samples. When connecting several DLD modules in series, pressure balancing at the DLD outlets of each step becomes critical to ensure an optimal separation efficiency. A generic microfluidic platform is presented in this paper to optimize pressure balancing, when DLD separation is connected either to another DLD module or to a different microfluidic function. This is made possible by generating droplets at T-junctions connected to the DLD outlets. Droplets act as pressure controllers, which perform at the same time the encapsulation of DLD sorted particles and the balance of output pressures. The optimized pressures to apply on DLD modules and on T-junctions are determined by a general model that ensures the equilibrium of the entire platform. The proposed separation platform is completely modular and reconfigurable since the same predictive model applies to any cascaded DLD modules of the dropletbased cartridge., This work was supported by a CFR CEA grant for Ph.D. funding, FET Proactive VIRUSCAN H2020 project (No. 731868) and BactiDIAG grant funded by BPI France under the FUI framework (FUI-AAP18).

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12. Isolation of Extracellular Vesicles from Cell Culture and Blood Through Nano-Targeted DLD Microfluidic Device.

Author

Gaillard M, Sarrut-Rio N, Pudda C, Boizot F, Roupioz Y, and Thuaire A

Subjects
Humans, Microfluidic Analytical Techniques instrumentation, Microfluidic Analytical Techniques methods, Cell Culture Techniques methods, Ultracentrifugation methods, Extracellular Vesicles metabolism, Extracellular Vesicles chemistry, Lab-On-A-Chip Devices
Abstract

Extracellular vesicles (EVs) are secreted by cells and found in biological fluids such as blood, with concentration correlated with oncogenic signals, making them attractive biomarkers for liquid biopsy. The current gold-standard method for EVs isolation requires an ultracentrifugation (UC) step among others. The cost and complexity of this technique are forbiddingly high for many researchers, as well as for routine use in biological laboratories and hospitals. This chapter reports on a simple microfluidic method for EVs isolation, based on a microfluidic size sorting technique named Deterministic Lateral Displacement (DLD). With the design of micrometric DLD array, we demonstrated the potential of our DLD devices for the isolation of nano-biological objects such as EVs, with main population size distribution consistent with UC technique., (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)

Published
2024
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