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37 results on '"Boisset, Jean-Charles"'

1. Strand-specific single-cell methylomics reveals distinct modes of DNA demethylation dynamics during early mammalian development

Author

Sen, Maya, Mooijman, Dylan, Chialastri, Alex, Boisset, Jean-Charles, Popovic, Mina, Heindryckx, Björn, Chuva de Sousa Lopes, Susana M, Dey, Siddharth S, and van Oudenaarden, Alexander

Subjects
Biochemistry and Cell Biology, Biomedical and Clinical Sciences, Biological Sciences, Genetics, Reproductive Medicine, Human Genome, Stem Cell Research, Stem Cell Research - Embryonic - Non-Human, Pediatric, 1.1 Normal biological development and functioning, Generic health relevance, Animals, Blastocyst, DNA (Cytosine-5-)-Methyltransferase 1, DNA Demethylation, DNA Methylation, Embryonic Development, Embryonic Stem Cells, Female, Humans, Mice, Mice, Knockout, Pregnancy
Abstract

DNA methylation (5mC) is central to cellular identity. The global erasure of 5mC from the parental genomes during preimplantation mammalian development is critical to reset the methylome of gametes to the cells in the blastocyst. While active and passive modes of demethylation have both been suggested to play a role in this process, the relative contribution of these two mechanisms to 5mC erasure remains unclear. Here, we report a single-cell method (scMspJI-seq) that enables strand-specific quantification of 5mC, allowing us to systematically probe the dynamics of global demethylation. When applied to mouse embryonic stem cells, we identified substantial cell-to-cell strand-specific 5mC heterogeneity, with a small group of cells displaying asymmetric levels of 5mCpG between the two DNA strands of a chromosome suggesting loss of maintenance methylation. Next, in preimplantation mouse embryos, we discovered that methylation maintenance is active till the 16-cell stage followed by passive demethylation in a fraction of cells within the early blastocyst at the 32-cell stage of development. Finally, human preimplantation embryos qualitatively show temporally delayed yet similar demethylation dynamics as mouse embryos. Collectively, these results demonstrate that scMspJI-seq is a sensitive and cost-effective method to map the strand-specific genome-wide patterns of 5mC in single cells.

Published
2021

6. Strand-specific single-cell methylomics reveals distinct modes of DNA demethylation dynamics during early mammalian development

Author

CMM Sectie Molecular Cancer Research, Cancer, Hubrecht Institute with UMC, Sen, Maya, Mooijman, Dylan, Chialastri, Alex, Boisset, Jean Charles, Popovic, Mina, Heindryckx, Björn, Chuva de Sousa Lopes, Susana M., Dey, Siddharth S., van Oudenaarden, Alexander, CMM Sectie Molecular Cancer Research, Cancer, Hubrecht Institute with UMC, Sen, Maya, Mooijman, Dylan, Chialastri, Alex, Boisset, Jean Charles, Popovic, Mina, Heindryckx, Björn, Chuva de Sousa Lopes, Susana M., Dey, Siddharth S., and van Oudenaarden, Alexander

Published
2021

7. In vivo imaging of haematopoietic cells emerging from the mouse aortic endothelium

Author

Boisset, Jean-Charles, van Cappellen, Wiggert, Andrieu-Soler, Charlotte, Galjart, Niels, Dzierzak, Elaine, and Robin, Catherine

Subjects
Endothelium -- Physiological aspects -- Research, Hematopoietic stem cells -- Physiological aspects -- Research, Diagnostic imaging -- Usage, Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

Haematopoietic stem cells (HSCs), responsible for blood production in the adult mouse, are first detected in the dorsal aorta starting at embryonic day 10.5 (E10.5) (1-3). Immunohistological analysis of fixed embryo sections has revealed the presence of haematopoietic cell clusters attached to the aortic endothelium where HSCs might localize (4-6). The origin of HSCs has long been controversial and several candidates of the direct HSC precursors have been proposed (for review see ref. 7), including a specialized endothelial cell population with a haemogenic potential. Such cells have been described both in vitro in the embryonic stem cell (ESC) culture system (8,9) and retrospectively in vivo by endothelial lineage tracing (5,10) and conditional deletion experiments (11). Whether the transition from haemogenic endothelium to HSC actually occurs in the mouse embryonic aorta is still unclear and requires direct and real-time in vivo observation. To address this issue we used time-lapse confocal imaging and a new dissection procedure to visualize the deeply located aorta. Here we show the dynamic de novo emergence of phenotypically defined HSCs ([Sca1.sup.+], [c-kit.sup.+], [CD41.sup.+]) directly from ventral aortic haemogenic endothelial cells., Live imaging technology and confocal microscopy are powerful tools to observe the development of cells in real-time and in their native and intact environment. The transparency of zebrafish embryos has [...]

Published
2010
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8. 10 young leaders

Author

Boisset, Jean-Charles

Subjects
Wineries -- Management, Wine industry -- Management, Vintners -- Management, Company business management, Business, Food and beverage industries
Abstract

Descriptions of ten of the most promising young potential leaders and entrepreneurs are listed. The young generation is bound to make waves, even in a winegrowing country as steeped in tradition as France.

Published
2003

10. Mapping the physical network of cellular interactions

Author

Boisset, Jean Charles, Vivié, Judith, Grün, Dominic, Muraro, Mauro J., Lyubimova, Anna, Van Oudenaarden, Alexander, Boisset, Jean Charles, Vivié, Judith, Grün, Dominic, Muraro, Mauro J., Lyubimova, Anna, and Van Oudenaarden, Alexander

Published
2018

11. Single-cell transcriptomics reveal the dynamic of haematopoietic stem cell production in the aorta

Author

Baron, Chloé S., Kester, Lennart, Klaus, Anna, Boisset, Jean Charles, Thambyrajah, Roshana, Yvernogeau, Laurent, Kouskoff, Valérie, Lacaud, Georges, Van Oudenaarden, Alexander, Robin, Catherine, Baron, Chloé S., Kester, Lennart, Klaus, Anna, Boisset, Jean Charles, Thambyrajah, Roshana, Yvernogeau, Laurent, Kouskoff, Valérie, Lacaud, Georges, Van Oudenaarden, Alexander, and Robin, Catherine

Published
2018

12. Blastocyst-like structures generated solely from stem cells

Author

Sub Developmental Biology, dCSCA RMSC-1, Onderzoek, Rivron, Nicolas C., Frias-Aldeguer, Javier, Vrij, Erik J., Boisset, Jean Charles, Korving, Jeroen, Vivié, Judith, Truckenmüller, Roman K., Van Oudenaarden, Alexander, Van Blitterswijk, Clemens A., Geijsen, Niels, Sub Developmental Biology, dCSCA RMSC-1, Onderzoek, Rivron, Nicolas C., Frias-Aldeguer, Javier, Vrij, Erik J., Boisset, Jean Charles, Korving, Jeroen, Vivié, Judith, Truckenmüller, Roman K., Van Oudenaarden, Alexander, Van Blitterswijk, Clemens A., and Geijsen, Niels

Published
2018

13. Single-cell transcriptomics reveal the dynamic of haematopoietic stem cell production in the aorta

Author

Baron, Chloé S, Kester, Lennart, Klaus, Anna, Boisset, Jean-Charles, Thambyrajah, Roshana, Yvernogeau, Laurent, Kouskoff, Valérie, Lacaud, Georges, van Oudenaarden, Alexander, Robin, Catherine, Baron, Chloé S, Kester, Lennart, Klaus, Anna, Boisset, Jean-Charles, Thambyrajah, Roshana, Yvernogeau, Laurent, Kouskoff, Valérie, Lacaud, Georges, van Oudenaarden, Alexander, and Robin, Catherine

Abstract

Haematopoietic stem cells (HSCs) are generated from haemogenic endothelial (HE) cells via the formation of intra-aortic haematopoietic clusters (IAHCs) in vertebrate embryos. The molecular events controlling endothelial specification, endothelial-to-haematopoietic transition (EHT) and IAHC formation, as it occurs in vivo inside the aorta, are still poorly understood. To gain insight in these processes, we performed single-cell RNA-sequencing of non-HE cells, HE cells, cells undergoing EHT, IAHC cells, and whole IAHCs isolated from mouse embryo aortas. Our analysis identified the genes and transcription factor networks activated during the endothelial-to-haematopoietic switch and IAHC cell maturation toward an HSC fate. Our study provides an unprecedented complete resource to study in depth HSC generation in vivo. It will pave the way for improving HSC production in vitro to address the growing need for tailor-made HSCs to treat patients with blood-related disorders.

Published
2018

14. Blastocyst-like structures generated solely from stem cells

Author

Rivron, Nicolas C, Frias-Aldeguer, Javier, Vrij, Erik J, Boisset, Jean-Charles, Korving, Jeroen, Vivié, Judith, Truckenmüller, Roman K, van Oudenaarden, Alexander, van Blitterswijk, Clemens A, Geijsen, Niels, Rivron, Nicolas C, Frias-Aldeguer, Javier, Vrij, Erik J, Boisset, Jean-Charles, Korving, Jeroen, Vivié, Judith, Truckenmüller, Roman K, van Oudenaarden, Alexander, van Blitterswijk, Clemens A, and Geijsen, Niels

Abstract

The blastocyst (the early mammalian embryo) forms all embryonic and extra-embryonic tissues, including the placenta. It consists of a spherical thin-walled layer, known as the trophectoderm, that surrounds a fluid-filled cavity sheltering the embryonic cells 1 . From mouse blastocysts, it is possible to derive both trophoblast 2 and embryonic stem-cell lines 3 , which are in vitro analogues of the trophectoderm and embryonic compartments, respectively. Here we report that trophoblast and embryonic stem cells cooperate in vitro to form structures that morphologically and transcriptionally resemble embryonic day 3.5 blastocysts, termed blastoids. Like blastocysts, blastoids form from inductive signals that originate from the inner embryonic cells and drive the development of the outer trophectoderm. The nature and function of these signals have been largely unexplored. Genetically and physically uncoupling the embryonic and trophectoderm compartments, along with single-cell transcriptomics, reveals the extensive inventory of embryonic inductions. We specifically show that the embryonic cells maintain trophoblast proliferation and self-renewal, while fine-tuning trophoblast epithelial morphogenesis in part via a BMP4/Nodal-KLF6 axis. Although blastoids do not support the development of bona fide embryos, we demonstrate that embryonic inductions are crucial to form a trophectoderm state that robustly implants and triggers decidualization in utero. Thus, at this stage, the nascent embryo fuels trophectoderm development and implantation.

Published
2018

15. Mapping the physical network of cellular interactions

Author

CMM Sectie Molecular Cancer Research, Cancer, Hubrecht Institute with UMC, Boisset, Jean Charles, Vivié, Judith, Grün, Dominic, Muraro, Mauro J., Lyubimova, Anna, Van Oudenaarden, Alexander, CMM Sectie Molecular Cancer Research, Cancer, Hubrecht Institute with UMC, Boisset, Jean Charles, Vivié, Judith, Grün, Dominic, Muraro, Mauro J., Lyubimova, Anna, and Van Oudenaarden, Alexander

Published
2018

16. Blastocyst-like structures generated solely from stem cells

Author

CMM Sectie Molecular Cancer Research, Cancer, Hubrecht Institute with UMC, Rivron, Nicolas C., Frias-Aldeguer, Javier, Vrij, Erik J., Boisset, Jean Charles, Korving, Jeroen, Vivié, Judith, Truckenmüller, Roman K., Van Oudenaarden, Alexander, Van Blitterswijk, Clemens A., Geijsen, Niels, CMM Sectie Molecular Cancer Research, Cancer, Hubrecht Institute with UMC, Rivron, Nicolas C., Frias-Aldeguer, Javier, Vrij, Erik J., Boisset, Jean Charles, Korving, Jeroen, Vivié, Judith, Truckenmüller, Roman K., Van Oudenaarden, Alexander, Van Blitterswijk, Clemens A., and Geijsen, Niels

Published
2018

19. De Novo Prediction of Stem Cell Identity using Single-Cell Transcriptome Data

Author

Grün, Dominic, Muraro, Mauro J., Boisset, Jean Charles, Wiebrands, Kay, Lyubimova, Anna, Dharmadhikari, Gitanjali, van den Born, Maaike, van Es, Johan, Jansen, Erik, Clevers, Hans, de Koning, Eelco J.P., van Oudenaarden, Alexander, Grün, Dominic, Muraro, Mauro J., Boisset, Jean Charles, Wiebrands, Kay, Lyubimova, Anna, Dharmadhikari, Gitanjali, van den Born, Maaike, van Es, Johan, Jansen, Erik, Clevers, Hans, de Koning, Eelco J.P., and van Oudenaarden, Alexander

Published
2016

21. Single-cell 5hmC sequencing reveals chromosome-wide cell-to-cell variability and enables lineage reconstruction

Author

Hubrecht Institute with UMC, CMM Sectie Molecular Cancer Research, Cancer, Mooijman, Dylan, Dey, Siddharth S., Boisset, Jean Charles, Crosetto, Nicola, Van Oudenaarden, Alexander, Hubrecht Institute with UMC, CMM Sectie Molecular Cancer Research, Cancer, Mooijman, Dylan, Dey, Siddharth S., Boisset, Jean Charles, Crosetto, Nicola, and Van Oudenaarden, Alexander

Published
2016

22. De Novo Prediction of Stem Cell Identity using Single-Cell Transcriptome Data

Author

Hubrecht Institute with UMC, CMM Sectie Molecular Cancer Research, Regenerative Medicine and Stem Cells, Child Health, Cancer, Grün, Dominic, Muraro, Mauro J., Boisset, Jean Charles, Wiebrands, Kay, Lyubimova, Anna, Dharmadhikari, Gitanjali, van den Born, Maaike, van Es, Johan, Jansen, Erik, Clevers, Hans, de Koning, Eelco J.P., van Oudenaarden, Alexander, Hubrecht Institute with UMC, CMM Sectie Molecular Cancer Research, Regenerative Medicine and Stem Cells, Child Health, Cancer, Grün, Dominic, Muraro, Mauro J., Boisset, Jean Charles, Wiebrands, Kay, Lyubimova, Anna, Dharmadhikari, Gitanjali, van den Born, Maaike, van Es, Johan, Jansen, Erik, Clevers, Hans, de Koning, Eelco J.P., and van Oudenaarden, Alexander

Published
2016

23. De Novo Prediction of Stem Cell Identity using Single-Cell Transcriptome Data

Author

Grün, Dominic, primary, Muraro, Mauro J., additional, Boisset, Jean-Charles, additional, Wiebrands, Kay, additional, Lyubimova, Anna, additional, Dharmadhikari, Gitanjali, additional, van den Born, Maaike, additional, van Es, Johan, additional, Jansen, Erik, additional, Clevers, Hans, additional, de Koning, Eelco J.P., additional, and van Oudenaarden, Alexander, additional

Published
2016
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24. Progressive maturation toward hematopoietic stem cells in the mouse embryo aorta

Author

CMM Groep Coffer, Regenerative Medicine and Stem Cells, Infection & Immunity, Boisset, Jean-Charles, Clapes, Thomas, Klaus, Anna, Papazian, Natalie, Onderwater, Jos, Mommaas-Kienhuis, Mieke, Cupedo, Tom, Robin, Catherine, CMM Groep Coffer, Regenerative Medicine and Stem Cells, Infection & Immunity, Boisset, Jean-Charles, Clapes, Thomas, Klaus, Anna, Papazian, Natalie, Onderwater, Jos, Mommaas-Kienhuis, Mieke, Cupedo, Tom, and Robin, Catherine

Published
2015

28. The Microtubule Plus-End Tracking Protein CLASP2 Is Required for Hematopoiesis and Hematopoietic Stem Cell Maintenance

Author

Drabek, Ksenija, primary, Gutiérrez, Laura, additional, Vermeij, Marcel, additional, Clapes, Thomas, additional, Patel, Sunita R., additional, Boisset, Jean-Charles, additional, van Haren, Jeffrey, additional, Pereira, Ana L., additional, Liu, Zhe, additional, Akinci, Umut, additional, Nikolic, Tatjana, additional, van IJcken, Wilfred, additional, van den Hout, Mirjam, additional, Meinders, Marjolein, additional, Melo, Clara, additional, Sambade, Clara, additional, Drabek, Dubravka, additional, Hendriks, Rudi W., additional, Philipsen, Sjaak, additional, Mommaas, Mieke, additional, Grosveld, Frank, additional, Maiato, Helder, additional, Italiano, Joseph E., additional, Robin, Catherine, additional, and Galjart, Niels, additional

Published
2012
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32. Setting the trend in the wine world

Author

Boisset, Jean Charles

Subjects
Wine industry -- Environmental aspects, Wine industry -- Licensing, certification and accreditation, Wineries -- Environmental aspects, Wineries -- Licensing, certification and accreditation, Business, Food and beverage industries
Abstract

Company: Boisset Family Estates Location: Nuits-St-Georges, France Products: A portfolio of wines for every moment, mode of consumption and occasion Why an Eco Leader? Boisset Family Estate's estate vineyards, in [...]

Published
2008

36. Single-cell 5hmC sequencing reveals chromosome-wide cell-to-cell variability and enables lineage reconstruction.

Author

Mooijman D, Dey SS, Boisset JC, Crosetto N, and van Oudenaarden A

Subjects
5-Methylcytosine chemistry, Animals, Cell Differentiation genetics, Chromosome Mapping methods, Computer Simulation, Epigenesis, Genetic genetics, Genetic Variation genetics, Male, Mice, Models, Chemical, Models, Genetic, 5-Methylcytosine analogs & derivatives, Cell Lineage genetics, Chromosomes chemistry, Chromosomes genetics, Embryonic Development genetics, Sequence Analysis, DNA methods
Abstract

The epigenetic DNA modification 5-hydroxymethylcytosine (5hmC) has crucial roles in development and gene regulation. Quantifying the abundance of this epigenetic mark at the single-cell level could enable us to understand its roles. We present a single-cell, genome-wide and strand-specific 5hmC sequencing technology, based on 5hmC glucosylation and glucosylation-dependent digestion of DNA, that reveals pronounced cell-to-cell variability in the abundance of 5hmC on the two DNA strands of a given chromosome. We develop a mathematical model that reproduces the strand bias and use this model to make two predictions. First, the variation in strand bias should decrease when 5hmC turnover increases. Second, the strand bias of two sister cells should be strongly anti-correlated. We validate these predictions experimentally, and use our model to reconstruct lineages of two- and four-cell mouse embryos, showing that single-cell 5hmC sequencing can be used as a lineage reconstruction tool.

Published
2016
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37. [Endothelial origin for hematopoietic stem cells: a visual proof].

Author

Boisset JC and Robin C

Subjects
Animals, Aorta cytology, Aorta embryology, Birds embryology, Bone Marrow Transplantation, Cell Lineage, Embryo, Nonmammalian cytology, Endothelium, Vascular cytology, Endothelium, Vascular embryology, Hematopoietic Stem Cell Transplantation, Hematopoietic System embryology, Humans, Mammals embryology, Mesoderm cytology, Mice, Radiation Chimera, Species Specificity, Zebrafish embryology, Endothelial Cells cytology, Hematopoietic Stem Cells cytology, Microscopy, Confocal methods
Abstract

Hematopoietic stem cells (HSC) are the source of all blood cell types produced during the entire life of an organism. They appear during embryonic development, where they will transit through different successive hematopoietic organs, before to finally colonize the bone marrow. Nowadays, the precise origin of HSC remains a matter of controversy. Different HSC precursor candidates, located in different anatomical sites, have been proposed. Here, we summarize and discuss the different theories in light of the recent articles, especially those using in vivo confocal microscopy technology., (© 2011 médecine/sciences – Inserm / SRMS.)

Published
2011
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