108 results on '"Bohineust A"'
Search Results
2. SELECTIVE ACOUSTIC INTENSITY MEASUREMENTS OF THE RADIATION OF SURFACES SUBJECT TO SINGLE OR MULTIPLE EXCITATIONS
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WAGSTAFF, PR, primary, HENRIO, J, additional, and BOHINEUST, X, additional
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- 2024
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3. NOISE SOURCE IDENTIFICATION AND MULTIPLE SOURCE IMAGING TECHNIQUES
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WAGSTAFF, PR, primary, BOUIZEM, B, additional, BOHINEUST, X, additional, and HENRIO, JC, additional
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- 2024
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4. Azithromycin promotes relapse by disrupting immune and metabolic networks after allogeneic stem cell transplantation
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Vallet, Nicolas, Le Grand, Sophie, Bondeelle, Louise, Hoareau, Bénédicte, Corneau, Aurélien, Bouteiller, Delphine, Tournier, Simon, Derivry, Lucille, Bohineust, Armelle, Tourret, Marie, Gibert, Delphine, Mayeur, Ethan, Itzykson, Raphael, Pacchiardi, Kim, Ingram, Brian, Cassonnet, Stéphane, Lepage, Patricia, Peffault de Latour, Régis, Socié, Gérard, Bergeron, Anne, and Michonneau, David
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- 2022
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5. Integration of intermittent calcium signals in T cells revealed by temporally patterned optogenetics
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Béatrice Corre, Yassine El Janati Elidrissi, Justine Duval, Mailys Quilhot, Gaëtan Lefebvre, Solène Ecomard, Fabrice Lemaître, Zacarias Garcia, Armelle Bohineust, Erica Russo, and Philippe Bousso
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Biological sciences ,Biochemistry ,Immunology ,Immunological methods ,Science - Abstract
Summary: T cells become activated following one or multiple contacts with antigen-presenting cells. Calcium influx is a key signaling event elicited during these cellular interactions; however, it is unclear whether T cells recall and integrate calcium signals elicited during temporally separated contacts. To study the integration of calcium signals, we designed a programmable, multiplex illumination strategy for temporally patterned optogenetics (TEMPO). We found that a single round of calcium elevation was insufficient to promote nuclear factor of activated T cells (NFAT) activity and cytokine production in a T cell line. However, robust responses were detected after a second identical stimulation even when signals were separated by several hours. Our results suggest the existence of a biochemical memory of calcium signals in T cells that favors signal integration during temporally separated contacts and promote cytokine production. As illustrated here, TEMPO is a versatile approach for dissecting temporal integration in defined signaling pathways.
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- 2023
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6. Human MAIT cells inhibit alloreactive T-cell responses and protect from acute graft-versus-host disease
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Talvard-Balland, Nana, primary, Lambert, Marion, additional, Chevalier, Mathieu F., additional, Minet, Norbert, additional, Salou, Marion, additional, Tourret, Marie, additional, Bohineust, Armelle, additional, Milo, Idan, additional, Parietti, Véronique, additional, Yvorra, Thomas, additional, Socié, Gérard, additional, Lantz, Olivier, additional, and Caillat-Zucman, Sophie, additional
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- 2024
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7. Mucosal-associated invariant T (MAIT) cells, a new source of universal immune cells for chimeric antigen receptor (CAR)-cell therapy
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Bohineust, Armelle, Tourret, Marie, Derivry, Lucille, and Caillat-Zucman, Sophie
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- 2021
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8. Supplementary Table 3 from SUV39H1 Ablation Enhances Long-term CAR T Function in Solid Tumors
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López-Cobo, Sheila, primary, Fuentealba, Jaime R., primary, Gueguen, Paul, primary, Bonté, Pierre-Emmanuel, primary, Tsalkitzi, Kyriaki, primary, Chacón, Irena, primary, Glauzy, Salomé, primary, Bohineust, Armelle, primary, Biquand, Ariane, primary, Silva, Lisseth, primary, Gouveia, Zelia, primary, Goudot, Christel, primary, Perez, Franck, primary, Saitakis, Michael, primary, and Amigorena, Sebastian, primary
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- 2024
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9. SUV39H1 Ablation Enhances Long-Term CAR-T Function in Solid Tumors
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Lopez-Cobo, Sheila, primary, Fuentealba, Jaime R., additional, Gueguen, Paul, additional, Bonte, Pierre-Emmanuel, additional, Tsalkitzi, Kyriaki, additional, Chacon, Irena, additional, Glauzy, Salome, additional, Bohineust, Armelle, additional, Biquand, Ariane, additional, Silva, Lisseth, additional, Gouveia, Zelia, additional, Goudot, Christel, additional, Perez, Franck, additional, Saitakis, Michael, additional, and Amigorena, Sebastian, additional
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- 2023
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10. Optogenetic manipulation of calcium signals in single T cells in vivo
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Armelle Bohineust, Zacarias Garcia, Béatrice Corre, Fabrice Lemaître, and Philippe Bousso
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Science - Abstract
The ability to manipulate and monitor calcium signaling in cells in vivo would provide insights into signaling in an endogenous context. Here the authors develop a two-photon-responsive calcium actuator and reporter combination to monitor the effect of calcium actuation on T cell migration, adhesion and chemokine release in vivo.
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- 2020
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11. Azithromycin promotes relapse by disrupting immune and metabolic networks after allogeneic stem cell transplantation
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Nicolas Vallet, Sophie Le Grand, Louise Bondeelle, Bénédicte Hoareau, Aurélien Corneau, Delphine Bouteiller, Simon Tournier, Lucille Derivry, Armelle Bohineust, Marie Tourret, Delphine Gibert, Ethan Mayeur, Raphael Itzykson, Kim Pacchiardi, Brian Ingram, Stéphane Cassonnet, Patricia Lepage, Régis Peffault de Latour, Gérard Socié, Anne Bergeron, and David Michonneau
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Neoplasms ,Immunology ,Hematopoietic Stem Cell Transplantation ,Humans ,Cell Biology ,Hematology ,Azithromycin ,Biochemistry ,Metabolic Networks and Pathways ,Stem Cell Transplantation - Abstract
Administration of azithromycin after allogeneic hematopoietic stem cell transplantation for hematologic malignancies has been associated with relapse in a randomized phase 3 controlled clinical trial. Studying 240 samples from patients randomized in this trial is a unique opportunity to better understand the mechanisms underlying relapse, the first cause of mortality after transplantation. We used multi-omics on patients’ samples to decipher immune alterations associated with azithromycin intake and post-transplantation relapsed malignancies. Azithromycin was associated with a network of altered energy metabolism pathways and immune subsets, including T cells biased toward immunomodulatory and exhausted profiles. In vitro, azithromycin exposure inhibited T-cell cytotoxicity against tumor cells and impaired T-cell metabolism through glycolysis inhibition, down-regulation of mitochondrial genes, and up-regulation of immunomodulatory genes, notably SOCS1. These results highlight that azithromycin directly affects immune cells that favor relapse, which raises caution about long-term use of azithromycin treatment in patients at high risk of malignancies. The ALLOZITHRO trial was registered at www.clinicaltrials.gov as #NCT01959100.
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- 2022
12. Optogenetic manipulation of calcium signals in single T cells in vivo
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Bohineust, Armelle, Garcia, Zacarias, Corre, Béatrice, Lemaître, Fabrice, and Bousso, Philippe
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- 2020
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13. X-linked primary immunodeficiency associated with hemizygous mutations in the moesin (MSN) gene
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Lagresle-Peyrou, Chantal, Luce, Sonia, Ouchani, Farid, Soheili, Tayebeh Shabi, Sadek, Hanem, Chouteau, Myriam, Durand, Amandine, Pic, Isabelle, Majewski, Jacek, Brouzes, Chantal, Lambert, Nathalie, Bohineust, Armelle, Verhoeyen, Els, Cosset, François-Loïc, Magerus-Chatinet, Aude, Rieux-Laucat, Frédéric, Gandemer, Virginie, Monnier, Delphine, Heijmans, Catherine, van Gijn, Marielle, Dalm, Virgil A., Mahlaoui, Nizar, Stephan, Jean-Louis, Picard, Capucine, Durandy, Anne, Kracker, Sven, Hivroz, Claire, Jabado, Nada, de Saint Basile, Geneviève, Fischer, Alain, Cavazzana, Marina, and André-Schmutz, Isabelle
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- 2016
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14. Human MAIT cells are devoid of alloreactive potential: prompting their use as universal cells for adoptive immune therapy
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Tourret, Marie, Talvard-Balland, Nana, Lambert, Marion, Ben Youssef, Ghada, Chevalier, Mathieu F, Bohineust, Armelle, Yvorra, Thomas, Morin, Florence, Azarnoush, Saba, Lantz, Olivier, Dalle, Jean-Hugues, and Caillat-Zucman, Sophie
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Adult ,Male ,Immune Cell Therapies and Immune Cell Engineering ,Adolescent ,Infant, Newborn ,Infant ,Adaptive Immunity ,Middle Aged ,adoptive ,Mucosal-Associated Invariant T Cells ,Young Adult ,Child, Preschool ,Humans ,Female ,Immunotherapy ,transplantation immunology ,Child ,Aged - Abstract
Background Mucosal-associated invariant T (MAIT) cells are semi-invariant T cells that recognize microbial antigens presented by the highly conserved MR1 molecule. MAIT cells are predominantly localized in the liver and barrier tissues and are potent effectors of antimicrobial defense. MAIT cells are very few at birth and accumulate gradually over a period of about 6 years during the infancy. The cytotoxic potential of MAIT cells, as well as their newly described regulatory and tissue repair functions, open the possibility of exploiting their properties in adoptive therapy. A prerequisite for their use as ‘universal’ cells would be a lack of alloreactive potential, which remains to be demonstrated. Methods We used ex vivo, in vitro and in vivo models to determine if human MAIT cells contribute to allogeneic responses. Results We show that recovery of MAIT cells after allogeneic hematopoietic stem cell transplantation recapitulates their slow physiological expansion in early childhood, independent of recovery of non-MAIT T cells. In vitro, signals provided by allogeneic cells and cytokines do not induce sustained MAIT cell proliferation. In vivo, human MAIT cells do not expand nor accumulate in tissues in a model of T-cell-mediated xenogeneic graft-versus-host disease in immunodeficient mice. Conclusions Altogether, these results provide evidence that MAIT cells are devoid of alloreactive potential and pave the way for harnessing their translational potential in universal adoptive therapy overcoming barriers of HLA disparity. Trial registration number ClinicalTrials.gov number NCT02403089.
- Published
- 2021
15. Mucosal-associated invariant T (MAIT) cells, a new source of universal immune cells for chimeric antigen receptor (CAR)-cell therapy
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Armelle Bohineust, Marie Tourret, Lucille Derivry, and Sophie Caillat-Zucman
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Cancer Research ,Receptors, Chimeric Antigen ,Cost-Benefit Analysis ,Antigens, CD19 ,Hematology ,General Medicine ,Mice, SCID ,Immunotherapy, Adoptive ,Proof of Concept Study ,Mucosal-Associated Invariant T Cells ,Mice ,Oncology ,Hematologic Neoplasms ,Animals ,Radiology, Nuclear Medicine and imaging - Abstract
Treatment of hematological malignancies by autologous T cells expressing a chimeric antigen receptor (CAR) is a breakthrough in the field of cancer immunotherapy. As CAR-T cells are entering advanced phases of clinical development, there is a need to develop universal, ready-to-use products using immune cells from healthy donors, to reduce time to treatment, improve response rate and finally reduce the cost of production. Mucosal-associated invariant T cells (MAIT) are unconventional T cells which recognize microbial-derived riboflavin derivatives presented by the conserved MR1 molecule and are endowed with potent effector functions. Because they are not selected by classical MHC/peptide complexes and express a semi-invariant T cell receptor, MAIT cells do not mediate alloreactivity, prompting their use as a new source of universal effector cells for allogeneic CAR-T cell therapy without the need to inactivate their endogenous TCR. We produced CD19-CAR MAIT cells as proof-of-concept allowing subsequent head-to-head comparison with currently used CD19-CAR T cells. We demonstrated their anti-tumor efficacy in vitro and their capacity to engraft without mediating GVHD in preclinical immunodeficient mouse models. Universal, off-the-shelf CAR-MAIT cells could provide a suitable alternative to current autologous CAR-T cells to treat patients regardless of HLA disparity, without production delay, enabling a cost-effective manufacturing model for large-scale clinical application.
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- 2021
16. Human MAIT cells are devoid of alloreactive potential: prompting their use as universal cells for adoptive immune therapy
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Saba Azarnoush, Sophie Caillat-Zucman, Florence Morin, Jean Hugues Dalle, Olivier Lantz, N. Talvard-Balland, Thomas Yvorra, Mélanie Lambert, G. Ben Youssef, Armelle Bohineust, M. F. Chevalier, and M. Tourret
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Antigen ,In vivo ,Cell growth ,medicine.medical_treatment ,Immunology ,medicine ,Cytotoxic T cell ,Human leukocyte antigen ,Hematopoietic stem cell transplantation ,Biology ,Ex vivo ,In vitro - Abstract
BackgroundMucosal associated invariant T (MAIT) cells are semi-invariant T cells that recognize microbial antigens presented by the highly conserved MR1 molecule. MAIT cells are predominantly localized in the liver and barrier tissues and are potent effectors of anti - microbial defense. MAIT cells are very few at birth and accumulate gradually over a period of about 6 years during infancy. The cytotoxic potential of MAIT cells, as well as their newly described regulatory and tissue repair functions, open the possibility of exploiting their properties in adoptive therapy. A prerequisite for their use as “universal” cells would be a lack of alloreactive potential, which remains to be demonstrated.MethodsWe used ex vivo, in vitro and in vivo models to determine if human MAIT cells contribute to allogeneic responses.ResultsWe show that recovery of MAIT cells after allogeneic hematopoietic stem cell transplantation recapitulates their slow physiological expansion in early childhood, independent of recovery of conventional T cells. In vitro, signals provided by allogeneic cells and cytokines do not induce sustained MAIT cell proliferation. In vivo, human MAIT cells do not expand nor accumulate in tissues in a model of T-cell mediated xenogeneic graft-versus-host disease (GVHD) in immunodeficient mice.ConclusionsAltogether, these results provide evidence that MAIT cells are devoid of alloreactive potential and pave the way for harnessing their translational potential in universal adoptive therapy overcoming barriers of HLA disparity.
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- 2021
17. VAMP7 controls T cell activation by regulating the recruitment and phosphorylation of vesicular Lat at TCR-activation sites
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Larghi, Paola, Williamson, David J, Carpier, Jean-Marie, Dogniaux, Stéphanie, Chemin, Karine, Bohineust, Armelle, Danglot, Lydia, Gaus, Katharina, Galli, Thierry, and Hivroz, Claire
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- 2013
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18. MAIT cells detect and efficiently lyse bacterially-infected epithelial cells.
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Lionel Le Bourhis, Mathilde Dusseaux, Armelle Bohineust, Stéphanie Bessoles, Emmanuel Martin, Virginie Premel, Maxime Coré, David Sleurs, Nacer-Eddine Serriari, Emmanuel Treiner, Claire Hivroz, Philippe Sansonetti, Marie-Lise Gougeon, Claire Soudais, and Olivier Lantz
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Immunologic diseases. Allergy ,RC581-607 ,Biology (General) ,QH301-705.5 - Abstract
Mucosal associated invariant T cells (MAIT) are innate T lymphocytes that detect a large variety of bacteria and yeasts. This recognition depends on the detection of microbial compounds presented by the evolutionarily conserved major-histocompatibility-complex (MHC) class I molecule, MR1. Here we show that MAIT cells display cytotoxic activity towards MR1 overexpressing non-hematopoietic cells cocultured with bacteria. The NK receptor, CD161, highly expressed by MAIT cells, modulated the cytokine but not the cytotoxic response triggered by bacteria infected cells. MAIT cells are also activated by and kill epithelial cells expressing endogenous levels of MRI after infection with the invasive bacteria Shigella flexneri. In contrast, MAIT cells were not activated by epithelial cells infected by Salmonella enterica Typhimurium. Finally, MAIT cells are activated in human volunteers receiving an attenuated strain of Shigella dysenteriae-1 tested as a potential vaccine. Thus, in humans, MAIT cells are the most abundant T cell subset able to detect and kill bacteria infected cells.
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- 2013
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19. Human MAIT cells are devoid of alloreactive potential: prompting their use as universal cells for adoptive immune therapy
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Tourret, Marie, primary, Talvard-Balland, Nana, additional, Lambert, Marion, additional, Ben Youssef, Ghada, additional, Chevalier, Mathieu F., additional, Bohineust, Armelle, additional, Yvorra, Thomas, additional, Morin, Florence, additional, Azarnoush, Saba, additional, Lantz, Olivier, additional, Dalle, Jean-Hugues, additional, and Caillat-Zucman, Sophie, additional
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- 2021
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20. Force generation upon T cell receptor engagement.
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Julien Husson, Karine Chemin, Armelle Bohineust, Claire Hivroz, and Nelly Henry
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Medicine ,Science - Abstract
T cells are major players of adaptive immune response in mammals. Recognition of an antigenic peptide in association with the major histocompatibility complex at the surface of an antigen presenting cell (APC) is a specific and sensitive process whose mechanism is not fully understood. The potential contribution of mechanical forces in the T cell activation process is increasingly debated, although these forces are scarcely defined and hold only limited experimental evidence. In this work, we have implemented a biomembrane force probe (BFP) setup and a model APC to explore the nature and the characteristics of the mechanical forces potentially generated upon engagement of the T cell receptor (TCR) and/or lymphocyte function-associated antigen-1 (LFA-1). We show that upon contact with a model APC coated with antibodies towards TCR-CD3, after a short latency, the T cell developed a timed sequence of pushing and pulling forces against its target. These processes were defined by their initial constant growth velocity and loading rate (force increase per unit of time). LFA-1 engagement together with TCR-CD3 reduced the growing speed during the pushing phase without triggering the same mechanical behavior when engaged alone. Intracellular Ca(2+) concentration ([Ca(2+)](i)) was monitored simultaneously to verify the cell commitment in the activation process. [Ca(2+)](i) increased a few tens of seconds after the beginning of the pushing phase although no strong correlation appeared between the two events. The pushing phase was driven by actin polymerization. Tuning the BFP mechanical properties, we could show that the loading rate during the pulling phase increased with the target stiffness. This indicated that a mechanosensing mechanism is implemented in the early steps of the activation process. We provide here the first quantified description of force generation sequence upon local bidimensional engagement of TCR-CD3 and discuss its potential role in a T cell mechanically-regulated activation process.
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- 2011
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21. Human Inflammatory Dendritic Cells Induce Th17 Cell Differentiation
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Segura, Elodie, Touzot, Maxime, Bohineust, Armelle, Cappuccio, Antonio, Chiocchia, Gilles, Hosmalin, Anne, Dalod, Marc, Soumelis, Vassili, and Amigorena, Sebastian
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- 2013
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22. Cytokine secretion by CD4+ T cells at the immunological synapse requires Cdc42-dependent local actin remodeling but not MTOC polarity: W25.002
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Bohineust, A., Chemin, K., Dogniaux, S., Tourret, M., Guégan, S., Miro, F., and Hivroz, C.
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- 2012
23. T-lymphocyte passive deformation is controlled by unfolding of membrane surface reservoirs
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Armelle Bohineust, Julien Husson, Lionel Guillou, Stéphanie Dogniaux, Claire Hivroz, Avin Babataheri, Michael Saitakis, and Abdul I. Barakat
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0301 basic medicine ,Membranes ,Microvilli ,T-Lymphocytes ,Cell Membrane ,Articles ,Cell Biology ,T lymphocyte ,Biology ,Deformation (meteorology) ,Exocytosis ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Membrane ,Membrane Trafficking ,Cell Movement ,Immunology ,Microscopy, Electron, Scanning ,Biophysics ,Humans ,Membrane surface ,Cell Shape ,Molecular Biology ,030217 neurology & neurosurgery - Abstract
T-lymphocyte passive deformation when squeezing through narrow capillaries is limited by the excess membrane contained in microvilli and membrane folds. During active processes such as transendothelial migration, larger deformations are made possible by an increase in membrane area, possibly through recruitment of internal membrane reservoirs., T-lymphocytes in the human body routinely undergo large deformations, both passively, when going through narrow capillaries, and actively, when transmigrating across endothelial cells or squeezing through tissue. We investigate physical factors that enable and limit such deformations and explore how passive and active deformations may differ. Employing micropipette aspiration to mimic squeezing through narrow capillaries, we find that T-lymphocytes maintain a constant volume while they increase their apparent membrane surface area upon aspiration. Human resting T-lymphocytes, T-lymphoblasts, and the leukemic Jurkat T-cells all exhibit membrane rupture above a critical membrane area expansion that is independent of either micropipette size or aspiration pressure. The unfolded membrane matches the excess membrane contained in microvilli and membrane folds, as determined using scanning electron microscopy. In contrast, during transendothelial migration, a form of active deformation, we find that the membrane surface exceeds by a factor of two the amount of membrane stored in microvilli and folds. These results suggest that internal membrane reservoirs need to be recruited, possibly through exocytosis, for large active deformations to occur.
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- 2016
24. INVESTIGATION AND MODELLING OF THE WALL PRESSURE FIELD BENEATH A TURBULENT BOUNDARY LAYER AT LOW AND MEDIUM FREQUENCIES
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LECLERCQ, D.J.J and BOHINEUST, X
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- 2002
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25. Termination of T cell priming relies on a phase of unresponsiveness promoting disengagement from APCs and T cell division
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Bohineust, Armelle, primary, Garcia, Zacarias, additional, Beuneu, Hélène, additional, Lemaître, Fabrice, additional, and Bousso, Philippe, additional
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- 2018
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26. Cytokine Secretion by CD4+ T Cells at the Immunological Synapse Requires Cdc42-Dependent Local Actin Remodeling but Not Microtubule Organizing Center Polarity
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Stéphanie Dogniaux, Marie Tourret, Sarah Guégan, Francesc Miro, Claire Hivroz, Armelle Bohineust, and Karine Chemin
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CD4-Positive T-Lymphocytes ,Immunological Synapses ,Primary Cell Culture ,Immunology ,macromolecular substances ,Biology ,Exocytosis ,Polymerization ,Immunological synapse ,Microtubule polymerization ,Jurkat Cells ,Actin remodeling of neurons ,Humans ,Immunology and Allergy ,Secretion ,cdc42 GTP-Binding Protein ,Cytoskeleton ,Cell Line, Transformed ,Cell Polarity ,Actin remodeling ,Microtubule organizing center ,Actins ,Coculture Techniques ,Cell biology ,HEK293 Cells ,Cytokines ,Cytokine secretion ,Microtubule-Organizing Center - Abstract
Cytokine secretion by T lymphocytes plays a central role in mounting adaptive immune responses. However, little is known about how newly synthesized cytokines, once produced, are routed within T cells and about the mechanisms involved in regulating their secretions. In this study, we investigated the role of cytoskeleton remodeling at the immunological synapse (IS) in cytokine secretion. We show that a key regulator of cytoskeleton remodeling, the Rho GTPase Cdc42, controls IFN-γ secretion by primary human CD4+ T lymphocytes. Surprisingly, microtubule organizing center polarity at the IS, which does not depend on Cdc42, is not required for cytokine secretion by T lymphocytes, whereas microtubule polymerization is required. In contrast, actin remodeling at the IS, which depends on Cdc42, controls the formation of the polymerized actin ring at the IS, the dynamic concentration of IFN-γ–containing vesicles inside this ring, and the secretion of these vesicles. These results reveal a previously unidentified role of Cdc42-dependent actin remodeling in cytokine exocytosis at the IS.
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- 2012
27. Crosstalk between T Lymphocytes and Dendritic Cells
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Armelle Bohineust, Marie Tourret, Karine Chemin, and Claire Hivroz
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CD4-Positive T-Lymphocytes ,Antigen Presentation ,Immunological Synapses ,Polymers and Plastics ,Follicular dendritic cells ,Peripheral Tolerance ,Antigen presentation ,Priming (immunology) ,Peripheral tolerance ,Cell Communication ,Dendritic Cells ,Receptor Cross-Talk ,Dendritic cell ,Biology ,Lymphocyte Activation ,Cell biology ,Immunology ,Animals ,Cytokines ,Humans ,Cytotoxic T cell ,IL-2 receptor ,Antigen-presenting cell ,Cytoskeleton ,General Environmental Science - Abstract
Dendritic cells (DCs) are professional antigen-presenting cells (APCs) with the unique property of inducing priming and differentiation of naïve CD4+ and CD8+ T cells into helper and cytotoxic effectors. Their efficiency is due to their unique ability to process antigen, express costimulatory molecules, secrete cytokines, and migrate to tissues or lymphoid organs to prime T cells. DCs also play an important role in T-cell peripheral tolerance. There is ample evidence that the DC ability to present antigens is regulated by CD4+ helper T cells. Indeed, interactions between surface receptors and ligands expressed respectively by T cells and DCs, as well as T-cell-derived cytokines modify DC functions. This T-cell-induced modification of DCs has been called "education" or "licensing." This intimate crosstalk between DCs and T lymphocytes is key in establishing appropriate adaptive immune responses. It requires cognate interactions between T lymphocytes and DCs, which are organized in time and space by structures called immunological synapses. Here we discuss the particular aspects of immunological synapses formed between T cells and DCs and the role these organized interactions have in T-cell-DC crosstalk.
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- 2012
28. T Cell Polarity at the Immunological Synapse Is Required for CD154-Dependent IL-12 Secretion by Dendritic Cells
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Francesc Miro, Sarah Guégan, Armelle Bohineust, Karine Chemin, Marie Tourret, Claire Hivroz, and Stéphanie Dogniaux
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CD4-Positive T-Lymphocytes ,Immunological Synapses ,T cell ,CD40 Ligand ,Immunology ,chemical and pharmacologic phenomena ,Cell Communication ,Biology ,Immunological synapse ,Interleukin 21 ,medicine ,Humans ,Immunology and Allergy ,Cytotoxic T cell ,Antigen-presenting cell ,Cells, Cultured ,ZAP70 ,Cell Polarity ,CD28 ,hemic and immune systems ,Dendritic Cells ,Natural killer T cell ,Interleukin-12 ,Coculture Techniques ,Cell biology ,medicine.anatomical_structure - Abstract
Ag-specific interaction between T lymphocytes and dendritic cells (DCs) leads to both T cell and DC activation. CD154 (CD40 ligand)/CD40 interactions have been shown to play a major, although not exclusive, role in this functional cross-talk. Interactions between T cells and DCs are structured by an immunological synapse (IS), characterized by polarization of the T cell microtubule cytoskeleton toward the interacting DCs. Yet the role T cell polarization may play in T cell-induced DC activation is mostly unknown. In this study, we address the role of T cell polarity in CD154-dependent activation of DCs in a human model, using two different tools to block T cell polarity (i.e., a microtubule depolymerizing drug and an inhibitor of atypical protein kinase C). We show that CD154 is recruited and concentrated at the IS formed between human primary T cells and autologous DCs and that this recruitment requires T cell polarity at the IS. Moreover, we show that T cell polarization at the IS controls T cell-dependent CD154–CD40 signaling in DCs as well as CD154-dependent IL-12 secretion by DCs. This study shows that T cell polarity at the IS plays a key role in CD154/CD40-dependent cross-talk between CD4+ T cells and DCs.
- Published
- 2010
29. T-lymphocyte passive deformation is controlled by unfolding of membrane surface reservoirs
- Author
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Guillou, Lionel, primary, Babataheri, Avin, additional, Saitakis, Michael, additional, Bohineust, Armelle, additional, Dogniaux, Stéphanie, additional, Hivroz, Claire, additional, Barakat, Abdul I., additional, and Husson, Julien, additional
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- 2016
- Full Text
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30. X-linked primary immunodeficiency associated with hemizygous mutations in the moesin (MSN) gene
- Author
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Genetica Sectie Genoomdiagnostiek, Infection & Immunity, Lagresle-Peyrou, Chantal, Luce, Sonia, Ouchani, Farid, Soheili, Tayebeh Shabi, Sadek, Hanem, Chouteau, Myriam, Durand, Amandine, Pic, Isabelle, Majewski, Jacek, Brouzes, Chantal, Lambert, Nathalie, Bohineust, Armelle, Verhoeyen, Els, Cosset, François-Loïc, Magerus-Chatinet, Aude, Rieux-Laucat, Frédéric, Gandemer, Virginie, Monnier, Delphine, Heijmans, Catherine, van Gijn, Marielle, Dalm, Virgil A, Mahlaoui, Nizar, Stephan, Jean-Louis, Picard, Capucine, Durandy, Anne, Kracker, Sven, Hivroz, Claire, Jabado, Nada, de Saint Basile, Geneviève, Fischer, Alain, Cavazzana, Marina, André-Schmutz, Isabelle, Genetica Sectie Genoomdiagnostiek, Infection & Immunity, Lagresle-Peyrou, Chantal, Luce, Sonia, Ouchani, Farid, Soheili, Tayebeh Shabi, Sadek, Hanem, Chouteau, Myriam, Durand, Amandine, Pic, Isabelle, Majewski, Jacek, Brouzes, Chantal, Lambert, Nathalie, Bohineust, Armelle, Verhoeyen, Els, Cosset, François-Loïc, Magerus-Chatinet, Aude, Rieux-Laucat, Frédéric, Gandemer, Virginie, Monnier, Delphine, Heijmans, Catherine, van Gijn, Marielle, Dalm, Virgil A, Mahlaoui, Nizar, Stephan, Jean-Louis, Picard, Capucine, Durandy, Anne, Kracker, Sven, Hivroz, Claire, Jabado, Nada, de Saint Basile, Geneviève, Fischer, Alain, Cavazzana, Marina, and André-Schmutz, Isabelle
- Published
- 2016
31. Analysis of Medium-Frequency Vibrations in a Frequency Range
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Philippe Rouch, X. Bohineust, Hervé Riou, and Pierre Ladevèze
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Vibration ,Range (mathematics) ,Acoustics and Ultrasonics ,Applied Mathematics ,Acoustics ,Mathematical analysis ,Emphasis (telecommunications) ,Structure (category theory) ,Medium frequency ,Mathematics - Abstract
A new approach called the ''Variational Theory of Complex Rays'' (VTCR) is being developed in order to calculate the vibrations of slightly damped elastic structures in the medium-frequency range. Here, the emphasis is put on the extension of this theory to analysis across a range of frequencies. Numerical examples show the capability of the VTCR to predict the vibrational response of a structure in a frequency range.
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- 2003
32. INVESTIGATION AND MODELLING OF THE WALL PRESSURE FIELD BENEATH A TURBULENT BOUNDARY LAYER AT LOW AND MEDIUM FREQUENCIES
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X Bohineust and D.J.J. Leclercq
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Acoustics and Ultrasonics ,Field (physics) ,Anechoic chamber ,Turbulence ,Mechanical Engineering ,Computation ,Flow (psychology) ,Mechanics ,Condensed Matter Physics ,Coherence length ,Physics::Fluid Dynamics ,Boundary layer ,Mechanics of Materials ,Phase velocity ,Simulation ,Geology - Abstract
In the case of a vehicle moving in a fluid, computation of flow-induced panel vibrations, and the resulting sound generated inside the passenger compartment requires a model that describes the statistics of the turbulent wall pressure fluctuations accurately. However, the models currently available in the literature usually rely on simplifying assumptions and necessitate specific measurements in order to, for example, evaluate the evolution of the coherence length with frequency. This paper describes the work done in order to propose a semi-empirical model for the wall pressure field beneath a fully turbulent boundary layer flow. The main goal is to try to avoid the necessity of preliminary experiments to determine the model coefficients, i.e., propose a model for the power spectral density, coherence length, and phase velocity. After a quick literature review, it proves necessary to acquire a set of experimental data with a fine space–time resolution. Fluctuating wall pressure transducers are developed in this perspective, and experiments are performed in an anechoic wind testing facility. The results are then compared with some published data, analyzed to attempt to offer a schematic description of the physical phenomena involved, and to define the pertinent parameters of the flow and properties of the wall pressure field. These observations are then used to propose a new model for the wall pressure field beneath a fully turbulent boundary layer flow.
- Published
- 2002
33. X-linked primary immunodeficiency associated with hemizygous mutations in the moesin (MSN) gene
- Author
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Jacek Majewski, Marielle E. van Gijn, Alain Fischer, Geneviève de Saint Basile, Capucine Picard, Nada Jabado, Els Verhoeyen, Sonia Luce, Tayebeh Soheili, Nathalie Lambert, Amandine Durand, Armelle Bohineust, Claire Hivroz, Virginie Gandemer, Farid Ouchani, Chantal Brouzes, Catherine Heijmans, Anne Durandy, Isabelle Pic, Myriam Chouteau, Marina Cavazzana, Virgil A. S. H. Dalm, Nizar Mahlaoui, Aude Magerus-Chatinet, François-Loïc Cosset, Isabelle André-Schmutz, Delphine Monnier, Sven Kracker, Chantal Lagresle-Peyrou, Jean-Louis Stephan, Frédéric Rieux-Laucat, Hanem Sadek, Human Lymphohematopoiesis Laboratory (Equipe Inserm U1163), Imagine - Institut des maladies génétiques (IMAGINE - U1163), Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Département de Biothérapie [CHU Necker], CHU Necker - Enfants Malades [AP-HP]-Université Paris Descartes - Paris 5 (UPD5)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP), McGill University and Genome Quebec Innovation Centre, CHU Necker - Enfants Malades [AP-HP], Center for the Study of Primary Immunodeficiencies [Paris], Université Paris Descartes - Paris 5 (UPD5)-CHU Necker - Enfants Malades [AP-HP], Immunité et cancer (U932), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Curie-Université Paris Descartes - Paris 5 (UPD5), Centre méditerranéen de médecine moléculaire (C3M), Université Nice Sophia Antipolis (... - 2019) (UNS), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Virus enveloppés, vecteurs et immunothérapie – Enveloped viruses, Vectors and Immuno-therapy (Equipe EVIR), Centre International de Recherche en Infectiologie - UMR (CIRI), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Immunogenetics of pediatric autoimmune diseases (Equipe Inserm U1163), Institut de Génétique et Développement de Rennes (IGDR), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), CHU Pontchaillou [Rennes], Service d'Hématologie, Immunologie et de Thérapie Cellulaire (HITC), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Hôpital Pontchaillou-CHU Pontchaillou [Rennes], Hôpital Universitaire des Enfants - Reine Fabiola, Dpt of Genetics [Utrecht], University Medical Center [Utrecht], Department of Internal Medicine, Erasmus University Medical Center [Rotterdam] (Erasmus MC), Centre de Référence Déficits Immunitaires Héréditaires (CEREDIH), Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-CHU Necker - Enfants Malades [AP-HP], Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service d'immuno-hématologie pédiatrique [CHU Necker], CHU Saint-Etienne, the Genome Quebec Innovation Centre, McGill University, Collège de France (CdF), French National Research Agency, French National Institute of Health and Medical Research (INSERM), La Ligue contre le Cancer and La Fondation ARC pour la Recherche sur le Cancer, Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Descartes - Paris 5 (UPD5)-CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Université Paris Descartes - Paris 5 (UPD5)-Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA), Virus enveloppés, vecteurs et immunothérapie – Enveloped viruses, Vectors and Immuno-therapy (EVIR), Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Université de Rennes (UR)-Hôpital Pontchaillou-CHU Pontchaillou [Rennes], Hôpital Universitaire des Enfants Reine Fabiola [Bruxelles, Belgique] (HUDERF), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Necker - Enfants Malades [AP-HP], Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre Hospitalier Universitaire de Saint-Etienne [CHU Saint-Etienne] (CHU ST-E), McGill University = Université McGill [Montréal, Canada], Collège de France - Chaire Médecine expérimentale (A. Fischer), Collège de France (CdF (institution)), Imagine - Institut des maladies génétiques ( IMAGINE - U1163 ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), CIC-BT 502 INSERM, Departement de Biotherapie, Laboratoire d'hématologie ( ERL 8254 ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Centre de Référence Déficits Immunitaires Héréditaires ( CEREDIH ), Assistance publique - Hôpitaux de Paris (AP-HP)-CHU Necker - Enfants Malades [AP-HP], Immunité et cancer ( U932 ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut Curie-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Centre méditérannéen de médecine moléculaire ( C3M ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Université Nice Sophia Antipolis ( UNS ), Université Côte d'Azur ( UCA ) -Université Côte d'Azur ( UCA ), Centre International de Recherche en Infectiologie ( CIRI ), École normale supérieure - Lyon ( ENS Lyon ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Institut de Génétique et Développement de Rennes ( IGDR ), Université de Rennes 1 ( UR1 ), Université de Rennes ( UNIV-RENNES ) -Université de Rennes ( UNIV-RENNES ) -Centre National de la Recherche Scientifique ( CNRS ) -Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Service d'Hématologie, Immunologie et de Thérapie Cellulaire ( HITC ), Université de Rennes ( UNIV-RENNES ) -Université de Rennes ( UNIV-RENNES ) -Hôpital Pontchaillou-CHU Pontchaillou [Rennes], Collège de France ( CdF ), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Descartes - Paris 5 (UPD5), Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Chaire Médecine expérimentale (A. Fischer), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Université Paris Descartes - Paris 5 (UPD5)-CHU Necker - Enfants Malades [AP-HP], Institut Curie-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Immunology, and Internal Medicine
- Subjects
Adult ,Male ,0301 basic medicine ,Adolescent ,Moesin ,Immunology ,Nonsense mutation ,macromolecular substances ,CD8-Positive T-Lymphocytes ,Biology ,Infections ,primary immunodeficiency ,migration ,medicine.disease_cause ,ezrin-radixin-moesin protein ,Hypogammaglobulinemia ,03 medical and health sciences ,Cell Movement ,Radixin ,Cell Adhesion ,Journal Article ,medicine ,Humans ,Immunology and Allergy ,Missense mutation ,Lymphocyte Count ,Child ,moesin ,Genetic Association Studies ,Immunodeficiency ,Aged ,Chromosomes, Human, X ,[SDV.GEN]Life Sciences [q-bio]/Genetics ,Mutation ,Microfilament Proteins ,Immunologic Deficiency Syndromes ,Leukopenia ,medicine.disease ,Pedigree ,3. Good health ,adhesion ,030104 developmental biology ,Child, Preschool ,Cancer research ,Primary immunodeficiency ,[ SDV.GEN ] Life Sciences [q-bio]/Genetics - Abstract
International audience; BACKGROUND: We investigated 7 male patients (from 5 different families) presenting with profound lymphopenia, hypogammaglobulinemia, fluctuating monocytopenia and neutropenia, a poor immune response to vaccine antigens, and increased susceptibility to bacterial and varicella zoster virus infections. OBJECTIVE: We sought to characterize the genetic defect involved in a new form of X-linked immunodeficiency. METHODS: We performed genetic analyses and an exhaustive phenotypic and functional characterization of the lymphocyte compartment. RESULTS: We observed hemizygous mutations in the moesin (MSN) gene (located on the X chromosome and coding for MSN) in all 7 patients. Six of the latter had the same missense mutation, which led to an amino acid substitution (R171W) in the MSN four-point-one, ezrin, radixin, moesin domain. The seventh patient had a nonsense mutation leading to a premature stop codon mutation (R533X). The naive T-cell counts were particularly low for age, and most CD8(+) T cells expressed the senescence marker CD57. This phenotype was associated with impaired T-cell proliferation, which was rescued by expression of wild-type MSN. MSN-deficient T cells also displayed poor chemokine receptor expression, increased adhesion molecule expression, and altered migration and adhesion capacities. CONCLUSION: Our observations establish a causal link between an ezrin-radixin-moesin protein mutation and a primary immunodeficiency that could be referred to as X-linked moesin-associated immunodeficiency.
- Published
- 2016
34. VAMP7 controls T cell activation by regulating the recruitment and phosphorylation of vesicular Lat at TCR-activation sites
- Author
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Stéphanie Dogniaux, Claire Hivroz, Lydia Danglot, Jean-Marie Carpier, Karine Chemin, Katharina Gaus, Paola Larghi, Armelle Bohineust, Thierry Galli, and David Williamson
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Male ,Immunological Synapses ,T cell ,T-Lymphocytes ,Immunology ,Immunoblotting ,Receptors, Antigen, T-Cell ,Priming (immunology) ,chemical and pharmacologic phenomena ,Lymphocyte Activation ,Jurkat cells ,R-SNARE Proteins ,Jurkat Cells ,Mice ,medicine ,Immunology and Allergy ,Animals ,Humans ,Phosphorylation ,Adaptor Proteins, Signal Transducing ,Mice, Knockout ,Chemistry ,Vesicle ,T-cell receptor ,Signal transducing adaptor protein ,Membrane Proteins ,hemic and immune systems ,Flow Cytometry ,Phosphoproteins ,Research Highlight ,Cell biology ,Mice, Inbred C57BL ,medicine.anatomical_structure ,Microscopy, Fluorescence ,Signal transduction ,Signal Transduction - Abstract
The mechanisms by which Lat (a key adaptor in the T cell antigen receptor (TCR) signaling pathway) and the TCR come together after TCR triggering are not well understood. We investigate here the role of SNARE proteins, which are part of protein complexes involved in the docking, priming and fusion of vesicles with opposing membranes, in this process. Here we found, by silencing approaches and genetically modified mice, that the vesicular SNARE VAMP7 was required for the recruitment of Lat-containing vesicles to TCR-activation sites. Our results indicated that this did not involve fusion of Lat-containing vesicles with the plasma membrane. VAMP7, which localized together with Lat on the subsynaptic vesicles, controlled the phosphorylation of Lat, formation of the TCR-Lat-signaling complex and, ultimately, activation of T cells. Our findings suggest that the transport and docking of Lat-containing vesicles with target membranes containing TCRs regulates TCR-induced signaling.
- Published
- 2013
35. Human inflammatory dendritic cells induce Th17 cell differentiation
- Author
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Antonio Cappuccio, Gilles Chiocchia, Maxime Touzot, Sebastian Amigorena, Elodie Segura, Marc Dalod, Vassili Soumelis, Anne Hosmalin, and Armelle Bohineust
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Cellular differentiation ,Immunology ,Population ,Inflammation ,chemical and pharmacologic phenomena ,Biology ,Lymphocyte Activation ,Monocytes ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,medicine ,Immunology and Allergy ,Humans ,Secretion ,education ,Th1-Th2 Balance ,Cells, Cultured ,030304 developmental biology ,0303 health sciences ,education.field_of_study ,Macrophages ,Interleukin-17 ,hemic and immune systems ,Cell Differentiation ,Dendritic Cells ,3. Good health ,Infectious Diseases ,Organ Specificity ,CD4 Antigens ,Th17 Cells ,Interleukin 17 ,Signal transduction ,medicine.symptom ,Transcriptome ,Immunologic Memory ,030215 immunology ,Signal Transduction - Abstract
SummaryDendritic cells (DCs) are critical regulators of immune responses. Under noninflammatory conditions, several human DC subsets have been identified. Little is known, however, about the human DC compartment under inflammatory conditions. Here, we characterize a DC population found in human inflammatory fluids that displayed a phenotype distinct from macrophages from the same fluids and from steady-state lymphoid organ and blood DCs. Transcriptome analysis showed that they correspond to a distinct DC subset and share gene signatures with in vitro monocyte-derived DCs. Moreover, human inflammatory DCs, but not inflammatory macrophages, stimulated autologous memory CD4+ T cells to produce interleukin-17 and induce T helper 17 (Th17) cell differentiation from naive CD4+ T cells through the selective secretion of Th17 cell-polarizing cytokines. We conclude that inflammatory DCs represent a distinct human DC subset and propose that they are derived from monocytes and are involved in the induction and maintenance of Th17 cell responses.
- Published
- 2012
36. Human Primary Immune Cells Exhibit Distinct Mechanical Properties that Are Modified by Inflammation
- Author
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Bufi, Nathalie, primary, Saitakis, Michael, additional, Dogniaux, Stéphanie, additional, Buschinger, Oscar, additional, Bohineust, Armelle, additional, Richert, Alain, additional, Maurin, Mathieu, additional, Hivroz, Claire, additional, and Asnacios, Atef, additional
- Published
- 2015
- Full Text
- View/download PDF
37. Mechanical Characterization of Human Monocyte Derived Antigen Presenting Cells
- Author
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Bufi, Nathalie, primary, Bohineust, Armelle, additional, Dogniaux, Stéphanie, additional, Richert, Alain, additional, Saitakis, Michael, additional, Hivroz, Claire, additional, and Asnacios, Atef, additional
- Published
- 2014
- Full Text
- View/download PDF
38. Validation and Improvement of Body Panels FE Models from 3D-Shape and Vibration Measurements by Optical Methods
- Author
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Alexis Sol, Arnaud Lepage, Virginie Linet, and Xavier Bohineust
- Subjects
Vibration ,Materials science ,Electronic engineering ,Mechanical engineering ,Fe model - Published
- 2001
39. MAIT Cells Detect and Efficiently Lyse Bacterially-Infected Epithelial Cells
- Author
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Le Bourhis, Lionel, primary, Dusseaux, Mathilde, additional, Bohineust, Armelle, additional, Bessoles, Stéphanie, additional, Martin, Emmanuel, additional, Premel, Virginie, additional, Coré, Maxime, additional, Sleurs, David, additional, Serriari, Nacer-Eddine, additional, Treiner, Emmanuel, additional, Hivroz, Claire, additional, Sansonetti, Philippe, additional, Gougeon, Marie-Lise, additional, Soudais, Claire, additional, and Lantz, Olivier, additional
- Published
- 2013
- Full Text
- View/download PDF
40. MAIT Cells Detect and Efficiently Lyse Bacterially-Infected Epithelial Cells
- Author
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Mathilde Dusseaux, Philippe J. Sansonetti, Claire Hivroz, David Sleurs, Stéphanie Bessoles, Claire Soudais, Emmanuel Treiner, Marie-Lise Gougeon, Emmanuel Martin, Armelle Bohineust, Virginie Premel, Lionel Le Bourhis, Olivier Lantz, Nacer-Eddine Serriari, and Maxime Coré
- Subjects
Male ,Salmonella typhimurium ,Shigella dysenteriae ,QH301-705.5 ,T-Lymphocytes ,medicine.medical_treatment ,Immunology ,Mucosal associated invariant T cell ,Major histocompatibility complex ,medicine.disease_cause ,Microbiology ,Minor Histocompatibility Antigens ,Shigella flexneri ,Intestinal mucosa ,Immunity ,Virology ,Genetics ,medicine ,Humans ,Cytotoxic T cell ,Shigella ,Biology (General) ,Intestinal Mucosa ,Immunity, Mucosal ,Molecular Biology ,Dysentery, Bacillary ,biology ,Histocompatibility Antigens Class I ,Epithelial Cells ,RC581-607 ,biology.organism_classification ,Cytokine ,Salmonella Infections ,biology.protein ,Female ,Parasitology ,Immunologic diseases. Allergy ,Research Article ,NK Cell Lectin-Like Receptor Subfamily B - Abstract
Mucosal associated invariant T cells (MAIT) are innate T lymphocytes that detect a large variety of bacteria and yeasts. This recognition depends on the detection of microbial compounds presented by the evolutionarily conserved major-histocompatibility-complex (MHC) class I molecule, MR1. Here we show that MAIT cells display cytotoxic activity towards MR1 overexpressing non-hematopoietic cells cocultured with bacteria. The NK receptor, CD161, highly expressed by MAIT cells, modulated the cytokine but not the cytotoxic response triggered by bacteria infected cells. MAIT cells are also activated by and kill epithelial cells expressing endogenous levels of MRI after infection with the invasive bacteria Shigella flexneri. In contrast, MAIT cells were not activated by epithelial cells infected by Salmonella enterica Typhimurium. Finally, MAIT cells are activated in human volunteers receiving an attenuated strain of Shigella dysenteriae-1 tested as a potential vaccine. Thus, in humans, MAIT cells are the most abundant T cell subset able to detect and kill bacteria infected cells., Author Summary Human Mucosa-Associated Invariant T cells (MAIT) detect microbe-derived compounds presented by the MHC-like molecule, MR1. These foreign antigens are produced by a wide variety of microbes, including commensal and pathogenic bacteria or yeasts. MAIT cells expend shortly after birth and constitute the major antibacterial T cell subset described and, hence, could play important roles in infectious diseases. Here we show that MAIT cells recognize epithelial cells infected by the intestinal pathogen Shigella flexneri in a process requiring endogenous MR1, while the closely related bacterium Salmonella Tyhpimurium is not. Upon recognition, infected epithelial cells are efficiently lysed by MAIT cells. We also show that the triggering of CD161, a natural killer receptor highly expressed by MAIT cells, can modulate the cytokine but not the cytotoxic function of these cells. Finally, we provide evidence that MAIT cells are activated during the course of an experimental enteric infection in humans. Our study provides important insight on the antibacterial function of MAIT cells and their interaction with pathogenic bacterial species.
- Published
- 2013
41. Cytokine Secretion by CD4+ T Cells at the Immunological Synapse Requires Cdc42-Dependent Local Actin Remodeling but Not Microtubule Organizing Center Polarity
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Chemin, Karine, primary, Bohineust, Armelle, additional, Dogniaux, Stéphanie, additional, Tourret, Marie, additional, Guégan, Sarah, additional, Miro, Francesc, additional, and Hivroz, Claire, additional
- Published
- 2012
- Full Text
- View/download PDF
42. Crosstalk between T Lymphocytes and Dendritic Cells
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Hivroz, Claire, primary, Chemin, Karine, additional, Tourret, Marie, additional, and Bohineust, Armelle, additional
- Published
- 2012
- Full Text
- View/download PDF
43. Force Generation upon T Cell Receptor Engagement
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Husson, Julien, primary, Chemin, Karine, additional, Bohineust, Armelle, additional, Hivroz, Claire, additional, and Henry, Nelly, additional
- Published
- 2011
- Full Text
- View/download PDF
44. Development and application of holographic modal decomposition techniques to acoustic analysis of vehicle structures
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Virginie Linet, Xavier Bohineust, and Frederic Dupuy
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Engineering ,business.industry ,Optical engineering ,Holography ,Mechanical engineering ,Holographic interferometry ,Displacement (vector) ,law.invention ,Vibration ,Development (topology) ,Modal ,law ,Electronic engineering ,State (computer science) ,business - Abstract
The solution of structural-acoustic problems in vehicle development needs improvement of calculation and experimental dynamic analysis methods. In this aim, new analysis methods based on 1-D and 3-D displacement measurements recorded by pulsed holographic interferometry have been developed. These essentially allow to perform 3-D finite-element model updating, improvement of physical understanding for particular vibration phenomena and analysis of dynamic responses in running conditions. We discuss, in particular, two different original methods which make possible to determine the modal participations in a dynamic response measured in non-steady state conditions. Several research and industrial measurement applications are presented.© (1994) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
- Published
- 1994
45. T Cell Polarity at the Immunological Synapse Is Required for CD154-Dependent IL-12 Secretion by Dendritic Cells
- Author
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Tourret, Marie, primary, Guégan, Sarah, additional, Chemin, Karine, additional, Dogniaux, Stéphanie, additional, Miro, Francesc, additional, Bohineust, Armelle, additional, and Hivroz, Claire, additional
- Published
- 2010
- Full Text
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46. Force Generation upon T Cell Receptor Engagement
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Armelle Bohineust, Karine Chemin, Julien Husson, Claire Hivroz, Nelly Henry, Univers, Transport, Interfaces, Nanostructures, Atmosphère et environnement, Molécules (UMR 6213) (UTINAM), Université de Franche-Comté (UFC), and Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)
- Subjects
CD3 Complex ,Science ,T-Lymphocytes ,Immune Cells ,T cell ,Immunology ,Receptors, Antigen, T-Cell ,Biophysics ,Biology ,Major histocompatibility complex ,Antibodies ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Humans ,Biomechanics ,Biomacromolecule-Ligand Interactions ,Antigen-presenting cell ,Cytoskeleton ,Immune Response ,Actin ,030304 developmental biology ,0303 health sciences ,Multidisciplinary ,Physics ,Work (physics) ,T-cell receptor ,[SPI.MECA.BIOM]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Biomechanics [physics.med-ph] ,Biomechanical Phenomena ,Cell biology ,Kinetics ,medicine.anatomical_structure ,[SDV.IMM.IA]Life Sciences [q-bio]/Immunology/Adaptive immunology ,CD18 Antigens ,biology.protein ,Medicine ,030217 neurology & neurosurgery ,Intracellular ,Research Article - Abstract
International audience; T cells are major players of adaptive immune response in mammals. Recognition of an antigenic peptide in association with the major histocompatibility complex at the surface of an antigen presenting cell (APC) is a specific and sensitive process whose mechanism is not fully understood. The potential contribution of mechanical forces in the T cell activation process is increasingly debated, although these forces are scarcely defined and hold only limited experimental evidence. In this work, we have implemented a biomembrane force probe (BFP) setup and a model APC to explore the nature and the characteristics of the mechanical forces potentially generated upon engagement of the T cell receptor (TCR) and/or lymphocyte function-associated antigen-1 (LFA-1). We show that upon contact with a model APC coated with antibodies towards TCR-CD3, after a short latency, the T cell developed a timed sequence of pushing and pulling forces against its target. These processes were defined by their initial constant growth velocity and loading rate (force increase per unit of time). LFA-1 engagement together with TCR-CD3 reduced the growing speed during the pushing phase without triggering the same mechanical behavior when engaged alone. Intracellular Ca 2+ concentration ([Ca 2+ ] i) was monitored simultaneously to verify the cell commitment in the activation process. [Ca 2+ ] i increased a few tens of seconds after the beginning of the pushing phase although no strong correlation appeared between the two events. The pushing phase was driven by actin polymerization. Tuning the BFP mechanical properties, we could show that the loading rate during the pulling phase increased with the target stiffness. This indicated that a mechanosensing mechanism is implemented in the early steps of the activation process. We provide here the first quantified description of force generation sequence upon local bidimensional engagement of TCR-CD3 and discuss its potential role in a T cell mechanically-regulated activation process.
- Published
- 2011
47. New technique of three-dimensional dynamic measurements based on multi-pulsed holographic interferometry
- Author
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Xavier Bohineust and Virginie Linet
- Subjects
Physics ,Optics ,Normal mode ,Deflection (engineering) ,business.industry ,Electronic speckle pattern interferometry ,business ,Holographic interferometry ,Double pulse - Abstract
Double pulse holographic interferometry (DPHI) ideally completes standard accelerometrical techniques used for dynamic analysis but has some limitations. We present in this paper the development of a new original method based on DPHI allowing the measurement of the 3-D displacement vectors field with multi-pulse recordings. The method is based on three illuminating beams and one viewing direction and multi-pulse recording is achieved by fast deflection of reference beams. Accuracy is discussed and validated in experimental dynamic conditions through the analysis of vibration modes of a sinusoidally excited cylinder.
- Published
- 1993
48. Analysis of Medium-Frequency Vibrations in a Frequency Range
- Author
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Ladevèze, P., primary, Rouch, P., additional, Riou, H., additional, and Bohineust, X., additional
- Published
- 2003
- Full Text
- View/download PDF
49. Validation and Improvement of Body Panels FE Models from 3D-Shape and Vibration Measurements by Optical Methods
- Author
-
Linet, Virginie, primary, Lepage, Arnaud, additional, Sol, Alexis, additional, and Bohineust, Xavier, additional
- Published
- 2001
- Full Text
- View/download PDF
50. Development and application of holographic modal decomposition techniques to acoustic analysis of vehicle structures
- Author
-
Bohineust, Xavier, primary, Linet, Virginie, additional, and Dupuy, Frederic, additional
- Published
- 1994
- Full Text
- View/download PDF
Catalog
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