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Background. The cytoskeletal protein β-tubulin class III (Tubb3) is associated with tumor resistance to taxanes and vinca alkaloids, as well as with the metastatic potential of neoplasm, however, data from immunohistochemical analysis of Tubb3 expression in non-small cell lung cancer (NSCLC) tissue are few and contradictory. Purpose. Characterization of the level and intensity of Tubb3 expression in NSCLC tissue and analysis of the identified parameters correlation with clinically significant characteristics of the disease. Methods. Quantitative assessment of the level and intensity of Tubb3 expression in 120 surgical samples of NSCLC was carried out by immunofluorescence method associated with flow cytometry. Primary rabbit monoclonal antibodies specific to Tubb3 and secondary anti-rabbit antibodies conjugated with fluorescent dye DyLight650 (ab98510, UK) were used. The expression of the marker was assessed by two parameters: the level of expression measured as the percentage of the cells expressing Tubb3 and the intensity of expression in conventional units (CU) represented as the ratio of the geometric mean fluorescence intensity in the experimental and control samples (cells incubated with secondary antibodies only). Results. 1. Tubb3 expression was detected in all NSCLC samples studied. The median level and intensity of Tubb3 expression was 30.5% and 2.0 CU with significant differences (up to 10 times) in the quantitative values of both parameters in different patients. 2. The distribution of the studied tumors in terms of the level and intensity of Tubb3 expression differs from normal (P

Digital papillary adenocarcinoma (DPA ) is a rare malignant neoplasm of sweat glands, which was first described by Helwig in 1979 and then classified by Kao in 1987. This disease most often occurs in men aged 50–70 years and is characterized by a relatively favorable prognosis. In most cases, radical excision of the tumor leads to cure. However, 14–47 % of patients develop distant metastases with predominant lung involvement (70 %). The study of etiology and pathogenesis of this rare cancer and its molecular genetic profile seems to be interesting. Currently, there is no clear approach to the treatment of metastatic DPA , but sporadic cases of using chemotherapy have been reported. Aim of the study: to analyze current data on the pathogenesis of DPA , diagnostic features and treatment methods used, as well as to present the first clinical case of treatment of disseminated digital carcinoma with immune checkpoint inhibitors described in scientific literature. Material and Methods. A search of available literature published in Medline, Pubmed, etc. databases from 1984 to 2023 was performed, 21 sources were included in this review. Clinical Case Description. We present a rare case of metastatic digital papillary carcinoma in a 23-year-old male with disease manifestation at the age of 14 years. Lack of vigilance and awareness of oncologists and morphologists did not allow timely diagnosis of malignancy, even in case of 3 local recurrences of the disease. Only biopsy of a metastatic lung nodule with histological and IHC examination (of archival and new material) made it possible to make a correct diagnosis. This case revealed 2 potential targets that could be used for disease control: androgen receptor positive expression and PD -L1 expression with CPS =20. The use of immune response checkpoint inhibitors (ipilimumab + nivolumab) resulted in partial response followed by stable disease.

Abstract We extend the derivation of mass formulas for stationary axisymmetric asymptotically locally flat solutions with string singularities on the polar axis to general supergravity actions containing vector and scalar fields. It is based on the rod structure of the solutions in Weyl coordinates and is applicable to black holes with Dirac and Misner strings. The obtained formulas differ from the corresponding ones in Einstein–Maxwell theory only by summation over all independent electric charges.

Background. Progesterone receptors (PR) are regulators of cell proliferation and therefore can be considered as an aim for targeted medications in the treatment of oncological diseases. At the same time, a quantitative assessment of PR expression in the tissue of non-small cell lung cancer (NSCLC), which has not yet been carried out in other studies, will determine the possibility of using PR modulators for the treatment of this disease and identify the potential category of patients most susceptible to these drugs.Purpose. To characterize NSCLC by quantitative indicators of PR expression and to determine the correlation of clinically significant characteristics of patients and clinical and morphological parameters of a NSCLC tumor with the PR expression to assess the possibility of using PR modulators in the treatment of this disease.Methods. The PR expression in 130 surgical samples of NSCLC was quantified using an immunofluorescence method associated with flow cytometry. Primary antibodies to PR (NBP2-46388) and secondary antibodies conjugated with DyLight650 (ab98729) were used.Results. The expression of progesterone receptors was detected in all the studied tumors; an abnormal distribution of the marker expression level was noted (P=0.01). The mean expression level was 55.3±16.2%, and the median was 57% with a range of 70%, which indicates heterogeneity of PR expression in tumors of different patients. There were no statistically significant differences in the level of PR expression depending on the histotype and stage of NSCLC, as well as on the sex of patients. At the same time, the level of expression and the frequency of overexpression of PR (>67%) in tumors in non-smoking patients are higher than in smokers (P⩽0.02).Conclusion. The high frequency of occurrence and level of PR expression in NSCLC indicate the possible effectiveness of the use of their modulators in the treatment of this disease, especially in non-smoking patients.

Technologies for the manufacture of microwave electromagnetic shields based on powdered carbon, foil and composite multilayer materials are presented. Technological methods for their manufacture and shielding properties are described, and the main mechanisms leading to increased shielding properties are considered depending on the manufacturing technology, composition and alternation of composite layers. It is shown that the values of the reflection coefficient of electromagnetic radiation in the frequency range 2–17 GHz for the shields considered reach a value of (–20) dB. Microwave absorbers with a reflection characteristic lower than (–10.0) dB (down to (–52.0) dB) in the frequency band 8.0–12.0 GHz are developed, and the electrophysical parameters, composition and concentration of components of the composite materials for such microwave absorbers are determined. The systems for active protection of speech information that use combined masking signals consisting of “white” noise and speech-like signals as masking signals, along with “white” noise are considered. Criteria have been developed for the approach to selecting speakers and audiences when assessing speech intelligibility to solve problems of protecting speech information and methods for experimental studies of speech intelligibility against a background of masking acoustic noise. Technologies for the formation of nanocomposites based on carbon nanotubes and nanoparticles of ferromagnetic materials are presented to increase the degree of coherence of the spin texture at macroscopic distances, increase the degree of security of electronic products to strong magnetic fields and noise immunity. It has been shown that at certain concentrations of ferromagnetic nanoparticles, micromagnetic parameters increase, ensuring high performance of active elements in magnetic fields. The role of carbon in this case turns out to be decisive. The presented results seem promising for use in the creation of anechoic chambers, partitions for separating indoor zones intended for the location of electronic devices, products for protecting humans from the effects of electromagnetic radiation in the microwave range, and the formation of combined masking signals in the form of “white” noise for devices protection of speech information, composite coatings to level out the effects of a constant magnetic field on electronic products.

Abstract Massive particle surface is a generalization of the photon surface, both of which translate the dynamical properties of geodesics orbiting the gravitating object onto the geometric description. The procedure for constructing the massive particle surfaces in static space-times is described in detail and the equivalence of the main results with the results of the geodesic approach is demonstrated for the following examples: Schwarzschild, Reissner–Nordström, dilatonic black holes, conformal gravity, and Culetu model.

The paper deals with technology of manufacturing of microwave absorbers with ordered and structured surface layer. This layer contains Möbius strip resonators, located on the fixed distance from each other. The technology was developed by the authors on the base of the following data: 1) results of theoretical study of microwaves interaction with Möbius strip resonator; 2) results of experimental study of electromagnetic radiation reflection, transmission and absorption coefficients in the microwave frequency range of the experimental samples of microwave absorbers based on Möbius strip resonators, which differ by the distance between these resonators. It was established on the base to the listed results that the microwave absorbers manufactured in accordance to the technology proposed by the authors are wideband ones. Electromagnetic radiation absorption coefficient values of these absorbers changes from 0.5 to 0.97 in frequency range 2.0–17.0 GHz if the distance between the Möbius strip resonators containing in their surface layer, is 15.0 mm. If the distance between the Möbius strip resonators containing in the surface layer of the considered absorbers is less than 15.0 mm their electromagnetic radiation absorption coefficient in the indicated frequency bands is lower than presented values. The considered absorbers are promising for use in shielded rooms and anechoic chambers.

This study introduces a novel S-nitrosothiol based method for the rapid and highly selective detection of nitrite in complex water matrices. Sodium 3-mercapto-1-propanesulfonate forms a distinctive pink S-nitrosothiol compound upon interaction with nitrite in acidic media, allowing both visual and quantitative detection. Various factors affecting the absorbance of the final product were investigated, including pH, reaction time, acid type, and sodium 3-mercapto-1-propanesulfonate concentration. UV–Vis spectrophotometric analysis demonstrated an excellent linear correlation (R2 = 0.99) across a broad detection range (0.05 to 80 mmol l-1), while showing no interference from common ions such as nitrate or dissolved organic matter, a limitation frequently observed in conventional UV-based nitrite detection methods. The assay was further adapted into a pellet form to simplify field use, operating effectively at room temperature with a low detection limit (1.4 ppm). The S-nitrosothiol based method represents a safer and more environmentally friendly option for nitrite detection and shows a promising potential as a valuable addition to both field and laboratory water testing kits for nitrite analysis.

The aim of the study is to analyse the educational means facilitating the development of the linguocultural competence within the academic environment in Ukraine under conditions of the coronavirus pandemic. About 60 senior students and 15 university instructors giving classes in English and Chinese participated in the research. The research methodology is based on analytical and practical analyses of the normative documents facilitating the correlation of foreign language education standards in Ukraine, Europe and China; interviewing; observation (within the experimental study/training). The results show that all students and instructors admitted the importance of culture-related issues in the process of teaching and learning languages; 99% of the teaching staff and 75% of the students chose a more detailed content of culture-related issues to be taught/learnt; 80% of instructors and 78% of the students highlighted the importance of organising culture-related events. The research results show that it is crucial to accomplish a balanced integration of language and culture in the teaching process. Teachers should clearly understand what cultural information is significant and how to make efficient use of it by applying digital resources. The key issues teachers should pay attention to are the evaluation of cultural information, its accessibility and the most efficient ways to present it to students.

The melanoma origin of cell lines obtained from the axillary lymph node (mel Kas, mel Pet, and mel Lap from patients with a verified diagnosis) was confirmed by the detection of the Melan A melanocyte marker expression. A hyperdiploid (2n+) for the mel Kas line; near-diploid (2n), and in some cells near-tertaploid (4n), and even hypo-octaploid (8n) set (172–179 chromosomes) in the mel Pet cell line; and a hypotetraploid (4n−) for the mel Lap line were detected by karyotypic analysis. All three cell lines are tumorigenic; however, mel Pet demonstrates tumor growth in Balb/c nude mice only in the presence of matrigel. All three lines showed a high expression of TUBB3 and PD-L1 markers, while ERa was low (minimum for mel Pet). Significant differences in the expression level were shown for the Cyt molecular marker. In the transplantation of cells to Balb/c nude mice, a stable expression level is observed only for TUBB3. For the rest of the markers, a decrease in their expression level of varying degrees was noted when the cells were growing in solid tumors in vivo. Mutations were detected in oncogenes (BRAF, EZH2, KIT, KRAS, NRAS, ROS1) and tumor suppressor genes (CDKN2A, FAT4, KMT2C, LRP1B, PTEN, PTPRB, TP53). The detailed characterization of the cell lines makes them valuable for various scientific and regulatory experiments, particularly those involving preclinical data on antiproliferative drugs for malignant melanoma or investigations into melanoma cell properties and progression.

The article reveals the essence of the phenomenon "professional culture of future educators of preschool educational institutions" viewed as a complex integral formation within a holistic structure of a personality. It is characterized by a positive attitude to the subjects and objects of pedagogical activity (axiological component), deep and systematized professional knowledge, skills and competencies (cognitive component), as well as personal and professional qualities that reflect the level of mastery of a special area of knowledge (personal component) and find their manifestation in pedagogical activity (reflexive component). Criteria (specified by indicators) that characterize levels of professional culture development of future preschool educators have been determined, including: informational, motivational, behavioral and appraisal-creative. Pedagogical conditions for the formation of professional culture of future preschool educators in the process of their professional training have been substantiated. The above-mentioned pedagogical conditions presuppose: activation of educational activities of future preschool educators in the cultural and educational environment of a higher pedagogical institution; provision of interactive technological support for the educational process of future preschool educators; integration of the content of normative professional disciplines. The technique of forming the professional culture of future educators of preschool educational institutions is presented.

Background. Estrogen receptors beta (ERβ) are an important biological regulator and target of antiestrogens, however, unlike estrogen receptors alpha (ERɑ), their significance in the prognosis and treatment of breast cancer remains unclear. Purpose. Evaluation of the ERβ prognostic value in the comparative assessment of frequency and level of the marker expression in groups with good and poor prognosis by Ki-67 proliferative index score in breast cancer. Methods. ERβ expression level (% of cells expressing the marker) in 68 breast tissue samples was quantified by immunofluorescence and flow cytometry. Primary antibodies to ERβ (clone 14C8, ab288) and secondary antibodies conjugated with DyLight650 (ab98729) were used. In the same samples, the Ki-67 expression level was assessed by the immunohistochemical method. Results. The ERβ and Ki-67 were detected in 100% breast tissue samples with high heterogeneity of the markers’ expression in different patients. Statistical analysis of good and poor prognosis in accordance with the Ki-67 proliferative index score (Ki-67≤20% and Ki-67>20%) showed the prognostic value of the ERβ expression level of 50%. There was no association between the Ki-67 and ERβ expression levels in the same tumor sample (Spearman's rank correlation coefficient R=–0,16; P>0,05). At the same time, high expression of ERβ≥50% was 2,3 times more frequently detected in the good vs poor prognostic group by Ki-67 — 41% vs 18%, P=0,02. Conclusion. The ERβ expression level ≥50% in the tumor can be considered as a factor of good prognosis of breast cancer.

The purpose of the article is to identify the scale of undeclared labour in Ukraine and to study the problems, principles and prospects of its prevention in unstable socio-economic conditions. Methodology. In line with the EU and ILO methodology, the State Labour Service of Ukraine interprets undeclared labour as legal paid activity that is not fully or partially registered in accordance with the law. Results. Undeclared work plays an important role in the practice of business management and competitiveness stimulation in countries with industrial, neo-industrial and post-industrial economies, which has been developed almost since the middle of the 20th century. The paper proves that undeclared work is a broader concept that includes legalised (both official and manipulative) as well as shadowy mechanisms of its occurrence and functioning. Given the problematic performance by the State Labour Service of Ukraine of its inspection and sanctioning powers, as well as the widespread use of non-standard labour relations (with mediation in the performance of employer functions, outsourcing of auxiliary, service and some specialised personnel to companies), tariff-free remuneration systems encourage numerous manipulations in the area of labour declaration and decent wages. The source of problems with the spread of remuneration systems based on an assessment of the complexity of the work performed and the qualifications of employees, as well as with ensuring decent working conditions for agency (loan, temporary) workers, is the unclear legislative regulation of these systems. Practical implications. The priorities for preventing undeclared labour in Ukraine are: a consistent increase in the share of wages in the structure of production costs; strengthening cross-control over the fulfilment of obligations to pay the minimum wage under the tariff-free and hourly wage systems; and the introduction of a sound methodology for harmonising the minimum wage, the subsistence minimum and other basic social standards linked to them. Value/Оriginality. The expediency of identifying institutionalised and manipulative mechanisms of undeclared labour, their formal and illegal bases is confirmed by the classification of the main forms of undeclared labour used by the EU, ILO and the State Labour Service of Ukraine; this approach will increase the complexity and effectiveness of systemic measures to regulate it (control, mitigation, prevention).

The interaction of radio pulse signals in an anisotropic medium over hydrocarbon deposits has been studied. During the experiments, computer simulation of the characteristics of anisotropic media was performed. The dependences of the real, imaginary, and phase components of the characteristics of the surface impedance of an anisotropic medium over hydrocarbons on its dielectric constant, the frequency of radio pulse signals interacting with it, and the number of spectrum harmonics of the latter are obtained. Based on the research results, recommendations are given to improve the accuracy of determining the boundaries of hydrocarbon deposits using electrical exploration methods based on the use of radio pulse signals. The research results can be applied in prospecting geophysics for the discovery of oil and gas deposits.

Introduction. Melanoma belongs to the group of the most malignant tumors characterized by aggressive growth and active metastasis. At the same time, the effectiveness of therapy, primarily targeted therapy, is largely limited by the rapid development of drug resistance.Aim. To study the effect of chronic ultraviolet (UV) irradiation on the formation of a population of radiation-resistant melanoma cells; to study the features of cell signaling and the sensitivity of UV-resistant melanoma cells to the antitumor drugs.Materials and methods. The experiments were carried out on in vitro cultured A375 melanoma cells. Cells were cultured in a standard DMEM + 10 % FBS medium; cell growth rate was analyzed using the MTT assay; cell survival after irradiation was analyzed using a colony-forming test. Determination of the transcriptional activity of the estrogen receptor (ER) was performed by reporter analysis upon transfection into cells of a plasmid containing the luciferase reporter gene controlled by estrogen responsive element. The immunoblotting method was used to analyze the expression of cellular proteins; comparative analysis of ERα and ERβ expression was performed by immunofluorescent method.Results. Long-term UV irradiation leads to the formation of a UV-resistant subpopulation of A375 melanoma cells, which is characterized by decreased sensitivity to targeted (vemurafenib) and hormonal (tamoxifen) drugs, increased expression of Snail, an activator of the epithelial-mesenchymal transition, and in the absence of noticeable changes in the expression of PI3K / mTOR signaling. Metformin reduces Snail expression in both parental and UV-resistant A375 cells and enhances the cytostatic effect in combination with vemurafenib or tamoxifen.Conclusion. The data obtained demonstrate a decrease in the sensitivity of melanoma cells to targeted drugs under the long-term exposure to UV. The ability of metformin to potentiate the action of targeted drugs and inhibit Snail allows us to consider metformin not only as an antitumor agent, but also as a potential inhibitor of the epithelial-mesenchymal transition.

Introduction. Despite advances in the treatment of melanoma, the results of therapy cannot be considered satisfactory, and the search for new drugs and effective combinations of medicine continues. The drugs are being developed aimed at reducing the metastatic tumor potential – migrastatics. The targets of the drugs can be cytoskeletal proteins of tumor cells – cytokeratin (CK) intermediate filaments and microtubule protein beta-III tubulin (TUBB3).Aim. To estimate of the CK and TUBB3 expression in melanoma cell lines to form an informative in vitro cell model for screening and studying migrastatics.Materials and methods. The molecular phenotype of 21 human melanoma cell lines from the collection of N. N. Blokhin National Medical Research Center of Oncology, and 18 of which were isolated from tumor metastases in the lymph nodes, soft tissues or subcutaneously. The level of TUBB3 expression and de novo expression of CKs in vimentin-expressing cells (CK + Vim) were assessed by an immunofluorescent method and flow cytometry.Results. Beta-III tubulin expression was detected in all cultures studied, de novo expression of CKs was found in 20 / 21 lines. The exception was primary uveal melanoma 92-1, that did not express CK + Vim. Both parameters significantly differed between the cells of the studied panel: CK + Vim co-expression – from 0 to 91 %, TUBB3 – from 18 to 86 %. No correlation was found between the expression level of TUBB3 and CK + Vim (Pearson’s correlation coefficient r = 0.11; p = 0.65). Three groups of the cell lines with different ratio of TUBB3 expression and CK + Vim co-expression were identified: 1) similar level of expression of both markers; 2) the level of co-expression of CK + Vim more or less high than the index for TUBB3; 3) the level of TUBB3 expression more or less high than the index for CK + Vim co-expression.Conclusion. A panel of 21 human melanoma cell lines was formed with quantitatively estimated expression of cytoske-letal proteins responsible for the migration activity of tumor cells – CKs and TUBB3. Groups of the lines with different expression ratio of the markers can be used for screening and preclinical evaluation potential migrastatics that reduce the metastatic potential of melanoma and may reduce resistance to taxanes.

The article discusses the problem of implementing an integrated approach in the system of preschool education at the present stage both in preschool education institutions and in the system of higher school. Why did it become necessary to reconsider this problem, the relevance of which was discussed at length in the early twenty-first century? The appeal to the organization of the educational process in the preschool education system of Ukraine is connected with the adoption of the Concept "New Ukrainian School", which states that the leading principle of the content of education in primary school is the integration of the content of related new modules from other subjects (NUS, 2016: 25). The implementation of the principle of continuity in the organization of the educational process in preschool education has again raised the issue of intensifying the integrated approach in the system of preschool education, the core of which is the principle of integration. The article presents the results of the analysis of the scientific and terminological field of the concept of "integration" in terms of the variety of its interpretations and terminology in the definition of methodological approaches (integration, integrative, integral, integrated); specifies the application of the integrated approach in the modern educational field of both preschool education and higher school. Purpose of the article: to characterize the current state of implementation of the integrated approach in the modern practice of preschool education. The objectives of the study are to analyze the views of scientists on their understanding of the content position of scientific concepts that are components of the integrated approach. To focus the current practical application of the integrated approach in the system of preschool education.