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Background:Cuproptosis is a novel form of cell death, which is triggered by targeted mitochondria that bind the acylated lipid components of the tricarboxylic acid (TCA) cycle. Recent studies have suggested that targeting cuproptosis may be a new treatment strategy for cancer, but its association with cervcal cancer (CC) has not been elucidated. Methods: Data were downloaded from the TCGA-CESC. The cuproptosis-related lncRNAs were identified and prognostic risk models were constructed. Kaplan-Meier survival analysis, ROC curve, PCA and stratified analysis were used to validate the predictive ability of the model. A nomogram for predicting CC prognosis was established. The biological mechanisms associated with cuproptosis were investigated by GO and KEGG enrichment analysis. The CIBERSORT algorithm, immunoassay and TIDE score were used to measure different immune responses. Finally, we screened for sensitive drugs based on cuproptosis correlation. Results: We constructed a cuproptosis-related lncRNAs signature as a risk prognostic prediction model. The risk score was proved and the risk model had higher diagnostic efficiency than other features. The Kaplan-Meier curve indicated that lower risk score was significantly associated with better OS rate in training (PConclusions: Our study reveals that assessing cuproptosis patterns in individual tumors will help improve our understanding of the immune mechanisms and prognosis of cervical cancer and thus guide immunotherapy more effectively. Establishing an independent prognostic model based on cuproptosis-associated lncRNAs could serve as a predictor of overall survival and also as a predictor of immunotherapy. The model may help provide personalized medical therapy and new insights into cancer treatment.

This study describes a novel series of UDP-N-acetylglucosamine acyltransferase (LpxA) inhibitors that was identified through affinity-mediated selection from a DNA-encoded compound library. The original hit was a selective inhibitor of Pseudomonas aeruginosa LpxA with no activity against Escherichia coli LpxA. The biochemical potency of the series was optimized through an X-ray crystallography-supported medicinal chemistry program, resulting in compounds with nanomolar activity against P. aeruginosa LpxA (best half-maximal inhibitory concentration (IC50) 20 μM and MIC > 128 μg/mL). The mode of action of analogues was confirmed through genetic analyses. As expected, compounds were active against multidrug-resistant isolates. Further optimization of pharmacokinetics is needed before efficacy studies in mouse infection models can be attempted. To our knowledge, this is the first reported LpxA inhibitor series with selective activity against P. aeruginosa.

This paper designs a virtual SDN network management model constrained by fair and equal network management information access mechanisms by analyzing the problems existing in the universality of existing SDN network management models. Starting with the three-tier structure of the SDN network management system, the main parameters involved in the network management service function, information processing and transmission channel construction in the system were strictly and normatively defined. The design of virtual nodes is regarded as the core element of the network management system, and the information transmission inside it adopts logical operation; The network management service function and the channel for realizing the network management service function are isolated, and the iterative search, analysis and update mechanism is enabled in the network management information transmission channel. By constructing the experimental verification platform and setting the evaluation parameters of the system performance objectives, the scalability and timeliness of the model were evaluated from two aspects: the deployment of network virtual nodes and the dynamic control of network management information channels. The collected experimental core evaluation parameters, the realization time of the network management service function, can show that the dynamic distribution mechanism of network management information can be cross-applied to each virtual node, and the channel update mechanism of network management information can adjust the information processing queue in real-time. The network management system model that has been built realizes the separation of management and control of the network management system and has the characteristics of independent operation, autonomous function, self-matching, rapid deployment and dynamic expansion.

This study describes a novel series of UDP

Additional file 1: Supplemental Table 1. Pregnancy outcomes among different infertility etiologies for IVF compared to spontaneous pregnancies in twins. Supplemental Table 2. Neonatal outcomes among different infertility etiologies for IVF compared to spontaneous pregnancies in Twins. Supplemental Table 3. cORs, aORs and 95% CIs of pregnancy outcomes among different infertility etiologies for IVF compared to spontaneous pregnancies in Twins. Supplemental Table 4. cORs, aORs and 95% CIs of neonatal outcomes among different infertility etiologies for IVF compared to spontaneous pregnancies in Twins

Background To examine differences in the maternal characteristics and pregnancy outcomes of Chinese women with various causes of infertility who underwent in vitro fertilization (IVF) with embryonic cryopreservation treatment. Methods Cases were pregnancies after IVF-ET with embryonic cryopreservation; controls were spontaneously conceived pregnancies. Subgroup analysis was carried out according to etiology of infertility. The IVF treatment group was divided into 5 subgroups according to infertility etiology as follows: ovulation disorder, tubal disease, male infertility, endometriosis, and mixed infertility. Data on demographic characteristics, medical history, laboratory tests, and delivery were reviewed. Logistic regression analysis was performed for pregnancy and perinatal complications and neonatal outcomes. The multivariable model was adjusted for potential confounders. Results Among singleton pregnancies, compared with spontaneous pregnancies, IVF pregnancies were associated with significant increases in the rates of the following: gestational diabetes mellitus (GDM) (aOR 1.76[95% CI 1.33–2.33]), preeclampsia (2.60[1.61–4.20]), preterm preeclampsia (4.52[2.03–10.06]), postpartum hemorrhage (1.57[1.04–2.36]), intrahepatic cholestasis of pregnancy (3.84[1.06–13.94]), preterm premature rupture of membranes (2.11[1.17–3.81]), preterm birth (1.95[CI 1.26–3.01]), low birthweight (1.90[1.13–3.20]), macrosomia (1.53[1.03–2.27]), and neonatal intensive care unit (NICU) admission (1.69[1.22–2.34]) in the ovulation disorder group; GDM (1.50[1.21–1.86]), placenta previa (2.70[1.59–4.59]), placenta accreta (1.78[1.10–2.89]), postpartum hemorrhage (1.61[1.19–2.18]), macrosomia (1.60[1.21–2.13]) and 5-min Apgar score ≤ 7 (4.09[1.04–16.08]) in the tubal disease group; placenta previa (9.33[4.22–20.62]), small for gestational age (2.29[1.04–5.08]), macrosomia (2.00[1.02–3.95]) and NICU admission (2.35[1.35–4.09]) in the endometriosis group; placenta previa (4.14[2.23–7.68]) and placenta accreta (2.05[1.08–3.87]) in the male infertility group; and GDM (1.85[1.15–2.98]), placenta previa (4.73[1.83–12.21]), placental abruption (3.39[1.20–9.56]), chorioamnionitis (2.93[1.04–8.26]), preterm birth (2.69[1.41–5.15]), and 1-min Apgar score ≤ 7 (4.68[1.62–13.51]) in the mixed infertility group. Among multiple pregnancies, most of the differences that were significant in singleton pregnancies were less extensive or had disappeared. Conclusions Infertility etiology within the IVF population was found to affect maternal and neonatal outcomes among all births. During the perinatal period, infertility etiology appears to be an additional risk factor for abnormal pregnancy outcomes besides the use of IVF techniques compared with spontaneous pregnancies. Higher risk was found for ovulation disorders, and lower risk was found for male infertility.

Background: To examine differences in the maternal characteristics and pregnancy outcomes of Chinese women with various causes of infertility who underwent in vitro fertilization(IVF) with embryonic cryopreservation treatment.Methods: This retrospective cohort study included women with various causes of infertility who used IVF. In order to reduce the research error, we deliberately removed the fresh ET cycles and ICSI cycles at the beginning, so in our data the IVF-ET method was frozen-embryo transfer.[MOU1] [MOU2] Data on demographic characteristics, medical history, laboratory tests, and delivery were reviewed. Logistic regression analysis was performed to calculate odds ratios and 95% confidence intervals for pregnancy and perinatal complications and neonatal outcomes. The multivariable model was adjusted for age, gravidity, parity, pre-pregnancy obesity, birth plurality, and history of previous caesarean section.[MOU3] Results: The IVF treatment group was divided into 5 subgroups according to infertility cause as follows: ovulation disorder, tubal disease, male infertility, endometriosis, and mixed infertility. Among singleton pregnancies, we verified that IVF with embryonic cryopreservation pregnancies are related to increased risks of adverse perinatal outcomes. Besides，compared with spontaneous pregnancies, IVF pregnancies were associated with significant increases in the rates of the following: gestational diabetes mellitus (GDM), preeclampsia, preterm preeclampsia, postpartum haemorrhage, intrahepatic cholestasis of pregnancy, preterm premature rupture of membranes, preterm birth, low birthweight, macrosomia, and neonatal intensive care unit (NICU) admission in the ovulation disorder group; GDM, placenta previa, placenta accreta, postpartum haemorrhage, macrosomia and 5-minute Apgar score ≤7 in the tubal disease group; placenta previa, small for gestational age, macrosomia and NICU admission in the endometriosis group; placenta previa and placenta accreta in the male infertility group; and GDM, placenta previa, placental abruption, chorioamnionitis, preterm birth, and 1-minute Apgar score≤7 in the mixed infertility group. Among multiple pregnancies, most of the differences that were significant in singleton pregnancies were less extensive or had disappeared.[MOU4] [MOU5] Conclusions: During the perinatal period, maternal characteristics, in particular type of infertility, appears an additional risk factor for abnormal pregnancy outcomes besides use of IVF techniques. Lower risk is found in male infertility and higher risk for ovulation disorders.[MOU6]

Background : To examine differences in the maternal characteristics and pregnancy outcomes of Chinese women with various causes of infertility who underwent assisted reproductive technology (ART) treatment. Methods: This retrospective cohort study included women with various causes of infertility who used ART. Data on demographic characteristics, medical history, laboratory tests, and delivery were reviewed. Logistic regression analysis was performed to calculate odds ratios and 95% confidence intervals for pregnancy and perinatal complications and neonatal outcomes. The multivariable model was adjusted for age, gravidity, parity, pre-pregnancy obesity, birth plurality , and history of previous caesarean section. Results: The ART treatment group was divided into 5 subgroups according to infertility cause as follows: ovulation disorder, tubal disease, male infertility , endometriosis, and mixed infertility . Among singleton pregnancies, compared with spontaneous pregnancies , ART pregnancies were associated with significant increases in the rates of the following: gestational diabetes mellitus (GDM), preeclampsia, preterm preeclampsia, postpartum haemorrhage , intrahepatic cholestasis of pregnancy , preterm premature rupture of membranes, preterm birth, low birthweight, macrosomia, and neonatal intensive care unit (NICU) admission in the ovulation disorder group; GDM, placenta previa, placenta accreta , postpartum haemorrhage, macrosomia and 5 -minute Apgar score ≤7 in the tubal disease group; placenta previa, a bnormal placental cord insertion , small for gestational age, macrosomia and NICU admission in the endometriosis group; placenta previa and placenta accreta i n the male infertility group; and GDM, placenta previa, placental abruption, chorioamnionitis, preterm birth , and 1-minute Apgar score≤7 i n the mixed infertility group. Among multiple pregnancies, most of the differences that were significant in singleton pregnancies were less extensive or had disappeared. Conclusions: During the perinatal period, maternal characteristics, in particular type of infertility, appears an additional risk factor for abnormal pregnancy outcomes besides use of IVF techniques. Lower risk is found in male infertility and higher risk for ovulation disorders.

Background: To examine differences in maternal characteristics and pregnancy outcomes among Chinese women with infertility due to various reasons who underwent assisted reproductive technology(ART). Methods: This retrospective cohort study included women who used ART for various reasons. Demographics, medical history, laboratory tests, and delivery data were reviewed. Results: The ART treatment group was divided into 5 subgroups according to reason for infertility: ovulation disorder, tubal disease, male factor, endometriosis, multiple infertility-related diagnoses. Compared with spontaneous pregnancies, significant increases in the following were observed with ART: rates of gestational diabetes mellitus(GDM), preeclampsia, preterm premature rupture of membranes, postpartum hemorrhage, and chorioamnionitis in ovulation disorder; GDM, placenta previa and postpartum hemorrhage in tubal disease; placenta previa in endometriosis and male factor; and GDM, placenta previa and chorioamnionitis in multiple infertility-related diagnoses. Preterm birth, low birthweight, and small for gestational age were increased in all ART groups. Macrosomia and neonatal intensive care unit admission were increased in maternal factors with ART. Conclusions: During the perinatal period, women with different reasons for undergoing ART have different maternal characteristics and infant outcomes when compared with women not using ART, perinatal morbidities are higher in women undergoing ART for most reasons. The worst and best prognoses for infertility were found for that caused by ovulation disorder and male factor, respectively.

Through the analysis of the composition of the hub general plan of train disintegration and assemblage system, and the operation flow of the three sub-systems such as arrive, disintegration and assemblage and departure, established an evaluation system covering the scale of capability, production efficiency, feasibility and sustainability. This paper gives the definitions and calculation methods of the 15 indicators of the evaluation system, and hope to provide reference for improving the planning and evaluation of the disintegration and assemblage system.

Organizing the direct train flow of the loading area can reduce the load of the technical station along the way, accelerate the turnover of wagons and the delivery of goods, and facilitate the better integration of cargo flow and train flow. By analyzing three typical car organization forms in loading area and the hourly consumption index of the train flow in the loading area, a 0-1 nonlinear optimization model for direct routes distribution of trains in railway loading areas is constructed, with the goal of minimizing the total vehicle hour consumption of the train flow, and is bound by the uniqueness of the car flow organization and the number of trains compiled by each loading area and technical station. According to the complex characteristics of the model and the characteristics of the constraints, an improved genetic algorithm that limit spatial search strategy can be designed. This algorithm ensures the spatial feasibility of the solution during the execution through the adaptive adjustment of the crossover operator and the mutation operator. Finally, the effectiveness of the algorithm and model is verified by the example study and the case study of Huangdao loading area.

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