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Background: Using anaesthetics is an important application in aquaculture especially where the fish transportation, vaccination, grading, sorting activities and many other handling operations have been conducted during the different stages of production periods in the farms or hatcheries., Objectives: This study aimed to evaluate the efficacy of oregano essential oil (OO) as an anaesthetic for Nile tilapia and to determine the optimal concentration and post-application stress effects compared to clove oil (CO)., Methods: Nile tilapia juveniles were exposed to different concentrations of OO (20-40-60-80-100 mg L -1 ) and CO (50 mg L -1 ) for different time periods to determine the optimal concentration and exposure time. After the effective concentration of OO was determined, in the second experiment, stress parameters (glucose, plasma cortisol) were analysed after 0, 2, 6, 12 and 24 h of application. The results compared to the control group and CO results., Results: The study found that OO which has carvacrol compound higher than 78% was an efficient anaesthetic for Nile tilapia, and the effective concentration was found to be 60 mg L -1 . No significant differences were found between opercular beats in any concentration of OO and CO. Basal glucose level in blood without anaesthesia application and recorded at 39.33 mg dL -1 and significantly lower than OO and CO at first two sampling points, 0 and 2 h (p < 0.05). According to plasma cortisol level results, although CO experimental group showed secondary stress response at 12 h (17.91 ± 4.21 ng mL -1 ), OO and CO group cortisol levels decreased at 24 h after anaesthesia application 7.13 ± 0.14and 7.01 ± 0.54 ng mL -1 , respectively, below the control group cortisol concentration (12.28 ± 1.81 ng mL -1 )., Conclusions: These findings have important implications for the aquaculture industry as the use of OO as an anaesthetic could reduce the stress and mortality associated with traditional anaesthetics. Further research is needed to evaluate the efficacy of OO as an anaesthetic agent for other fish species and to determine the optimal concentration and exposure time for different species., (© 2024 The Author(s). Veterinary Medicine and Science published by John Wiley & Sons Ltd.)

Due to the lack of environmental stimuli found in their natural habitat, not all species of tiny fish can reproduce naturally in the conditions of captivity. Silver rasbora (Rasbora argyrotaenia) was used as the model organism in this study, which is the first to successfully use the hormonal technique of immersion to mass induce spawning and spermiation in small fish species. In this study, in order to find out the effect of immersion hormone treatment, two separate experiments in which commercial hormone Ovaprim™ was used for spawning induction and Ovagold™ for spermiation induction were conducted. Four doses of hormones (0.0 mL/L, 0.7 mL/L, 1.4 mL/L, and 2.1 mL/L) were evaluated on 24 females and 40 males in total. According to the experimental findings of female breeders, 1.4 mL/L dose of Ovaprim™ showed best results on latency period (8.67 h) with a total number of eggs (6994 egg) and fertilization rate (99.22 %). In addition, hatching and larval survival rate have also increased. Milt volume and sperm concentration have also seen an increase to 4.25 μL/g bw and 42.71 10 6 cell/μL respectively at 2.1 mL/L hormone concentration. The best results or responses were obtained by immersion treatments using Ovaprim™ doses of 1.4 mL/L on female and Ovagold™ concentrations of 2.1 mL/L on male. This new technique is a beneficial for breeding small fish species for either commercial or conservational culture condition., Competing Interests: Declaration of Competing Interest On behalf of my co-authors, I am submitting the enclosed manuscript entitled "A novel technique for mass induction of spawning in small fish species: hormone immersion" for possible publication Animal Reproduction Sciences journal. All persons listed as authors have read, contributed to preparing the manuscript and attest to the validity and legitimacy of the data and its interpretation, and agree to its submission to Animal Reproduction Sciences. No person(s) more than the authors listed have contributed significantly to its preparation. The manuscript has not been submitted for publication nor has been published in whole or in part elsewhere. There is no competing interest to declare by the authors. We agree that the publisher and editors will not be responsible for errors have been made by us or any consequences arising from the use of information contained in this manuscript or our article will be probably published in your journal. On behalf of my co-authors and by sending this attachment, I agree all the terms mentioned above., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Objective: To compare the effect of microfluiding sperm sorting chip and density gradient methods on ongoing pregnancy rates (PRs) of patients undergoing IUI., Design: Retrospective cohort study., Setting: Hospital IVF unit., Patient(s): Couples with infertility undergoing IUI cycles between 2017 and 2018., Intervention(s): Not applicable., Main Outcome Measure(s): Ongoing PRs., Result(s): A total of 265 patients were included in the study. Microfluid sperm sorting and density gradient were used to prepare sperm in 133 and 132 patients, respectively. Baseline spermiogram parameters, including volume, concentration, motility, and morphology, were similar between the two groups. Total motile sperm count was lower in the microfluiding sperm sorting group at baseline (35.96 ± 37.69 vs. 70.66 ± 61.65). After sperm preparation sperm motility was higher in the microfluid group (96.34 ± 7.29 vs. 84.42 ± 10.87). Pregnancy rates were 18.04% in the microfluid group and 15.15% in the density gradient group, and ongoing PRs were 15.03% and 9.09%, respectively. After using multivariable logistic regression and controling for confounding factors, there was a significant increase in ongoing PRs in the microfluid sperm sorting group. The adjusted odds ratio for ongoing pregnancy in the microfluid group compared with the density gradient group was 3.49 (95% confidence interval 1.12-10.89)., Conclusion(s): The microfluid sperm sorting method significantly increased the ongoing PRs compared with the density gradient group in IUI cycles., (Copyright © 2019 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.)

The main obstacles on silver rasbora (Rasbora argyrotaenia) culture are having the limited number of broodstock and spawning depending on the season. The purpose of this study was to determine the effect of different dosage of Ovaprim™ induction by topical gill method to silver rasbora spermiation in order to continue the production out of its reproduction season with an optimum dose. A total of 30 male fish with a weight of 7.78 ± 0.20 g and length 4.11 ± 0.31 cm was used in this research. Topical gill treatments of Ovaprim™ were administered with following doses; 0.15 μl/g, 0.25 μl/g, 0.35 μl/g, 0.45 μl/g and 0.55 μl/g body weight. Milt volume, sperm concentration, sperm motility, and sperm viability parameters were observed in this study to understand the optimum dose of Ovaprim™ for male silver rasbora breeders. Spermiation induction of silver rasbora using Ovaprim™ with topical gill method has been successfully carried out, indicating an increase (P < 0.05) in milt volume, sperm concentration, sperm motility, and sperm viability. According to results a dose of Ovaprim™ is recommended to be used the 0.25 μl/g body weight in the spermiation induction of silver rasbora., (Published by Elsevier Inc.)

Purpose of Investigation: Gonadotropin stimulated intrauterine insemination (IUI) cycles performed following one month after hysterosalpingography (HSG) are associated with improvement in clinical pregnancy rates in unexplained infertile couples., Materials and Methods: A retrospective cohort study was performed between 2008 and 2014. A total of 92 unexplained infertile couples undergoing their first cycle IUI stimulated by gonadotropins were included in the analysis. Participants were classified into two groups according to IUI cycles performed one month (Group A, n = 25 cycles) or longer than one month (Group B, n = 67 cycles) after the HSG procedure., Result: The overall clinical pregnancy rate was found as 25% (23 clinical pregnancies / 92 cycles). Clinical pregnancy rate was 44 % (11/25) for Group A and 17.9 % (12/67) for Group B. In Group A, there were significantly higher clinical pregnancy rates compared to Group B (OR: 3.6, 95% CI, 1.3-9.8; p = 0.012)., Conclusions: It has been demonstrated that fertility improving effect of HSG was most prominent in the first six months after procedure. Likewise, in gonadotropin stimulated IUI cycles performed following one month after HSG, there seems to be an improvement in pregnancy rates in unexplained couples. In unexplained cases, it may be a reasonable approach to plan IUI cycles in the first month after HSG in clinical practice.

Objective: Repeated implantation failure (RIF) is a clinical entity affecting many couples undergoing assisted reproductive technology (ART). Various intrauterine pathologies contribute to RIF. Nevertheless, vaginal sonography and hysterosalpingography, which are the common diagnostic tools for the initial follow-up, have limited sensitivities. In this context, we aimed to evaluate the impact of office hysteroscopy (oHS) on live birth rates (LBRs) when performed prior to subsequent ART cycles in women with a history of RIF., Material and Methods: The database of an assisted reproduction center was retrospectively reviewed to detect eligible cases. A total of 363 women out of 2875 admissions were consecutively included in the analysis, of which 119 formed the oHS group and 244 formed the non-oHS group prior to a new ART cycle. Women in the oHS arm were examined during their early follicular phase via a vaginoscopic approach 1-6 months before the beginning of a new cycle. The standard in-vitro fertilization-intracytoplasmic sperm injection (IVF/ICSI) cycle was applied to all the women., Results: In the oHS group (n=119), 61 patients had intrauterine abnormalities, with an overall abnormality rate of 51.2%. Implantation, pregnancy, and LBRs of the groups were statistically similar. LBRs of the women with abnormal oHS findings (15/61, 24.5%), with normal oHS findings (14/58, 24.1%), and without oHS (39/244, 16%) were statistically similar (p=0.41)., Conclusion: Unrecognized intrauterine pathologies can be easily detected and concurrently treated during oHS with high success rate. However, a beneficial impact depends on the extent of the pathology and thus, routine application to enhance reproductive outcomes is still not warranted., Competing Interests: No conflict of interest was declared by the authors.

Pufferfish species of the Tetraodontidae family carry the smallest genomes among vertebrates. Their compressed genomes are thought to be enriched for functional DNA compared to larger vertebrate genomes, and they are important models for comparative genomics. The significance of pufferfish as model organisms in comparative genomics is due to the availability of two sequenced genomes, that of spotted green pufferfish (Tetraodon nigroviridis) and fugu (Takifugu rubripes). However, there is only a very limited utilization of pufferfish as an experimental model organism, due to the lack of established husbandry and developmental genetics protocols. In this study, we provide the first description of the normal embryonic development of Tetraodon nigroviridis. Embryos were obtained by in vitro fertilization of eggs, and subsequent development was monitored by brightfield microscopy at constant temperature. Tetraodon development was divided into distinct stages based on diagnostic morphological features, which were adopted from published literature on normal development of other fish species like medaka (Oryzias latipes), zebrafish (Danio rerio), and fugu. Tetraodon embryos show more similar morphologies to medaka than to zebrafish, reflecting its phylogenetic position. The early developmental stage series described in this study forms the foundation for the utilization of tetraodon as an experimental model organism for comparative developmental studies.

Objective: To evaluate whether oral contraceptive pill (OCP) therapy has any effects on ovarian stromal blood flow by using pulsed and color Doppler at the end of 3 months follow-up period of OCP-users and non-users with or without polycystic ovary syndrome (PCOS)., Study Design: 200 patients were included in the study. The patients were designed into four groups as follows; Group 1: PCOS patients that received OCP containing 30 mcg ethinyl estradiol (EE) plus 3mg drospirenone for 3 months (DRP n=50); Group 2: PCOS patients that received no medication (n=50); Group 3: Healthy controls that received OCP (EE plus DRP) (n=50); Group 4: healthy controls that received no medication (n=50). Resistance index (RI) and pulsatility index (PI) of both ovarian arteries, hormonal, anthropometric and biochemical parameters were assessed before and after 3 months., Results: There was a significant increament in RI and PI of both ovarian arteries in healthy controls (Group 3) and in women with PCOS (Group 1) who received OCP (p<0.001). The increment rate in both Doppler parameters were significantly higher in women with PCOS (Group 1) than healthy controls (Group 3) (p<0.001). Whereas RI and PI values of both ovaries remained unchanged in all untreated women with or without PCOS (Groups 2 and 4)., Conclusion: OCP therapy reduced ovarian vascularization in both PCOS and healthy users after 3 months of therapy and this decrease is especially noticeable in women with PCOS., (Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.)