Search

Your search keyword '"Bochev I"' showing total 23 results
Start Over Author "Bochev I"
23 results on '"Bochev I"'

1. Isolation of avian paramyxovirus type 1 (APMV-1) from free-living mallards (Anas platyrhynchos) (Preliminary communication)

Author

Zarkov, I., Bochev, I., Oreshkova, N., and Manvell, R.

Subjects
virus isolation, animal structures, Anas platyrhynchos, mallards, ducks, APMV-1
Abstract

n our attempts of virus isolation we examined 236 samples (faecal, cloacal, intestinal content, visceral organs). The samples were obtained from 16 species of wild, free-living birds from 06.12.2003 to 25.03.2004. They were collected from 5 different areas, situated in Southeastern Bulgaria.Viruses were isolated from the 3 consecutive passages, carried out on chicken embryos, in 5 samples (2.12% of the total number). All of them were from a single avian species (Anas platyrhynchos) and from one region.The isolates were defined by the haemagglutination test, followed by the haemagglutination inhibition test, as avian paramyxovirus type 1. In a consecutive study through RT-PCR carried out in the UK (Veterinary Laboratories Agency Weybridge, UK) they were related to the lentogenic representatives.

Published
2005

2. Andrology

Author

Carchenilla, M. S. C., primary, Agudo, D., additional, Rubio, S., additional, Becerra, D., additional, Bronet, F., additional, Garcia-Velasco, J. A., additional, Pacheco, A., additional, Lardone, M., additional, Piottante, A., additional, Parada-Bustamante, A., additional, Argandona, F., additional, Florez, M., additional, Espinoza, A., additional, Ebensperger, M., additional, Castro, A., additional, Cohen-Bacrie, M., additional, Belloc, S., additional, Dalleac, A., additional, Amar, E., additional, Izard, V., additional, Hazout, A., additional, Cohen-Bacrie, P., additional, de Mouzon, J., additional, Muzzonigro, F., additional, Crivello, A. M., additional, Stanghellini, I., additional, Bernardini, L., additional, Ferraretti, A. P., additional, Magli, C., additional, Gianaroli, L., additional, Martin, P. S., additional, Duvison, M. H., additional, Silva, M. D., additional, Gosalvez, J., additional, Martin, F. S., additional, Pomante, A., additional, Colombo, F., additional, Mattioli, M., additional, Barboni, B., additional, Magli, M. C., additional, Hacifazlioglu, O., additional, Findikli, N., additional, Goktolga, U., additional, Bahceci, M., additional, Jakab, A., additional, Mokanszki, A., additional, Varga, A., additional, Benyo, M., additional, Kassai, Z., additional, Olah, E., additional, Molnar, Z., additional, Gundogan, G. I., additional, Bozkurt, H. H., additional, Irez, T., additional, Domingo, A., additional, Anarte, C., additional, Presilla, N., additional, Calvo, I., additional, Aguirre, O., additional, Oroquieta, A., additional, Agirregoikoa, J. A., additional, De Pablo, J. L., additional, Barrenetxea, G., additional, Moragues, I., additional, Medrano, M. L., additional, Montoya, A., additional, Ramos, B., additional, Torres, M. J. G., additional, Aizpurua, J., additional, Ibala, S. R., additional, Ghedir, H., additional, Mehri, A., additional, Zidi, I., additional, Brahem, S., additional, Mehdi, M., additional, Ajina, M., additional, Saad, A., additional, Gomez-Torres, M. J., additional, Cavaco, J. E., additional, Rato, L., additional, Alves, M. G., additional, Dias, T. R., additional, Lopes, G., additional, Socorro, S., additional, Oliveira, P. F., additional, Lobascio, A. M., additional, Minasi, M. G., additional, Greco, E., additional, Bungum, M., additional, Bungum, A., additional, Silver, N., additional, Zahiri, M., additional, Movahedin, M., additional, Mowla, S. J., additional, Noruzinia, M., additional, Huleihel, M., additional, Abarbanel, Y., additional, Haber, E. P., additional, Azab, M., additional, Lan, D., additional, Lunenfeld, E., additional, Smith, M. J., additional, Neri, Q. V., additional, Harvey, L., additional, Rosenwaks, Z., additional, Palermo, G. D., additional, Alhalabi, M., additional, Samawi, S., additional, Droubi, H., additional, Khalaf, M., additional, Taha, A., additional, Khatib, R., additional, Bednarowska-flisiak, A., additional, Wcislo, M., additional, Liss, J., additional, Swider, A., additional, Szczyglinska, J., additional, Grzymkowska, M., additional, Bruszczynska, A., additional, Glowacka, J., additional, Kitowska-Marszalkowska, K., additional, Krapchev, M., additional, Mirecka, A., additional, Wisniewska, K., additional, Lukaszuk, K., additional, Natali, I., additional, Tamburrino, L., additional, Cambi, M., additional, Marchiani, S., additional, Noci, I., additional, Maggi, M., additional, Forti, G., additional, Baldi, E., additional, Muratori, M., additional, Ferraretto, X., additional, Pasquet, B., additional, Damond, F., additional, Matheron, S., additional, Epelboin, S., additional, Yahi, S., additional, Demailly, P., additional, Rougier, N., additional, Yazbeck, C., additional, Delaroche, L., additional, Longuet, P., additional, Llabador, M., additional, Estellat, C., additional, Patrat, C., additional, Askarijahromi, M., additional, Amanlu, M., additional, Mowla, S. j., additional, Mazaheri, Z., additional, Christensen, P., additional, Sills, E. S., additional, Fischer, R., additional, Naether, O. G. J., additional, Walsh, D., additional, Rudolf, K., additional, Coull, G., additional, Baukloh, V., additional, Labouriau, R., additional, Birck, A., additional, Parisi, F., additional, Parrilla, B., additional, Oneta, M., additional, Savasi, V., additional, Veleva, L., additional, Milachich, T., additional, Bochev, I., additional, Antonova, I., additional, Shterev, A., additional, Vlaisavljevic, V., additional, Breznik, B. P., additional, Kovacic, B., additional, Serrano, M., additional, Gonzalvo, M. C., additional, Clavero, A., additional, Fernandez, M. F., additional, Mozas, J., additional, Martinez, L., additional, Fontes, J., additional, Carrillo, S., additional, Lopez-Regalado, M. L., additional, Lopez-Leria, B., additional, Orozco, I., additional, Mantilla, A., additional, Castilla, J. A., additional, Mskhalaya, G., additional, Zakharova, E., additional, Zaletova, V., additional, Kasatonova, E., additional, Melnik, Y., additional, Efremov, E., additional, Schiewe, M. C., additional, Verheyen, G., additional, Tournaye, H., additional, Phletincx, I., additional, Sims, C. A., additional, Rothman, C., additional, Borges, E., additional, Setti, A. S., additional, Braga, D. P. A. F., additional, Vingris, L., additional, Iaconelli, A., additional, Dupont, C., additional, Faure, C., additional, Sermondade, N., additional, Gautier, B., additional, Herbemont, C., additional, Aknin, I., additional, Klein, J. P., additional, Cedrin-Durnerin, I., additional, Wolf, J. P., additional, Czernichow, S., additional, Levy, R., additional, Rondanino, C., additional, Chauffour, C., additional, Ouchchane, L., additional, Artonne, C., additional, Janny, L., additional, Lobaccaro, J. M., additional, Volle, D. H., additional, Brugnon, F., additional, Colacurci, N., additional, Piomboni, P., additional, Ruvolo, G., additional, Lombardo, F., additional, Verde, E. L., additional, De Leo, V., additional, Lispi, M., additional, Papaleo, E., additional, De Palo, R., additional, Gandini, L., additional, Longobardi, S., additional, Yokota, Y., additional, Yokota, M., additional, Yokota, H., additional, Araki, Y., additional, Alshahrani, S., additional, Durairajanayagam, D., additional, Sharma, R., additional, Sabanegh, E., additional, Agarwal, A., additional, Hattori, H., additional, Nakajo, Y., additional, Ikeno, T., additional, Sato, Y., additional, Kyoya, T., additional, Kyono, K., additional, Li, B., additional, Li, J. B., additional, Xiao, X. F., additional, Ma, Y. F., additional, Wang, J., additional, Liang, X. X., additional, Zhao, H. X., additional, Jiang, F., additional, Yao, Y. Q., additional, Wang, X. H., additional, Roan, N. R., additional, Liu, H., additional, Muller, J., additional, Avila-Herrera, A., additional, Pollard, K. S., additional, Lishko, P., additional, Kirchhoff, F., additional, Munch, J., additional, Witkowska, H. E., additional, Greene, W. C., additional, Mangiarini, A., additional, Paffoni, A., additional, Restelli, L., additional, Guarneri, C., additional, Somigliana, E., additional, Ragni, G., additional, Bou, R., additional, Aleman, M., additional, Guardiola, F., additional, Camargo, C., additional, Oliveira, J. B. A., additional, Petersen, C. G., additional, Mauri, A. L., additional, Massaro, F. C., additional, Nicoletti, A., additional, Nascimento, A. M., additional, Vagnini, L. D., additional, Martins, A. M. V. C., additional, Cavagna, M., additional, Baruffi, R. L. R., additional, and Franco, J. G., additional

Published
2013
Full Text
View/download PDF

3. EMBRYOLOGY

Author

Furia, G. U., primary, Kostelijk, E. H., additional, Vergouw, C. G., additional, Lee, H., additional, Lee, S., additional, Park, D., additional, Kang, H., additional, Lim, C., additional, Yang, K., additional, Park, Y., additional, Shin, M., additional, Beyhan, Z., additional, Fisch, J. D., additional, Sher, G., additional, Keskintepe, L., additional, VerMilyea, M. D., additional, Anthony, J. T., additional, Graham, J. R., additional, Tucker, M. J., additional, Freour, T., additional, Lattes, S., additional, Lammers, J., additional, Mansour, W., additional, Jean, M., additional, Barriere, P., additional, El Danasouri, I., additional, Gagsteiger, F., additional, Rinaldi, L., additional, Selman, H., additional, Antonova, I., additional, Milachich, T., additional, Valkova, L., additional, Shterev, A., additional, Barcroft, J., additional, Dayoub, N., additional, Thong, J., additional, Abdel Reda, H., additional, Khalaf, Y., additional, El Touky, T., additional, Cabry, R., additional, Brzakowski, R., additional, Lourdel, E., additional, Brasseur, F., additional, Copin, H., additional, Merviel, P., additional, Yamada, M., additional, Takanashi, K., additional, Hamatani, T., additional, Akutsu, H., additional, Fukunaga, T., additional, Inoue, O., additional, Ogawa, S., additional, Sugawara, K., additional, Okumura, N., additional, Chikazawa, N., additional, Kuji, N., additional, Umezawa, A., additional, Tomita, M., additional, Yoshimura, Y., additional, Van der Jeught, M., additional, Ghimire, S., additional, O'Leary, T., additional, Lierman, S., additional, Deforce, D., additional, Chuva de Sousa Lopes, S., additional, Heindryckx, B., additional, De Sutter, P., additional, Herrero, J., additional, Tejera, A., additional, De los Santos, M. J., additional, Castello, D., additional, Romero, J. L., additional, Meseguer, M., additional, Leperlier, F., additional, Mirallie, S., additional, Schats, R., additional, Al-Nofal, M., additional, Lens, J. W., additional, Rooth, H., additional, Hompes, P. G., additional, Lambalk, C. B., additional, Hreinsson, J., additional, Karlstrom, P. O., additional, Wanggren, K., additional, Lundqvist, M., additional, Vahabi, Z., additional, Eftekhari-Yazdi, P., additional, Dalman, A., additional, Ebrahimi, B., additional, Daneshzadeh, M. T., additional, Rajabpour Niknam, M., additional, Choi, E. G., additional, Rho, Y. H., additional, Oh, D. S., additional, Park, L. S., additional, Cheon, H. S., additional, Lee, C. S., additional, Kong, I. K., additional, Lee, S. C., additional, Liebenthron, J., additional, Montag, M., additional, Koster, M., additional, Toth, B., additional, Reinsberg, J., additional, van der Ven, H., additional, Strowitzki, T., additional, Morita, H., additional, Hirosawa, T., additional, Watanabe, S., additional, Wada, T., additional, Kamihata, M., additional, Kuwahata, A., additional, Ochi, M., additional, Horiuchi, T., additional, Fatemeh, H., additional, Karimian, L., additional, Fazel, M., additional, Fouladi, H., additional, Johansson, L., additional, Ruttanajit, T., additional, Chanchamroen, S., additional, Sopaboon, P., additional, Seweewanlop, S., additional, Sawakwongpra, K., additional, Jindasri, P., additional, Jantanalapruek, T., additional, Charoonchip, K., additional, Vajta, G., additional, Quangkananurug, W., additional, Yi, G., additional, Jo, J. W., additional, Jee, B. C., additional, Suh, C. S., additional, Kim, S. H., additional, Zhang, Y., additional, Zhao, H. J., additional, Cui, Y. G., additional, Gao, C., additional, Gao, L. L., additional, Liu, J. Y., additional, Sozen, E., additional, Buluc, B., additional, Vicdan, K., additional, Akarsu, C., additional, Tuncay, G., additional, Hambiliki, F., additional, Bungum, M., additional, Agapitou, K., additional, Makrakis, E., additional, Liarmakopoulou, S., additional, Anagnostopoulou, C., additional, Moustakarias, T., additional, Giannaris, D., additional, Wang, J., additional, Andonov, M., additional, Linara, E., additional, Charleson, C., additional, Ahuja, K. K., additional, Ozsoy, S., additional, Morris, M. B., additional, Day, M. L., additional, Cobo, A., additional, Viloria, T., additional, Campos, P., additional, Vallejo, B., additional, Remohi, J., additional, Roldan, M., additional, Perez-Cano, I., additional, Cruz, M., additional, Martinez, M., additional, Gadea, B., additional, Munoz, M., additional, Garrido, N., additional, Mesut, N., additional, Ciray, H. N., additional, Mesut, A., additional, Isler, A., additional, Bahceci, M., additional, Fortuno, S., additional, Legidos, V., additional, Muela, L., additional, Galindo, N., additional, Gunasheela, S., additional, Gunasheela, D., additional, Ueno, S., additional, Uchiyama, K., additional, Kondo, M., additional, Ito, M., additional, Kato, K., additional, Takehara, Y., additional, Kato, O., additional, Edgar, D. H., additional, Krapez, J. A., additional, Bacer Kermavner, L., additional, Virant-Klun, I., additional, Pinter, B., additional, Tomazevic, T., additional, Vrtacnik-Bokal, E., additional, Lee, S. G., additional, Kang, S. M., additional, Lee, S. W., additional, Jeong, H. J., additional, Lee, Y. C., additional, Lim, J. H., additional, Bochev, I., additional, Kyurkchiev, S., additional, Wilding, M., additional, Coppola, G., additional, Di Matteo, L., additional, Dale, B., additional, Hormann-Kropfl, M., additional, Kastelic, D., additional, Schenk, M., additional, Fourati Ben Mustapha, S., additional, Khrouf, M., additional, Braham, M., additional, Kallel, L., additional, Elloumi, H., additional, Merdassi, G., additional, Chaker, A., additional, Ben Meftah, M., additional, Zhioua, F., additional, Zhioua, A., additional, Kocent, J., additional, Neri, Q. V., additional, Rosenwaks, Z., additional, Palermo, G. D., additional, Best, L., additional, Campbell, A., additional, Fishel, S., additional, Calimlioglu, N., additional, Sahin, G., additional, Akdogan, A., additional, Susamci, T., additional, Bilgin, M., additional, Goker, E. N. T., additional, Tavmergen, E., additional, Cantatore, C., additional, Ding, J., additional, Depalo, R., additional, Smith, G. D., additional, Kasapi, E., additional, Panagiotidis, Y., additional, Papatheodorou, A., additional, Goudakou, M., additional, Pasadaki, T., additional, Nikolettos, N., additional, Asimakopoulos, B., additional, Prapas, Y., additional, Soydan, E., additional, Gulebenzer, G., additional, Karatekelioglu, E., additional, Budak, E., additional, Pehlivan Budak, T., additional, Alegretti, J., additional, Cuzzi, J., additional, Negrao, P. M., additional, Moraes, M. P., additional, Bueno, M. B., additional, Serafini, P., additional, Motta, E. L. A., additional, Elaimi, A., additional, Harper, J. C., additional, Stecher, A., additional, Baborova, P., additional, Wirleitner, B., additional, Schwerda, D., additional, Vanderzwalmen, P., additional, Zech, N. H., additional, Stanic, P., additional, Hlavati, V., additional, Gelo, N., additional, Pavicic-Baldani, D., additional, Sprem-Goldstajn, M., additional, Radakovic, B., additional, Kasum, M., additional, Strelec, M., additional, Simunic, V., additional, Vrcic, H., additional, Khan, I., additional, Urich, M., additional, Abozaid, T., additional, Ullah, K., additional, Abuzeid, M., additional, Fakih, M., additional, Shamma, N., additional, Ayers, J., additional, Ashraf, M., additional, Milik, S., additional, Pirkevi, C., additional, Atayurt, Z., additional, Yazici, S., additional, Yelke, H., additional, Kahraman, S., additional, Dal Canto, M., additional, Coticchio, G., additional, Brambillasca, F., additional, Mignini Renzini, M., additional, Novara, P., additional, Maragno, L., additional, Karagouga, G., additional, De Ponti, E., additional, Fadini, R., additional, Resta, S., additional, Magli, M. C., additional, Cavallini, G., additional, Muzzonigro, F., additional, Ferraretti, A. P., additional, Gianaroli, L., additional, Barberi, M., additional, Orlando, G., additional, Sciajno, R., additional, Serrao, L., additional, Fava, L., additional, Preti, S., additional, Bonu, M. A., additional, Borini, A., additional, Varras, M., additional, Polonifi, A., additional, Mantzourani, M., additional, Mavrogianni, D., additional, Stefanidis, K., additional, Griva, T., additional, Bletsa, R., additional, Dinopoulou, V., additional, Drakakis, P., additional, Loutradis, D., additional, Hickman, C. F. L., additional, Duffy, S., additional, Bowman, N., additional, Gardner, K., additional, Sati, L., additional, Zeiss, C., additional, Demir, R., additional, McGrath, J., additional, Yildiz, S., additional, Unal, S., additional, Kumtepe, Y., additional, Aljaser, F., additional, Hernandez, J., additional, Tomlinson, M., additional, Campbell, B., additional, Fosas, N., additional, Redondo Ania, M., additional, Marina, F., additional, Molfino, F., additional, Martin, P., additional, Perez, N., additional, Carrasco, A., additional, Garcia, N., additional, Gonzalez, S., additional, Marina, S., additional, Scaruffi, P., additional, Stigliani, S., additional, Tonini, G. P., additional, Venturini, P. L., additional, Anserini, P., additional, Guglielmo, M. C., additional, Albertini, D. F., additional, Lain, M., additional, Caliari, I., additional, Oikonomou, Z., additional, Chatzimeletiou, K., additional, Sioga, A., additional, Oikonomou, L., additional, Kolibianakis, E., additional, Tarlatzis, B., additional, Nottola, S. A., additional, Bianchi, V., additional, Lorenzo, C., additional, Maione, M., additional, Macchiarelli, G., additional, Gomez, E., additional, Gil, M. A., additional, Sanchez-Osorio, J., additional, Maside, C., additional, Martinez, M. J., additional, Torres, I., additional, Rodenas, C., additional, Cuello, C., additional, Parrilla, I., additional, Molina, G., additional, Garcia, A., additional, Margineda, J., additional, Navarro, S., additional, Roca, J., additional, Martinez, E. A., additional, Avcil, F., additional, Ozden, H., additional, Candan, Z. N., additional, Uslu, H., additional, Karaman, Y., additional, Gioacchini, G., additional, Giorgini, E., additional, Carnevali, O., additional, Ferraris, P., additional, Vaccari, L., additional, Choe, S., additional, Tae, J., additional, Kim, C., additional, Lee, J., additional, Hwang, D., additional, Kim, K., additional, Suh, C., additional, Jee, B., additional, Catt, S. L., additional, Sorenson, H., additional, Vela, M., additional, Duric, V., additional, Chen, P., additional, Temple-Smith, P. D., additional, Pangestu, M., additional, Yoshimura, T., additional, Fukunaga, N., additional, Nagai, R., additional, Kitasaka, H., additional, Tamura, F., additional, Hasegawa, N., additional, Kato, M., additional, Nakayama, K., additional, Takeuchi, M., additional, Aoyagi, N., additional, Yasue, K., additional, Watanabe, H., additional, Asano, E., additional, Hashiba, Y., additional, Asada, Y., additional, Iwata, K., additional, Yumoto, K., additional, Mizoguchi, C., additional, Sargent, H., additional, Kai, Y., additional, Ueda, M., additional, Tsuchie, Y., additional, Imajo, A., additional, Iba, Y., additional, Mio, Y., additional, Els-Smit, C. L., additional, Botha, M. H., additional, Sousa, M., additional, Windt-De Beer, M., additional, Kruger, T. F., additional, Muller, N., additional, Magli, C., additional, Corani, G., additional, Giusti, A., additional, Castelletti, E., additional, Gambardella, L., additional, Seshadri, S., additional, Sunkara, S. K., additional, El-Toukhy, T., additional, Kishi, I., additional, Maruyama, T., additional, Ohishi, M., additional, Akiba, Y., additional, Asada, H., additional, Konishi, Y., additional, Nakano, M., additional, Kamei, K., additional, Lee, J. H., additional, Lee, K. H., additional, Park, I. H., additional, Sun, H. G., additional, Kim, S. G., additional, Kim, Y. Y., additional, Choi, E. M., additional, Lee, D. H., additional, Chavez, S. L., additional, Loewke, K. E., additional, Behr, B., additional, Han, J., additional, Moussavi, F., additional, Reijo Pera, R. A., additional, Yokota, H., additional, Yokota, Y., additional, Yokota, M., additional, Sato, S., additional, Nakagawa, M., additional, Sato, M., additional, Anazawa, I., additional, Araki, Y., additional, Knez, K., additional, Pozlep, B., additional, Vermilyea, M. D., additional, Levy, M. J., additional, Carvalho, M., additional, Cordeiro, I., additional, Leal, F., additional, Aguiar, A., additional, Nunes, J., additional, Rodrigues, C., additional, Soares, A. P., additional, Sousa, S., additional, Calhaz-Jorge, C., additional, Braga, D. P. A. F., additional, Setti, A. S., additional, Figueira, R. C. S., additional, Aoki, T., additional, Iaconelli, A., additional, Borges, E., additional, Ozkavukcu, S., additional, Sonmezer, M., additional, Atabekoglu, C., additional, Berker, B., additional, Ozmen, B., additional, Isbacar, S., additional, Ibis, E., additional, Menezes, J., additional, Lalitkumar, P. G. L., additional, Borg, P., additional, Ekwurtzel, E., additional, Nordqvist, S., additional, Vaegter, K., additional, Tristen, C., additional, Sjoblom, P., additional, Azevedo, M. C., additional, Remohi Gimenez, J., additional, Gamiz, P., additional, Albert, C., additional, Ferreira, R. C., additional, Resende, S., additional, Colturato, S. S., additional, Ferrer Buitrago, M., additional, Ferrer Robles, E., additional, Munoz Soriano, P., additional, Ruiz-Jorro, M., additional, Calatayud Lliso, C., additional, Rawe, V. Y., additional, Hanrieder, J., additional, Gulen-Yaldir, F., additional, Bergquist, J., additional, Stavreus-Evers, A., additional, Grunskis, A., additional, Bazarova, A., additional, Dundure, I., additional, Fodina, V., additional, Brikune, J., additional, Lakutins, J., additional, Pribenszky, C., additional, Cornea, M., additional, Reichart, A., additional, Uhereczky, G., additional, Losonczy, E., additional, Ficsor, L., additional, Lang, Z., additional, Ohgi, S., additional, Nakamura, C., additional, Hagiwara, C., additional, Kawashima, M., additional, Yanaihara, A., additional, Jones, G. M., additional, Biba, M., additional, Kokkali, G., additional, Vaxevanoglou, T., additional, Chronopoulou, M., additional, Petroutsou, K., additional, Sfakianoudis, K., additional, Pantos, K., additional, Romano, S., additional, Albricci, L., additional, Stoppa, M., additional, Cerza, C., additional, Sanges, F., additional, Fusco, S., additional, Capalbo, A., additional, Maggiulli, R., additional, Ubaldi, F., additional, Rienzi, L., additional, Ulrick, J., additional, Kilani, S., additional, Chapman, M., additional, Losada, C., additional, Ortega, I., additional, Pacheco, A., additional, Bronet, F., additional, Aguilar, J., additional, Ojeda, M., additional, Taboas, E., additional, Perez, M., additional, Munoz, E., additional, Pellicer, A., additional, Boumela, I., additional, Assou, S., additional, Haouzi, D., additional, Monzo, C., additional, Dechaud, H., additional, Hamamah, S., additional, Nakaoka, Y., additional, Hashimoto, S., additional, Amo, A., additional, Yamagata, K., additional, Nakano, T., additional, Akamatsu, Y., additional, Mezawa, T., additional, Ohnishi, Y., additional, Himeno, T., additional, Inoue, T., additional, Ito, K., additional, and Morimoto, Y., additional

Published
2012
Full Text
View/download PDF

4. POSTER VIEWING SESSION - ANDROLOGY

Author

Dul, E. C., primary, van Ravenswaaij-Arts, C. M. A., additional, Groen, H., additional, van Echten-Arends, J., additional, Land, J. A., additional, Tyulenev, Y., additional, Naumenko, V., additional, Kurilo, L., additional, Shileiko, L., additional, Segal, A., additional, Klimova, R., additional, Kushch, A., additional, Ribas-Maynou, J., additional, Garcia-Peiro, A., additional, Abad, C., additional, Amengual, M. J., additional, Benet, J., additional, Navarro, J., additional, Colasante, A., additional, Lobascio, A. M., additional, Scarselli, F., additional, Minasi, M. G., additional, Alviggi, E., additional, Rubino, P., additional, Casciani, V., additional, Pena, R., additional, Varricchio, M. T., additional, Litwicka, K., additional, Ferrero, S., additional, Zavaglia, D., additional, Franco, G., additional, Nagy, Z. P., additional, Greco, E., additional, Romany, L., additional, Meseguer, M., additional, Garcia-Herrero, S., additional, Pellicer, A., additional, Garrido, N., additional, Dam, A., additional, Pijnenburg, A., additional, Hendriks, J. C., additional, Westphal, J. R., additional, Ramos, L., additional, Kremer, J. A. M., additional, Eertmans, F., additional, Bogaert, V., additional, Puype, B., additional, Geisler, W., additional, Clusmann, C., additional, Klopsch, I., additional, Strowitzki, T., additional, Eggert-Kruse, W., additional, Maettner, R., additional, Isachenko, E., additional, Isachenko, V., additional, Strehler, E., additional, Sterzik, K., additional, Band, G., additional, Madgar, I., additional, Brietbart, H., additional, Naor, Z., additional, Cunha-Filho, J. S., additional, Souza, C. A., additional, Krebs, V. G., additional, Santos, K. D., additional, Koff, W. J., additional, Stein, A., additional, Hammoud, I., additional, Albert, M., additional, Bergere, M., additional, Bailly, M., additional, Boitrelle, F., additional, Vialard, F., additional, Wainer, R., additional, Izard, V., additional, Selva, J., additional, Cohen - Bacrie, P., additional, Belloc, S., additional, de mouzon, J., additional, Cohen-Bacrie, M., additional, Alvarez, S., additional, Junca, A. M., additional, Dumont, M., additional, Douard, S., additional, Prisant, N., additional, Tomita, K., additional, Hashimoto, S., additional, Akamatsu, Y., additional, Satoh, M., additional, Mori, R., additional, Inoue, T., additional, Ohnishi, Y., additional, Ito, K., additional, Nakaoka, Y., additional, Morimoto, Y., additional, Smith, V. J. H., additional, Ahuja, K. K., additional, Atig, F., additional, Raffa, M., additional, Sfar, M. T., additional, Saad, A., additional, Ajina, M., additional, Braga, D. P. A. F., additional, Halpern, G., additional, Figueira, R. C. S., additional, Setti, A. S., additional, Iaconelli Jr., A., additional, Borges Jr., E., additional, Medeiros, G. S., additional, Pasqualotto, E. B., additional, Pasqualotto, F. F., additional, Nadalini, M., additional, Tarozzi, N., additional, Di Santo, M., additional, Borini, A., additional, Lopez-Fernandez, C., additional, Arroyo, F., additional, Caballero, P., additional, Nunez-Calonge, R., additional, Fernandez, J. L., additional, Gosalvez, J., additional, Gosalbez, A., additional, Cortes, S., additional, Zikopoulos, K., additional, Lazaros, L., additional, Vartholomatos, G., additional, Kaponis, A., additional, Makrydimas, G., additional, Plachouras, N., additional, Sofikitis, N., additional, Kalantaridou, S., additional, Hatzi, E., additional, Georgiou, I., additional, de Mouzon, J., additional, Amar, E., additional, Cohen-Bacrie, P., additional, Vuillaume, M. L., additional, Brugnon, F., additional, Artonne, C., additional, Janny, L., additional, Pons-Rejraji, H., additional, Fedder, J., additional, Bosco, L., additional, Ruvolo, G., additional, Bruccoleri, A. M., additional, Manno, M., additional, Roccheri, M. C., additional, Cittadini, E., additional, Bochev, I., additional, Gavrilov, P., additional, Kyurkchiev, S., additional, Shterev, A., additional, Carlomagno, G., additional, Colone, M., additional, Condorelli, R. A., additional, Stringaro, A., additional, Calogero, A. E., additional, Zakova, J., additional, Kralikova, M., additional, Crha, I., additional, Ventruba, P., additional, Melounova, J., additional, Matejovicova, M., additional, Vodova, M., additional, Lousova, E., additional, Sanchez Toledo, M., additional, Alvarez LLeo, C., additional, Garcia Garrido, C., additional, Resta Serra, M., additional, Belmonte Andujar, L. L., additional, Gonzalez de Merlo, G., additional, Pohanka, M., additional, Huser, M., additional, Amiri, I., additional, Karimi, J., additional, Goodarzi, M. T., additional, Tavilani, H., additional, Filannino, A., additional, Magli, M. C., additional, Boudjema, E., additional, Crippa, A., additional, Ferraretti, A. P., additional, Gianaroli, L., additional, Robles, F., additional, Huang, H., additional, Yao, D. J., additional, Huang, H. J., additional, Li, J. R., additional, Fan, S. K., additional, Wang, M. L., additional, Yung-Kuei, S., additional, Amer, S., additional, Mahran, A., additional, Darne, J., additional, Shaw, R., additional, Borghi, E., additional, Cetera, C., additional, Shukla, U., additional, Ogutu, D., additional, Deval, B., additional, Jansa, M., additional, Savvas, M., additional, Narvekar, N., additional, Houska, P., additional, Dackland, A. L., additional, Bjorndahl, L., additional, Kvist, U., additional, Muzii, L., additional, Barboni, B., additional, Samanta, L., additional, Kar, S., additional, Yakovenko, S. A., additional, Troshina, M. N., additional, Rutman, B. K., additional, Dyakonov, S. A., additional, Holmes, E., additional, Feijo, C., additional, Verza Junior, S., additional, Esteves, S. C., additional, Berta, C. L., additional, Caille, A. M., additional, Ghersevich, S. A., additional, Zumoffen, C., additional, Munuce, M. J., additional, San Celestino, M., additional, Agudo, D., additional, Alonso, M., additional, Sanjurjo, P., additional, Becerra, D., additional, Bronet, F., additional, Garcia-Velasco, J. A., additional, Pacheco, A., additional, Lafuente, R., additional, Lopez, G., additional, Checa, M. A., additional, Carreras, R., additional, Brassesco, M., additional, Oneta, M., additional, Savasi, V., additional, Parrilla, B., additional, Guarneri, D., additional, Laureti, A., additional, Pagano, F., additional, Cetin, I., additional, Ekwurtzel, E., additional, Morgante, G., additional, Piomboni, P., additional, Stendardi, A., additional, Serafini, F., additional, De Leo, V., additional, Focarelli, R., additional, Benkhalifa, M., additional, De Mouzon, J., additional, Entezami, F., additional, Junca, A., additional, De Mouzon, J. J., additional, Mangiarini, A., additional, Capitanio, E., additional, Paffoni, A., additional, Restelli, L., additional, Guarneri, C., additional, Scarduelli, C., additional, Ragni, G., additional, Harrison, K., additional, Irving, J., additional, Martin, N., additional, Sherrin, D., additional, Yazdani, A., additional, Almeida, C., additional, Correia, S., additional, Rocha, E., additional, Alves, A., additional, Cunha, M., additional, Ferraz, L., additional, Silva, S., additional, Sousa, M., additional, Barros, A., additional, Perdrix, A., additional, Travers, A., additional, Milazzo, J. P., additional, Clatot, F., additional, Mousset-Simeon, N., additional, Mace, B., additional, Rives, N., additional, Clarke, H. S., additional, Callow, A., additional, Saxton, D., additional, Pacey, A. A., additional, Sapir, O., additional, Oron, G., additional, Ben-Haroush, A., additional, Garor, R., additional, Feldberg, D., additional, Pinkas, H., additional, Wertheimer, A., additional, Fisch, B., additional, Palacios, E., additional, Gonzalvo, M. C., additional, Clavero, A., additional, Ramirez, J. P., additional, Rosales, A., additional, Mozas, J., additional, Castilla, J. A., additional, Mugica, J., additional, Ramon, O., additional, Valdivia, A., additional, Exposito, A., additional, Casis, L., additional, Matorras, R., additional, Bongers, R., additional, Gottardo, F., additional, Zitzmann, M., additional, Kliesch, S., additional, Cordes, T., additional, Kamischke, A., additional, Schultze-Mosgau, A., additional, Buendgen, N., additional, Diedrich, K., additional, Griesinger, G., additional, Crisol, L., additional, Aspichueta, F., additional, Hernandez, M. L., additional, Ruiz-Sanz, J. I., additional, Mendoza, R., additional, Sanchez-Tusie, A. A., additional, Bermudez, A., additional, Lopez, P., additional, Churchill, G. C., additional, Trevino, C. L., additional, Maldonado, I., additional, and Dabbah, J., additional

Published
2011
Full Text
View/download PDF

6. COMPARISON OF THE SEROPREVALENCE AGAINST SOME RESPIRATORY VIRUSES IN MIXED SHEEP-GOAT HERDS IN TWO REGIONS OF BULGARIA.

Author

Rusenova, N. and Bochev, I.

Subjects
*SEROPREVALENCE, *BOVINE viral diarrhea virus, *PARAINFLUENZA viruses, *HERPESVIRUSES, *ADENOVIRUSES, *IMMUNOGLOBULINS, *SHEEP, *GOATS
Abstract

In the course of the study 199 blood samples from sheep and goats were tested. They were collected from 4 mixed herds, situated in 4 different settlements, divided in two regions (two settlements in each). The sera were tested for presence of virus-neutralizing antibodies against Parainfluenza-3 Virus (PI-3), Bovine Viral Diarrhea Virus (BVDV) and Bovine Herpesvirus -1 (BHV-1); they were also tested for groupspecific antibodies against adenoviruses through Ovine adenovirus serotype 3 (OAV-3). For detection of virus-neutralizing antibodies a micro-virusneutralizing reaction (MVNR) was used. For detection of group-specific antibodies immuno -diffusion test (ID) was used. The average seroprevalence among sheep was 2.4% to BHV-1; 64 % to BVDV, 31.3 % to PI-3 and 37.3% to OAV-3. Among goats average seroprevalence was 3.4 % to BHV-1; 18.1 % to BVDV, 58.6 % to PI-3 and 69 % to OAV-3. [ABSTRACT FROM AUTHOR]

Published
2009

7. PORCINE RESPIRATORY DISEASE COMPLEX (PRDC): A REVIEW. II. DIAGNOSTICS, TREATMENT AND PREVENTION.

Author

BOCHEV, I.

Subjects
*SWINE diseases, *TUBERCULOSIS, *RESPIRATORY diseases, *ENZYME-linked immunosorbent assay, *IMMUNOENZYME technique, *SOLID-phase analysis, *SWINE
Abstract

The diagnostics of porcine respiratory disease complex (PRDC) requires a complex investigation with compulsory use of a serological assay (enzyme-linked immunosorbent assay, ELISA) The polymerase chain reaction (PCR) assay is also becoming more and more important. Prevention plays an essential role for eradication of this disease complex. The treatment with antibiotics is less important. The control measures should be complex and are mainly focused on restriction and hygiene. They include parent herds' closure, schedules for early weaning of piglets (segregated or medicated early weaning), the all-in/all-out management technology, periodical serological surveys etc. Vaccinations result in relatively weak effect against the main PRDC agents. The use of live vaccines in PRRSV interferes with the diagnostics and generates a risk of virulentization of the vaccinal strain whereas killed vaccines are not sufficiently immunogenic and therefore also impede diagnostics. The weak immunity in Mycoplasma infections is mainly due to the epithelial localization of pathogens, that makes them hardly accessible for host immune defense. The non-specific prevention is also prevailing for control of other bacterial agents of PRDC. [ABSTRACT FROM AUTHOR]

Published
2008

13. Assisted oocyte activation significantly improves zygote formation, cleavage, and implantation rates in patients with a history of fertilization failures.

Author

Antonova I, Yunakova M, Bochev I, Magunska N, Yaneva G, and Ivanov D

Subjects
Humans, Female, Adult, Retrospective Studies, Pregnancy, Pregnancy Rate, Fertilization physiology, Fertilization drug effects, Fertilization in Vitro methods, Treatment Failure, Embryo Transfer methods, Male, Zygote physiology, Sperm Injections, Intracytoplasmic methods, Oocytes physiology, Oocytes drug effects, Calcium Ionophores pharmacology, Embryo Implantation physiology
Abstract

Background: Fertilization check performed at the 18th hour following classic in vitro fertilization procedure (IVF) or intracytoplasmic sperm injection (ICSI) is a critical stage in assisted reproduction. The success of the treatment is significantly reliant on the quantity of zygotes exhibiting two pronuclei. Consequently, low fertilization rates or complete fertilization failure are highly undesirable outcomes for both patients and reproductive specialists. Applying additional calcium ionophore for oocyte activation subsequent to ICSI may offer benefits and potentially enhance treatment outcomes, particularly for patients who have experienced low or absent fertilization rates (FR) in previous treatment cycles. The aim of the study is to evaluate the efficacy of Ca2+ ionophore application for oocyte activation., Methods: A retrospective analysis of 924 oocytes obtained from 120 patients who underwent ICSI cycles with a history of low or no fertilization as a result of previous unsuccessful treatment rounds. The next ART cycle followed with additional oocyte Ca2+ ionophore activation applied in 57 of the cases in order to optimize the treatment process (Group 1), and 63 patients were included and their outcomes followed as a control group (Group 2).We conducted a comparative analysis of results in both groups. The study's primary outcomes encompassed fertilization, cleavage embryo quality, blastocyst rate, and established clinical pregnancies., Results: At day 1 fertilization check we had 274/386 zygotes (71%FR) in group 1 and 132/410 in group 2 (32.2%FR), (P < 0.0001). Twenty-two (34.9%) cycles in group 2 resulted in total fertilization failure (TFF). At the cleavage stage top-quality embryos from group 1 were significantly higher (P = 0.0021) in comparison to group 2. Forty-eight embryo transfers (ET) were performed in group 1 resulting in 41.67% clinical pregnancies versus 33 ET and only 4 pregnancies (12.12%) for group 2 (P = 0.0044)., Conclusions: The results confirm the appropriateness of assisted oocyte activation as an additional method in cases of previous fertilization failure cycles.

Published
2024
Full Text
View/download PDF

14. Potential role of biochemical placentation markers - pregnancy associated plasma protein-A and human chorionic gonadotropin for early gestational diabetes screening - a pilot study.

Author

Yanachkova VE, Staynova R, Bochev I, and Kamenov Z

Abstract

Objectives: Gestational diabetes mellitus (GDM) is one of the most common pregnancy complications. The universal screening for GDM is usually performed between 24-28 weeks' gestation. This often delays the diagnosis and could increase the risk of adverse pregnancy outcomes. Some of the biochemical placental markers - pregnancy associated plasma protein A (PAPP-A) and free-β human chorionic gonadotropin (hCG), probably could provide a diagnostic value for GDM. The aim of our study was to assess if PAPP-A and hCG values were different among pregnant women with and without GDM and respectively, to tested their place in the early GDM screening., Material and Methods: We conducted a retrospective, case-control study by reviewing the clinical database records of 662 pregnant women. The analysis includes the data for a two-year period. The patients included in the observation were divided into two groups - GDM group (n = 412) and Euglycemic group (n = 250). Early screening for GDМ between 9-12 weeks' gestation was performed in 173 of the women in the interventional group due to: registered fasting plasma glucose (FPG) above 5.1 mmol/L, obesity, macrosomia in previous pregnancies or family history for diabetes mellitus. The remaining 239 women underwent universal screening at 24-28 weeks' gestation. Mean serum levels of PAPP-A, hCG, FPG, and body mass index (BMI) were measured between 10-13 gestational weeks. Serum levels of PAPP-A and hCG are presented as multiples of the normal median (MoM), adjusted by maternal baseline characteristics and demographics., Results: In patients who developed GDM during pregnancy, compared with the control group, we have found significantly lower MoM values of PAPP-A (p < 0.0001), higher levels of FPG (р < 0.0001) and higher BMI (р < 0.0001). Median hCG MoM was similar in both group of pregnant women., Conclusion: Our findings suggest that low-normal to low reference range values of PAPP-A might be associated with higher risk for GDM. PAAP-A levels can be used as an additional factor to recommend early screening for GDM.

Published
2021
Full Text
View/download PDF

15. A study of the transformation of umbilical cord mesenchymal stem cells by interferon-gamma.

Author

Belemezova K, Bochev I, Ivanova-Todorova E, Kyurkchiev S, and Kyurkchiev D

Abstract

Objectives: Mesenchymal stem cells (MSCs) exist in almost all tissues. Their unique nature is completed by their immunomodulatory functions, holding promise for the treatment of many diseases. An inflammatory environment precedes the immunosuppressive abilities of MSCs and this study was intended to better understand how umbilical cord MSCs (UCMSCs) react to the process of inflammation, regarding their basic characteristics and behavior when primed with the key pro-inflammatory cytokine, Interferon-γ (IFNγ)., Materials and Methods: Human MSCs from the umbilical cord were isolated, expanded, and treated with IFNγ. Primed cells were analyzed to define their ability to form colonies, their morphology, differentiation potential, proliferation, and apoptosis rate., Results: UCMSCs treated with IFNγ changed their fibroblast-like morphology and retained the expression of typical MSCs markers. IFNγ treated UCMSCs had significantly higher MFI levels regarding the expression of HLA-I (980.43 ± 556.64) and PD-L1 (598.04 ± 416.90) compared with the control cells (144.97 ± 78.5 and 122.05 ± 103.83, respectively; P <0.01). Under the influence of IFNγ, the cells had a lower population doubling time compared with the control cultures (50.345 ± 9.155 versus 61.135 ± 21.110, respectively; P <0.01) and higher numbers of colony-forming unit-fibroblasts (26.0 ± 12.2 versus 10.2 ± 8.0, respectively; P <0.05). The primed MSCs could not undergo osteogenic and adipogenic differentiation. IFNγ increased the percentage of cells in the apoptotic state on day eight (29.470 ± 6.59 versus 15.708 ± 6.190, respectively; P <0.01)., Conclusion: The properties of UCMSCs can be influenced by the pro-inflammatory cytokine IFNγ., Competing Interests: The authors declare that they have no conflicts of interest.

Published
2021
Full Text
View/download PDF

16. Effect of cryopreservation on the properties of human endometrial stromal cells used in embryo co-culture systems.

Author

Bochev I, Belemezova K, Shterev A, and Kyurkchiev S

Subjects
Endometrium cytology, Endometrium metabolism, Female, Humans, Insulin-Like Growth Factor Binding Protein 1 metabolism, Interleukin-6 metabolism, Pregnancy, Pregnancy Rate, Prolactin metabolism, Stromal Cells cytology, Stromal Cells metabolism, Coculture Techniques methods, Cryopreservation, Fertilization in Vitro, Reproductive Techniques, Assisted
Abstract

Purpose: Along with comparative investigation of the decidualization potential and IL-6 secretion by fresh and frozen ESCs, we also aimed to evaluate the effectiveness of co-culture systems based on fresh or frozen ESCs in terms of clinical pregnancy rates., Methods: Outcome analysis of a total of 215 IVF cycles with co-culture with fresh or frozen ESCs was performed. Endometrial tissue was obtained from 17 healthy donors. Concentrations of secreted prolactin, IGFBP-1, and IL-6 in conditioned media from cultured fresh and frozen ESCs (decidualized or not) were measured using ELISA or ECLIA., Results: Embryo co-culture with frozen ESCs resulted in a much lower pregnancy rate compared to the alternative system using fresh ESCs. Furthermore, cultivated frozen ESCs showed considerably decreased release of prolactin, IGFBP-1, and IL-6 compared to fresh ESCs, indicating that cryopreservation negatively affects their decidualization potential and cytokine production., Conclusions: Altogether, this data illustrates the need for optimization and improvement of the existing autologous endometrial co-culture systems.

Published
2016
Full Text
View/download PDF

17. Secretion of immunoregulatory cytokines by mesenchymal stem cells.

Author

Kyurkchiev D, Bochev I, Ivanova-Todorova E, Mourdjeva M, Oreshkova T, Belemezova K, and Kyurkchiev S

Abstract

According to the minimal criteria of the International Society of Cellular Therapy, mesenchymal stem cells (MSCs) are a population of undifferentiated cells defined by their ability to adhere to plastic surfaces when cultured under standard conditions, express a certain panel of phenotypic markers and can differentiate into osteogenic, chondrogenic and adipogenic lineages when cultured in specific inducing media. In parallel with their major role as undifferentiated cell reserves, MSCs have immunomodulatory functions which are exerted by direct cell-to-cell contacts, secretion of cytokines and/or by a combination of both mechanisms. There are no convincing data about a principal difference in the profile of cytokines secreted by MSCs isolated from different tissue sources, although some papers report some quantitative but not qualitative differences in cytokine secretion. The present review focuses on the basic cytokines secreted by MSCs as described in the literature by which the MSCs exert immunodulatory effects. It should be pointed out that MSCs themselves are objects of cytokine regulation. Hypothetical mechanisms by which the MSCs exert their immunoregulatory effects are also discussed in this review. These mechanisms may either influence the target immune cells directly or indirectly by affecting the activities of predominantly dendritic cells. Chemokines are also discussed as participants in this process by recruiting cells of the immune systems and thus making them targets of immunosuppression. This review aims to present and discuss the published data and the personal experience of the authors regarding cytokines secreted by MSCs and their effects on the cells of the immune system.

Published
2014
Full Text
View/download PDF

18. Cells isolated from human glioblastoma multiforme express progesterone-induced blocking factor (PIBF).

Author

Kyurkchiev D, Naydenov E, Tumangelova-Yuzeir K, Ivanova-Todorova E, Belemezova K, Bochev I, Minkin K, Mourdjeva M, Velikova T, Nachev S, and Kyurkchiev S

Subjects
Cell Separation, Glioblastoma pathology, Humans, Immunohistochemistry methods, Neoplastic Stem Cells cytology, RNA, Messenger metabolism, Tumor Cells, Cultured, Glioblastoma metabolism, Neoplastic Stem Cells metabolism, Pregnancy Proteins metabolism, Progesterone metabolism, Suppressor Factors, Immunologic metabolism
Abstract

Glioblastoma multiforme (GBM) is the most common and malignant tumor in the central nervous system. One of the contemporary hypotheses postulates that its pathogenesis is associated with the cancer stem cells (CSCs) which originate from mutations in the normal neural stem cells residing in their specific "niches." Simultaneously with its aggressive development the tumor suppresses the local immune system by different secreted and/or cell expressed factors. Progesterone-induced blocking factor (PIBF) is an immunomodulatory protein with known role in the regulation of the immune response in the reproductive system. Expression of PIBF has been described in some tumors as one of the factors suppressing the anti-tumor immunity. The aim of the present study was to check for the expression of PIBF from cells isolated from six GBMs. To characterize the cultured cells and to study the PIBF expression confocal microscopy, flow cytometry, ELISA, and real-time PCR were used. The results obtained showed expression of markers typical for cancer CSCs and secretion of interleukin 6 by the GBM-derived cultured cells. The results convincingly prove that PIBF is intracellularly expressed by the cultured cells from the all six GBM samples, and this fact is confirmed by three different methods-flow cytometry, confocal microscopy, and real-time PCR. This paper reports for the first time the expression of PIBF by GBM-derived cells cultured in vitro and reveals a new aspect of the immunosuppressive mechanism used by GBM in escaping the immune control.

Published
2014
Full Text
View/download PDF

19. Conditioned medium from adipose tissue-derived mesenchymal stem cells induces CD4+FOXP3+ cells and increases IL-10 secretion.

Author

Ivanova-Todorova E, Bochev I, Dimitrov R, Belemezova K, Mourdjeva M, Kyurkchiev S, Kinov P, Altankova I, and Kyurkchiev D

Subjects
Apoptosis drug effects, Apoptosis immunology, CD4-Positive T-Lymphocytes drug effects, CD4-Positive T-Lymphocytes immunology, Cells, Cultured, Chemokines metabolism, Humans, Interleukin-2 Receptor alpha Subunit metabolism, Interleukin-6 metabolism, Lymphocyte Count, Mesenchymal Stem Cells drug effects, T-Lymphocytes, Helper-Inducer cytology, T-Lymphocytes, Helper-Inducer drug effects, T-Lymphocytes, Helper-Inducer metabolism, Up-Regulation drug effects, Adipose Tissue cytology, CD4-Positive T-Lymphocytes cytology, CD4-Positive T-Lymphocytes metabolism, Culture Media, Conditioned pharmacology, Forkhead Transcription Factors metabolism, Interleukin-10 metabolism, Mesenchymal Stem Cells cytology
Abstract

Mesenchymal stem cells (MSCs) are a new and promising tool for therapy of autoimmune disorders. In recent years their possibility to take part in the modulation of the immune response is discussed. The exact mechanisms for immunoregulation realized by MSCs are not clear yet, but interactions with other immunoregulatory cells may be involved in this process. The investigation of the influence of MSCs on the expression of FoxP3 and cytokine secretion by T helper cells was the aim of this study. T helper cells were isolated from PBMCs by magnetic separation and MSCs were isolated from human adipose tissue, and CD4⁺ T cells were cultured with conditional medium of MSCs. The methods which were used include flow cytometry, ELISA, and Human Proteome profiler kits. The results demonstrated that secretory factors in MSCs conditional medium lead to increased expression of FoxP3 and increased secretion of IL-10 by T helpers. The obtained results give us opportunity to discuss the interaction between two kinds of immunoregulatory cells: MSCs and FoxP3⁺ T helpers. We suppose that this interaction leads to increased number of immunosuppressive helpers which secrete IL-10. MSCs provide some of their immunosuppressive functions acting on T regulatory cells, and we believe that IL-6 secreted by MSCs is involved in this process.

Published
2012
Full Text
View/download PDF

20. Adipose tissue-derived mesenchymal stem cells are more potent suppressors of dendritic cells differentiation compared to bone marrow-derived mesenchymal stem cells.

Author

Ivanova-Todorova E, Bochev I, Mourdjeva M, Dimitrov R, Bukarev D, Kyurkchiev S, Tivchev P, Altunkova I, and Kyurkchiev DS

Subjects
Adult, B7-1 Antigen metabolism, B7-2 Antigen metabolism, Bone Marrow Cells metabolism, Cells, Cultured, Chemokines metabolism, Coculture Techniques, Cytokines metabolism, Dendritic Cells metabolism, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Granulocyte-Macrophage Colony-Stimulating Factor pharmacology, Humans, Interleukin-10 metabolism, Interleukin-4 pharmacology, Leukocytes, Mononuclear cytology, Leukocytes, Mononuclear drug effects, Lipopolysaccharide Receptors metabolism, Mesenchymal Stem Cells drug effects, Middle Aged, Adipose Tissue cytology, Bone Marrow Cells cytology, Cell Differentiation, Dendritic Cells cytology, Mesenchymal Stem Cells cytology
Abstract

Both mesenchymal stem cells (MSCs) and dendritic cells (DCs) are engaged in the regulation of the immune response parallel to their numerous functions. The main objective of this study was to compare the effects of mesenchymal stem cells isolated from human adipose tissue or human bone marrow on the expression of specific cell surface markers as well as the secretion of some cytokines by monocyte-derived dendritic cells. The set of methods used includes cell cultures, magnetic beads isolation of cells, flow cytometry, ELISA and proteome profiler kit assays. The results obtained show that MSCs isolated from human adipose tissue are more potent immunomodulators of differentiation of human DCs in comparison to the bone marrow-derived MSCs. In both cases the percentages of CD14+ cells were increased in co-cultures of MSCs and DCs and at the same time down-regulated the expression of CD80, CD86 and CD83 as in all experiments the effect of adipose tissue MSCs was stronger. Similarly, the secretion of IL-10 by dendritic cells was up-regulated in co-cultures of MSCs and dendritic cells and the effect was stronger when adipose tissue-derived MSCs were used. Taken together all results presented reveal the higher potential of the adipose tissue-derived MSCs to inhibit the differentiation and expression of functionally important co-stimulatory molecules on the surface of monocyte-derived dendritic cells than the bone marrow-derived MSCs.

Published
2009
Full Text
View/download PDF

21. HLA-G expression is up-regulated by progesterone in mesenchymal stem cells.

Author

Ivanova-Todorova E, Mourdjeva M, Kyurkchiev D, Bochev I, Stoyanova E, Dimitrov R, Timeva T, Yunakova M, Bukarev D, Shterev A, Tivchev P, and Kyurkchiev S

Subjects
Adipose Tissue immunology, Adipose Tissue metabolism, Adult, Aged, Aged, 80 and over, Cells, Cultured, Decidua immunology, Female, HLA-G Antigens, Humans, Mesenchymal Stem Cells drug effects, Middle Aged, Pregnancy, Progesterone pharmacology, Progestins pharmacology, Up-Regulation, HLA Antigens biosynthesis, Histocompatibility Antigens Class I biosynthesis, Mesenchymal Stem Cells immunology, Progesterone metabolism
Abstract

Problem: Maternal immune response to fetal tissues is modified in such way that it favors the development of pregnancy. Human leukocyte antigen (HLA)-G, progesterone and mesenchymal stem cells (MSCs) have been identified as potent immunomodulatory agents in different experimental systems and the interactions between these three factors are studies in this paper., Method of Study: Human MSCs are isolated from human adipose tissue, bone marrow and decidua are cultured in the presence of progesterone and the expression of HLA-G is followed-up at protein and mRNA levels., Results: The MSCs cultured in the presence of progesterone express increased levels of both cell surface and cytoplasmic HLA-G when compared with the control MSCs., Conclusion: Progesterone up-regulates the expression by MSCs of HLA-G which is a major player in maintenance of the immune balance between the mother and the fetus. MSCs are newly detected targets of progesterone with well documented immunomodulatory activity.

Published
2009
Full Text
View/download PDF

22. Mesenchymal stem cells from human bone marrow or adipose tissue differently modulate mitogen-stimulated B-cell immunoglobulin production in vitro.

Author

Bochev I, Elmadjian G, Kyurkchiev D, Tzvetanov L, Altankova I, Tivchev P, and Kyurkchiev S

Subjects
Adipogenesis drug effects, Adipose Tissue drug effects, Adult, Aged, Aged, 80 and over, Antibody Formation drug effects, Antigens, CD metabolism, B-Lymphocytes cytology, Bone Marrow Cells drug effects, Cell Proliferation drug effects, Cell Separation, Cell Shape drug effects, Cells, Cultured, Colony-Forming Units Assay, Endoglin, Fibroblasts cytology, Fibroblasts drug effects, Humans, Immunophenotyping, Mesenchymal Stem Cells drug effects, Middle Aged, Osteogenesis drug effects, Receptors, Cell Surface metabolism, Vimentin metabolism, Adipose Tissue cytology, Antibody Formation immunology, B-Lymphocytes drug effects, B-Lymphocytes immunology, Bone Marrow Cells cytology, Mesenchymal Stem Cells cytology, Mitogens pharmacology
Abstract

Mesenchymal stem cells (MSC) have been characterized as multipotent cells which are able to differentiate into several mesodermal and nonmesodermal lineage cells and this feature along with their extensive growth and comprehensive immunomodulatory properties establish them as a promising tool for therapeutic applications, including cell-based tissue engineering and treatment of immune-mediated disorders. Although bone marrow (BM) is the most common MSC source, cells with similar characteristics have been shown to be present in several other adult tissues. Adipose tissue (AT), large quantities of which can be easily obtained, represents an attractive alternative to BM in isolating adipose tissue-derived MSC (AT-MSC). BM-MSCs and AT-MSCs share some immunomodulatory properties as they are both not inherently immunogenic and suppress the proliferation of alloantigen- or mitogen-stimulated T-cells. Our purpose was to comparatively examine under appropriate in vitro conditions, phenotypes, morphology and some functional properties of BM-MSCs and AT-MSCs, such as differentiation potential and especially the ability to suppress the immunoglobulin production by mitogen-stimulated B-cells. While the morphological, immunophenotypical, colony-forming and adipogenic characteristics of both types of cells were almost identical, AT-MSCs showed less potential for osteogenic differentiation than BM-MSCs. We found that AT-MSCs not only inhibited the Ig-production but also suppressed this B-cell function to a much greater extent compared to BM-MSC. This finding supports the potential role of AT-MSCs as an alternative to BM-MSCs for clinical purposes.

Published
2008
Full Text
View/download PDF

23. Isolation of avian influenza virus in Bulgaria.

Author

Zarkov IS, Bochev I, Manvell R, and Shell W

Subjects
Animal Migration, Animals, Animals, Wild virology, Birds, Bulgaria epidemiology, Disease Reservoirs veterinary, Disease Reservoirs virology, Influenza in Birds diagnosis, Influenza in Birds transmission, Polymerase Chain Reaction methods, Polymerase Chain Reaction veterinary, Species Specificity, Influenza A virus isolation & purification, Influenza in Birds epidemiology
Published
2006
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results