1. Investigation of streptavidin-ligand biochemical interactions by IGZO thin film transistors integrated with microfluidic channels
- Author
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Jian-Jang Huang, Yu-Hwa Lo, Nian-Ting Wu, and Bo-Shuen Jiang
- Subjects
Streptavidin ,Materials science ,010401 analytical chemistry ,Metals and Alloys ,Nanotechnology ,02 engineering and technology ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,Ligand (biochemistry) ,01 natural sciences ,Signal ,Small molecule ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,chemistry.chemical_compound ,Microfluidic chamber ,chemistry ,Thin-film transistor ,Microfluidic channel ,Materials Chemistry ,Electrical and Electronic Engineering ,0210 nano-technology ,Instrumentation ,Biosensor - Abstract
In this work, we demonstrate an IGZO TFT-based biosensor integrated with a microfluidic channel that can be used to monitor biochemical reactions between proteins and small molecules. The target biomaterials are applied to a Y-shaped microfluidic chamber while the reactant signal is readout by the TFT. Using Biotin-Streptavidin reaction as an example, the specific diffusion time of biotin, streptavidin, or streptavidin-biotin complex across the fluidic channel are benchmarked. By intentionally delaying the application of one specie to another, one can study properties of biochemical reactions, such as excess and exhaust conditions of the reactants, and the formation of the products while diffusing in the channel. The proposed TFT sensor provides an excellent platform in understanding the biochemical functions for the advance of biological science.
- Published
- 2018
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