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Due to their commendable biocompatibility, regenerated silk fibroin (RSF) films have attracted considerable research interest. However, the poor mechanical properties of RSF films have limited their use in various biomedical applications. In this study, a novel, highly crystalline silk fibril was successfully extracted from silk by combining degumming with ultrasonication. Ultrasonication accelerated the development of silk nanofibrils measuring 130–200 nm on the surface of the over-degummed silk fibers, which was confirmed via scanning electron microscopy. Additionally, the crystallinity index of silk fibril was found to be significantly higher (~68%) than that of conventionally degummed silk (~54%), as confirmed by the Fourier-transform infrared (FTIR) spectroscopy results. Furthermore, the breaking strength and elongation of the RSF film were increased 1.6 fold and 3.4 fold, respectively, following the addition of 15% silk nanofibrils. Thus, the mechanical properties of the RSF film were remarkably improved by the addition of the silk nanofibrils, implying that it can be used as an excellent reinforcing material for RSF films.

Two typographical corrections are made for our recently published paper [Opt. Mater. Express 12(7), 2529 (2022)10.1364/OME.462592]. The results, discussion, and conclusion of the paper are unaffected.

Rheumatoid arthritis (RA) is a multifactorial immune-mediated disease, the pathogenesis of which involves different cell types. T-cell activation plays an important role in RA. Therefore, inhibiting T-cell activation is one of the current therapeutic strategies. Cytotoxic T-lymphocyte antigen 4-immunoglobulin (CTLA4-Ig), also known as abatacept, reduces cytokine secretion by inhibiting T-cell activation. To achieve a homeostatic therapeutic effect, CTLA4-Ig has to be administered repeatedly over several weeks, which limits its applicability in RA treatment. To overcome this limitation, we increased the number of sialic acid-capped antennas by genetically engineering the CTLA4 region to increase the therapeutic effect of CTLA4-Ig. N-acetylglucosaminyltransferase (GnT) and α2,6-sialyltransferase (α2,6-ST) were co-overexpressed in Chinese hamster ovary (CHO) cells to generate a highly sialylated CTLA4-Ig fusion protein, named ST6. The therapeutic and immunogenic effects of ST6 and CTLA4-Ig were compared. ST6 dose-dependently decreased paw edema in a mouse model of collagen-induced arthritis and reduced cytokine levels in a co-culture cell assay in a similar manner to CTLA4-Ig. ST6- and CTLA4-Ig-induced T cell-derived cytokines were examined in CD4 T cells isolated from peripheral blood mononuclear cells after cell killing through irradiation followed by flow- and magnetic-bead-assisted separation. Interestingly, compared to CTLA4-Ig, ST6 was substantially less immunogenic and more stable and durable. Our data suggest that ST6 can serve as a novel, less immunogenic therapeutic strategy for patients with RA.

Recently, the electrospun silk web has been intensively studied in terms of its biomedical applications, including tissue engineering scaffolds, due to its good biocompatibility, cytocompatibility, and biodegradability. In this study, the effect of relative humidity (RH) conditions on the morphology of electrospun silk fiber and the electrospinning production rate of silk solution was examined. In addition, the effect of RH on the molecular conformation of electrospun silk web was examined using Fourier transform infrared (FTIR) spectroscopy. As RH was increased, the maximum electrospinning rate of silk solution and fiber diameter of the resultant electrospun silk web were decreased. When RH was increased to 60%, some beads were observed, which showed that the electrospinnability of silk formic acid solution deteriorated with an increase in RH. The FTIR results showed that electrospun silk web was partially β-sheet crystallized and RH did not affect the molecular conformation of silk.

There is an urgent need to find antidepressants that can be administered for long periods without inducing severe side effects to replace conventional antidepressants that control monoamine levels, such as tricyclic antidepressants (TCAs), monoamine oxidase inhibitors (MAOIs), and selective serotonin reuptake inhibitors (SSRI). We sought to determine the antidepressant effects of Fraxinus rhynchophylla Hance (F. rhynchophylla Hance, FX) and its components on a reserpine-induced mouse model. One hour after oral administration of FX (30, 50, and 100 mg/kg), esculin (50 mg/kg), esculetin (50 mg/kg), fraxin (50 mg/kg), and fluoxetine (20 mg/kg), reserpine was delivered intraperitoneally to mice. Behavioral experiments were conducted to measure anxiety and depressive-like behaviors after 10 days of administration. FX and its components increased the number of entries into the center of an open field as well as distance traveled within it and decreased immobility duration in the forced swim and tail suspension tests. Reserpine-induced increases in plasma corticosterone concentrations were attenuated by the administration of FX and its components, which were also found to decrease the reserpine-induced enhancement of mRNA levels of interleukin (IL)-12 p40, IL-6, and tumor necrosis factor (TNF)-α, pro-inflammatory cytokines. Finally, the diminished expressions of hippocampal phosphorylated cAMP response element-binding protein (pCREB) and brain-derived neurotrophic factor (BDNF) by reserpine were increased by FX and its components. Our results suggest that FX and its components regulate anxiety and depressive-like behaviors through stress hormones, immune regulation, and the activation of neuroprotective mechanisms, further supporting the potential of FX and its components as antidepressants.

There is an urgent need to find antidepressants that can be administered for long periods without inducing severe side effects to replace conventional antidepressants that control monoamine levels, such as tricyclic antidepressants (TCAs), monoamine oxidase inhibitors (MAOIs), and selective serotonin reuptake inhibitors (SSRI). We sought to determine the antidepressant effects of Fraxinus rhynchophylla Hance (F. rhynchophylla Hance, FX) and its components on a reserpine-induced mouse model. One hour after oral administration of FX (30, 50, and 100 mg/kg), esculin (50 mg/kg), esculetin (50 mg/kg), fraxin (50 mg/kg), and fluoxetine (20 mg/kg), reserpine was delivered intraperitoneally to mice. Behavioral experiments were conducted to measure anxiety and depressive-like behaviors after 10 days of administration. FX and its components increased the number of entries into the center of an open field as well as distance traveled within it and decreased immobility duration in the forced swim and tail suspension tests. Reserpine-induced increases in plasma corticosterone concentrations were attenuated by the administration of FX and its components, which were also found to decrease the reserpine-induced enhancement of mRNA levels of interleukin (IL)-12 p40, IL-6, and tumor necrosis factor (TNF)-α, pro-inflammatory cytokines. Finally, the diminished expressions of hippocampal phosphorylated cAMP response element-binding protein (pCREB) and brain-derived neurotrophic factor (BDNF) by reserpine were increased by FX and its components. Our results suggest that FX and its components regulate anxiety and depressive-like behaviors through stress hormones, immune regulation, and the activation of neuroprotective mechanisms, further supporting the potential of FX and its components as antidepressants.

Geekbench is one of the most referenced cross-platform benchmarks in the mobile world. Most of its workloads are synthetic but some of them aim to simulate real-world behavior. In the mobile world, its microarchitectural behavior has been reported rarely since the hardware profiling features are limited to the public. As a popular mobile performance workload, it is hard to find Geekbench&rsquo, s microarchitecture characteristics in mobile devices. In this paper, a thorough experimental study of Geekbench performance characterization is reported with detailed performance metrics. This study also identifies mobile system on chip (SoC) microarchitecture impacts, such as the cache subsystem, instruction-level parallelism, and branch performance. After the study, we could understand the bottleneck of workloads, especially in the cache sub-system. This means that the change of data set size directly impacts performance score significantly in some systems and will ruin the fairness of the CPU benchmark. In the experiment, Samsung&rsquo, s Exynos9820-based platform was used as the tested device with Android Native Development Kit (NDK) built binaries. The Exynos9820 is a superscalar processor capable of dual issuing some instructions. To help performance analysis, we enable the capability to collect performance events with performance monitoring unit (PMU) registers. The PMU is a set of hardware performance counters which are built into microprocessors to store the counts of hardware-related activities. Throughout the experiment, functional and microarchitectural performance profiles were fully studied. This paper describes the details of the mobile performance studies above. In our experiment, the ARM DS5 tool was used for collecting runtime PMU profiles including OS-level performance data. After the comparative study is completed, users will understand more about the mobile architecture behavior, and this will help to evaluate which benchmark is preferable for fair performance comparison.

Scales of Cyphochilus white beetles present one of the strongest optical scattering materials in nature. However, the intricate optical fibrillar network nanostructure inside the scales has been difficult to mimic. Here, characteristic structural parameters inside Cyphochilus scales – mean fiber diameter, diameter distribution, filling fraction, and structural anisotropy – are replicated in synthetic nanofibrous materials to functionally mimic the biological material. To fabricate the synthetic nanostructure, electrospinning is chosen because this conventional technique is amenable to nanomanufacturing. The optimized parameters in electrospun structures are found to be only slightly different from those in Cyphochilus scales. At the optimum, electrospun structures exhibit even stronger optical scattering than Cyphochilus scales. An electrospun film with the similar characteristic structural parameters as those in Cyphochilus scales gives two resonance peaks in visible reflectance spectrum in the limit of a uniform fiber diameter, giving a purple structural color. As the distribution of diameter increases appreciably to experimentally achievable degrees, the resonance peaks broaden and the reflectance spectrum becomes relatively flat, resulting in disappearance of the structural color. These results support that controllable fibrous nanostructures that exceed the exceptionally strong broadband optical scattering found among living organisms can be volume-produced.

Bangpungtongsung-san (BTS) is a traditional Korean medicine consisting of 18 herbs, some which have antidepressant effects. Here, we used an animal model of reserpine-induced depression and lipopolysaccharide (LPS)-stimulated BV2 microglia to assess the antidepressant and anti-neuroinflammatory effects of BTS. Aside from a control group, C57BL/6 mice were administered reserpine (0.5 mg/kg) daily for 10 days via intraperitoneal injection. BTS (100, 300, or 500 mg/kg), vehicle (PBS), or fluoxetine (FXT, 20 mg/kg) was administered orally 1 h before reserpine treatment. Following treatment, a forced swimming test (FST), tail suspension test (TST), and open field test (OFT) were performed, and immobility time and total travel distance were measured. Administration of BTS not only reduced immobility time in the FST and TST but also significantly increased the total travel distance in the OFT. Furthermore, reserpine-treated mice showed significantly elevated serum levels of corticosterone, a stress hormone; however, treatment with BTS significantly reduced corticosterone levels, similar to FXT treatment. Serotonin in reserpine-treated mice was significantly reduced compared to that in control mice, while BTS mice exhibited increased serotonin levels. BTS mice showed increased expression of brain-derived neurotrophic factor (BDNF) and a higher ratio of phosphorylated cAMP response element-binding protein (p-CREB) to CREB (p-CREB/CREB) in the hippocampus. Additionally, reserpine-treated mice exhibited significantly elevated mRNA levels of pro-inflammatory cytokines, but BTS mice showed reduced mRNA levels of interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α in the hippocampus. To further demonstrate the anti-neuroinflammatory effects of BTS in vitro, we examined its anti-neuroinflammatory and neuroprotective effects in lipopolysaccharide (LPS)-stimulated BV2 microglia. BTS significantly reduced the levels of NO, inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, TNF-α, IL-1β, and IL-6 in a dose-dependent manner via a decrease in the expression of nuclear factor (NF)-κB p65. Furthermore, the neuroprotective factor heme oxygenase-1 (HO-1) was upregulated via the nuclear factor-E2-related factor 2 (NRF2)/CREB pathway. Taken together, our data suggest that BTS has considerable potential as an anti-neuroinflammation and antidepressant agent, as it has clear effects on depressive behaviors and associated factors caused by reserpine-induced depression.

Objective To determine patients' perceptions of the benefits of participating in outpatient cardiac rehabilitation and the reasons why some decline to take part. Method Data collected included patients' responses to the self-administrated ‘Outpatient Cardiac Rehabilitation Program Evaluation' form, after attending a cardiac rehabilitation program. The evaluation involved analysis of 9 binary and open ended questions. A retrospective study was completed on data collected from January 2010 to December 2015 (6 years) and included 643 adult cases comprising 500 men and 143 women. A between subject t-Test was used to compare patient means before and after attendance of perceived changes to their lifestyle, and overall sense of physical and emotional well-being. Fishers Exact Test was used to compare attendance percentages, gender distribution, and primary diagnosis. Results Two hundred and seventy nine (43.4%) of the 643 invited patients participated in the cardiac rehabilitation program, while 364 (56.6%) declined, with this result being significantly lower (p Conclusion Considering the reported benefits of attending cardiac rehabilitation, the number of people who decline to attend has important implications for their health and related health system costs related to ongoing disease.

Treatment with the antihypertensive agent reserpine depletes monoamine levels, resulting in depression. In the present study, we evaluated the antidepressant effects of Gyejibokryeong-hwan (GBH), a traditional Korean medicine, in a mouse model of reserpine-induced depression. Mice were treated with reserpine (0.5 mg·kg−1, i.p.) or phosphate-buffered saline (PBS, i.p., normal) once daily for 10 days. GBH (50, 100, 300, and 500 mg·kg−1), PBS (normal, control), fluoxetine (FXT, 20 mg·kg−1), or amitriptyline (AMT, 30 mg·kg−1) was administered orally 1 h prior to reserpine treatment. Mouse behavior was examined in the forced swim test (FST), tail suspension test (TST), and open-field test (OFT) following completion of the treatment protocol. Administration of GBH reduced immobility time in the FST and TST and significantly increased the total distance traveled in the OFT. Plasma serotonin levels were significantly lower in control mice than in normal mice, although these decreases were significantly attenuated to a similar extent by treatment with GBH, FXT, or AMT. Reserpine-induced increases in plasma corticosterone were also attenuated by GBH treatment. Moreover, GBH attenuated reserpine-induced increases in interleukin- (IL-) 1β, IL-6, and tumor necrosis factor- (TNF-) α mRNA expression in the hippocampus. In addition, GBH mice exhibited increased levels of brain-derived neurotrophic factor (BDNF) and a higher ratio of phosphorylated cAMP response element-binding protein (p-CREB) to CREB (p-CREB/CREB) in the hippocampus. Our results indicated that GBH can ameliorate depressive-like behaviors, affect the concentration of mood-related hormones, and help to regulate immune/endocrine dysfunction in mice with reserpine-induced depression, likely via activation of the BDNF-CREB pathway. Taken together, these findings indicate that GBH may be effective in treating patients with depression.

Benzalkonium chloride, diazolidinyl urea, and imidazolidinyl urea are commonly used preservatives in cosmetics. Recent reports suggested that these compounds may have cellular and systemic toxicity in high concentration. In addition, diazolidinyl urea and imidazolidinyl urea are known formaldehyde (FA) releasers, raising concerns for these cosmetic preservatives. In this study, we investigated the effects of benzalkonium chloride, diazolidinyl urea, and imidazolidinyl urea on ROS-dependent apoptosis of rat neural progenitor cells (NPCs) in vitro. Cells were isolated and cultured from embryonic day 14 rat cortices. Cultured cells were treated with 1-1,000 nM benzalkonium chloride, and 1-50 μM diazolidinyl urea or imidazolidinyl urea at various time points to measure the reactive oxygen species (ROS). PI staining, MTT assay, and live-cell imaging were used for cell viability measurements. Western blot was carried out for cleaved caspase-3 and cleaved caspase-8 as apoptotic protein markers. In rat NPCs, ROS production and cleaved caspase-8 expression were increased while the cell viability was decreased in high concentrations of these substances. These results suggest that several cosmetic preservatives at high concentrations can induce neural toxicity in rat brains through ROS induction and apoptosis.

Owing to the excellent biocompatibility of silk fibroins (SFs) and ease of fabrication of nano-fibrous webs by the electro-spinning technique, electro-spun SF webs have attracted the attention of researchers for various biomedical applications, including their use as tissue engineering scaffolds and membranes for guided bone regeneration. In this work, the effect of the molecular weight (MW) and concentration of SFs on the structure and properties of the electro-spun SF webs was examined. The fiber morphology and porosity of these SF webs were strongly affected by the viscosity of the SF dope solution. It was found that the electro-spinning rate of the SF solution could be increased significantly (7.5 fold) by controlling the MW and concentration of the SF. Interestingly, as the SF MW and concentration (i.e., s​olution viscosity) increased, the extent of β-sheet crystallization of the SF decreased, leading to a decrease in the overall crystallinity. The strength and elongation of the electro-spun SF web decreased with an increase in the web porosity (i.e., increasing SF concentration) and a decrease in the MW of the SF.

Purpose The purpose of this study was to determine the frequency of aberrant right subclavian artery (ARSA) among unselected fetuses and to evaluate its association with chromosomal abnormalities and other congenital anomalies. Methods In all, 7,547 fetuses (gestational age, 20 to 34 weeks) were examined using routine antenatal sonography at our institution between April 2014 and September 2015. The right subclavian artery was assessed using grayscale and color Doppler ultrasonography in the transverse 3-vessel and tracheal view, and confirmed in the coronal plane. Results ARSA was found in 28 fetuses (0.4%). Further, 27 of these 28 fetuses were euploid (96.4%). Trisomy 18 was the only chromosomal anomaly (3.6%) found in the study sample. ARSA was an isolated finding in 23 of the 28 cases (82.1%). In the remaining three cases (10.7%), ARSA was accompanied with extracardiac anomalies. Other cardiac defects were present in three cases (10.7%). Conclusion Isolated ARSA does not seem to be associated with a significantly increased risk of aneuploidy. However, the possibility of fetal karyotyping, which is a more invasive procedure, should be discussed in the light of the overall risk of the fetus.

Owing to the excellent cyto-compatibility of silk fibroin (SF) and the simple fabrication of nano-fibrous webs, electro-spun SF webs have attracted much research attention in numerous biomedical fields. Because the production rate of electro-spun webs is strongly dependent on the electro-spinning rate used, the electro-spinning rate becomes more important. In the present study, to improve the electro-spinning rate of SF solutions, various electric fields were applied during electro-spinning of SF, and its effects on the maximum electro-spinning rate of SF solution as well as diameters and molecular conformations of the electro-spun SF fibers were examined. As the electric field was increased, the maximum electro-spinning rate of the SF solution also increased. The maximum electro-spinning rate of a 13% SF solution could be increased 12×by increasing the electric field from 0.5kV/cm (0.25mL/h) to 2.5kV/cm (3.0mL/h). The dependence of the fiber diameter on the present electric field was not significant when using less-concentrated SF solutions (7-9% SF). On the other hand, at higher SF concentrations the electric field had a greater effect on the resulting fiber diameter. The electric field had a minimal effect of the molecular conformation and crystallinity index of the electro-spun SF webs.

In the Fourth Industrial Revolution environment, plenty of automatic and smart software in diverse fields will come out, such as virtual reality/augmented reality (VR/AR), autonomous robots and vehicles, smart factoring, and so on. In Korea, especially, one important issue is the cyber&ndash, physical system (CPS), used for monitoring and controlling the smart and automatic system in a smart city. This kind of system, therefore, needs to have good performance, otherwise, it may not respond in time. To solve this, we propose a code visualization approach to reduce code complexity based on a static analysis, which identifies bad codes against performance. To ensure better performance, we make our queries identify performance degradation factors, store statically analyzed data into database (DB) tables, and visualize bad operation patterns. For performance improvement, we can refactor with them. As a result, we reduce the code complexity of CPS-based software to obtain good performance. With this approach, we expect to have better performance and a reduction in the complexity of CPS software without even power consumption.

Microglia, the central nervous system’s innate immune cells, mediate neuroinflammation and are implicated in a variety of neuropathologies. The present study investigated the antineuroinflammatory and neuroprotective effects of Gyejibokryeong-hwan (GBH), a traditional Korean medicine, in lipopolysaccharide- (LPS-) stimulated murine BV2 microglia. BV2 cells were pretreated with GBH, fluoxetine (FXT), or amitriptyline (AMT) for 1 h and then stimulated with LPS (100 ng/mL). The expression levels of nitric oxide (NO), cytokines, and chemokines were determined by the Griess method, ELISA, or real-time PCR. Western blotting was used to measure various transcription factors and mitogen activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K)/Akt activity. GBH significantly reduced the levels of NO, inducible nitric oxide synthase (iNOS), cyclooxygenase- (COX-) 2, tumor necrosis factor- (TNF-) α, interleukin- (IL-) 1β, IL-6, macrophage inhibitory protein- (MIP-) 1α, macrophage chemoattractant protein- (MCP-) 1, and IFN-γ inducible protein- (IP-) 10, regulated upon activation normal T cell expressed sequence (RANTES) in a dose-dependent manner. Expression of nuclear factor- (NF-) κB p65 was significantly decreased and phosphorylation of extracellular signal-regulated kinase (Erk), c-Jun NH2-terminal kinase (JNK), and PI3K/Akt by GBH, but not p38 MAPK, was decreased. Furthermore, production of anti-inflammatory cytokine IL-10 was increased and Heme oxygenase-1 (HO-1) was upregulated via the nuclear factor-E2-related factor 2 (NRF2)/cAMP response element-binding protein (CREB) pathway, collectively indicating the neuroprotective effects of GBH. We concluded that GBH may suppress neuroinflammatory responses by inhibiting NF-κB activation and upregulating the neuroprotective factor, HO-1. These results suggest that GBH has potential as anti-inflammatory and neuroprotective agents against microglia-mediated neuroinflammatory disorders.

Objective: This study aimed to examine the effects of attachment and shared time between mother and child and self-esteem on subjective well-being in late childhood. Methods: A total of 329 fifth and sixth graders in elementary school (183 boys and 146 girls) participated in the study. They responded to questionnaires regarding attachment and shared time between mother and child and the self-esteem and subjective well-being of children. The data were analyzed using SEM. Results: Attachment and shared time between mother and child had an indirect effect through self-esteem as well as a direct effect on the subjective well-being of children. Children who formed secure attachments and spent more time with their mothers showed a higher level of subjective well-being. In addition, when children kept a good relationship with their mother quantitatively as well as qualitatively, they acknowledged their own capability and value positively, which led to higher subjective well-being. Conclusion: Findings emphasize that mother-child relationships play significant roles in predicting both the subjective well-being and self-esteem of children. The importance of self-esteem was identified in late childhood based on its relative influence on subjective well-being compared to mother-child relationships.

Triclosan is an antimicrobial or sanitizing agent used in personal care and household products such as toothpaste, soaps, mouthwashes and kitchen utensils. There are increasing evidence of the potentially harmful effects of triclosan in many systemic and cellular processes of the body. In this study, we investigated the effects of triclosan in the survivability of cultured rat neural stem cells (NSCs). Cortical cells from embryonic day 14 rat embryos were isolated and cultured in vitro. After stabilizing the culture, triclosan was introduced to the cells with concentrations ranging from 1 μM to 50 μM and in varied time periods. Thereafter, cell viability parameters were measured using MTT assay and PI staining. TCS decreased the cell viability of treated NSC in a concentration-dependent manner along with increased expressions of apoptotic markers, cleaved caspase-3 and Bax, while reduced expression of Bcl2. To explore the mechanisms underlying the effects of TCS in NSC, we measured the activation of MAPKs and intracellular ROS. TCS at 50 μM induced the activations of both p38 and JNK, which may adversely affect cell survival. In contrast, the activities of ERK, Akt and PI3K, which are positively correlated with cell survival, were inhibited. Moreover, TCS at this concentration augmented the ROS generation in treated NSC and depleted the glutathione activity. Taken together, these results suggest that TCS can induce neurodegenerative effects in developing rat brains through mechanisms involving ROS activation and apoptosis initiation.

Hibiscus syriacus L. (Malvaceae) is an important ornamental shrub in horticulture and has been widely used as a medical material in Asia. The aim of this study was to assess the antidepressant and neuroprotective effects of a root bark extract of H. syriacus (HSR) and to investigate the underlying molecular mechanisms. Using an animal model of restraint stress, we investigated the effects of HSR on depressive-like behaviors and on the expression levels of serotonin, corticosterone, and neurotrophic factors in the brain. The mice were exposed to restraint stress for 2 h per day over a period of 3 weeks and orally treated with HSR (100, 200, or 400 mg/kg/day). We also examined the neuroprotective effect of HSR using corticosterone-treated human neuroblastoma SK-N-SH cells. The cells were incubated with the extract for 24 h, followed by corticosterone stimulation for 1 h, and then cell viability assay, cellular ATP assay, mitochondrial membrane potential (MMP) assay, cellular reactive oxygen species (ROS) assay, and western blotting were used to investigate the neuroprotective effects of HSR. Administration of HSR not only reduced the immobility times of the restraint-stressed mice in the forced swimming and tail suspension tests, but also significantly increased sucrose preference in the sucrose preference test. In addition, HSR significantly reduced the plasma levels of corticosterone and increased the brain levels of serotonin. The extract also increased the phosphorylation level of cyclic AMP response element-binding (CREB) protein and the expression level of brain-derived neurotrophic factor (BDNF). The in vitro assays showed that HSR pretreatment increased cell viability and ATP levels, recovered MMP, decreased ROS levels, and increased the expression of CREB and BDNF in corticosterone-induced neurotoxicity. Taken together, our data suggest that HSR may have the potential to control neuronal cell damage and depressive behaviors caused by chronic stress.

Prolonged exposure to stress can affect mood and cognition and lead to mood disorders. Research on stress-associated mood disorders is important in modern society as people are increasingly exposed to unavoidable stressors. We used a mouse model with 2 weeks of exposure to electric foot shock and restraint, to determine the effect ofFraxinus rhynchophyllaHance (FX) extract on chronic stress-induced depression. We measured the effect of FX extract using various physiological, behavioral, and biochemical measures. FX extract ameliorated chronic stress-induced body and relative liver weight loss and improved depressive-like behaviors in the open field and forced swim tests. In addition, plasma cortisol and serotonin levels in stress-induced mice following FX treatment were similar to normal mice, and the elevation of proinflammatory cytokines was prevented. Moreover, FX treatment increased the expression of phosphorylated cyclic adenosine-3′,5′-monophosphate response element-binding protein (pCREB)/brain-derived neurotrophic factor (BDNF). Further experiments confirmed the efficacy of FX extract by showing similar results using esculin and esculetin, compounds extracted from FX. Taken together, these results indicate that FX extract has an antidepressant effect on chronic stress-induced depression by associating signaling with neuroinflammation and neurogenesis.

Hibiscus syriacus L. (Malvaceae) is an important ornamental shrub in horticulture and has been widely used as a medical material in Asia. The aim of this study was to assess the antidepressant and neuroprotective effects of a root bark extract of H. syriacus (HSR) and to investigate the underlying molecular mechanisms. Using an animal model of restraint stress, we investigated the effects of HSR on depressive-like behaviors and on the expression levels of serotonin, corticosterone, and neurotrophic factors in the brain. The mice were exposed to restraint stress for 2 h per day over a period of 3 weeks and orally treated with HSR (100, 200, or 400 mg/kg/day). We also examined the neuroprotective effect of HSR using corticosterone-treated human neuroblastoma SK-N-SH cells. The cells were incubated with the extract for 24 h, followed by corticosterone stimulation for 1 h, and then cell viability assay, cellular ATP assay, mitochondrial membrane potential (MMP) assay, cellular reactive oxygen species (ROS) assay, and western blotting were used to investigate the neuroprotective effects of HSR. Administration of HSR not only reduced the immobility times of the restraint-stressed mice in the forced swimming and tail suspension tests, but also significantly increased sucrose preference in the sucrose preference test. In addition, HSR significantly reduced the plasma levels of corticosterone and increased the brain levels of serotonin. The extract also increased the phosphorylation level of cyclic AMP response element-binding (CREB) protein and the expression level of brain-derived neurotrophic factor (BDNF). The in vitro assays showed that HSR pretreatment increased cell viability and ATP levels, recovered MMP, decreased ROS levels, and increased the expression of CREB and BDNF in corticosterone-induced neurotoxicity. Taken together, our data suggest that HSR may have the potential to control neuronal cell damage and depressive behaviors caused by chronic stress.

Prolonged exposure to stress can affect mood and cognition and lead to mood disorders. Research on stress-associated mood disorders is important in modern society as people are increasingly exposed to unavoidable stressors. We used a mouse model with 2 weeks of exposure to electric foot shock and restraint, to determine the effect of Fraxinus rhynchophylla Hance (FX) extract on chronic stress-induced depression. We measured the effect of FX extract using various physiological, behavioral, and biochemical measures. FX extract ameliorated chronic stress-induced body and relative liver weight loss and improved depressive-like behaviors in the open field and forced swim tests. In addition, plasma cortisol and serotonin levels in stress-induced mice following FX treatment were similar to normal mice, and the elevation of proinflammatory cytokines was prevented. Moreover, FX treatment increased the expression of phosphorylated cyclic adenosine-3′,5′-monophosphate response element-binding protein (pCREB)/brain-derived neurotrophic factor (BDNF). Further experiments confirmed the efficacy of FX extract by showing similar results using esculin and esculetin, compounds extracted from FX. Taken together, these results indicate that FX extract has an antidepressant effect on chronic stress-induced depression by associating signaling with neuroinflammation and neurogenesis.

BACKGROUND/OBJECTIVES: This study measured the effects of a taste education program developed in Korea on food neophobia and willingness to try novel foods in school children. SUBJECTS/METHODS: One-hundred and twenty school children (aged 7-9 years) residing in Seoul participated in 12 sessions of a taste education program for 3 months. The Korean taste education program was adapted from “Les classes du gout” by J. Puisais and modified to suit a Korean education environment. The study subjected school children to pre- and post-programs on food neophobia and willingness to try novel foods (WTNF), in addition to children’s food neophobia in their parents. A total of 101 survey data were analyzed using SPSS 18.0. RESULTS: Regarding the effects of taste education, scores of food neophobia significantly decreased (P ＜ 0.01) in the posttest, mean (m) score (4.10 ± 1.19) decreased compared to the pretest (4.39 ± 1.00), and WTNF significantly increased (P ＜ 0.001) in the pretest (m) score (0.48 ± 0.33) compared to the pretest (0.32 ± 0.34). This result indicates verification of the study hypothesis. CONCLUSIONS: Food neophobia scale (FNS), an index that measures personal food preference [1,2], showed a very weak correlation with behavioral willingness to taste novel foods (WTNF). Therefore, it is expected that the two scales measure different things. However, considering that the traits of food neophobia are not easily changed, the taste education program was administered in a remarkably effective manner.

This study examined short-term longitudinal reciprocal relationships between maternal parenting behavior and preschoolers' compliance/noncompliance, as well as stability in both maternal parenting behavior and preschoolers' compliance/noncompliance over time. The sample which was used for this study was taken from a two-wave (one year apart) longitudinal study of preschool-aged children and their mothers (N = 53 dyads). At both times, mothers and their children were invited to a laboratory and engaged in 25 minutes of play involving three episodes of mother-child interaction. Maternal parenting behavior and preschoolers' compliance/noncompliance during play were coded using the Dyadic Parent-Child Interaction Coding System-III (DPICS-III). Maternal codes included positive, neutral, and negative parenting behaviors and child codes were comprised of compliance and noncompliance. The results revealed that during the play session, maternal neutral and negative parenting behavior and preschoolers' compliance were stable over time. In addition, T1 maternal negative parenting behavior was significantly related to T2 child compliance/noncompliance. However, T1 child compliance/noncompliance were not significantly associated with T2 maternal parenting behavior. These findings suggest that during the preschool period, there are unidirectional effects from mothers to children.

The electro-spinning of silk has attracted the attention of researchers in biomedical fields because of 1) the unique properties of silk, including its biocompatibility, and 2) the simplicity of electro-spinning for nanoweb fabrication. Although there are hundreds of varieties of Bombyx mori, the effect of silkworm variety has not been investigated in the study of silk electro-spinning. As an effort to develop electro-spun silk with diverse performances, the effect of Korean silkworm variety on the electro-spinning of silk fibroin (SF) was examined in this study. The regenerated SF solutions showed different viscosities depending on the silkworm variety, which resulted in differences in the maximum electro-spinning rates and diameters of electro-spun SF fibers. However, the crystallization of SF during electro-spinning and the water content of the electro-spun SF webs remained the same between different silkworm varieties.

We examined the antiosteoarthritic effect of the n-hexane extract of Litsea japonica fruit flesh (LJF-HE) in a rat model of monosodium-iodoacetate- (MIA-) induced osteoarthritis. LJF-HE significantly reduced the difference in weight-bearing capabilities of the hind paws between healthy and MIA-treated rats. Histological examination of the knee joints indicated that LJF-HE suppressed cartilage and bone destruction. Additionally, there were decreases in the expression of matrix metalloproteinase-2 and metalloproteinase-9 and cyclooxygenase-2 in the joints. The serum levels of deoxypyridinoline (DPD) and osteocalcin, which are markers of bone metabolism, also decreased. Furthermore, LJF-HE significantly suppressed infiltration of inflammatory cells into the synovium and inhibited the expression of proinflammatory cytokines such as tumor necrosis factor- (TNF-) α, interleukin- (IL-) 1, and IL-6 in the joints and serum. The serum levels of leukotriene B4 and lipoxygenase were also significantly lowered by LJF-HE. Finally, LJF-HE inhibited the production of nitric oxide, prostaglandin E2, IL-6, and TNF-α in lipopolysaccharide-activated macrophages, which might be associated with inhibited phosphorylation of p38 mitogen-activated protein kinase and c-Jun N-terminal kinase. Our data suggest that LJF-HE has an anti-inflammatory effect and may have potential as an antiosteoarthritic agent.

Nature Microbiology 2, 16191 (2016); published online 17 October 2016; corrected 23 January 2017 In the original version of this Article, three author names were spelt incorrectly. The three names have now been corrected to Hye Jung Park, Jae-Hyun Lee and Jung-Won Park in all versions of the Article.

Recently, in the photovoltaic energy integrated monitoring software system, it has more complex, and accordingly may be possible to occur more errors. In this industrial services, a small error can lead to a huge accident to make the power failures. To completely build this system, it should verify whether it is or not stability of software through measuring the full coverage with generating test cases in detail level based on a message sequence model. In this paper, we apply to verify a system stability of this monitoring system with our previous research such as the automatic test case generation based on UML 2.4.1 message-sequence diagram via cause-effect diagram. With this, we extract automatically test cases on coverage.

For safe software development on the solar integrated monitoring system, it is very important how to identify safe behaviors of the system behaviors. Therefore, it needs to test the system behaviors after the software development. To solve this problem, the existing studies have proposed the use case based test coverage analysis at all software development stages [1]. With this method, we identify the test cases based on priority of the system behaviors. In this paper, we proposes automatic test case extraction method based on state diagram among the use case-based test coverage extraction methods. That is, we can use state diagram for a system behaviors with which generates test cases to validate the system. We show an applicative case on the system behaviors of a solar integrated system with this approach.

Today, it is very important issue to high quality of software issue on huge scale of code and time-to-market. In the industrial fields still developers focuses on Code based development. Therefore we try to consider two points of views 1) improving the general developer the bad development habit, and 2) maintenance without design, documentation and code visualization. To solve these problems, we need to make the code visualization of code. In this paper, we suggest how to visualize the inner structure of code, and also how to proceed improvement of quality with constructing the Tool-Chain for visualizing Java code's inner structure. For our practical case, we applied Object Code with NIPA's SW Visualization, and then reduced code complexity through quantitatively analyzing and visualizing code based on setting the basic module unit, the class of object oriented code.

The main purpose of this study was to examine direct and indirect effects of mothers’ depression on preschoolers’ externalizing problem behaviors. A total of 155 mothers with preschoolers aged 3 and 4 years (83 boys and 72 girls) living in Seoul participated in this study. Mothers completed questionnaires on mothers’ depression, parenting behavior, and preschoolers’ externalizing problem behaviors. Direct and indirect pathways from mothers’ depression to preschoolers’ externalizing problem behaviors were analyzed using Structural Equation Model ing (SEM). First, it was revealed that mothers’ depression did not directly affect preschoolers’ externalizing problem behaviors. Second

This study examined the relationships between parenting stress, parenting sense of competence, and parenting behavior in Korean and Japanese mothers of early school-aged children. A sample of 166 Korean mothers and 112 Japanese mothers completed questionnaires. First, in terms of results, Korean mothers reported higher scores in parenting stress, parenting sense of competence, and warmth and neglect than Japanese mothers did. In contrast, Japanese mothers scored higher on intrusiveness than