25 results on '"Bleriot, Camille"'
Search Results
2. Notch signaling regulates macrophage-mediated inflammation in metabolic dysfunction-associated steatotic liver disease
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Guo, Wei, Li, Ziyi, Anagnostopoulos, Gerasimos, Kong, Wan Ting, Zhang, Shuangyan, Chakarov, Svetoslav, Shin, Amanda, Qian, Jiawen, Zhu, Yiwen, Bai, Wenjuan, Cexus, Olivier, Nie, Bin'en, Wang, Jing, Hu, Xiaoyu, Blériot, Camille, Liu, Zhaoyuan, Shen, Baiyong, Venteclef, Nicolas, Su, Bing, and Ginhoux, Florent
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- 2024
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3. Impaired unsaturated fatty acid elongation alters mitochondrial function and accelerates metabolic dysfunction-associated steatohepatitis progression
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Vouilloz, Adrien, Bourgeois, Thibaut, Diedisheim, Marc, Pilot, Thomas, Jalil, Antoine, Le Guern, Naig, Bergas, Victoria, Rohmer, Noéline, Castelli, Florence, Leleu, Damien, Varin, Alexis, de Barros, Jean-Paul Pais, Degrace, Pascal, Rialland, Mickael, Blériot, Camille, Venteclef, Nicolas, Thomas, Charles, and Masson, David
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- 2025
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4. A temporal perspective for tumor-associated macrophage identities and functions
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Blériot, Camille, Dunsmore, Garett, Alonso-Curbelo, Direna, and Ginhoux, Florent
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- 2024
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5. Inflammatory and immune etiology of type 2 diabetes
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Blériot, Camille, Dalmas, Élise, Ginhoux, Florent, and Venteclef, Nicolas
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- 2023
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6. Dynamics and genomic landscape of CD8+ T cells undergoing hepatic priming
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Bénéchet, Alexandre P., De Simone, Giorgia, Di Lucia, Pietro, Cilenti, Francesco, Barbiera, Giulia, Le Bert, Nina, Fumagalli, Valeria, Lusito, Eleonora, Moalli, Federica, Bianchessi, Valentina, Andreata, Francesco, Zordan, Paola, Bono, Elisa, Giustini, Leonardo, Bonilla, Weldy V., Bleriot, Camille, Kunasegaran, Kamini, Gonzalez-Aseguinolaza, Gloria, Pinschewer, Daniel D., Kennedy, Patrick T. F., Naldini, Luigi, Kuka, Mirela, Ginhoux, Florent, Cantore, Alessio, Bertoletti, Antonio, Ostuni, Renato, Guidotti, Luca G., and Iannacone, Matteo
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- 2019
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7. Dying for a Cause: Regulated Necrosis of Tissue-Resident Macrophages upon Infection
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Ginhoux, Florent, Bleriot, Camille, and Lecuit, Marc
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- 2017
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8. Lactation-associated macrophages exist in murine mammary tissue and human milk
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Cansever, Dilay, Petrova, Ekaterina, Krishnarajah, Sinduya, Mussak, Caroline, Welsh, Christina A., Mildenberger, Wiebke, Mulder, Kevin, Kreiner, Victor, Roussel, Elsa, Stifter, Sebastian A., Andreadou, Myrto, Zwicky, Pascale, Puertas Jurado, Nicole, Rehrauer, Hubert, Tan, Ge, Liu, Zhaoyuan, Bleriot, Camille, Ronchi, Francesca, Macpherson, Andrew J., and Ginhoux, Florent
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Macrophages are involved in immune defense, organogenesis and tissue homeostasis. Macrophages contribute to the different phases of mammary gland remodeling during development, pregnancy and involution postlactation. Less is known about the dynamics of mammary gland macrophages in the lactation stage. Here, we describe a macrophage population present during lactation in mice. By multiparameter flow cytometry and single-cell RNA sequencing, we identified a lactation-induced CD11c(+)CX3CR1(+)Dectin-1(+) macrophage population (liMac) that was distinct from the two resident F4/80(hi) and F4/80(lo) macrophage subsets present pregestationally. LiMacs were predominantly monocyte-derived and expanded by proliferation in situ concomitant with nursing. LiMacs developed independently of IL-34, but required CSF-1 signaling and were partly microbiota-dependent. Locally, they resided adjacent to the basal cells of the alveoli and extravasated into the milk. We found several macrophage subsets in human milk that resembled liMacs. Collectively, these findings reveal the emergence of unique macrophages in the mammary gland and milk during lactation., Nature Immunology, 24 (7), ISSN:1529-2908, ISSN:1529-2916
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- 2023
9. Data from Escherichia coli–Specific CXCL13-Producing TFH Are Associated with Clinical Efficacy of Neoadjuvant PD-1 Blockade against Muscle-Invasive Bladder Cancer
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Goubet, Anne-Gaëlle, primary, Lordello, Leonardo, primary, Alves Costa Silva, Carolina, primary, Peguillet, Isabelle, primary, Gazzano, Marianne, primary, Mbogning-Fonkou, Maxime Descartes, primary, Thelemaque, Cassandra, primary, Lebacle, Cédric, primary, Thibault, Constance, primary, Audenet, François, primary, Pignot, Géraldine, primary, Gravis, Gwenaelle, primary, Helissey, Carole, primary, Campedel, Luca, primary, Roupret, Morgan, primary, Xylinas, Evanguelos, primary, Ouzaid, Idir, primary, Dubuisson, Agathe, primary, Mazzenga, Marine, primary, Flament, Caroline, primary, Ly, Pierre, primary, Marty, Virginie, primary, Signolle, Nicolas, primary, Sauvat, Allan, primary, Sbarrato, Thomas, primary, Filahi, Mounia, primary, Davin, Caroline, primary, Haddad, Gabriel, primary, Bou Khalil, Jacques, primary, Bleriot, Camille, primary, Danlos, François-Xavier, primary, Dunsmore, Garett, primary, Mulder, Kevin, primary, Silvin, Aymeric, primary, Raoult, Thibault, primary, Archambaud, Baptiste, primary, Belhechmi, Shaima, primary, Gomperts Boneca, Ivo, primary, Cayet, Nadège, primary, Moya-Nilges, Maryse, primary, Mallet, Adeline, primary, Daillere, Romain, primary, Rouleau, Etienne, primary, Radulescu, Camelia, primary, Allory, Yves, primary, Fieschi, Jacques, primary, Rouanne, Mathieu, primary, Ginhoux, Florent, primary, Le Teuff, Gwénaël, primary, Derosa, Lisa, primary, Marabelle, Aurélien, primary, Van Dorp, Jeroen, primary, Van Dijk, Nick, primary, Van Der Heijden, Michiel S., primary, Besse, Benjamin, primary, Andre, Fabrice, primary, Merad, Miriam, primary, Kroemer, Guido, primary, Scoazec, Jean-Yves, primary, Zitvogel, Laurence, primary, and Loriot, Yohann, primary
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- 2023
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10. Supplementary Figure from Escherichia coli–Specific CXCL13-Producing TFH Are Associated with Clinical Efficacy of Neoadjuvant PD-1 Blockade against Muscle-Invasive Bladder Cancer
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Goubet, Anne-Gaëlle, primary, Lordello, Leonardo, primary, Alves Costa Silva, Carolina, primary, Peguillet, Isabelle, primary, Gazzano, Marianne, primary, Mbogning-Fonkou, Maxime Descartes, primary, Thelemaque, Cassandra, primary, Lebacle, Cédric, primary, Thibault, Constance, primary, Audenet, François, primary, Pignot, Géraldine, primary, Gravis, Gwenaelle, primary, Helissey, Carole, primary, Campedel, Luca, primary, Roupret, Morgan, primary, Xylinas, Evanguelos, primary, Ouzaid, Idir, primary, Dubuisson, Agathe, primary, Mazzenga, Marine, primary, Flament, Caroline, primary, Ly, Pierre, primary, Marty, Virginie, primary, Signolle, Nicolas, primary, Sauvat, Allan, primary, Sbarrato, Thomas, primary, Filahi, Mounia, primary, Davin, Caroline, primary, Haddad, Gabriel, primary, Bou Khalil, Jacques, primary, Bleriot, Camille, primary, Danlos, François-Xavier, primary, Dunsmore, Garett, primary, Mulder, Kevin, primary, Silvin, Aymeric, primary, Raoult, Thibault, primary, Archambaud, Baptiste, primary, Belhechmi, Shaima, primary, Gomperts Boneca, Ivo, primary, Cayet, Nadège, primary, Moya-Nilges, Maryse, primary, Mallet, Adeline, primary, Daillere, Romain, primary, Rouleau, Etienne, primary, Radulescu, Camelia, primary, Allory, Yves, primary, Fieschi, Jacques, primary, Rouanne, Mathieu, primary, Ginhoux, Florent, primary, Le Teuff, Gwénaël, primary, Derosa, Lisa, primary, Marabelle, Aurélien, primary, Van Dorp, Jeroen, primary, Van Dijk, Nick, primary, Van Der Heijden, Michiel S., primary, Besse, Benjamin, primary, Andre, Fabrice, primary, Merad, Miriam, primary, Kroemer, Guido, primary, Scoazec, Jean-Yves, primary, Zitvogel, Laurence, primary, and Loriot, Yohann, primary
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- 2023
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11. Landscape of mast cell populations across organs in mice and humans
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Tauber, Marie, Basso, Lilian, Martin, Jeremy, Bostan, Luciana, Pinto, Marlene Magalhaes, Thierry, Guilhem R., Houmadi, Raissa, Serhan, Nadine, Loste, Alexia, Bleriot, Camille, Kamphuis, Jasper, Grujic, Mirjana, Kjellén, Lena, Pejler, Gunnar, Paul, Carle, Dong, Xinzhong, Galli, Stephen J., Reber, Laurent L., Ginhoux, Florent, Bajenoff, Marc, Gentek, Rebecca, Gaudenzio, Nicolas, Tauber, Marie, Basso, Lilian, Martin, Jeremy, Bostan, Luciana, Pinto, Marlene Magalhaes, Thierry, Guilhem R., Houmadi, Raissa, Serhan, Nadine, Loste, Alexia, Bleriot, Camille, Kamphuis, Jasper, Grujic, Mirjana, Kjellén, Lena, Pejler, Gunnar, Paul, Carle, Dong, Xinzhong, Galli, Stephen J., Reber, Laurent L., Ginhoux, Florent, Bajenoff, Marc, Gentek, Rebecca, and Gaudenzio, Nicolas
- Abstract
Mast cells (MCs) are tissue-resident immune cells that exhibit homeostatic and neuron-associated functions. Here, we combined whole-tissue imaging and single-cell RNA sequencing datasets to generate a pan-organ analysis of MCs in mice and humans at steady state. In mice, we identify two mutually exclusive MC populations, MrgprB2(+) connective tissue-type MCs and MrgprB2(neg) mucosal-type MCs, with specific transcriptomic core signatures. While MrgprB2(+) MCs develop in utero independently of the bone marrow, MrgprB2(neg) MCs develop after birth and are renewed by bone marrow progenitors. In humans, we unbiasedly identify seven MC subsets (MC1-7) distributed across 12 organs with different transcriptomic core signatures. MC1 are preferentially enriched in the bladder, MC2 in the lungs, and MC4, MC6, and MC7 in the skin. Conversely, MC3 and MC5 are shared by most organs but not skin. This comprehensive analysis offers valuable insights into the natural diversity of MC subtypes in both mice and humans. Combining whole-tissue imaging and single-cell RNA sequencing datasets, Tauber et al. present a pan-organ analysis of mast cells in mice and humans at steady state, revealing an unexpected heterogeneity of mast cell populations across tissues and species.
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- 2023
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12. Single-cell analysis of mast cell populations across organs reveals insights into cell origins, anatomical niches and neuron-associated functions.
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Gaudenzio, Nicolas, primary, Tauber, Marie, additional, Basso, Lilian, additional, Wemelle, Eve, additional, Martin, Jeremy, additional, Bostan, Luciana, additional, Pinto, Marlene Magalhaes, additional, Thierry, Guilhem, additional, Houmadi, Raissa, additional, Serhan, Nadine, additional, Loste, Alexia, additional, Bleriot, Camille, additional, Samrout, Celine El, additional, Lasrado, Reena, additional, Kamphuis, Jasper, additional, Pachnis, Vassilis, additional, Grujic, Mirjana, additional, Kjellén, Lena, additional, Pejler, Gunnar, additional, Paul, Carle, additional, Dong, Xinzhong, additional, Galli, Stephen, additional, Reber, Laurent, additional, Ginhoux, Florent, additional, Bajenoff, Marc, additional, Gentek, Rebecca, additional, and Knauff, Claude, additional
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- 2022
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13. Escherichia coli–Specific CXCL13-Producing TFH Are Associated with Clinical Efficacy of Neoadjuvant PD-1 Blockade against Muscle-Invasive Bladder Cancer
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Goubet, Anne-Gaëlle, primary, Lordello, Leonardo, additional, Alves Costa Silva, Carolina, additional, Peguillet, Isabelle, additional, Gazzano, Marianne, additional, Mbogning-Fonkou, Maxime Descartes, additional, Thelemaque, Cassandra, additional, Lebacle, Cédric, additional, Thibault, Constance, additional, Audenet, François, additional, Pignot, Géraldine, additional, Gravis, Gwenaelle, additional, Helissey, Carole, additional, Campedel, Luca, additional, Roupret, Morgan, additional, Xylinas, Evanguelos, additional, Ouzaid, Idir, additional, Dubuisson, Agathe, additional, Mazzenga, Marine, additional, Flament, Caroline, additional, Ly, Pierre, additional, Marty, Virginie, additional, Signolle, Nicolas, additional, Sauvat, Allan, additional, Sbarrato, Thomas, additional, Filahi, Mounia, additional, Davin, Caroline, additional, Haddad, Gabriel, additional, Bou Khalil, Jacques, additional, Bleriot, Camille, additional, Danlos, François-Xavier, additional, Dunsmore, Garett, additional, Mulder, Kevin, additional, Silvin, Aymeric, additional, Raoult, Thibault, additional, Archambaud, Baptiste, additional, Belhechmi, Shaima, additional, Gomperts Boneca, Ivo, additional, Cayet, Nadège, additional, Moya-Nilges, Maryse, additional, Mallet, Adeline, additional, Daillere, Romain, additional, Rouleau, Etienne, additional, Radulescu, Camelia, additional, Allory, Yves, additional, Fieschi, Jacques, additional, Rouanne, Mathieu, additional, Ginhoux, Florent, additional, Le Teuff, Gwénaël, additional, Derosa, Lisa, additional, Marabelle, Aurélien, additional, Van Dorp, Jeroen, additional, Van Dijk, Nick, additional, Van Der Heijden, Michiel S., additional, Besse, Benjamin, additional, Andre, Fabrice, additional, Merad, Miriam, additional, Kroemer, Guido, additional, Scoazec, Jean-Yves, additional, Zitvogel, Laurence, additional, and Loriot, Yohann, additional
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- 2022
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14. Decision letter: Thymic macrophages consist of two populations with distinct localization and origin
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Bleriot, Camille, additional
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- 2021
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15. Identification of a Kupffer cell subset capable of reverting the T cell dysfunction induced by hepatocellular priming
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De Simone, Giorgia, primary, Andreata, Francesco, additional, Bleriot, Camille, additional, Fumagalli, Valeria, additional, Laura, Chiara, additional, Garcia-Manteiga, José M., additional, Di Lucia, Pietro, additional, Gilotto, Stefano, additional, Ficht, Xenia, additional, De Ponti, Federico F., additional, Bono, Elisa B., additional, Giustini, Leonardo, additional, Ambrosi, Gioia, additional, Mainetti, Marta, additional, Zordan, Paola, additional, Bénéchet, Alexandre P., additional, Ravà, Micol, additional, Chakarov, Svetoslav, additional, Moalli, Federica, additional, Bajenoff, Marc, additional, Guidotti, Luca G., additional, Ginhoux, Florent, additional, and Iannacone, Matteo, additional
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- 2021
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16. Tissue-resident ductal macrophages survey the mammary epithelium and facilitate tissue remodelling
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Dawson, Caleb A., primary, Pal, Bhupinder, additional, Vaillant, François, additional, Gandolfo, Luke C., additional, Liu, Zhaoyuan, additional, Bleriot, Camille, additional, Ginhoux, Florent, additional, Smyth, Gordon K., additional, Lindeman, Geoffrey J., additional, Mueller, Scott N., additional, Rios, Anne C., additional, and Visvader, Jane E., additional
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- 2020
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17. Adipose Tissue Vasculature Facilitates Immune Activation of Macrophages Via the CD200-CD200R Axis
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Sulen, André, primary, Li, Xidan, additional, Barreby, Emelie, additional, Azzimato, Valerio, additional, Xu, Connie, additional, Morgantini, Cecilia, additional, Levi, Laura, additional, Jager, Jennifer, additional, Verdeguer, Francisco, additional, Mahú, Inês, additional, Ozcan, Sinem, additional, Domingos, Ana, additional, Li, Qian, additional, Spalding, Kirsty L., additional, Gilleron, Jerome, additional, Cormont, Mireille, additional, Caravaca, April S., additional, Olofsson, Peder S., additional, Näslund, Erik, additional, Laurencikiene, Jurga, additional, Bleriot, Camille, additional, Rydén, Mikael, additional, Ginhoux, Florent, additional, and Aouadi, Myriam, additional
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- 2020
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18. Fate Mapping via Ms4a3-Expression History Traces Monocyte-Derived Cells
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Liu, Zhaoyuan, primary, Gu, Yaqi, additional, Chakarov, Svetoslav, additional, Bleriot, Camille, additional, Kwok, Immanuel, additional, Chen, Xin, additional, Shin, Amanda, additional, Huang, Weijie, additional, Dress, Regine J., additional, Dutertre, Charles-Antoine, additional, Schlitzer, Andreas, additional, Chen, Jinmiao, additional, Ng, Lai Guan, additional, Wang, Honglin, additional, Liu, Zhiduo, additional, Su, Bing, additional, and Ginhoux, Florent, additional
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- 2019
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19. Lipid-Associated Macrophages Control Metabolic Homeostasis in a Trem2-Dependent Manner
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Jaitin, Diego Adhemar, primary, Adlung, Lorenz, additional, Thaiss, Christoph A., additional, Weiner, Assaf, additional, Li, Baoguo, additional, Descamps, Hélène, additional, Lundgren, Patrick, additional, Bleriot, Camille, additional, Liu, Zhaoyuan, additional, Deczkowska, Aleksandra, additional, Keren-Shaul, Hadas, additional, David, Eyal, additional, Zmora, Niv, additional, Eldar, Shai Meron, additional, Lubezky, Nir, additional, Shibolet, Oren, additional, Hill, David A., additional, Lazar, Mitchell A., additional, Colonna, Marco, additional, Ginhoux, Florent, additional, Shapiro, Hagit, additional, Elinav, Eran, additional, and Amit, Ido, additional
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- 2019
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20. Two distinct interstitial macrophage populations coexist across tissues in specific subtissular niches
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Chakarov, Svetoslav, primary, Lim, Hwee Ying, additional, Tan, Leonard, additional, Lim, Sheau Yng, additional, See, Peter, additional, Lum, Josephine, additional, Zhang, Xiao-Meng, additional, Foo, Shihui, additional, Nakamizo, Satoshi, additional, Duan, Kaibo, additional, Kong, Wan Ting, additional, Gentek, Rebecca, additional, Balachander, Akhila, additional, Carbajo, Daniel, additional, Bleriot, Camille, additional, Malleret, Benoit, additional, Tam, John Kit Chung, additional, Baig, Sonia, additional, Shabeer, Muhammad, additional, Toh, Sue-Anne Ee Shiow, additional, Schlitzer, Andreas, additional, Larbi, Anis, additional, Marichal, Thomas, additional, Malissen, Bernard, additional, Chen, Jinmiao, additional, Poidinger, Michael, additional, Kabashima, Kenji, additional, Bajenoff, Marc, additional, Ng, Lai Guan, additional, Angeli, Veronique, additional, and Ginhoux, Florent, additional
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- 2019
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21. Deterministic reprogramming of neutrophils within tumors.
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Ng, Melissa S. F., Kwok, Immanuel, Tan, Leonard, Changming Shi, Cerezo-Wallis, Daniela, Yingrou Tan, Leong, Keith, Calvo, Gabriel F., Yang, Katharine, Yuning Zhang, Jingsi Jin, Ka Hang Liong, Dandan Wu, Rui He, Dehua Liu, Ye Chean Teh, Bleriot, Camille, Caronni, Nicoletta, Zhaoyuan Liu, and Kaibo Duan
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- 2024
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22. Role of the protein RcnB involved in nickel, cobalt and copper homeostasis in the bacterium Escherichia coli
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Bleriot, Camille, Microbiologie, adaptation et pathogénie ( MAP ), Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon ( INSA Lyon ), Université de Lyon-Institut National des Sciences Appliquées ( INSA ) -Institut National des Sciences Appliquées ( INSA ) -Centre National de la Recherche Scientifique ( CNRS ), INSA de Lyon, Agnès Rodrigue, Microbiologie, adaptation et pathogénie (MAP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), and Bleriot, Camille
- Subjects
nickel ,cuivre ,[ SDV.BBM.BC ] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM] ,Métaux ,[SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM] ,cobalt ,bactéries ,[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM] ,homéostasie - Abstract
Metals are ubiquitous on Earth and have become necessary for living organisms like bacteria. However, there is a subtle balance between the life required amount and toxicity resulting from an overload of metal ions. The intracellular level of these ions must be tightly regulated. A strategy often used by cells is to regulate uptake and efflux of metal ions by expressing speci c proteins involved in this traffic. In the bacterium Escherichia coli, nickel and cobalt toxicity is counterbalanced by action of the RcnA efflux pump that permits excess Ni2+ and Co2+ ions to be excluded out of cells. Its expression is under the control of the nickel and cobalt inducible RcnR metalloregulator that represses rcnA transcription in absence of metals. Recently, we identi ed a new gene that we called rcnB (formerly yohN ) involved in this system. This gene is a part of the rcnAB operon and as rcnA, is involved in nickel and cobalt homeostasis. But, unlike rcnA, a mutant strain lacking rcnB is more resistant to nickel and cobalt. This phenotype is associated to a lower accumulation of these ions in the cells. rcnB gene encodes a small monomeric protein of 10 kDa which is found in the periplasmic fraction. Until now, databases do not contain any described homologs. RcnB displayed any Ni2+ or Co2+ binding capacity. On the other hand, RcnB can bind both Cu+ and Cu2+ ions in vitro. It is equally true in vivo and a link with copper homeostasis was found based on the copper resistance systems of E. coli. This is of particular interest because copper confers particular redox properties to proteins which could be directly related to the biological function of the RcnB protein. Further studies are required to precise the role of RcnB but this work provides the identifi cation and characterization of the first member of a new family of accessory proteins tied to metal ions efflux pumps., Les métaux sont omniprésents sur la planète et sont devenus nécessaires à tous les organismes vivants au cours de l'évolution. Cependant, la quantité de métal présent dans les cellules doit être très finement régulée car une quantité trop importante de métaux peut générer une toxicité. Pour assurer cette homéostasie, les cellules doivent être capables de réguler l'import et l'efflux des métaux par l'expression dynamique de protéines spécifi ques impliquées dans ces flux ioniques. Chez la bactérie modèle Escherichia coli, la toxicité du nickel et du cobalt est neutralisée par l'action de la pompe RcnA, protéine membranaire capable d'exporter les ions Ni2+ et Co2+ hors de la cellule. La production de RcnA est contrôlée par le métallorégulateur RcnR capable de détecter ces ions en cas d'excès et d'induire, en réponse à ce signal, l'expression du gène rcnA. Récemment, notre équipe a identi fié un nouveau gène que nous avons rebaptisé rcnB (précédemment yohN ). Ce gène fait partie de l'opéron rcnAB et est, comme rcnA, impliqué dans l'homéostasie des ions Ni2+ et Co2+. A l'inverse de rcnA, une souche mutante délétée de rcnB est plus résistante au nickel et au cobalt et cela est directement corrélée à une plus faible accumulation de métal dans les cellules. Le gène rcnB code pour une petite protéine monomérique de 10 kDa localisée dans le périplasme et ne possédant pas d'homologues décrits à l'heure actuelle. Aucune fixation des ions Ni2+ et Co2+ n'a été mise en évidence pour la protéine RcnB. Elle peut en revanche interagir avec les ions Cu+ et Cu2+ aussi bien in vitro que in vivo et un lien avec les systèmes de résistance au cuivre de E. coli a été mis en évidence. Or, le cuivre confère des propriétés d'oxydo-réduction particulières aux protéines qui pourraient être directement liées à la fonction de RcnB. Des investigations complémentaires sont nécessaires pour préciser la fonction exacte de RcnB, mais ce travail a mis en évidence le premier membre d'une nouvelle famille de protéines accessoires associées aux pompes d'efflux des ions métalliques.
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- 2010
23. Dynamics and genomic landscape of CD8+T cells undergoing hepatic priming
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Bénéchet, Alexandre P., De Simone, Giorgia, Di Lucia, Pietro, Cilenti, Francesco, Barbiera, Giulia, Le Bert, Nina, Fumagalli, Valeria, Lusito, Eleonora, Moalli, Federica, Bianchessi, Valentina, Andreata, Francesco, Zordan, Paola, Bono, Elisa, Giustini, Leonardo, Bonilla, Weldy V., Bleriot, Camille, Kunasegaran, Kamini, Gonzalez-Aseguinolaza, Gloria, Pinschewer, Daniel D., Kennedy, Patrick T. F., Naldini, Luigi, Kuka, Mirela, Ginhoux, Florent, Cantore, Alessio, Bertoletti, Antonio, Ostuni, Renato, Guidotti, Luca G., and Iannacone, Matteo
- Abstract
The responses of CD8+T cells to hepatotropic viruses such as hepatitis B range from dysfunction to differentiation into effector cells, but the mechanisms that underlie these distinct outcomes remain poorly understood. Here we show that priming by Kupffer cells, which are not natural targets of hepatitis B, leads to differentiation of CD8+T cells into effector cells that form dense, extravascular clusters of immotile cells scattered throughout the liver. By contrast, priming by hepatocytes, which are natural targets of hepatitis B, leads to local activation and proliferation of CD8+T cells but not to differentiation into effector cells; these cells form loose, intravascular clusters of motile cells that coalesce around portal tracts. Transcriptomic and chromatin accessibility analyses reveal unique features of these dysfunctional CD8+T cells, with limited overlap with those of exhausted or tolerant T cells; accordingly, CD8+T cells primed by hepatocytes cannot be rescued by treatment with anti-PD-L1, but instead respond to IL-2. These findings suggest immunotherapeutic strategies against chronic hepatitis B infection.
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- 2019
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24. Dynamics and genomic landscape of CD8+ T cells undergoing hepatic priming
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Patrick T F Kennedy, Valentina Bianchessi, Antonio Bertoletti, Mirela Kuka, Luca G. Guidotti, Giorgia De Simone, Giulia Barbiera, Francesco Andreata, Florent Ginhoux, Elisa Bono, Alessio Cantore, Leonardo Giustini, Eleonora Lusito, Weldy V. Bonilla, Alexandre P. Benechet, Matteo Iannacone, Paola Zordan, Kamini Kunasegaran, Daniel D. Pinschewer, Pietro Di Lucia, Camille Blériot, Renato Ostuni, Luigi Naldini, Francesco Cilenti, Nina Le Bert, Gloria González-Aseguinolaza, Valeria Fumagalli, Federica Moalli, Bénéchet, Alexandre P, De Simone, Giorgia, Di Lucia, Pietro, Cilenti, Francesco, Barbiera, Giulia, Le Bert, Nina, Fumagalli, Valeria, Lusito, Eleonora, Moalli, Federica, Bianchessi, Valentina, Andreata, Francesco, Zordan, Paola, Bono, Elisa, Giustini, Leonardo, Bonilla, Weldy V, Bleriot, Camille, Kunasegaran, Kamini, Gonzalez-Aseguinolaza, Gloria, Pinschewer, Daniel D, Kennedy, Patrick T F, Naldini, Luigi, Kuka, Mirela, Ginhoux, Florent, Cantore, Alessio, Bertoletti, Antonio, Ostuni, Renato, Guidotti, Luca G, Iannacone, Matteo, Benechet, A, De Simone, G, Di Lucia, P, Cilenti, F, Barbiera, G, Le Bert, N, Fumagalli, V, Lusito, E, Moalli, F, Bianchessi, V, Andreata, F, Zordan, P, Bono, E, Giustini, L, Bonilla, W, Bleriot, C, Kunasegaran, K, Gonzalez-Aseguinolaza, G, Pinschewer, D, Kennedy, P, Naldini, L, Kuka, M, Ginhoux, F, Cantore, A, Bertoletti, A, Ostuni, R, Guidotti, L, and Iannacone, M
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Male ,0301 basic medicine ,Hepatitis B virus ,Kupffer Cells ,Cellular differentiation ,Priming (immunology) ,CD8-Positive T-Lymphocytes ,Biology ,Lymphocyte Activation ,Article ,B7-H1 Antigen ,Immune tolerance ,Transcriptome ,Intravital microscopy ,Mice ,03 medical and health sciences ,Cross-Priming ,0302 clinical medicine ,HBV ,Immune Tolerance ,Animals ,Humans ,Cytotoxic T cell ,Multidisciplinary ,Effector ,Cell Differentiation ,Hepatitis B ,Chromatin ,3. Good health ,Cell biology ,030104 developmental biology ,030220 oncology & carcinogenesis ,Hepatocytes ,Interleukin-2 ,Female ,CD8+ T cell ,CD8 - Abstract
The responses of CD8(+) T cells to hepatotropic viruses such as hepatitis B range from dysfunction to differentiation into effector cells, but the mechanisms that underlie these distinct outcomes remain poorly understood. Here we show that priming by Kupffer cells, which are not natural targets of hepatitis B, leads to differentiation of CD8(+) T cells into effector cells that form dense, extravascular clusters of immotile cells scattered throughout the liver. By contrast, priming by hepatocytes, which are natural targets of hepatitis B, leads to local activation and proliferation of CD8(+) T cells but not to differentiation into effector cells; these cells form loose, intravascular clusters of motile cells that coalesce around portal tracts. Transcriptomic and chromatin accessibility analyses reveal unique features of these dysfunctional CD8(+) T cells, with limited overlap with those of exhausted or tolerant T cells; accordingly, CD8(+) T cells primed by hepatocytes cannot be rescued by treatment with anti-PD-L1, but instead respond to IL-2. These findings suggest immunotherapeutic strategies against chronic hepatitis B infection.
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25. Trem2-expressing multinucleated giant macrophages are a biomarker of good prognosis in head and neck squamous cell carcinoma.
- Author
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Gessain G, Anzali AA, Lerousseau M, Mulder K, Bied M, Auperin A, Stockholm D, Signolle N, Sassi F, Marques Da Costa ME, Marchais A, Sayadi A, Weidner D, Uderhardt S, Blampey Q, Nakkireddy SR, Broutin S, Dutertre CA, Busson P, Walter T, Marhic A, Moya-Plana A, Guerlain J, Breuskin I, Casiraghi O, Gorphe P, Classe M, Scoazec JY, Bleriot C, and Ginhoux F
- Abstract
Patients with head and neck squamous cell carcinomas (HNSCC) often have poor outcomes due to suboptimal risk-management and treatment strategies; yet integrating novel prognostic biomarkers into clinical practice is challenging. Here, we report the presence of multinucleated giant cells (MGC) - a type of macrophages - in tumors from patients with HNSCC, which are associated with a favorable prognosis in treatment-naive and preoperative-chemotherapy-treated patients. Importantly, MGC density increased in tumors following preoperative therapy, suggesting a role of these cells in the anti-tumoral response. To enable clinical translation of MGC density as a prognostic marker, we developed a deep-learning model to automate its quantification on routinely stained pathological whole slide images. Finally, we used spatial transcriptomic and proteomic approaches to describe the MGC-related tumor microenvironment and observed an increase in central memory CD4 T cells. We defined an MGC-specific signature resembling to TREM2-expressing mononuclear tumor associated macrophages, which co-localized in keratin tumor niches.
- Published
- 2024
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