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1. ATR‐FTIR spectroscopy imaging of bone repair in mandibular laser‐osteotomy.

Author

Benetti, Carolina, Blay, Alberto, Correa, Luciana, Verlangieri, Marco Aurelio, dos Santos, Moisés O., Kazarian, Sergei G., and Zezell, Denise M.

Abstract

The aim of this study was to verify the effectiveness of attenuated total reflectance‐fourier transform infrared (ATR‐FTIR) spectroscopy in the characterization of bone repair in mandibular osteotomy using erbium, chromium‐doped yttrium, scandium, gallium and garnet (Er,Cr:YSGG) laser and multilaminate drill on each side. Two mandible bone fragments were removed from 30 rabbits, and the process of bone repair was studied immediately, 3, 7, 15, 21, and 28 days after the surgery. The histological analysis allowed detecting differences in the early stages of tissue repair after bone cutting performed with the Er,Cr:YSGG laser or multilaminate drill. The ATR‐FTIR spectroscopy technique was sensitive to changes in the organic content of bone tissue repair process. [ABSTRACT FROM AUTHOR]

Published
2024
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3. Performance of Polydioxanone-Based Membrane in Association with 3D-Printed Bioceramic Scaffolds in Bone Regeneration

Author

Pitol-Palin, Letícia, primary, Frigério, Paula Buzo, additional, Moura, Juliana, additional, Pilatti, Livia, additional, de Oliveira, Letícia Marques Jordão, additional, Matsubara, Elaine Yoshiko, additional, Tunchel, Samy, additional, Shibli, Jamil Awad, additional, Blay, Alberto, additional, Saska, Sybele, additional, and Okamoto, Roberta, additional

Published
2022
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7. Performance of Polydioxanone-Based Membrane in Association with 3D-Printed Bioceramic Scaffolds in Bone Regeneration.

Author

Pitol-Palin, Letícia, Frigério, Paula Buzo, Moura, Juliana, Pilatti, Livia, de Oliveira, Letícia Marques Jordão, Matsubara, Elaine Yoshiko, Tunchel, Samy, Shibli, Jamil Awad, Blay, Alberto, Saska, Sybele, and Okamoto, Roberta

Subjects
BONE regeneration, GUIDED bone regeneration, X-ray computed microtomography, BONE growth, POLYCAPROLACTONE
Abstract

This study evaluated the bioactivity of 3D-printed β-tricalcium phosphate (β-TCP) scaffolds or hydroxyapatite (HA) scaffolds associated with polydioxanone (PDO) membrane (Plenum® Guide) for guided bone regeneration in rats. Fifty-four rats were divided into three groups (n = 18 animals): autogenous bone + PDO membrane (Auto/PG); 3D-printed β-TCP + PDO membrane (TCP/PG); and 3D-printed HA + PDO membrane (HA/PG). A surgical defect in the parietal bone was made and filled with the respective scaffolds and PDO membrane. The animals were euthanized 7, 30, and 60 days after the surgical procedure for micro-CT, histomorphometric, and immunolabeling analyses. Micro-CT showed an increase in trabecular thickness and a decrease in trabecular separation, even with similar bone volume percentages between TCP/PG and HA/PG vs. Auto/PG. Histometric analysis showed increased bone formation at 30 days in the groups compared to 7 days postoperatively. Immunolabeling analysis showed an increase in proteins related to bone formation at 30 days, and both groups showed a similar immunolabeling pattern. This study concludes that 3D-printed scaffolds associated with PDO membrane (Plenum® Guide) present similar results to autogenous bone for bone regeneration. [ABSTRACT FROM AUTHOR]

Published
2023
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8. Polydioxanone-Based Membranes for Bone Regeneration

Author

Saska, Sybele, primary, Pilatti, Livia, additional, Silva, Edvaldo Santos de Sousa, additional, Nagasawa, Magda Aline, additional, Câmara, Diana, additional, Lizier, Nelson, additional, Finger, Eduardo, additional, Dyszkiewicz Konwińska, Marta, additional, Kempisty, Bartosz, additional, Tunchel, Samy, additional, Blay, Alberto, additional, and Shibli, Jamil Awad, additional

Published
2021
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12. Volumetric Changes in Anterior Maxillary Reconstruction using DBBM Associated with PRF Membrane and Titanium Mesh - case series.

Author

Tunchel, Samy, Parize, Graziele, Blay, Alberto, Duailibi Neto, Eduardo Felippe, Yeon Jung Kim, and Pallos, Debora

Abstract

Aim: This study aimed to assess the horizontal and volumetric bone augmentation obtained by using deproteinized bovine bone mineral (DBBM) associated with platelet-rich fibrin (PRF) membrane and titanium mesh at anterior atrophic region of the maxilla. Materials and methods: Eight healthy patients requiring horizontal and vertical bone reconstructions were included. The amount of horizontal bone gain was measured on computed tomographic images and the thickness at three levels (7,11,13 mm from the crest) before and six months after reconstructive surgery. Each graft was plotted on sagittal image at the mid-face by using the OnDemand 3D Dental TM software and volumetric analysis was performed by using the InVesalius 3.0 software. Results: The initial height was 15.61 ± 4.3 mm and the final one was 17.94 ± 5.2 mm (p = 0.001), resulting in a gain of 2.33 mm and the initial thickness 5.962; 6.762; 7.918 (7,11,13 mm from the crest) change to 8.627; 10.05; 10.63 respectively. The volume increased 42.939± 16.856 mm3, which corresponds to a variation of 45 - 160%. depending on the defect. A total of 20 implants were successfully installed. Conclusion: The use of DBBM, PRF and titanium mesh is a safe choice with promising results. [ABSTRACT FROM AUTHOR]

Published
2022

21. Viability of autogenous bone grafts obtained by using bone collectors: histological and microbiological study

Author

Blay, Alberto, Tunchel, Samy, and Sendyk, Wilson Roberto

Subjects
Dental implantation, Bone transplantation, Regeneração óssea, Transplante ósseo, Bone regeneration, Implante dentário
Abstract

The use of autogenous bone grafts is considered to be the best choice for reconstructive surgery. In the periodontal literature, the utilization of osseous coagulum was suggested by the end of the sixties. The purpose of this study is to consider the use of bone collectors (bone traps) as an alternative method for obtaining material to fill small bone imperfections, such as fenestrations and dehiscences. Thirty samples were obtained from bone drilling during fixture installation in patients (13 men and 17 women, with an average age of 54 years) requiring treatment at the Department of Periodontology and Implant Dentistry, University of Santo Amaro. These samples were fixed in 10% neutral formaldehyde for 24 hours and subjected to histological preparation, in order to evaluate the presence of viable osteoblasts. In addition, the material was placed in a fluid thioglycolate medium and incubated for 24 hours at 36 ± 1°C in aerobiosis and anaerobiosis. Bacterial growth evaluation was made by using six different culture media (MacConkey agar, blood agar base, mannitol salt agar, Anaerokit LTD medium, Anaerokit LTD - bile medium, Anaerinsol). The results show that, if proper care is taken to prevent saliva contamination during the surgical procedure, this method of collecting autogenous bone may be useful in situations where small amounts of bone are required. A utilização de enxertos autógenos é considerada a melhor opção nos tratamentos cirúrgicos de reconstrução óssea. Na literatura periodontal, a utilização de coágulo ósseo foi sugerida no final da década de 60. O objetivo deste estudo é considerar a utilização de coletores para osso como um método alternativo de se obter osso autógeno para preenchimento de defeitos ósseos como fenestrações e deiscências. Trinta amostras foram obtidas no processo de perfuração do tecido ósseo, durante a instalação de implantes em pacientes (13 homens e 17 mulheres, com média etária de 54 anos) que foram submetidos a tratamento na Disciplina de Periodontia e Implantodontia da Universidade de Santo Amaro. Essas amostras foram fixadas em solução de formol neutro a 10% por 24 horas para serem analisadas histologicamente com o intuito de avaliar a presença de osteoblastos viáveis. Além das amostras fixadas, também foram obtidos espécimens que foram incubados em aerobiose e em anaerobiose, em meio de tioglicolato por 24 h a 36 ± 1°C. A avaliação do crescimento bacteriano foi feita através de seis meios seletivos de cultura (ágar MacConkey, ágar-sangue, ágar manitol, meio Anaerokit LTD, meio Anaerokit LTD - bile e Anaerinsol). Os resultados mostraram que, se forem tomados certos cuidados para prevenir a contaminação com saliva durante o procedimento cirúrgico, este método de coletar osso autógeno pode ser útil em situações em que pequenas quantidades de osso são necessárias.

Published
2003

23. Efeitos da radiacao laser em baixa intensidade no mecanismo de osseointegracao de implantes: estudo 'in vivo'

Author

BLAY, ALBERTO, Eduardo de Bortoli Groth, and Denise Maria Zezell

Subjects
bone tissues, rabbits, dentistry, irradiation, bioassay, laser radiation, implants, response modifying factors, lasers, tibia, teeth
Abstract

Made available in DSpace on 2014-10-09T12:45:19Z (GMT). No. of bitstreams: 0 Made available in DSpace on 2014-10-09T14:06:07Z (GMT). No. of bitstreams: 1 07430.pdf: 1129491 bytes, checksum: 0b39089585cf6b2242e38c4bb6ccf19e (MD5) Dissertacao (Mestrado Profissionalizante em Lasers em Odontologia) IPEN/D-MPLO Intituto de Pesquisas Energeticas e Nucleares, IPEN/CNEN-SP; Faculdade de Odontologia, Universidade de Sao Paulo

Published
2001

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